CN107746825A - Beneficial to the culture medium of streptococcus pneumonia fast breeding - Google Patents
Beneficial to the culture medium of streptococcus pneumonia fast breeding Download PDFInfo
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- CN107746825A CN107746825A CN201711236204.5A CN201711236204A CN107746825A CN 107746825 A CN107746825 A CN 107746825A CN 201711236204 A CN201711236204 A CN 201711236204A CN 107746825 A CN107746825 A CN 107746825A
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
The invention discloses the culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight ratio：Carbon source is：Jerusalem artichoke, 10~35 parts, ganoderma lucidum, 10~30 parts, bagasse, 15~40 parts；Nitrogen source is：Cottonseed meal, 20~80 parts, peptone, 15~45 parts, shrimp powder, 20~50 parts, corn steep liquor, 15~35 parts, 10~30 parts of wheat bran；Inorganic salts and trace element：MgSO4, 0.20~1.5g/L；NaCl, 2.5~12.5g/L；K2HPO4, 0.1~2.5g/L；ZnSO4, 0.1~0.2g/L；FeSO4, 0.01~0.12g/L；Nicotinic acid：0.2~1.2g/L；Adenine：3.5~8.5g/L；Cysteine, 0.8~2.0g/L；Water, 1000 parts.Present invention facilitates streptococcus pneumonia multiplication rate to be obviously improved, while is not susceptible to microbiological contamination problem.
Description
Technical field
The present invention relates to microbial technology field, and in particular to beneficial to the culture medium of streptococcus pneumonia fast breeding.
Background technology
Streptococcus pneumonia is in 1881 first by Pasteur (Louis Pasteur) and G.M.Sternberg respectively in method
State and the U.S. isolate from patient's sputum.Positive for Grain stain, thalline is like spearhead shape, pair in pairs or into short catenation
Coccus, toxic strain thalline have the pod membrane that chemical composition is polysaccharide outside.This bacterium is carried in 5%~10% normal person's upper respiratory tract, is had
Strain is the important pathogen for causing human diseases.The bacterial strain has certain application in biological technical field, but conventional
Culture medium in streptococcus pneumonia growth rate it is slower, while easily occur microbiological contamination phenomenon.
The content of the invention
The technical problems to be solved by the invention are currently used in the culture medium of culture streptococcus pneumonia, streptococcus pneumonia
Growth rate is slower, and microbiological contamination phenomenon easily occurs, and it is an object of the present invention to provide culture medium beneficial to streptococcus pneumonia fast breeding, promotes
Streptococcus pneumonia multiplication rate is obviously improved, while is not susceptible to microbiological contamination problem.
The present invention is achieved through the following technical solutions：
Beneficial to the culture medium of streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight ratio：
Carbon source is：Jerusalem artichoke, 10~35 parts, ganoderma lucidum, 10~30 parts, bagasse, 15~40 parts；
Nitrogen source is：Cottonseed meal, 20~80 parts, peptone, 15~45 parts, shrimp powder, 20~50 parts, corn steep liquor, 15~
35 parts, 10~30 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 0.20~1.5g/L；NaCl, 2.5~12.5g/L；K2HPO4, 0.1~
2.5g/L；ZnSO4, 0.1~0.2g/L；FeSO4, 0.01~0.12g/L；Nicotinic acid：0.2~1.2g/L；Adenine：3.5~
8.5g/L；Cysteine, 0.8~2.0g/L；
12~20 parts of agar；
Water, 1000 parts.
Preferably, the culture medium raw material composition is by weight ratio：
Source is：Jerusalem artichoke, 15~30 parts, ganoderma lucidum, 15~25 parts, bagasse, 20~35 parts；
Nitrogen source is：Cottonseed meal, 40~65 parts, peptone, 25~35 parts, shrimp powder, 35~45 parts, corn steep liquor, 20~
30 parts, 15~25 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 0.8~1.2g/L；NaCl, 5.5~10.5g/L；K2HPO4, 0.9~1.5g/
L；ZnSO4, 0.15~0.18g/L；FeSO4, 0.05~0.10g/L；Nicotinic acid：0.6~0.9g/L；Adenine：5.5~6.5g/L；
Cysteine, 1.2~1.8g/L；
13~18 parts of agar；
Water, 1000 parts.
Preferably, the culture medium raw material composition is by weight ratio：
Source is：Jerusalem artichoke, 25 parts, ganoderma lucidum, 20 parts, bagasse, 28 parts；
Nitrogen source is：Cottonseed meal, 60 parts, peptone, 30 parts, shrimp powder, 40 parts, corn steep liquor, 25 parts, 20 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 1.0g/L；NaCl, 6.5g/L；K2HPO4, 1.2g/L；ZnSO4, 0.16g/L；
FeSO4, 0.08g/L；Nicotinic acid：0.8g/L；Adenine：6.0g/L；Cysteine, 1.6g/L；
15 parts of agar；
Water, 1000 parts.
Preferably, the culture medium regulation pH value is 7.2~7.6.
The present invention compared with prior art, it is quick to have the following advantages and advantages that the present invention is beneficial to streptococcus pneumonia
The culture medium of propagation,
The invention provides the culture medium beneficial to streptococcus pneumonia fast breeding, improves to streptococcus pneumonia solid culture
The formula of base, using culture medium dispensing provided by the invention, promote streptococcus pneumonia multiplication rate and be obviously improved, be not easy simultaneously
Generation microbiological contamination problem.
Embodiment
For the object, technical solutions and advantages of the present invention are more clearly understood, with reference to embodiment, the present invention is made
Further to describe in detail, exemplary embodiment of the invention and its explanation are only used for explaining the present invention, are not intended as to this
The restriction of invention.
Embodiment 1
The invention provides a kind of culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight
Amount part, which matches, is：
Carbon source is：Jerusalem artichoke, 10g, ganoderma lucidum, 10g, bagasse, 15g；
Nitrogen source is：Cottonseed meal, 20g, peptone, 15g, shrimp powder, 20g, corn steep liquor, 15g, wheat bran 10g；
Inorganic salts and trace element：MgSO4, 0.20g/L；NaCl, 2.5g/L；K2HPO4, 0.1g/L；ZnSO4, 0.1g/L；
FeSO4, 0.01g/L；Nicotinic acid：0.2g/L；Adenine：3.5g/L；Cysteine, 0.8g/L；
Agar, 12g；
Water, 1000ml.Culture medium regulation pH value is 7.2~7.6.
Embodiment 2
The invention provides a kind of culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight
Amount part, which matches, is：
Carbon source is：Jerusalem artichoke, 35g, ganoderma lucidum, 30g, bagasse, 40g；
Nitrogen source is：Cottonseed meal, 80g, peptone, 45g, shrimp powder, 50g, corn steep liquor, 35g, wheat bran, 30g；
Inorganic salts and trace element：MgSO4, 1.5g/L；NaCl, 12.5g/L；K2HPO4, 2.5g/L；ZnSO4, 0.2g/L；
FeSO4, 0.12g/L；Nicotinic acid：1.2g/L；Adenine：8.5g/L；Cysteine, 2.0g/L；
Agar, 20g
Water, 1000ml.Culture medium regulation pH value is 7.2~7.6.
Embodiment 3
The invention provides a kind of culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight
Amount part, which matches, is：
Source is：Jerusalem artichoke, 15g, ganoderma lucidum, 15g, bagasse, 20g；
Nitrogen source is：Cottonseed meal, 40g, peptone, 25g, shrimp powder, 35g, corn steep liquor, 20g, wheat bran 15g；
Inorganic salts and trace element：MgSO4, 0.8g/L；NaCl, 5.5g/L；K2HPO4, 0.9g/L；ZnSO4, 0.15g/L；
FeSO4, 0.05g/L；Nicotinic acid：0.6g/L；Adenine：5.5g/L；Cysteine, 1.2g/L；
Agar, 13g；
Water, 1000ml.Culture medium regulation pH value is 7.2~7.6.
Embodiment 4
The invention provides a kind of culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight
Amount part, which matches, is：
Source is：Jerusalem artichoke, 30g, ganoderma lucidum, 25g, bagasse, 35g；
Nitrogen source is：Cottonseed meal, 65g, peptone, 35g, shrimp powder, 45g, corn steep liquor, 30g, wheat bran, 25g；
Inorganic salts and trace element：MgSO4, 1.2g/L；NaCl, 10.5g/L；K2HPO4, 1.5g/L；ZnSO4, 0.18g/
L；FeSO4, 0.10g/L；Nicotinic acid：0.9g/L；Adenine：6.5g/L；Cysteine, 1.8g/L；
Agar, 18g；
Water, 1000ml.Culture medium regulation pH value is 7.2~7.6.
Embodiment 5
The invention provides a kind of culture medium beneficial to streptococcus pneumonia fast breeding, the culture medium raw material composition is by weight
Amount part, which matches, is：
Source is：Jerusalem artichoke, 25g, ganoderma lucidum, 20g, bagasse, 28g；
Nitrogen source is：Cottonseed meal, 60g, peptone, 30g, shrimp powder, 40g, corn steep liquor, 25g, wheat bran 20g；
Inorganic salts and trace element：MgSO4, 1.0g/L；NaCl, 6.5g/L；K2HPO4, 1.2g/L；ZnSO4, 0.16g/L；
FeSO4, 0.08g/L；Nicotinic acid：0.8g/L；Adenine：6.0g/L；Cysteine, 1.6g/L；
Agar 15g；
Water, 1000ml.
The culture medium regulation pH value is 7.2~7.6.Culture medium regulation pH value is 7.2~7.6.
Above-described embodiment, the purpose of the present invention, technical scheme and beneficial effect are carried out further
Describe in detail, should be understood that the embodiment that the foregoing is only the present invention, be not intended to limit the present invention
Protection domain, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc., all should include
Within protection scope of the present invention.
Claims (4)
1. beneficial to the culture medium of streptococcus pneumonia fast breeding, it is characterised in that the culture medium raw material composition is matched somebody with somebody by weight
Than for：
Carbon source is：Jerusalem artichoke, 10~35 parts, ganoderma lucidum, 10~30 parts, bagasse, 15~40 parts；
Nitrogen source is：Cottonseed meal, 20~80 parts, peptone, 15~45 parts, shrimp powder, 20~50 parts, corn steep liquor, 15~35 parts,
10~30 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 0.20~1.5g/L；NaCl, 2.5~12.5g/L；K2HPO4, 0.1~2.5g/L；
ZnSO4, 0.1~0.2g/L；FeSO4, 0.01~0.12g/L；Nicotinic acid：0.2~1.2g/L；Adenine：3.5~8.5g/L；Half Guang
Propylhomoserin, 0.8~2.0g/L；
12~20 parts of agar；
Water, 1000 parts.
2. the culture medium according to claim 1 beneficial to streptococcus pneumonia fast breeding, it is characterised in that the culture medium
Raw material forms：
Source is：Jerusalem artichoke, 15~30 parts, ganoderma lucidum, 15~25 parts, bagasse, 20~35 parts；
Nitrogen source is：Cottonseed meal, 40~65 parts, peptone, 25~35 parts, shrimp powder, 35~45 parts, corn steep liquor, 20~30 parts,
15~25 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 0.8~1.2g/L；NaCl, 5.5~10.5g/L；K2HPO4, 0.9~1.5g/L；
ZnSO4, 0.15~0.18g/L；FeSO4, 0.05~0.10g/L；Nicotinic acid：0.6~0.9g/L；Adenine：5.5~6.5g/L；Half
Cystine, 1.2~1.8g/L；
13~18 parts of agar；
Water, 1000 parts.
3. the culture medium according to claim 1 beneficial to streptococcus pneumonia fast breeding, it is characterised in that its feature exists
In the culture medium raw material composition is by weight ratio：
Source is：Jerusalem artichoke, 25 parts, ganoderma lucidum, 20 parts, bagasse, 28 parts；
Nitrogen source is：Cottonseed meal, 60 parts, peptone, 30 parts, shrimp powder, 40 parts, corn steep liquor, 25 parts, 20 parts of wheat bran；
Inorganic salts and trace element：MgSO4, 1.0g/L；NaCl, 6.5g/L；K2HPO4, 1.2g/L；ZnSO4, 0.16g/L；
FeSO4, 0.08g/L；Nicotinic acid：0.8g/L；Adenine：6.0g/L；Cysteine, 1.6g/L；
15 parts of agar；
Water, 1000 parts.
4. the culture medium according to claim 1 beneficial to streptococcus pneumonia fast breeding, it is characterised in that the culture medium
It is 7.2~7.6 to adjust pH value.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711236204.5A CN107746825A (en) | 2017-11-30 | 2017-11-30 | Beneficial to the culture medium of streptococcus pneumonia fast breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711236204.5A CN107746825A (en) | 2017-11-30 | 2017-11-30 | Beneficial to the culture medium of streptococcus pneumonia fast breeding |
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| Publication Number | Publication Date |
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| CN107746825A true CN107746825A (en) | 2018-03-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711236204.5A Pending CN107746825A (en) | 2017-11-30 | 2017-11-30 | Beneficial to the culture medium of streptococcus pneumonia fast breeding |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844353A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Streptococcus thermophilus jerusalem artichoke juice enriched medium |
| CN102093963A (en) * | 2010-11-26 | 2011-06-15 | 兰州生物制品研究所 | Method for culturing streptococcus penumoniae rich in teichoic acid |
| CN102257127A (en) * | 2008-12-18 | 2011-11-23 | 惠氏有限责任公司 | Method for controlling streptococcus pneumoniae polysaccharide molecular weight using carbon |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
| CN106867926A (en) * | 2015-12-14 | 2017-06-20 | 天津植草园生物科技有限公司 | A kind of culture medium for streptococcus pneumonia |
-
2017
- 2017-11-30 CN CN201711236204.5A patent/CN107746825A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1844353A (en) * | 2006-05-12 | 2006-10-11 | 河北农业大学 | Streptococcus thermophilus jerusalem artichoke juice enriched medium |
| CN102257127A (en) * | 2008-12-18 | 2011-11-23 | 惠氏有限责任公司 | Method for controlling streptococcus pneumoniae polysaccharide molecular weight using carbon |
| CN102093963A (en) * | 2010-11-26 | 2011-06-15 | 兰州生物制品研究所 | Method for culturing streptococcus penumoniae rich in teichoic acid |
| CN104293699A (en) * | 2014-09-17 | 2015-01-21 | 山东大学 | Streptococcus thermophilus with synthetic capability of gamma-aminobutyric acid and glutathione and application of streptococcus thermophilus |
| CN106867926A (en) * | 2015-12-14 | 2017-06-20 | 天津植草园生物科技有限公司 | A kind of culture medium for streptococcus pneumonia |
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Application publication date: 20180302 |