CN107518879A - A kind of fluoroscopic imaging device and method - Google Patents
A kind of fluoroscopic imaging device and method Download PDFInfo
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- CN107518879A CN107518879A CN201710941707.6A CN201710941707A CN107518879A CN 107518879 A CN107518879 A CN 107518879A CN 201710941707 A CN201710941707 A CN 201710941707A CN 107518879 A CN107518879 A CN 107518879A
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- 238000003384 imaging method Methods 0.000 title claims abstract description 39
- 238000000034 method Methods 0.000 title claims abstract description 17
- 230000003287 optical effect Effects 0.000 claims abstract description 93
- 238000000799 fluorescence microscopy Methods 0.000 claims abstract description 26
- 230000011664 signaling Effects 0.000 claims abstract description 23
- 230000003595 spectral effect Effects 0.000 claims description 19
- 230000004927 fusion Effects 0.000 claims description 11
- 238000000354 decomposition reaction Methods 0.000 claims description 10
- 239000013307 optical fiber Substances 0.000 claims description 7
- 230000009466 transformation Effects 0.000 claims description 6
- 239000000835 fiber Substances 0.000 claims description 3
- 229910052736 halogen Inorganic materials 0.000 claims description 2
- 125000005843 halogen group Chemical group 0.000 claims description 2
- 238000001228 spectrum Methods 0.000 abstract description 13
- 238000010586 diagram Methods 0.000 description 5
- 241000208340 Araliaceae Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 238000000701 chemical imaging Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000002595 magnetic resonance imaging Methods 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 238000012634 optical imaging Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000002603 single-photon emission computed tomography Methods 0.000 description 1
- 238000003325 tomography Methods 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/0059—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence
- A61B5/0071—Measuring for diagnostic purposes; Identification of persons using light, e.g. diagnosis by transillumination, diascopy, fluorescence by measuring fluorescence emission
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/72—Signal processing specially adapted for physiological signals or for diagnostic purposes
- A61B5/7235—Details of waveform analysis
- A61B5/7253—Details of waveform analysis characterised by using transforms
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Abstract
The invention provides a kind of fluoroscopic imaging device and method, device includes：Light source module, optical signalling acquisition module and processing module, light source module include：LASER Light Source and visible light source, exciting light and visible ray are provided for search coverage；Optical signalling acquisition module includes：Camera lens, Amici prism, speculum, the first CCD camera and the second CCD camera, the end of camera lens is connected with the incidence end of Amici prism, one exit end of Amici prism is connected to the first CCD camera, and another exit end of Amici prism is connected to the second CCD camera by speculum；The reflected light of search coverage is transmitted to Amici prism by camera lens, and the image that the first CCD camera and the second CCD camera are gathered by Amici prism and speculum respectively, processing module generates fluorescence imaging result according to the first CCD camera and the 2nd the CCD image gathered.Double spectrum can be imaged simultaneously by the present invention and image co-registration, obtains more fluorescence informations, extends the scope of the research of optical molecular image and application.
Description
Technical field
The present invention relates to optical image technology, is concretely a kind of fluoroscopic imaging device and method.
Background technology
After radionuclide imaging, positron emission computerized tomography, single photon emission computed tomography and magnetic resonance imaging
Afterwards, in recent years, molecular imaging technology continues to develop, one of important mode as molecular image, optical molecular image skill
Art is increasingly becoming study hotspot, and wherein near-infrared fluorescence imaging receives much attention.
The fluoroscopic imaging systems of prior art are the monocular imaging system for single wavelength mostly, and the method utilized is to be directed to
The single wave band of dynamic process, or interest wave band detect one by one, can not be directed to multiple wave bands and obtain simultaneously and to image
Fusion treatment is carried out, i.e., fluorescent products most on the market are imaged using monocular imaging system at present, and its shortcoming exists
Fluoroscopic image or visible images are can only see when imaging, and multispectral image can not be seen.
The content of the invention
To reduce the threshold of optical molecular imaging research, the scope of the research of optical molecular image and application has been expanded, this
Inventive embodiments provide a kind of fluoroscopic imaging device, including：Light source module, optical signalling acquisition module and processing module,
Wherein,
Light source module includes：LASER Light Source and visible light source, for providing exciting light and visible ray for search coverage；
Optical signalling acquisition module includes：Camera lens, Amici prism, speculum, the first CCD camera and the second CCD camera,
The end of camera lens is connected with the incidence end of Amici prism, and an exit end of Amici prism is connected to the first CCD camera, light splitting
Another exit end of prism is connected to the second CCD camera by speculum；
The reflected light of search coverage is transmitted to Amici prism by camera lens, and the first CCD camera and the second CCD camera are led to respectively
Amici prism and the image of speculum collection are crossed, the processing module is given birth to according to the first CCD camera and the 2nd the CCD image gathered
Into fluorescence imaging result.
In the embodiment of the present invention, light source module also includes：First optical filter and the second optical filter；
First optical filter is connected to the LASER Light Source by laser fiber；
Second optical filter is connected to the visible light source by visible ray optical fiber.
In the embodiment of the present invention, the spectral coverage of the first optical filter is 710nm-770nm, a diameter of 25mm；Second optical filter
Spectral coverage is 400nm-650nm, a diameter of 25mm.
In the embodiment of the present invention, light source module optical signalling acquisition module also includes：3rd optical filter and the 4th optical filter；
One exit end of Amici prism is connected to the first CCD camera by the 3rd optical filter；
Speculum is connected to the second CCD camera by the 4th optical filter.
In the embodiment of the present invention, the spectral coverage of the 3rd optical filter is 400nm-650nm, a diameter of 25mm；4th optical filter
Spectral coverage is 810nm-870nm, a diameter of 25mm.
In the embodiment of the present invention, described visible light source is halogen light light source.
Meanwhile the present invention also provides a kind of fluorescence imaging method, fluorescence imaging is generated using above-mentioned fluoroscopic imaging device
As a result.
In the embodiment of the present invention in fluorescence imaging method, processing module gathers to the first CCD camera and the second CCD camera
Image carries out fusion treatment, using the fused images of generation as fluorescence imaging result.
In the embodiment of the present invention, the image that processing module gathers according to the first CCD camera and the second CCD camera generates fluorescence
Imaging results include：
Wavelet decomposition is carried out respectively to two images of two CCD cameras collection, generates the low frequency component and high frequency of original image
Component, wherein, the high fdrequency component includes：Horizontal high frequency component, vertical high frequency component and diagonal high fdrequency component；
The low frequency components of two images is weighted average, determines the wavelet coefficient of fused images low frequency part；
Compare the wavelet coefficient absolute value of the high fdrequency component of two images, scheme the high fdrequency component of maximum absolute value as fusion
As the wavelet coefficient of HFS；
Two picture centre regions are chosen with default size, the mean variance of pixel in chosen area are calculated, by mean variance
Wavelet coefficient of the wavelet coefficient of maximum image as fused images katolysis layer；
According to the wavelet coefficient of the low frequency part of the fused images of determination, the wavelet coefficient and katolysis of HFS
The wavelet coefficient of layer carries out wavelet inverse transformation generation fused images, using the fused images of generation as fluorescence imaging result.
In the embodiment of the present invention, the central area of two images is chosen with default line number M and columns N, wherein, M=3, N=
3。
The present invention is excited by light source module to search coverage, carries out collection light in real time, optical filter module is not to
Light with spectral coverage is filtered, and the image information collected is handled in real time, the image co-registration of different spectral coverage is arrived
Together, realize the image co-registration of spectrum and shown, fluorescence imaging is composed using binocular bifocal, double spectrum can be carried out simultaneously
Imaging and image co-registration, obtain more fluorescence informations, have broken technical monopoly situation of the offshore company in China, have extended optics point
Sub-image studies the scope with application.
For the above and other objects, features and advantages of the present invention can be become apparent, preferred embodiment cited below particularly,
And coordinate institute's accompanying drawings, it is described in detail below.
Brief description of the drawings
In order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is the required accompanying drawing used in technology description to be briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, on the premise of not paying creative work, can be with
Other accompanying drawings are obtained according to these accompanying drawings.
Fig. 1 discloses a kind of block diagram of fluoroscopic imaging device for the present invention；
Fig. 2 is the block diagram of fluoroscopic imaging device in an embodiment of the present invention；
Fig. 3 discloses a kind of fluorescence imaging method flow chart for the present invention；
Fig. 4 is the structured flowchart that the embodiment of the present invention composes fluoroscopic imaging systems based on binocular bifocal；
Fig. 5 is the schematic diagram that the embodiment of the present invention composes fluoroscopic imaging systems based on binocular bifocal；
Fig. 6 is the principle of image fusion figure based on wavelet transformation in the present embodiment.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
The present invention discloses a kind of fluoroscopic imaging device, as shown in figure 1, the device includes：Light source module 101, optical signalling
Acquisition module 102 and processing module 103, wherein,
Light source module includes：LASER Light Source 1011 and visible light source 1012, for for search coverage provide exciting light and
Visible ray；
Optical signalling acquisition module includes：Camera lens 1021, Amici prism 1022, speculum 1023, the first CCD camera 1204
And the 2nd CCD1025 camera, the end of camera lens 1021 are connected with the incidence end of Amici prism 1022, Amici prism 1022
One exit end is connected to the first CCD camera 1024, and another exit end of Amici prism 1022 is connected by speculum 1023
To the second CCD camera 1025；
The reflected light of search coverage is transmitted to Amici prism 1022, the first CCD camera 1024 and second by camera lens 1021
The image that CCD camera 1025 is gathered by Amici prism 1022 and speculum 1023 respectively, processing module 103 is according to the first CCD
The image generation fluorescence imaging result of the CCD1025 of camera 1024 and the 2nd collections.
Meanwhile as shown in Fig. 2 in an embodiment of the present invention, light source module also includes：First optical filter 1013 and second
Optical filter 1014；
First optical filter 1013 is connected to LASER Light Source 1011 by laser fiber；
Second optical filter 1014 is connected to visible light source 1012 by visible ray optical fiber.
Light source module optical signalling acquisition module also includes：3rd optical filter 1026 and the 4th optical filter 1027；
One exit end of Amici prism 1022 is connected to the first CCD camera 1027 by the 3rd optical filter 1026；
Speculum 1023 is connected to the second CCD camera 1024 by the 4th optical filter 1027.
Meanwhile invention additionally discloses a kind of fluorescence imaging method, fluorescence imaging is generated using foregoing fluoroscopic imaging device
As a result, the image that processing module gathers to the first CCD camera 1024 and the second CCD camera 1025 carries out fusion treatment, will generate
Fused images as fluorescence imaging result.
In the embodiment of the present invention, the image that processing module gathers according to the first CCD camera and the second CCD camera generates fluorescence
Imaging results, as shown in figure 3, specifically including：
Step S301, wavelet decomposition is carried out respectively to two images of two CCD cameras collection, generates the low frequency point of original image
Amount and high fdrequency component, wherein, the high fdrequency component includes：Horizontal high frequency component, vertical high frequency component and diagonal high fdrequency component；
Step S302, is weighted average to the low frequency components of two images, determines the wavelet systems of fused images low frequency part
Number；
Step S303, compare the wavelet coefficient absolute value of the high fdrequency component of two images, by the high fdrequency component of maximum absolute value
Wavelet coefficient as fused images HFS；
Step S304, two picture centre regions are chosen with default size, calculate the mean variance of pixel in chosen area, will
Wavelet coefficient of the wavelet coefficient of the maximum image of mean variance as fused images katolysis layer；
Step S305, according to the wavelet coefficient of the wavelet coefficient of the low frequency part of the fused images of determination, HFS with
And the wavelet coefficient of katolysis layer carries out wavelet inverse transformation generation fused images, using the fused images of generation as fluorescence imaging
As a result.
Technical scheme is described in further details with reference to specific embodiment.
It is proposed that a kind of binocular bifocal that is based on composes fluoroscopic imaging systems in the present embodiment, Fig. 4 is the embodiment of the present invention based on double
The structured flowchart of the double spectrum fluoroscopic imaging systems of mesh, Fig. 5 are that the embodiment of the present invention composes fluoroscopic imaging systems based on binocular bifocal
Schematic diagram.
As illustrated, the binocular bifocal spectrum fluoroscopic imaging systems of the present embodiment include：Light source module 110, optical signalling are adopted
Collect module 120, computer control and processing module 130 and optical filter module 140, wherein：
Light source module 110, for being irradiated to the search coverage 100 of test serum, provide and excite for search coverage 100
Light and visible ray；
Optical signalling acquisition module 120, for obtaining fluorescence and visible images according to the reflected light of search coverage 100；
Computer control be connected with processing module 130 with optical signalling acquisition module 120 by data wire 101, for pair
CCD camera 124 and CCD camera 127 in the optical signalling acquisition module 120 are controlled, to optical signalling acquisition module
120 fluorescence collected and visible images carry out fusion treatment and shown；
Optical filter module 140, for providing the filter of different spectrum for light source module 110 and optical signalling acquisition module 120
Mating plate.
Light source module 110 further comprises：Exciting light optical fiber 115, visible ray optical fiber 116, optical filter 112, optical filter
114th, excitation source 113 and visible light source 111, wherein, exciting light optical fiber is connected with optical filter 112, for being guided out exciting light
The exciting light that light source 113 is sent, to carry out exciting light irradiation to search coverage 100；Visible ray optical fiber is connected with optical filter 114,
The visible ray sent for being guided out the visible light source 111, lighting source is provided for search coverage 100；
Optical signalling acquisition module 120 further comprise camera lens 121, Amici prism 122, speculum 125, optical filter 123,
Optical filter 126, CCD camera 124, CCD camera 127, wherein, Amici prism 122 is made up of 55 Amici prisms, Amici prism 122
Incident light end be connected with the end of camera lens 122, two exit ends connect optical filter 123 and speculum 125, optical filter respectively
124 with CCD camera 124) be connected, the incident light end of speculum 125 is connected with an exit end of Amici prism 122, exit end
It is connected by optical filter 126 with the CCD camera 127, the Ray Of Light for camera lens 122 to be transmitted is divided into two beams；CCD camera
124) control with computer and be connected with processing module 130 with CCD camera 127, for according to Amici prism 122 and speculum 125
Emergent ray be imaged, and there is the image transmitting of different spectrum or different-energy to the calculating by what is respectively obtained
Machine controls and processing module 130.
The relative distance between each device in optical signalling acquisition module 120 is fixed, i.e., optical signal passes through mirror
Head enters in the system, is first converted into directional light, light is divided into two by Amici prism 122 and speculum 125
Beam, it is imaged respectively by two CCD cameras.So the optical signalling acquisition module 120 is a general light splitting mould
Block, i.e., no matter camera lens selection is endoscope, C interface camera lens or F interface camera lens, can as long as being adjusted to suitable flange distance
Blur-free imaging on computers.
In the present embodiment, computer control further comprises control module 131, image processing module with processing module 130
132 and display module 133, wherein, the control module 131 is used for the imaging ginseng to the CCD camera 124 and CCD camera 127
Number (such as time for exposure etc.) is controlled；Image processing module 132 is used to shoot CCD camera 124 and CCD camera 127
To view data handled, processing include the functions such as image co-registration；Wherein, it is to be become based on small echo to handle Image Fusion
The Image Fusion changed, it is the Image Fusion schematic diagram of the invention based on wavelet transformation as shown in Figure 6, specific steps are such as
Under：
Step 1, the image A that CCD camera 124 the collects and image B that CCD camera 127 collects is subjected to wavelet decomposition：
A in formulaj+1,The low frequency component of expression original image respectively, horizontal high frequency component, vertically
High fdrequency component and diagonal high fdrequency component, h and g represent the low pass and high-pass filtering operator of image respectively, and its subscript represents current point
Solve the row and column of the image of layer, aj(l, k) represents the low frequency component of a decomposition layer on image；Z, K is image-region.
Step 2, fused images F low frequency part is determined, takes the low frequency component weighted average after source images A, B decomposition, i.e.,
CN,F=(CN,A+CN,B)/2
Wherein, CN,A、CN,BThe low frequency component of source images A, B on wavelet decomposition scales, C are represented respectivelyN,FExpression will determine
Low frequency components of the fused images F on wavelet decomposition scales；
Step 3, on highest decomposition layer, compare the wavelet coefficient of 3 direction high fdrequency components of A, B image, take absolute value
Wavelet coefficient of the big wavelet coefficient as fused images F；
Step 4, on katolysis layer, the regional area centered on capture element (takes the office of 3 × 3 sizes in the present embodiment
Portion region) maximum the image A or B of mean variance wavelet coefficient determine as wavelet coefficient corresponding to fused images F, its variance
Justice：
Wherein, M, N are respectively the line number and columns (being here 3) of regional area, xi,jFor one in current regional area
Grey scale pixel value,For the average value of current regional area grey scale pixel value；
Step 5, after each wavelet coefficient for determining fused images F, i.e., it is determined that fused images F low frequency component wavelet systems
After number, highest decomposition layer wavelet coefficient and katolysis layer wavelet coefficient, inverse wavelet transform is carried out, that is, obtains fused images F.
Step 6, export and show fused images F.
The principle of image fusion figure based on wavelet transformation of the present embodiment is as shown in Figure 6.
In the present embodiment, display module 133 is used to carry out in real time for the image obtained after the processing of image processing module 132
It has been shown that, observed for operating personnel.
Optical filter module 140 is used for the exciting characteristic according to different fluorescence, adjusts the spectral coverage of each optical filter, double to ensure
Exciting and gathering for spectrum light, avoids interfering for different spectrum lights.The spectral coverage of each optical filter once adjusted,
To no longer it switch during whole real time imagery.The quantity of the optical filter can be installed as needed, in the present invention
In one embodiment, the quantity of the optical filter is 4：Optical filter 112, optical filter 114, optical filter 123 and optical filter 4 12, institute
The spectral coverage for stating optical filter is near infrared range, is specially：
The spectral coverage of optical filter 112 is 710nm-770nm, a diameter of 25mm；
The spectral coverage of optical filter 114 is 400nm-650nm, a diameter of 25mm；
The spectral coverage of optical filter 123 is 400nm-650nm, a diameter of 25mm；
The spectral coverage of optical filter 126 is 810nm-870nm, a diameter of 25mm；
In operating personnel in actual use, the optical filter with suitable spectrum can be switched according to specific demand,
It is not limited with the scope described in the embodiment of the present invention.
Using the method that the multi-optical spectrum imaging system based on endoscope carries out multispectral imaging in the embodiment of the present invention, it has
Body comprises the following steps：
Step S1, exciting light sources 111 and visible light source 113 is set to be irradiated search coverage 100 respectively；
Step S2, according to detection feature, optical filter module 150 is for light source module 110, optical signalling acquisition module
(120) optical filter is configured in；
Step S3, control module 131 are adjusted to the imaging parameters of CCD camera 124 and CCD camera 127, the CCD
The reflection light collection that camera 124 and CCD camera 127 have different spectrum or energy according to the search coverage 100 respectively obtains
To image；
Step S4, image processing module 132 is to the CCD camera 124) and the image that collects of CCD camera 127 carry out
Image co-registration processing；
Step S5, the image after the processing that display module 133 obtains for the step S4 carries out real-time display, if display
Image do not reach definition requirement, then the parameter of endoscope head 122 is adjusted by optical signalling acquisition module 120, until
The image that the display module 133 is shown reaches definition requirement.
The present invention uses according to the characteristics of optical molecular image, and based on for a long time in the research experience of optical imaging field
Two CCD cameras realize the functions such as the acquisition of fluorescence, visible ray and split image.And provide a kind of general imaging system
System, i.e., no matter camera lens selection is endoscope, C interface camera lens or F interface camera lens, can as long as being adjusted to suitable flange distance
Blur-free imaging on computers, observed for staff.
The present invention is excited by light source module to search coverage, and optical signalling acquisition module carries out collection light in real time
Line, optical filter module filter to the light of different spectral coverage, and computer module carries out real-time to the image information collected
Processing, the image co-registration of different spectral coverage to the image co-registration for together, realizing spectrum and is shown.Current big portion exhausted on the market
Point fluorescent product is imaged using monocular imaging system, and its shortcoming is to can only see fluoroscopic image or visible when being imaged
Light image, and multispectral image can not be seen.And the present invention effectively solves the problem, while offshore company is also broken
In the technical monopoly situation of China, the threshold of optical molecular imaging research is reduced, has expanded the research of optical molecular image and application
Scope.
Apply specific embodiment in the present invention to be set forth the principle and embodiment of the present invention, above example
Explanation be only intended to help understand the present invention method and its core concept；Meanwhile for those of ordinary skill in the art,
According to the thought of the present invention, there will be changes in specific embodiments and applications, in summary, in this specification
Appearance should not be construed as limiting the invention.
Claims (10)
1. a kind of fluoroscopic imaging device, it is characterised in that described device includes：Light source module, optical signalling acquisition module with
And processing module, wherein,
Described light source module includes：LASER Light Source and visible light source, exciting light and visible ray are provided for search coverage；
Described optical signalling acquisition module includes：Camera lens, Amici prism, speculum, the first CCD camera and the 2nd CCD phases
Machine, the end of camera lens are connected with the incidence end of Amici prism, and an exit end of Amici prism is connected to the first CCD camera,
Another exit end of Amici prism is connected to the second CCD camera by speculum；
The reflected light of search coverage is transmitted to Amici prism by camera lens, and the first CCD camera and the second CCD camera are respectively by dividing
Light prism and the image of speculum collection, the image generation that the processing module gathers according to the first CCD camera and the 2nd CCD are glimmering
Photoimaging result.
2. fluoroscopic imaging device as claimed in claim 1, it is characterised in that described light source module also includes：First filters
Piece and the second optical filter；
First optical filter is connected to the LASER Light Source by laser fiber；
Second optical filter is connected to the visible light source by visible ray optical fiber.
3. fluoroscopic imaging device as claimed in claim 2, it is characterised in that
The spectral coverage of the first described optical filter is 710nm-770nm, a diameter of 25mm；
The spectral coverage of the second described optical filter is 400nm-650nm, a diameter of 25mm.
4. fluoroscopic imaging device as claimed in claim 1, it is characterised in that described light source module optical signalling acquisition module
Also include：3rd optical filter and the 4th optical filter；
One exit end of described Amici prism is connected to the first CCD camera by the 3rd optical filter；
Described speculum is connected to the second CCD camera by the 4th optical filter.
5. fluoroscopic imaging device as claimed in claim 4, it is characterised in that
The spectral coverage of the 3rd described optical filter is 400nm-650nm, a diameter of 25mm；
The spectral coverage of the 4th described optical filter is 810nm-870nm, a diameter of 25mm.
6. fluoroscopic imaging device as claimed in claim 1, it is characterised in that described visible light source is halogen light light
Source.
7. a kind of fluorescence imaging method, it is characterised in that described method utilizes the fluoroscopic imaging device described in claim 1-4
Generate fluorescence imaging result.
8. fluorescence imaging method as claimed in claim 7, it is characterised in that the processing module according to the first CCD camera and
The image generation fluorescence imaging result of 2nd CCD collections includes：
The image that processing module gathers to the first CCD camera and the second CCD camera carries out fusion treatment, by the fused images of generation
As fluorescence imaging result.
9. fluorescence imaging method as claimed in claim 7, it is characterised in that the processing module according to the first CCD camera and
The image generation fluorescence imaging result of second CCD camera collection includes：
Wavelet decomposition is carried out respectively to two images of two CCD cameras collection, generates the low frequency component and high fdrequency component of original image,
Wherein, the high fdrequency component includes：Horizontal high frequency component, vertical high frequency component and diagonal high fdrequency component；
The low frequency components of two images is weighted average, determines the wavelet coefficient of fused images low frequency part；
Compare the wavelet coefficient absolute value of the high fdrequency component of two images, it is high using the high fdrequency component of maximum absolute value as fused images
The wavelet coefficient of frequency part；
Two picture centre regions are chosen with default size, calculate the mean variance of pixel in chosen area, mean variance is maximum
Image wavelet coefficient of the wavelet coefficient as fused images katolysis layer；
According to the wavelet coefficient of the low frequency part of the fused images of determination, the wavelet coefficient of HFS and katolysis layer
Wavelet coefficient carries out wavelet inverse transformation generation fused images, using the fused images of generation as fluorescence imaging result.
10. fluorescence imaging method as claimed in claim 9, it is characterised in that described that two picture centres are chosen with default size
Region includes：The central area of two images is chosen with default line number M and columns N, wherein, M=3, N=3.
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| CN109171661A (en) * | 2018-10-10 | 2019-01-11 | 南京诺源医疗器械有限公司 | Fluoroscopic visualization checks system excitation light source |
| CN110893095A (en) * | 2018-09-12 | 2020-03-20 | 上海逸思医学影像设备有限公司 | System and method for visible light and excited fluorescence real-time imaging |
| CN111988533A (en) * | 2019-05-23 | 2020-11-24 | 川田科技株式会社 | Welding assisting method and device |
| CN114731364A (en) * | 2019-10-02 | 2022-07-08 | 化学影像公司 | Fusion of molecular chemical imaging with RGB imaging |
| CN115480385A (en) * | 2021-05-31 | 2022-12-16 | 博瑞生物医疗科技(深圳)有限公司 | Surgical microscope system and imaging method thereof |
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