CN104299212A - Method and system for fusing white-light image and fluorescence image - Google Patents
Method and system for fusing white-light image and fluorescence image Download PDFInfo
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- CN104299212A CN104299212A CN201410510051.9A CN201410510051A CN104299212A CN 104299212 A CN104299212 A CN 104299212A CN 201410510051 A CN201410510051 A CN 201410510051A CN 104299212 A CN104299212 A CN 104299212A
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Abstract
The invention discloses a method and system for fusing a white-light image and a fluorescence image. The method for fusing the white-light image and the fluorescence image comprises the steps that S101, the fluorescence image is enhanced, so that an enhanced fluorescence image is obtained; S102, pseudo color processing is conducted on the enhanced fluorescence image, so that a pseudo color fluorescence image is obtained; S103, the pseudo color fluorescence image and the white-light image are fused, so that a fused image is obtained. By the adoption of the method and system for fusing the white-light image and the fluorescence image, the problem that in the prior art, well real-time imaging conducted by fusing a white-light image and a fluorescence image is not available is solved.
Description
Technical field
The present invention relates to image co-registration, particularly, relate to fusion method and the system of a kind of White-light image and fluoroscopic image.
Background technology
In existing technology, fluorescence and White-light image are generally separately checked, in fluorescence excitation imaging technique, the macroscopic light beam that White-light image is behaved the image of photograph, fluorescence excitation is the excitation source by vitro, fluorophor in irradiating biological body, it is made to reach higher-energy state, fluorophor absorbs luminous energy electronic transition is arrived excited state, electronics is got back to the process of ground state can discharge fluorescence from excited state, fluorescence is propagated in organizer, a part passes arrival body surface, detected by fluorescent probe thus can fluoroscopic image be formed, so existing technology is that fluorescence and White-light image are separately checked, so both inconvenience can not well merge and real time imagery fluorescence and white light.
Summary of the invention
The object of the invention is to overcome the not fine problem carrying out White-light image and fluoroscopic image to merge real time imagery in prior art, a kind of method and system that conveniently fluorescence and white light can be carried out merging is provided.
The invention provides a kind of fusion method of image, this fusion method comprises:
S101, carries out enhancing and to be enhanced fluoroscopic image by fluoroscopic image;
S102, carries out Pseudo Col ored Image by described enhancing fluoroscopic image and obtains pseudo-colours fluoroscopic image;
S103, carries out fusion by described pseudo-colours fluoroscopic image and White-light image and obtains fused images.
Preferably, step S101 comprises:
S211, carries out wavelet transformation by described fluoroscopic image and obtains scale coefficient and wavelet coefficient;
S221, carries out low-frequency information and strengthens and obtain described low-frequency information and strengthen signal by described scale coefficient, and described wavelet coefficient is carried out high-frequency information denoising and strengthen and obtain described high-frequency information denoising and strengthen signal again;
S231, strengthens signal and described high-frequency information denoising and strengthens signal again and carry out wavelet inverse transformation and to be enhanced fluoroscopic image by described low-frequency information.
Preferably, step S102 comprises:
S212, the large young pathbreaker's gray level according to gray level is divided into 8 intervals, and described 8 intervals are respectively 8 kinds of different colors;
S222, described enhancing fluoroscopic image is carried out Pseudo Col ored Image by the gray level according to described enhancing fluoroscopic image.
Preferably, described 8 intervals comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, gray level is the 3rd interval of 64-95 level, gray level is the 4th interval of 96-127 level, and gray level is the 5th interval of 128-159 level, and gray level is the 6th interval of 160-191 level, gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
Preferably, described step S102 comprises:
S312, carries out fast fourier transform to described enhancing fluoroscopic image;
S322, obtains image after low-pass filtering to carrying out low-pass filtering through the fluoroscopic image of Fourier transform,
Image after bandpass filtering is obtained to carrying out bandpass filtering through the fluoroscopic image of Fourier transform, and
Image after high-pass filtering is obtained to carrying out high-pass filtering through the fluoroscopic image of Fourier transform;
S332, carries out Fourier inversion respectively by image after image after image, described bandpass filtering after described low-pass filtering and described high-pass filtering and obtains pseudo-colours fluoroscopic image;
The present invention also provides the emerging system of a kind of White-light image and fluoroscopic image, and this emerging system comprises:
Fluoroscopic image is carried out the equipment strengthening the fluoroscopic image that is enhanced;
Described enhancing fluoroscopic image is carried out the equipment that Pseudo Col ored Image obtains pseudo-colours fluoroscopic image;
Described pseudo-colours fluoroscopic image and White-light image are carried out merging the equipment obtaining fused images.
Preferably, this emerging system also comprises:
Raw fluorescence image is carried out the equipment that wavelet transformation obtains scale coefficient and wavelet coefficient;
Described scale coefficient is carried out low-frequency information and strengthen the equipment obtaining described low-frequency information enhancing signal;
Described wavelet coefficient is carried out high-frequency information denoising to strengthen again and obtain the equipment that described high-frequency information denoising strengthens signal again;
Described low-frequency information is strengthened signal and described high-frequency information denoising to strengthen signal again and carry out wavelet inverse transformation and to be enhanced the equipment of fluoroscopic image.
Preferably, this emerging system also comprises:
Be divided into 8 interval equipment according to large young pathbreaker's gray level of gray level, described 8 intervals are respectively 8 kinds of different colors;
According to the gray level of described enhancing fluoroscopic image, described enhancing fluoroscopic image is carried out the equipment of Pseudo Col ored Image.
Preferably, described 8 intervals comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, gray level is the 3rd interval of 64-95 level, gray level is the 4th interval of 96-127 level, and gray level is the 5th interval of 128-159 level, and gray level is the 6th interval of 160-191 level, gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
Preferably, this system also comprises:
Fluoroscopic image after enhancing is carried out to the equipment of fast fourier transform;
To the equipment carrying out low-pass filtering through the fluoroscopic image of Fourier transform and obtain image after low-pass filtering; To the equipment carrying out bandpass filtering through the fluoroscopic image of Fourier transform and obtain image after bandpass filtering; And to carrying out high-pass filtering through the fluoroscopic image of Fourier transform and obtain the equipment of image after high-pass filtering;
Image graph picture after image after image, described bandpass filtering after described low-pass filtering and described high-pass filtering is carried out the equipment that Fourier inversion obtains pseudo-colours fluoroscopic image respectively.
By above-mentioned embodiment, the fusion method of a kind of White-light image of the present invention and fluoroscopic image overcomes the not fine problem carrying out White-light image and fluoroscopic image to merge real time imagery in prior art, there is provided a kind of method conveniently fluorescence and white light can being carried out merging, by strengthening fluoroscopic image, increasing to strengthening image the fusion that pseudo-colours finally realizes pseudo-colours fluoroscopic image and original white light image.
Other features and advantages of the present invention are described in detail in embodiment part subsequently.
Accompanying drawing explanation
Accompanying drawing is used to provide a further understanding of the present invention, and forms a part for instructions, is used from explanation the present invention, but is not construed as limiting the invention with embodiment one below.In the accompanying drawings:
Fig. 1 is the process flow diagram of the fusion method that a kind of White-light image of the present invention and fluoroscopic image are described.
Embodiment
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail.Should be understood that, embodiment described herein, only for instruction and explanation of the present invention, is not limited to the present invention.
In the embodiment of the fusion method of a kind of White-light image of the present invention and fluoroscopic image, this fusion method comprises:
S101, carries out enhancing and to be enhanced fluoroscopic image by original fluoroscopic image data;
S102, carries out Pseudo Col ored Image by enhancing fluoroscopic image and obtains pseudo-colours fluoroscopic image;
S103, carries out fusion by pseudo-colours fluoroscopic image and original white light image and obtains fused images.
By above-mentioned embodiment, the fusion method of a kind of White-light image of the present invention and fluoroscopic image overcomes the not fine problem carrying out White-light image and fluoroscopic image to merge real time imagery in prior art, a kind of method conveniently fluorescence and white light can being carried out merging is provided, by strengthening fluoroscopic image, increasing to strengthening image the fusion that pseudo-colours finally realizes pseudo-colours fluoroscopic image and original white light image, strengthening image is in order to the denoising signal useful with reinforcement, pseudo-colours is that conveniently observer observes, and increases the sharpness of image.
Be described further below in conjunction with the fusion method of accompanying drawing 1 to White-light image of the present invention and fluoroscopic image, in the method, S101 is realized by small wave converting method, and S102 realizes by gray level classification or by frequency domain Pseudo Col ored Image.
In the embodiment of the fusion method of a kind of White-light image of the present invention and fluoroscopic image, in step S101,
S211, carries out wavelet transformation by Raw fluorescence image and obtains scale coefficient and wavelet coefficient;
S221, carries out low-frequency information enhancing and obtains described low-frequency information enhancing signal by described scale coefficient;
Described wavelet coefficient is carried out high-frequency information denoising to strengthen again and obtain described high-frequency information denoising and strengthen signal again;
S231, strengthens signal and described high-frequency information denoising and strengthens signal again and carry out wavelet inverse transformation and to be enhanced fluoroscopic image by described low-frequency information.
By above-mentioned embodiment, the enhancing of fluoroscopic image can being realized, by strengthening while scale coefficient and wavelet coefficient, and during this period denoising having been carried out to high frequency imaging.
In this kind of embodiment, step S102 comprises:
S212, can be divided into 8 intervals according to large young pathbreaker's gray level of gray level, and described 8 intervals are respectively 8 kinds of different colors;
S222, described enhancing fluoroscopic image is carried out Pseudo Col ored Image by the gray level according to described enhancing fluoroscopic image.
Present embodiment carries out Pseudo Col ored Image by gray scale top and bottom process, if the gray-scale value of original black white image can represent with a functional value, it converts in certain interval, the different colour of different gray scales is realized by this kind of mode, realize the object of enameling to black white image, mode of operation is simple, successful.
In this kind of embodiment, in step S212, preferably gray level can be divided into following 8 intervals according to size, comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, gray level is the 3rd interval of 64-95 level, gray level is the 4th interval of 96-127 level, and gray level is the 5th interval of 128-159 level, and gray level is the 6th interval of 160-191 level, gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
In the another kind of embodiment of the fusion method of a kind of White-light image of the present invention and fluoroscopic image, in step s 102,
S312, carries out fast fourier transform to described enhancing fluoroscopic image;
S322, obtains image after low-pass filtering to carrying out low-pass filtering through the fluoroscopic image of Fourier transform;
Image after bandpass filtering is obtained to carrying out bandpass filtering through the fluoroscopic image of Fourier transform; And
Image after low-pass filtering is obtained to carrying out low-pass filtering through the fluoroscopic image of Fourier transform;
S332, image after image after image, described bandpass filtering after described low-pass filtering and described low-pass filtering is carried out Fourier inversion respectively and obtains pseudo-colours fluoroscopic image, wherein, pseudo-colours fluoroscopic image can be obtained by additional treatments after inverse fourier transform;
This kind of embodiment is the Pseudo Col ored Image of spatial frequency domain, the fluoroscopic image of original black and white is divided into three kinds of different wave filters to carry out filtering and finally form pseudo color image by the fluoroscopic image frequency distribution of original black and white as red, green, blue three kinds of primary colours of color-output device, wherein additional treatments comprises the methods such as histogram equalization.
The present invention also provides the emerging system of a kind of White-light image and fluoroscopic image, and this emerging system comprises:
Fluoroscopic image is carried out the equipment strengthening the fluoroscopic image that is enhanced;
Described enhancing fluoroscopic image is carried out the equipment that Pseudo Col ored Image obtains pseudo-colours fluoroscopic image;
Described pseudo-colours fluoroscopic image and White-light image are carried out merging the equipment obtaining fused images.
In this kind of embodiment, Raw fluorescence image is carried out the equipment that wavelet transformation obtains scale coefficient and wavelet coefficient;
Described scale coefficient is carried out low-frequency information and strengthen the equipment obtaining described low-frequency information enhancing signal;
Described wavelet coefficient is carried out high-frequency information denoising to strengthen again and obtain the equipment that described high-frequency information denoising strengthens signal again;
Described low-frequency information is strengthened signal and described high-frequency information denoising to strengthen signal again and carry out wavelet inverse transformation and to be enhanced the equipment of fluoroscopic image.
In the preferred implementation of the emerging system of a kind of White-light image of the present invention and fluoroscopic image, this system also comprises:
Be divided into 8 interval equipment according to large young pathbreaker's gray level of gray level, described 8 intervals are respectively 8 kinds of different colors;
According to the gray level of described enhancing fluoroscopic image, described enhancing fluoroscopic image is carried out the equipment of Pseudo Col ored Image.
In this kind of embodiment, described 8 intervals comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, gray level is the 3rd interval of 64-95 level, gray level is the 4th interval of 96-127 level, and gray level is the 5th interval of 128-159 level, and gray level is the 6th interval of 160-191 level, gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
In this kind of embodiment, by the equipment of the fluoroscopic image after enhancing through fast fourier transform;
This system also comprises:
Fluoroscopic image after enhancing is carried out to the equipment of fast fourier transform;
To the equipment carrying out low-pass filtering through the fluoroscopic image of Fourier transform and obtain image after low-pass filtering; To the equipment carrying out bandpass filtering through the fluoroscopic image of Fourier transform and obtain image after bandpass filtering; And to carrying out high-pass filtering through the fluoroscopic image of Fourier transform and obtain the equipment of image after high-pass filtering;
Image graph picture after image after image, described bandpass filtering after described low-pass filtering and described high-pass filtering is carried out the equipment that Fourier inversion obtains pseudo-colours fluoroscopic image respectively.
In sum, the fusion method of a kind of White-light image of the present invention and fluoroscopic image and and system overcoming the not good problem carrying out White-light image and fluoroscopic image to merge real time imagery in prior art, only need when observation just both can see the specific features that White-light image is reacted by an image, also the specific features that fluoroscopic image reacts can be seen, observer observes easily, and achieve pseudo-colours on black and white fluoroscopic image by the Pseudo Col ored Image method of gray scale top and bottom process and spatial frequency domain, more convenient and practical, improve the efficiency of Pseudo Col ored Image.
Below the preferred embodiment of the present invention is described in detail by reference to the accompanying drawings; but; the present invention is not limited to the detail in above-mentioned embodiment; within the scope of technical conceive of the present invention; can carry out multiple simple variant to technical scheme of the present invention, these simple variant all belong to protection scope of the present invention.
It should be noted that in addition, each concrete technical characteristic described in above-mentioned embodiment, in reconcilable situation, can be combined by any suitable mode, in order to avoid unnecessary repetition, the present invention illustrates no longer separately to various possible array mode.
In addition, also can carry out combination in any between various different embodiment of the present invention, as long as it is without prejudice to thought of the present invention, it should be considered as content disclosed in this invention equally.
Claims (10)
1. a fusion method for image, is characterized in that, this fusion method comprises:
S101, carries out enhancing and to be enhanced fluoroscopic image by fluoroscopic image;
S102, carries out Pseudo Col ored Image by described enhancing fluoroscopic image and obtains pseudo-colours fluoroscopic image;
S103, carries out fusion by described pseudo-colours fluoroscopic image and White-light image and obtains fused images.
2. fusion method according to claim 1, is characterized in that, step S101 comprises:
S211, carries out wavelet transformation by described fluoroscopic image and obtains scale coefficient and wavelet coefficient;
S221, carries out low-frequency information and strengthens and obtain described low-frequency information and strengthen signal by described scale coefficient, and described wavelet coefficient is carried out high-frequency information denoising and strengthen and obtain described high-frequency information denoising and strengthen signal again;
S231, strengthens signal and described high-frequency information denoising and strengthens signal again and carry out wavelet inverse transformation and to be enhanced fluoroscopic image by described low-frequency information.
3. fusion method according to claim 1, it is characterized in that, step S102 comprises:
S212, the large young pathbreaker's gray level according to gray level is divided into 8 intervals, and described 8 intervals are respectively 8 kinds of different colors;
S222, described enhancing fluoroscopic image is carried out Pseudo Col ored Image by the gray level according to described enhancing fluoroscopic image.
4. fusion method according to claim 3, it is characterized in that, described 8 intervals comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, and gray level is the 3rd interval of 64-95 level, and gray level is the 4th interval of 96-127 level, gray level is the 5th interval of 128-159 level, gray level is the 6th interval of 160-191 level, and gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
5. fusion method according to claim 1, is characterized in that, described step S102 comprises:
S312, carries out fast fourier transform to described enhancing fluoroscopic image;
S322, obtains image after low-pass filtering to carrying out low-pass filtering through the fluoroscopic image of Fourier transform,
Image after bandpass filtering is obtained to carrying out bandpass filtering through the fluoroscopic image of Fourier transform, and
Image after high-pass filtering is obtained to carrying out high-pass filtering through the fluoroscopic image of Fourier transform; S332, carries out Fourier inversion respectively by image after image after image, described bandpass filtering after described low-pass filtering and described high-pass filtering and obtains pseudo-colours fluoroscopic image.
6. an emerging system for image, is characterized in that, this emerging system comprises:
Fluoroscopic image is carried out the equipment strengthening the fluoroscopic image that is enhanced;
Described enhancing fluoroscopic image is carried out the equipment that Pseudo Col ored Image obtains pseudo-colours fluoroscopic image;
Described pseudo-colours fluoroscopic image and White-light image are carried out merging the equipment obtaining fused images.
7. emerging system according to claim 6, is characterized in that, this emerging system also comprises:
Raw fluorescence image is carried out the equipment that wavelet transformation obtains scale coefficient and wavelet coefficient;
Described scale coefficient is carried out low-frequency information and strengthen the equipment obtaining described low-frequency information enhancing signal;
Described wavelet coefficient is carried out high-frequency information denoising to strengthen again and obtain the equipment that described high-frequency information denoising strengthens signal again;
Described low-frequency information is strengthened signal and described high-frequency information denoising to strengthen signal again and carry out wavelet inverse transformation and to be enhanced the equipment of fluoroscopic image.
8. according to the emerging system of claim 6 image, it is characterized in that, this emerging system also comprises:
Be divided into 8 interval equipment according to large young pathbreaker's gray level of gray level, described 8 intervals are respectively 8 kinds of different colors;
According to the gray level of described enhancing fluoroscopic image, described enhancing fluoroscopic image is carried out the equipment of Pseudo Col ored Image.
9. emerging system according to claim 8, it is characterized in that, described 8 intervals comprise: gray level is the first interval of 0-31 level, gray level is the second interval of 32-63 level, and gray level is the 3rd interval of 64-95 level, and gray level is the 4th interval of 96-127 level, gray level is the 5th interval of 128-159 level, gray level is the 6th interval of 160-191 level, and gray level is between the SECTOR-SEVEN of 192-223 level, and gray level is between the Section Eight of 224-225 level.
10. emerging system according to claim 6, is characterized in that, this emerging system also comprises:
Fluoroscopic image after enhancing is carried out to the equipment of fast fourier transform;
To the equipment carrying out low-pass filtering through the fluoroscopic image of Fourier transform and obtain image after low-pass filtering; To the equipment carrying out bandpass filtering through the fluoroscopic image of Fourier transform and obtain image after bandpass filtering; And to carrying out high-pass filtering through the fluoroscopic image of Fourier transform and obtain the equipment of image after high-pass filtering;
Image graph picture after image after image, described bandpass filtering after described low-pass filtering and described high-pass filtering is carried out the equipment that Fourier inversion obtains pseudo-colours fluoroscopic image respectively.
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Application publication date: 20150121 |