CN107513554A - A kind of food clean room environment microorganism-collecting method - Google Patents
A kind of food clean room environment microorganism-collecting method Download PDFInfo
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- CN107513554A CN107513554A CN201710677406.7A CN201710677406A CN107513554A CN 107513554 A CN107513554 A CN 107513554A CN 201710677406 A CN201710677406 A CN 201710677406A CN 107513554 A CN107513554 A CN 107513554A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
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- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
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Abstract
The invention discloses a kind of food clean room environment microorganism-collecting method, comprise the concrete steps that：First, surface microorganism is collected：(1), regular surfaces sample：Take 25cm2The position (clean bench surface) that samples on demand respectively of disposable TSA contact dish, time of contact is set to 10s；(2), irregular surface samples：Sampling is wiped in the operation room of excessive risk using the cotton swab of sterilizing, after the completion of insert in TSA culture mediums and cultivate, if any bacteria growing, line separation single bacterium colony；Contact dish after sampling is placed in preference temperature incubator and cultivated, it is stand-by；2nd, settling bacteria is collected：Collect routine work and the related settling bacteria in environmental monitoring control process；The present invention can effectively control the germ in food by collecting the microorganism in food toilet, improve food quality, ensure the safe handling of food.
Description
Technical field
The present invention relates to food technology field, specifically a kind of food clean room environment microorganism-collecting side
Method.
Background technology
Food security refer to it is nontoxic to food, harmless, meet due nutritional requirement, human body is not caused any acute
Or subacute and chronic hazard[1-2].Food security is related to planting, cultivates, processes, packing, storing, transporting, selling etc. many
Link.With the development of society and the raising of quality of life, the food-safe concern of people reaches unprecedented height.It is micro-
Biological pollution is the importance of current food problems, and current an important factor for causing death[3-5]。
The control of food microorganisms pathogenic bacteria is to improve food quality, ensure the important measures of food security.But in the modern times
Under the conditions of food production, randomness of the pollution with height of microorganism especially pathogenic bacteria[6].The positive of final test results
It is probably caused by sample itself carries pathogenic bacteria, it is also possible to be due to that experimenter or environmental pollution are drawn in checkout procedure
Rise.Therefore, establish the objectively assay accurate to offer of pathogenic bacteria detection bacterium tracing technology system in food, defended for food
Raw supervisory and management department's administration according to law and risk assessment tool are of great significance.
Significantly improved although modern food working condition and peopleware have, microorganism pollution is especially
Randomness of the pollution with height of pathogenic bacteria[7].Therefore, it is to carry out market surpervision to provide accurate, objective assay
It is essential with accurate risk assessment.
The content of the invention
In view of the defects and deficiencies of the prior art, the present invention intends to provide a kind of micro- life of food clean room environment
Thing collection method.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of food clean room environment microorganism-collecting method, is comprised the concrete steps that：
First, surface microorganism is collected：
(1), regular surfaces sample：Take 25cm2The position (clean bench that samples on demand respectively of disposable TSA contact dish
Surface), time of contact is set to 10s；
(2), irregular surface samples：Using sterilizing cotton swab excessive risk operation room wipe sampling, after the completion of insert
Cultivated in TSA culture mediums, if any bacteria growing, line separation single bacterium colony；Contact dish after sampling is placed in preference temperature incubator
Middle culture, it is stand-by.
2nd, settling bacteria is collected：Collect routine work and the related settling bacteria in environmental monitoring control process；
3rd, flcating germ gathers：
For TSA flat boards, sampling terminates rear TSA flat boards and culture 3d is inverted in 32 DEG C of incubators the culture medium used.
As a further improvement on the present invention, the method for the settling bacteria collection is：TSA cultures are prepared according to regulation method
TSA flat boards are prepared after base and sterilizing, flat board faces up in work top, lid is opened and stands, after the time as defined in arrival
Culture dish lid is covered, culture 3d is inverted in 32 DEG C of incubators.
After said structure, beneficial effects of the present invention are：The present invention by collecting the microorganism in food toilet,
The germ in food can be effectively controlled, food quality is improved, ensures the safe handling of food.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, it is right below in conjunction with embodiment
The present invention is further elaborated.It should be appreciated that embodiment described herein is only to explain the present invention, and
It is not used in the restriction present invention.
Embodiment：
A kind of food clean room environment microorganism-collecting method, is comprised the concrete steps that：
First, surface microorganism is collected：
(1), regular surfaces sample：Take 25cm2The position (clean bench that samples on demand respectively of disposable TSA contact dish
Surface), time of contact is set to 10s；
(2), irregular surface samples：Using sterilizing cotton swab excessive risk operation room wipe sampling, after the completion of insert
Cultivated in TSA culture mediums, if any bacteria growing, line separation single bacterium colony；Contact dish after sampling is placed in preference temperature incubator
Middle culture, it is stand-by.
2nd, settling bacteria is collected：
Collect routine work is to the related settling bacteria in environmental monitoring control process, specific method：According to regulation method
Prepare TSA culture mediums and prepare TSA flat boards after sterilizing, flat board faces up in work top, open lid and stand, reach rule
Culture dish lid is covered after the fixed time, culture 3d is inverted in 32 DEG C of incubators；
3rd, flcating germ gathers：
For TSA flat boards, sampling terminates rear TSA flat boards and culture 3d is inverted in 32 DEG C of incubators the culture medium used.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires rather than described above limits, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It is appreciated that other embodiment.
Claims (2)
- A kind of 1. food clean room environment microorganism-collecting method, it is characterised in that：Comprise the concrete steps that：First, surface microorganism is collected：(1), regular surfaces sample：Take 25cm2The position (clean bench surface) that samples on demand respectively of disposable TSA contact dish, Time of contact is set to 10s；(2), irregular surface samples：Using sterilizing cotton swab excessive risk operation room wipe sampling, after the completion of insert TSA training Support and cultivated in base, if any bacteria growing, line separation single bacterium colony；Contact dish after sampling is placed in preference temperature incubator and trained Support, it is stand-by；2nd, settling bacteria is collected：Collect routine work and the related settling bacteria in environmental monitoring control process；3rd, flcating germ gathers：For TSA flat boards, sampling terminates rear TSA flat boards and culture 3d is inverted in 32 DEG C of incubators the culture medium used.
- A kind of 2. food clean room environment microorganism-collecting method according to claim 1, it is characterised in that：The sedimentation Bacterium collect method be：TSA culture mediums are prepared according to regulation method and prepare TSA flat boards after sterilizing, in work top by flat board Face up, open lid and stand, culture dish lid is covered after the time as defined in arrival, culture is inverted in 32 DEG C of incubators 3d。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710677406.7A CN107513554A (en) | 2017-08-09 | 2017-08-09 | A kind of food clean room environment microorganism-collecting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710677406.7A CN107513554A (en) | 2017-08-09 | 2017-08-09 | A kind of food clean room environment microorganism-collecting method |
Publications (1)
| Publication Number | Publication Date |
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| CN107513554A true CN107513554A (en) | 2017-12-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710677406.7A Pending CN107513554A (en) | 2017-08-09 | 2017-08-09 | A kind of food clean room environment microorganism-collecting method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408528A (en) * | 2019-08-27 | 2019-11-05 | 杭州穆恩自动化科技有限公司 | Settling bacteria monitoring machine |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816827A (en) * | 2012-08-10 | 2012-12-12 | 杭州澳亚生物技术有限公司 | Preparation method for culture medium product for medicine industry environment microorganism monitoring |
-
2017
- 2017-08-09 CN CN201710677406.7A patent/CN107513554A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102816827A (en) * | 2012-08-10 | 2012-12-12 | 杭州澳亚生物技术有限公司 | Preparation method for culture medium product for medicine industry environment microorganism monitoring |
Non-Patent Citations (5)
| Title |
|---|
| SYLVIE M. DECKERS等: "Possible Influence of Surfactants and Proteins on the Efficiency of Contact Agar Microbiological Surface Sampling", 《JOURNAL OF FOOD PROTECTION》 * |
| 丁岚峰等: "《制药专业顶岗实习教程》", 30 October 2015, 中国轻工业出版社 * |
| 梁炜等: "医药工业洁净室（区）浮游菌的测试方法", 《医药工业洁净室（区）浮游菌的测试方法》 * |
| 王石全等: "新建疫苗车间洁净度确认及环境监测微生物数据库的建立", 《微生物学免疫学进展》 * |
| 陆维怡等: "医药工业洁净室（区）沉降菌的测试方法", 《医药工业洁净室（区）沉降菌的测试方法》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110408528A (en) * | 2019-08-27 | 2019-11-05 | 杭州穆恩自动化科技有限公司 | Settling bacteria monitoring machine |
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| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171226 |
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| RJ01 | Rejection of invention patent application after publication |