CN107513077A - Active enzyme mimics and foliar fertilizer, synergist and synergist application method based on the compound - Google Patents

Active enzyme mimics and foliar fertilizer, synergist and synergist application method based on the compound Download PDF

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CN107513077A
CN107513077A CN201710676674.7A CN201710676674A CN107513077A CN 107513077 A CN107513077 A CN 107513077A CN 201710676674 A CN201710676674 A CN 201710676674A CN 107513077 A CN107513077 A CN 107513077A
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plant
synergist
active enzyme
multifunction
enzyme mimics
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CN107513077B (en
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刘艳博
廖展如
孟祥高
李骥
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    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic System
    • C07F15/02—Iron compounds
    • C07F15/025—Iron compounds without a metal-carbon linkage
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01C—PLANTING; SOWING; FERTILISING
    • A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • A01C1/06—Coating or dressing seed
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G13/00—Protecting plants
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00—Cultivation of specific crops or plants not otherwise provided for
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N55/00—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur
    • A01N55/02—Biocides, pest repellants or attractants, or plant growth regulators, containing organic compounds containing elements other than carbon, hydrogen, halogen, oxygen, nitrogen and sulfur containing metal atoms
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
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    • C07F1/08—Copper compounds
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    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F13/00—Compounds containing elements of Groups 7 or 17 of the Periodic System
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    • C07—ORGANIC CHEMISTRY
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    • C07F15/00—Compounds containing elements of Groups 8, 9, 10 or 18 of the Periodic System
    • C07F15/06—Cobalt compounds
    • C07F15/065—Cobalt compounds without a metal-carbon linkage
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    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F3/00—Compounds containing elements of Groups 2 or 12 of the Periodic System
    • C07F3/02—Magnesium compounds
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07F—ACYCLIC, CARBOCYCLIC OR HETEROCYCLIC COMPOUNDS CONTAINING ELEMENTS OTHER THAN CARBON, HYDROGEN, HALOGEN, OXYGEN, NITROGEN, SULFUR, SELENIUM OR TELLURIUM
    • C07F3/00—Compounds containing elements of Groups 2 or 12 of the Periodic System
    • C07F3/06—Zinc compounds

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Abstract

The invention provides a kind of active enzyme mimics and the foliar fertilizer based on the compound, synergist and synergist application method, belong to biochemical field.Active enzyme mimics form activated centre by the coupling of organic ligand and metal ion, and then start the degeneration-resistant mechanism of crop itself by a variety of reaction mechanisms, it can be used in the different times of crop growth, realize crop insect pest, sterilization, anti-stain, drought resisting, cold-resistant, increasing both production and income.For the active enzyme mimics when for pest-resistant calamity, concentration reaches 500~1000ppm, and obvious side effect is not shown to plant.

Description

Active enzyme mimics and foliar fertilizer, synergist and synergy based on the compound Agent application method
Technical field
The invention belongs to agricultural technology field, more particularly to active enzyme mimics and the blade face based on the compound Fertilizer, synergist and synergist application method.
Background technology
With the continuous development in city, population and the contradiction in arable land are more and more prominent, how to improve per unit area yield to support increasingly The population of growth to the demand of grain, will turn into one it is important the problem of.The administration of chemical fertilizer is to improve effective hand of grain yield One of section, but basic chemical fertilizer (urea, ammonium nitrate, MAP, Diammonium phosphate (DAP), potassium sulfate, potassium chloride etc.) is excessively used can cause Soil hardening, pollute environment.By the builder product of biological inducing action, the degeneration-resistant mechanism of crop itself can be started, not only Increasing both production and income can be realized, while can also reduce the usage amount of chemical fertilizer, agricultural chemicals and bactericide, reduces peasant burden.
All kinds of common bio-hormones with synergistic effect, not only there is synthesis or the problem of extraction process complexity, together When can only be directed under specific crop and specific environment be applicable in most cases.Endogenous tune is relied on therefore, it is necessary to search out Control the Novel synergistic agent for mechanism of action.
The content of the invention
It is an object of the invention to provide a kind of active enzyme mimics and foliar fertilizer, synergist based on the compound And synergist application method.
The present invention is to be achieved through the following technical solutions：
A kind of active enzyme mimics for making plant stress-resistance synergy, including organic ligand, metal ion and anion；
Wherein, organic ligand is the one or more in ligand 1~12, and the structure of ligand 1~12 is as follows：
Wherein, R isIn one or more；
Wherein, metal ion Cu2+、Zn2+、Fe3+、Fee2+、Mg2+、Mo2+、Co2+、Mn2+、Mn3+And Mn4+In one kind or It is a variety of；
Wherein, anion NO3 -、Cl-、H2PO3 -、HPO3 2-、SO4 2-And ClO4 -One or more；
Wherein, the positive charge total amount of metal ion band is equal to the negative electrical charge total amount of anion band；
Wherein, according to molar amount, organic ligand：Metal ion=1：(1~3).
Preferably, described active enzyme mimics also include solvent molecule, and the solvent molecule is water, methanol, second One or more in alcohol, propyl alcohol, ethylene glycol, glycerine, pyridine, DMF, DMSO and phenol；
Wherein, according to molar amount, ratio≤3 of organic ligand and solvent molecule.
A kind of plant multifunction synergist, including described active enzyme mimics；Also include solvent, the solvent is One or more in DMF, DMSO, ethylene glycol；Active enzyme mimics are dissolved or suspended in solvent；Wherein, according to matter Amount calculates, active enzyme mimics：Solvent=1：(5~50).
A kind of application method of plant multifunction synergist, including step：Plant multifunction synergist is diluted with water to 4 ~30ppm, then vegetable seeds is soaked seed or to plant-spraying；Wherein, the plant is cereal crops or industrial crops；Spray Time include the plant germination phase, plant meet with drought when, plant meet with stain calamity when or plant meet with sick calamity when.
Preferably, if plant needs to survive the winter, before plant survives the winter or when plant turns green, plant-spraying plant multifunction is increased Imitate agent.
Preferably, if plant carries out grafting or transplanting, after grafting or transplanting, to plant-spraying plant multifunction Synergist.
Preferably, described cereal crops are rice, wheat, soybean, corn or sorghum etc.；Described industrial crops are sweet Sugarcane, eggplant, edible gourd, apple, cotton, herbage or fresh flower etc..
A kind of application method of plant multifunction synergist, including step：Plant multifunction synergist is diluted with water to 500~1000ppm, then to plant-spraying；Wherein, the plant is cereal crops or industrial crops；The opportunity sprayed is plant When thing meets with the plague of insects.
Preferably, described cereal crops are rice, wheat, soybean, corn or sorghum etc.；Described industrial crops are sweet Sugarcane, eggplant, edible gourd, apple, cotton, herbage or fresh flower etc..
A kind of compound foliar fertilizer, including foliar fertilizer, in addition to described active enzyme mimics；Wherein, organized enzyme mould The mass ratio of type compound is 0.1~0.5%.The compound foliar fertilizer, can be commercially available foliar fertilizer and organized enzyme model chemical combination The mixture of thing or on the basis of the existing commercially available foliar fertilizer to after composition and the content adjustment of wherein nutritional ingredient simultaneously Add the compound foliar fertilizer of active enzyme mimics.
Compared with prior art, the present invention has technique effect beneficial below：
The active enzyme mimics provided by the invention for making plant stress-resistance synergy, it passes through organic ligand and metal ion Coupling form activated centre, and then pass through the degeneration-resistant mechanism that a variety of reaction mechanisms start crop itself.In wheat experiment of suffering from drought Middle discovery, such active enzyme mimics can improve the photosynthetic rate of wheat, and reduce transpiration rate, improve the water of wheat Divide utilization rate, and realize the purpose of volume increase；In wheat endures cold rice shoot experiment, discovery can significantly improve wheat survival rate, Contribute to surviving the winter for plant cold resistance and overwintering plant；In the simulation experiments of various crop, pass through seed dressing and the seedling spraying work Property enzyme mimics, can be obviously improved various crop yield.Found in antibiotic property experiment, the active enzyme mimics Also there is good antibacterial action.Moreover, active enzyme mimics provided by the invention in crop important growth period and When running into the disasters such as disease, drought, stain, its dosage is 4~40ppm, and when running into the plague of insects, it can be played in 500~1000ppm good Good combats a natural disaster to act on, and when at concentrations up to 500~1000ppm, does not show obvious side effect.
Plant multifunction synergist provided by the invention, it is the concentrate of active enzyme mimics, and user is direct Being diluted with water to after suitable concentration can be easy to use to use.
The application method of plant multifunction synergist provided by the invention, used in the different times of crop growth, it is real Existing crop insect pest, sterilization, anti-stain, drought resisting, cold-resistant, increasing both production and income.
A kind of compound foliar fertilizer provided by the invention, absorption and utilization of the liquid level to fertilizer can be promoted, improve fertilizer Utilization rate.
Brief description of the drawings
Fig. 1 is the X-ray single crystal diffraction figure of active enzyme mimics 1.
Embodiment
With reference to specific embodiment, the present invention is described in further detail, it is described be explanation of the invention and It is not to limit.
Embodiment 1
The preparation of active enzyme mimics 1
1,2- cyclohexylamine tetraacethyl (cellulose triacetic acid, CTA) and 2- mercaptoanilines by amount of substance it Than 1:4, mill and enter converter after being well mixed, 170 DEG C are total to thermal condensation 8 hours untill dry-steam is released.Condensation product After being cooled to 50 DEG C, put into etc. stirring in quality absolute ethyl alcohol stand, filtering, obtain after crude product respectively with distilled water, second Alcohol and ether washing, low temperature drying obtains white powder, is part 4C, conversion ratio 67%.Part 4C structure is：.
Wherein, R is
HRMS(ESI+)m/z calculated for C38H35N6S4(M+H):703.1806,found703.1811.
Part 4C and Fe (NO3)3·9H2O is according to the ratio between amount of substance 1:1.3 mixing, are added in 95% ethanol of 2 times of quality, Stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the orange-yellow transparent stillness of night at 60 DEG C.Orange solids Powder is final products, conversion ratio 96%.It is [Fe (part 4C)] through X-ray single crystal diffraction measure product cationic structural (NO3)3。
Embodiment 2：
Diethylenetriamine pentaacetic acid (diethylenetriaminepentaacetic acid, DTPA) presses thing with o-phenylenediamine The ratio between amount of matter 1:5, mill and enter converter after being well mixed, 200 DEG C are total to thermal condensation 24 hours untill dry-steam is released. After condensation product is cooled to 50 DEG C, put into etc. stirring in quality absolute ethyl alcohol stand, filtering, obtain after crude product respectively with steaming Distilled water, ethanol and ether washing, low temperature drying obtains white powder, is part 6A, conversion ratio 72%.Part 6A structure For：
Wherein, R is
HRMS(ESI+)m/z calculated for C44H44N13(M+H):754.3843,found754.3845.
Part 6A and MnCl2·4H2O is according to the ratio between amount of substance 1:1 mixing, is added in the ethylene glycol of 2 times of quality, at 60 DEG C Lower stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the transparent stillness of night.Solid powder is final products, is turned Rate is 93%.It is [Mn (part 6A)] Cl through determination of elemental analysis product2。
Embodiment 3：
Disodium salt (EDTA) is with o-phenylenediamine by the ratio between amount of material 1:4, mill and enter converter after being well mixed, 180 DEG C are total to thermal condensation 16 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, it is anhydrous the quality such as to put into Stirring stands, filtered in ethanol, is washed respectively with distilled water, ethanol and ether after obtaining crude product, low temperature drying obtains white Powder, is ligand 1 A, conversion ratio 69%.Ligand 1 A structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C34H33N10(M+H):581.2890,found581.2891.
Ligand 1 A and CuSO4·4H2O is according to the ratio between amount of substance 1:1.1 mixing, are added in the DMF of 2 times of quality, at 60 DEG C After stirring reaction 4 hours, cooling, filtering, dry, obtain solid powder and the transparent stillness of night.Solid powder is final products, conversion Rate is 92%.Through elementary analysis, product is [Cu (ligand 1 A)] SO4。
Embodiment 4：
Double (the 2- amino-ethyls ether) tetraacethyls (EGTA) of ethylene glycol are with 2- mercaptoanilines by the ratio between amount of material 1:4, mill Enter converter after well mixed, 170 DEG C are total to thermal condensation 16 hours untill dry-steam is released.Condensation product is cooled to 50 DEG C Afterwards, put into etc. stirring in quality absolute ethyl alcohol stand, filtering, obtain after crude product respectively with glycerine, distilled water, ethanol and Ether washs, and low temperature drying obtains white powder, is part 5C, conversion ratio 66.7%.Part 5C structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C38H37N6O2S4(M+H):737.1861,found737.1865.
Part 5C and Co (NO3)2·6H2O、Ni(NO3)2·6H2O is according to the ratio between amount of substance 1:1.1:1.1 mixing, add 2 In the pyridine of times quality, stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the transparent stillness of night at 60 DEG C. Solid powder is final products, conversion ratio 81%.Through elementary analysis, product is [Co-Ni (part 5C)] (NO3)4。
Embodiment 5：
Ethylenediamine tetrapropionic acid (EDTP) is with ethylenediamine by the ratio between amount of material 1:4, mill and enter converter after being well mixed, 210 DEG C are total to thermal condensation 24 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, it is anhydrous the quality such as to put into In ethanol stirring stand, filtering, obtain after crude product that spent glycol, methanol, ethanol and ether washing, low temperature drying obtain respectively White powder, is ligand 1 0B, conversion ratio 62.5%.Ligand 1 0B structure is：
Wherein, R is
HRMS(ESI+)m/z calculated for C22H33N10(M+H):437.2890,found437.2888.
Ligand 1 0B and MnCl2·6H2O、FeCl3·6H2O is according to the ratio between amount of substance 1:1.1:1.1 mixing, add 2 times of matter In the phenol of amount, stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the transparent stillness of night at 60 DEG C.Solid Powder is final products, conversion ratio 86%.Through elementary analysis, product is [Fe-Mn (ligand 1 0B)] Cl5。
Embodiment 6：
The ratio between amount of the propionic acid of Diethylenetriamine five and o-phenylenediamine material 1:5, mill and enter converter after being well mixed, 170 DEG C Thermal condensation is total to 24 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, the quality absolute ethyl alcohol such as put into Middle stirring is stood, filtering, is washed respectively with phenol, benzene and ether after obtaining crude product, low temperature drying obtains white powder, is Ligand 1 2A, conversion ratio 70.5%.Wherein, ligand 1 2A structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C49H54N13(M+H):824.4625 found 824.4628. match somebody with somebody Body 12A and MgCl2·6H2O is according to the ratio between amount of substance 1:2.5 mixing, are added in the benzene of 2 times of quality, the stirring reaction 4 at 60 DEG C After hour, cooling, filtering, dry, obtain solid powder and the transparent stillness of night.Solid powder is final products, conversion ratio 84%. It is [Mg through elementary analysis product2(ligand 1 2A)] Cl4。
Embodiment 7：
The propionic acid of Diethylenetriamine five is with 2- mercaptoanilines by the ratio between amount of material 1:5, mill and enter converter after being well mixed, 170 DEG C are total to thermal condensation 24 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, it is anhydrous the quality such as to put into Stirring stands, filtered in ethanol, is washed respectively with glycerine, ethylene glycol and ethanol after obtaining crude product, low temperature drying obtains white Color powder, is ligand 1 2C, conversion ratio 70.5%.Ligand 1 2C structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C49H49N8S5(M+H):909.2684 found909.2683. matches somebody with somebody Body 12C and CuHPO3、Zn(ClO4)2·6H2O is according to the ratio between amount of substance 1:1.1:1.1 mixing, are added in the phenol of 2 times of quality, Stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the transparent stillness of night at 60 DEG C.Solid powder is final Product, conversion ratio 87%.Through elementary analysis, product is [Cu-Zn (ligand 1 2C)] HPO3·(ClO4)2。
Embodiment 8：
1,3- trimethylen-edinitrilo-tetraacetic acids are with 3- mercaptoanilines by the ratio between amount of material 1:4, mill and enter converter after being well mixed, 180 DEG C are total to thermal condensation 12 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, it is anhydrous the quality such as to put into Stirring stands, filtered in ethanol, is washed respectively with distilled water, ethanol and DMSO after obtaining crude product, low temperature drying obtains white Powder, is part 3C, conversion ratio 56.5%.Part 3C structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C36H33N6S4(M+H):677.1650 found677.1651. matches somebody with somebody Body 3C and Fe (ClO4)3According to the ratio between amount of substance 1:2 mixing, are added in the DMF of 2 times of quality, stirring reaction 4 hours at 60 DEG C Afterwards, cool down, filter, dry, obtain solid powder and the transparent stillness of night.Solid powder is final products, conversion ratio 79.2%.Through Elementary analysis, product are [Fe (part 3C)] (ClO4)3·3H2O。
Embodiment 9：
The ratio between amount of 1,3- propane diamine dipropyltin diacetate acid and o-phenylenediamine material 1:4, milling to enter after being well mixed turns Stove, 220 DEG C are total to thermal condensation 12 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, the quality such as put into Stirring stands, filtered in absolute ethyl alcohol, is washed respectively with distilled water, ethanol and DMSO after obtaining crude product, low temperature drying obtains White powder, is part 9A, conversion ratio 56.5%.Part 9A structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C36H37N10(M+H):609.3203 found 609.3206. match somebody with somebody Body 3A and ZnCl2、MnCl4According to the ratio between amount of substance 1:1:1 mixing, is added in the ethanol of 2 times of quality, the stirring reaction 4 at 60 DEG C After hour, cooling, filtering, dry, obtain solid powder and the transparent stillness of night.Solid powder is final products, conversion ratio 93%. Through elementary analysis, product is [Mn-Zn (part 9A)] Cl4。
Embodiment 10：
Double (the 2- amino-ethyls ether) tetraacethyls (EGTA) of ethylene glycol are with ethylenediamine by the ratio between amount of material 1:4, mixing of milling Uniformly enter converter afterwards, 170 DEG C are total to thermal condensation 16 hours untill dry-steam is released.After condensation product is cooled to 50 DEG C, Put into etc. stirring in quality absolute ethyl alcohol stand, filtering, use glycerine, distilled water, ethanol and second respectively after obtaining crude product Ether washs, and low temperature drying obtains white powder, is part 5B, conversion ratio 66.7%.Part 5B structural formula is：
Wherein, R is
HRMS(ESI+)m/z calculated for C22H33N10O2(M+H):469.2788,found469.2789.
Part 5B and Mg (H2PO3)2·6H2O is according to the ratio between amount of substance 1:2 mixing, are added in the DMSO of 2 times of quality, 60 Stirring reaction cooling, filtering, is dried after 4 hours, obtains solid powder and the transparent stillness of night at DEG C.Solid powder is final products, Conversion ratio is 74%.Through elementary analysis, product is [Mg (part 5B)] (H2PO3)2。
Embodiment 11
Wheat of suffering from drought is handled using above-described embodiment 1-10 activity enzyme mimics, and plant leaf physical signs is entered Row detection, the results are shown in Table shown in 1.
Table 1 suffer from drought wheat experiment
Embodiment 12
Endured cold rice shoot using above-described embodiment 1-10 activity enzyme mimics processing wheat, after spraying one day, to plant Physiological Index in Leaves is detected, and the results are shown in Table shown in 2.
Table 2 is endured cold rice shoot physiology, changes of biochemical indexes (wheat, do not apply relatively and intend enzyme micro- fertilizer)
Embodiment 13
Suffered from drought rice shoot using above-described embodiment 1-10 activity enzyme mimics processing cotton, after spraying one day, to plant Physiological Index in Leaves is detected, and the results are shown in Table shown in 3.
Table 3 is suffered from drought cotton test
Embodiment 14
Cotton is handled respectively using above-described embodiment 1-10 activity enzyme mimics to suffer from drought rice shoot, and it is not arid with sufficient water Cotton rice shoot carries out physiology, biochemical indicator control, as a result see the table below shown in 4.
The different disposal cotton leaf physiology of table 4, changes of biochemical indexes situation (according to arid control, ± %)
Result above shows, active enzyme mimics rice shoot of being suffered from drought to cotton can play long term, amount of activated enzyme After the arid cotton crop of model compound (embodiment 3 and embodiment 7) processing, its physiology, biochemical indicator are even better than sufficient water not Arid cotton crop.
Note：(1) can be increased by the plant proline content of drought stress.
(2) situation of relative conductivity reaction plant cell rupture, its value is higher, and reaction plant membrane permeability is higher, injured Evil degree is more serious.
(3) water stress reduces chlorophyll content of plant.
(4) SOD, CAT and POD are three kinds of important Protective enzyme in plant.As water stress increases, the SOD mono- in plant As can present first reduce after elevated trend；POD enzymatics decline chlorophyll degradation, photosynthesis rate.
Embodiment 15
In the middle part of the field in Plain, 2 mu of adjacent soils are chosen, unobstructed, no river around soil；Soil is divided into 4 Block, equal plant cotton.Cotton seed is bought from local seed stations.It is handled as follows using enzyme is intended described in embodiment 5：
The cotton seed in plot 1 is intended enzyme by 4ppm activity and soaked seed 24 hours；The cotton in plot 2 is in seedling spraying 13ppm activity Intend enzyme, the amount of spraying is 50L；The cotton seed in plot 3 is intended enzyme by 4ppm activity and soaked seed 24 hours, and cotton lives in seedling spraying 13ppm Property intend enzyme, the amount of spraying is 50L；The cotton plants and cotton seed in plot 4 do not do special processing.The cotton of two mu of grounds manages at other Aspect is identical without exception.The result of experiment is illustrated in table 5, is as a result shown, activity, which intends enzyme, can improve the yield of cotton, especially Cotton seed is soaked seed and after seedling stage sprays when simultaneously, output of cotton amplification reaches 23.4%.
The Yield Increase In Cotton result of the test of table 5
Embodiment 16
In the middle part of the field in Plain, 4 mu of adjacent soils are chosen, unobstructed, no river around soil；Soil is divided into 4 Block, plant peanut.Peanut kind is bought from local seed stations.It is handled as follows using enzyme is intended described in embodiment 4：
Normally specially treated is not done in plantation in plot one；The peanut kind in plot two is intended enzyme by 4ppm activity and soaked seed 24 hours； The peanut in plot three intends enzyme in seedling spraying 13ppm activity, and the amount of spraying is 50L；The peanut kind in plot four is intended by 4ppm activity Enzyme is soaked seed 24 hours, and peanut intends enzyme in seedling spraying 13ppm activity, and the amount of spraying is 50L.The cotton of two mu of grounds manages at other Aspect is identical without exception.The result of experiment is illustrated in table 7, is as a result shown, activity, which intends enzyme, can improve the yield of peanut, especially Cotton seed is soaked seed and after seedling stage sprays when simultaneously, output of cotton amplification reaches 14.1%.
The peanut yield increasing of table 6 is tested
Embodiment 17
In the middle part of the field in Plain, 60 mu of adjacent soils are chosen, unobstructed, no river around soil；Soil is divided into 4 Block, equal rice cultivation.Plot one is handled using enzyme is intended described in embodiment 3 with common chemical fertilizer；Plot two is increased using commercially available humic acid Imitate fertile AA and substitute common chemical fertilizer；Only specially treated is not done using common chemical fertilizer in plot three.
The seed rice in plot 1 is intended enzyme by 4ppm activity and soaked seed 24 hours, and sprays 13ppm activity in Grain Filling and intend Enzyme, the amount of spraying are 50L；Plot 2 is Humic acid synergistic fertilizer, normal to apply 25 kgs/acre；Plot 3 is Common compound fertilizer, usage amount 25 kgs/acre.The result of experiment is illustrated in table 6, is as a result shown, activity, which intends enzyme, can significantly improve the efficiency of common chemical fertilizer, Increasing production of rice amplitude is up to 20.99%.
The increasing production of rice of table 6 contrast (contrast AA, 20 mu of levels)
Embodiment 18
Embodiment 1,5, the 8 activity plan enzymes have antibacterial effect.Rice, cotton, wheat, apple and asparagus are screened respectively, Foliage-spray is carried out, contrast test is carried out with carbendazim antiseptic foliage-spray effect, as a result see the table below 8.
The antibiotic property of table 8 experiment (contrast carbendazim antiseptic, sterilizing rate %)
Note：Sterilizing rate=(initial bacterium amount number-applies bacterium amount number of surviving after 24h)/initial bacterium amount number.
To activity intend enzyme the mechanism of action, Production of Large Fields checking on the basis of, inventor by microcellular test after Continuous checking activity intends enzyme and the reverse-resisting yield-increasing of other crops is acted on, and specific Experimental results show is in embodiment 19~35.
In microcellular experiment, used test method is as follows：
A certain plot, soil environment, moisture environment, sunlight and the ventilated environment in whole plot are divided in experimental field It is basically identical.Several adjacent micro-assay cells, the area and profile base of each micro-assay cell are marked off from plot This is identical, and area is 4~6 square metres, and border is clearly demarcated between each micro-assay cell.Different micro-assay cell conducts Different treatment groups, establish clear and definite label.
Treatment group includes control group and several experimental groups, and the plant or seed in control group model by organized enzyme The processing of compound, and the plant or seed in experimental group pass through the processing that " method " one column is indicated in embodiment 19~35.Need It should be noted that in the same embodiment, each treatment group undergoes same or similar disaster-stricken process, i.e. control group calculates Yield be it is disaster-stricken after crop yield, the yield that experimental group calculates be also it is disaster-stricken after crop yield.All processing Group uses identical seed, carries out equal input and identical field management.With the computational methods of routine, processing is calculated Group relative to control group rate of growth.
In embodiment 19~35, a total of three kinds different disaster-stricken situations, i.e. drought, stain calamity and the plague of insects.
Drought is by controlling the irrigation to each micro-assay cell to realize, the mode of irrigation is sprinkling.
Stain calamity is simulated by spraying high concentration salt solution to each micro-assay cell, and this is polluted to plot And infringement, therefore only several critical active enzyme mimics are verified.
The plague of insects is simulated by the insect of active release capture, when the plague of insects is carried out, each micro-assay cell Between isolated by gauze.Wherein, the wheat plague of insects, the rice plague of insects, the cotton plague of insects, the peanut plague of insects, the tomato plague of insects, the eggplant plague of insects, The capsicum plague of insects, the cucumber plague of insects, the Kidney bean plague of insects, edible gourd plague of insects, the balsam pear plague of insects, the sponge gourd plague of insects are simulated using bollworm；Three-coloured amaranth The plague of insects, the bengal dayflower herb plague of insects, the romaine lettuce plague of insects and the radish plague of insects are simulated using cabbage caterpillar.
In embodiment 19~35, unless being illustrated, active enzyme mimics are first dissolved in 10 times of quality DMF in, be then diluted with water to experimental concentration again.Active enzyme mimics are when to crop applying, in addition to seed dressing Using spraying, the amount sprayed is 30L/ mus.
Embodiment 19
Simulation experiments are carried out under the conditions of 5ppm from 1 active enzyme mimics of embodiment, are as a result illustrated in table 9.
The synergist of table 9 is applied and volume increase result (rate of growth %)
Crop/method Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
5ppm concentration is dressed seed 2.03 3.74 3.14 2.14 0.98 3.9 2.22 0.78 - 0.72 3.77 1.89
5ppm concentration seedling stages apply 6.9 2.77 3.78 2.57 4.11 7.7 1.09 3.19 0.46 - 5.49 3.98
5ppm concentration drought is applied 4.2 3.65 5.95 1.17 2.09 4.7 2.13 3.84 - 1.98 11.3 4.24
Use above 5.75 5.21 7.17 3.99 4.17 5.56 3.39 4.98 0.42 2.22 13.21 7.01
Embodiment 20
The volume increase applied from 1 active enzyme mimics of embodiment under 10ppm seed dressings and 10ppm other conditions is tried Test, be as a result illustrated in table 10.
The synergist of table 10 is applied and volume increase result (rate of growth %)
Crop/method Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
10ppm concentration is dressed seed 2.9 5.7 4.8 3.3 3.5 3.9 4.1 4.3 1.3 2.6 17.3 9.5
10ppm concentration seedling stages apply 6.9 10.2 7.3 6.5 5.9 7.7 6.4 4.7 1.2 4 27 10.8
10ppm concentration drought is applied 4.2 9.4 8.5 4.2 6.5 4.7 7.7 6.2 2.2 3.8 21.4 8.7
Use above 7.5 11.7 10.2 7.3 7.6 7.6 7.2 6.5 2.1 4.9 33.2 11.7
Embodiment 21
Simulation experiments are carried out under the conditions of 5ppm from 3 active enzyme mimics of embodiment, are as a result illustrated in table 11.
The synergist of table 11 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
5ppm concentration is dressed seed 1.29 0.85 1.63 0.65 0.79 1.46 1.22 0.83 0.38 1.52 0.87 0.30 0.14 0.28 1.47 0.74
5ppm concentration seedling stages apply 1.69 2.23 2.06 1.11 2.28 0.91 1.25 0.85 1.36 2.54 0.36 1.05 0.15 0.51 1.81 1.31
5ppm concentration stains calamity is applied 1.78 1.67 2.70 1.73 1.76 1.53 2.50 0.49 0.88 1.97 0.89 1.61 0.05 0.83 4.75 1.78
Use above 1.95 2.70 2.53 1.75 1.67 1.51 2.08 1.16 1.21 1.61 0.98 1.44 0.12 0.64 3.83 2.03
Embodiment 22
From simulation experiments of the 3 active enzyme mimics of embodiment under 10ppm seed dressings and 40ppm other conditions, knot Fruit is illustrated in table 12.
The synergist of table 12 is applied and volume increase result (rate of growth %)
Embodiment 23
Simulation experiments are carried out under the conditions of 10ppm from 5 active enzyme mimics of embodiment, are as a result illustrated in table 13 In.
The synergist of table 13 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
10ppm concentration is dressed seed 3.32 2.17 4.19 1.67 2.03 3.74 3.14 2.14 0.98 3.9 2.22 0.78 - 0.72 3.77 1.89
10ppm concentration seedling stages apply 5.11 6.76 6.25 3.36 6.9 2.77 3.78 2.57 4.11 7.7 1.09 3.19 0.46 - 5.49 3.98
10ppm concentration drought is applied 4.23 3.98 6.43 4.11 4.2 3.65 5.95 1.17 2.09 4.7 2.13 3.84 - 1.98 11.3 4.24
Use above 6.71 9.3 8.72 6.02 5.75 5.21 7.17 3.99 4.17 5.56 3.39 4.98 0.42 2.22 13.21 7.01
Embodiment 24
From simulation experiments of the 5 active enzyme mimics of embodiment under 20ppm seed dressings and 100ppm other conditions, knot Fruit is illustrated in table 14.
The synergist of table 14 is applied and volume increase result (rate of growth %)
Crop/method Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
20ppm concentration is dressed seed 1.93 3.73 1.49 1.81 3.33 2.79 1.90 0.87 3.47 1.98 0.69 0.11 0.64 3.36 1.68
100ppm concentration seedling stages apply 5.75 5.31 2.86 5.87 2.35 3.21 2.18 3.49 6.55 0.93 2.71 0.39 1.05 4.67 3.38
100ppm concentration drought is applied 4.10 6.62 4.23 4.33 3.76 6.13 1.21 2.15 4.84 2.19 3.96 0.21 2.04 11.64 4.37
Use above 8.93 8.37 5.78 5.52 5.00 6.88 3.83 4.00 5.34 3.25 4.78 0.40 2.13 12.68 6.73
Embodiment 25
Simulation experiments are carried out under the conditions of 5ppm from 6 active enzyme mimics of embodiment, are as a result illustrated in table 15.
The synergist of table 15 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
5ppm concentration is dressed seed 3.00 1.96 3.78 1.51 1.83 3.38 2.84 1.94 0.88 3.52 2.01 0.70 0.33 0.65 3.42 1.71
5ppm concentration seedling stages apply 4.07 5.37 4.97 2.67 5.49 2.20 3.01 2.05 3.27 6.13 0.87 2.54 0.37 0.83 4.37 3.17
5ppm concentration drought is applied 4.52 4.25 6.86 4.38 4.49 3.89 6.36 1.26 2.23 5.02 2.28 4.10 0.20 2.12 12.06 4.52
Use above 8.42 11.67 10.94 7.56 7.22 6.53 9.00 5.00 5.23 6.98 4.25 6.24 0.52 2.78 16.57 8.80
Embodiment 26
From simulation experiments of the 6 active enzyme mimics of embodiment under 10ppm seed dressings and 30ppm other conditions, knot Fruit is illustrated in table 16.
The synergist of table 16 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
10ppm concentration is dressed seed 3.13 2.04 3.94 1.58 1.91 3.53 2.96 2.01 0.92 3.68 2.08 0.74 0.35 0.68 3.55 1.77
30ppm concentration seedling stages apply 3.95 5.23 4.83 2.59 5.33 2.13 2.93 1.99 3.18 5.96 0.84 2.47 0.36 1.21 4.24 3.07
30ppm concentration drought is applied 4.30 4.04 6.53 4.18 4.26 3.70 6.03 1.19 2.13 4.78 2.17 3.90 0.12 2.01 11.46 4.30
Use above 7.64 10.60 9.92 6.86 6.55 5.92 8.17 4.55 4.76 6.33 3.87 5.67 0.48 2.53 15.03 7.97
Embodiment 27
Simulation experiments are carried out under the following conditions from 6 active enzyme mimics of embodiment, are as a result illustrated in table 17.
The synergist of table 17 is applied and volume increase result (rate of growth %)
Crop/method Wheat Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth Bengal dayflower herb Romaine lettuce Radish
50ppm concentration is dressed seed 2.12 -3.4 2.07 0.52 3.66 6.89 2.65 1.21 4.85 2.78 0.96 0.45 0.89 4.69 2.36
150ppm concentration seedling stages apply 5.22 6.37 3.43 7.05 2.81 3.86 2.63 4.20 7.85 1.11 3.24 0.46 1.58 5.59 4.05
150ppm concentration drought is applied 5.14 7.80 5.00 5.09 4.42 7.23 1.42 2.54 5.69 2.57 4.65 0.14 2.40 13.73 5.14
Use above 6.88 8.93 6.18 5.90 5.33 7.34 4.09 4.27 5.68 3.46 5.08 0.42 2.26 13.52 7.17
Above case shows that concentration is unsuitable too high in seed soaking, and 10ppm seed dressings can reach effective result.
Embodiment 28
Simulation experiments are carried out under the conditions of 10ppm from 7 active enzyme mimics of embodiment, are as a result illustrated in table 18 In.
The synergist of table 18 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 7.79 5.09 9.84 3.92 4.77 8.77 7.37 5.03 2.31 9.15 5.20 1.83 1.77 1.70 8.85 4.43
10ppm concentration seedling stages apply With 11.0 6 14.6 3 13.5 2 7.27 14.9 3 6.00 8.18 5.56 8.90 16.6 6 2.36 6.91 2.74 3.38 11.8 7 8.62
The 10ppm concentration stain phases apply With 13.0 6 12.2 9 19.8 7 12.6 9 12.9 6 11.2 7 18.3 7 3.62 6.45 14.5 2 6.57 11.8 6 2.45 6.12 23.9 3 13.10
Use above 20.0 1 27.7 2 25.9 9 17.9 4 17.1 4 15.5 3 21.3 7 11.8 8 12.4 3 16.5 8 10.1 1 14.8 4 3.32 6.62 26.7 7 20.90
Embodiment 29
Simulation experiments are carried out under the conditions of 500ppm from 7 active enzyme mimics of embodiment, are as a result illustrated in table 19 In.Wherein, in the present embodiment progress synchronous with embodiment 28, it has identical reference group, 10ppm concentration seed dressings group, 10ppm Concentration seedling stage administration group.
The synergist of table 19 is applied and volume increase result (rate of growth %)
Embodiment 30
Simulation experiments are carried out under the conditions of 800ppm from 7 active enzyme mimics of embodiment, are as a result illustrated in table 20 In.Wherein, in the present embodiment and embodiment 28, embodiment 29 is synchronous carries out, there is identical reference group, 10ppm concentration to dress seed Group, 10ppm concentration seedling stage administration groups.
The synergist of table 20 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 7.79 5.09 9.84 3.92 4.77 8.77 7.37 5.03 2.31 9.15 5.20 1.83 1.77 1.70 8.85 4.43
10ppm concentration seedling stages apply With 11.0 6 14.6 3 13.5 2 7.27 14.9 3 6.00 8.18 5.56 8.90 16.6 6 2.36 6.91 2.74 3.38 11.8 7 8.62
The 800ppm concentration plagues of insects Using 12.2 8 11.5 5 18.6 8 11.9 3 12.1 8 10.5 9 17.2 7 3.40 6.06 13.6 5 6.18 11.1 5 2.30 5.75 18.7 8 12.31
Use above 18.6 1 16.9 6 23.0 3 16.6 8 15.9 4 14.4 4 19.8 7 8.05 11.5 6 15.4 2 9.40 10.8 0 4.09 6.16 21.8 4 19.44
Embodiment 31
Simulation experiments are carried out under the conditions of 10ppm from 8 active enzyme mimics of embodiment, are as a result illustrated in table 21 In.
The synergist of table 21 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 8.02 5.24 10.1 4 4.04 4.91 9.03 7.59 5.18 2.38 9.42 5.36 2.88 3.82 1.75 9.12 4.56
10ppm concentration seedling stages apply With 14.2 8 16.2 4 15.0 1 8.07 16.5 7 6.66 9.08 6.17 9.88 18.4 9 2.62 7.67 3.04 3.75 18.1 8 9.57
10ppm concentration droughts are applied With 17.0 2 11.3 1 18.2 8 11.6 7 11.9 2 10.3 7 16.9 0 3.33 5.93 13.3 6 6.04 10.9 1 3.25 5.63 22.0 2 12.05
Use above 29.0 1 19.1 1 27.2 9 18.8 4 18.0 0 16.3 1 22.4 4 12.4 7 13.0 5 17.4 1 10.6 2 11.5 8 4.49 6.95 34.1 1 14.95
Embodiment 32
From simulation experiments of the 8 active enzyme mimics of embodiment under 10ppm seed dressings and 100ppm other conditions, knot Fruit is illustrated in table 22.
The synergist of table 22 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 7.93 6.57 8.76 7.49 4.25 8.84 10.5 5 4.46 5.04 8.16 4.68 3.62 4.76 2.50 7.90 3.97
100ppm concentration seedling stages Using 10.6 5 16.1 1 18.1 4 6.99 14.3 8 5.73 7.87 5.37 8.57 16.0 1 2.27 6.61 2.94 3.22 29.0 3 8.26
100ppm concentration droughts Using 14.1 8 17.4 5 21.5 2 13.7 9 14.0 4 12.2 0 19.9 5 3.92 7.01 15.7 0 7.09 12.8 3 2.39 6.62 37.8 8 14.18
Use above 18.7 0 19.9 0 26.7 1 16.8 0 16.0 4 14.4 9 19.9 4 11.1 2 11.6 1 15.4 4 9.41 11.6 6 4.14 6.14 39.5 3 19.48
Embodiment 33
Simulation experiments are carried out under the conditions of 10ppm from 9 active enzyme mimics of embodiment, wherein, it is used first Beginning dissolution solvent is the DMSO of 10 times of quality, is as a result illustrated in table 23.
The synergist of table 23 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 8.65 5.69 10.9 2 4.35 5.29 9.79 8.17 5.57 2.54 10.1 9 5.84 2.02 1.95 1.87 9.85 4.96
10ppm concentration seedling stages apply With 10.6 0 13.9 9 12.9 3 6.96 14.3 1 5.70 7.84 5.34 8.53 15.9 4 2.25 6.58 1.93 3.21 17.3 5 8.22
10ppm concentration droughts are applied With 11.0 0 10.3 4 16.6 9 10.7 0 10.8 9 9.46 15.4 7 3.04 5.44 12.1 8 5.50 9.95 1.30 3.14 26.3 8 11.00
Use above 14.2 4 19.7 3 18.4 9 12.7 9 12.2 1 11.0 3 15.1 9 8.47 8.84 11.7 6 7.16 10.5 2 2.87 4.68 27.9 9 14.84
Embodiment 34
From simulation experiments of the 9 active enzyme mimics of embodiment under 10ppm seed dressings and 40ppm other conditions, knot Fruit is illustrated in table 24.
The synergist of table 24 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 8.57 5.60 10.8 2 4.31 5.25 9.65 8.11 5.53 2.54 10.0 7 5.72 2.01 1.95 1.87 9.74 4.87
40ppm concentration seedling stages apply With 11.3 9 15.0 7 13.9 3 7.49 15.3 8 6.18 8.43 5.73 9.17 17.1 6 2.43 7.12 2.82 2.48 12.2 3 8.88
40ppm concentration droughts are applied With 17.8 0 32.0 4 19.4 7 12.4 4 12.7 0 11.0 4 18.0 0 3.55 6.32 14.2 3 6.44 11.6 2 2.40 3.00 33.4 5 12.84
Use above 21.4 1 29.6 6 27.8 1 19.2 0 18.3 4 16.6 2 22.8 7 12.7 1 13.3 0 17.7 4 10.8 2 15.8 8 3.55 4.08 38.6 4 22.36
Embodiment 35
From simulation experiments of the 10 active enzyme mimics of embodiment under 10ppm seed dressings and 20ppm other conditions, knot Fruit is illustrated in table 25.
The synergist of table 25 is applied and volume increase result (rate of growth %)
Crop/method Wheat Rice Cotton Peanut Tomato Eggplant Capsicum Cucumber Kidney bean Edible gourd Balsam pear Sponge gourd Three-coloured amaranth The leaf of bamboo Dish Romaine lettuce Radish
10ppm concentration is dressed seed 7.71 5.04 9.75 3.88 4.72 8.68 7.29 4.98 2.29 9.06 5.15 1.82 1.75 1.68 8.77 4.39
20ppm concentration seedling stages apply With 10.8 3 14.3 2 13.2 3 7.12 14.6 1 5.87 8.00 5.44 8.71 16.3 0 2.31 6.76 2.68 3.31 11.6 2 8.44
20ppm concentration droughts are applied With 11.3 9 15.7 2 17.3 3 11.0 6 11.3 0 9.83 16.0 2 3.16 5.63 12.6 6 5.73 10.3 5 2.14 3.34 20.8 7 11.43
Use above 14.7 0 18.8 7 20.5 8 17.6 6 16.8 7 15.2 9 21.0 4 11.7 0 12.2 4 16.3 2 9.95 14.6 1 3.26 4.67 19.1 4 20.58
Embodiment 19~35 shows that active enzyme mimics provided by the invention can reach the effect of reverse-resisting yield-increasing Fruit.Especially when crop is dressed seed using active enzyme mimics, seedling spraying and it is disaster-stricken when spray be jointly processed by when, Effect of increasing production highly significant.Moreover, in order to tackle the plague of insects, from the spraying concentration of 7 active enzyme mimics of embodiment Reach 500~800ppm, can still reach the effect of volume increase, its toxic side effect is small.
It is an object of the invention to provide a kind of plant multifunction synergist, is used in the different times of crop growth, real Existing crop insect pest, sterilization, anti-stain, drought resisting, cold-resistant, increasing both production and income.Disease and insect resistance of the present invention, the principle of bactericidal action are to pass through Polyphenol oxidase reacts, and produces the derivatives such as melanin, flavones, isoflavones, while produce O2·–、H2O2With OH isoreactivities oxygen certainly The pathogen such as bacterium, virus are killed by base, and make insect digestive tract uncomfortable and food refusal；The anti-stain of the present invention, drought resisting, cold-resistant principle are By decomposing the unrighted acid in plant, the C such as hexanal, nonyl (alkene) aldehydic acid, laurylene aldehydic acid, MDA are produced3 –, C6 –, C9 –, C12 –Aldehyde, ketone, acid, ester antitoxin signaling molecule, induction crop itself produce degeneration-resistant mechanism.The present invention is applied to cotton, water On the crops such as rice, wheat, fruit tree, vegetables, trees, the effect of upgrading volume increase is realized.The invention discloses a kind of more work(of plant Can synergist, by the builder product of biological inducing action, the degeneration-resistant mechanism of crop itself can be started, have application it is convenient, Effect of increasing production is prominent, reduces the advantages of usage amount of chemical fertilizer, agricultural chemicals and bactericide, has wide popularizing application prospect.

Claims (9)

  1. A kind of 1. active enzyme mimics for making plant stress-resistance synergy, it is characterised in that including organic ligand, metal ion and Anion；
    Wherein, organic ligand is the one or more in ligand 1~12, and the structure of ligand 1~12 is as follows：
    Wherein, R isIn one or more；
    Metal ion is Cu2+、Zn2+、Fe3+、Fe2+、Mg2+、Mo2+、Mn2+、Mn3+And Mn4+In one or more；
    Anion is NO3 -、Cl-、H2PO3 -、HPO3 2-、SO4 2-And ClO4 -One or more；
    The positive charge total amount of metal ion band is equal to the negative electrical charge total amount of anion band；
    According to molar amount, organic ligand：Metal ion=1：(1~3).
  2. 2. active enzyme mimics as claimed in claim 1, it is characterised in that also including solvent molecule, the solvent point The sub one or more in water, methanol, ethanol, propyl alcohol, ethylene glycol, glycerine, pyridine, DMF, DMSO and phenol；
    Wherein, according to molar amount, ratio≤3 of organic ligand and solvent molecule.
  3. 3. a kind of plant multifunction synergist, it is characterised in that including the active catalator described in any one of claim 1~2 Compound；Also include solvent, the solvent is the one or more in DMF, DMSO, ethylene glycol；Active enzyme mimics are molten Solution suspends in a solvent；
    Wherein, according to Mass Calculation, active enzyme mimics：Solvent=1：(5~50).
  4. 4. the application method of the plant multifunction synergist described in claim 3, it is characterised in that by plant multifunction synergist 4~30ppm is diluted with water to, then vegetable seeds is soaked seed or to plant-spraying；Wherein, the plant is cereal crops or warp Ji crop；When the time sprayed includes the plant germination phase, plant meets with drought, plant is when meeting with stain calamity or plant meets with sick calamity When.
  5. 5. the application method of plant multifunction synergist as claimed in claim 4, it is characterised in that if plant needs to get over Winter, before plant survives the winter or when plant turns green, to plant-spraying plant multifunction synergist.
  6. 6. the application method of plant multifunction synergist as claimed in claim 4, it is characterised in that if plant carries out grafting Or transplanting, after grafting or transplanting, to plant-spraying plant multifunction synergist.
  7. 7. the application method of the plant multifunction synergist as described in any one of claim 4~6, it is characterised in that described Cereal crops are rice, wheat, soybean, corn or sorghum；Described industrial crops be sugarcane, eggplant, edible gourd, apple, grape, Cotton, herbage or fresh flower.
  8. 8. the application method of the plant multifunction synergist described in claim 3, it is characterised in that by plant multifunction synergist 500~1000ppm is diluted with water to, then to plant-spraying；Wherein, the plant is cereal crops or industrial crops；Spray Time for plant meet with the plague of insects when.
  9. 9. application of the active enzyme mimics in foliar fertilizer is prepared described in claim 1 or 2, it is characterised in that activity The mass ratio of enzyme mimics is 0.1~0.5%.
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CN110320354A (en) * 2019-07-24 2019-10-11 无锡市妇幼保健院 A kind of antigen retrieval buffers and application method

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CN1138587A (en) * 1995-06-16 1996-12-25 华中师范大学 Enriched imidazolyl Nitrogen coordinated SOD analog compound

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110320354A (en) * 2019-07-24 2019-10-11 无锡市妇幼保健院 A kind of antigen retrieval buffers and application method
CN110320354B (en) * 2019-07-24 2022-04-08 无锡市妇幼保健院 Antigen repairing liquid and using method thereof

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