CN107510057A - A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere - Google Patents
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere Download PDFInfo
- Publication number
- CN107510057A CN107510057A CN201710934867.8A CN201710934867A CN107510057A CN 107510057 A CN107510057 A CN 107510057A CN 201710934867 A CN201710934867 A CN 201710934867A CN 107510057 A CN107510057 A CN 107510057A
- Authority
- CN
- China
- Prior art keywords
- fat
- fish oil
- soluble tea
- tea polyphenol
- mackerel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 241000269821 Scombridae Species 0.000 title claims abstract description 66
- 235000021323 fish oil Nutrition 0.000 title claims abstract description 57
- 235000020640 mackerel Nutrition 0.000 title claims abstract description 56
- 150000008442 polyphenolic compounds Chemical class 0.000 title claims abstract description 51
- 235000013824 polyphenols Nutrition 0.000 title claims abstract description 51
- 241001122767 Theaceae Species 0.000 title claims abstract description 46
- 239000004005 microsphere Substances 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 32
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 claims abstract description 18
- -1 polyoxyethylene Polymers 0.000 claims abstract description 18
- 239000000839 emulsion Substances 0.000 claims abstract description 17
- 238000004108 freeze drying Methods 0.000 claims abstract description 17
- 238000000034 method Methods 0.000 claims abstract description 10
- FBPFZTCFMRRESA-FSIIMWSLSA-N D-Glucitol Natural products OC[C@H](O)[C@H](O)[C@@H](O)[C@H](O)CO FBPFZTCFMRRESA-FSIIMWSLSA-N 0.000 claims abstract description 9
- 229920003171 Poly (ethylene oxide) Polymers 0.000 claims abstract description 9
- 230000004888 barrier function Effects 0.000 claims abstract description 9
- 239000000600 sorbitol Substances 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 238000000605 extraction Methods 0.000 claims description 24
- 230000000694 effects Effects 0.000 claims description 9
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 claims description 8
- 229920000747 poly(lactic acid) Polymers 0.000 claims description 8
- 238000003815 supercritical carbon dioxide extraction Methods 0.000 claims description 8
- 239000000725 suspension Substances 0.000 claims description 8
- 239000003643 water by type Substances 0.000 claims description 8
- 239000008236 heating water Substances 0.000 claims description 7
- IMROMDMJAWUWLK-UHFFFAOYSA-N Ethenol Chemical compound OC=C IMROMDMJAWUWLK-UHFFFAOYSA-N 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 239000004372 Polyvinyl alcohol Substances 0.000 abstract description 7
- 229920002451 polyvinyl alcohol Polymers 0.000 abstract description 7
- 235000019198 oils Nutrition 0.000 abstract description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 6
- 230000003578 releasing effect Effects 0.000 abstract description 3
- 208000034530 PLAA-associated neurodevelopmental disease Diseases 0.000 abstract 1
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 abstract 1
- 239000008367 deionised water Substances 0.000 abstract 1
- 229910021641 deionized water Inorganic materials 0.000 abstract 1
- 229950004959 sorbitan oleate Drugs 0.000 abstract 1
- 241000251468 Actinopterygii Species 0.000 description 5
- 235000019688 fish Nutrition 0.000 description 5
- 150000002500 ions Chemical class 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- 241000736084 Scomber japonicus Species 0.000 description 3
- HHEAADYXPMHMCT-UHFFFAOYSA-N dpph Chemical compound [O-][N+](=O)C1=CC([N+](=O)[O-])=CC([N+]([O-])=O)=C1[N]N(C=1C=CC=CC=1)C1=CC=CC=C1 HHEAADYXPMHMCT-UHFFFAOYSA-N 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000442132 Lactarius lactarius Species 0.000 description 1
- 241000276618 Perciformes Species 0.000 description 1
- 241001274189 Pomatomus saltatrix Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 235000013332 fish product Nutrition 0.000 description 1
- 150000002576 ketones Chemical class 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/115—Fatty acids or derivatives thereof; Fats or oils
- A23L33/12—Fatty acids or derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention provides the preparation method of a kind of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprise the following steps：Mackerel fish head is taken to extract fish oil；By fat-soluble tea polyphenol dissolving wherein, miscella is obtained；PLA and miscella are dissolved in chloroform and acetone respectively, it is uniformly ultrasonic after blending to obtain dispersed phase；Sorbitan oleate, polyoxyethylene sorbitol acid anhydride monopalmitate and polyvinyl alcohol are added to stirring and dissolving in deionized water and obtain continuous phase；It is added to scattered in holder, dispersed phase is pressed into continuous phase by SPG microporous barriers, obtains monodispersed O/W types emulsion；It is transferred in thermostat and stirs, is finally solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；It is washed with deionized, is produced after freeze-drying.Mackerel fish oil and fat-soluble tea polyphenol complex microsphere prepared by this method carry mackerel oil mass and Tea Polyphenols amount is all higher, and inoxidizability is fine, while has good releasing effect, can be absorbed well by enteron aisle.
Description
Technical field
The present invention relates to the preparation method of a kind of mackerel fish oil and fat-soluble tea polyphenol complex microsphere.
Background technology
Mackerel, belong to Perciformes, mackerel section, chub mackerel category.Local name oil cylinder fish, blue and white fish etc., for economic fish at the middle and upper levels, mainly
Be distributed in Western North Pacific Ocean, be distributed in each marine site in China, the East Sea and the Huanghai Sea are the abundantest, be distributed in the East Sea and
The mackerel of the Huanghai Sea is divided into two kinds of common Japanese mackere and Australia chub mackerel, and the yield of East China Sea region mackerel is 20 × 10 in recent years4Ton or so, Huanghai Sea area
The yield of mackerel is in (11~12) × 104Ton, it is one of important economic fish in China.At present, the utilization of mackerel is mainly in chub mackerel
The processing of fish product, but many accessory substances such as internal organ, fish head, fish-bone, fish tail can be produced during processing, account for it
The 30~40% of body weight, these accessory substances contain the active ingredients such as abundant fat, protein, amino acid and trace element, such as
Fruit, which is not used, can cause to waste.The good method that fish oil is exactly a by-product utilized, the opposing party are extracted from mackerel fish head
Face, at present, fish oil all use microencapsulation, and microballoon is mainly used in the load of medicine, and fish oil are carried in microballoon, energy
More preferably digested and assimilated by enteron aisle, preferably protect unrighted acid to be utilized.
The content of the invention
Technical problems to be solved：It is an object of the invention to provide a kind of mackerel fish oil and fat-soluble tea polyphenol complex microsphere
Preparation method, the particle size of prepared mackerel fish oil and fat-soluble tea polyphenol complex microsphere 21.43-21.53 μm it
Between, carry mackerel oil mass and Tea Polyphenols amount is all higher, inoxidizability is fine, while has good releasing effect, can be by enteron aisle very
Good absorbs.
Technical scheme：A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil after crushing；
S2：By 0.5-0.8g fat-soluble tea polyphenols stirring and dissolving in 5-10g mackerel fish oil, miscella is obtained；
S3：4-8g PLAs and miscella are dissolved in 50-80mL chloroforms and 100-150mL acetone respectively, and are sufficiently stirred, altogether
It is uniformly ultrasonic after mixed to obtain dispersed phase；
S4：0.8-1g sorbitan oleates, 0.2-0.5g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.5-1g are gathered
Vinyl alcohol is added in 500-700mL deionized waters, and heating water bath is sufficiently stirred to 80-95 DEG C and dissolves to obtain continuous phase；
S5：It is added to scattered in holder, under impressed pressure 0.045-0.050MPa effects, will be divided by SPG microporous barriers
In dephasing press-in continuous phase, monodispersed O/W types emulsion is obtained；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, 6-8 hours are stirred with rotating speed 520-570r/min, with chloroform
With the volatilization of acetone, mackerel fish oil and fat-soluble tea polyphenol complex microsphere are finally solidified into；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged into 30-35 under rotating speed 7500-8000r/min
Minute, it is washed with deionized 3 times, is produced after freeze-drying.
Further, supercritical extract condition is that extraction temperature is 45-60 DEG C in S1, extracting pressure 35-45MPa, during extraction
Between be 2-3 hours.
It is further preferred that rotating speed is 550r/min in S6, mixing time is 7 hours.
It is further preferred that rotating speed is 7800r/min in S7, centrifugation time is 33 minutes.
Beneficial effect：The particle size of mackerel fish oil and fat-soluble tea polyphenol complex microsphere prepared by the present invention is in 21.43-
Between 21.53 μm, load mackerel oil mass is higher, reaches as high as 64.95%, and it is up to 17.44% to carry Tea Polyphenols amount, clear for DPPH
Except rate is up to 59.98%, inoxidizability is fine, while envelop rate also can reach 19.86%, has good releasing effect,
It can be absorbed well by enteron aisle.
Embodiment
Embodiment 1
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil, extraction temperature 45 after crushing
DEG C, extracting pressure 35MPa, extraction time is 2 hours；
S2：By 0.5g fat-soluble tea polyphenols stirring and dissolving in 5g mackerel fish oil, miscella is obtained；
S3：4g PLAs and miscella are dissolved in 50mL chloroforms and 100mL acetone respectively, and are sufficiently stirred, after blending uniformly
It is ultrasonic to obtain dispersed phase；
S4：0.8g sorbitan oleates, 0.2g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.5g polyvinyl alcohol are added
Enter into 500mL deionized waters, heating water bath is sufficiently stirred to 80 DEG C and dissolves to obtain continuous phase；
S5：Scattered it will be added in holder, under impressed pressure 0.045MPa effects, by SPG microporous barriers by dispersed phase pressure
Enter in continuous phase, obtain monodispersed O/W types emulsion；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, stirred 6 hours with rotating speed 520r/min, with chloroform and acetone
Volatilization, be finally solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged 30 minutes under rotating speed 7500r/min, spent
Ion water washing 3 times, is produced after freeze-drying.
Embodiment 2
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil, extraction temperature 50 after crushing
DEG C, extracting pressure 38MPa, extraction time is 2 hours；
S2：By 0.6g fat-soluble tea polyphenols stirring and dissolving in 6g mackerel fish oil, miscella is obtained；
S3：5g PLAs and miscella are dissolved in 60mL chloroforms and 110mL acetone respectively, and are sufficiently stirred, after blending uniformly
It is ultrasonic to obtain dispersed phase；
S4：0.9g sorbitan oleates, 0.3g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.6g polyvinyl alcohol are added
Enter into 550mL deionized waters, heating water bath is sufficiently stirred to 85 DEG C and dissolves to obtain continuous phase；
S5：Scattered it will be added in holder, under impressed pressure 0.046MPa effects, by SPG microporous barriers by dispersed phase pressure
Enter in continuous phase, obtain monodispersed O/W types emulsion；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, stirred 6.5 hours with rotating speed 540r/min, with chloroform and third
The volatilization of ketone, finally it is solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged 32 minutes under rotating speed 7600r/min, spent
Ion water washing 3 times, is produced after freeze-drying.
Embodiment 3
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil, extraction temperature 55 after crushing
DEG C, extracting pressure 40MPa, extraction time is 3 hours；
S2：By 0.7g fat-soluble tea polyphenols stirring and dissolving in 9g mackerel fish oil, miscella is obtained；
S3：7g PLAs and miscella are dissolved in 70mL chloroforms and 140mL acetone respectively, and are sufficiently stirred, after blending uniformly
It is ultrasonic to obtain dispersed phase；
S4：0.9g sorbitan oleates, 0.4g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.8g polyvinyl alcohol are added
Enter into 600mL deionized waters, heating water bath is sufficiently stirred to 90 DEG C and dissolves to obtain continuous phase；
S5：Scattered it will be added in holder, under impressed pressure 0.049MPa effects, by SPG microporous barriers by dispersed phase pressure
Enter in continuous phase, obtain monodispersed O/W types emulsion；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, stirred 7 hours with rotating speed 550r/min, with chloroform and acetone
Volatilization, be finally solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged 33 minutes under rotating speed 7800r/min, spent
Ion water washing 3 times, is produced after freeze-drying.
Embodiment 4
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil, extraction temperature 60 after crushing
DEG C, extracting pressure 45MPa, extraction time is 3 hours；
S2：By 0.8g fat-soluble tea polyphenols stirring and dissolving in 10g mackerel fish oil, miscella is obtained；
S3：8g PLAs and miscella are dissolved in 80mL chloroforms and 150mL acetone respectively, and are sufficiently stirred, after blending uniformly
It is ultrasonic to obtain dispersed phase；
S4：1g sorbitan oleates, 0.5g polyoxyethylene sorbitol acid anhydride monopalmitates and 1g polyvinyl alcohol are added to
In 700mL deionized waters, heating water bath is sufficiently stirred to 95 DEG C and dissolves to obtain continuous phase；
S5：Scattered it will be added in holder, under impressed pressure 0.050MPa effects, by SPG microporous barriers by dispersed phase pressure
Enter in continuous phase, obtain monodispersed O/W types emulsion；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, stirred 8 hours with rotating speed 570r/min, with chloroform and acetone
Volatilization, be finally solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged 35 minutes under rotating speed 8000r/min, spent
Ion water washing 3 times, is produced after freeze-drying.
Comparative example 1
The present embodiment compared with Example 1, does not contain polyoxyethylene sorbitol acid anhydride monopalmitate, is specifically：
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil after crushing, extraction temperature is
45-60 DEG C, extracting pressure 35-45MPa, extraction time is 2-3 hours；
S2：By 0.5-0.8g fat-soluble tea polyphenols stirring and dissolving in 5-10g mackerel fish oil, miscella is obtained；
S3：4-8g PLAs and miscella are dissolved in 50-80mL chloroforms and 100-150mL acetone respectively, and are sufficiently stirred, altogether
It is uniformly ultrasonic after mixed to obtain dispersed phase；
S4：0.8-1g sorbitan oleates and 0.5-1g polyvinyl alcohol are added in 500-700mL deionized waters, water-bath
80-95 DEG C is heated to, is sufficiently stirred and dissolves to obtain continuous phase；
S5：It is added to scattered in holder, under impressed pressure 0.045-0.050MPa effects, will be divided by SPG microporous barriers
In dephasing press-in continuous phase, monodispersed O/W types emulsion is obtained；
S6：O/W type emulsion is transferred in 15 DEG C of thermostats, 6-8 hours are stirred with rotating speed 520-570r/min, with chloroform
With the volatilization of acetone, mackerel fish oil and fat-soluble tea polyphenol complex microsphere are finally solidified into；
S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged into 30-35 under rotating speed 7500-8000r/min
Minute, it is washed with deionized 3 times, is produced after freeze-drying.
Comparative example 2
The present embodiment compared with Example 1, does not contain Tea Polyphenols, is specifically：
A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, comprises the following steps：
S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil, extraction temperature 45 after crushing
DEG C, extracting pressure 35MPa, extraction time is 2 hours；
S2：4g PLAs and mackerel oil are not dissolved in 50mL chloroforms and 100mL acetone, and are sufficiently stirred, after blending uniformly
It is ultrasonic to obtain dispersed phase；
S3：0.8g sorbitan oleates, 0.2g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.5g polyvinyl alcohol are added
Enter into 500mL deionized waters, heating water bath is sufficiently stirred to 80 DEG C and dissolves to obtain continuous phase；
S4：Scattered it will be added in holder, under impressed pressure 0.045MPa effects, by SPG microporous barriers by dispersed phase pressure
Enter in continuous phase, obtain monodispersed O/W types emulsion；
S5：O/W type emulsion is transferred in 15 DEG C of thermostats, stirred 6 hours with rotating speed 520r/min, with chloroform and acetone
Volatilization, be finally solidified into mackerel fish oil and fat-soluble tea polyphenol complex microsphere；
S6：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged 30 minutes under rotating speed 7500r/min, spent
Ion water washing 3 times, is produced after freeze-drying.
The partial properties index of mackerel fish oil and fat-soluble tea polyphenol complex microsphere prepared by the present invention see the table below 1, its particle diameter
Between 21.43-21.53 μm, load mackerel oil mass is higher, reaches as high as 64.95%, and it is up to 17.44% to carry Tea Polyphenols amount, right
59.98% is up in DPPH clearance rates, inoxidizability is fine, while envelop rate also can reach 19.86%, has and releases well
Effect is put, can be absorbed well by enteron aisle.
The partial properties index of the mackerel fish oil of table 1 and fat-soluble tea polyphenol complex microsphere
To in the measure of DPPH clearance rate, microballoon concentration is 5mg/ml.
Claims (4)
- A kind of 1. preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere, it is characterised in that：Comprise the following steps：S1：Mackerel fish head, freeze-drying is taken to use supercritical carbon dioxide extraction method extraction fish oil after crushing；S2：By 0.5-0.8g fat-soluble tea polyphenols stirring and dissolving in 5-10g mackerel fish oil, miscella is obtained；S3：4-8g PLAs and miscella are dissolved in 50-80mL chloroforms and 100-150mL acetone respectively, and are sufficiently stirred, altogether It is uniformly ultrasonic after mixed to obtain dispersed phase；S4：0.8-1g sorbitan oleates, 0.2-0.5g polyoxyethylene sorbitol acid anhydride monopalmitates and 0.5-1g are gathered Vinyl alcohol is added in 500-700mL deionized waters, and heating water bath is sufficiently stirred to 80-95 DEG C and dissolves to obtain continuous phase；S5：It is added to scattered in holder, under impressed pressure 0.045-0.050MPa effects, will be divided by SPG microporous barriers In dephasing press-in continuous phase, monodispersed O/W types emulsion is obtained；S6：O/W type emulsion is transferred in 15 DEG C of thermostats, 6-8 hours are stirred with rotating speed 520-570r/min, with chloroform With the volatilization of acetone, mackerel fish oil and fat-soluble tea polyphenol complex microsphere are finally solidified into；S7：Mackerel fish oil and fat-soluble tea polyphenol complex microsphere suspension are centrifuged into 30-35 under rotating speed 7500-8000r/min Minute, it is washed with deionized 3 times, is produced after freeze-drying.
- 2. the preparation method of a kind of mackerel fish oil according to claim 1 and fat-soluble tea polyphenol complex microsphere, its feature It is：Supercritical extract condition is that extraction temperature is 45-60 DEG C in the S1, extracting pressure 35-45MPa, and extraction time is 2-3 hours.
- 3. the preparation method of a kind of mackerel fish oil according to claim 1 and fat-soluble tea polyphenol complex microsphere, its feature It is：Rotating speed is 550r/min in the S6, and mixing time is 7 hours.
- 4. the preparation method of a kind of mackerel fish oil according to claim 1 and fat-soluble tea polyphenol complex microsphere, its feature It is：Rotating speed is 7800r/min in the S7, and centrifugation time is 33 minutes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710934867.8A CN107510057A (en) | 2017-10-09 | 2017-10-09 | A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710934867.8A CN107510057A (en) | 2017-10-09 | 2017-10-09 | A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107510057A true CN107510057A (en) | 2017-12-26 |
Family
ID=60726029
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710934867.8A Withdrawn CN107510057A (en) | 2017-10-09 | 2017-10-09 | A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107510057A (en) |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284276A (en) * | 2000-09-05 | 2001-02-21 | 四川东方绝缘材料股份有限公司 | Edible health oil containing oil soluble tea polyphenol derivative and its preparation |
| CN1608725A (en) * | 2003-10-20 | 2005-04-27 | 中国科学院大连化学物理研究所 | Prepn process of monodisperse polymer microballoon |
| US20090246321A1 (en) * | 2005-03-24 | 2009-10-01 | Clemson University | Feed Supplement Delivery System |
| CN101628019A (en) * | 2009-08-18 | 2010-01-20 | 河南工业大学 | Seabuckthorn oil-polylactide-coglycolide copolymer microspheres and preparation method thereof |
| CN101690879A (en) * | 2009-09-30 | 2010-04-07 | 浙江大学 | Method for preparing polymer micro balls with uniform grain diameter and controllable size |
| CN102151250A (en) * | 2010-02-11 | 2011-08-17 | 王汀 | Novel preparation method of solid lipid nanoparticles |
| CN102302457A (en) * | 2011-09-14 | 2012-01-04 | 中国科学院近代物理研究所 | Preparation method of ivermectin sustained-release microspheres |
| CN104689323A (en) * | 2015-01-23 | 2015-06-10 | 上海大学 | Oil-soluble drug microsphere with slow release function and preparation method thereof |
| WO2016024856A1 (en) * | 2014-08-12 | 2016-02-18 | Universidad Nacional Autónoma de México | Nanostructured systems as thermal protectors of functional ingredients in foods and food supplements |
| CN107669657A (en) * | 2016-08-02 | 2018-02-09 | 浙江医药股份有限公司新昌制药厂 | A kind of preparation method high-stability microencapsulated containing more double bond fat soluble nutrient |
-
2017
- 2017-10-09 CN CN201710934867.8A patent/CN107510057A/en not_active Withdrawn
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1284276A (en) * | 2000-09-05 | 2001-02-21 | 四川东方绝缘材料股份有限公司 | Edible health oil containing oil soluble tea polyphenol derivative and its preparation |
| CN1608725A (en) * | 2003-10-20 | 2005-04-27 | 中国科学院大连化学物理研究所 | Prepn process of monodisperse polymer microballoon |
| US20090246321A1 (en) * | 2005-03-24 | 2009-10-01 | Clemson University | Feed Supplement Delivery System |
| CN101628019A (en) * | 2009-08-18 | 2010-01-20 | 河南工业大学 | Seabuckthorn oil-polylactide-coglycolide copolymer microspheres and preparation method thereof |
| CN101690879A (en) * | 2009-09-30 | 2010-04-07 | 浙江大学 | Method for preparing polymer micro balls with uniform grain diameter and controllable size |
| CN102151250A (en) * | 2010-02-11 | 2011-08-17 | 王汀 | Novel preparation method of solid lipid nanoparticles |
| CN102302457A (en) * | 2011-09-14 | 2012-01-04 | 中国科学院近代物理研究所 | Preparation method of ivermectin sustained-release microspheres |
| WO2016024856A1 (en) * | 2014-08-12 | 2016-02-18 | Universidad Nacional Autónoma de México | Nanostructured systems as thermal protectors of functional ingredients in foods and food supplements |
| CN104689323A (en) * | 2015-01-23 | 2015-06-10 | 上海大学 | Oil-soluble drug microsphere with slow release function and preparation method thereof |
| CN107669657A (en) * | 2016-08-02 | 2018-02-09 | 浙江医药股份有限公司新昌制药厂 | A kind of preparation method high-stability microencapsulated containing more double bond fat soluble nutrient |
Non-Patent Citations (1)
| Title |
|---|
| 李药兰，等: "茶多酚-聚乳酸缓释微囊的制备研究", 《中国中药杂志》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106261239B (en) | A kind of coconut juice and preparation method thereof | |
| CN105124604B (en) | A kind of polymolecularity phytosterin ester microcapsules and preparation method and application | |
| CN106350208B (en) | A kind of preparation method of camellia oil used for cosmetic | |
| CN104705654A (en) | Preparation method for astaxanthin microcapsules | |
| CN103609751A (en) | Preparation process of water-soluble peony seed oil powder | |
| CN105476011B (en) | A kind of preparation method of soft capsule of lycopene | |
| CN101999521A (en) | Oil tea peel extract containing oil tea peel polyphenol as well as preparation method and application of oil tea peel extract | |
| CN103396879A (en) | Demulsification method in production processes for extracting tea-seed oil extraction with participation of water phase | |
| CN106929147A (en) | A kind of method for preparing oil body | |
| CN107467177A (en) | A kind of longan seed essential oil fresh applied to dragon fruit and preparation method thereof | |
| CN104388176A (en) | Method for preparing euphausia superba oil, microcapsule of euphausia superba oil and low-fluorine euphausia superba peptide by using aqueous enzymatic method | |
| CN107653055A (en) | A kind of method of ultrasonic wave added extracting tea seed oil by using enzymatic hydrolysis | |
| CN106819955A (en) | A kind of preparation technology of the stable type fish oil of decoloration deodorization | |
| CN103351945A (en) | Method for extracting camellia oleosa seed oil by weak acid aqueous solution | |
| CN106723053A (en) | A kind of water stabilization DHA microcapsules and its preparation method and application | |
| CN104614499A (en) | Method for quickly identifying illegal cooking oil | |
| JP2008266528A (en) | Method for producing fucoidan | |
| CN103610010A (en) | Fabrication method of pepper oleoresin microcapsule | |
| CN107510057A (en) | A kind of preparation method of mackerel fish oil and fat-soluble tea polyphenol complex microsphere | |
| CN103040870B (en) | Method for extracting lipide from hippocampus or syngnathus bone meal with supercritical carbon dioxide | |
| CN106801079A (en) | The method that a kind of pair of enzyme stepwise discretization Carapax Eriocheir sinensis prepare antioxidation active peptides | |
| CN106616110A (en) | Fucoxanthin plant beverage and preparation method thereof | |
| CN101390588B (en) | Grease microcapsule prepared by high-pressure homogeneous modification rice starch and method thereof | |
| CN108651667A (en) | The production method of coffee with red date beverage | |
| CN107904023A (en) | A kind of method that remaining grease is extracted in the dry shrimp slag from krill degreasing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20171226 |
|
| WW01 | Invention patent application withdrawn after publication |