CN107494229A - A kind of method taken root using yellow flag allelopathy regulation watermifoil - Google Patents
A kind of method taken root using yellow flag allelopathy regulation watermifoil Download PDFInfo
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- CN107494229A CN107494229A CN201710851943.9A CN201710851943A CN107494229A CN 107494229 A CN107494229 A CN 107494229A CN 201710851943 A CN201710851943 A CN 201710851943A CN 107494229 A CN107494229 A CN 107494229A
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- watermifoil
- yellow flag
- leaching liquor
- taken root
- rhizome
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- Agricultural Chemicals And Associated Chemicals (AREA)
- Medicines Containing Plant Substances (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention belongs to biological technical field, discloses a kind of method taken root using yellow flag allelopathy regulation watermifoil.This method includes the preparation of (1) yellow flag rhizome leaching liquor；(2) collection and processing of watermifoil explant；(3) watermifoil is cultivated.Culture counts number of taking root after terminating.Certain density yellow flag rhizome leaching liquor can remarkably promote watermifoil rhizome and take root.The present invention adjusts fox-brush algae propagation using yellow flag leaching liquor, not only accelerates the breeding of watermifoil, and based on the allelopathy between plant, being applied to sewage reparation with the configuration of other water plants for watermifoil has important directive significance by Allelopathic Effect in Plants.
Description
Technical field
The invention belongs to biological technical field, is related to a kind of technology for adjusting root system watermifoil and taking root, belongs to biotechnology
In control water body vegetation growth and the application of breeding.
Technical background
Watermifoil (Myriophyllum verticillatum), Haloragaceae (Haloragidaceae) Myriophyllum
(Myriophyllum), perennial aquatic herbaceous plant.Root-like stock is flourishing, is spread in underwater mud, section portion takes root.In lake
It is widely distributed in pool, river, reservoir or rice terrace.
Watermifoil can absorb nitrogen, phosphorus nutrition oneself in environment by root, can also utilize cauline leaf to obtain the nutrients in water
Matter, nitrogen and phosphorus absorbed by watermifoil after to synthesize the structure composition material of itself, and heavy metal and to the toxic work of watermifoil
Organic matter, it is stored in after detoxification in vivo, or be degraded.When watermifoil gathered in, migrate out aquatic ecosystem when,
Substantial amounts of nutriment also exports from water body therewith, so as to have the function that purifying water body (Cheng Wei, 2005, Jia Yifei etc.,
2015).It is relatively broad in sewage treatment project application.
More, the huge market demand is used in watermifoil engineer applied at present.The propagation method of watermifoil mainly has cuttage
And tissue culture.Tissue culture method needs special culture medium and cultural method, not easy to operate, and condition of culture is high.And cottage method is more
Simply, also have and promote watermifoil to grow by adding plant hormone class medicine, but hormone medicine cost is higher.
The content of the invention
The present invention overcomes above-mentioned weak point, there is provided it is a kind of regulate and control water plant fox-brush algae propagation method, outside watermifoil
Implant induces root growth in yellow flag rhizome leaching liquor, accelerates the breeding of watermifoil, while instruct watermifoil in sewage disposal
Middle application.
The purpose of the present invention is realized by following scheme：
One method taken root using yellow flag leaching liquor regulation watermifoil, this method are comprised the following steps：
1) yellow flag rhizome is taken, impurity elimination, is crushed after drying in the shade and obtains yellow flag rhizome powder；
2) take yellow flag rhizome powder to add water, carry out extracting at constant temperature and obtain leaching liquor；
3) supernatant is taken after step 2) leaching liquor is centrifuged, filters, obtains yellow flag rhizome leaching liquor；
4) watermifoil plant, clip explant are taken；
5) watermifoil explant is placed in yellow flag rhizome leaching liquor and cultivated.
Above-mentioned steps 1) in, preferably yellow flag rhizome powder crosses 100-200 mesh sieves.
Above-mentioned steps 2) in, the part by weight of yellow flag rhizome powder and water is 1:300~1:1000.It is preferred that yellow flag root
The part by weight of stem powder and water is 1:600~1:1000, the most preferably part by weight of yellow flag rhizome powder and water is 1:800
~1:1000.Certain density yellow flag rhizome leaching liquor can promote watermifoil rhizome to take root, and excessive concentrations leaching liquor presses down
Watermifoil processed takes root, but the too low (part by weight 1 of yellow flag rhizome powder and water of concentration:3000~1:5000), yellow flag
Rhizome leaching liquor does not have the effect of induction root growth.
Above-mentioned steps 2) in, preferably constant temperature is 24~26 DEG C of illumination, and extraction time is extraction 36~48 hours, every 10
Rock within~12 hours 10~15 minutes.
Above-mentioned steps 3) in, preferably centrifugal condition is that 5000~10000rpm is centrifuged 5~10 minutes.
Above-mentioned steps 4) in, preferably watermifoil plant is chosen and grown fine, the watermifoil plant of no disease and pests harm；Described is outer
Implant is clip watermifoil stem section, 8-10 centimetres of watermifoil explant body length at away from stem apex 8-10cm.
Above-mentioned steps 5) in, preferably the cultivation temperature of watermifoil be 24~26 DEG C, incubation time be 6~8 days, daily 12~
Illumination in 16 hours.Culture counts the watermifoil amount of taking root after terminating.
Above-mentioned steps 5) in, distilled water supplement yellow flag rhizome leaching liquor evaporation capacity is added daily, maintains leaching liquor all the time
For initial content.
Beneficial effects of the present invention compared with the prior art：The present invention is extracted by Allelopathic Effect in Plants using yellow flag
Liquid adjusts fox-brush algae propagation, can not only accelerate the breeding of watermifoil, and based on the allelopathy between plant, be watermifoil with
The configuration of other water plants, which is applied to sewage reparation, important directive significance.
Embodiment：
The present invention is further elaborated with reference to embodiments.
Embodiment 1
1) take yellow flag rhizome, cleaned up with water, dried in the shade, crushed using grinder, cross 100 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：1000 mixing, are put into triangular flask, mix
It is even.25 DEG C of illumination boxs are put into, continuous extraction 48 hours, are rocked 10 minutes within every 12 hours.
3) extraction terminates, and obtained leaching liquor pours into centrifuge tube, and 5000rpm is centrifuged 10 minutes, takes supernatant, is filtered, is obtained
To yellow flag rhizome leaching liquor.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, the clip stem section at away from stem apex 8-10cm, as explant
Body, 8-10 centimetres of watermifoil explant body length, is cleaned up, and explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
By the use of 50ml distilled water as compareing, 10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 13 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox
Tail algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：1000 mixing extract
To leaching liquor watermifoil can be promoted to take root.
Embodiment 2
1) take yellow flag rhizome, cleaned up with water, dried in the shade.Crushed using grinder, cross 200 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：800 mixing, are put into triangular flask.It is mixed
It is even.25 DEG C of illumination boxs are put into, continuous extraction 40 hours, are rocked 15 minutes within every 10 hours.
3) after 48 hours, leaching liquor pours into centrifuge tube, and 10000rpm is centrifuged 6 minutes.Supernatant is taken, is filtered.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, away from stem apex 8-10 centimeters clip stem sections, as outer
Implant.8-10 centimetres of watermifoil explant body length, is cleaned up, and explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
Control is used as by the use of 50ml distilled water.10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 13 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox
Tail algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：800 mixing extractions obtain
Leaching liquor watermifoil can be promoted to take root.
Embodiment 3
1) take yellow flag rhizome, cleaned up with water, dried in the shade, crushed using grinder, cross 100 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：600 mixing, are put into triangular flask, mix
It is even.25 DEG C of illumination boxs are put into, continuous extraction 48 hours, are rocked 10 minutes within every 12 hours.
3) extraction terminates, and obtained leaching liquor pours into centrifuge tube, and 5000rpm is centrifuged 10 minutes, takes supernatant, is filtered, is obtained
To yellow flag rhizome leaching liquor.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, the clip stem section at away from stem apex 8-10cm, as explant
Body, 8-10 centimetres of watermifoil explant body length, is cleaned up, and explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
By the use of 50ml distilled water as compareing, 10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 11 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox
Tail algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：600 mixing extractions obtain
Leaching liquor watermifoil can be promoted to take root.
Comparative example 1
1) take yellow flag rhizome, cleaned up with water, dried in the shade.Crushed using grinder, cross 100 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：200 mixing, are put into triangular flask.It is mixed
It is even.25 DEG C of illumination boxs are put into, continuous extraction 48 hours, are rocked 10 minutes within every 12 hours.
3) after 48 hours, leaching liquor pours into centrifuge tube, and 5000rpm is centrifuged 10 minutes.Supernatant is taken, is filtered.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, the clip stem section at away from stem apex 8-10cm, as explant
Body.Clean up, explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
Control is used as by the use of 50ml distilled water.10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 7 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox-brush
Algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：What 200 mixing extractions obtained
Leaching liquor suppresses watermifoil and taken root.
Comparative example 2
1) take yellow flag rhizome, cleaned up with water, dried in the shade.Crushed using grinder, cross 100 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：100 mixing, are put into triangular flask.It is mixed
It is even.25 DEG C of illumination boxs are put into, continuous extraction 48 hours, are rocked 10 minutes within every 12 hours.
3) after 48 hours, leaching liquor pours into centrifuge tube, and 5000rpm is centrifuged 10 minutes.Supernatant is taken, is filtered.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, the clip stem section at away from stem apex 8-10cm, as explant
Body.Clean up, explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
Control is used as by the use of 50ml distilled water.10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 5 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox-brush
Algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：What 100 mixing extractions obtained
Leaching liquor suppresses watermifoil and taken root.
Comparative example 3
1) take yellow flag rhizome, cleaned up with water, dried in the shade, crushed using grinder, cross 100 mesh sieves.
2) it is accurate to weigh yellow flag rhizome powder with deionized water by weight 1：3000 mixing, are put into triangular flask.It is mixed
It is even.25 DEG C of illumination boxs are put into, continuous extraction 48 hours, are rocked 10 minutes within every 12 hours.
3) after 48 hours, leaching liquor pours into centrifuge tube, and 10000rpm is centrifuged 5 minutes.Supernatant is taken, is filtered.
4) choose and grow fine, the watermifoil plant of no disease and pests harm, the clip stem section at away from stem apex 8-10cm, as explant
Body.Clean up, explant bottom blade is cut.
5) the consistent explant of thickness is chosen, is respectively put into the triangular flask that 50mL contains yellow flag rhizome leaching liquor, makes
Control is used as by the use of 50ml distilled water.10 apoplasts are put into each triangular flask.
6) it is put into illumination box, temperature is 25 DEG C, daily illumination in 16 hours.Distilled water supplement leaching liquor is added daily
Evaporation capacity, it is 50mL to maintain leaching liquor volume all the time, is cultivated 8 days.
Count the watermifoil amount of taking root：It is 9 to immerse yellow flag leaching liquor watermifoil and averagely take root several, and immerses distilled water fox-brush
Algae, which takes root for counting averagely to take root, to be counted as 9.Illustrate yellow flag rhizome powder with deionized water by weight 1：3000 mixing extractions obtain
Leaching liquor watermifoil can not be promoted to take root.
Claims (10)
- A kind of 1. method taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that this method comprises the following steps：1) yellow flag rhizome is taken, impurity elimination, is crushed after drying in the shade and obtains yellow flag rhizome powder；2) take yellow flag rhizome powder to add water, carry out extracting at constant temperature and obtain leaching liquor；3) supernatant is taken after step 2) leaching liquor is centrifuged, filters, obtains yellow flag rhizome leaching liquor；4) watermifoil plant, clip explant are taken；5) watermifoil explant is placed in yellow flag rhizome leaching liquor and cultivated.
- 2. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 1) the yellow flag rhizome powder crosses 100-200 mesh sieves.
- 3. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 2) in, the part by weight of yellow flag rhizome powder and water is 1:300~1:1000.
- 4. the method according to claim 3 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 2) in, the part by weight of yellow flag rhizome powder and water is 1:600~1:1000.
- 5. the method according to claim 3 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 2) in, the part by weight of yellow flag rhizome powder and water is 1:800~1:1000.
- 6. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 2) in, constant temperature is 24~26 DEG C of illumination, and extraction time is 36~48 hours, is rocked 10~15 minutes within every 10~12 hours.
- 7. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 3) in, centrifugal condition is that 5000r~10000pm is centrifuged 5~10 minutes.
- 8. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that step 4) the watermifoil plant described in, which is chosen, to grow fine, the watermifoil plant of no disease and pests harm；Described explant is away from stem apex Clip watermifoil stem section at 8-10cm, 8-10 centimetres of watermifoil explant body length.
- 9. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that described The cultivation temperature of watermifoil is 24~26 DEG C in step 5), and incubation time is 6~8 days, daily illumination in 12~16 hours.
- 10. the method according to claim 1 taken root using yellow flag leaching liquor regulation watermifoil, it is characterised in that described Distilled water supplement yellow flag rhizome leaching liquor evaporation capacity is added in step 5) daily, it is initial content to maintain leaching liquor all the time.
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| CN201710851943.9A CN107494229B (en) | 2017-09-19 | 2017-09-19 | Method for regulating rooting of watermifoil by using allelopathic effect of yellow flag |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110170181A (en) * | 2019-05-24 | 2019-08-27 | 运城学院 | One kind is based on the sika deer bubble withy water extract production method that comes into leaves |
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| CN1586153A (en) * | 2004-07-23 | 2005-03-02 | 中国科学院武汉植物园 | Method for quick breeding M spicatum Linn |
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN104604693A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Augustine myriophyllum tissue culture and rapid propagation method thereof |
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2017
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| CN1586153A (en) * | 2004-07-23 | 2005-03-02 | 中国科学院武汉植物园 | Method for quick breeding M spicatum Linn |
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN104604693A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Augustine myriophyllum tissue culture and rapid propagation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110170181A (en) * | 2019-05-24 | 2019-08-27 | 运城学院 | One kind is based on the sika deer bubble withy water extract production method that comes into leaves |
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