CN1586153A - Method for quick breeding M spicatum Linn - Google Patents
Method for quick breeding M spicatum Linn Download PDFInfo
- Publication number
- CN1586153A CN1586153A CNA2004100606268A CN200410060626A CN1586153A CN 1586153 A CN1586153 A CN 1586153A CN A2004100606268 A CNA2004100606268 A CN A2004100606268A CN 200410060626 A CN200410060626 A CN 200410060626A CN 1586153 A CN1586153 A CN 1586153A
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- Prior art keywords
- myriophyllum spicatum
- culture
- haloragidaceae myriophyllum
- spicatum
- haloragidaceae
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- Y02P60/216—
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses a kind of fast propagation method of Myriophyllum spicatum. Stem section of Myriophyllum spicatum is used as the cultured matter, which is sterilized, inducing cultured, differentiated, proliferated and rooting cultured to propagate Myriophyllum spicatum fast. The present invention makes it possible to propagate over 10,000 Myriophyllum spicatum seedlings with one stem section in one year, and the seedlings are used in restoring water vegetation and decorating aquarium.
Description
Technical field:
The present invention relates to the method for tissue culture of a kind of water plants, more specifically relate to a kind of method for quickly breeding of Haloragidaceae Myriophyllum spicatum (poly-grass), belong to biotechnology application in the production of pasture and water seedling in water body revegetation and aquarium.
Background technology:
Haloragidaceae Myriophyllum spicatum (poly-grass) Haloragidaceae Myriophyllum spicatum belongs to Haloragaceae fox-brush Trentepohlia, with its leaf color jade green or slightly aubergine can be for viewing and admiring, and than antipollution, being the important plant resources in the biological food chain, is again the important pioneer pasture and water in the water body revegetation.It is radial that the leaf of Haloragidaceae Myriophyllum spicatum is feather, life-like in clear water, also is the kind commonly used in the aquarium.Low at the occurring in nature reproduction coefficient, as utilize conventional cottage method, do not form large-scale production, be difficult to satisfy the demand of vegetable material in water body revegetation and the aquarium.
Tissue culture is as a kind of important means in the biological technical field, utilize the totipotency of plant cell, as: tissues such as stem apex, tender shoots, pass through cultured in vitro, produce callus, seedling differentiation, root induction, thereby form the plant of the Clonal Rapid Propagation of plant.Though tissue culture is a kind of technology of comparative maturity, its minimal medium generally adopts MS, to different plants, needs different nutrition configurations from the different vegetative stages that produce callus, seedling differentiation, root induction, and different growth conditionss.Therefore to the tissue culture of Haloragidaceae Myriophyllum spicatum, also need special medium and condition of culture.
Summary of the invention:
The objective of the invention is, a kind of Haloragidaceae Myriophyllum spicatum method of breeding fast is provided, this method is utilized tissue culture technique, with the Haloragidaceae Myriophyllum spicatum culture through sterilization, inducing culture, differentiation and propagation and culture of rootage, can breed Haloragidaceae Myriophyllum spicatum fast, to satisfy the demand in vegetable material market in water body revegetation and the aquarium.
A kind of method of quick breeding Haloragidaceae Myriophyllum spicatum, this method follow these steps to carry out:
(1) getting length is that 3～5 centimetres Haloragidaceae Myriophyllum spicatum stem section is as the Haloragidaceae Myriophyllum spicatum culture;
(2) with the Haloragidaceae Myriophyllum spicatum culture with flowing water towards Xian 30～40 minutes, clean the surface of Haloragidaceae Myriophyllum spicatum culture again with 75% cotton ball soaked in alcohol, then with 0.1% mercury chloride immersion 6～8 minutes, aseptic water washing 8～10 minutes;
(3) will insert in the inducing culture through the Haloragidaceae Myriophyllum spicatum culture that step (2) is handled, cultivated 20～25 days; Inducing culture is: add 0.09 milligram of KT (6-furans aminopurine), 0.08 milligram 2,4-D (2,4 dichlorophenoxyacetic acid), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium; Wherein every liter of MS minimal medium contains:
NH
4NO
3(ammonium nitrate) 1650 milligrams
KNO
3(potassium nitrate) 1900 milligrams
CaCl
22H
2440 milligrams of O (calcium chloride dihydrate)
MgSO
4.7H
2370 milligrams of O (magnesium sulfate)
KH
2PO
4(potassium dihydrogen phosphate) 170 milligrams
0.83 milligram of KI (potassium iodide)
H
3BO
3(boric acid) 6.2 milligrams
MnSO
44H
222.3 milligrams of O (manganese sulphate)
ZnSO
47H
28.6 milligrams of O (zinc sulphate)
Na
2MoO
42H
20.25 milligram of O (sodium molybdate)
CuSO
45H
20.025 milligram of O (copper sulphate)
CoCl
26H
20.025 milligram of O (cobalt chloride)
FeSO
47H
227.8 milligrams of O (ferrous sulfate)
37.3 milligrams of disodium ethylene diamine tetraacetates
100 milligrams of inositols
0.5 milligram in nicotinic acid
0.5 milligram of puridoxine hydrochloride
0.1 milligram of thiamine hydrochloride
2 milligrams of glycine
The rest is water;
(4) will transfer in differentiation and proliferated culture medium through the Haloragidaceae Myriophyllum spicatum culture that step (3) is cultivated, and cultivate and grew many Haloragidaceae Myriophyllum spicatum buds in 30～45 days; Differentiation with proliferated culture medium is: add 1.2 milligrams of BA (6-benzyladenine), 0.15 milligram of NAA (methyl), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium;
(5) as if the Haloragidaceae Myriophyllum spicatum bud plant division with step (4), can be used as new Haloragidaceae Myriophyllum spicatum culture, repeating step (4) can obtain a large amount of Haloragidaceae Myriophyllum spicatum buds;
(6) as if root induction in root media that the Haloragidaceae Myriophyllum spicatum bud of step (4) is transferred, cultivate and promptly obtained the Haloragidaceae Myriophyllum spicatum seedling in 20～30 days; Root media is: add 1 liter of distilled water, 4 milligrams of BA (6-benzyladenine), 4 milligrams of IAA (methyl), 40 gram sucrose, 12 gram agar in every liter of MS minimal medium.
The present invention has following advantage: inducing culture of the present invention, differentiation are best suited for three different vegetative stages of Haloragidaceae Myriophyllum spicatum--the optimal medium prescription of-inducing culture, differentiation and propagation and culture of rootage with proliferated culture medium and root media, a Haloragidaceae Myriophyllum spicatum stem Duan Jing breeds repeatedly, can breed strain seedlings up to ten thousand in 1 year, for water body revegetation and aquarium setting provide a large amount of seedlings.
Embodiment:
A kind of method of quick breeding Haloragidaceae Myriophyllum spicatum, this method follow these steps to carry out:
(1) getting length is that 3～5 centimetres Haloragidaceae Myriophyllum spicatum stem section is as the Haloragidaceae Myriophyllum spicatum culture;
(2) with the Haloragidaceae Myriophyllum spicatum culture with flowing water towards Xian 30～40 minutes, clean the surface of Haloragidaceae Myriophyllum spicatum culture again with 75% cotton ball soaked in alcohol, then with 0.1% mercury chloride immersion 6～8 minutes, aseptic water washing 8～10 minutes;
(3) will insert in the inducing culture through the Haloragidaceae Myriophyllum spicatum culture that step (2) is handled, cultivated 20～25 days, the Haloragidaceae Myriophyllum spicatum culture begins to sprout, grow; Inducing culture is: add 0.09 milligram of KT (6-furans aminopurine), 0.08 milligram 2,4-D (2,4 dichlorophenoxyacetic acid), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium; Wherein every liter of MS minimal medium contains:
NH
4NO
3(ammonium nitrate) 1650 milligrams
KNO
3(potassium nitrate) 1900 milligrams
CaCl
22H
2440 milligrams of O (calcium chloride dihydrate)
MgSO
4.7H
2370 milligrams of O (magnesium sulfate)
KH
2PO
4(potassium dihydrogen phosphate) 170 milligrams
0.83 milligram of KI (potassium iodide)
H
3BO
3(boric acid) 6.2 milligrams
MnSO
44H
222.3 milligrams of O (manganese sulphate)
ZnSO
47H
28.6 milligrams of O (zinc sulphate)
Na
2MoO
42H
20.25 milligram of O (sodium molybdate)
CuSO
45H
20.025 milligram of O (copper sulphate)
CoCl
26H
20.025 milligram of O (cobalt chloride)
FeSO
47H
227.8 milligrams of O (ferrous sulfate)
37.3 milligrams of disodium ethylene diamine tetraacetates
100 milligrams of inositols
0.5 milligram in nicotinic acid
0.5 milligram of puridoxine hydrochloride
0.1 milligram of thiamine hydrochloride
2 milligrams of glycine
The rest is water;
(4) will transfer in differentiation and proliferated culture medium through the Haloragidaceae Myriophyllum spicatum culture that step (3) is cultivated, and cultivate and grew many Haloragidaceae Myriophyllum spicatum buds in 30～45 days; Differentiation with proliferated culture medium is: add 1.2 milligrams of BA (6-benzyladenine), 0.15 milligram of NAA (methyl), 30 gram sucrose, 6 gram agar in every liter of MS minimal medium;
(5) as if the Haloragidaceae Myriophyllum spicatum bud plant division with step (4), can be used as new Haloragidaceae Myriophyllum spicatum culture, repeating step (4) can obtain a large amount of Haloragidaceae Myriophyllum spicatum buds;
(6) as if root induction in root media that the Haloragidaceae Myriophyllum spicatum bud of step (4) is transferred, cultivate and promptly obtained the Haloragidaceae Myriophyllum spicatum seedling in 20～30 days; Root media is: add 1 liter of distilled water, 4 milligrams of BA (6-benzyladenine), 4 milligrams of IAA (methyl), 40 gram sucrose, 12 gram agar in every liter of MS minimal medium.
Implement the present invention, can obtain a large amount of Haloragidaceae Myriophyllum spicatum test-tube plantlets in a short time.
Claims (1)
1, a kind of method of quick breeding Haloragidaceae Myriophyllum spicatum is characterized in that, this method follows these steps to carry out:
(1) getting length is that 3～5 centimetres Haloragidaceae Myriophyllum spicatum stem section is as the Haloragidaceae Myriophyllum spicatum culture;
(2) with the Haloragidaceae Myriophyllum spicatum culture with flowing water towards Xian 30～40 minutes, clean the surface of Haloragidaceae Myriophyllum spicatum culture again with 75% cotton ball soaked in alcohol, then with 0.1% mercury chloride immersion 6～8 minutes, aseptic water washing 8～10 minutes;
(3) will insert in the inducing culture through the Haloragidaceae Myriophyllum spicatum culture that step (2) is handled, cultivated 20～25 days; Inducing culture is: 0.09 milligram of 6-furans aminopurine of adding, 0.08 milligram of 2,4 dichlorophenoxyacetic acid, 30 gram sucrose, 6 restrain agar in every liter of MS minimal medium;
(4) will transfer in differentiation and proliferated culture medium through the Haloragidaceae Myriophyllum spicatum culture that step (3) is cultivated, and cultivate and grew many Haloragidaceae Myriophyllum spicatum buds in 30～45 days; Differentiation with proliferated culture medium is: 1.2 milligrams of 6-benzyladenines of adding, 0.15 milligram of methyl, 30 gram sucrose, 6 restrain agar in every liter of MS minimal medium;
(5) with the Haloragidaceae Myriophyllum spicatum bud plant division of step (4), as new Haloragidaceae Myriophyllum spicatum culture, repeating step (4) can obtain a large amount of Haloragidaceae Myriophyllum spicatum buds;
(6) the Haloragidaceae Myriophyllum spicatum bud of step (4) is transferred root induction in root media is cultivated and was promptly obtained the Haloragidaceae Myriophyllum spicatum seedling in 20～30 days; Root media is: 1 liter of distilled water of adding, 4 milligrams of 6-benzyladenines, 4 milligrams of methyls, 40 gram sucrose, 12 restrain agar in every liter of MS minimal medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100606268A CN1303875C (en) | 2004-07-23 | 2004-07-23 | Method for quick breeding M spicatum Linn |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100606268A CN1303875C (en) | 2004-07-23 | 2004-07-23 | Method for quick breeding M spicatum Linn |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1586153A true CN1586153A (en) | 2005-03-02 |
| CN1303875C CN1303875C (en) | 2007-03-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100606268A Expired - Fee Related CN1303875C (en) | 2004-07-23 | 2004-07-23 | Method for quick breeding M spicatum Linn |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN103053311A (en) * | 2013-01-09 | 2013-04-24 | 成都华都农业发展有限责任公司 | Method for reproducing watermifoil |
| CN103609423A (en) * | 2013-11-19 | 2014-03-05 | 湖北工业大学 | Method for cage cultivation of myriophyllum spicatum |
| CN104604692A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Myriophyllum aquaticum tissue culture and rapid propagation method thereof |
| CN104604693A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Augustine myriophyllum tissue culture and rapid propagation method thereof |
| CN107494229A (en) * | 2017-09-19 | 2017-12-22 | 江苏省中国科学院植物研究所 | A kind of method taken root using yellow flag allelopathy regulation watermifoil |
| CN110150043A (en) * | 2019-04-28 | 2019-08-23 | 南京中科水治理股份有限公司 | A kind of cultivation of Haloragidaceae Myriophyllum spicatum terrestrial seedling, method for transplanting |
| CN110839531A (en) * | 2019-12-06 | 2020-02-28 | 水生藻安生物科技（武汉）有限公司 | Tissue culture method of ornamental water plant Antarctic fir |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1162333C (en) * | 2001-12-17 | 2004-08-18 | 中国科学院武汉植物研究所 | Method of restoring nutrition enriched shallow lake aquatic vegetation |
-
2004
- 2004-07-23 CN CNB2004100606268A patent/CN1303875C/en not_active Expired - Fee Related
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102450215A (en) * | 2010-11-01 | 2012-05-16 | 南京中科水治理工程有限公司 | Technology for propagating watermifoil through hormone induction |
| CN102450215B (en) * | 2010-11-01 | 2013-04-17 | 南京中科水治理股份有限公司 | Technology for propagating watermifoil through hormone induction |
| CN103053311A (en) * | 2013-01-09 | 2013-04-24 | 成都华都农业发展有限责任公司 | Method for reproducing watermifoil |
| CN103609423A (en) * | 2013-11-19 | 2014-03-05 | 湖北工业大学 | Method for cage cultivation of myriophyllum spicatum |
| CN104604692A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Myriophyllum aquaticum tissue culture and rapid propagation method thereof |
| CN104604693A (en) * | 2015-01-30 | 2015-05-13 | 中国科学院水生生物研究所 | Augustine myriophyllum tissue culture and rapid propagation method thereof |
| CN104604692B (en) * | 2015-01-30 | 2016-08-17 | 中国科学院水生生物研究所 | A kind of powder green watermifoil tissue culture and rapid proliferation method |
| CN104604693B (en) * | 2015-01-30 | 2016-09-07 | 中国科学院水生生物研究所 | A kind of ancient watermifoil tissue culture and rapid proliferation method difficult to understand |
| CN107494229A (en) * | 2017-09-19 | 2017-12-22 | 江苏省中国科学院植物研究所 | A kind of method taken root using yellow flag allelopathy regulation watermifoil |
| CN110150043A (en) * | 2019-04-28 | 2019-08-23 | 南京中科水治理股份有限公司 | A kind of cultivation of Haloragidaceae Myriophyllum spicatum terrestrial seedling, method for transplanting |
| CN110150043B (en) * | 2019-04-28 | 2021-05-25 | 南京中科水治理股份有限公司 | Method for cultivating and transplanting hirsutella suiffii land seedlings |
| CN110839531A (en) * | 2019-12-06 | 2020-02-28 | 水生藻安生物科技（武汉）有限公司 | Tissue culture method of ornamental water plant Antarctic fir |
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| Publication number | Publication date |
|---|---|
| CN1303875C (en) | 2007-03-14 |
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