CN107493938A - Chinese cabbage cold-resistant method for culturing seedlings under a kind of low temperature stress - Google Patents
Chinese cabbage cold-resistant method for culturing seedlings under a kind of low temperature stress Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/60—Flowers; Ornamental plants
- A01G22/67—Dwarf trees, e.g. bonsai
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Abstract
The invention discloses Chinese cabbage cold-resistant method for culturing seedlings under a kind of low temperature stress, belong to botanical seedling culturing technical field.The present invention carries out cold-resistant management using Abscisic Acid combination blade face supplementary fertilizer mode according to extremely frigid zones Temperature Features to Chinese cabbage, after seedling development to two leaves wholeheartedly afterwards to foliage-spray Abscisic Acid, 1 time a day, simultaneously daily to blade face supplementary fertilizer 1 time, and a water was sprayed in 2~3 days, bed soil water content is kept 70~75%；Method for culturing seedlings of the present invention comprises the following steps：Initial point selection, select seedlings nursing plate, seedlings nursing plate sterilization, seed disinfection, constant temperature vernalization, allotment seedling medium, filling substrate, seeding and growing seedling, cold-resistant management and go out garden；Nursery of the present invention improves resistance of the Chinese cabbage seedling to cold snap, effectively prevent the growth inhibition of seedling rhizome and blade, and plays certain prevention and control of plant diseases, pest control effect.
Description
Technical field
The present invention relates to Chinese cabbage cold-resistant method for culturing seedlings under botanical seedling culturing technical field, more particularly to a kind of low temperature stress.
Background technology
Chinese cabbage, biennial herb, high 40~60 centimetres, normal complete stool is hairless, there is a small number of setas in adaxial surface of the leaf middle arteries sometimes, category
Cruciferae Brassica genus uses vegetables.South is introduced a fine variety after originating in north of China, it is with tender leaf-head, lotus throne leaf or scape for eating
With, can stir-fry and eat, make soup, pickling, with pakchoi together turn into China's resident's dining table on it is essential together with U.S. vegetable.In China north
In the winter of side, Chinese cabbage is even more the regular guest on dining table, therefore has saying for " winter Chinese cabbage being beautiful such as bamboo shoot ".Chinese cabbage has higher nutrition
Value, therefore has the saying of " hundred dishes be not so good as Chinese cabbage ", in Chinese cabbage containing enrich crude fibre, B family vitamin, vitamin C, calcium, iron,
Phosphorus, zinc etc., the blood coagulation hemostatic enzyme in Chinese cabbage can be pre- anti-stroke, and molybdenum can also give protection against cancer；Containing 90% string inside Chinese cabbage, this
A little strings can promote the wriggling of stomach and intestine, promote the health of digestive system；The traditional Chinese medical science thinks that its cold nature of Chinese cabbage is nontoxic, through often feeding
With with nourishing the stomach to improve the production of body fluid, relieving restlessness quench one's thirst, diuresis defaecation, it is clearing heat and detoxicating the effect of.
Chinese cabbage cultivation is typically sowed in August, but because its demand is big, the application of booth vegetable technology in addition, Chinese cabbage one
One of an important factor for four seasons in year are all planting, and Cabbage Seedling technology is then influence Chinese cabbage yield and quality.Although people are white
Study and summed up compared with mature technology system in terms of the nursery of dish, but due to by season, temperature, moisture, percentage of seedgermination etc.
The influence of factor, seedling matter are also uneven.Especially in highlands, winter temperature is low, and day and night temperature is big, the cold-resistant of Chinese cabbage seedling
Property is poor, causes seedling small and weak, growth inhibition, finally influences subsequent plant strain growth and yield and quality.
Plant hormone ABA (abscisic acid) is the startup factor of Cold resistant genes expression, and the regulation and control to plant cold resistance power rise emphatically
Act on, also referred to as " adverse circumstance hormone ".The equilibrium condition in plant can be changed using Exogenous ABA to induce the table of Cold resistant genes
Reach, so as to strengthen the cold hardiness of plant.Important regulation is also played in the environment stress reaction such as plant arid, low temperature and pest and disease damage to make
With.Research finds that Exogenous ABA can improve the winter resistance of the plants such as rice, wheat, iris, cinnamomum camphora tree.
Chinese patent CN201610061002.0 discloses a kind of method for culturing seedlings of extremely frigid zones Chinese cabbage in the prior art, bag
Include Seeds preprocess, configuration bed soil, sowing, seedbed management, the prevention and control of plant diseases, pest control after emergence, the invention is by keeping the temperature of nursery stage
Degree, the mode of extra-nutrition material prevent seedling rhizome and the blade by low temperature freezing-disaster.But keep nursery temperature in extremely frigid zones
It is high to spend cost, nursery is once transplanted out chamber planting, is influenceed that cold hardiness is weak, and the plant death rate is high by the extraneous outdoor temperature difference.
The content of the invention
In order to solve above-mentioned technical problem present in prior art, the present inventor provides Chinese cabbage under a kind of low temperature stress and resisted
Cold method for culturing seedlings, to cultivate with cold-resistant, compared with Johnson ＆ Johnson's growing way, can preventing disease and pest Chinese cabbage seedling, comprise the following steps：
1) initial point selection：From quality better, the kind that yield is high, disease-resistant；
2) seedlings nursing plate is selected：According to seed type selecting or make seedlings nursing plate；
3) seedlings nursing plate sterilizes：Seedlings nursing plate is cleaned or sterilized；
4) seed disinfection：Seed is carried out disinfection using instantaneous high-temperature；
5) constant temperature vernalization：Seed is put into insulating box vernalization, until seed germination；
6) seedling medium is allocated：Mud, tealeaf residue, rotten mosses ash and organic fertilizer are mixed into seedling medium；
7) filling substrate：Matrix is filled into the hole of seedlings nursing plate；
8) seeding and growing seedling：Punched in matrix, seed is uniformly sown into the center of cavities, then entirety is uniform on seedlings nursing plate
One layer of matrix is covered, and is suitably watered according to different ambient humidities；
9) cold-resistant management：Seed plate after planting is placed in greenhouse, carries out daily management；Seedling development to two leaves wholeheartedly after
To foliage-spray plant hormone ABA (abscisic acid), 1 time a day, while daily to blade face supplementary fertilizer 1 time, and one was sprayed in every 2~3 days
Secondary water, bed soil water content is kept 70~75%；
10) garden is gone out：After seedling reaches strong sprout standard, you can transplanted.
It is to the specific sterilization method of seedlings nursing plate in the step 3)：The residual matrix in seed plate is first removed, is rushed with clear water
Wash clean, dry, and soaked 12 hours with 500 times of liquid of carbendazim or soaked 30 minutes with 1000 times of liquid of potassium permanganate and sterilized.
When being carried out disinfection in the step 4) to seed, the temperature of sterilization is preferably 90 DEG C；It is molten to be then placed in potassium permanganate
Soaked 25 to 35 minutes in liquid.
Further, the concentration of the liquor potassic permanganate in step 4) is 0.1~0.2%, and soak time is 30 minutes, finally
Sterile water wash seed is used again 2~3 times, obtain sterilized seed.
The time that seed puts insulating box into the step 5) is 2 to 3 days.
Mud, rotten mosses ash, the ratio of tealeaf residue are 6 in the step 6)：3:0.5~1.5.
In the step 9), the plastic house has plastic sheeting, sunshade net and fly net.
Further, in step 9), plant hormone ABA spraying times are 10 points of morning, are sprayed with the ABA solution of 10mg/L concentration
Blade face is applied, it is 1L/4m to squirt dosage2。
Further, in step 9), blade face supplementary fertilizer fertilizer is 0.2% potassium dihydrogen phosphate or 1% red (white) sugar.
Further, in step 9), the control of seedling rearing room day temperature is 5~15 DEG C, and night temperatures control is -1~5 DEG C.
In the step 10), seedlings nursing plate was emerged through 2~3 days, and seedling can be transplanted after 30~35 day growth phases.
The beneficial effects of the invention are as follows：
(1) method for culturing seedlings of the invention is directed to extremely frigid zones Temperature Features, and natural temperature is simulated during nursery using low
Incubate seedling, to foliage-spray plant hormone ABA (abscisic acid) after Chinese cabbage seedling goes out leaf, effectively prevent seedling rhizome and blade by
Influenceed to low temperature freezing-disaster, and certain prevention and control of plant diseases, pest control effect is played to Chinese cabbage seedling；
(2) 0.2% appropriate potassium dihydrogen phosphate is sprayed to Chinese cabbage seedling or 1% red (white) sugar gives blade face supplementary fertilizer, also improved
Seedling is allowed to be not inhibited in the growth of extremely frigid zones to the resistance of cold snap.
(3) nursery is using mud, rotten mosses ash and tealeaf residue as matrix, and nutrient formulation is reasonable, the robust seedling of cultivation, root system hair
Reach, growth is fast, and bud differentiation is good；Tealeaf residue contains the nutrients such as vitamin, inorganic salts, carbohydrate, can be protected for making base-material
Soil property moisture is held, as vegetable fertilizer, helps the development and breeding of vegetables.
Embodiment
Limited with reference to specific embodiment technical scheme is further, but claimed
Scope is not only limited to made description.
Embodiment one
Chinese cabbage cold-resistant nursery step is as follows under low temperature stress：
1. initial point selection
From quality better, the Chinese cabbage seed that yield is high, disease-resistant.
2. seedlings nursing plate selects
Selection seedlings nursing plate is 100 holes, and 54 × 28cm floppy disks, per cave back cut diameter 3.8cm, go to the bottom 2.0cm, and cave is deep
4.6cm seedlings nursing plate.
3. seedlings nursing plate sterilizes
Used hole tray may infect remain some pathogens, worm's ovum, so need to clean seedlings nursing plate and
Disinfect.Method is first to remove the residual matrix in seed plate, is rinsed well, dried with clear water, and is soaked with 500 times of liquid of carbendazim
Steep 12 hours or soaked 30 minutes with 1000 times of liquid of potassium permanganate and sterilized, also can use formalin, Eusol to carry out disinfection；
Sterilized hole tray thoroughly must dried before with clear water is clean.
4. seed disinfection
Because seed can carry certain germ in maternal plant, it is therefore desirable to which carry out disinfection processing to seed.Specifically disappear
Malicious method is as follows：First, seed is carried out disinfection using instantaneous high-temperature, the temperature of sterilization is preferably 90 DEG C, is then placed in concentration
To be soaked in 0.1% liquor potassic permanganate 25 minutes, sterile water wash seed is finally used again 2 times.
5th, constant temperature vernalization
Put seed into insulating box vernalization, after constant temperature is handled, the percentage of seedgermination in insulating box is more consistent, seed
The time sprouted for 2~3 days.
6th, seedling medium is allocated
Seedling medium is mixed into using mud, tealeaf residue and rotten mosses ash；Mud, rotten mosses ash, the ratio of tealeaf residue are 6：3:
0.5；Described mud, tealeaf residue and rotten mosses ash, without any germ, there is good water suction to inhale fertile, moisturizing and mend through high-temperature sterilization
The effect of fertilizer.
7th, filling substrate
Matrix is fully soaked, is advisable with humidity 60%, or with holding one matrix, is extruded without moisture, hold it is agglomerating,
Touch and then dissipate, matrix is uniformly filled into the hole of seedlings nursing plate.
8th, seeding and growing seedling
Punched in matrix, seed is uniformly sown into the center of cavities, then overall one layer of base of uniform fold on seedlings nursing plate
Matter, and suitably watered according to different ambient humidities.
9th, cold-resistant management
Seed plate after planting is placed in greenhouse, plastic house has plastic sheeting, sunshade net and fly net, carries out daily pipe
Reason；Seedling development is to two leaves wholeheartedly afterwards to foliage-spray plant hormone ABA (abscisic acid), and spraying time is 10 points of morning, concentration
For 10mg/L, it is 1L/4m to squirt dosage2, sprinkling 1 time daily；Simultaneously daily to blade face supplementary fertilizer 1 time, blade face supplementary fertilizer fertilizer is 1%
White sugar, and a water was sprayed in every 2 days, bed soil water content is kept 70%；It is 5 DEG C to control the control of seedling rearing room day temperature, night
Temperature control is -1 DEG C.
10th, garden is gone out
After seedling reaches strong sprout standard, you can transplanted.Seedlings nursing plate was emerged through 2~3 days, after 30~35 day growth phases
Seedling can be transplanted.
Embodiment two
Chinese cabbage cold-resistant nursery step is as follows under low temperature stress：
1. initial point selection
From quality better, the Chinese cabbage seed that yield is high, disease-resistant.
2. seedlings nursing plate selects
Selection seedlings nursing plate is 100 holes, and 54 × 28cm floppy disks, per cave back cut diameter 3.8cm, go to the bottom 2.0cm, and cave is deep
4.6cm seedlings nursing plate.
3. seedlings nursing plate sterilizes
Used hole tray may infect remain some pathogens, worm's ovum, so need to clean seedlings nursing plate and
Disinfect.Method is first to remove the residual matrix in seed plate, is rinsed well, dried with clear water, and is soaked with 500 times of liquid of carbendazim
Steep 12 hours or soaked 30 minutes with 1000 times of liquid of potassium permanganate and sterilized, also can use formalin, Eusol to carry out disinfection；
Sterilized hole tray thoroughly must dried before with clear water is clean.
4. seed disinfection
Because seed can carry certain germ in maternal plant, it is therefore desirable to which carry out disinfection processing to seed.Specifically disappear
Malicious method is as follows：First, seed is carried out disinfection using instantaneous high-temperature, the temperature of sterilization is preferably 90 DEG C, is then placed in concentration
To be soaked in 0.2% liquor potassic permanganate 35 minutes, sterile water wash seed is finally used again 3 times.
5th, constant temperature vernalization
Put seed into insulating box vernalization, after constant temperature is handled, the percentage of seedgermination in insulating box is more consistent, seed
The time sprouted for 2~3 days.
6th, seedling medium is allocated
Seedling medium is mixed into using mud, tealeaf residue and rotten mosses ash；Mud, rotten mosses ash, the ratio of tealeaf residue are 6：3:
1.5；Described mud, tealeaf residue and rotten mosses ash, without any germ, there is good water suction to inhale fertile, moisturizing and mend through high-temperature sterilization
The effect of fertilizer.
7th, filling substrate
Matrix is fully soaked, is advisable with humidity 60%, or with holding one matrix, is extruded without moisture, hold it is agglomerating,
Touch and then dissipate, matrix is uniformly filled into the hole of seedlings nursing plate.
8th, seeding and growing seedling
Punched in matrix, seed is uniformly sown into the center of cavities, then overall one layer of base of uniform fold on seedlings nursing plate
Matter, and suitably watered according to different ambient humidities.
9th, cold-resistant management
Seed plate after planting is placed in greenhouse, plastic house has plastic sheeting, sunshade net and fly net, carries out daily pipe
Reason；Seedling development is to two leaves wholeheartedly afterwards to foliage-spray plant hormone ABA (abscisic acid), and spraying time is 10 points of morning, concentration
For 10mg/L, it is 1L/4m to squirt dosage2, sprinkling 1 time daily；Simultaneously daily to blade face supplementary fertilizer 1 time, blade face supplementary fertilizer fertilizer is 1%
Brown sugar, and a water was sprayed in every 2~3 days, bed soil water content is kept 75%；It is 15 DEG C to control the control of seedling rearing room day temperature,
Night temperatures control is 5 DEG C.
10th, garden is gone out
After seedling reaches strong sprout standard, you can transplanted.Seedlings nursing plate was emerged through 2~3 days, after 30~35 day growth phases
Seedling can be transplanted.
Embodiment three
Chinese cabbage cold-resistant nursery step is as follows under low temperature stress：
1st, initial point selection
From quality better, the Chinese cabbage seed that yield is high, disease-resistant.
2nd, seedlings nursing plate selects
Selection seedlings nursing plate is 100 holes, and 54 × 28cm floppy disks, per cave back cut diameter 3.8cm, go to the bottom 2.0cm, and cave is deep
4.6cm seedlings nursing plate.
3rd, seedlings nursing plate sterilizes
Used hole tray may infect remain some pathogens, worm's ovum, so need to clean seedlings nursing plate and
Disinfect.Method is first to remove the residual matrix in seed plate, is rinsed well, dried with clear water, and is soaked with 500 times of liquid of carbendazim
Steep 12 hours or soaked 30 minutes with 1000 times of liquid of potassium permanganate and sterilized, also can use formalin, Eusol to carry out disinfection；
Sterilized hole tray thoroughly must dried before with clear water is clean.
4th, seed disinfection
Because seed can carry certain germ in maternal plant, it is therefore desirable to which carry out disinfection processing to seed.Specifically disappear
Malicious method is as follows：First, seed is carried out disinfection using instantaneous high-temperature, the temperature of sterilization is preferably 90 DEG C, is then placed in concentration
To be soaked in 0.15% liquor potassic permanganate 30 minutes, sterile water wash seed is finally used again 3 times.
5th, constant temperature vernalization
Put seed into insulating box vernalization, after constant temperature is handled, the percentage of seedgermination in insulating box is more consistent, seed
The time sprouted for 2~3 days.
6th, seedling medium is allocated
Seedling medium is mixed into using mud, tealeaf residue and rotten mosses ash；Mud, rotten mosses ash, the ratio of tealeaf residue are 6：3:
1；Described mud, tealeaf residue and rotten mosses ash, without any germ, there is good water suction to inhale fertilizer, moisturizing supplementary fertilizer through high-temperature sterilization
Effect.
7th, filling substrate
Matrix is fully soaked, is advisable with humidity 60%, or with holding one matrix, is extruded without moisture, hold it is agglomerating,
Touch and then dissipate, matrix is uniformly filled into the hole of seedlings nursing plate.
8th, seeding and growing seedling
Punched in matrix, seed is uniformly sown into the center of cavities, then overall one layer of base of uniform fold on seedlings nursing plate
Matter, and suitably watered according to different ambient humidities.
9th, cold-resistant management
Seed plate after planting is placed in greenhouse, plastic house has plastic sheeting, sunshade net and fly net, carries out daily pipe
Reason；Seedling development is to two leaves wholeheartedly afterwards to foliage-spray plant hormone ABA (abscisic acid), and spraying time is 10 points of morning, concentration
For 10mg/L, it is 1L/4m to squirt dosage2, sprinkling 1 time daily；It is to blade face supplementary fertilizer 1 time, blade face supplementary fertilizer fertilizer daily simultaneously
0.2% potassium dihydrogen phosphate, and a water was sprayed in every 2 days, bed soil water content is kept 73%；Control the control of seedling rearing room day temperature
For 10 DEG C, night temperatures control is 3 DEG C.
10th, garden is gone out
After seedling reaches strong sprout standard, you can transplanted.Seedlings nursing plate was emerged through 2~3 days, after 30~35 day growth phases
Seedling can be transplanted.
It is described above, simply presently preferred embodiments of the present invention, the invention is not limited in above-mentioned embodiment, as long as
It reaches the technique effect of the present invention with identical means, should all belong to protection scope of the present invention.
The experiment activities of antioxidant enzymes expression of the Chinese cabbage cold-resistant of test example 1
Abscisic Acid (ABA) is as " stress hormone " in the winter resistance experienced temperature stress signal, improve plant
In play an important role.The equilibrium condition in plant can be changed using finite concentration Exogenous ABA to induce Cold resistant genes
Expression, so as to strengthen the winter resistance of plant, all shows as, the antioxidase such as superoxide dismutase (SOD) in various plants
Activity improves, and this experiment has done corresponding research to the low temperature source ABA Chinese cabbage winter resistances handled.
Reference examples：For breeding method with the present invention, difference is that spraying distilled water compares；
Experimental example：1-3 breeding methods of the embodiment of the present invention；
Test method：In the Cabbage Seedling cold-resistant stage, it is 10 points of morning to spray the ABA times, sprayed dose substantially 1L/4m2,
The 8th day after spray, sample 3 times within the 12nd day, the 20th day, same area blade is taken when -1 DEG C of 15 DEG C of temperature and temperature
Place and survey index of correlation in laboratory is taken back in ice chest.
Index determining method：The measure of physiological and biochemical index, super oxygen are carried out according to plant physiology experiment guidance method
Thing mutase (SOD) is adopted using NBT photoreduction met hods, peroxidase (POD) using guaiacol method, catalase (CAT)
Use ultraviolet absorption method.
Result of the test is shown in Table 1.
The Chinese cabbage cold-resistant of table 1 tests activities of antioxidant enzymes expression of results
Experiment shows, in the sprouting stage of two leaf of Cabbage Seedling one, either under normal temperature (15 DEG C) or low temperature (- 1 DEG C) state, spray
Winter rape SOD activity can be improved by applying ABA, spray ABA at normal temperatures, and tri- kinds of protective enzymes of SOD, POD and CAT spray compared with distilled water
Apply lower presentation significant difference；ABA is sprayed under low temperature stress, tri- kinds of protective enzymes of SOD, POD and CAT spray lower presentation compared with distilled water
Highly significant difference, after illustrating that Chinese cabbage seedling sprays ABA in low temperature, cold-resistant effect is good.
The Chinese cabbage root system effect of vigor of test example 2
Test method：With test example 1.
Root activity determines：Root activity is surveyed with TTC (triphenyltetrazolium chloride) method.Root is shown with TTC also commercial weight
It is vigor.
Result of the test shows that seedlings root vigor before the Chinese cabbage winter, the root system handled at normal temperatures can be significantly improved by spraying ABA
Vigor increased compared with distilled water, but difference is not notable.And influences of the ABA to root activity occur mainly in by low temperature stress it
Afterwards, reduced with the reduction root activity of temperature, but ABA processing can weaken the trend of root activity reduction.
Claims (10)
1. Chinese cabbage cold-resistant method for culturing seedlings under a kind of low temperature stress, it is characterised in that using plant hormone ABA combinations blade face supplementary fertilizer side
Formula carries out cold-resistant management to Cabbage Seedling；First seed plate after planting is placed in greenhouse, daily management is carried out, treats that seedling development arrives
Two leaves are wholeheartedly afterwards to foliage-spray Abscisic Acid, 1 time a day, while daily to blade face supplementary fertilizer 1 time, and sprayed in 2~3 days
Water, bed soil water content is kept 70~75%.
2. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 1, it is characterised in that the plant hormone ABA spraying times are
In 10 points of morning, blade face is sprayed with the ABA solution of 10mg/L concentration, it is 1L/4m to squirt dosage2。
3. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 1, it is characterised in that the blade face supplementary fertilizer material is 0.2% phosphorus
Acid dihydride potassium or 1% brown sugar or 1% white sugar.
4. Chinese cabbage cold-resistant method for culturing seedlings under a kind of low temperature stress, it is characterised in that comprise the following steps：
1) initial point selection：From quality better, the kind that yield is high, disease-resistant；
2) seedlings nursing plate is selected：According to seed type selecting or make seedlings nursing plate；
3) seedlings nursing plate sterilizes：Seedlings nursing plate is cleaned or sterilized；
4) seed disinfection：Seed is carried out disinfection using instantaneous high-temperature；
5) constant temperature vernalization：Seed is put into insulating box vernalization, until seed germination；
6) seedling medium is allocated：Mud, tealeaf residue, rotten mosses ash and organic fertilizer are mixed into seedling medium；
7) filling substrate：Matrix is filled into the hole of seedlings nursing plate；
8) seeding and growing seedling：Punched in matrix, seed is uniformly sown into the center of cavities, then the overall uniform fold on seedlings nursing plate
One layer of matrix, and suitably watered according to different ambient humidities；
9) cold-resistant management：Under certain temperature, cold-resistant and supplementary fertilizer management are carried out to seed plate.
10) garden is gone out：After seedling reaches strong sprout standard, you can transplanted.
5. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that step 3) the seedlings nursing plate sterilization method
For：The residual matrix in seed plate is first removed, is rinsed well, dried with clear water, and is soaked 12 hours or is used with 500 times of liquid of carbendazim
1000 times of liquid of potassium permanganate, which soak 30 minutes, to be sterilized.
6. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that seed disinfection in the step 4), use
Concentration is 0.1~0.2% liquor potassic permanganate, 90 DEG C of sterilization 25~30min of immersion, sterile water wash seed 2~3 times, is obtained
To sterilized seed.
7. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that seed puts insulating box into the step 5)
Time be 2 to 3 days.
8. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that mud, turf in the step 6) matrix
Ash, the ratio of tealeaf residue are 6：3:0.5~1.5.
9. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that seedling rearing room day temperature in the step 9)
Control as 5~15 DEG C, night temperatures control is~1~5 DEG C.
10. Chinese cabbage cold-resistant method for culturing seedlings as claimed in claim 4, it is characterised in that in the step 10), seedlings nursing plate through 2~
Emerge within 3 days, seedling can be transplanted after 30~35 day growth phases.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112400630A (en) * | 2020-11-23 | 2021-02-26 | 安徽农业大学 | Non-heading Chinese cabbage H capable of reducing low-temperature stress2O2And method of MDA content |
| CN113951063A (en) * | 2021-10-12 | 2022-01-21 | 浙江省农业科学院 | Treating fluid for tomato planting and application of treating fluid in cold-resistant planting of tomatoes |
| CN114793532A (en) * | 2022-05-13 | 2022-07-29 | 中国农业科学院油料作物研究所 | Method for improving drought resistance of rape |
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| CN112400630A (en) * | 2020-11-23 | 2021-02-26 | 安徽农业大学 | Non-heading Chinese cabbage H capable of reducing low-temperature stress2O2And method of MDA content |
| CN113951063A (en) * | 2021-10-12 | 2022-01-21 | 浙江省农业科学院 | Treating fluid for tomato planting and application of treating fluid in cold-resistant planting of tomatoes |
| CN114793532A (en) * | 2022-05-13 | 2022-07-29 | 中国农业科学院油料作物研究所 | Method for improving drought resistance of rape |
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