CN107488577A

CN107488577A - A kind of biofermentation circulation mixing arrangement of comprehensive sampling

Info

Publication number: CN107488577A
Application number: CN201710794428.1A
Authority: CN
Inventors: 杜鑫
Original assignee: Individual
Current assignee: Individual
Priority date: 2017-09-06
Filing date: 2017-09-06
Publication date: 2017-12-19

Abstract

The invention discloses a kind of biofermentation of comprehensive sampling to circulate mixing arrangement, including housing, motor, puddler, dividing plate and supporting leg；Dividing plate will be divided into hybrid chamber and sampling cavity in housing；Helical mixing blade is provided with puddler；Bottom paddle is installed on power transmission shaft；Drawing liquid case and floating block are provided with the sampling cavity, flexible pipe is communicated with drawing liquid case, the other end of circulating pump is communicated with circulation pipe, jet pipe is communicated with the end of circulation pipe；Synthetic sampling device has been inserted vertically into the sampling cavity.The present invention, to internal liquid agitation, is made internal liquid mixing abundant, avoids mixing dead angle and bottom precipitation, ensure the uniform of biofermentation by the helical mixing blade and bottom paddle of setting；Synthetic sampling device can be taken out from inside, and the liquid that can be stretched into difference is sampled, and be isolated after sampling, and avoid being contaminated, there is provided sufficient experimental data.

Description

A kind of biofermentation circulation mixing arrangement of comprehensive sampling

Technical field

The present invention relates to a kind of fermented product process equipment, the biofermentation circulation mixing dress of specifically a kind of comprehensive sampling Put.

Background technology

In biofermentation article manufacturing process, fermentation is to influence the important step of its quality, needed for fermentation and fermentation The fermentation for the strain wanted has very high technological requirement, in fermentation production process, the suitable medicament of the needs such as strain therein And oxygen, medicament is mixed with internal media, ensures to be well mixed, uniform fermentation can be realized, ensure product Quality, but existing installation for fermenting internal mix is simple in construction, and the mixing such as medicament can not be made abundant, influence to produce；In addition, , it is necessary to constantly extract internal zymotic fluid while fermentation, analysis is sampled to composition therein, what is often sampled is The sample of a part inside installation for fermenting, because its volume is big, while the stirring of rabbling mechanism is uneven, causes diverse location Difference, sampling are incomplete for composition, it is impossible to comprehensive sampling, it is unfavorable for grasping fermentation time etc., therefore, it is necessary to existing Biological fermentation device be improved.

The content of the invention

It is an object of the invention to provide a kind of biofermentation of comprehensive sampling to circulate mixing arrangement, to solve above-mentioned background The problem of being proposed in technology.

To achieve the above object, the present invention provides following technical scheme：

A kind of biofermentation circulation mixing arrangement of comprehensive sampling, including housing, motor, puddler, dividing plate and supporting leg；The branch Leg is fixed on the bottom of housing, and longitudinal dividing plate is provided with housing, and dividing plate will be divided into hybrid chamber and sampling cavity in housing； Motor is fixedly mounted in case top corresponding to the hybrid chamber, and the output shaft of motor is extend into hybrid chamber straight down, in electricity The output shaft lower end of machine is fixedly connected with vertical puddler, and helical mixing blade is provided with puddler；The dividing plate Vertical power transmission shaft is installed on the central shaft of lower end by bearing, bottom paddle is installed on power transmission shaft, in bottom paddle On be coaxially installed with driven gear, be fixed with driving gear corresponding to driven gear level on puddler, driving gear with Puddler is coaxially disposed, and driving gear engages connection with driven gear；Drawing liquid case and floating block, floating block are provided with the sampling cavity It is fixed on drawing liquid case, flexible pipe is communicated with drawing liquid case, the upper end of flexible pipe is communicated with circulating pump, and circulating pump is fixed on housing On outer wall, the other end of circulating pump is communicated with circulation pipe, jet pipe is communicated with the end of circulation pipe, jet pipe is fixedly mounted on mixed The top of intracavitary is closed, several nozzles are offered on the downside of jet pipe；Synthetic sampling device has been inserted vertically into the sampling cavity.

Further：The synthetic sampling device includes probe tube, sample tap, column spinner, sampling bottle, intercommunicating pore, close Seal, thief hole, closure and rotary handle, column spinner are inserted into probe tube, are coaxially disposed with probe tube, and rotary handle is solid The upper end of column spinner is scheduled on, several horizontal intercommunicating pores are provided with column spinner, are opened corresponding to the intercommunicating pore on probe tube Provided with thief hole, thief hole is connected in sampling bottle, and sampling bottleneck is provided with the top of sampling bottle, is sampled and is stamped sealing on bottleneck Lid, is being provided with the horizontal centre overlapping of axles of sample tap, sample tap and thief hole, institute in probe tube other side corresponding to intercommunicating pore State and be arranged with sealing ring corresponding to intercommunicating pore on column spinner, intercommunicating pore runs through sealing ring.

Further：The lower end of the dividing plate is provided with breach, and hybrid chamber connects with the bottom of sampling cavity.

Further：Feed pipe is communicated with the side wall of the hybrid chamber.

Further：The distance between bottom of the bottom paddle and housing is less than 2mm.

Further：Several intercommunicating pores are offered in the side wall of the drawing liquid case.

Further：The length of the flexible pipe is more than the height of housing.

Further：The intercommunicating pore is provided with 4~6, and uniformly equidistantly distributes.

Compared with prior art, the beneficial effects of the invention are as follows：Helical mixing blade and bottom of the present invention by setting Portion's paddle makes internal liquid mixing abundant internal liquid agitation, and using the drawing liquid case to suspend by the liquid in sampling cavity Body is extracted out, is made the liquid circulation flowing of inside, further the mixed effect inside raising, is avoided mixing dead angle and bottom sinks Form sediment, ensure the uniform of biofermentation, it is ensured that product quality；Synthetic sampling device can take out from inside, and can be to difference The liquid stretched into is sampled, and is isolated after sampling, and avoids being contaminated, there is provided sufficient experimental data, make work Personnel more accurately grasp the internal environment of biofermentation, and security service is provided for production, and device is easy to operate, sampling peace Entirely, human body will not contact with internal liquid, ensure pure interior, support personnel's safety.

Brief description of the drawings

Fig. 1 is that a kind of biofermentation of comprehensive sampling circulates the structural representation of mixing arrangement.

Fig. 2 is that a kind of biofermentation of comprehensive sampling circulates the structural representation of synthetic sampling device in mixing arrangement.

Fig. 3 is the structural representation of A-A in Fig. 2.

In figure：1- housings, 2- motors, 3- puddlers, 4- helical mixing blades, 5- dividing plates, 6- hybrid chambers, 7- sampling cavities, 8- feed pipes, 9- driving gears, 10- driven gears, 11- power transmission shafts, 12- bottoms paddle, 13- drawing liquid casees, 14- floating blocks, 15- Flexible pipe, 16- circulating pumps, 17- circulation pipes, 18- jet pipes, 19- probe tubes, 20- sample taps, 21- column spinners, 22- sampling bottles, 23- Intercommunicating pore, 24- sealing rings, 25- thief holes, 26- closures, 27- rotary handles, 28- supporting legs.

Embodiment

Refer to figure, in the embodiment of the present invention, a kind of biofermentation circulation mixing arrangement of comprehensive sampling, including housing 1, Motor 2, puddler 3, dividing plate 5 and supporting leg 28；The supporting leg 28 is fixed on the bottom of housing 1, for supporing shell 1, in housing 1 Longitudinal dividing plate 5 is inside provided with, dividing plate 5 will be divided into hybrid chamber 6 and sampling cavity 7 in housing 1, the lower end of dividing plate 5 is provided with breach, Connect hybrid chamber 6 and the bottom of sampling cavity 7；Motor 2 is fixedly mounted in the top of housing 1 corresponding to the hybrid chamber 6, motor 2 Output shaft is extend into hybrid chamber 6 straight down, and vertical puddler 3 is fixedly connected with the output shaft lower end of motor 2, stirring The lower end of bar 3 is fixed by bearing and support bar, and helical mixing blade 4 is provided with puddler 3, in hybrid chamber 6 Liquid is stirred, and feed pipe 8 is communicated with the side wall of hybrid chamber 6, and medicament or nutrient solution are passed through into hybrid chamber 6, in housing 1 Bottom be communicated with discharge nozzle；Vertical power transmission shaft 11, power transmission shaft are installed on the lower end central shaft of the dividing plate 5 by bearing Bottom paddle 12 is installed, the distance between bottom paddle 12 and bottom of housing 1 are less than 2mm, in bottom stirring on 11 Driven gear 10 is coaxially installed with leaf 12, driving gear 9 is fixed with puddler 3 corresponding to the level of driven gear 10, Driving gear 9 is coaxially disposed with puddler 3, and driving gear 9 engages connection with driven gear 10, is rotated puddler 3 and is driven biography Moving axis 11 is rotated, and the bottom of housing 1 is stirred.

Drawing liquid case 13 and floating block 14 are provided with the sampling cavity 7, floating block 14 is fixed on drawing liquid case 13, the He of drawing liquid case 13 Floating block 14 is suspended in internal liquid surface, and several intercommunicating pores are offered in the side wall of drawing liquid case 13, by the liquid in sampling cavity 7 It is pumped into drawing liquid case 13, flexible pipe 15 is communicated with drawing liquid case 13, the length of flexible pipe 15 is more than the height of housing 1, flexible pipe 15 Upper end be communicated with circulating pump 16, circulating pump 16 is fixed on the outer wall of housing 1, and the other end of circulating pump 16 is communicated with circulation Pipe 17, is communicated with jet pipe 18 on the end of circulation pipe 17, and jet pipe 18 is fixedly mounted on the top in hybrid chamber 6, under jet pipe 18 Side offers several nozzles, and internal liquid uniformly is sprayed, and is mixed in hybrid chamber 6.

Synthetic sampling device has been inserted vertically into the sampling cavity 7, and has passed through the top of the upper lid with sealing ring and housing 1 Portion is tightly connected, and synthetic sampling device includes probe tube 19, sample tap 20, column spinner 21, sampling bottle 22, intercommunicating pore 23, close Seal 24, thief hole 25, closure 26 and rotary handle 27, column spinner 21 are inserted into probe tube 19, coaxial with probe tube 19 Set, rotary handle 27 is fixed on the upper end of column spinner 21, rotates column spinner 21 by rotary handle 27, becomes its angle Change, several horizontal intercommunicating pores 23 are provided with column spinner 21, intercommunicating pore 23 is provided with 4~6, and uniformly equidistant point Cloth, thief hole 25 is offered corresponding to the intercommunicating pore 23 on probe tube 19, thief hole 25 is connected in sampling bottle 22, is being sampled The top of bottle 22 is provided with sampling bottleneck, samples and is stamped closure 26 on bottleneck, in probe tube corresponding to intercommunicating pore 23 19 other one Side is provided with the horizontal centre overlapping of axles of sample tap 20, sample tap 20 and thief hole 25, and sample tap 20 is in sampling cavity 7 and internal Fluid connection, liquid is entered from sample tap 20, sealing ring 24 is arranged with column spinner 21 corresponding to the intercommunicating pore 23, connect Sealing ring 24 is run through in hole 23, and sealing ring 24 makes to seal between column spinner 21 and probe tube 19, avoids the liquid of upper and lower diverse location Mixing；In use, rotating column spinner 21 by rotary handle 27, intercommunicating pore 23 is set to turn to different angles, when needing to sample Mouthfuls 20 with sampling bottle 22 is interior connect when, intercommunicating pore 23 is turned into the same horizontal position, when liquid enter from sample tap 20, pass through Intercommunicating pore 23 and thief hole 25 are entered in sampling bottle 22.

Nutrient solution is distributed in housing 1 in hybrid chamber 6 and sampling cavity 7, when need to nutrient solution addition medicament carry out During mixing, start motor 2, motor 2 drives puddler 3 to rotate, and drives internal agitation by helical mixing blade 4 thereon, makes Medicament entered in feed pipe 8 etc. mixes with nutrient solution, while the engaged transmission of driving gear 9 of the lower end of puddler 3 makes biography Moving axis 11 is rotated, and the nutrient solution of the bottom of housing 1 is stirred, mixing is stirred again to the nutrient solution for flowing through the lower end of dividing plate 5, takes Drawing liquid case 13 in sample chamber 7 is suspended on liquid level, and upside liquid is extracted out, and is sent to by circulation pipe 17 in jet pipe 18, Nutrient solution is set uniformly to spray again, so that internal liquid circulation flowing, makes medicament mixing full and uniform；When needs pair During the sampling of inside, the rotary handle 27 in synthetic sampling device is rotated, rotates connected column spinner 21, intercommunicating pore 23 turns to Towards on the direction of sample tap 20, the liquid for making to enter in sample tap 20 enters sampling by intercommunicating pore 23 and thief hole 25 In bottle 22, sample after terminating, then column spinner 21 is rotated backward, can be so that by synthetic sampling device, from inside, proposition is upwards Can, make carrying out experimental analysis, experiment can directly be extracted by opening the closure 26 of sampling bottle 22.The spiral shell that the present invention passes through setting Stirring vane and bottom paddle are revolved to internal liquid agitation, make internal liquid mixing abundant, and utilize the drawing liquid to suspend Case extracts the liquid in sampling cavity out, makes the liquid circulation flowing of inside, further the mixed effect inside raising, is avoided mixed Dead angle and bottom precipitation are closed, ensures the uniform of biofermentation, it is ensured that product quality；Synthetic sampling device can take from inside Go out, and the liquid that can be stretched into difference is sampled, and isolated after sampling, avoid being contaminated, there is provided sufficient Experimental data, staff is more accurately grasped the internal environment of biofermentation, for production security service, device are provided Easy to operate, sampling safety, human body will not contact with internal liquid, ensure pure interior, support personnel's safety.

Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It is appreciated that other embodiment.

Claims

1. a kind of biofermentation circulation mixing arrangement of comprehensive sampling, including housing（1）, motor（2）, puddler（3）, dividing plate （5）And supporting leg（28）；It is characterized in that：The supporting leg（28）It is fixed on housing（1）Bottom, in housing（1）Inside it is provided with vertical To dividing plate（5）, dividing plate（5）By housing（1）Inside it is divided into hybrid chamber（6）And sampling cavity（7）；The hybrid chamber（6）It is corresponding Housing（1）Motor is fixedly mounted in top（2）, motor（2）Output shaft extend into hybrid chamber straight down（6）It is interior, in motor（2） Output shaft lower end be fixedly connected with vertical puddler（3）, in puddler（3）On be provided with helical mixing blade（4）；It is described Dividing plate（5）Lower end central shaft on vertical power transmission shaft is installed by bearing（11）, power transmission shaft（11）On bottom be installed stirred Mix leaf（12）, in bottom paddle（12）On be coaxially installed with driven gear（10）, driven gear（10）Corresponding to level Puddler（3）On be fixed with driving gear（9）, driving gear（9）With puddler（3）It is coaxially disposed, driving gear（9）With it is driven Gear（10）Engagement connection；The sampling cavity（7）Inside it is provided with drawing liquid case（13）And floating block（14）, floating block（14）It is fixed on drawing liquid Case（13）On, in drawing liquid case（13）Inside it is communicated with flexible pipe（15）, flexible pipe（15）Upper end be communicated with circulating pump（16）, circulating pump （16）It is fixed on housing（1）Outer wall on, circulating pump（16）Other end be communicated with circulation pipe（17）, circulation pipe（17）End Jet pipe is communicated with end（18）, jet pipe（18）It is fixedly mounted on hybrid chamber（6）Interior top, jet pipe（18）If downside offer Dry nozzle；The sampling cavity（7）Inside it has been inserted vertically into synthetic sampling device.

A kind of 2. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：It is described comprehensive Conjunction sampling device includes probe tube（19）, sample tap（20）, column spinner（21）, sampling bottle（22）, intercommunicating pore（23）, sealing ring （24）, thief hole（25）, closure（26）And rotary handle（27）, column spinner（21）It is inserted into probe tube（19）It is interior, with sampling Pipe（19）It is coaxially disposed, rotary handle（27）It is fixed on column spinner（21）Upper end, column spinner（21）On be provided with several water Flat intercommunicating pore（23）, the intercommunicating pore（23）Corresponding probe tube（19）On offer thief hole（25）, thief hole（25）Even Lead in sampling bottle（22）It is interior, in sampling bottle（22）Top be provided with sampling bottleneck, sample bottleneck on be stamped closure（26）, even Through hole（23）Corresponding probe tube（19）Other side is provided with sample tap（20）, sample tap（20）With thief hole（25）Level Center overlapping of axles, the intercommunicating pore（23）Corresponding column spinner（21）On be arranged with sealing ring（24）, intercommunicating pore（23）Through close Seal（24）.

A kind of 3. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：It is described every Plate（5）Lower end be provided with breach, hybrid chamber（6）And sampling cavity（7）Bottom connection.

A kind of 4. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：It is described mixed Close chamber（6）Side wall on be communicated with feed pipe（8）.

A kind of 5. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：The bottom Portion's paddle（12）With housing（1）The distance between bottom be less than 2mm.

A kind of 6. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：It is described to take out Liquid case（13）Side wall on offer several intercommunicating pores.

A kind of 7. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 1, it is characterised in that：It is described soft Pipe（15）Length be more than housing（1）Height.

A kind of 8. biofermentation circulation mixing arrangement of comprehensive sampling according to claim 2, it is characterised in that：The company Through hole 23 is provided with 4~6, and uniformly equidistantly distributes.