CN107485577A - Active peptides human-like collagen CH - Google Patents
Active peptides human-like collagen CH Download PDFInfo
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- CN107485577A CN107485577A CN201710694185.4A CN201710694185A CN107485577A CN 107485577 A CN107485577 A CN 107485577A CN 201710694185 A CN201710694185 A CN 201710694185A CN 107485577 A CN107485577 A CN 107485577A
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- Prior art keywords
- collagen
- reaction
- albumen
- skin
- active peptides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0024—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Glucans; (beta-1,3)-D-Glucans, e.g. paramylon, coriolan, sclerotan, pachyman, callose, scleroglucan, schizophyllan, laminaran, lentinan or curdlan; (beta-1,6)-D-Glucans, e.g. pustulan; (beta-1,4)-D-Glucans; (beta-1,3)(beta-1,4)-D-Glucans, e.g. lichenan; Derivatives thereof
- C08B37/0027—2-Acetamido-2-deoxy-beta-glucans; Derivatives thereof
- C08B37/003—Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08G—MACROMOLECULAR COMPOUNDS OBTAINED OTHERWISE THAN BY REACTIONS ONLY INVOLVING UNSATURATED CARBON-TO-CARBON BONDS
- C08G81/00—Macromolecular compounds obtained by interreacting polymers in the absence of monomers, e.g. block polymers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/95—Involves in-situ formation or cross-linking of polymers
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Birds (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Materials Engineering (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a kind of active peptides human-like collagen CH, it comprises the following steps：Step 1, prepare 0.1M MES (2 morpholino b acid), 0.5M NaCL (sodium chloride), pH5.5 cushioning liquid 100ml, collagen 200mg is added, adds certain density EDC: NHS=4: 1,10 DEG C are maintained the temperature at, is reacted 1.5 hours；Step 2, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak；Step 3, configuration 0.1M PBS (vulcanized lead), 0.15M NaCL (sodium chloride), pH 7.2 cushioning liquid 100ml etc..The barrier function of present invention enhancing skin, reduces the evaporation of skin internal moisture, while resist damage of the extraneous factors such as ultraviolet, dust, haze to skin.
Description
Technical field
The present invention relates to a kind of cosmetics, more particularly to a kind of active peptides-human-like collagen-CH.
Background technology
Hyaluronic acid is also known as uronic acid, sodium hyaluronate, higher polysaccharides class, is a kind of acid mucopolysaccharide.U.S. brother in 1934
Rival Asia university ophthalmology professor Mayer etc. isolates the material from bovine vitreous body first.Hyaluronic acid is skin and other
The bio-molecules being widely present in tissue, it is referred to as preferable moisturizing factor in the world.Be present in connective tissue such as joint,
Among vitreum, synovia, umbilical cord, cartilage, skin, cockscomb, to meet some critical functions such as toughness, support structure and cell
Metabolism regulation.Hyaluronic acid in skin occupies the 50% of the total hyaluronic acid of body, and the hyaluronic acid contents in corium show
Write and be higher than epidermis, closely, the utilization in cosmetics is also more and more extensive for the relation of hyaluronic acid and skin, especially special
Different water retention, it is the material that moisture retention is best in the nature having now been found that, is referred to as preferable NMF.
Human skin is ripe and ageing process changes also with content and the metabolism of hyaluronic acid.It can improve
Skin-nourishing is metabolized, make skin it is tender, it is smooth, go to wrinkle, increase it is elastic, prevent aging, be good saturating again while moisturizing
Skin sorbefacient.It is used cooperatively with other nutritional ingredients, the more preferable effect for promoting nutrient absorption can be played.
Contain substantial amounts of carboxyl and hydroxyl in hyaluronan molecule, form intramolecular and intermolecular hydrogen in aqueous
Key, this makes it have powerful water retention, can combine itself more than 400 times of water；Macromolecular hyaluronic acid to signaling,
Propagation, differentiation and phagocytic function have inhibitory action, and micromolecule hyaluronic acid then has facilitation.In higher concentration, due to it
The complicated tridimensional network that intermolecular interaction is formed, the aqueous solution have significant viscoplasticity again.
Hyaluronic acid also has obvious influence on effect because of raw material sources and the difference of manufacturing technology.Purity, molecular weight,
3D stereochemical structures can all directly affect the soaking effect of hyaluronic acid.
Macromolecular hyaluronic acid (molecular weight ranges 1 80 ten thousand~2,200,000) can form one layer ventilative thin in skin surface
Film, skin smooth is moistened, and the intrusion of alien bacteria, dust, ultraviolet can be obstructed, protect the skin from encroaching on.Middle molecule
Hyaluronic acid (molecular weight ranges 1 00 ten thousand~1 80 ten thousand) can compact skin, permanent moisturizing.Micromolecule hyaluronic acid (molecular weight
Ten thousand) scope 400,000~1 00 can penetrate into corium, have mild dilation capillary, increase blood circulation, improve intermediate supersession, promote
Enter skin-nourishing absorption, there is stronger wrinkle-chasing function, skin elasticity, delay skin aging can be increased.
It is using the hyaluronic acid composition containing three kinds of different molecular weights as criterion, only to weigh a good skin care item
Skin care item effect containing unimodal molecular weight hyaluronic acid composition is limited, can not play the optimal skin-care effect of hyaluronic acid.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of active peptides-human-like collagen-CH, and it passes through cosmetic
The mode of product addition, the super high molecular weight polymer obtained by Sodium Hyaluronate by cross-linking reaction, can be formed in skin surface
A kind of special 3 D stereo network structure, skin surface is uniformly covered on, forms a kind of ventilative diaphragm, strengthen skin
Barrier function, reduce the evaporation of skin internal moisture, while resist ultraviolet, dust, the extraneous factor such as haze is to skin
Damage.
The present invention is that solve above-mentioned technical problem by following technical proposals：A kind of active peptides-class people's collagen egg
- CH in vain, it comprises the following steps：
Step 1, prepare 0.1M MES (MES), 0.5M NaCL (sodium chloride), pH5.5 cushioning liquid
100ml, collagen 200mg is added, adds certain density EDC: NHS=4: 1, maintain the temperature at 10 DEG C, reaction 1.5 is small
When；
Step 2, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak；
Step 3, configuration 0.1M PBS (vulcanized lead), 0.15M NaCL (sodium chloride), pH 7.2 cushioning liquid 100ml,
The activated protein of step 2 concentration gained is added, reaction system protein concentration reaches 10mg/ml, adds the transparent of certain percentage
Matter acid sodium, 18 DEG C are maintained the temperature at, react 4-6 hours；
Step 4, the product physics and chemistry index of measure reaction gained.
Preferably, the step 1 comprises the following steps：
Step 11, albumen is first activated on Sodium Hyaluronate is coupled to, this activator for using of experiment for
EDC ((1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides)；
Step 12, water miscible carbodiimide hydrochloride come the carboxyl group of modified protein molecule, have been one at present
Kind most common standard method, it can will it is gentle under conditions of carry out；
Step 13, need to be commonly incorporated into NHS-MAG3 (Mercaptoacetyl triglycine n-hydroxysuccinimides in reaction
Ester) this intermediate is become stable, while yield is improved, reduce the generation of side reaction；
Step 14, the albumen of selection is collagen.In order to keep the activity of albumen, reaction need to be in pH4-6 buffering
Carried out in solution.
Preferably, the step 2 comprises the following steps：
Step 2 15, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak.
Preferably, the step 3 comprises the following steps：
Step 3 11, the 0.1M PBS (vulcanized lead) of configuration, 0.15M NaCL (sodium chloride), pH 7.2 buffering is molten
Liquid 100ml, it is added in the albumen wash-out peak that reaction solution is obtained using chromatography.
The positive effect of the present invention is：The present invention is passed through by way of cosmetics addition by Sodium Hyaluronate
The super high molecular weight polymer that cross-linking reaction obtains, a kind of special 3 D stereo network structure can be formed in skin surface,
Even is covered in skin surface, forms a kind of ventilative diaphragm, strengthens the barrier function of skin, reduces skin internal moisture
Evaporation, while resists ultraviolet, dust, the damage of the extraneous factor to skin such as haze.
Embodiment
Present pre-ferred embodiments are given below, to describe technical scheme in detail.
Active peptides of the present invention-human-like collagen-CH comprises the following steps：
Step 1, prepare 0.1M MES (MES), 0.5M NaCL (sodium chloride), pH5.5 cushioning liquid
100ml, collagen 200mg is added, adds certain density EDC: NHS=4: 1, maintain the temperature at 10 DEG C, reaction 1.5 is small
When；
Step 2, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak；
Step 3, configuration 0.1M PBS (vulcanized lead), 0.15M NaCL (sodium chloride), pH 7.2 cushioning liquid 100ml,
The activated protein of step 2 concentration gained is added, reaction system protein concentration reaches 10mg/ml, adds the transparent of certain percentage
Matter acid sodium, 18 DEG C are maintained the temperature at, react 4-6 hours；
Step 4, the product physics and chemistry index of measure reaction gained.
The step 1 comprises the following steps：
Step 11, albumen is first activated on Sodium Hyaluronate is coupled to, this activator for using of experiment for
EDC ((1- (3- dimethylamino-propyls) -3- ethyl-carbodiimide hydrochlorides)；
Step 12, water miscible carbodiimide hydrochloride come the carboxyl group of modified protein molecule, have been one at present
Kind most common standard method, it can will it is gentle under conditions of carry out；
Step 13, need to be commonly incorporated into NHS-MAG3 (Mercaptoacetyl triglycine n-hydroxysuccinimides in reaction
Ester) this intermediate is become stable, while yield is improved, reduce the generation of side reaction；
Step 14, the albumen of selection is collagen.In order to keep the activity of albumen, reaction need to be in pH4-6 buffering
Carried out in solution.
The step 2 comprises the following steps：
Step 2 15, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak.
The step 3 comprises the following steps：
Step 3 11, the 0.1M PBS (vulcanized lead) of configuration, 0.15M NaCL (sodium chloride), pH 7.2 buffering is molten
Liquid 100ml, it is added in the albumen wash-out peak that reaction solution is obtained using chromatography.
In summary, the present invention is surpassed by way of cosmetics addition by Sodium Hyaluronate by what cross-linking reaction obtained
Heavy polymer, a kind of special 3 D stereo network structure can be formed in skin surface, is uniformly covered on skin table
Face, a kind of ventilative diaphragm is formed, strengthen the barrier function of skin, reduce the evaporation of skin internal moisture, while resist purple
Damage of the extraneous factors such as outside line, dust, haze to skin.
Particular embodiments described above, technical problem, technical scheme and the beneficial effect of the solution to the present invention are carried out
It is further described, should be understood that the specific embodiment that the foregoing is only of the invention, be not limited to
The present invention, within the spirit and principles of the invention, any modification, equivalent substitution and improvements done etc., it should be included in this
Within the protection domain of invention.
Claims (4)
1. a kind of active peptides-human-like collagen-CH, it is characterised in that it comprises the following steps：
Step 1,0.1M MES, 0.5M NaCL, pH5.5 cushioning liquid 100ml are prepared, add collagen 200mg, then add
Enter certain density EDC: NHS=4: 1, maintain the temperature at 10 DEG C, react 1.5 hours；
Step 2, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak；
Step 3, configuration 0.1M PBS, 0.15M NaCL, pH 7.2 cushioning liquid 100ml, add step 2 concentration gained
Activated protein, reaction system protein concentration reach 10mg/ml, add the Sodium Hyaluronate of certain percentage, maintain the temperature at 18
DEG C, react 4-6 hours；
Step 4, the product physics and chemistry index of measure reaction gained.
2. active peptides as claimed in claim 1-human-like collagen-CH, it is characterised in that the step 1 includes following
Step：
Step 11, albumen is first activated on Sodium Hyaluronate is coupled to, the activator that this experiment uses is EDC；
Step 12, water miscible carbodiimide hydrochloride come the carboxyl group of modified protein molecule, be at present it is a kind of most
Universal standard method, it can be carried out under conditions of will be gentle；
Step 13, needing to be commonly incorporated into NHS-MAG3 in reaction makes this intermediate become stable, while improves yield, reduces secondary
The generation of reaction；
Step 14, the albumen of selection is collagen；In order to keep the activity of albumen, reaction need to be in pH4-6 cushioning liquid
Middle progress.
3. active peptides as claimed in claim 1-human-like collagen-CH, it is characterised in that the step 2 includes following
Step：
Step 2 15, reaction solution obtained by step 1 is used into chromatography, crosses G25 gels, collects albumen wash-out peak.
4. active peptides as claimed in claim 1-human-like collagen-CH, it is characterised in that the step 3 includes following
Step：
Step 3 11, by 0.1M PBS, the 0.15M NaCL, pH 7.2 of configuration cushioning liquid 100ml, it is molten to be added to reaction
In the albumen wash-out peak that liquid is obtained using chromatography.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710694185.4A CN107485577A (en) | 2017-08-15 | 2017-08-15 | Active peptides human-like collagen CH |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710694185.4A CN107485577A (en) | 2017-08-15 | 2017-08-15 | Active peptides human-like collagen CH |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662926A (en) * | 2019-01-29 | 2019-04-23 | 广州锦同生物科技有限公司 | A kind of polywater moisturizing essence dew and preparation method thereof |
-
2017
- 2017-08-15 CN CN201710694185.4A patent/CN107485577A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109662926A (en) * | 2019-01-29 | 2019-04-23 | 广州锦同生物科技有限公司 | A kind of polywater moisturizing essence dew and preparation method thereof |
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