CN1074800A - Fast virucide for AIDS virus - Google Patents

Abstract

A kind of fast virucide for AIDS virus of forming by five carbon bisacetals, dodecyl benzyl dimethyl ammonium bromide, T-peptide, α-butyrolactone, ethanol, deionized water.Wherein two of five carbon bisacetals active aldehydes can react with protein, and T-peptide and α-butyrolactone can increase its deactivation ability greatly, simultaneously energy quick inactivating pericentral siphon enzyme, alkaline phosphatase and cellular enzymes.Fast virucide of the present invention has that the AIDS virus of killing speed is fast, germicidal efficiency is high, and required dosage is little, and nontoxic advantage.

Description

Fast virucide for AIDS virus

The present invention relates to a kind of bactericidal agent, particularly to the fast virucide of AIDS virus.

The Chinese patent name be called " liquid cleaning and sanitizing agent ", application number be 88100230 and name be called " solid hepatitis and AIDS virus Efficacious Disinfeitant ", application number is 89102226 to disclose a kind of decontaminant and a kind of Efficacious Disinfeitant respectively, the former mainly adopts clorox, the latter then adopts chlorinated sodium phosphate to make bactericide, and they are chlorinated product.Separate and TPPA according to AIDS virus, HIV is the pathogeny of acquired immune deficiency syndrome (AIDS).HIV belongs to the reverse transcription RNA viruses, and form is typical D type virus, and eccentric nuclear is arranged, and revertase needs Mg ⁺⁺Ion, main core protein biological property is to have a liking for the T4 lymphocyte.Cell forms fusion cell at a large amount of releasing virus after week of 1-2 behind the infective virus, and last cell is destroyed.Viral nucleic acid HIV virus is people's retrovirus, and the viral gene of three standards is arranged, and further molecular biology research is found, HIV has very big genetic variation, comprises some variation, disappearance or the insertion of nucleic acid.The envelope protein of cellulosa (GP 120) is easy to change, and determines its deactivation degree by reverse transcriptase activity.Adopting chlorinated product to kill the AIDS virus weak point is that to kill speed slow, and kill efficiency is low, and required dosage is big, and has little poison.

The objective of the invention is to avoid above-mentioned the deficiencies in the prior art part, provide a kind of poisoning speed of going out fast, the kill efficiency height, required dosage is little, and nontoxic fast virucide for AIDS virus.

Fast virucide for AIDS virus of the present invention is made up of five carbon bisacetals, dodecyl benzyl dimethyl ammonium bromide, T-peptide, α-butyrolactone, ethanol and deionized water, and its percentage by weight is:

Five carbon bisacetals 1.0～2.5

Dodecyl benzyl dimethyl ammonium bromide 2.8～5.5

T-peptide 0.15～0.4

Ethanol (95%) 15.0～35.0

Deionized water 60.0～80.0

Purpose of the present invention can reach by the following method:

A. dodecyl benzyl dimethyl ammonium bromide is added in the ethanol (95%), be warming up to 45 ± 2 ℃, stirring and dissolving 1 hour;

B. T-peptide, α-butyrolactone are dissolved in the deionized water, add five carbon bisacetals again, dissolving fully;

C. the aqueous solution that obtains of ethanolic solution that above-mentioned a is obtained and above-mentioned b is even, measures its pH value to 6～7 and gets final product.

Five carbon bisacetals are a kind of new disinfectant, and two aldehyde radicals in its structure can react with the amino of bacterial cell, the outer sealing process that rises of pair cell, and energy as killed cells enzyme, thus reach sterilization functions.Dodecyl benzyl dimethyl ammonium bromide is a kind of cationic surfactant, because of its surface-active ion that carries positive charge, bacteriostasis is arranged.α-butyrolactone has the α-butyrolactone that adds trace in the bactericidal action of blood plasma vaccine, the fast virucide can increase its deactivation ability greatly.The T-peptide is a kind of compound that has amide group, is the intermediate product of proteolysis, is called peptide, and bacteriostasis is arranged.The formulated fast virucide of these several components has extremely strong deactivation ability, energy deactivation pericentral siphon enzyme, alkaline phosphatase and cellular enzymes, thus reach sterilization functions fast.

The invention will be further described below in conjunction with embodiment: each component of the listed data decimation of according to the form below, prepare in 1000 liters of stainless steel cauldrons with said method.

The component title	Weight (Kg)
					Embodiment 1	Embodiment 2	Embodiment 3	Embodiment 4
	Five carbon bisacetal dodecyl benzyl dimethyl ammonium bromide T-peptide α-butyrolactone ethanol (95%) deionized waters	1.4 5.0 0.2 0.04 21.0 72.36	1.3 4.4 0.24 0.03 24.5 69.03	2.0 4.0 0.3 0.05 27.0 66.65					2.2 3.5 0.4 0.02 30.38 63.5

It below is the result of the test of fast virucide for AIDS virus inactivating AIDS poison (HIV) effect.

One, virus infections test

Be subjected to examination virus to be HIV BA-2 strain, in the 50ul viral suspension, add 1ml MT4 cell suspension during test, room temperature absorption is after 40 minutes, 2000 rev/mins, centrifugation cell, with the cell that 200ul RPMI1640 medium suspends and precipitates, on 96 hole microtest plates, use MT4 cell titration virus titer.Every dilution factor virus is done four holes,, presses the Reed-Muench method and calculates TCID50 as the virus infections index with cytopathy.

Two, neutralizer test

Testing all neutralizers is the phosphate buffer that contains 1% glycine+0.5% Tween 80, with following eight groups of test examination neutralization, triplicate.

1.(fast virucide+virus)+1: 3 fast virucide of neutralizer: 50ul and 50ul viral suspension immixture be after 1 minute, adds the 50ul neutralizer, and 30 minutes.

2. neutralizer+virus: 50ul neutralizer and 50ul viral suspension immixture 30 minutes.

3.(fast virucide+neutralizer)+1: 3 fast virucide of virus: 50ul and 50ul neutralizer immixture be after 30 minutes, again with 50ul viral suspension effect 1 minute.

4. 1: 3 fast virucide of fast virucide+virus: 50ul and 50ul viral suspension immixture are 1 minute.

More than after four groups of effects by method one titration virus infections titre.

5. virus control: undertaken by method one.

6.(fast virucide+neutralizer)+and 1: 3 fast virucide of cell: 50ul and 50ul neutralizer immixture be after 30 minutes, adds the MT4 cell suspension, cultivate by method one titration after 40 minutes.

7. neutralizer+cell: 50ul neutralizer and 1ml MT ₄Mixing with cells was cultivated by method one titration after 40 minutes.

8. cell contrast: cell in the culture hole does not add virus.

Three, inactivation of virus test

Get the fast virucide of 50ul variable concentrations and 50ul viral suspension immixture after one minute, add the 50ul neutralizer, neutralization 30 minutes, detect the virus infections titre by method one, set up the virus control and the normal cell contrast of not deactivation simultaneously, repeat five times, be calculated as follows inactivation ratio:

Inactivation ratio (%)=(average virus infections titre is organized in the average virus infections titre-sterilization of control group)/(the average virus infections titre of control group) * 100%

Testing result:

Table 1: neutralizer result of the test

The average infection titer of group (TCID50)

1 1.233

2 4.872

3 4.970

4 0.874

5 5.242

As seen from Table 1, neutralizer can in and fast virucide to the deactivation of virus, neutralizer and neutralized reaction product do not have obvious inhibitory action to virus.In addition, neutralizer and neutralized reaction product do not have overt toxicity to cultured cell, cell contrast well-grown.

Table 2: fast virucide is to the inactivating efficacy of HIV

The average inactivation ratio of fast virucide dilution factor (%)

1:1 100

1:3 99.99

1:9 99.37

Table 2 presentation of results, virus did not see and cause cytopathy that the fast virucide of dilution in 1: 3 can not reach the sterilization requirement in 1 minute with HIV deactivation 99.99%, 1: 9 dilution fast virucide effects in 1 minute in one minute with the effect of fast virucide stoste.

Acute toxicity experience:

Mouse LD ₅₀=11071.3mg/kg

Eye stimulates and skin patch test: to tame rabbit acute eye stimulating separate index number (peak)=4; It is 0 that local skin stimulates integration.Conclusion is: fast virucide of the present invention belongs to nontoxic product.To the basic nonirritant of lagophthalmos, rabbit skin is had no stimulation.

Fast virucide of the present invention has that the AIDS virus of killing speed is fast, germicidal efficiency is high, and required dosage is little, and nontoxic advantage.

Claims (2)

1, a kind of fast virucide for AIDS virus is characterized in that being made up of five carbon bisacetals, dodecyl benzyl dimethyl ammonium bromide, T-peptide, α-butyrolactone, ethanol and deionized water, and its percentage by weight is:

Five carbon bisacetals 1.0～2.5

Dodecyl benzyl dimethyl ammonium bromide 2.8～5.5

T-peptide 0.15～0.4

α-butyrolactone 0.02～0.5

Ethanol (95%) 15.0～35.0

Deionized water 60.0～80.0

2, fast virucide for AIDS virus according to claim 1 is characterized in that:

A. dodecyl benzyl dimethyl ammonium bromide is added in the ethanol (95%), be warming up to 45 ± 2 ℃, stirring and dissolving 1 hour;

B. T-peptide, α-butyrolactone are dissolved in the deionized water, add five carbon bisacetals again, dissolving fully;

C. the aqueous solution that obtains of ethanolic solution that above-mentioned a is obtained and above-mentioned b is even, measures its pH value to 6～7 and gets final product.