CN107476778B - It is a kind of to adjust technique using microorganism and the compound deep of clay gel are stifled - Google Patents
It is a kind of to adjust technique using microorganism and the compound deep of clay gel are stifled Download PDFInfo
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- 239000004927 clay Substances 0.000 title claims abstract description 84
- 244000005700 microbiome Species 0.000 title claims abstract description 48
- 238000000034 method Methods 0.000 title claims abstract description 34
- 150000001875 compounds Chemical class 0.000 title claims abstract description 15
- 238000002347 injection Methods 0.000 claims abstract description 74
- 239000007924 injection Substances 0.000 claims abstract description 74
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 72
- 238000012360 testing method Methods 0.000 claims abstract description 60
- 239000003513 alkali Substances 0.000 claims abstract description 55
- 239000011259 mixed solution Substances 0.000 claims abstract description 55
- 239000000243 solution Substances 0.000 claims abstract description 53
- 238000000855 fermentation Methods 0.000 claims abstract description 45
- 230000004151 fermentation Effects 0.000 claims abstract description 45
- 238000004519 manufacturing process Methods 0.000 claims abstract description 25
- 238000012216 screening Methods 0.000 claims abstract description 19
- 230000000813 microbial effect Effects 0.000 claims abstract description 18
- 230000007423 decrease Effects 0.000 claims abstract description 11
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 230000035699 permeability Effects 0.000 claims description 59
- 239000011435 rock Substances 0.000 claims description 40
- 239000007788 liquid Substances 0.000 claims description 30
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 23
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 19
- 238000005259 measurement Methods 0.000 claims description 15
- 241000588986 Alcaligenes Species 0.000 claims description 14
- 241000252867 Cupriavidus metallidurans Species 0.000 claims description 14
- 238000003756 stirring Methods 0.000 claims description 14
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims description 12
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 12
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 12
- 238000011068 loading method Methods 0.000 claims description 10
- 239000004576 sand Substances 0.000 claims description 9
- 235000015097 nutrients Nutrition 0.000 claims description 7
- 239000000375 suspending agent Substances 0.000 claims description 7
- 229920002472 Starch Polymers 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 238000011049 filling Methods 0.000 claims description 5
- 230000003647 oxidation Effects 0.000 claims description 5
- 238000007254 oxidation reaction Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 4
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 4
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 4
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 4
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 238000009738 saturating Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000003247 decreasing effect Effects 0.000 abstract description 3
- 239000003129 oil well Substances 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 42
- 239000010410 layer Substances 0.000 description 7
- 239000002981 blocking agent Substances 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 230000036571 hydration Effects 0.000 description 5
- 238000006703 hydration reaction Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 206010042674 Swelling Diseases 0.000 description 4
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical group O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 229910052901 montmorillonite Inorganic materials 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 239000009671 shengli Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- ZGINPHARJIMXMG-UJPDDDSFSA-N [O].OC[C@@H](O)[C@H](O)[C@@H](O)C=O Chemical compound [O].OC[C@@H](O)[C@H](O)[C@@H](O)C=O ZGINPHARJIMXMG-UJPDDDSFSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 230000000903 blocking effect Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B33/00—Sealing or packing boreholes or wells
- E21B33/10—Sealing or packing boreholes or wells in the borehole
- E21B33/13—Methods or devices for cementing, for plugging holes, crevices or the like
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/20—Displacing by water
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- Geology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mining & Mineral Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Fluid Mechanics (AREA)
- Physics & Mathematics (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geochemistry & Mineralogy (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cosmetics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention belongs to oil-gas field development technical fields, and in particular to a kind of to adjust technique using microorganism and the compound deep of clay gel are stifled, the technique is specifically includes the following steps: test the screening of oil reservoir；Produce the screening of alkali microorganism；It determines and produces alkali microbial fermentation solution and clay gel ratio；Determine the total injection for producing alkali microbial fermentation solution and clay gel；The preparation of mixed solution；Field test.The present invention has simple process, blocks up the intensity height adjusted, and cost of investment is low, the obvious feature of decreasing water cut and increasing oil effect, and 10% or more oil well comprehensive water cut decline, amount of increase in production is greater than 50%.Therefore, present invention is generally applicable in the stifled tune technique in the deep of medium to high permeable oil reservoir.
Description
Technical field
The invention belongs to oil-gas field development technical fields, and in particular to a kind of stifled using microorganism and the compound deep of clay gel
Adjust technique.
Background technique
Medium to high permeable oil reservoir water flow predominant pathway (macropore) easy to form during long-period water drive is developed, due to oil
The heterogeneity of hiding is serious, reduces the swept volume of waterflooding development, to affect the development effectiveness of water drive.Therefore, it is necessary to
Water plugging and profile controlling processing is carried out, to improve the swept volume of water drive or other displacement technologies, to further increase the crude oil of oil reservoir
Recovery ratio.
Currently, " injection efficiency " contradiction taken into account different from " intensity " is faced with for the stifled blocking agent adjusted, if blocking agent has
There is preferable intensity, then its mobility is with regard to undesirable, it is difficult to oil deposit deep part is injected, on the contrary, if blocking agent has preferable stream
Dynamic property, then its intensity may be inadequate, it is difficult to play the role of preferably blocking up tune.Therefore, to solve it is this " note not into, it is stifled not
Contradiction firmly " needs blocking agent to have preferable mobility in injection, can be injected into oil deposit deep part, when blocking agent reaches oil reservoir depth
It behind portion, can voluntarily change, keep its mobility variations poor, and the intensity blocked becomes larger, and realizes that deep is stifled and adjusts.
Clay gel main component is montmorillonite, due to cheap and be easy to get, is once used for the displacement of reservoir oil, but when using excessive concentration
It (being higher than 5%), although there is enough stifled tune intensity, is difficult to inject, (is lower than 3%) when too low using concentration, although being easy
Injection, but stifled tune intensity is inadequate.Using concentration there is certain oil displacement efficiency in 3%~5% range, but still has a large amount of
Clay gel can not achieve " deep " stifled tune truly from producing well output.
Clay glue host is montmorillonite, montmorillonite water swelling, and mechanism of intumescence can be divided into surface hydration expansion and infiltration
Two stages of hydration swelling.Surface hydration expansion be formed in the mono layer adsorption that crystal layer surface occurs by hydrone, and
Clay particle or crystal layer are gradually pushed open, the chief motivation of this process is the adsorption energy of surface of clay aquation.Then may be used also
Second layer Water Molecular Adsorption etc. occurs, crystal layer spacing is gradually increased.After 4 layers of hydrone of brilliant Inter layer adsorption, surface hydration energy
No longer work.At this moment it due to much higher in the ion concentration dielectric phase between crystal layer, is mutually permeated so hydrone has from body
Into the trend of interlayer, this effect is known as osmotic effect, and generated penetration can be such that clay particle or crystal layer further divides
It opens.This stage is known as permeating the hydration swelling stage.The two expansion processes are influenced by the pH of solution, when solution is in acid
Property environment, expansion are suppressed, and when solution is in alkaline environment, montmorillonite can abundant water swelling.
Summary of the invention:
The present invention is provided a kind of blocked up using microorganism and the compound deep of clay gel in view of the above shortcomings of the prior art and adjusted
Method.The present invention carries out producing first the screening of alkali microorganism；Secondly, carrying out producing alkali microorganism and clay gel note for test oil reservoir
Enter the optimization of technique；Finally, carrying out the evaluation of field test and field test results.The present invention is by clay gel and produces the micro- life of alkali
The combination of object increases the pH value of water flooding using the ammonia that alkali microorganism generates in oil reservoir is produced, and makes to migrate to oil reservoir depth
The low concentration low-intensity clay gel in portion expands, and realizes that tune effect is blocked up in the deep of low concentration low-intensity clay gel, to expand
Water drive or subsequent other technology of reservoir sweep are related to volume, are finally reached the purpose of decreasing water cut and increasing oil.
Technique is adjusted using microorganism and the compound deep of clay gel are stifled the invention discloses a kind of, which is characterized in that the technique
Specifically includes the following steps:
(1) screening of oil reservoir is tested
The screening of oil reservoir is tested, specific screening criteria is as follows: reservoir temperature<80 DEG C, permeability>1000 × 10-3μm2。
(2) screening of alkali microorganism is produced
Produce alkali microorganism screening, the specific method is as follows: to 100ml test oil reservoir water flooding be added produce alkali microorganism and
The total 10ml of its nutrients, measures the pH value of solution；Solution is measured again after cultivating 15~20d under the conditions of testing reservoir temperature
PH value；It filters out pH value and changes maximum production alkali microorganism.
The production alkali microorganism is Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose aoxidizes Bacillus alcaligenes and production alkali is false single
One of born of the same parents bacterium.
The nutrients of the production alkali microorganism is 2~5g/L of starch, 0.5~1.0g/L of Dried Corn Steep Liquor Powder, phosphoric acid hydrogen two
0.1~0.3g/L of ammonium.
(3) it determines and produces alkali microbial fermentation solution and clay gel ratio
The determination method for producing alkali microorganism and clay gel ratio is as follows: loading back-up sand rock identical with test Reservoir Permeability
The heart；The water flooding of saturation testing oil reservoir is vacuumized, pore volume and measurement rock core water phase permeability k are calculated10；Prepare different proportion
Production alkali microbial fermentation solution and clay sol solution, stir evenly, formed mixed solution；Inject the above-mentioned different proportion of 0.01PV
Mixed solution cultivates 15~30d after the completion of injection；The water phase permeability k of measurement injection different proportion mixed solution rock core11；Meter
The fall of core permeability is calculated, the ratio of the corresponding mixed solution of permeability decrease amplitude maximum rock core is to determine to produce alkali
Microbial fermentation solution and clay gel ratio.
(4) total injection for producing alkali microbial fermentation solution and clay gel is determined
The method that the total injection of production alkali microbial fermentation solution and clay gel determines is as follows: loading and test Reservoir Permeability
Identical back-up sand rock core；The water flooding of saturation testing oil reservoir is vacuumized, pore volume and measurement rock core water phase permeability k are calculated20；
Preparation steps (3) determine the production alkali microbial fermentation solution and clay sol solution of ratio, stir evenly, and form mixed solution；Injection
Different amounts of mixed solution cultivates 15~30d after the completion of injection；Measure the water phase permeability of different injection rate mixed solution rock cores
k21；The fall of core permeability is calculated, the injection rate of the corresponding mixed solution of permeability decrease amplitude maximum rock core is chosen
As produce the total injection of alkali microbial fermentation solution and clay gel.
Produce the alkali microbial fermentation solution and the total injection of clay gel is 0.01~0.05PV.
(5) preparation of mixed solution
The specific method is as follows for the preparation of mixed solution: suspending agent is added into the water flooding of test oil reservoir, is configured to matter
Amount concentration is 0.2~0.5% suspension solution；Secondly, the clay gel of aforementioned proportion is added into above-mentioned solution, stirred when being added
It mixes, mixing speed is 200~300rpm, and mixing time is 30~60min；It is added and produces according to aforementioned proportion after mixing time
Alkali microbial fermentation solution, is uniformly mixing to obtain mixed solution.
The clay gel mass concentration be 1~3%, 3~7 μm of clay gel partial size；The suspending agent is biological polyoses
Or polyacrylamide.
(6) field test
Prepared mixed solution is injected from the water injection well of test oil reservoir using high-pressure plunger pump, injection rate 3
~5m3/h；According to 20~30% water fillings of former daily water-injection rate after the completion of mixed solution injection；Become a full member after 7d normal water filling.
The present invention generates ammonia using growth metabolism of the alkali microorganism in oil reservoir is produced, to keep the pH of the water flooding of oil reservoir slow
Slow to increase, clay gel starts to expand under alkaline environment, and mobility decline blocks up and intensity is adjusted to increase, can be achieved with oil reservoir in this way
Deep is stifled to adjust.Simultaneously as producing alkali microorganism growth metabolism needs the regular hour, thereby it is ensured that producing alkali microorganism and clay
Glue just expands after being able to enter oil deposit deep part, adjusts to realize that oil deposit deep part is stifled.
The present invention is had the following advantages and beneficial effects: compared with prior art
(1) production alkali microorganism of the invention and clay gel have cheap, while injection rate is lacked, and concentration is low, therefore,
The present invention has the advantages that cost of investment is low；
(2) microorganism that the present invention injects makes to glue in the pH value that underground role is not the displacement of reservoir oil but improves water flooding
Native glue expansion is realized that the deep of oil reservoir is stifled and is adjusted；
(3) phase modulation ratio is blocked up with conventional clay gel, the clay gum concentration that the present invention injects is low, and the intensity for blocking up tune is high, simultaneously
It is able to achieve the stifled tune in deep；
(4) clay gel expansion rate of the invention is high, and expansion rate can be improved 30% or more；
(5) present invention have simple process, the obvious feature of decreasing water cut and increasing oil effect, oil well comprehensive water cut decline 10% with
On, amount of increase in production is greater than 50%.
Specific embodiment
The following further describes the technical solution of the present invention combined with specific embodiments below.
Embodiment 1
Shengli Oil Field block G12For medium to high permeable water-drive pool, heterogeneity is very serious, and permeability is very poor to be reached
1.25,40 DEG C of reservoir temperature, permeability 2500 × 10-3μm2, pore volume 1.2 × 105m3, daily water-injection rate 120m3/ d, test
Preceding block aqueous 96.7%, block day oil-producing 64t/d.Implement the stifled tune in deep in the block using method of the invention, it is specific to walk
It is rapid as follows:
(1) screening of oil reservoir is tested
Test block G12Reservoir temperature<80 DEG C, permeability>1000 × 10-3μm2, therefore, meet oil reservoir sieve of the invention
Select standard.
(2) screening of alkali microorganism is produced
Block G is tested to 100ml12Water flooding be added and produce alkali microorganism, respectively Bacillus foecalis alkaligenes, alcaligenes eutrophus
Bacillus alcaligenes is aoxidized with xylose, while it is total that nutrients starch 2g/L, Dried Corn Steep Liquor Powder 0.5g/L, diammonium hydrogen phosphate 0.1g/L is added
10ml, the pH value for measuring solution is respectively 7,7 and 6.9；After cultivating 15d under the conditions of 40 DEG C of temperature, the pH of solution is measured again
Value is respectively 9,10.5 and 11；Filtering out the maximum production alkali microorganism that pH value changes under the test reservoir condition is xylose oxygen
Change Bacillus alcaligenes.
(3) xylose oxidation Bacillus alcaligenes fermentation liquid and clay gel ratio are determined
Firstly, loading permeability is 2500 × 10-3μm2Back-up sand rock core；Vacuumize saturation testing block G12Water flooding,
Calculate pore volume (PV) and measurement rock core water phase permeability k10(being shown in Table 1)；The xylose for preparing different proportion aoxidizes Bacillus alcaligenes
Fermentation liquid and clay sol solution (being shown in Table 1), stir evenly, and form mixed solution；Inject the mixing of the above-mentioned different proportion of 0.01PV
Solution cultivates 15d after the completion of injection；The water phase permeability k of measurement injection different proportion mixed solution rock core11(being shown in Table 1)；Pass through
Calculate core permeability fall obtain xylose oxidation Bacillus alcaligenes fermentation liquid and clay gel ratio be 1:3 when, rock core infiltration
Saturating rate fall highest is 92.08%.
1 different proportion xylose of table aoxidizes Bacillus alcaligenes fermentation liquid and clay gel core data list
(4) total injection of xylose oxidation Bacillus alcaligenes fermentation liquid and clay gel is determined
Loading permeability is 2500 × 10-3μm2Back-up sand rock core；Vacuumize saturation testing block G12Water flooding, calculate hole
Gap volume and measurement rock core water phase permeability k20, it is shown in Table 2；Xylose aoxidizes Bacillus alcaligenes fermentation liquid and clay sol solution according to 1:3
Ratio mixing after, stir evenly, formed mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV) cultivates 15d after the completion of injection；Measure the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 2；The fall of core permeability is calculated, permeability decrease amplitude maximum rock core is injection rate 0.03PV, therefore, corresponding
Mixed solution injection rate be 0.03PV it is best.
2 different proportion xylose of table aoxidizes Bacillus alcaligenes fermentation liquid and clay gel core data list
(5) preparation of mixed solution
To test block G12Water flooding in be added biological polyoses be used as suspending agent, be configured to mass concentration be 0.5% hang
Floating agent solution；Secondly, clay gel is added into above-mentioned solution, 5 μm of average grain diameter, stir while adding, mixing speed is
200rpm, mixing time 60min, clay gel mass concentration are 2.5%；The xylose of aforementioned proportion is added after mixing time
Bacillus alcaligenes fermentation liquid is aoxidized, mixed solution is uniformly mixing to obtain.
(6) field test
Prepared mixed solution is injected from the water injection well of test block using high-pressure plunger pump, injection rate is
3m3/ h, injection rate 0.03PV are 3.6 × 103m3；It is filled the water after the completion of mixed solution injection according to the 20% of former daily water-injection rate,
24m3/d；Then become a full member normal water filling.Test block G12It is aqueous to drop to 83.4% by testing preceding 96.7%, have dropped 13.4 hundred
Branch, block day oil-producing from 64t/d rise to 120t/d, amount of increase in production has reached 97.5%, and field test results are good.
Embodiment 2
Shengli Oil Field block G15For medium to high permeable water-drive pool, heterogeneity is very serious, and permeability is very poor to be reached
1.32,60 DEG C of reservoir temperature, permeability 2100 × 10-3μm2, pore volume 1.5 × 105m3, daily water-injection rate 100m3/ d, test
Preceding block aqueous 97.3%, block day oil-producing 32t/d.Implement the stifled tune in deep in the block using method of the invention, it is specific to walk
It is rapid as follows:
(1) screening of oil reservoir is tested
Test block G15Reservoir temperature<80 DEG C, permeability>1000 × 10-3μm2, therefore, meet oil reservoir sieve of the invention
Select standard.
(2) screening of alkali microorganism is produced
To 100ml test block water flooding be added produce alkali microorganism alcaligenes eutrophus, xylose oxidation Bacillus alcaligenes and
Pseudomonas alcaligenes, while nutrients starch 5g/L, Dried Corn Steep Liquor Powder 0.7g/L, the total 10ml of diammonium hydrogen phosphate 0.2g/L is added,
The pH value for measuring solution is respectively 6.9,6.8 and 7；In test block G15After cultivating 18d under the conditions of 60 DEG C of reservoir temperature, survey again
The pH value for determining solution is respectively 10.5,11 and 12；It is micro- to filter out the maximum production alkali that pH value changes under the conditions of the test block
Biology is Pseudomonas alcaligenes.
(3) Pseudomonas alcaligenes fermentation liquid and clay gel ratio are determined
Firstly, loading permeability is 2100 × 10-3μm2Back-up sand rock core；The water flooding of saturation testing block is vacuumized, is counted
Calculate pore volume PV and measurement rock core water phase permeability k10(being shown in Table 3)；Third prepares the Pseudomonas alcaligenes fermentation of different proportion
Liquid and clay sol solution (being shown in Table 3), stir evenly, and form mixed solution；The mixed solution of the above-mentioned different proportion of 0.01PV is injected,
20d is cultivated after the completion of injection；The water phase permeability k of measurement injection different proportion mixed solution rock core11(being shown in Table 3)；Pass through calculating
The fall of core permeability obtains Pseudomonas alcaligenes fermentation liquid and clay gel ratio when being 1:4, core permeability decline
Amplitude reaches highest, rate of descent 94.5%.
3 different proportion Pseudomonas alcaligenes fermentation liquid of table and clay gel core data list
(4) total injection of Pseudomonas alcaligenes fermentation liquid and clay gel is determined
Loading permeability is 2100 × 10-3μm2Back-up sand rock core；Vacuumize saturation testing block G15Water flooding, calculate
Pore volume and measurement rock core water phase permeability k20, it is shown in Table 4；Pseudomonas alcaligenes fermentation liquid and clay sol solution are according to 1:4's
It after ratio mixing, stirs evenly, forms mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV) cultivates 20d after the completion of injection；Measure the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 4；The fall of core permeability is calculated, permeability decrease amplitude is up to the corresponding injection rate of 95.3% rock core
0.04PV, therefore, the total injection of Pseudomonas alcaligenes fermentation liquid and clay gel are 0.04PV.
4 different proportion Pseudomonas alcaligenes fermentation liquid of table and clay gel core data list
(5) preparation of mixed solution
To test block G15Water flooding in be added polyacrylamide be used as suspending agent, be configured to mass concentration be 0.2%
Suspension solution；Secondly, clay gel is added into above-mentioned solution, 7 μm of average grain diameter, stir while adding, mixing speed is
250rpm, mixing time 30min, clay gel mass concentration are 1.0%；The production alkali of aforementioned proportion is false single after mixing time
Born of the same parents' fermented liquid, obtains mixed solution.
(6) field test
Prepared mixed solution is injected from the water injection well of test block using high-pressure plunger pump, injection rate is
4m3/ h, injection rate 0.04PV are 6.0 × 103m3；It is filled the water after the completion of mixed solution injection according to the 30% of former daily water-injection rate,
30m3/d；Then become a full member normal water filling.Test block G15It is aqueous to drop to 80.2% by testing preceding 97.3%, have dropped 17.1 hundred
Branch, block day oil-producing from 32t/d rise to 78t/d, amount of increase in production has reached 144%, and field test results are good.
Embodiment 3
Shengli Oil Field block G32For Thief zone water-drive pool, heterogeneity is very serious, and permeability is very poor to reach 1.50,
75 DEG C of reservoir temperature, permeability 3200 × 10-3μm2, pore volume 7.0 × 105m3, daily water-injection rate 200m3/ d tests proparea
Block aqueous 98.0%, block day oil-producing 43t/d.Implement the stifled tune in deep in the block using method of the invention, specific steps are such as
Under:
(1) screening of oil reservoir is tested
Test block G32Reservoir temperature<80 DEG C, permeability>1000 × 10-3μm2, therefore, meet oil reservoir sieve of the invention
Select standard.
(1) screening of alkali microorganism is produced
Block G is tested to 100ml32Water flooding be added produce alkali microorganism Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose oxygen
Change Bacillus alcaligenes and Pseudomonas alcaligenes, while nutrients starch 3g/L, Dried Corn Steep Liquor Powder 1.0g/L, diammonium hydrogen phosphate is added
The total 10ml of 0.3g/L, the pH value for measuring solution is respectively 7.0,7.1,6.9 and 7.0；In test block G3275 DEG C of items of reservoir temperature
After cultivating 20d under part, the pH value for measuring solution again is respectively as follows: 9.5,11,10.5 and 9；It filters out in the test reservoir condition
It is alcaligenes eutrophus that lower pH value, which changes maximum production alkali microorganism,.
(3) alcaligenes eutrophus fermentation liquid and clay gel ratio are determined
Firstly, loading permeability is 3200 × 10-3μm2Back-up sand rock core；Vacuumize saturation testing block G32Water flooding, meter
Calculate pore volume and measurement rock core water phase permeability k10, it is shown in Table 5；Prepare the alcaligenes eutrophus fermentation liquid and clay of different proportion
Sol solution (is shown in Table 5), stirs evenly, and forms mixed solution；The mixed solution of the above-mentioned different proportion of 0.01PV is injected, injection is completed
After cultivate 30d；The water phase permeability k of measurement injection different proportion mixed solution rock core11, it is shown in Table 5；Finally, by calculating rock core
The fall of permeability obtains alcaligenes eutrophus fermentation liquid and clay gel ratio when being 1:4, core permeability fall
Reach highest, rate of descent 94.60%.
5 different proportion alcaligenes eutrophus fermentation liquid of table and clay gel core data list
(4) total injection of alcaligenes eutrophus fermentation liquid and clay gel is determined
Firstly, loading permeability is 3200 × 10-3μm2Back-up sand rock core；Vacuumize saturation testing block G32Water flooding, meter
Calculate pore volume and measurement rock core water phase permeability k20, it is shown in Table 6；Alcaligenes eutrophus fermentation liquid and clay sol solution are according to 1:4
Ratio mixing after, stir evenly, formed mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV) cultivates 30d after the completion of injection；Measure the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 6；Finally, calculating the fall of core permeability, permeability decrease amplitude maximum rock core is injection rate 0.03PV, because
This, the total injection of alcaligenes eutrophus fermentation liquid and clay gel is 0.03PV.
6 different proportion alcaligenes eutrophus fermentation liquid of table and clay gel core data list
(5) preparation of mixed solution
To test block G32Water flooding in be added biological polyoses be used as suspending agent, be configured to mass concentration be 0.3% hang
Floating agent solution；Secondly, clay gel is added into above-mentioned solution, 3 μm of average grain diameter, stir while adding, mixing speed is
300rpm, mixing time 50min, clay gel mass concentration are 3.0%；Really supporting for aforementioned proportion produces alkali after mixing time
Bacillus fermentation liquid, obtains mixed solution.
(6) field test
Prepared mixed solution is injected from the water injection well of test block using high-pressure plunger pump, injection rate is
5m3/ h, injection rate 0.03PV are 2.1 × 103m3；It is filled the water after the completion of mixed solution injection according to the 25% of former daily water-injection rate,
75m3/d；Then become a full member normal water filling.Test block G32It is aqueous to drop to 83.5% by testing preceding 98.0%, have dropped 14.5 hundred
Branch, block day oil-producing from 43t/d rise to 95t/d, amount of increase in production has reached 120.9%, and field test results are good.
Claims (9)
1. a kind of adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that the technique specifically includes following step
It is rapid:
(1) screening of oil reservoir is tested；
(2) screening of alkali microorganism is produced；
(3) it determines and produces alkali microbial fermentation solution and clay gel ratio；
(4) total injection for producing alkali microbial fermentation solution and clay gel is determined；
(5) preparation of mixed solution；
(6) field test；
The screening of the production alkali microorganism, the specific method is as follows: being added to the water flooding of 100ml test oil reservoir and produces alkali microorganism
And its total 10ml of nutrients, measure the pH value of solution；Solution is measured again after cultivating 15~20d under the conditions of testing reservoir temperature
PH value；Filter out the maximum production alkali microorganism of pH value change.
2. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
The screening of oil reservoir is tested, specific screening criteria is as follows: reservoir temperature<80 DEG C, permeability>1000 × 10-3μm2。
3. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
Production alkali microorganism is Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose oxidation one of Bacillus alcaligenes and Pseudomonas alcaligenes；Institute
State production alkali microorganism nutrients be 2~5g/L of starch, 0.5~1.0g/L of Dried Corn Steep Liquor Powder, diammonium hydrogen phosphate 0.1~
0.3g/L。
4. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
The determination method for producing alkali microorganism and clay gel ratio is as follows: loading back-up sand rock core identical with test Reservoir Permeability；It takes out true
The water flooding of empty saturation testing oil reservoir calculates pore volume and measurement rock core water phase permeability k10；Prepare the production alkali of different proportion
Microbial fermentation solution and clay sol solution, stir evenly, and form mixed solution；The mixing for injecting the above-mentioned different proportion of 0.01PV is molten
Liquid cultivates 15~30d after the completion of injection；The water phase permeability k of measurement injection different proportion mixed solution rock core11；Calculate rock core
The fall of permeability, the ratio of the corresponding mixed solution of permeability decrease amplitude maximum rock core are to determine to produce alkali microorganism
Fermentation liquid and clay gel ratio.
5. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
It is as follows to produce the method that the total injection of alkali microbial fermentation solution and clay gel determines: loading and tests that Reservoir Permeability is identical to be filled out
The sandstone heart；The water flooding of saturation testing oil reservoir is vacuumized, pore volume and measurement rock core water phase permeability k are calculated20；It prepares above-mentioned
The production alkali microbial fermentation solution and clay sol solution of ratio, stir evenly, and form mixed solution；It is molten to inject different amounts of mixing
Liquid cultivates 15~30d after the completion of injection；Measure the water phase permeability k of different injection rate mixed solution rock cores21；Rock core is calculated to seep
The fall of saturating rate, the injection rate for choosing the corresponding mixed solution of permeability decrease amplitude maximum rock core are to produce alkali microorganism
The total injection of fermentation liquid and clay gel.
6. according to claim 5 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
The total injection for producing alkali microbial fermentation solution and clay gel is 0.01~0.05PV.
7. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
The preparation of mixed solution, the specific method is as follows: suspending agent is added into the water flooding of test oil reservoir, being configured to mass concentration is
0.2~0.5% suspension solution；Secondly, clay gel is added into above-mentioned solution, stir while adding, mixing speed be 200~
300rpm, mixing time are 30~60min；It is added after mixing time according to aforementioned proportion and produces alkali microbial fermentation solution, stirred
It mixes and uniformly obtains mixed solution.
8. according to claim 7 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
Suspending agent is biological polyoses or polyacrylamide.
9. according to claim 1 adjust technique using microorganism and the compound deep of clay gel are stifled, which is characterized in that described
Field test, the specific steps are as follows: infuse prepared mixed solution from the water injection well of test block using high-pressure plunger pump
Enter, injection rate is 3~5m3/h；According to 20~30% water fillings of former daily water-injection rate after the completion of mixed solution injection；Become a full member after 7d
Often water filling.
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148303A (en) * | 2006-09-24 | 2008-03-26 | 江苏博大环保股份有限公司 | Oil field re-injection water microbiological treatment |
| CN103114833A (en) * | 2011-11-17 | 2013-05-22 | 中国石油化工股份有限公司 | Microbe oil extraction method for activating oil deposit deep portion function flora |
| CN103147731A (en) * | 2013-03-25 | 2013-06-12 | 北京大学 | Method for enhancing crude oil recovery ratio |
| CN103291267A (en) * | 2013-06-14 | 2013-09-11 | 中国石油化工股份有限公司 | Method for improving oil well yields by means of oil pool indigenous microorganisms |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN105201471A (en) * | 2015-09-28 | 2015-12-30 | 中国石油化工股份有限公司 | Microbial enhanced oil recovery method |
| CN105626014A (en) * | 2015-07-29 | 2016-06-01 | 中国石油化工股份有限公司 | Method for improving oil well production through microorganism single well processing |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| MX336364B (en) * | 2009-12-21 | 2015-11-06 | Inst Mexicano Del Petróleo | Biotechnological process for hydrocarbon recovery in low permeability porous media. |
-
2017
- 2017-08-25 CN CN201710740174.5A patent/CN107476778B/en active Active
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148303A (en) * | 2006-09-24 | 2008-03-26 | 江苏博大环保股份有限公司 | Oil field re-injection water microbiological treatment |
| CN103114833A (en) * | 2011-11-17 | 2013-05-22 | 中国石油化工股份有限公司 | Microbe oil extraction method for activating oil deposit deep portion function flora |
| CN103147731A (en) * | 2013-03-25 | 2013-06-12 | 北京大学 | Method for enhancing crude oil recovery ratio |
| CN103291267A (en) * | 2013-06-14 | 2013-09-11 | 中国石油化工股份有限公司 | Method for improving oil well yields by means of oil pool indigenous microorganisms |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN105626014A (en) * | 2015-07-29 | 2016-06-01 | 中国石油化工股份有限公司 | Method for improving oil well production through microorganism single well processing |
| CN105201471A (en) * | 2015-09-28 | 2015-12-30 | 中国石油化工股份有限公司 | Microbial enhanced oil recovery method |
Non-Patent Citations (1)
| Title |
|---|
| 微生物堵调研究进展;汪卫东等;《油气地质与采收率》;20070125;第14卷(第1期);第88-89页 |
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