CN107476778A - One kind adjusts technique using microorganism and the compound deep of clay gel are stifled - Google Patents
One kind adjusts technique using microorganism and the compound deep of clay gel are stifled Download PDFInfo
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- CN107476778A CN107476778A CN201710740174.5A CN201710740174A CN107476778A CN 107476778 A CN107476778 A CN 107476778A CN 201710740174 A CN201710740174 A CN 201710740174A CN 107476778 A CN107476778 A CN 107476778A
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- 239000004927 clay Substances 0.000 title claims abstract description 85
- 244000005700 microbiome Species 0.000 title claims abstract description 49
- 238000000034 method Methods 0.000 title claims abstract description 32
- 150000001875 compounds Chemical class 0.000 title claims abstract description 16
- 238000002347 injection Methods 0.000 claims abstract description 75
- 239000007924 injection Substances 0.000 claims abstract description 75
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 71
- 239000011259 mixed solution Substances 0.000 claims abstract description 55
- 239000003513 alkali Substances 0.000 claims abstract description 54
- 239000000243 solution Substances 0.000 claims abstract description 52
- 238000004519 manufacturing process Methods 0.000 claims abstract description 41
- 238000012360 testing method Methods 0.000 claims abstract description 35
- 238000000855 fermentation Methods 0.000 claims abstract description 20
- 230000004151 fermentation Effects 0.000 claims abstract description 20
- 238000012216 screening Methods 0.000 claims abstract description 19
- 230000000813 microbial effect Effects 0.000 claims abstract description 18
- 238000002360 preparation method Methods 0.000 claims abstract description 9
- 230000035699 permeability Effects 0.000 claims description 53
- 239000011435 rock Substances 0.000 claims description 40
- 238000002474 experimental method Methods 0.000 claims description 27
- 239000012530 fluid Substances 0.000 claims description 25
- SRBFZHDQGSBBOR-IOVATXLUSA-N D-xylopyranose Chemical compound O[C@@H]1COC(O)[C@H](O)[C@H]1O SRBFZHDQGSBBOR-IOVATXLUSA-N 0.000 claims description 24
- 238000002156 mixing Methods 0.000 claims description 23
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 18
- 241000588986 Alcaligenes Species 0.000 claims description 14
- 241000252867 Cupriavidus metallidurans Species 0.000 claims description 14
- 241000168225 Pseudomonas alcaligenes Species 0.000 claims description 12
- PYMYPHUHKUWMLA-UHFFFAOYSA-N arabinose Natural products OCC(O)C(O)C(O)C=O PYMYPHUHKUWMLA-UHFFFAOYSA-N 0.000 claims description 12
- SRBFZHDQGSBBOR-UHFFFAOYSA-N beta-D-Pyranose-Lyxose Natural products OC1COC(O)C(O)C1O SRBFZHDQGSBBOR-UHFFFAOYSA-N 0.000 claims description 12
- 239000011148 porous material Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 12
- 239000004576 sand Substances 0.000 claims description 9
- 230000007423 decrease Effects 0.000 claims description 8
- 238000011049 filling Methods 0.000 claims description 8
- 239000000375 suspending agent Substances 0.000 claims description 7
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 230000003647 oxidation Effects 0.000 claims description 6
- 238000007254 oxidation reaction Methods 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 240000008042 Zea mays Species 0.000 claims description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
- 230000008859 change Effects 0.000 claims description 5
- 235000005822 corn Nutrition 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 claims description 4
- 229910000388 diammonium phosphate Inorganic materials 0.000 claims description 4
- 235000019838 diammonium phosphate Nutrition 0.000 claims description 4
- 229920002401 polyacrylamide Polymers 0.000 claims description 3
- 239000000725 suspension Substances 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 2
- 238000009738 saturating Methods 0.000 claims description 2
- 238000011161 development Methods 0.000 abstract description 4
- 230000003247 decreasing effect Effects 0.000 abstract description 3
- 239000003129 oil well Substances 0.000 abstract description 2
- 230000008092 positive effect Effects 0.000 abstract description 2
- 239000003921 oil Substances 0.000 description 42
- 239000010410 layer Substances 0.000 description 7
- 239000002981 blocking agent Substances 0.000 description 5
- 239000013078 crystal Substances 0.000 description 5
- 230000036571 hydration Effects 0.000 description 5
- 238000006703 hydration reaction Methods 0.000 description 5
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 4
- 206010042674 Swelling Diseases 0.000 description 4
- GUJOJGAPFQRJSV-UHFFFAOYSA-N dialuminum;dioxosilane;oxygen(2-);hydrate Chemical group O.[O-2].[O-2].[O-2].[Al+3].[Al+3].O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O GUJOJGAPFQRJSV-UHFFFAOYSA-N 0.000 description 4
- 238000006073 displacement reaction Methods 0.000 description 4
- 239000003292 glue Substances 0.000 description 4
- 229910052901 montmorillonite Inorganic materials 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 230000008961 swelling Effects 0.000 description 4
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000009671 shengli Substances 0.000 description 3
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 description 2
- ZGINPHARJIMXMG-UJPDDDSFSA-N [O].OC[C@@H](O)[C@H](O)[C@@H](O)C=O Chemical group [O].OC[C@@H](O)[C@H](O)[C@@H](O)C=O ZGINPHARJIMXMG-UJPDDDSFSA-N 0.000 description 2
- 229910021529 ammonia Inorganic materials 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000007667 floating Methods 0.000 description 2
- 239000011229 interlayer Substances 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000324499 Alkalibacillus Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229910000147 aluminium phosphate Inorganic materials 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010779 crude oil Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 239000007863 gel particle Substances 0.000 description 1
- 239000001257 hydrogen Substances 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000008450 motivation Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000037361 pathway Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
Classifications
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B33/00—Sealing or packing boreholes or wells
- E21B33/10—Sealing or packing boreholes or wells in the borehole
- E21B33/13—Methods or devices for cementing, for plugging holes, crevices or the like
-
- E—FIXED CONSTRUCTIONS
- E21—EARTH OR ROCK DRILLING; MINING
- E21B—EARTH OR ROCK DRILLING; OBTAINING OIL, GAS, WATER, SOLUBLE OR MELTABLE MATERIALS OR A SLURRY OF MINERALS FROM WELLS
- E21B43/00—Methods or apparatus for obtaining oil, gas, water, soluble or meltable materials or a slurry of minerals from wells
- E21B43/16—Enhanced recovery methods for obtaining hydrocarbons
- E21B43/20—Displacing by water
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- Geology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Mining & Mineral Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Fluid Mechanics (AREA)
- Physics & Mathematics (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Geochemistry & Mineralogy (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Cosmetics (AREA)
Abstract
The invention belongs to oil-gas field development technical field, and in particular to one kind adjusts technique using microorganism and the compound deep of clay gel are stifled, and described technique specifically includes following steps：Test the screening of oil reservoir；Produce the screening of alkali microorganism；It is determined that production alkali microbial fermentation solution and clay gel ratio；It is determined that the total injection of production alkali microbial fermentation solution and clay gel；The preparation of mixed solution；Field test.The present invention has technique simple, blocks up that the intensity adjusted is high, and cost of investment is low, and the characteristics of decreasing water cut and increasing oil positive effect, oil well comprehensive water cut declines more than 10%, and amount of increase in production is more than 50%.Therefore, present invention is generally applicable in the stifled tune technique in the deep of medium to high permeable oil reservoir.
Description
Technical field
The invention belongs to oil-gas field development technical field, and in particular to one kind is blocked up using microorganism and the compound deep of clay gel
Adjust technique.
Background technology
Medium to high permeable oil reservoir easily forms current predominant pathway (macropore) during long-period water drive is developed, due to oil
The anisotropism of Tibetan is serious, reduces the swept volume of waterflooding development, so as to have impact on the development effectiveness of water drive.Therefore, it is necessary to
Water plugging and profile controlling processing is carried out, to improve the swept volume of water drive or other displacement technologies, so as to further improve the crude oil of oil reservoir
Recovery ratio.
The blocking agent for being presently used for stifled tune is faced with " injection efficiency " contradiction taken into account different from " intensity ", if blocking agent has
There is preferable intensity, then its mobility is with regard to undesirable, it is difficult to oil deposit deep part is injected, if on the contrary, blocking agent has preferably stream
Dynamic property, then its intensity may be inadequate, it is difficult to play preferably stifled tune effect.Therefore, to solve it is this " note do not enter, it is stifled not
Contradiction firmly " can be injected into oil deposit deep part, it is necessary to blocking agent has preferable mobility in injection, and when blocking agent, to reach oil reservoir deep
It behind portion, can voluntarily change, make its mobility variations poor, and the intensity blocked becomes big, realizes that deep is stifled and adjusts.
Clay gel main component is montmorillonite, due to cheap and be readily available, once for the displacement of reservoir oil, but when concentration is too high
(being higher than 5%), although there is enough stifled tune intensity, it is difficult to inject, (is less than 3%) when concentration is too low, although easily
Injection, but stifled tune insufficient strength.Concentration has certain oil displacement efficiency in the range of 3%~5%, but still has substantial amounts of
Clay gel is from producing well output, it is impossible to realizes that " deep " truly is stifled and adjusts.
Clay glue host is montmorillonite, montmorillonite water swelling, and its mechanism of intumescence can be divided into surface hydration expansion and infiltration
Two stages of hydration swelling.Surface hydration expansion is what is formed by hydrone in the mono layer adsorption that crystal layer surface occurs, and
Clay particle or crystal layer are gradually pushed open, the chief motivation of this process is the energy of adsorption of surface of clay aquation.Then may be used also
Generation second layer Water Molecular Adsorption etc., crystal layer spacing gradually increases.After 4 layers of hydrone of brilliant Inter layer adsorption, surface hydration energy
No longer work.At this moment due to much higher in the ion concentration medium body phase between crystal layer, permeated so hydrone has from body phase
Into the trend of interlayer, this effect is referred to as osmotic effect, and caused penetration can be such that clay particle or crystal layer further divides
Open.This stage is referred to as permeating the hydration swelling stage.The two expansion processes are influenceed by the pH of solution, when solution is in acid
Property environment, its expansion are suppressed, and when solution is in alkaline environment, montmorillonite can abundant water swelling.
The content of the invention：
The present invention is provided a kind of blocked up using microorganism and the compound deep of clay gel for above-mentioned the deficiencies in the prior art and adjusted
Method.The present invention carries out producing first the screening of alkali microorganism；Secondly, carry out producing alkali microorganism for experiment oil reservoir and clay gel is noted
Enter the optimization of technique；Finally, the evaluation of field test and field test results is carried out.The present invention is by clay gel and produces the micro- life of alkali
The combination of thing, the pH value of stratum water is raised using alkali microorganism caused ammonia in oil reservoir is produced, make to migrate to oil reservoir depth
The low concentration low-intensity clay gel in portion expands, and realizes that tune effect is blocked up in the deep of low concentration low-intensity clay gel, so as to expand
The purpose for being related to volume, being finally reached decreasing water cut and increasing oil of water drive or follow-up other technology of reservoir sweep.
Technique is adjusted using microorganism and the compound deep of clay gel are stifled, it is characterised in that the technique the invention discloses one kind
Specifically include following steps：
(1) screening of oil reservoir is tested
The screening of oil reservoir is tested, specific screening criteria is as follows：Reservoir temperature<80 DEG C, permeability>1000×10-3μm2。
(2) screening of alkali microorganism is produced
The screening of alkali microorganism is produced, specific method is as follows：To the stratum water of 100ml experiment oil reservoirs add production alkali microorganism and
The common 10ml of its nutrients, determine the pH value of solution；Solution is determined again after 15~20d is cultivated under the conditions of testing reservoir temperature
PH value；Filter out pH value and change maximum production alkali microorganism.
Described production alkali microorganism is Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose aoxidizes Bacillus alcaligenes and production alkali is false single
One kind in born of the same parents bacterium.
The nutrients of described production alkali microorganism is 2~5g/L of starch, 0.5~1.0g/L of Dried Corn Steep Liquor Powder, phosphoric acid hydrogen two
0.1~0.3g/L of ammonium.
(3) production alkali microbial fermentation solution and clay gel ratio are determined
The determination method for producing alkali microorganism and clay gel ratio is as follows：Load and experiment Reservoir Permeability identical back-up sand rock
The heart；The stratum water of saturation testing oil reservoir is vacuumized, calculates pore volume and measure rock core water phase permeability k10；Prepare different proportion
Production alkali microbial fermentation solution and clay sol solution, stir, formed mixed solution；Inject the above-mentioned different proportions of 0.01PV
Mixed solution, 15~30d is cultivated after the completion of injection；The water phase permeability k of measure injection different proportion mixed solution rock core11；Meter
The fall of core permeability is calculated, the ratio of mixed solution corresponding to permeability decrease amplitude maximum rock core is to determine production alkali
Microbial fermentation solution and clay gel ratio.
(4) total injection of production alkali microbial fermentation solution and clay gel is determined
The method for producing the total injection determination of alkali microbial fermentation solution and clay gel is as follows：Load and experiment Reservoir Permeability
Identical back-up sand rock core；The stratum water of saturation testing oil reservoir is vacuumized, calculates pore volume and measure rock core water phase permeability k20；
Preparation steps (3) determine the production alkali microbial fermentation solution and clay sol solution of ratio, stir, and form mixed solution；Injection
Different amounts of mixed solution, 15~30d is cultivated after the completion of injection；Determine the water phase permeability of different injection rate mixed solution rock cores
k21；The fall of core permeability is calculated, chooses the injection rate of mixed solution corresponding to permeability decrease amplitude maximum rock core
As produce the total injection of alkali microbial fermentation solution and clay gel.
Described production alkali microbial fermentation solution and the total injection of clay gel are 0.01~0.05PV.
(5) preparation of mixed solution
The specific method of the preparation of mixed solution is as follows：Suspending agent is added into the stratum water of experiment oil reservoir, is configured to matter
Amount concentration is 0.2~0.5% suspension solution；Secondly, the clay gel of aforementioned proportion is added into above-mentioned solution, is stirred when adding
Mix, mixing speed is 200~300rpm, and mixing time is 30~60min；Mixing time is added according to aforementioned proportion after terminating and produced
Alkali microbial fermentation solution, is uniformly mixing to obtain mixed solution.
Described clay gel mass concentration is 1~3%, 3~7 μm of clay gel particle diameter；Described suspending agent is biological polyoses
Or polyacrylamide.
(6) field test
High-pressure plunger pump is utilized to be injected from the water injection well of experiment oil reservoir the mixed solution prepared, injection rate 3
~5m3/h；According to 20~30% water fillings of former daily water-injection rate after the completion of mixed solution injection；Become a full member after 7d normal water filling.
The present invention produces ammonia using growth metabolism of the alkali microorganism in oil reservoir is produced, so that the pH of the stratum water of oil reservoir delays
Slow rise, clay gel start to expand under alkaline environment, and mobility declines, and block up and adjust intensity rise, so can be achieved with oil reservoir
Deep is stifled to adjust.Simultaneously as production alkali microorganism growth metabolism needs the regular hour, thereby it is ensured that production alkali microorganism and clay
Glue just expands after can entering oil deposit deep part, is adjusted so as to realize that oil deposit deep part is stifled.
The present invention has the following advantages that compared with prior art and beneficial effect：
(1) of the invention production alkali microorganism and clay gel have cheap, while injection rate is lacked, and concentration is low, therefore,
The present invention has the advantages of cost of investment is low；
(2) microorganism that the present invention injects makes to glue in the pH value that underground role is not the displacement of reservoir oil but improves stratum water
Native glue expansion, realize that the deep of oil reservoir is stifled and adjust；
(3) phase modulation ratio is blocked up with conventional clay gel, the clay gum concentration that the present invention injects is low, and the stifled intensity adjusted is high, simultaneously
It can realize that deep is stifled to adjust；
(4) clay gel expansion rate of the invention is high, and expansion rate can improve more than 30%；
(5) present invention has technique simple, the characteristics of decreasing water cut and increasing oil positive effect, oil well comprehensive water cut decline 10% with
On, amount of increase in production is more than 50%.
Embodiment
Technical scheme is described further with reference to specific embodiment.
Embodiment 1
Shengli Oil Field block G12For medium to high permeable water-drive pool, anisotropism is very serious, and permeability extreme difference reaches
1.25,40 DEG C of reservoir temperature, permeability 2500 × 10-3μm2, pore volume 1.2 × 105m3, daily water-injection rate 120m3/ d, experiment
Preceding block aqueous 96.7%, block day oil-producing 64t/d.Implement the stifled tune in deep, specific step in the block using the method for the present invention
It is rapid as follows：
(1) screening of oil reservoir is tested
Test block G12Reservoir temperature<80 DEG C, permeability>1000×10-3μm2, therefore, meet the oil reservoir sieve of the present invention
Select standard.
(2) screening of alkali microorganism is produced
To 100ml experiment blocks G12Stratum water add production alkali microorganism, respectively Bacillus foecalis alkaligenes, alcaligenes eutrophus
Bacillus alcaligenes is aoxidized with xylose, while adds nutrients starch 2g/L, Dried Corn Steep Liquor Powder 0.5g/L, diammonium hydrogen phosphate 0.1g/L and is total to
10ml, the pH value for determining solution are respectively 7,7 and 6.9；After cultivating 15d under 40 DEG C of temperature conditionss, the pH of solution is determined again
Value is respectively 9,10.5 and 11；It is xylose oxygen to filter out the maximum production alkali microorganism that pH value changes under the experiment reservoir condition
Change Bacillus alcaligenes.
(3) xylose oxidation Bacillus alcaligenes zymotic fluid and clay gel ratio are determined
First, it is 2500 × 10 to load permeability-3μm2Back-up sand rock core；Vacuumize saturation testing block G12Stratum water,
Calculate pore volume (PV) and measure rock core water phase permeability k10(being shown in Table 1)；Prepare the xylose oxidation Bacillus alcaligenes of different proportion
Zymotic fluid and clay sol solution (being shown in Table 1), stir, and form mixed solution；Inject the mixing of the above-mentioned different proportions of 0.01PV
Solution, 15d is cultivated after the completion of injection；The water phase permeability k of measure injection different proportion mixed solution rock core11(being shown in Table 1)；Pass through
It is 1 that the fall of calculating core permeability, which obtains xylose oxidation Bacillus alcaligenes zymotic fluid and clay gel ratio,：When 3, rock core oozes
Saturating rate fall highest, it is 92.08%.
The different proportion xylose of table 1 aoxidizes Bacillus alcaligenes zymotic fluid and clay gel core data list
(4) total injection of xylose oxidation Bacillus alcaligenes zymotic fluid and clay gel is determined
It is 2500 × 10 to load permeability-3μm2Back-up sand rock core；Vacuumize saturation testing block G12Stratum water, calculate hole
Gap volume and measure rock core water phase permeability k20, it is shown in Table 2；Xylose aoxidizes Bacillus alcaligenes zymotic fluid and clay sol solution according to 1：3
Ratio mixing after, stir, formed mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV), 15d is cultivated after the completion of injection；Determine the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 2；The fall of core permeability is calculated, permeability decrease amplitude maximum rock core is injection rate 0.03PV, therefore, corresponding
Mixed solution injection rate for 0.03PV it is optimal.
The different proportion xylose of table 2 aoxidizes Bacillus alcaligenes zymotic fluid and clay gel core data list
(5) preparation of mixed solution
To experiment block G12Stratum water in add biological polyoses be used as suspending agent, be configured to mass concentration for 0.5% hang
Floating agent solution；Secondly, clay gel is added into above-mentioned solution, 5 μm of its average grain diameter, is stirred while adding, mixing speed is
200rpm, mixing time 60min, clay gel mass concentration are 2.5%；Mixing time adds the xylose of aforementioned proportion after terminating
Bacillus alcaligenes zymotic fluid is aoxidized, is uniformly mixing to obtain mixed solution.
(6) field test
High-pressure plunger pump is utilized to be injected from the water injection well of experiment block the mixed solution prepared, injection rate is
3m3/ h, injection rate 0.03PV, it is 3.6 × 103m3；Mixed solution injection after the completion of according to former daily water-injection rate 20% water filling,
24m3/d；Then become a full member normal water filling.Test block G12It is aqueous to drop to 83.4% by testing preceding 96.7%, it have dropped 13.4 hundred
Branch, block day oil-producing from 64t/d rise to 120t/d, amount of increase in production has reached 97.5%, and field test results are good.
Embodiment 2
Shengli Oil Field block G15For medium to high permeable water-drive pool, anisotropism is very serious, and permeability extreme difference reaches
1.32,60 DEG C of reservoir temperature, permeability 2100 × 10-3μm2, pore volume 1.5 × 105m3, daily water-injection rate 100m3/ d, experiment
Preceding block aqueous 97.3%, block day oil-producing 32t/d.Implement the stifled tune in deep, specific step in the block using the method for the present invention
It is rapid as follows：
(1) screening of oil reservoir is tested
Test block G15Reservoir temperature<80 DEG C, permeability>1000×10-3μm2, therefore, meet the oil reservoir sieve of the present invention
Select standard.
(2) screening of alkali microorganism is produced
To the stratum water of 100ml experiment blocks add production alkali microorganism alcaligenes eutrophus, xylose oxidation Bacillus alcaligenes and
Pseudomonas alcaligenes, while nutrients starch 5g/L, Dried Corn Steep Liquor Powder 0.7g/L, the common 10ml of diammonium hydrogen phosphate 0.2g/L are added,
The pH value for determining solution is respectively 6.9,6.8 and 7；In experiment block G15After cultivating 18d under the conditions of 60 DEG C of reservoir temperature, survey again
The pH value for determining solution is respectively 10.5,11 and 12；It is micro- to filter out the maximum production alkali that pH value changes under the conditions of the experiment block
Biology is Pseudomonas alcaligenes.
(3) Pseudomonas alcaligenes zymotic fluid and clay gel ratio are determined
First, it is 2100 × 10 to load permeability-3μm2Back-up sand rock core；The stratum water of saturation testing block is vacuumized, is counted
Calculate pore volume PV and measure rock core water phase permeability k10(being shown in Table 3)；3rd, prepare the Pseudomonas alcaligenes fermentation of different proportion
Liquid and clay sol solution (being shown in Table 3), stir, and form mixed solution；The mixed solution of the above-mentioned different proportions of 0.01PV is injected,
20d is cultivated after the completion of injection；The water phase permeability k of measure injection different proportion mixed solution rock core11(being shown in Table 3)；Pass through calculating
It is 1 that the fall of core permeability, which obtains Pseudomonas alcaligenes zymotic fluid and clay gel ratio,：When 4, core permeability declines
Amplitude reaches highest, rate of descent 94.5%.
The different proportion Pseudomonas alcaligenes zymotic fluid of table 3 and clay gel core data list
(4) total injection of Pseudomonas alcaligenes zymotic fluid and clay gel is determined
It is 2100 × 10 to load permeability-3μm2Back-up sand rock core；Vacuumize saturation testing block G15Stratum water, calculate
Pore volume and measure rock core water phase permeability k20, it is shown in Table 4；Pseudomonas alcaligenes zymotic fluid and clay sol solution are according to 1：4
After ratio mixing, stir, form mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV), 20d is cultivated after the completion of injection；Determine the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 4；The fall of core permeability is calculated, permeability decrease amplitude is up to injection rate corresponding to 95.3% rock core
0.04PV, therefore, the total injection of Pseudomonas alcaligenes zymotic fluid and clay gel is 0.04PV.
The different proportion Pseudomonas alcaligenes zymotic fluid of table 4 and clay gel core data list
(5) preparation of mixed solution
To experiment block G15Stratum water in add polyacrylamide be used as suspending agent, be configured to mass concentration be 0.2%
Suspension solution；Secondly, clay gel is added into above-mentioned solution, 7 μm of its average grain diameter, is stirred while adding, mixing speed is
250rpm, mixing time 30min, clay gel mass concentration are 1.0%；The production alkali that mixing time terminates rear aforementioned proportion is false single
Born of the same parents' fermented liquid, obtains mixed solution.
(6) field test
High-pressure plunger pump is utilized to be injected from the water injection well of experiment block the mixed solution prepared, injection rate is
4m3/ h, injection rate 0.04PV, it is 6.0 × 103m3；Mixed solution injection after the completion of according to former daily water-injection rate 30% water filling,
30m3/d；Then become a full member normal water filling.Test block G15It is aqueous to drop to 80.2% by testing preceding 97.3%, it have dropped 17.1 hundred
Branch, block day oil-producing from 32t/d rise to 78t/d, amount of increase in production has reached 144%, and field test results are good.
Embodiment 3
Shengli Oil Field block G32For Thief zone water-drive pool, anisotropism is very serious, and permeability extreme difference reaches 1.50,
75 DEG C of reservoir temperature, permeability 3200 × 10-3μm2, pore volume 7.0 × 105m3, daily water-injection rate 200m3/ d, test proparea
Block aqueous 98.0%, block day oil-producing 43t/d.Implement the stifled tune in deep in the block using the method for the present invention, specific steps are such as
Under：
(1) screening of oil reservoir is tested
Test block G32Reservoir temperature<80 DEG C, permeability>1000×10-3μm2, therefore, meet the oil reservoir sieve of the present invention
Select standard.
(1) screening of alkali microorganism is produced
To 100ml experiment blocks G32Stratum water add production alkali microorganism Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose oxygen
Change Bacillus alcaligenes and Pseudomonas alcaligenes, while add nutrients starch 3g/L, Dried Corn Steep Liquor Powder 1.0g/L, diammonium hydrogen phosphate
The common 10ml of 0.3g/L, the pH value for determining solution are respectively 7.0,7.1,6.9 and 7.0；In experiment block G3275 DEG C of bars of reservoir temperature
After cultivating 20d under part, the pH value for determining solution again is respectively：9.5th, 11,10.5 and 9；Filter out in the experiment reservoir condition
It is alcaligenes eutrophus that lower pH value, which changes maximum production alkali microorganism,.
(3) alcaligenes eutrophus zymotic fluid and clay gel ratio are determined
First, it is 3200 × 10 to load permeability-3μm2Back-up sand rock core；Vacuumize saturation testing block G32Stratum water, meter
Calculate pore volume and measure rock core water phase permeability k10, it is shown in Table 5；Prepare the alcaligenes eutrophus zymotic fluid and clay of different proportion
Sol solution (is shown in Table 5), stirs, and forms mixed solution；The mixed solution of the above-mentioned different proportions of 0.01PV is injected, injection is completed
After cultivate 30d；The water phase permeability k of measure injection different proportion mixed solution rock core11, it is shown in Table 5；Finally, by calculating rock core
It is 1 that the fall of permeability, which obtains alcaligenes eutrophus zymotic fluid and clay gel ratio,：When 4, core permeability fall
Reach highest, rate of descent 94.60%.
The different proportion alcaligenes eutrophus zymotic fluid of table 5 and clay gel core data list
(4) total injection of alcaligenes eutrophus zymotic fluid and clay gel is determined
First, it is 3200 × 10 to load permeability-3μm2Back-up sand rock core；Vacuumize saturation testing block G32Stratum water, meter
Calculate pore volume and measure rock core water phase permeability k20, it is shown in Table 6；Alcaligenes eutrophus zymotic fluid and clay sol solution are according to 1：4
Ratio mixing after, stir, formed mixed solution；Injection not same amount (0.01PV, 0.02PV, 0.03PV, 0.04PV,
Mixed solution 0.05PV), 30d is cultivated after the completion of injection；Determine the water phase permeability k of different injection rate mixed solution rock cores21,
It is shown in Table 6；Finally, the fall of core permeability is calculated, permeability decrease amplitude maximum rock core is injection rate 0.03PV, because
This, the total injection of alcaligenes eutrophus zymotic fluid and clay gel is 0.03PV.
The different proportion alcaligenes eutrophus zymotic fluid of table 6 and clay gel core data list
(5) preparation of mixed solution
To experiment block G32Stratum water in add biological polyoses be used as suspending agent, be configured to mass concentration for 0.3% hang
Floating agent solution；Secondly, clay gel is added into above-mentioned solution, 3 μm of its average grain diameter, is stirred while adding, mixing speed is
300rpm, mixing time 50min, clay gel mass concentration are 3.0%；Mixing time terminates really supporting for rear aforementioned proportion and produces alkali
Bacillus fermentation liquid, obtains mixed solution.
(6) field test
High-pressure plunger pump is utilized to be injected from the water injection well of experiment block the mixed solution prepared, injection rate is
5m3/ h, injection rate 0.03PV, it is 2.1 × 103m3；Mixed solution injection after the completion of according to former daily water-injection rate 25% water filling,
75m3/d；Then become a full member normal water filling.Test block G32It is aqueous to drop to 83.5% by testing preceding 98.0%, it have dropped 14.5 hundred
Branch, block day oil-producing from 43t/d rise to 95t/d, amount of increase in production has reached 120.9%, and field test results are good.
Claims (10)
1. one kind adjusts technique using microorganism and the compound deep of clay gel are stifled, it is characterised in that the technique specifically includes following step
Suddenly：
(1) screening of oil reservoir is tested；
(2) screening of alkali microorganism is produced；
(3) production alkali microbial fermentation solution and clay gel ratio are determined；
(4) total injection of production alkali microbial fermentation solution and clay gel is determined；
(5) preparation of mixed solution；
(6) field test.
2. according to claim 1 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
The screening of oil reservoir is tested, specific screening criteria is as follows：Reservoir temperature<80 DEG C, permeability>1000×10-3μm2。
3. according to claim 1 or 2 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that institute
The screening for the production alkali microorganism stated, specific method are as follows：Production alkali microorganism and its battalion are added to the stratum water of 100ml experiment oil reservoirs
The common 10ml of thing is supported, determines the pH value of solution；Determine the pH of solution again after 15~20d is cultivated under the conditions of testing reservoir temperature
Value；Filter out the maximum production alkali microorganism of pH value change.
4. according to claim 3 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
It is one kind in Bacillus foecalis alkaligenes, alcaligenes eutrophus, xylose oxidation Bacillus alcaligenes and Pseudomonas alcaligenes to produce alkali microorganism；Institute
State production alkali microorganism nutrients for 2~5g/L of starch, 0.5~1.0g/L of Dried Corn Steep Liquor Powder, diammonium hydrogen phosphate 0.1~
0.3g/L。
5. according to claim 1 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
The determination method for producing alkali microorganism and clay gel ratio is as follows：Load and experiment Reservoir Permeability identical back-up sand rock core；Take out true
The stratum water of empty saturation testing oil reservoir, calculate pore volume and measure rock core water phase permeability k10；Prepare the production alkali of different proportion
Microbial fermentation solution and clay sol solution, stir, and form mixed solution；The mixing for injecting the above-mentioned different proportions of 0.01PV is molten
Liquid, 15~30d is cultivated after the completion of injection；The water phase permeability k of measure injection different proportion mixed solution rock core11；Calculate rock core
The fall of permeability, the ratio of mixed solution corresponding to permeability decrease amplitude maximum rock core are to determine production alkali microorganism
Zymotic fluid and clay gel ratio.
6. according to claim 1 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
The method for producing the total injection determination of alkali microbial fermentation solution and clay gel is as follows：Load and filled out with experiment Reservoir Permeability identical
The sandstone heart；The stratum water of saturation testing oil reservoir is vacuumized, calculates pore volume and measure rock core water phase permeability k20；Prepare above-mentioned
The production alkali microbial fermentation solution and clay sol solution of ratio, stir, and form mixed solution；It is molten to inject different amounts of mixing
Liquid, 15~30d is cultivated after the completion of injection；Determine the water phase permeability k of different injection rate mixed solution rock cores21；Rock core is calculated to ooze
The fall of saturating rate, the injection rate for choosing mixed solution corresponding to permeability decrease amplitude maximum rock core are to produce alkali microorganism
The total injection of zymotic fluid and clay gel.
7. according to claim 6 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
The total injection for producing alkali microbial fermentation solution and clay gel is 0.01~0.05PV.
8. according to claim 1 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
The preparation of mixed solution, specific method are as follows：Suspending agent is added into the stratum water of experiment oil reservoir, being configured to mass concentration is
0.2~0.5% suspension solution；Secondly, add clay gel into above-mentioned solution, stir while adding, mixing speed be 200~
300rpm, mixing time are 30~60min；Mixing time adds according to aforementioned proportion after terminating and produces alkali microbial fermentation solution, stirs
Mix and uniformly obtain mixed solution.
9. according to claim 8 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
Suspending agent is biological polyoses or polyacrylamide.
10. according to claim 1 block up tune method using microorganism and the compound deep of clay gel, it is characterised in that described
Field test, comprise the following steps that：The mixed solution prepared is utilized into high-pressure plunger pump from the water injection well of experiment block
Injection, injection rate is 3~5m3/h；According to 20~30% water fillings of former daily water-injection rate after the completion of mixed solution injection；Turn after 7d
Normal water filling.
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148303A (en) * | 2006-09-24 | 2008-03-26 | 江苏博大环保股份有限公司 | Oil field re-injection water microbiological treatment |
| US20110146973A1 (en) * | 2009-12-21 | 2011-06-23 | Instituto Mexicano Del Petroleo | Biotechnological process for hydrocarbon recovery in low permeability porous media |
| CN103114833A (en) * | 2011-11-17 | 2013-05-22 | 中国石油化工股份有限公司 | Microbe oil extraction method for activating oil deposit deep portion function flora |
| CN103147731A (en) * | 2013-03-25 | 2013-06-12 | 北京大学 | Method for enhancing crude oil recovery ratio |
| CN103291267A (en) * | 2013-06-14 | 2013-09-11 | 中国石油化工股份有限公司 | Method for improving oil well yields by means of oil pool indigenous microorganisms |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN105201471A (en) * | 2015-09-28 | 2015-12-30 | 中国石油化工股份有限公司 | Microbial enhanced oil recovery method |
| CN105626014A (en) * | 2015-07-29 | 2016-06-01 | 中国石油化工股份有限公司 | Method for improving oil well production through microorganism single well processing |
-
2017
- 2017-08-25 CN CN201710740174.5A patent/CN107476778B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101148303A (en) * | 2006-09-24 | 2008-03-26 | 江苏博大环保股份有限公司 | Oil field re-injection water microbiological treatment |
| US20110146973A1 (en) * | 2009-12-21 | 2011-06-23 | Instituto Mexicano Del Petroleo | Biotechnological process for hydrocarbon recovery in low permeability porous media |
| CN103114833A (en) * | 2011-11-17 | 2013-05-22 | 中国石油化工股份有限公司 | Microbe oil extraction method for activating oil deposit deep portion function flora |
| CN103147731A (en) * | 2013-03-25 | 2013-06-12 | 北京大学 | Method for enhancing crude oil recovery ratio |
| CN103291267A (en) * | 2013-06-14 | 2013-09-11 | 中国石油化工股份有限公司 | Method for improving oil well yields by means of oil pool indigenous microorganisms |
| CN104453811A (en) * | 2014-10-27 | 2015-03-25 | 中国石油化工股份有限公司 | Microbial enhanced oil recovering method of moderate-high permeability reservoir |
| CN105626014A (en) * | 2015-07-29 | 2016-06-01 | 中国石油化工股份有限公司 | Method for improving oil well production through microorganism single well processing |
| CN105201471A (en) * | 2015-09-28 | 2015-12-30 | 中国石油化工股份有限公司 | Microbial enhanced oil recovery method |
Non-Patent Citations (1)
| Title |
|---|
| 汪卫东等: "微生物堵调研究进展", 《油气地质与采收率》 * |
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