CN107475338A - The extracting method of albumen and dietary fiber in a kind of tealeaves - Google Patents
The extracting method of albumen and dietary fiber in a kind of tealeaves Download PDFInfo
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- CN107475338A CN107475338A CN201710712846.1A CN201710712846A CN107475338A CN 107475338 A CN107475338 A CN 107475338A CN 201710712846 A CN201710712846 A CN 201710712846A CN 107475338 A CN107475338 A CN 107475338A
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- tealeaves
- dietary fiber
- albumen
- egg white
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- 238000000034 method Methods 0.000 title claims abstract description 19
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- 108090000623 proteins and genes Proteins 0.000 claims abstract description 17
- 238000000605 extraction Methods 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 10
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- 238000005728 strengthening Methods 0.000 claims description 19
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
- A21D13/064—Products with modified nutritive value, e.g. with modified starch content with modified protein content
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/24—Organic nitrogen compounds
- A21D2/26—Proteins
- A21D2/264—Vegetable proteins
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D2/00—Treatment of flour or dough by adding materials thereto before or during baking
- A21D2/08—Treatment of flour or dough by adding materials thereto before or during baking by adding organic substances
- A21D2/36—Vegetable material
- A21D2/364—Nuts, e.g. cocoa
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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- Health & Medical Sciences (AREA)
- Food Science & Technology (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Nutrition Science (AREA)
- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The present invention provides the extracting method of albumen and dietary fiber in a kind of tealeaves, by being extracted to subtraction, Enzymatic Extraction the methods of, the albumen and dietary fiber of a step.The present invention is using raw material is revealed as, and using ultrasonic technique assisted extraction tea albumen and fiber, the method protein and fiber productivity are high, and purity is high, and method cost is low, simple and easy, easy to spread, and product quality meets food hygienic standard.The invention provides the new way that tealeaves increment conversion recycles；A kind of new plant-derived albumen and diet fiber product are developed, realizes tea resources comprehensive utilization and intensive processing.
Description
Technical field
The invention belongs to biological technical field, and in particular to the extracting method of albumen and dietary fiber in a kind of tealeaves.
Background technology
China is Tea Production big country, and tea industry is quickly grown, and tea place area and yield constantly increase, and tea resource confession occurs
More than the trend asked.Counted according to FAO-IGG, global tea yield reaches 392.84 ten thousand tons within 2009.Nearly 10 years Chinese teas
Leaf-making quantity sustainable growth, between 2000 to 2007, tea yield rises to 1,140,000 tons from 71.93 ten thousand tons, average annual to increase
Rate reached 1,310,000 tons close to 11%, 2009 year, was Chan Cha states maximum in the world.And Tea Consumption was from 2000
45.6 ten thousand tons of increase to 2006 660,000 tons, increase less than 6% every year.Most of low-grade tea Summer-autumn tea is because price
Low, the harvesting reason such as labour cost height causes a large amount of tea resources to stay to adopt and be not used.There is presently no effectively develop
Approach, not only causes the huge waste of tea resource, while is also the very big pollution to environment, therefore a large amount of remaining Summer-autumn teas
It has been processed into urgent problem.And reasonably comprehensively utilize with deep development technology for increasing Tea Industry chain economy
Value and environmental protection play the role of key that is good, and meeting national tea industry development strategy demand.
The content of the invention
It is an object of the invention to provide the extracting method of albumen and dietary fiber in a kind of tealeaves.
To achieve the above object, the present invention adopts the following technical scheme that：
1st, the extracting method of albumen and dietary fiber in a kind of tealeaves, methods described include as follows：
（1）Tealeaves is taken, cleans and drains, after crushing, with mass ratio 1:2-4 and drifted along or through containing 0.05 mol/L Tris- hydrochloride buffers
Close uniformly, be placed in stirring and leaching 12h at 1-4 DEG C, filter, obtain filter residue and leaching liquor；
（2）0.3% NaCl solution, solid-liquid ratio 1 will be added in filter residue:10-15, surpass under 50-100W, 40KHZ~60KHZ
Sound wave extracts 1-2h, and temperature is 10-20 DEG C, and 30min is stood after extraction, adds alkali protease, enzymolysis pH is 8.0, temperature
49 DEG C of degree, enzymolysis time are 5 hours, enzyme-substrate weight proportion is 1:25；Filter residue and filtrate are obtained after filtering, after filtrate centrifugation,
Supernatant is taken, supernatant is placed at 4 DEG C, while stirring the absolute ethyl alcohol of slow precooling, ethanol final concentration is reached 20%, it is quiet
4 h are put, 15 min is centrifuged with 10,000 g, collects precipitation；Precipitation is fully dissolved with 0.02 mol/L Tris- hydrochloride buffers
Afterwards with same concentrations Tris- hydrochloride buffer dialysed overnights, obtained solution is protein crude extract, after freeze-drying, is obtained
Tea egg white powder；
（3）Step（2）The filter residue of acquisition, add the acetic acid that volume fraction is 80%, solid-liquid ratio 1:10, extract 30- at 90 DEG C
60min, after cooling, reagent is removed, residue amylase is 1 in solid-liquid ratio:Digested under 15g/mL, pH10.0, temperature 50 C
2-3h, after boiling water bath younger sister, filtration washing, filter residue is collected, tealeaves dietary fiber is produced after drying.
The application of tea egg white powder and dietary fiber in the biscuit with stomach strengthening and digestion promoting effect is prepared.
The biscuit includes following raw material：Peanut red coat 10-15 parts, Olive leaf P.E 5-8 parts, tea leaf protein
Powder 3-6 parts, tealeaves dietary fiber 3-6 parts, cashew nut 3-7 parts, wheat flour 80-120 parts, egg white 40-50 parts, milk powder 10-20 parts, hair
Ferment powder 1-3 parts, water 30-50 parts.
Dietary fiber tool has obvious prevention in human body to a variety of diseases such as diabetes, cardiovascular disease, obesity, constipation
And therapeutic action.Tea leaf protein be it is a kind of have the protein with plurality of health care functions with nutrition, itself is nontoxic, and it has
Remove superoxide anion, radioresistance, reducing blood lipid, anti-mutation and other effects.
The advantage of the invention is that：
The present invention obtains tea albumen and fiber using one-step method extraction, greatly reduces the waste of raw material, is extracted using salt, subtraction
Extraction and alkali proteinase method be combined with each other, and the protein delivery in tealeaves is improved hot bath alkaline process into extract solution
The traditional handicraft of albumen is extracted, the drawbacks of alkali lye consumption amount is big, extraction rate of protein is low is overcome, with traditional hot bath alkaline process
Extraction process is compared, and improves the recovery rate of protein and the purity of albumen, and the recovery rate of albumen is up to more than 90%, tea egg
Lipidated protein is up to more than 80% in white extract.In addition, the present invention is combined with amylase using acetic acid and prepares cellulose,
The integrity degree of cellulosic structure is improved, and the recovery rate of cellulose can reach more than 96%, than cellulose in the prior art
Extracting method efficiency high and simplicity.
In addition, albumen powder and dietary fiber that the present invention is obtained using extraction, with peanut red coat, Olive leaf P.E, waist
Fruit is combined, and optimal raw material and content is determined by the experiment of applicant, prepares the cake with stomach strengthening and digestion promoting effect
It is dry, wherein five kinds of raw materials are also strictly to consider state of an illness weight and the amount of medicine needed for symptom difference according to instruction of Chinese Medicine theory
In the case of difference, it is contemplated that the complexity of Chinese medicine efficacy effect, it is determined that collaboration and antagonism between each raw material, lead to
Experiment many times is crossed, a kind of composition safe and non-toxic, that there is stomach strengthening and digestion promoting and other effects is obtained, is added to one made in biscuit
Kind novel healthy food, is suitable for each age level and eats.
Embodiment
Embodiment 1
（1）Tealeaves is taken, cleans and drains, after crushing, with mass ratio 1:3 and close containing 0.05 mol/L Tris- hydrochloride buffers are dark
Uniformly, stirring and leaching 12h at 3 DEG C is placed in, filters, obtains filter residue and leaching liquor；
（2）0.3% NaCl solution, solid-liquid ratio 1 will be added in filter residue:12, the ultrasonic-leaching 1-2h under 80W, 50KHZ, temperature
Spend for 15 DEG C, 30min is stood after extraction, add alkali protease, enzymolysis pH is 8.0,49 DEG C of temperature, enzymolysis time 5
Hour, enzyme-substrate weight proportion are 1:25；Filter residue and filtrate are obtained after filtering, after filtrate centrifugation, supernatant is taken, by supernatant
Be placed at 4 DEG C, while stirring the absolute ethyl alcohol of slow precooling, ethanol final concentration is reached 20%, stand 4 h, with 10,000 g from
The min of the heart 15, collect precipitation；Precipitate after fully being dissolved with 0.02 mol/L Tris- hydrochloride buffers with same concentrations Tris- salt
Acid buffer dialysed overnight, obtained solution is protein crude extract, after freeze-drying, the tea egg white powder of acquisition；
（3）Step（2）The filter residue of acquisition, add the acetic acid that volume fraction is 80%, solid-liquid ratio 1:10, extracted at 90 DEG C
45min, after cooling, reagent is removed, residue amylase is 1 in solid-liquid ratio:Digested under 15g/mL, pH10.0, temperature 50 C
3h, after boiling water bath younger sister, filtration washing, filter residue is collected, tealeaves dietary fiber is produced after drying.
Tea protein extracting ratio：94%, protein content in tea protein extract：90%.
Cellulose recovery rate：97%.
Tea egg white powder and dietary fiber prepare the biscuit with stomach strengthening and digestion promoting effect, and the biscuit includes following parts by weight
Raw material：13 parts of peanut red coat, 7 parts of Olive leaf P.E, 4 parts of tea egg white powder, 5 parts of tealeaves dietary fiber, 5 parts of cashew nut, wheat
100 parts of powder, 45 parts of egg white, 15 parts of milk powder, 2 parts of yeast powder, 40 parts of water.Prepare according to a conventional method.
Embodiment 2
（1）Tealeaves is taken, cleans and drains, after crushing, with mass ratio 1:2 and close containing 0.05 mol/L Tris- hydrochloride buffers are dark
Uniformly, stirring and leaching 12h at 1 DEG C is placed in, filters, obtains filter residue and leaching liquor；
（2）0.3% NaCl solution, solid-liquid ratio 1 will be added in filter residue:10, the ultrasonic-leaching 1h under 50W, 40KHZKHZ,
Temperature is 10 DEG C, and 30min is stood after extraction, adds alkali protease, enzymolysis pH is 8.0,49 DEG C of temperature, enzymolysis time are
5 hours, enzyme-substrate weight proportion be 1:25；Filter residue and filtrate are obtained after filtering, after filtrate centrifugation, supernatant is taken, by supernatant
Be placed at 4 DEG C, while stirring the absolute ethyl alcohol of slow precooling, ethanol final concentration is reached 20%, stand 4 h, with 10,000 g from
The min of the heart 15, collect precipitation；Precipitate after fully being dissolved with 0.02 mol/L Tris- hydrochloride buffers with same concentrations Tris- salt
Acid buffer dialysed overnight, obtained solution is protein crude extract, after freeze-drying, the tea egg white powder of acquisition；
（3）Step（2）The filter residue of acquisition, add the acetic acid that volume fraction is 80%, solid-liquid ratio 1:10, extracted at 90 DEG C
30min, after cooling, reagent is removed, residue amylase is 1 in solid-liquid ratio:Digested under 15g/mL, pH10.0, temperature 50 C
2h, after boiling water bath younger sister, filtration washing, filter residue is collected, tealeaves dietary fiber is produced after drying.
Tea protein extracting ratio：91.5%, protein content in tea protein extract：87%.
Cellulose recovery rate：96%.
Tea egg white powder and dietary fiber prepare the biscuit with stomach strengthening and digestion promoting effect, and the biscuit includes following parts by weight
Raw material：10 parts of peanut red coat, 5 parts of Olive leaf P.E, 3 parts of tea egg white powder, 3 parts of tealeaves dietary fiber, 3 parts of cashew nut, wheat
80 parts of powder, 40 parts of egg white, 10 parts of milk powder, 1 part of yeast powder, 30 parts of water.
Embodiment 3
（1）Tealeaves is taken, cleans and drains, after crushing, with mass ratio 1:4 and drifted along or through containing 0.05 mol/L Tris- hydrochloride buffers
Close uniformly, be placed in stirring and leaching 12h at 4 DEG C, filter, obtain filter residue and leaching liquor；
（2）0.3% NaCl solution, solid-liquid ratio 1 will be added in filter residue:15, the ultrasonic-leaching 1h under 100W, 460KHZ,
Temperature is 20 DEG C, and 30min is stood after extraction, adds alkali protease, enzymolysis pH is 8.0,49 DEG C of temperature, enzymolysis time are
5 hours, enzyme-substrate weight proportion be 1:25；Filter residue and filtrate are obtained after filtering, after filtrate centrifugation, supernatant is taken, by supernatant
Be placed at 4 DEG C, while stirring the absolute ethyl alcohol of slow precooling, ethanol final concentration is reached 20%, stand 4 h, with 10,000 g from
The min of the heart 15, collect precipitation；Precipitate after fully being dissolved with 0.02 mol/L Tris- hydrochloride buffers with same concentrations Tris- salt
Acid buffer dialysed overnight, obtained solution is protein crude extract, after freeze-drying, the tea egg white powder of acquisition；
（3）Step（2）The filter residue of acquisition, add the acetic acid that volume fraction is 80%, solid-liquid ratio 1:10, extracted at 90 DEG C
60min, after cooling, reagent is removed, residue amylase is 1 in solid-liquid ratio:Digested under 15g/mL, pH10.0, temperature 50 C
2-3h, after boiling water bath younger sister, filtration washing, filter residue is collected, tealeaves dietary fiber is produced after drying.
Tea protein extracting ratio：92%, protein content in tea protein extract：88.5%.
Cellulose recovery rate：96.5%.
Tea egg white powder and dietary fiber prepare the biscuit with stomach strengthening and digestion promoting effect, and the biscuit includes following parts by weight
Raw material：15 parts of peanut red coat, 8 parts of Olive leaf P.E, 6 parts of tea egg white powder, 6 parts of tealeaves dietary fiber, 7 parts of cashew nut, wheat
120 parts of powder, 50 parts of egg white, 20 parts of milk powder, 3 parts of yeast powder, 50 parts of water.
Application examples 1
The present invention is used for the toxicity test of the pharmaceutical composition of stomach strengthening and digestion promoting
Maximal tolerance dose is administered in Mouse oral（MID）For 24g/kg, equivalent to the 565 of the maximum usage amount on the one of human body in clinic
Times；Using obtained composition in embodiment 1（13 parts of peanut red coat, 7 parts of Olive leaf P.E, 4 parts of tea egg white powder, tealeaves
5 parts of dietary fiber, 5 parts of cashew nut）, after carrying out gastric infusion to mouse, 7 days Continuous Observations have no animal dead, activity freely,
Feed drinking-water and stool and urine are normal, and ordinary circumstance is good, and abnormal response is not observed, and has no obvious acute toxic reaction.
The composition for stomach strengthening and digestion promoting in embodiment 1 is subjected to gavage to rat with 4g/kg, 8g/kg dosage, while set just
Normal control group, successive administration 30 days；During administration situations such as the external appearance characteristic of record rat, diet, excrement, body weight；As a result
Show that the rat of administration 30 days is without exception compared with Normal group, hematological indices, blood parameters are examined
Survey, autopsy is observed, internal organs histopathologic inspection result indifference compared with Normal group.
Application examples 2
In tests below example, A represents 13 parts of peanut red coat, 7 parts of Olive leaf P.E, is used for stomach invigorating made from 5 parts of cashew nut
The composition to help digestion；B represents 4 parts of tea egg white powder, the composition for stomach strengthening and digestion promoting of 5 parts of tealeaves dietary fiber；C tables
Show 13 parts of peanut red coat, 7 parts of Olive leaf P.E, 4 parts of tea egg white powder, 5 parts of tealeaves dietary fiber, use made from 5 parts of cashew nut
In the composition of stomach strengthening and digestion promoting.
Test objective：By animal pharmacodynamic study, verify that the pharmaceutical composition A for stomach strengthening and digestion promoting, B, C are faced
Bed treatment indigestion, inflatable are satisfied the effect of vexed, dyspeptic abdominalgia, fullness sensation in chest abdominal distension, and compare the power of its drug effect.
Test medicine：The preparation of pharmaceutical composition A, B, C for stomach strengthening and digestion promoting.
Dosage is set：Pharmaceutical composition A, B, C for stomach strengthening and digestion promoting set respectively two dosage 2.5g/kg of rat and
5.0g/kg 。
Medication：Using gastric infusion mode.
1st, influence of the composition to rat hydrochloric acid in gastric juice and pepsin activity for stomach strengthening and digestion promoting
Healthy SD rat 70 is taken, male and female have both.160 ~ 190g of body weight, it is randomly divided into seven groups.Fasting 24 hours before experiment.Second
Ehterization, osculum is cut off along ventrimeson, find out stomach ligation pylorus, then according to dosage give equivalent by duodenal administration, control group
Distilled water, suturing them wound.Fu jie of being taken out stitches out after 3 hours pricks orifice of the stomach, extracts full stomach, cuts off coat of the stomach along greater curvature, pour out stomach
Content, it is collected in graduated centrifuge tube, is centrifuged 8 minutes with 3000r/min rotating speeds, it is stand-by takes out supernatant.
The measure of total acidity gastric juice：
Gastric juice 0.5ml is taken respectively, is put in conical flask and is titrated with 0.02N NaOH, using phenolphthalein as indicator, record titration institute
The NaOH amounts spent, total acidity is calculated with above formula, then take 1ml gastric juice to put in conical flask, be put into two and be full of albumen
Capillary, it is incubated 24 hours at a temperature of 37 DEG C, measures the length of the transparent part in glass capillary both ends（mm）, with the flat of four ends
Average represents the active unit of pepsin, and statistical result is shown in Table 1.
Table 1 is used for influence of the composition of stomach strengthening and digestion promoting to rat hydrochloric acid in gastric juice total acidity and pepsin activity
* is with comparing P ＜ 0.01, * P ＜ 0.05
Result of the test shows：：C can increase rat total acidity gastric juice, strengthen pepsin activity, and the effect of high dose group is notable,
Low dose group then acts on unobvious in terms of pepsin activity enhancing, with control group than P ＜ 0.05.The effect that two groups of AB is not
Significantly.
2nd, the composition for stomach strengthening and digestion promoting is to the analgesic activity of mouse
Healthy mice 70 is taken, 18 ~ 22g of body weight, male and female half and half, is randomly divided into seven groups.Control group gives isometric steaming
Distilled water.After administration 60 minutes, the acetate solution 0.2ml/ of each mouse intraperitoneal injection 0.6% only, is observed and to record injection acetic acid molten
There is the number of writhing response in mouse in 20 minutes after liquid, calculates analgesia of the pharmaceutical composition for stomach strengthening and digestion promoting to mouse
Percentage, it the results are shown in Table 2.
Table 2 is used for analgesic activity of the composition to mouse of stomach strengthening and digestion promoting
* is with comparing P ＜ 0.01, * P ＜ 0.05
Result of the test shows：C has significant analgesic activity, the effect that two groups of AB does not show to the writhing caused by mouse acetic acid
Write.
The foregoing is only presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with
Modification, it should all belong to the covering scope of the present invention.
Claims (4)
- A kind of 1. extracting method of albumen and dietary fiber in tealeaves, it is characterised in that：Methods described includes as follows：（1）Tealeaves is taken, cleans and drains, after crushing, with mass ratio 1:2-4 and drifted along or through containing 0.05 mol/L Tris- hydrochloride buffers Close uniformly, be placed in stirring and leaching 12h at 1-4 DEG C, filter, obtain filter residue and leaching liquor；（2）0.3% NaCl solution, solid-liquid ratio 1 will be added in filter residue:10-15, surpass under 50-100W, 40KHZ~60KHZ Sound wave extracts 1-2h, and temperature is 10-20 DEG C, and 30min is stood after extraction, adds alkali protease, enzymolysis pH is 8.0, temperature 49 DEG C of degree, enzymolysis time are 5 hours, enzyme-substrate weight proportion is 1:25；Filter residue and filtrate are obtained after filtering, after filtrate centrifugation, Supernatant is taken, supernatant is placed at 4 DEG C, while stirring the absolute ethyl alcohol of slow precooling, ethanol final concentration is reached 20%, it is quiet 4 h are put, 15 min is centrifuged with 10,000 g, collects precipitation；Precipitation is fully dissolved with 0.02 mol/L Tris- hydrochloride buffers Afterwards with same concentrations Tris- hydrochloride buffer dialysed overnights, obtained solution is protein crude extract, after freeze-drying, is obtained Tea egg white powder；（3）Step（2）The filter residue of acquisition, add the acetic acid that volume fraction is 80%, solid-liquid ratio 1:10, extract 30- at 90 DEG C 60min, after cooling, reagent is removed, residue amylase is 1 in solid-liquid ratio:Digested under 15g/mL, pH10.0, temperature 50 C 2-3h, after boiling water bath younger sister, filtration washing, filter residue is collected, tealeaves dietary fiber is produced after drying.
- 2. a kind of extracting method as claimed in claim 1 extracts obtained tea egg white powder and dietary fiber.
- 3. tea egg white powder as claimed in claim 2 and dietary fiber answering in the biscuit with stomach strengthening and digestion promoting effect is prepared With.
- 4. application according to claim 1, it is characterised in that：The biscuit includes following raw material：Peanut red coat 10-15 parts, Olive leaf P.E 5-8 parts, tea egg white powder 3-6 parts, tealeaves dietary fiber 3-6 parts, cashew nut 3-7 parts, wheat flour 80-120 parts, egg white 40-50 parts, milk powder 10-20 parts, yeast powder 1-3 parts, water 30-50 parts.
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