CN107475094B - Blood cell separation tube and separation method - Google Patents
Blood cell separation tube and separation method Download PDFInfo
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- CN107475094B CN107475094B CN201710686598.8A CN201710686598A CN107475094B CN 107475094 B CN107475094 B CN 107475094B CN 201710686598 A CN201710686598 A CN 201710686598A CN 107475094 B CN107475094 B CN 107475094B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0634—Cells from the blood or the immune system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
- C12N2509/10—Mechanical dissociation
Abstract
The invention belongs to the technical field of blood cell separation equipment, and particularly discloses a blood cell separation tube and a separation method. When the blood separation tube transfers anticoagulation, the anticoagulation bottle is connected with the centrifuge tube, only the anticoagulation enters the flexible liquid storage bag under the action of the filter screen, and glass beads are not allowed to pass through, so that the anticoagulation can be transferred while shaking the bottle until all the anticoagulation is transferred, the whole process does not need to be absorbed for multiple times, and the operation is simple.
Description
Technical Field
The invention belongs to the technical field of blood cell separation equipment, and particularly relates to a blood cell separation tube and a separation method.
Background
Blood detection and medical diagnosis are important in blood cell separation. After blood is collected, anticoagulant treatment is firstly carried out by using reagents such as heparin or EDTA (ethylene diamine tetraacetic acid) and the like, so that blood coagulation is avoided, and in order to rapidly mix and dissolve the anticoagulant reagents such as heparin and the like with newly collected blood, a small glass bead shaking mode is generally adopted. Although this method is somewhat cumbersome to perform, it is often used in blood cell separation because it has minimal effect on lymphocyte activity and can reduce platelet contamination. After the collected blood is anticoagulated, blood cell separation is started, for example, a centrifugal separation method is adopted for separating lymphocytes, a layering liquid is firstly placed in a centrifugal tube, anticoagulated blood is sucked and added to the layering liquid, liquid in the centrifugal tube is layered after centrifugation, a mononuclear cell layer is positioned in an intermediate layer, and the layer of cells is also one of important objects of blood detection.
However, in the prior art, when the blood is anticoagulated by a small glass bead shake flask and then transferred to a centrifuge tube for centrifugal separation, the blood is generally directly poured because the shake flask and the centrifuge tube are in a separated state, and the anticoagulated blood in the shake flask cannot be completely collected; if the anticoagulation blood is sucked by the suction device and then transferred into the centrifugal tube, the anticoagulation blood needs to be sucked for a plurality of times, and the operation is troublesome. Therefore, there is a need for improvements to existing blood separation shake flasks and centrifuge tubes.
Disclosure of Invention
The invention aims to provide a blood cell separation tube and a separation method, which solve the problem that in the prior art, blood samples in a shake flask cannot be completely collected because the shake flask and a centrifuge tube are in a separation state; the anticoagulation blood transfer needs to be performed by multiple times of suction, and the operation is troublesome.
The invention provides a blood cell separation tube, which comprises a centrifuge tube, an anticoagulation bottle with openings at the upper end and the lower end, and a connector positioned between the centrifuge tube and the anticoagulation bottle, wherein a filter screen is arranged at the opening at the bottom of the anticoagulation bottle, the connector comprises a connecting shell, a piston and a four-way liquid distribution tube, the four-way liquid distribution tube is positioned in the connecting shell, the upper pipe orifice and the lower pipe orifice of the four-way liquid distribution tube are respectively communicated with the filter screen and a liquid outlet arranged at the bottom of the connecting shell, one end of the piston which penetrates through the side surface of the connecting shell is rotatably plugged between the left pipe orifice and the right pipe orifice of the four-way liquid distribution tube, a liquid flow channel is longitudinally arranged on the piston, and a connecting tube which;
the centrifuging tube includes the outer tube and is located transparent "the" shape inner tube of zigzag "in the outer tube, just connect the upper and lower end of casing detachable respectively and connect the bottom opening part of anti-freezing bottle with on the outer tube mouth of pipe, the lower extreme mouth of pipe of connecting pipe peg graft in the upper end opening of" the "shape inner tube of zigzag", the "shape inner tube of zigzag" is formed by the concatenation of a plurality of slope straight tubes, and the upper surface of every slope straight tube has seted up a plurality of pinholes, the inside flexible stock solution bag of having laid along pipeline direction of "the" shape inner tube of zigzag ", still be provided with the outer tube cap on the outer tube, be provided with the inner tube cap on" the "shape inner tube of zigzag".
Preferably, the diameter of the mesh of the filter screen of the blood cell separation tube is less than or equal to 2 mm.
Preferably, the blood cell separator tube, the upper and lower extreme of connecting the casing is respectively the spiral shell be in the lower extreme opening part of anticoagulation bottle with on the outer tube mouth of pipe, and the upper end of connecting the casing with the lower extreme opening of anticoagulation bottle the lower extreme of connecting the casing with all be equipped with sealed the pad between the outer tube.
Preferably, the blood cell separation tube, the piston and the left end tube and the right end tube of the four-way liquid distribution tube are coated with lubricating and sealing agents.
Preferably, the blood cell separation tube and the lubricating sealant are vaseline.
Preferably, the blood cell separation pipe, the connecting pipe is flat syringe needle shape down, and the outside parcel of upper end of connecting pipe has the rubber buffer, and the rubber buffer with the contact of the lower extreme inner wall of four-way branch liquid pipe is connected.
Preferably, the blood cell separation tube, between the outer wall of the inner tube and the inner wall of the outer tube, is filled with a buffer.
Preferably, the blood cell separation tube and the buffer are sponge, cotton or absorbent cotton.
Preferably, the blood cell separation tube and the flexible liquid storage bag are transparent bags made of flexible materials.
The invention also provides a method for separating blood cells by using the blood cell separation tube, which comprises the following steps:
s1, collecting fresh blood, placing the fresh blood in an anticoagulation bottle filled with glass beads, adding the blood anticoagulation agent, and shaking the bottle to obtain anticoagulation;
and S2, transferring the anticoagulation blood into a flexible liquid storage bag filled with layering liquid through a connector, and centrifuging to obtain layered blood cell solution so as to complete separation of blood cells.
Compared with the prior art, the blood cell separation tube and the separation method have the following beneficial effects:
1. when the blood separation tube transfers anticoagulation, the anticoagulation bottle is connected with the centrifuge tube, only the anticoagulation enters the flexible liquid storage bag under the action of the filter screen, and glass beads are not allowed to pass through, so that the anticoagulation can be transferred while shaking the bottle until all the anticoagulation is transferred, the whole process does not need to be absorbed for multiple times, and the operation is simple.
2. The flexible liquid storage bag belongs to a long and thin shape, so the length of the middle-layer liquid is enlarged, the liquid taking operation is performed on the flexible liquid storage bag, in addition, a needle hole is formed in the zigzag inner tube, the suction device punctures into the flexible liquid storage bag along the needle hole, the middle-layer liquid is directly sucked, the trouble that the middle-layer liquid can be sucked by sucking the upper-layer liquid firstly after the separation of the traditional centrifugal tube is avoided, and the phenomenon that the upper-layer liquid pollutes the middle-layer liquid when the suction device directly penetrates through the upper-layer liquid to suck the middle-layer liquid is also avoided.
3. Set up a plurality of pinholes, can select the pinhole puncture that is closest to middle level solution bottom according to actual operation, once puncture can absorb middle level solution completely, the flexibility is strong.
Drawings
FIG. 1 is a schematic structural view of a blood cell separation tube according to the present invention;
FIG. 2 is a schematic view of the structure of the connection housing in the blood cell separation tube according to the present invention;
FIG. 3 is a schematic view showing the structure of a zigzag-shaped inner tube in the blood cell separation tube according to the present invention;
FIG. 4 is a schematic view of the structure of the centrifuge tube in the blood cell separation tube according to the present invention.
In the figure, 1, an anticoagulant bottle, 11, a filter screen, 2, a connecting shell, 21, a piston, 211, a flow channel, 22, a four-way liquid distribution pipe, 23, a connecting pipe, 24, a rubber plug, 3, an outer pipe, 31, an outer pipe cover, 4, a zigzag inner pipe, 41, a needle hole, 42, an inner pipe cover, 5, a flexible liquid storage bag and 6, a buffer are arranged.
Detailed Description
The following detailed description of the present invention is provided in conjunction with the accompanying drawings, but it should be understood that the scope of the present invention is not limited to the specific embodiments.
A blood cell separation tube and a separation method are disclosed, as shown in figure 1-4, comprising a centrifuge tube, an anticoagulation bottle 1 with openings at the upper and lower ends and a connector between the centrifuge tube and the anticoagulation bottle 1, wherein the opening at the bottom of the anticoagulation bottle 1 is provided with a filter screen 11, the connector comprises a connecting shell 2, a piston 21 and a four-way liquid distribution tube 22, the four-way liquid distribution tube 22 is provided with an upper tube, a lower tube, a left tube and a right tube, the upper and lower surfaces of the connecting shell 2 are provided with liquid outlets, the side surface can be provided with a penetration port, the four-way liquid distribution tube 22 is positioned in the connecting shell 2, the upper tube port of the four-way liquid distribution tube 22 is communicated with the filter screen 11, the inner diameter of the upper tube port is equal to the outer diameter of the filter screen 11, the lower tube port of the four-way liquid distribution tube 22 is communicated with the liquid outlet arranged at the bottom of, the other end is left outside the connecting shell 2, a liquid flow channel 211 is longitudinally arranged on the piston 21, the liquid flow channel 211 can rotate to be respectively communicated with the two openings at the upper end and the lower end of the four-way liquid distributing pipe 22 and is used for anticoagulation blood to pass through, and can also rotate to be not completely communicated with the two openings at the upper end and the lower end of the four-way liquid distributing pipe 22 and to ensure anticoagulation blood not to pass through, and a connecting pipe 23 which can move up and down is sleeved in the lower end pipe (the part below the piston 21) of the four-way liquid;
the centrifuging tube includes upper end open-ended outer tube 3 and is located the transparent "zigzag" shape inner tube 4 of upper end open-ended in the outer tube 3, and connect the casing 2 on, the lower extreme is detachable respectively and connects on the bottom opening part of anticoagulation bottle 1 and the 3 mouth of pipe of outer tube, the lower extreme mouth of pipe of connecting pipe 23 is pegged graft in the upper end opening of "zigzag" shape inner tube 4, "zigzag" shape inner tube 4 is formed by the concatenation of a plurality of slope straight tubes, and a plurality of pinholes 41 have been seted up to the upper surface of every slope straight tube, "flexible stock solution bag 5 has been laid along the pipeline direction to zigzag" shape inner tube 4 inside, outer tube cover 31 that can seal outer tube 3 is still spiro union on the outer tube 3, it has the inner tube cap 42 that can seal "zigzag" shape inner tube 4 still to spiro union on.
The diameter of the mesh of the filter screen 11 is less than or equal to 2mm, and the length and the width of the four-way liquid distribution pipe 22 are both less than or equal to 1 cm; the upper end and the lower end of the connecting shell 2 are both sunken towards the middle part, the upper end sunken part is in bolt connection with the anticoagulation bottle 1, and the lower end sunken part is in bolt connection with the outer tube 3; the length of the connecting pipe 23 is less than the sum of the length of the pipe at the lower end of the four-way liquid distributing pipe 22 and the depth of the recess at the lower end of the connecting shell 2, so that the connecting pipe 23 can be pushed into the pipe orifice at the lower end of the four-way liquid distributing pipe 22 and the recess at the lower end of the connecting shell 2.
Connect the upper and lower extreme of casing 2 spiral shell respectively at the lower extreme opening part of anticoagulation bottle 1 and the upper end mouth of pipe department of outer tube 3 to connect and all be equipped with sealed the pad between the upper end of casing 2 and the lower extreme opening of anticoagulation bottle 1, the upper end mouth of pipe of lower extreme and outer tube 3, connect on the lower extreme opening part of anticoagulation bottle 1 and outer tube 3 with the upper and lower extreme detachable that realizes connecting the casing.
The lubricating sealant vaseline is coated between the left end pipe and the right end pipe of the piston 21 and the four-way liquid distribution pipe 22, so that the sealing performance and the lubricating degree are improved, the rotation resistance of the piston is reduced, and anticoagulation blood is prevented from seeping out from the left pipe orifice and the right pipe orifice.
Connecting pipe 23 is flat-top down sharp syringe needle shape, is convenient for peg graft like in the flexible liquid storage bag 5 to the outside parcel in upper end of connecting pipe 23 has rubber buffer 24, and rubber buffer 24 and the lower extreme inner wall in close contact with of cross branch liquid pipe 22 are connected, when guaranteeing that connecting pipe 23 promotes from top to bottom, are unlikely to totally follow the lower extreme landing of cross branch liquid pipe 22.
The buffer 6 is filled between the outer wall of the zigzag inner tube 4 and the inner wall of the outer tube 3, the buffer is sponge, cotton or absorbent cotton, and the collision between the zigzag inner tube 4 and the outer tube 3 is reduced during centrifugation.
The flexible liquid storage bag 5 is a transparent bag made of flexible materials, such as a transparent blood bag mainly made of PVC materials, the thickness of the transparent bag is 0.1-0.3mm, the puncture is convenient, and the price is low.
The method for separating the blood cells by using the blood cell separation tube comprises the following steps:
s1, firstly, rotating the piston 21 to ensure that the flow channel 211 is not communicated with the two openings at the upper end and the lower end of the four-way liquid distributing pipe 22 completely, ensuring that anticoagulation blood cannot pass through, meanwhile, pushing the connecting pipe 23 to be positioned in the lower end pipe of the four-way liquid distributing pipe 22 and not communicated with a centrifuge tube, as shown in figure 2, collecting fresh blood, placing the collected fresh blood in an anticoagulation bottle 1 filled with glass beads, adding blood anticoagulation agents (heparin or EDTA and other reagents), and shaking the bottle to obtain anticoagulation blood;
s2, the connecting pipe 23 is pulled out to be communicated with the zigzag-shaped inner pipe 4, the piston 21 is rotated to enable the fluid channel 211 to be communicated with the two openings at the upper end and the lower end of the four-way fluid distribution pipe 22 to ensure the anticoagulation fluid to pass through, the anticoagulation fluid is transferred into the flexible fluid storage bag 5 filled with the layering fluid through the connecting head by rotating the piston 21 to adjust the flow rate, as shown in figure 1, the anticoagulation fluid is centrifuged to obtain the layered blood cell solution, and the separation of the blood cells is completed.
The stratified fluid adopts the Ficoll stratified fluid method and Percoll stratified fluid which are commonly used in the existing blood cell separation, after the stratification, lymphocytes and the like are positioned between the plasma and the stratified fluid, the outer tube cover 31 and the inner tube cover 42 are respectively covered during the centrifugation, and the flexible liquid storage bag 5 is clamped between the zigzag inner tube 4 and the inner tube cover 42 when the inner tube cover 42 is covered, as shown in figure 4.
When the blood separating tube provided by the invention transfers anticoagulation, the anticoagulation bottle 1 is connected with a centrifugal tube, only anticoagulation is allowed to enter the flexible liquid storage bag 5 under the action of the filter screen 11, and glass beads are not allowed to pass through, so that anticoagulation can be transferred while shaking the bottle until all anticoagulation is transferred, the whole process does not need to be sucked for many times, and the operation is simple.
"zigzag" shape inner tube 4 elongated shape, the internal diameter is showing and is less than outer tube 3 (here outer tube 3 acts as the effect of ordinary centrifuging tube), flexible stock solution bag 5 that so laid along it also belongs to elongated shape, so enlarge the length of middle level solution, the liquid operation is got to the aspect, in addition, pinhole 41 has been seted up on "zigzag" shape inner tube 4, the aspirator punctures into flexible stock solution bag 5 along pinhole 41 in, directly absorb middle level liquid, need suck away upper liquid earlier after avoiding adopting traditional centrifuging tube separation like this, just can absorb the trouble of middle level liquid. Pinhole 41 is only located the upper surface of slope straight tube because under the action of gravity, the effort that middle level liquid downward sloping straight tube lower surface removed is bigger, reduces the risk of overflowing from the upper surface, moreover, sets up a plurality of pinholes 41, can select the pinhole 41 puncture that is closest to middle level solution bottom according to actual operation, and middle level solution can be absorb completely in a puncture, and the flexibility is strong.
It should be noted that, unless otherwise specified, the above components are made of transparent materials, such as glass or plastic, for example, to observe the anticoagulation inside the device.
It should be noted that when the following claims refer to numerical ranges, it should be understood that both ends of each numerical range and any value between the two ends can be selected, and since the steps and methods used are the same as those of the above embodiments, the preferred embodiments of the present invention have been described for the purpose of preventing redundancy, but once the basic inventive concept is known, those skilled in the art may make other variations and modifications to these embodiments. Therefore, it is intended that the appended claims be interpreted as including preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various changes and modifications may be made in the present invention without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include such modifications and variations.
Claims (10)
1. A hemocyte separation tube is characterized by comprising a centrifuge tube, an anticoagulation bottle (1) with openings at the upper end and the lower end and a connector positioned between the centrifuge tube and the anticoagulation bottle (1), the opening at the bottom of the anticoagulation bottle (1) is provided with a filter screen (11), the connector comprises a connecting shell (2), a piston (21) and a four-way liquid distributing pipe (22), the four-way liquid distributing pipe (22) is positioned in the connecting shell (2), and the upper and lower pipe orifices of the four-way liquid distribution pipe (22) are respectively communicated with the filter screen (11) and a liquid outlet arranged at the bottom of the connecting shell (2), one end of the piston (21) penetrates through the side surface of the connecting shell (2), and is rotatably plugged between the left and right pipe orifices of the four-way liquid distribution pipe (22), a liquid flow channel (211) is longitudinally arranged on the piston (21), a connecting pipe (23) which can move up and down is sleeved in the lower end pipe of the four-way liquid distribution pipe (22); the centrifuging tube includes outer tube (3) and is located transparent "zigzag" shape inner tube (4) in outer tube (3), just connect the upper and lower end of casing (2) and connect detachable respectively in the bottom opening part of anticoagulation bottle (1) with on the outer tube (3) mouth of pipe, the lower extreme mouth of pipe of connecting pipe (23) peg graft in the upper end opening of "zigzag" shape inner tube (4), "zigzag" shape inner tube (4) is formed by a plurality of slope straight tube concatenations, and a plurality of pinholes (41) have been seted up to the upper surface of every slope straight tube, flexible stock solution bag (5) have been laid along the pipeline direction to "zigzag" shape inner tube (4) inside, be provided with outer tube cap (31) on outer tube (3), still be provided with inner tube cap (42) on "zigzag" shape inner tube (4).
2. The blood cell separation tube according to claim 1, wherein the mesh diameter of the filter net (11) is not more than 2 mm.
3. The blood cell separation tube of claim 1, wherein the upper and lower ends of the connection housing (2) are respectively screwed on the lower opening of the anticoagulation bottle (1) and the tube opening of the outer tube (3), and sealing gaskets are respectively arranged between the upper end of the connection housing (2) and the lower opening of the anticoagulation bottle (1), the lower end of the connection housing (2) and the centrifuge tube.
4. The blood cell separation tube of claim 1, wherein a lubricant sealant is applied between the piston (21) and the left and right end tubes of the four-way liquid distribution tube (22).
5. The blood cell separation tube according to claim 4, wherein the lubricating sealant is vaseline.
6. The blood cell separation tube according to claim 1, wherein the connection tube (23) has a needle shape with a flat upper end and a pointed lower end, and a rubber stopper (24) is wrapped outside the upper end of the connection tube (23), and the rubber stopper (24) is in contact connection with the inner wall of the lower end of the four-way liquid distribution tube (22).
7. The blood cell separation tube according to claim 1, wherein a buffer (6) is filled between the outer wall of the inner tube (4) and the inner wall of the outer tube (3).
8. The blood cell separation tube according to claim 7, wherein the buffer is sponge or cotton.
9. The blood cell separation tube according to claim 1, wherein the flexible liquid storage bag (5) is a transparent bag made of a flexible material.
10. A method for separating blood cells using the blood cell separation tube according to claim 1, comprising the steps of:
s1, collecting fresh blood, placing the fresh blood into an anticoagulation bottle (1) filled with glass beads, adding the blood anticoagulation agent, and shaking the bottle to obtain anticoagulation;
s2, transferring the anticoagulated blood into a flexible liquid storage bag (5) filled with layering liquid through a connector, centrifuging to obtain the layered blood cell solution, and completing the separation of blood cells.
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| CN201710686598.8A CN107475094B (en) | 2017-08-11 | 2017-08-11 | Blood cell separation tube and separation method |
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Effective date of registration: 20230825 Address after: E5-A29, Luoyang Robot Intelligent Equipment Industrial Park, No. 96 Binhe North Road, Luoyang Area (High-tech), China (Henan) Pilot Free Trade Zone, Luoyang City, Henan Province 471000 Patentee after: Luoyang Jiaze Medical Technology Co.,Ltd. Address before: 471003 Jinghua Road, Jianxi District, Luoyang, Henan 24 Patentee before: THE FIRST AFFILIATED HOSPITAL OF HENAN University OF SCIENCE AND TECHNOLOGY |