CN107466847A - A kind of breeding method of winter resistance gumbo - Google Patents
A kind of breeding method of winter resistance gumbo Download PDFInfo
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- CN107466847A CN107466847A CN201710735405.3A CN201710735405A CN107466847A CN 107466847 A CN107466847 A CN 107466847A CN 201710735405 A CN201710735405 A CN 201710735405A CN 107466847 A CN107466847 A CN 107466847A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
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Abstract
The invention discloses a kind of breeding method of winter resistance gumbo, it is characterised in that including following aspect：（1）Seed storage, gumbo seed is handled using Gibberellins solution, mixes and is stored with culture matrix, and colchicine solution is heated at a temperature of 24 DEG C, stored；（2）Mutation breeding, atomization process first is carried out using active selenium solution to seed, ultraviolet light is reused and is irradiated in the case of alternating temperature, finally irradiated using gamma-rays；（3）Vernalization, mutagenesis seed is immersed constant temperature vernalization is carried out in Gibberellins solution；（4）Breeding, the breeding culture at a temperature of 35 DEG C, is screened to cold resistance seed；（5）Crossbreeding, mutagenesis seed are hybridized three times to gumbo seed, three generations's seed, four generation seeds, are obtained five generation seeds and are carried out scale plantation.
Description
Technical field
The invention belongs to growing vegetables technical field, and in particular to a kind of breeding method of winter resistance gumbo.
Background technology
Gumbo is annual herb plant, also known as okra, Abelmoschus esculentus, swordweed, is divided into green and red two kinds, its
The tender and crisp succulence of Fruit pod, it is lubricious it is oiliness, fragrance is unique, have extensive kind in Europe, Asia, the Middle East and Central-South subregion at present
Plant；Its habit likes temperature climate, can not resist cold, with deep fertile, sun-drenched soil.In gumbo containing abundant protein,
The nutritional ingredients such as fat, carbohydrate, vitamin, mineral matter, long-term consumption can improve body immunity, protection stomach lining, and
And there is certain curative effect to abscess of throat, dribbling urination and diabetes, there is preferable eating mouth feel and health value.But the autumn
The cold resistance of certain herbaceous plants with big flowers is poor, and its growth temperature adapted to is high, although its Seeded growth cycle is grown, is influenceed its cultivation by growth temperature
Kind time and scope are all limited by a relatively large margin；Because winter resistance is relevant with the heredity of gumbo, generally use mutagenesis nursery
With hybridizing method breeding, and use chemical reagent mutagenesis that there is stronger teratogenesis, toxic action, gained plantation mutation probability is low,
And seed mortality is caused, survival rate is low；Irradiation mutagenesis method mutation rate is high, but the direction that makes a variation is not known, occur compared with
More bad inhereditary feature is, it is necessary to carry out a large amount of screening operations；And hybridizing method, the inhereditary feature influence on gumbo are limited, and
The inhereditary feature obtained is easily lost.
The content of the invention
The present invention is directed to the problem of existing：Planting range, the cold resistance of gumbo is poor, and its growth temperature adapted to is high, though
Right its Seeded growth cycle length, but influenceed that it plants time and scope is all limited by a relatively large margin by growth temperature；Breeding
Method, generally use mutagenesis nursery and hybridizing method breeding, and use chemical reagent mutagenesis that there is stronger teratogenesis, poison and make
With gained plantation mutation probability is low, and causes seed mortality, and survival rate is low；Irradiation mutagenesis method mutation rate is high, still
Variation direction is not known, and more bad inhereditary feature occurs, it is necessary to carry out a large amount of screening operations；And hybridizing method, to gumbo
Inhereditary feature influence is limited, and the inhereditary feature obtained is easily lost.To solve the above problems, the invention provides one kind
The breeding method of winter resistance gumbo.
The present invention is achieved by the following technical solutions：
A kind of breeding method of winter resistance gumbo, comprises the following steps：
（1）Seed storage：5-7min is impregnated by being immersed after gumbo seed progress detoxification treatment in Gibberellins solution, improves gumbo kind
Careful cytoactive, then gumbo seed is mixed with culture matrix, it is put into hermetically sealed can and first stores 8- under 8-12 DEG C of constant temperature
10 days, the composition such as nutrition, plant hormone, can promote to grow in neutron absorption culture matrix, immunising seed power be improved, to culture medium
Colchicine solution is added in matter, is then stored 14-17 days under 2-4 DEG C of constant temperature, colchicine can induce seed low
Winter resistance variation is produced under the conditions of temperature, storage seed is made；
（2）Mutation breeding：Storage seed is first subjected to activation process, is put into spray chamber and is carried out using active selenium solution at atomization
Manage, the resistivity to irradiation, heavy metal can be improved after neutron absorption activity selenium, survival rate after mutagenesis is improved, by atomization process
Gumbo seed afterwards, which is placed in vacuum tank, carries out mutagenic treatment, and it is 0.1-0.14% to keep carbon dioxide volume content in vacuum tank,
Stimulate photosynthesis, improve plumule activity, vacuum tank temperature is first set to 20-24 DEG C, keep 30-40min, according to 0.5 DEG C/
Min speed is down to 6-8 DEG C, ultraviolet lighting intensity 210-240lux, temperature then is down into 3-5 DEG C with 0.2 DEG C/min speed, purple
Outer intensity of illumination 340-380lux, circulation carry out 6-8 cooling, heating, in low-temperature space by reducing heating and cooling rate, carried
High ultraviolet light irradiation intensity, variation probability of the seed in low-temperature space is improved, the mutation of winter resistance variability is produced, finally in temperature 3-
Using the gamma-rays irradiation 12-14min of 230-250 flower bud nurses at a temperature of 5 DEG C, the gamma-rays irradiation of low irradiance, seed can be improved
Winter resistance is mutated probability, and keeps the activity of seed, and mutagenesis seed is made；
（3）Vernalization：Mutagenesis seed is immersed constant temperature vernalization is carried out in Gibberellins solution, vernalization seed is made；
（4）Breeding：According to loess and culture matrix quality proportioning 4-6:1 laying seedbed, vernalization seed is sowed, treats seedling
It is long to be cultivated 7-10 days to slow cooling during 5-6cm height to 3-5 DEG C, choose cold-resistant seedling and carry out transplanting culture, obtain two generation seeds；
（5）Crossbreeding：Gumbo two generations seed is hybridized with gumbo seed, obtains three generations's seed, then by three generations's seed and two
Hybridized for seed, obtain four generation seeds, and hybridized using four generation seeds and two generation seeds, obtain five generation seeds, then will
Five generation seeds carry out large-scale planting；Transformation is hybridized by mutagenesis seed three times to gumbo seed, three generations's seed, four generation seeds,
Gumbo seed is obtained the The Character of Cold-tolerance of mutagenesis seed, and yield, quality, the speed of growth will not be had an impact, seed is lost
Transmissibility shape is stable.
Step（1）Described detoxification treatment, its detoxifying agent are liquor natrii hypochloritis, mass concentration 60ppm, the detoxification time
For 15-20min；Described Gibberellins solution, its mass concentration are 0.5%-0.7%；Described colchicum solution, its addition are
The 7%-9% of culture matrix quality, mass concentration 2%-3%.
Step（1）Described culture matrix, its compound method are：
Wood chip 24-29 parts, lichens 13-17 parts, peat 9-13 parts, kudzu-vine root powder 8-10 parts, pawpaw skin 5-7 are weighed according to quality meter part
Part, nandina 4-6 parts, beet tops 6-8 parts, marine alga 3-6 parts, durian peel 4-6 parts, preparation raw material is added after dried, crushed
Its gross mass 4%-5% EM bacteria agent, culture matrix is made after fermentation；Wherein gumbo seed and culture matrix quality proportioning are 1:6-
8。
Step（2）Described activation process, its temperature are 18-23 DEG C, humidity 60%-65%, time 3-4h.
Step（2）Described atomization process, its temperature are 35-37 DEG C, pressure 0.2-0.4MPa, nebulisation time 35-
40min；Described active selenium solution, its mass concentration are 1%-2%.
Step（3）Described constant temperature vernalization, 23-27 DEG C of its temperature, time 8-10h.
The present invention has advantages below compared with prior art：Seed storage method, first gumbo seed is entered using gibberellin
Row processing, seed cell activity is improved, is stored at a temperature of being blended in 8-12 DEG C with culture matrix, abundant battalion is contained in fermentation substrate
Form point, bactericidal composition and auxin class, absorption of the gumbo seed to culture matrix composition can be improved, raising is exempted from
Epidemic disease power and the speed of growth；Stored into culture matrix after addition colchicine at a temperature of 2-4 DEG C, pass through colchicine mutagenesis gumbo
Seed resistance heredity at low temperature, induction seed produce the individual with The Character of Cold-tolerance.Mutation breeding, it is first molten using active selenium
Liquid carries out atomization process to seed, and self-protection energy under the strong stimulations such as irradiation, metal can be improved after neutron absorption activity selenium solution
Power, the survival rate of mutagenesis seed is improved, improve gas concentration lwevel in vacuum tank, stimulate seed photosynthesis, improve seed embryo
Bud vigor；And becoming middle benefit gas by stimulation of the ultraviolet light to gumbo seed, seed can be made to produce gene mutation, and in low-temperature space
Temperature decline and the rate of climb are reduced, increases variation probability of the seed in low-temperature space by improving UV Light intensity, produces
Variation gumbo seed with stronger anti-seismic design；Because ultraviolet light induced mutation effect is limited, seed mutation probability is smaller, and
Seed under low temperature is radiated using low irradiation gamma-rays, seed winter resistance mutation probability can be effectively improved, and keep
The activity of seed.Crossbreeding, winter resistance gumbo seed is obtained by mutation breeding, although its winter resistance is stronger, its
Other inhereditary features may change, it may appear that the speed of growth is slow, the phenomenon that yields poorly, causes seed obtained by mutagenesis to lose cultivation
Train meaning；And by mutagenesis seed gumbo seed, three generations's seed, four generation seeds are hybridized with transformation three times, obtain gumbo seed
The The Character of Cold-tolerance of mutagenesis seed, and yield, quality, the speed of growth will not be had an impact, seed stabilization characteristics of genetics will not
Produce Loss.
Embodiment
Embodiment 1：
A kind of breeding method of winter resistance gumbo, comprises the following steps：
（1）Seed storage：5.5min is impregnated by being immersed after gumbo seed progress detoxification treatment in Gibberellins solution, improves gumbo kind
Careful cytoactive, then gumbo seed is mixed with culture matrix, it is transferred in hermetically sealed can and is first stored 9 days under 9 DEG C of constant temperatures, is planted
Son absorbs the composition such as nutrition, plant hormone in culture matrix, can promote to grow, and improves immunising seed power, adds into culture matrix
Enter colchicine solution, then stored 15 days under 2.5 DEG C of constant temperatures, colchicine can induce seed and produce under cryogenic
Raw winter resistance variation, is made storage seed；
（2）Mutation breeding：Storage seed is first subjected to activation process, is put into spray chamber and is carried out using active selenium solution at atomization
Manage, the resistivity to irradiation, heavy metal can be improved after neutron absorption activity selenium, survival rate after mutagenesis is improved, by atomization process
Gumbo seed afterwards, which is placed in vacuum tank, carries out mutagenic treatment, and it is 0.12% to keep carbon dioxide volume content in vacuum tank, is stimulated
Photosynthesis, plumule activity is improved, vacuum tank temperature is first set to 21 DEG C, 33min is kept, is down to according to 0.5 DEG C/min speed
6.5 DEG C, ultraviolet lighting intensity 220lux, temperature is then down to 3.5 DEG C with 0.2 DEG C/min speed, ultraviolet lighting intensity
350lux, circulation carry out 7 coolings, heating, and in low-temperature space by reducing heating and cooling rate, it is strong to improve ultraviolet light irradiation
Degree, variation probability of the seed in low-temperature space is improved, produce the mutation of winter resistance variability, finally used at a temperature of 3.5 DEG C of temperature
The gamma-rays irradiation 13min of 235 flower bud nurses, the gamma-rays irradiation of low irradiance, can improve seed winter resistance mutation probability, and protect
The activity of seed is held, mutagenesis seed is made；
（3）Vernalization：Mutagenesis seed is immersed constant temperature vernalization is carried out in Gibberellins solution, vernalization seed is made；
（4）Breeding：According to loess and culture matrix quality proportioning 4:1 laying seedbed, vernalization seed is sowed, and treats that seedling grows
Cultivated 8 days to slow cooling during 5-6cm height to 3.5 DEG C, choose cold-resistant seedling and carry out transplanting culture, obtain two generation seeds；
（5）Crossbreeding：Gumbo two generations seed is hybridized with gumbo seed, obtains three generations's seed, then by three generations's seed and two
Hybridized for seed, obtain four generation seeds, and hybridized using four generation seeds and two generation seeds, obtain five generation seeds, then will
Five generation seeds carry out large-scale planting；Transformation is hybridized by mutagenesis seed three times to gumbo seed, three generations's seed, four generation seeds,
Gumbo seed is obtained the The Character of Cold-tolerance of mutagenesis seed, and yield, quality, the speed of growth will not be had an impact, seed is lost
Transmissibility shape is stable.
Step（1）Described detoxification treatment, its detoxifying agent are liquor natrii hypochloritis, mass concentration 60ppm, the detoxification time
For 16min；Described Gibberellins solution, its mass concentration are 0.55%；Described colchicum solution, its addition are culture medium
The 7.5% of matter quality, mass concentration 2.3%.
Step（1）Described culture matrix, its compound method are：
25 parts of wood chip, 14 parts of lichens, 10 parts of peat, 8.5 parts of kudzu-vine root powder, 5.6 parts of pawpaw skin, nandina are weighed according to quality meter part
4.7 parts, 6.4 parts of beet tops, 4 parts of marine alga, 4.8 parts of durian peel, preparation raw material is added to its gross mass 4.2% after dried, crushed
EM bacteria agent, after fermentation be made culture matrix；Wherein gumbo seed and culture matrix quality proportioning are 1:7.
Step（2）Described activation process, its temperature are 19 DEG C, humidity 62%, time 3.5h.
Step（2）Described atomization process, its temperature are 36 DEG C, pressure 0.25MPa, nebulisation time 36min；Described work
Property selenium solution, its mass concentration be 1.4%.
Step（3）Described constant temperature vernalization, 24 DEG C of its temperature, time 8.5h.
Embodiment 2：
Compared with embodiment 1, step changes in the following areas the present embodiment 2：
Step（1）Described detoxification treatment, its detoxifying agent are liquor natrii hypochloritis, mass concentration 60ppm, and the detoxification time is
18min；Described Gibberellins solution, its mass concentration are 0.68%；Described colchicum solution, its addition are culture matrix
The 8.4% of quality, mass concentration 2.7%.
Step（1）Seed storage, its method are：
Gumbo seed is mixed with culture matrix, is transferred in hermetically sealed can and is first stored 9.5 days under 11 DEG C of constant temperatures, Ran Hou
Stored 16 days under 3.5 DEG C of constant temperatures.
Step（1）Described culture matrix, its compound method are：
28 parts of wood chip, 16 parts of lichens, 12 parts of peat, 9.6 parts of kudzu-vine root powder, 6.7 parts of pawpaw skin, nandina are weighed according to quality meter part
5.8 parts, 7.2 parts of beet tops, 5 parts of marine alga, 5.4 parts of durian peel, preparation raw material is added to its gross mass 4.8% after dried, crushed
EM bacteria agent, after fermentation be made culture matrix；Wherein gumbo seed and culture matrix quality proportioning are 1:8.
Step（2）Described activation process, its temperature are 21 DEG C, humidity 64%, time 4h.
Step（2）Described atomization process, its temperature are 36 DEG C, pressure 0.35MPa, nebulisation time 38min；Described work
Property selenium solution, its mass concentration be 1.7%.
Step（2）Mutation breeding, its method are：
Carbon dioxide volume content is 0.13% in vacuum tank, and vacuum tank temperature first is set into 23 DEG C, 37min is kept, according to 0.5
DEG C/min speed is down to 7.5 DEG C, ultraviolet lighting intensity 235lux, and temperature then is down into 4.5 DEG C with 0.2 DEG C/min speed, it is purple
Outer intensity of illumination 370lux, circulation are carried out 8 coolings, heating, finally penetrated at a temperature of 4.5 DEG C of temperature using the γ of 240 flower bud nurses
Line irradiates 14min.
Step（3）Described constant temperature vernalization, 26 DEG C of its temperature, time 9.5h.
Contrast 1：
This contrast 1 does not carry out step compared with embodiment 1（1）Seed storage, other steps are same as Example 1.
Contrast 2：
This contrast 2 does not carry out step compared with embodiment 1（2）Middle intermittent warming, other steps are same as Example 1.
Contrast 3：
This contrast 3 does not carry out step compared with embodiment 1（2）Middle gamma-rays irradiation, other steps are same as Example 1.
Contrast 4：
This contrast 4 does not carry out step compared with embodiment 2（4）Middle low temperature breeding, other steps are same as Example 2.
Contrast 5：
This contrast 5 does not carry out step compared with embodiment 2（5）Crossbreeding, other steps are same as Example 2.
Control group：
Control group is using gumbo seed as reference；And induction variation is carried out using chemical inducer, observe it and induce variation situation.
To embodiment 1, embodiment 2, contrast 1, contrast 2, contrast 3, contrast 4, contrast 5 and control group experimental program, statistics
Every per mu yield, the speed of growth of gumbo（1 month growing height）, cold resistance mutagenesis probability, minimum cold-resistant temperature.
Experimental data：
| Project | Per per mu yield kg | Speed of growth cm | Cold resistance mutagenesis probability % | Minimum cold-resistant temperature DEG C |
| Embodiment 1 | 1476 | 5.4 | 9.87% | 3 |
| Embodiment 2 | 1468 | 5.3 | 9.79% | 3 |
| Contrast 1 | / | / | 8.24% | 4 |
| Contrast 2 | / | / | 6.52% | 3 |
| Contrast 3 | / | / | 7.70% | 5 |
| Contrast 4 | 1324 | 4.3 | 7.88% | / |
| Contrast 5 | 1217 | 3.9 | 9.74% | / |
| Control group | 1392 | 5.3 | 2.35% | 8 |
Synthesis result：Gumbo obtained by the inventive method, and control group compare, its yield and the speed of growth and the previous cause that do not make a variation,
And cold resistance mutagenesis probability is higher by 7.52% compared with chemical inducer, minimum cold-resistant temperature is low 6 DEG C.At seed storage and alternating temperature
Reason method, it is 1.63%, 3.35% that can improve seed cold resistance mutagenesis probability, and the temperature that resists cold reduces by 1 DEG C, 3 DEG C；And use gamma-rays
Irradiation, its cold resistance mutagenesis probability improve 2.17%, and the temperature that resists cold declines 2 DEG C；By breeding and crossbreeding, seed can be made to exist
While obtaining cold resistance hereditary feature, yield and the speed of growth is kept to be consistent with former gumbo.
Claims (6)
1. a kind of breeding method of winter resistance gumbo, it is characterised in that comprise the following steps：
（1）Seed storage：Gumbo seed immerse in Gibberellins solution after detoxification treatment to impregnate 5-7min, then by gumbo kind
Son mixes with culture matrix, is put into hermetically sealed can and is first stored 8-10 days under 8-12 DEG C of constant temperature, is added into culture matrix
Colchicine solution, then stored 14-17 days under 2-4 DEG C of constant temperature, storage seed is made；
（2）Mutation breeding：Storage seed is first subjected to activation process, is put into spray chamber and is carried out using active selenium solution at atomization
Reason, the gumbo seed after atomization process is placed in vacuum tank and carries out mutagenic treatment, keeps carbon dioxide volume in vacuum tank to contain
Measure as 0.1-0.14%, vacuum tank temperature is first set to 20-24 DEG C, keep 30-40min, 6-8 is down to according to 0.5 DEG C/min speed
DEG C, ultraviolet lighting intensity 210-240lux, temperature is then down to 3-5 DEG C with 0.2 DEG C/min speed, ultraviolet lighting intensity 340-
380lux, circulation carry out 6-8 cooling, heating, and the gamma-rays photograph of 230-250 flower bud nurses is finally used at a temperature of 3-5 DEG C of temperature
12-14min is penetrated, mutagenesis seed is made；
（3）Vernalization：Mutagenesis seed is immersed constant temperature vernalization is carried out in Gibberellins solution, vernalization seed is made；
（4）Breeding：According to loess and culture matrix quality proportioning 4-6:1 laying seedbed, vernalization seed is sowed, treats seedling
It is long to be cultivated 7-10 days to slow cooling during 5-6cm height to 3-5 DEG C, choose cold-resistant seedling and carry out transplanting culture, obtain two generation seeds；
（5）Crossbreeding：Gumbo two generations seed is hybridized with gumbo seed, obtains three generations's seed, then by three generations's seed and two
Hybridized for seed, obtain four generation seeds, and hybridized using four generation seeds and two generation seeds, obtain five generation seeds, then will
Five generation seeds carry out large-scale planting.
2. the breeding method of winter resistance gumbo as claimed in claim 1, it is characterised in that step（1）Described detoxification treatment, its
Detoxifying agent is liquor natrii hypochloritis, and mass concentration 60ppm, the detoxification time is 15-20min；Described Gibberellins solution, its matter
Amount concentration is 0.5%-0.7%；Described colchicum solution, its addition are the 7%-9% of culture matrix quality, and mass concentration is
2%-3%。
3. the breeding method of winter resistance gumbo as claimed in claim 1, it is characterised in that step（1）Described culture matrix, its
Compound method is：
Wood chip 24-29 parts, lichens 13-17 parts, peat 9-13 parts, kudzu-vine root powder 8-10 parts, pawpaw skin 5-7 are weighed according to quality meter part
Part, nandina 4-6 parts, beet tops 6-8 parts, marine alga 3-6 parts, durian peel 4-6 parts, preparation raw material is added after dried, crushed
Its gross mass 4%-5% EM bacteria agent, culture matrix is made after fermentation；Wherein gumbo seed and culture matrix quality proportioning are 1:6-
8。
4. the breeding method of winter resistance gumbo as claimed in claim 1, it is characterised in that step（2）Described activation process, its
Temperature is 18-23 DEG C, humidity 60%-65%, time 3-4h.
5. the breeding method of winter resistance gumbo as claimed in claim 1, it is characterised in that step（2）Described atomization process, its
Temperature is 35-37 DEG C, pressure 0.2-0.4MPa, nebulisation time 35-40min；Described active selenium solution, its mass concentration are
1%-2%。
6. the breeding method of winter resistance gumbo as claimed in claim 1, it is characterised in that step（3）Described constant temperature vernalization, its
23-27 DEG C of temperature, time 8-10h.
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| CN201710735405.3A CN107466847A (en) | 2017-08-24 | 2017-08-24 | A kind of breeding method of winter resistance gumbo |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114651658A (en) * | 2022-04-11 | 2022-06-24 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Creation and identification method of cold-resistant seeds of macadimia nuts |
-
2017
- 2017-08-24 CN CN201710735405.3A patent/CN107466847A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114651658A (en) * | 2022-04-11 | 2022-06-24 | 广西壮族自治区亚热带作物研究所（广西亚热带农产品加工研究所） | Creation and identification method of cold-resistant seeds of macadimia nuts |
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| WD01 | Invention patent application deemed withdrawn after publication | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20171215 |