CN107466756B - early identification and treatment method for rice cold damage - Google Patents
early identification and treatment method for rice cold damage Download PDFInfo
- Publication number
- CN107466756B CN107466756B CN201710762082.7A CN201710762082A CN107466756B CN 107466756 B CN107466756 B CN 107466756B CN 201710762082 A CN201710762082 A CN 201710762082A CN 107466756 B CN107466756 B CN 107466756B
- Authority
- CN
- China
- Prior art keywords
- rice
- leaves
- damage
- content
- malondialdehyde
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/20—Cereals
- A01G22/22—Rice
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B15/00—Organic phosphatic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Physics & Mathematics (AREA)
- General Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Health & Medical Sciences (AREA)
- Environmental Sciences (AREA)
- General Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Botany (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Catching Or Destruction (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a rice cold damage early identification and a treatment method thereof, wherein the rice cold damage early identification and the treatment method thereof are characterized in that the rice cold damage is graded according to different malonaldehyde contents and is treated according to different cold damage grades, and in the case of slight damage, ATP aqueous solution is sprayed on leaves; spraying ATP and alkaloid water solution on the leaves with moderate harm; and (3) heavily damaging, namely spraying ATP, alkaloid and sodium chloride solution on the leaves, wherein the ATP mainly supplements nutrition required by plant growth, and the alkaloid and the sodium chloride only have an inhibiting effect on malondialdehyde so as to reduce the damage of the malondialdehyde to plant organisms.
Description
Technical Field
the invention relates to the technical field of rice planting, in particular to an early identification and treatment method for rice cold injury.
Background
the cold damage of rice at low temperature generally means that the rice is affected by the low temperature below the lowest critical temperature of growth, so that the rice cannot grow normally and the yield is reduced. The cold damage at low temperature is one of the main obstacles for the production of rice in cold regions. This generally occurs in two phases: the first is the freeze injury in the seedling raising period of 4-5 months, i.e. the seedlings are withered. And bald tip and shrivelled grains caused by delayed cold damage and barrier cold damage in the field, even no ear-picking and the like.
Symptoms and signs
After the seedling stage is damaged by low temperature, the leaf color of the whole plant turns yellow, yellow leaves are generated at the lower part of the plant, some leaves are brown, and part of the leaves are white or yellow to yellowish white transverse streak spots, which are commonly called as 'yellow nodulation' or 'white nodulation'. The daily average temperature is continuously lower than 12 ℃ in the seedling stage of 2-3 leaves, and the seedling rot is easy to generate. The cold damage in the booting stage reduces the glumous flower number, and the development of young ears is inhibited. The cold damage in the flowering period often causes sterility, i.e. fertilization failure. The low-temperature normally-opened flowering phase is delayed, the maturation phase is delayed, and poor maturation is caused. The elongation of cold damage grains in the mature period becomes slow, when the cold damage grains are frosted, the mature process stops, the dry grain weight is reduced, and the large-area yield reduction of the rice is caused.
disease and disease
the rice has obstacle type cold damage, the ear is a low-temperature sensitive part, the anther is an organ which directly senses low temperature and affects fructification, and the reaction to the low temperature is different on one ear. The sensitive period of the obstacle type cold injury is the initial stage of microspore formation, and the meiotic period is only about 1 day away from the initial stage of microspore formation in the view of the rice growth process. The critical temperatures for short-term cryogenic treatment resulting in a sterile booting stage in production were the following 3 cases: the initial temperature of sperm-free is 18-20 ℃; the dangerous temperature of the obstacle type cold damage is 15-17 ℃; the critical temperature for complete spermiation is 10-12 ℃. In the field, day temperature is high, night temperature is low, the failure rate caused by day and night temperature change and stable low temperature is different, and when the day temperature is high enough to compensate the low temperature at night, fructification cannot be influenced. In the process of rice growth, when the rice is subjected to low-temperature conditions below a proper temperature, photosynthesis and respiration are inhibited, and substance metabolism and energy metabolism are abnormal. The test proves that: the low temperature affects the net assimilation rate, the rhizosphere temperature is lower than 16 ℃, and the net assimilation rate is reduced. In a new cold damage mechanism, after the phase change of the biological membrane is caused by low temperature, the biological membrane is damaged, so that the metabolic system is disordered and the damage is caused. Experts believe that the low temperature causes the phase change of the biofilm of the temperature-favored crops, which is the initial reaction of the rice to the subzero low temperature and is also the initial cause of the cold injury. The lipid material of the membrane is solidified, and the membrane material is changed into a gel state from a liquid crystal state. The hydrocarbon chain of fatty acid in the membrane is changed from disordered arrangement to ordered arrangement, so that the thickness and the appearance of the biological membrane are changed, and the membrane surface generates pore channels and cracks due to the contraction of the membrane. The permeability of the biological membrane is increased, so that human solutes in cells are infiltrated, the respiration is weakened, and toxic substances such as acetaldehyde, ethanol and the like are accumulated in anaerobic respiration in the cells, so that the cells are damaged, and the growth of crops is inhibited. The process is reversible, with short duration of low temperature or not strong. After the temperature rises back to normal, the film phase is converted to liquid crystal state again, and the metabolism of the crops is recovered to normal. If the intensity of low temperature is large and the duration is long, cell membranes are degraded into an irreversible state, and cells and tissues die. Causing the plant to wilt or die.
the northeast rice growing region, the northwest rice growing region, the Chinese rice growing region, the south China rice growing region and the southwest rice growing region in China are located at geographical positions of 31 latitudes across the north and south, the height difference is 2700m, the growing seasons are greatly different in length, and ecological conditions such as light, temperature, water and the like are different, so that respective rice growing systems, variety types and planting modes are formed, and the occurrence frequency, types and damage degrees of cold damage in various regions are different.
according to the characteristics of the rice in the northeast region, various cold damage identification methods are summarized, the most appropriate cold damage early-onset identification method is selected, and the treatment is carried out according to different disease degrees, so that the damage of the cold damage to the rice can be reduced to the greatest extent.
disclosure of Invention
Based on the technical problems in the background art, the invention provides a method for early identification and treatment of rice cold injury.
The technical scheme of the invention is as follows:
a rice cold injury early identification and treatment method comprises the following steps:
A. In the stage of one leaf and one heart in the three-leaf stage, collecting the leaves of seedlings to carry out Malondialdehyde (MDA) detection to obtain the malondialdehyde content in unit fresh weight;
B. Synchronously adopting a greenhouse cultivation mode to cultivate rice as a comparative example;
C. Grading the cold damage of the rice according to different malonaldehyde content increasing ratios;
D. aiming at different rice cold injury grades, different treatment methods are respectively adopted to treat rice leaves and soil.
Preferably, in the step a, the method for detecting malondialdehyde comprises the following steps:
the method comprises the following steps: taking 0.5g of rice leaves, adding 5ml of 5% TCA, and centrifuging the homogenate obtained after grinding for 10min at 3000 r/min;
step two: taking 2ml of supernatant, adding 2ml of 0.67% TBA, mixing, boiling in water bath at 100 ℃ for 30min, cooling, and centrifuging again;
Step three: and respectively measuring the absorbances of the supernatant at 450mn, 532nm and 600nm, calculating the MDA concentration according to a formula, and calculating the MDA content (mu mol/g) in unit fresh weight.
the formula is as follows:
A-A＝155000×C×L (1)
C(μ mol/g)＝6.45(A-A)-0.56A (2)
In the formula, A450, A532 and A600 respectively represent absorbance values under the wavelengths of 450mn, 532nm and 600nm, the concentration of MDA in the rice leaf sample extracting solution can be directly obtained by using the formula (2), and the content of MDA in the rice leaf can be further calculated.
Preferably, in the step C, grading the cold damage of the rice according to different malonaldehyde contents; the content of the malonaldehyde is increased by less than 50 percent, and the malonaldehyde is considered not to be damaged; increasing the malondialdehyde content by 50-80% (excluding 80%) and considering the damage as slight; increasing the malondialdehyde content by 80-150% (not including 150%) and considering the damage as moderate; the malondialdehyde content was increased by more than 150% and considered severely compromised.
according to research, after the rice is subjected to cold injury treatment, the content of malondialdehyde is improved to different degrees, the degree of the increase is most obvious in the stage of one leaf and one heart in the three-leaf period, and the content of the cold-resistant variety is improved by more than 50%.
preferably, in the step D, the ATP aqueous solution is sprayed on the leaves with slight damage.
preferably, in the step D, the ATP and the aqueous solution of the alkaloid are sprayed on the leaves for medium harm.
preferably, in the step D, the leaves are sprayed with ATP, alkaloid and sodium chloride solution to achieve serious harm.
according to the early identification and treatment method of the cold damage of the rice, the cold damage of the rice is graded according to different malonaldehyde contents, and the cold damage of the rice is treated according to different cold damage grades, wherein the leaves are sprayed with ATP aqueous solution when the cold damage is slight; spraying ATP and alkaloid water solution on the leaves with moderate harm; and (3) heavily damaging, namely spraying ATP, alkaloid and sodium chloride solution on the leaves, wherein the ATP mainly supplements nutrition required by plant growth, and the alkaloid and the sodium chloride only have an inhibiting effect on malondialdehyde so as to reduce the damage of the malondialdehyde to plant organisms.
Detailed Description
Taking the rice floral scent No. 2 as an example, specific experiments are made on the early identification of the cold injury of rice and the treatment method thereof, and the following examples are obtained.
Example 1:
a rice cold injury early identification and treatment method comprises the following steps:
A. In the stage of one leaf and one heart in the three-leaf stage, collecting the leaves of seedlings to carry out Malondialdehyde (MDA) detection to obtain the malondialdehyde content in unit fresh weight;
B. Synchronously adopting a greenhouse cultivation mode to cultivate rice as a comparative example;
C. Grading the cold damage of the rice according to different malonaldehyde content increasing ratios;
D. Aiming at different rice cold injury grades, different treatment methods are respectively adopted to treat rice leaves and soil.
in the step a, the method for detecting malondialdehyde comprises the following steps:
the method comprises the following steps: taking 0.5g of rice leaves, adding 5ml of 5% TCA, and centrifuging the homogenate obtained after grinding for 10min at 3000 r/min;
Step two: taking 2ml of supernatant, adding 2ml of 0.67% TBA, mixing, boiling in water bath at 100 ℃ for 30min, cooling, and centrifuging again;
Step three: and respectively measuring the absorbances of the supernatant at 450mn, 532nm and 600nm, calculating the MDA concentration according to a formula, and calculating the MDA content (mu mol/g) in unit fresh weight.
the formula is as follows:
A-A＝155000×C×L (1)
C(μ mol/g)＝6.45(A-A)-0.56A (2)
in the formula, A450, A532 and A600 respectively represent absorbance values under the wavelengths of 450mn, 532nm and 600nm, the concentration of MDA in the rice leaf sample extracting solution can be directly obtained by using the formula (2), and the content of MDA in the rice leaf can be further calculated.
In the step C, grading the cold damage of the rice according to different malonaldehyde contents; the malondialdehyde content was increased by 76%, which was considered a mild hazard.
spraying ATP aqueous solution on the leaves.
the detection shows that the yield of the untreated rice is reduced by 30-45%, and the yield of the treated rice is reduced by 3-8%.
Example 2:
a rice cold injury early identification and treatment method comprises the following steps:
A. In the stage of one leaf and one heart in the three-leaf stage, collecting the leaves of seedlings to carry out Malondialdehyde (MDA) detection to obtain the malondialdehyde content in unit fresh weight;
B. synchronously adopting a greenhouse cultivation mode to cultivate rice as a comparative example;
C. Grading the cold damage of the rice according to different malonaldehyde content increasing ratios;
D. aiming at different rice cold injury grades, different treatment methods are respectively adopted to treat rice leaves and soil.
In the step a, the method for detecting malondialdehyde comprises the following steps:
The method comprises the following steps: taking 0.5g of rice leaves, adding 5ml of 5% TCA, and centrifuging the homogenate obtained after grinding for 10min at 3000 r/min;
step two: taking 2ml of supernatant, adding 2ml of 0.67% TBA, mixing, boiling in water bath at 100 ℃ for 30min, cooling, and centrifuging again;
Step three: and respectively measuring the absorbances of the supernatant at 450mn, 532nm and 600nm, calculating the MDA concentration according to a formula, and calculating the MDA content (mu mol/g) in unit fresh weight.
The formula is as follows:
A-A＝155000×C×L (1)
C(μ mol/g)＝6.45(A-A)-0.56A (2)
In the formula, A450, A532 and A600 respectively represent absorbance values under the wavelengths of 450mn, 532nm and 600nm, the concentration of MDA in the rice leaf sample extracting solution can be directly obtained by using the formula (2), and the content of MDA in the rice leaf can be further calculated.
In the step C, grading the cold damage of the rice according to different malonaldehyde contents; the malondialdehyde content increased by 124%, which is considered a moderate hazard.
spraying ATP and alkaloid water solution on the leaves.
Through detection, the yield of the untreated rice is reduced by 60-70%, and the yield of the treated rice is reduced by 15-25%.
example 3:
A rice cold injury early identification and treatment method comprises the following steps:
A. In the stage of one leaf and one heart in the three-leaf stage, collecting the leaves of seedlings to carry out Malondialdehyde (MDA) detection to obtain the malondialdehyde content in unit fresh weight;
B. Synchronously adopting a greenhouse cultivation mode to cultivate rice as a comparative example;
C. grading the cold damage of the rice according to different malonaldehyde content increasing ratios;
D. aiming at different rice cold injury grades, different treatment methods are respectively adopted to treat rice leaves and soil.
In the step a, the method for detecting malondialdehyde comprises the following steps:
The method comprises the following steps: taking 0.5g of rice leaves, adding 5ml of 5% TCA, and centrifuging the homogenate obtained after grinding for 10min at 3000 r/min;
Step two: taking 2ml of supernatant, adding 2ml of 0.67% TBA, mixing, boiling in water bath at 100 ℃ for 30min, cooling, and centrifuging again;
Step three: and respectively measuring the absorbances of the supernatant at 450mn, 532nm and 600nm, calculating the MDA concentration according to a formula, and calculating the MDA content (mu mol/g) in unit fresh weight.
The formula is as follows:
A-A＝155000×C×L (1)
C(μ mol/g)＝6.45(A-A)-0.56A (2)
In the formula, A450, A532 and A600 respectively represent absorbance values under the wavelengths of 450mn, 532nm and 600nm, the concentration of MDA in the rice leaf sample extracting solution can be directly obtained by using the formula (2), and the content of MDA in the rice leaf can be further calculated.
In the step C, grading the cold damage of the rice according to different malonaldehyde contents; the malondialdehyde content increased by more than 175% and was considered severely compromised.
Spraying ATP, alkaloid and sodium chloride solution on the leaves.
Through detection, the yield of the untreated rice is reduced by 85-90%, and the yield of the treated rice is reduced by 35-40%.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (4)
1. The early identification and treatment method for the cold damage of the rice is characterized by comprising the following steps of:
A. in the stage of one leaf and one heart in the three-leaf stage, collecting the leaves of seedlings to carry out Malondialdehyde (MDA) detection to obtain the malondialdehyde content in unit fresh weight;
B. synchronously adopting a greenhouse cultivation mode to cultivate rice as a comparative example;
C. grading the cold damage of the rice according to different malonaldehyde content increasing ratios;
D. aiming at different rice cold injury grades, respectively adopting different treatment methods to treat rice leaves and soil;
in the step a, the method for detecting malondialdehyde comprises the following steps:
The method comprises the following steps: taking 0.5g of rice leaves, adding 5% of TCA5ml, and centrifuging the homogenate obtained after grinding at 3000r/min for 10 min;
step two: collecting supernatant 2ml, adding 0.67% TBA2ml, mixing, boiling in 100 deg.C water bath for 30min, cooling, and centrifuging again;
Step three: respectively measuring the absorbances of the supernatant at 450mn, 532nm and 600nm, calculating the MDA concentration according to a formula, and calculating the MDA content (mu mol/g) in unit fresh weight;
the formula is as follows:
A-A＝155000×C×L (1)
C(μmol/g)＝6.45(A-A)-0.56A (2)
in the formula, A450, A532 and A600 respectively represent absorbance values under the wavelengths of 450mn, 532nm and 600nm, the concentration of MDA in the rice leaf sample extracting solution can be directly obtained by using the formula (2), and the content of MDA in the rice leaf can be further calculated;
in the step C, grading the cold damage of the rice according to different malonaldehyde contents; the content of the malonaldehyde is increased by less than 50 percent, and the malonaldehyde is considered not to be damaged; increasing the malondialdehyde content by 50-80%, and considering the result as slight harm; increasing the malondialdehyde content by 80-150%, and considering the increase as moderate harm; the malondialdehyde content was increased by more than 150% and considered severely compromised.
2. the method for early detection and treatment of rice chilling injury according to claim 1, wherein in step D, the leaves are sprayed with ATP aqueous solution for mild damage.
3. the method for early detection and treatment of rice cold injury as claimed in claim 1, wherein in step D, the moderate damage is achieved by spraying ATP and aqueous alkaloid solution on the leaves.
4. The method for early detection and treatment of rice chilling injury according to claim 1, wherein in step D, the leaf is sprayed with ATP, alkaloid and sodium chloride solution for severe damage.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710762082.7A CN107466756B (en) | 2017-08-30 | 2017-08-30 | early identification and treatment method for rice cold damage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710762082.7A CN107466756B (en) | 2017-08-30 | 2017-08-30 | early identification and treatment method for rice cold damage |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107466756A CN107466756A (en) | 2017-12-15 |
| CN107466756B true CN107466756B (en) | 2019-12-06 |
Family
ID=60603846
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710762082.7A Expired - Fee Related CN107466756B (en) | 2017-08-30 | 2017-08-30 | early identification and treatment method for rice cold damage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107466756B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109222192B (en) * | 2018-09-10 | 2021-07-13 | 云南省烟草农业科学研究院 | Flue-cured tobacco management and baking method for alleviating cold damage tobacco based on salicylic acid application |
| CN109781722A (en) * | 2019-03-28 | 2019-05-21 | 中国林业科学研究院林业研究所 | A kind of measuring method of Chinese catalpa leaf malondialdehyde content |
| CN111557304B (en) * | 2020-05-22 | 2021-12-03 | 中国农业科学院油料作物研究所 | Application of alkaloid atropine in relieving low-temperature freezing injury stress of plant seedlings |
| CN113917087B (en) * | 2021-10-19 | 2023-09-01 | 云南大学 | Method for monitoring occurrence and morbidity degree of plant diseases and application thereof |
| CN114804958A (en) * | 2022-05-25 | 2022-07-29 | 黑龙江八一农垦大学 | Novel rice seedbed seedling strengthening agent |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102435564B (en) * | 2011-09-19 | 2013-09-18 | 南京农业大学 | Method for estimating plant nitrogen content based on three-band spectral index |
| CN104418666A (en) * | 2013-09-03 | 2015-03-18 | 天津青野绿水水稻种植专业合作社 | Compound fertilizer for paddies and preparation method of compound fertilizer |
| KR20160102858A (en) * | 2015-02-23 | 2016-08-31 | 임영기 | BAEK SAN byeonongsa hwangsanaheon sibi jeungsan jaebaegisul |
| CN105557411A (en) * | 2015-12-15 | 2016-05-11 | 广西壮族自治区农业科学院水稻研究所 | Method for identifying heat-resistant seed setting performance of early rice at earing and flowering stages based on hour cumulative temperature |
-
2017
- 2017-08-30 CN CN201710762082.7A patent/CN107466756B/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN107466756A (en) | 2017-12-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107466756B (en) | early identification and treatment method for rice cold damage | |
| Yazdanpanah et al. | The interaction between drought stress and salicylic and ascorbic acids on some biochemical characteristics of Satureja hortensis | |
| CN102599040B (en) | Soilless blueberry cultivation method | |
| CN101253846B (en) | Passionflower tree type nutrient fluid cultivation method | |
| CN106613702B (en) | Method for relieving drought stress of kiwi fruit trees | |
| CN104054550A (en) | Viticulture method | |
| CN114946856A (en) | Application of 2-amino-3-hydroxy-3-methylbutyric acid in promoting plant growth | |
| Li et al. | Response of tomato plants to saline water as affected by carbon dioxide supplementation. I. Growth, yield and fruit quality | |
| CN111296264A (en) | Method for soilless culture of blackberries or blueberries under open-field condition | |
| Li | Growth and Development of Sugarcane (Saccharum spp. Hybrid) and Its Relationship with Environmental Factors | |
| Miyasaka et al. | Promising olive cultivars for oil production in Hawaii | |
| JP6789612B2 (en) | Growth improver for plants and method of manufacturing plants using it | |
| El-Saeed et al. | Changes the microclimate using some protection treatments for early grape production in south of Egypt | |
| Krasova | Parameters of apple tree variety resistant to winter unfavorable conditions | |
| Erez et al. | Container-grown peach orchards | |
| Karvonen | Northern Viticulture: Reviews and Studies | |
| CN107232200A (en) | Defend the composite regulator and method of wheat Spring cold injury | |
| Dwivedi | A scenario of abiotic stress impact on production potential of sugarcane: A climate resilient crop | |
| Jung et al. | Temperature changes under plastic film rain shelter using different concentration of shading paint in vineyard | |
| Gade et al. | Leaf reddening and its management in cotton | |
| CN106508569A (en) | Method for reducing red kiwifruit dropping | |
| CN106961993A (en) | Dragon fruit monofilm method of overwintering cultivation | |
| Cho et al. | Promotion of gold kiwifruit'Haegeum'(Actinidia chinensis Planch.) maturity using plastic house cultivation | |
| Erez | Container-grown trees: a new option for intensive culture of temperate zone fruit trees in the tropics | |
| Njovu | Effect of irrigation regimes on yield and quality of grapes (Vitis vinifera L. cv.‘Makutupora red’) in Dodoma, Tanzania |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191206 Termination date: 20200830 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |