CN107432879A - 侧柏叶生发活性成分的提取方法 - Google Patents
侧柏叶生发活性成分的提取方法 Download PDFInfo
- Publication number
- CN107432879A CN107432879A CN201610346738.2A CN201610346738A CN107432879A CN 107432879 A CN107432879 A CN 107432879A CN 201610346738 A CN201610346738 A CN 201610346738A CN 107432879 A CN107432879 A CN 107432879A
- Authority
- CN
- China
- Prior art keywords
- cacumen biotae
- lard
- extraction
- hair
- dose group
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 210000004209 hair Anatomy 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 16
- 230000001256 tonic effect Effects 0.000 title abstract description 12
- 238000000605 extraction Methods 0.000 claims abstract description 26
- 239000003814 drug Substances 0.000 claims abstract description 15
- 230000003656 anti-hair-loss Effects 0.000 claims abstract description 13
- 235000021388 linseed oil Nutrition 0.000 claims abstract description 13
- 239000000944 linseed oil Substances 0.000 claims abstract description 13
- 239000000284 extract Substances 0.000 claims abstract description 12
- 239000002904 solvent Substances 0.000 claims abstract description 12
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 244000134716 Hodgsonia macrocarpa Species 0.000 claims abstract description 5
- 235000001511 Hodgsonia macrocarpa Nutrition 0.000 claims abstract description 5
- 239000010699 lard oil Substances 0.000 claims abstract description 5
- 239000002537 cosmetic Substances 0.000 claims abstract description 4
- 235000013305 food Nutrition 0.000 claims abstract description 4
- 230000036541 health Effects 0.000 claims abstract description 4
- 238000004519 manufacturing process Methods 0.000 claims abstract description 3
- 239000000243 solution Substances 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 238000001816 cooling Methods 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000012085 test solution Substances 0.000 claims description 4
- 230000003402 anti-promotion Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000002844 melting Methods 0.000 claims description 2
- 230000008018 melting Effects 0.000 claims description 2
- 230000003779 hair growth Effects 0.000 abstract description 11
- 230000000694 effects Effects 0.000 abstract description 7
- 238000012545 processing Methods 0.000 abstract description 2
- 230000035617 depilation Effects 0.000 description 11
- 241000699666 Mus <mouse, genus> Species 0.000 description 8
- 210000003780 hair follicle Anatomy 0.000 description 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 241001465754 Metazoa Species 0.000 description 2
- 240000003243 Thuja occidentalis Species 0.000 description 2
- 235000008109 Thuja occidentalis Nutrition 0.000 description 2
- 239000002398 materia medica Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000010025 steaming Methods 0.000 description 2
- 206010011224 Cough Diseases 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 208000034507 Haematemesis Diseases 0.000 description 1
- 206010021703 Indifference Diseases 0.000 description 1
- 238000012449 Kunming mouse Methods 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- 206010053615 Thermal burn Diseases 0.000 description 1
- 206010046788 Uterine haemorrhage Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000011149 active material Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 208000001780 epistaxis Diseases 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000002070 germicidal effect Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 208000006750 hematuria Diseases 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 210000002429 large intestine Anatomy 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000013641 positive control Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 229910052979 sodium sulfide Inorganic materials 0.000 description 1
- GRVFOGOEDUUMBP-UHFFFAOYSA-N sodium sulfide (anhydrous) Chemical compound [Na+].[Na+].[S-2] GRVFOGOEDUUMBP-UHFFFAOYSA-N 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/13—Coniferophyta (gymnosperms)
- A61K36/14—Cupressaceae (Cypress family), e.g. juniper or cypress
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/35—Extraction with lipophilic solvents, e.g. Hexane or petrol ether
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- General Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Biotechnology (AREA)
- Public Health (AREA)
- Microbiology (AREA)
- Birds (AREA)
- Chemical & Material Sciences (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Cosmetics (AREA)
Abstract
本发明公开了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶剂和提取条件以及最新炮制加工工艺，提取的两种最佳溶剂分别是猪脂和亚麻籽油，提取最佳温度为110℃，提取液最佳浓度为0.25g/ml，提取最佳时间为30min；以及侧柏叶制成成品前进行蒸制处理的独创炮制工艺；此方法用于制备防脱发及生发药物、保健品、化妆品、食品等成本低廉，适用于工业化生产。
Description
技术领域
本发明公开了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶媒和提取条件以及最新炮制加工工艺，提取的两种最佳溶剂分别是猪脂和亚麻籽油，提取最佳温度为110℃，提取液最佳浓度为0.25g/ml，提取最佳时间为30min；以及侧柏叶制成成品前进行蒸制处理的独创炮制工艺。
背景技术
侧柏叶(Cacluneu Platycladi )又名丛柏叶，是侧柏的干燥嫩枝梢及叶；中医认为侧柏叶气清香，味苦涩，性微寒.无毒入心`,肝、大肠经；具有凉血、止血、除内湿、散肿毒之功；可治吐血、鼻出血、尿血、血痢、崩漏、风湿痛咳嗽、烫伤等症；明代李时珍《本草纲目》中记载侧柏叶“乃多寿之木，所以可以入服食；道家以之点汤常饮；”侧柏叶作为传统的中药，其预防脱发和生发的功能一直被人忽略；而在诸多古代文献中记载其主要功能为乌发和生发，在《本草纲目》中谓其：浸油，生发，烧汁，黑发；和猪脂，沐发长黑；然而侧柏叶的生发物质基础却尚不明确，经检索关于侧柏叶中的生发活性物质未见报道。
发明内容
本发明公开了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶媒和提取条件并制定了质量标准；公开了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶媒和提取条件以及最新炮制加工工艺，此方法用于制备防脱发及生发药物、保健品、化妆品、食品等成本低廉，适用于工业化生产;
本发明所述的最佳提取溶剂和提取条件，其特征在于：
是以猪脂和亚麻籽油作为溶媒，提取最佳温度为110℃，提取液最佳浓度为0.25g/ml，提取最佳时间为30min的提取工艺；
本发明所述的所述从胶陀螺中提取的光敏活性物质的提取方法，包括以下步骤：
1)制备猪脂提取液
首先把猪脂放于锅内加热融化成液态，分别称取75ml、85ml、95 ml放于烧杯杯内；取蒸制后的侧柏叶粉碎过80目筛备用；分别制成猪脂混合物高剂量组（0.25g/ml），中剂量组（0.15g/ml），低剂量组（0.05g/ml）的受试液100ml；然后放于电磁炉上加热，温度始终控制在110℃，持续加热30min后，取出冷却；
2)制备亚麻籽油提取液
首先把亚麻籽油放于锅内加热后，分别称取75ml、85ml、95 ml放于烧杯杯内；取蒸制后的侧柏叶粉碎过80目筛备用；分别制成猪脂混合物高剂量组（0.25g/ml），中剂量组（0.15g/ml），低剂量组（0.05g/ml）的受试液100ml；然后放于电磁炉上加热，温度始终控制在110℃，持续加热30min后，取出冷却；
本发明的积极效果在于：本发明公开了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶剂和提取条件以及最新炮制加工工艺，此方法用于制备防脱发及生发药物、保健品、化妆品、食品等成本低廉，具有明显的临床效果；
试验例：
通过以下试验证明本发明提取的物质是生发活性成分：
1.材料
1.1本发明提取的生发活性成分；
1.2受试动物：昆明种小鼠,体重 20-22g, 购自吉林大学实验动物中心；
2.试验方法：
2.1硫化钠脱毛模型的建立
健康成年昆明雄性SD小鼠80只，用基础饲料喂养3-5d，用剪刀剪去其背部的毛发，面积约2X3cm，然后涂上浓度为8%的硫化钠溶液，过5分钟后，用温的蒸馏水洗净，防止烫伤皮肤；此方法诱导可以高度同步的生长期毛囊的发育,该毛囊与自发形成的生长期毛囊在形态上无差别；
2.2实验动物分组及给药
2.2.1硫化钠脱毛模型动物分组及给药
对小鼠采用硫化钠脱毛模型,从而研究侧柏叶对促进正常毛发生长的作用;至于侧柏叶能否减弱因环磷酰胺引起的毛囊损伤导致的脱毛,从而起到预防脱毛的的作用,将由同时进行的预防脱毛作用试验进行研究；
选取脱毛区光滑且无皮肤破损的小鼠,随机分为8组，分别为：阴性对照组、猪脂高剂量组（Lard-G）、猪脂中剂量组（Lard-G）、猪脂低剂量组（Lard-G）、亚麻籽油高剂量组(Linseed-G) 、亚麻籽油中剂量组(Linseed-Z)、亚麻籽油低剂量组(Linseed-D)、阳性对照组；
实验小鼠正常饲养,从脱毛后第2天背部涂抹受试液及阳性药物0.3mL，用于促进毛发生长作用实验的小鼠背部连续涂抹给药12天；
2.2.2实验观察及评价方法
对于化疗模型组的小鼠逐日观察脱毛情况,并按照未脱毛记0分，头颈及背部稀疏脱毛记1分，头颈及背部中度脱毛记2分，头颈及背部明显脱毛记3分的标准对小鼠脱毛情况进行打分,结果进行秩和检验(脱毛积分)分析；对于促进毛发生长的小鼠无毛发生长记0分，有少量细小绒毛出现记1分，有较明显毛发出现记2分，毛发生长良好的记3分，毛发生长同正常小鼠无异记4分，所述状况显著记“*”;
结论
通过实验研究发现了中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶媒和提取条件，提取的两种最佳溶媒分别是猪脂和亚麻籽油，提取最佳温度为110℃，提取液最佳浓度为0.25g/ml，提取最佳时间为30min；侧柏叶作为药食两用的中药，具有非常明显的促进毛发生长、预防脱发、生发、乌发等作用；侧柏叶可以被开发为保健食品、药品、洗护用品等，使侧柏叶广泛受益于人类。
Claims (2)
1.中药侧柏叶以猪脂和亚麻籽油，提取最佳温度为110℃，提取液最佳浓度为0.25g/ml，提取最佳时间为30min的提取工艺方法。
2.根据权利要求1所述的应用，侧柏叶的猪脂和亚麻籽油提取物的制备过程如下：
猪脂组：首先把猪脂放于锅内加热融化成液态，分别称取75ml、85ml、95 ml放于烧杯杯内；取侧柏叶粉碎过80目筛备用；分别制成猪脂混合物高剂量组（0.25g/ml），中剂量组（0.15g/ml），低剂量组（0.05g/ml）的受试液100ml；然后放于电磁炉上加热，温度始终控制在110℃，持续加热30min后，取出冷却；
亚麻籽油组：首先把亚麻籽油放于锅内加热后，分别称取75ml、85ml、95 ml放于烧杯杯内；取侧柏叶粉碎过80目筛备用；分别制成猪脂混合物高剂量组（0.25g/ml），中剂量组（0.15g/ml），低剂量组（0.05g/ml）的受试液100ml；然后放于电磁炉上加热，温度始终控制在110℃，持续加热30min后，取出冷却;权利要求1所述的中药侧柏叶预防脱发和促进毛发生长作用的最佳提取溶剂和提取条件以及最新炮制加工工艺，此方法用于制备防脱发及生发药物、保健品、化妆品、食品等成本低廉，适用于工业化生产。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610346738.2A CN107432879A (zh) | 2016-05-24 | 2016-05-24 | 侧柏叶生发活性成分的提取方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610346738.2A CN107432879A (zh) | 2016-05-24 | 2016-05-24 | 侧柏叶生发活性成分的提取方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107432879A true CN107432879A (zh) | 2017-12-05 |
Family
ID=60452863
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610346738.2A Pending CN107432879A (zh) | 2016-05-24 | 2016-05-24 | 侧柏叶生发活性成分的提取方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107432879A (zh) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464317A (zh) * | 2018-12-14 | 2019-03-15 | 上海辉文生物技术股份有限公司 | 一种含侧柏提取物的组合物及其制备方法和应用 |
| CN112057371A (zh) * | 2020-09-27 | 2020-12-11 | 新疆大德恒生物股份有限公司 | 一种杏仁育发乳、其组合物及其制备方法 |
| CN115245526A (zh) * | 2022-08-05 | 2022-10-28 | 劲牌持正堂药业有限公司 | 一种侧柏叶提取物的脱苦方法及其在保健酒中的应用 |
-
2016
- 2016-05-24 CN CN201610346738.2A patent/CN107432879A/zh active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109464317A (zh) * | 2018-12-14 | 2019-03-15 | 上海辉文生物技术股份有限公司 | 一种含侧柏提取物的组合物及其制备方法和应用 |
| CN112057371A (zh) * | 2020-09-27 | 2020-12-11 | 新疆大德恒生物股份有限公司 | 一种杏仁育发乳、其组合物及其制备方法 |
| CN115245526A (zh) * | 2022-08-05 | 2022-10-28 | 劲牌持正堂药业有限公司 | 一种侧柏叶提取物的脱苦方法及其在保健酒中的应用 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105943466B (zh) | 一种婴童洗发沐浴露 | |
| CN107432879A (zh) | 侧柏叶生发活性成分的提取方法 | |
| Ryman | The aromatherapy handbook: the secret healing power of essential oils | |
| CN102277228A (zh) | 一种精制鸵鸟油的制备方法及用途 | |
| CN103305330A (zh) | 芍药属芍药组植物种子油及其制备方法和用途 | |
| CN102335103A (zh) | 具有驱蚊作用的芳香精油 | |
| KR102275549B1 (ko) | 산수국 추출물을 포함하는 스트레스 완화 및 심리적 안정 증진용 향료 조성물 | |
| KR101731079B1 (ko) | 한방오리백숙 제조방법 및 한방오리백숙 | |
| CN104323948A (zh) | 一种祛斑复方精油 | |
| CN110477397B (zh) | 一种制备石斛花提取液的方法及其应用 | |
| CN105533028A (zh) | 一种具有养颜养血功效的当归玫瑰速溶茶的制备方法 | |
| CN105432895A (zh) | 一种具有滋肾养颜功效的玫瑰枸杞速溶茶的制备方法 | |
| KR101758904B1 (ko) | 강황, 하고초, 참마 및 뽕나무 복합추출물을 함유하는 슬리밍용 화장료 조성물 | |
| CN108578286A (zh) | 一种臀部塑形精油及其制作方法 | |
| CN107484777A (zh) | 一种艾叶驱蚊虫汁 | |
| CN111067839A (zh) | 一种调理修护剂及其制备方法 | |
| CN103006515B (zh) | 一种天然植物护发喷剂及制备方法 | |
| KR20170043071A (ko) | 부들(Typha orientalis)추출물을 포함하는 화장수 제조 방법 | |
| CN104352572B (zh) | 一种女贞子的炮制方法 | |
| CN104225081A (zh) | 一种用野山茶油防治心血管疾病的药物及其制备方法 | |
| KR20160123538A (ko) | 유황동충하초황계탕, 유황땅콩새싹황계탕, 유황삼황계탕 제조방법 | |
| CN105326755B (zh) | 无添加剂绿豆祛痘养护面膜纸及其绿色生产方法 | |
| CN104490642B (zh) | 天然虫白蜡护发生发剂及其制备方法 | |
| CN103719502B (zh) | 一种梁王茶制备茶叶的方法 | |
| CN105558190A (zh) | 一种具有补血养颜功效的玫瑰阿胶速溶茶的制备方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171205 |
|
| RJ01 | Rejection of invention patent application after publication |