CN107430139A - Automatic analysing apparatus and automatic analysis method - Google Patents

Automatic analysing apparatus and automatic analysis method Download PDF

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Publication number
CN107430139A
CN107430139A CN201680016754.3A CN201680016754A CN107430139A CN 107430139 A CN107430139 A CN 107430139A CN 201680016754 A CN201680016754 A CN 201680016754A CN 107430139 A CN107430139 A CN 107430139A
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China
Prior art keywords
plasma
sample
normal
blood plasma
described
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Application number
CN201680016754.3A
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Chinese (zh)
Inventor
薮谷千枝
铃木直人
牧野彰久
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株式会社日立高新技术
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Priority to JP2015057503 priority Critical
Priority to JP2015-057503 priority
Application filed by 株式会社日立高新技术 filed Critical 株式会社日立高新技术
Priority to PCT/JP2016/054058 priority patent/WO2016152305A1/en
Publication of CN107430139A publication Critical patent/CN107430139A/en

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    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/86Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood coagulating time or factors, or their receptors
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/17Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/25Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
    • G01N21/27Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration
    • G01N21/272Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands using photo-electric detection ; circuits for computing concentration for following a reaction, e.g. for determining photometrically a reaction rate (photometric cinetic analysis)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using infra-red, visible or ultra-violet light
    • G01N21/75Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/77Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator
    • G01N21/82Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated by observing the effect on a chemical indicator producing a precipitate or turbidity
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • G01N33/4905Determining clotting time of blood
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/96Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood or serum control standard
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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    • G01N35/0095Scheduling introducing urgent samples with priority, e.g. Short Turn Around Time Samples [STATS]
    • GPHYSICS
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    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/025Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations having a carousel or turntable for reaction cells or cuvettes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1002Reagent dispensers
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38Diluting, dispersing or mixing samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00346Heating or cooling arrangements
    • G01N2035/00356Holding samples at elevated temperature (incubation)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465Separating and mixing arrangements
    • G01N2035/00534Mixing by a special element, e.g. stirrer
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465Separating and mixing arrangements
    • G01N2035/00534Mixing by a special element, e.g. stirrer
    • G01N2035/00544Mixing by a special element, e.g. stirrer using fluid flow
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N2035/00465Separating and mixing arrangements
    • G01N2035/00534Mixing by a special element, e.g. stirrer
    • G01N2035/00554Mixing by a special element, e.g. stirrer using ultrasound
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers
    • G01N35/00722Communications; Identification
    • G01N35/00732Identification of carriers, materials or components in automatic analysers
    • G01N2035/00821Identification of carriers, materials or components in automatic analysers nature of coded information
    • G01N2035/00831Identification of carriers, materials or components in automatic analysers nature of coded information identification of the sample, e.g. patient identity, place of sampling
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/00584Control arrangements for automatic analysers
    • G01N35/00722Communications; Identification
    • G01N2035/00891Displaying information to the operator
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
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    • G01N2035/0094Scheduling optimisation; experiment design
    • GPHYSICS
    • G01MEASURING; TESTING
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    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0439Rotary sample carriers, i.e. carousels
    • G01N2035/0441Rotary sample carriers, i.e. carousels for samples
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • G01N35/04Details of the conveyor system
    • G01N2035/0439Rotary sample carriers, i.e. carousels
    • G01N2035/0444Rotary sample carriers, i.e. carousels for cuvettes or reaction vessels

Abstract

Present invention offer is a kind of can to make that the analytical equipment prepared automatically and method will be realized for examination blood plasma and the pooled plasma that normal plasma mix with defined mixing ratio.Automatic analysing apparatus (100) possesses:Sample dispensing mechanism (101), it dispenses the normal plasma added for examination blood plasma and/or to correct the setting time for trying blood plasma to multiple sample containers (103);Reaction vessel (104), it is used for housing for examination blood plasma and/or normal plasma;Reagent dispensing mechanism (106), it dispenses reagent to reaction vessel (104);And detection unit (113), it for examination blood plasma and/or normal plasma after reagent is with the addition of in reaction vessel (104) to irradiating the light from light source (115), and the scattering light based on acquisition and/or transmission light measurement setting time.Sample dispensing mechanism (101) will be configured to pooled plasma only for trying blood plasma, only normal plasma and being dispensed so that at least one kind of mixing ratio is mixed for examination blood plasma and normal plasma into multiple sample containers (103).

Description

Automatic analysing apparatus and automatic analysis method

Technical field

The present invention relates to it is a kind of to the biological samples such as blood, urine carry out qualitative and quantitative analysis automatic analysing apparatus, especially It is to be related to the automatic analysing apparatus and automatic analysis method that a kind of solidification suitable for blood and hemostasis check.

Background technology

The purpose of blood clotting inspection is the morbid state of assurance blood clotting fibrinolytic system, (dispersivity is intravascular by diagnosis DIC Blood coagulation), confirm thrombus treatment effect and diagnosis hemophilia.Especially, blood coagulation time measure is to mix sample and reagent Close, determine the time (hereinafter referred to as blood coagulation time) untill fibrin clot is formed, appearance is congenital, posteriority is different Chang Shi, blood coagulation time can extend.

But only determine blood coagulation time and can not differentiate that its reason is due to a lack of living caused by blooc coagulation factor Property reduce (deficient) or because antibody for form blood clotting system composition or blood coagulation time measure reagent in Activity reduces caused by the blood coagulation reaction of composition etc. suppresses (suppression formulation).

On the other hand, in the treatment, it is deficient according to the extension reason of blood coagulation time or suppresses formulation, treats policy Can be different, it is therefore necessary to its clear and definite reason.

The method of the reason for as differentiating the extension of blood coagulation time, have by adding normal plasma to carry out Cross-mixing experiment (Cross mixing test) (also referred to as blood clotting correction test or cross-mixing experiment).Intersect mixed Close in experiment, normal plasma can be added in for examination blood plasma, and the degree of correction of the blood coagulation time is depicted as chart and entered Row judges.The judgement for the extension factor that the most representational use example of cross-mixing experiment is APTT, but sometimes also can be at it He is PT (prothrombin time), dPT (dilution PT), dAPTT (dilution APTT), KCT (kaolin clotting time) and dRVVT Implement in projects such as (dilution russell's viper venom times).

But even if APTT is the main project that can implement in the most facilities for implementing blood clotting inspection, but it is existing It is being difficult frequent implementation cross-mixing experiment.When can not implement in facility and need outside ministries and commissions' support object delegate inspection, need Spend more time to obtain result, therefore for serious diseases such as hemophilia, the state of an illness can be resulted in a finding that and start to treat Delay.The reason for producing such a situation is that the preparation of sample and the explanation of culture operation complexity and its result are also and unclear Chu, it is therefore desirable to skilled examiner.

In order to solve the above problems, it is proposed that patent document 1.In patent document 1, for only for examination blood plasma, only normal blood Slurry and the sample (pooled plasma) that will be mixed with least one kind of mixing ratio for examination blood plasma and normal plasma determine blood respectively Liquid setting time, calculate by the measured value obtained draw below the line chart that forms product (A) with will only for examination blood plasma with only The difference of product (B) below the straight line that the measured value of normal plasma is formed by connecting, and to area ratio (A-B)/(B) of the difference with Defined reference area is compared than Y, based on comparative result, is determined that it is and is suppressed formulation or shortage type.

Prior art literature

Patent document

【Patent document 1】WO2009/153964 publications

The content of the invention

The technical problems to be solved by the invention

But the method for making the mixing for examination blood plasma and normal plasma realize automation is not disclosed in patent document 1, When being prepared using manual methods, the mixed of acquisition may be made due to the complication of operation or the proficiency of operating personnel Deviation be present in the precision for closing the mixing ratio of blood plasma.

Therefore, present invention offer is a kind of can make what will be mixed with defined mixing ratio for examination blood plasma with normal plasma Pooled plasma realizes the automatic analysing apparatus and automatic analysis method prepared automatically.

Solves the technical scheme of technical problem

In order to solve above-mentioned problem, automatic analysis apparatus of the present invention is characterised by possessing:Sample dispenses mechanism, its The normal plasma added for examination blood plasma and/or to correct the setting time for examination blood plasma is dispensed to multiple samples and held Device;Reaction vessel, it prepares only described for examination blood plasma, only described normal plasma and with least one kind of in the sample container The pooled plasma mixed for trying blood plasma and normal plasma is utilized dispensing institute of sample dispensing mechanism by mixing ratio State confession examination blood plasma, the normal plasma and the pooled plasma being formulated;Reagent dispenses mechanism, and it divides reagent Note to the reaction vessel;And determination part, it is to the addition of the described for examination blood plasma, normal blood of reagent in the reaction vessel The light of slurry and/or pooled plasma irradiation from light source, and the scattering light based on acquisition and/or transmission light measurement setting time.

In addition, the automatic analysis method of the present invention is characterised by, dispensing mechanism using sample will be for trying blood plasma and/or being The normal plasma for correcting the setting time for examination blood plasma and adding is dispensed to multiple sample containers, and in the sample container Interior preparation is only described to supply to try blood plasma and normal blood for examination blood plasma, only described normal plasma and with least one kind of mixing ratio by described The pooled plasma mixed is starched, by confession examination blood plasma, the normal plasma and the mixing blood being formulated Slurry is housed into reaction vessel, while is dispensed reagent into the reaction vessel using reagent dispensing mechanism, to described The light from light source for examination blood plasma and/or normal plasma irradiation of reagent is with the addition of in reaction vessel, and based on acquisition Scatter light and/or transmission light measurement setting time.

Invention effect

In accordance with the invention it is possible to provide one kind can make with defined mixing ratio will for examination blood plasma be mixed with normal plasma and Into pooled plasma realize the automatic analysing apparatus and automatic analysis method prepared automatically.

Problem, structure and effect other than the above can be according to the explanations of implementation below come clearly.

Brief description of the drawings

Fig. 1 is the overall schematic configuration diagram of the automatic analysing apparatus of the embodiment 1 involved by one embodiment of the present invention.

Fig. 2 is the skeleton diagram of cross-mixing experiment.

Fig. 3 is the flow chart for the handling process for showing the automatic analysing apparatus shown in Fig. 1.

Fig. 4 is the display example of operation screen when entrusting cross-mixing experiment measure.

Fig. 5 is the display example of operation screen when entrusting cross-mixing experiment measure.

Fig. 6 is to show the figure using state during sample dispensing mechanism suction normal plasma.

Fig. 7 is the figure for showing using the mechanism suction of sample dispensing state during for examination blood plasma.

Fig. 8 is the figure for showing state during using sample dispensing mechanism discharge normal plasma or for examination blood plasma.

Fig. 9 is the figure for showing the state that blood plasma is stirred using rabbling mechanism.

Figure 10 is the figure for the cross-mixing result of the test for showing the automatic analysing apparatus progress using embodiment 1.

Figure 11 is the overall schematic configuration diagram of the automatic analysing apparatus of the embodiment 2 involved by the other embodiment of the present invention.

Figure 12 is the explanation figure of the conveyance order of sample rack in automatic analysing apparatus shown in Figure 11.

Figure 13 is the explanation figure of the conveyance order of sample rack when cross-mixing is tested in automatic analysing apparatus shown in Figure 11.

Figure 14 is the explanation figure of sample dispensing position when cross-mixing is tested in automatic analysing apparatus shown in Figure 11.

Figure 15 is the stream of the handling process for the automatic analysing apparatus for showing the embodiment 3 involved by the other embodiment of the present invention Cheng Tu.

The display example of operation screen when Figure 16 is the commission cross-mixing experiment of embodiment 3.

Figure 17 is the stream of the handling process for the automatic analysing apparatus for showing the embodiment 4 involved by the other embodiment of the present invention Cheng Tu.

Figure 18 is the timing diagram of the action for the automatic analysing apparatus for showing embodiment 4.

Figure 19 is the stream of the handling process for the automatic analysing apparatus for showing the embodiment 5 involved by the other embodiment of the present invention Cheng Tu.

Figure 20 is the stream of the handling process for the automatic analysing apparatus for showing the embodiment 6 involved by the other embodiment of the present invention Cheng Tu.

The display example of operation screen when Figure 21 is the commission cross-mixing experiment of embodiment 6.

Figure 22 is to show only normal plasma, the figure only for trying blood plasma and the pooled plasma mixed according to 5 kinds of mixing ratios.

Figure 23 is to show only normal plasma, the figure only for trying blood plasma and the pooled plasma mixed according to 3 kinds of mixing ratios.

Figure 24 is to show only normal plasma, the figure only for trying blood plasma and the pooled plasma mixed according to a kind of mixing ratio.

Embodiment

In this specification, include being in hospital " for examination blood plasma " or the blood of the subject such as the blood plasma of out-patient and Gernral Check-up Slurry.In addition, in this specification, as " normal plasma ", " for examination blood plasma " and " the mixing blood mixed with various mixing ratios The general designation of slurry ", it is referred to as being used for the sample for determining blood coagulation time sometimes.In addition, in this specification, " general sample " refers to The sample of subject.

Fig. 2 is the skeleton diagram of cross-mixing experiment.Normal plasma is added in for examination blood plasma, and carries out mixed preparing, is made The ratio of normal plasma is 0,10,20,50,80,90,100%, prepares so-formed sample, implements APTT measure.Draw Measurement result (blood coagulation time) and the proportionate relationship of normal plasma, are made chart.As shown in Fig. 2 transverse axis is normal plasma Ratio (%), the longitudinal axis are APTT (blood coagulation time).For example, during deficient shown in Fig. 2 solid line (a), by adding just Normal blood plasma extends to correct APTT, is shown as downwardly convex pattern.On the other hand, when suppressing formulation, such as Fig. 2 solid line (b) It is shown, also it is difficult to correct APTT extensions even if addition normal plasma, is shown as the pattern to raise up.But inhibitor for The reaction of the VIII factor has time and temperature dependency, therefore in firm mixed reaction (hereinafter referred to as immediate reaction) not The shape to raise up can be explicitly shown, cultivates in the reaction (hereinafter referred to as deferred reaction) after certain time, has at 37 DEG C When can be shown as the shape that raises up.

Therefore, in cross-mixing experiment, it is proposed that while determine immediate reaction and deferred reaction.

Hereinafter, using brief description of the drawings embodiments of the present invention.

【Embodiment 1】

Fig. 1 is the overall schematic configuration diagram of the automatic analysing apparatus of the embodiment 1 involved by one embodiment of the invention.This Place, the flow of basic blood clotting inspection is illustrated using Fig. 1, but be not limited to the example below.

Automatic analysing apparatus 100 substantially by sample dispense mechanism 101, sample disk 102, reagent dispense mechanism 106, reagent disc 107, Reaction vessel storage unit 111, reaction vessel transport mechanism 112, detection unit 113, reaction vessel waste part 117, operating portion 118th, storage part 119 and control unit 120 are formed.

Sample dispensing mechanism 101 can aspirate the sample being housed in sample container 103, and be expelled to and be housed in reaction vessel In the reaction vessel 104 of storage unit 111, the sample container 103 is configured in clockwise and rotate counterclockwise sample disk 102. The leading section of sample dispensing mechanism 101 possesses sample dispensing probe 101a, passes through the sample syringe pump controlled by control unit 120 105 action, perform the suction action and discharging operation of sample.

The reagent being housed in reagent container 108 aspirates in reagent dispensing mechanism 106, and is expelled to and is housed in reaction vessel storage In the reaction vessel 104 for depositing portion 111, the reagent container 108 is configured in reagent disc 107.Reagent dispenses the leading section of mechanism 106 Possess reagent dispensing probe 106a, by the action by the reagent that control unit 120 controls with syringe pump 110, perform taking out for reagent Suction action and discharging operation.

In addition, reagent dispensing mechanism 106 it is built-in reagent heating-up mechanism 109.The temperature of the reagent aspirated by reagent dispensing mechanism 106 Degree is warming up to proper temperature (defined temperature) by the reagent heating-up mechanism 109 controlled by control unit 120.

Reaction vessel transport mechanism 112 is implemented conveyance to the reaction vessel 104 for being housed in reaction vessel storage unit 111 and set Put.Reaction vessel transport mechanism 112 is by gripping reaction vessel 104 and being rotated in the horizontal plane in arc-shaped, by reaction vessel 104 transport and are arranged at the reaction vessel setting unit 114 of detection unit 113 from reaction vessel storage unit 111.

Detection unit 113 has reaction vessel setting unit 114 more than at least one, and it is used for loading reaction vessel 104. Detection unit 113 is to the sample measures luminous intensity in the reaction vessel 104 of intercalation reaction container setting unit 114.In addition, this implementation In example, it is shown that the situation of 1 detection unit 113 of configuration, but can also be configured to that there are multiple detection units 113.Detection is single The example of Cleaning Principle in member 113 is as described below.The light irradiated from light source 115 can be molten because of the reaction in reaction vessel 104 Liquid and scatter.Test section (light accepting part) 116 is made up of photodiode etc..Test section 116 is by receiving because of reaction vessel Reaction solution (sample) in 104 and the scattering light that scatters simultaneously implement optical electrical conversion, will represent received scattering light The light signal of intensity is exported to A/D converter 121.Implement the measure of the scattering light after A/D conversions by A/D converter 121 Signal can be inputted by interface 122 to control unit 120.The action of detection unit 113 is controlled by control unit 120.Herein, control Portion 120 processed is made up of analysis operation control part 120a and operational part 120b.Analyze operation control part 120a and operational part 120b examples Such as realized by CPU processor, by reading the various programs being stored in ROM or storage part 119 (not shown) and transporting The read program of row, implements control and computing.

That is, analysis operation control part 120a control sample dispensing mechanisms 101 and sample disk 102, implement sample dispensing.This Outside, analysis operation control part 120a also controls reagent dispensing mechanism 106 and reagent disc 107, and reagent is expelled into reaction vessel 104 Interior sample.Also, analysis operation control part 120a can also control the movement of reaction vessel 104, reaction vessel 104 it is discarded etc. The action of automatic analysing apparatus.

Operational part 120b is based on basis and the degree of sample and the hybrid reaction of the reagent light intensity that accordingly time of origin changes The comparative result of the signal value that the measured value of degree obtains and pre-determined decision threshold, implement measure processing, determine sample Reaction time.The setting time calculated can be stored to storage part 119 while output is to display part 118c.In addition, also may be used So that by interface 122, the setting time as result of calculation is printed out to printer 123.

The composition of test section 116 is not limited only to receive dissipate caused because of the reaction solution (sample) in reaction vessel 104 Penetrate light.For example, it is also possible to test section 116 is configured to transmission of the detection through the reaction solution (sample) in reaction vessel 104 The intensity of light.In addition it is also possible to using simultaneously using the test section of above-mentioned scattering light detection mode and transmitted light detection mode 116.Also, it in addition to the foregoing, can also use the detector 116 using viscosity.

Reaction vessel transport mechanism 112 grips the reaction vessel 104 after measure terminates, and is discarded to reaction vessel waste part 117。

In addition, in order to improve disposal ability, also it may be configured as, sample can be heated before addition determines beginning reagent and not have by possessing There is the insulating box 124 of detector.

The analysis project for the sample analyzed by the use of automatic analysing apparatus 100 can be by being used as the keyboard 118b of input unit and showing Show that the operation screen on display part 118c is inputted to control unit 120 from operating portion 118.Alternatively, it is also possible to being to use GUI The composition of (Graphical User Interface, graphic user interface), the GUI can utilize the behaviour such as pointer by mouse 118a Make the analysis project shown in display part 118c, input analysis project.

In addition, in Fig. 1, for the ease of showing all inscapes, labeled as reaction vessel storage unit 111, sample disk 102 And the separate configuration of reagent disc 107, but actually sample disk 102 is configured in composition sample dispensing with reaction vessel storage unit 111 In the range of the arc-shaped motion tracks of the sample dispensing probe 101a of mechanism 101 in the horizontal plane.In addition, reagent disc 107 with Reaction vessel storage unit 111, which configures, is forming the circular arcs of the reagent dispensing probe 106a of reagent dispensing mechanism 106 in the horizontal plane In the range of shape motion track.Therefore, during vertical view, these sample disks 102, reaction vessel storage unit 111 and reagent disc 107 are in Roughly triangular shape configures.

Then, the commission of the cross-mixing experiment of the automatic analysing apparatus 100 of the present embodiment described further below and sample Compound method.Fig. 3 is the flow chart for the handling process for showing the automatic analysing apparatus shown in Fig. 1, especially shows that commission intersects The flow of sample compound method during bulk testing.

First, automatic analysing apparatus 100 accepts the commission (step S101) of cross-mixing experiment.The acceptance method of commission is divided into logical Cross the mode received using the network system of master computer and entrust measure to accept from operating portion 118 by operator The mode of the cross-mixing experiment measure commission inputted.One is used as below, illustrates to input measure commission by operation screen Situation.

Fig. 4 and Fig. 5 represents to be shown in the display part 108c for forming operating portion 118 during commission cross-mixing experiment measure The display example of operation screen.As shown in figure 4, cross-mixing experiment measure commission picture (operation screen) has the kind of display sample Class is its region for which of general sample, urgent sample and management product.It is shown in Fig. 4 for general sample Cross-mixing measure commission.In addition, cross-mixing measure commission picture (operation screen) have inspection project select/specify area Domain 127.Operator can specify from inspection project selection/designated area 127 implements cross-mixing examination by the operation screen The project tested.In example shown in Fig. 4, it is shown that selection specifies project APTT state.In addition, operation screen have it is optional Select the region of specified normal plasma ratio.In example shown in Fig. 4, it is shown that the ratio of normal plasma is set as 0,10,20, 50th, the state of 80,90,100% this all 7 kinds of condition.In addition, the normal plasma ratio set herein is not limited only to shown in Fig. 4 7 kinds of conditions, as long as such as be set as more than 3 kinds of conditions containing 0%, 100%, i.e. only for examination blood plasma, only normal plasma with And the pooled plasma mixed with least one kind of mixing ratio, other mixing ratios can arbitrarily be set.

As shown in figure 4, after setting measure project and normal plasma ratio, analysis operation control part 120a can calculate measure institute Need normal plasma amount and for examination plasma volume, according to each condition determine respectively different normal plasma amounts and for examination plasma volume, and Control the action of sample dispensing mechanism 101.Now, as shown in figure 5, by showing required normal plasma amount in display part 118c With for trying plasma volume, operator is signaled to.When operator can hold required plasma volume, it can not only mitigate operator's calculating The desired amount of burden, while the effect for preventing occurring plasma volume deficiency in the midway of preparation in advance can also be obtained.Herein, Fig. 5 Middle operator specifies normal plasma, for examination blood plasma, the set location of empty sample container, but is also controllable to device and specifies setting Position.In addition, in Fig. 5, it is shown that the set location of normal plasma is set as into " 100 ", will be set for the set location for trying blood plasma It is set to " 101 " and the starting position of empty sample container is set as to the state of " 102 ".Herein, each position represents sample disk The position of sample container 103 in 102, not it is only capable of being specified with numeral, such as can be also referred to by letter with number combinatorics on words Determine the position of sample container 103.

In addition, from when accepting commission using the network system of master computer, can need not setting measure project and condition determination Analyzed.

Under the state shown in fig. 5, after pressing START button (step S102), normal plasma amount is confirmed, for trying blood plasma Measure and whether there is sky sample container (step S103).Herein, empty sample container refers to have the disposable of individual identification media can The container clogged.Individual identification media refer to the media for identifying sample, such as bar code or RFID etc. can be used.Subject In the individual identification media of sample, in addition to the sample ID for identifying sample, also containing measure entrusted information etc..Paste In individual identification media in empty sample container, be assigned with arbitrary numbers, for manage by normal plasma, for examination blood plasma mix and Into pooled plasma.Confirm normal plasma amount, for examination the presence or absence of plasma volume and empty sample container when, sample dispensing machine can be utilized The liquid level detection function of structure 101, dispense probe 101a contacts using the sample of the front end because being arranged on sample dispensing mechanism 101 Or the change close to liquid level and the electrical characteristic such as static capacity or resistance value for changing, detect liquid level.In addition, also it may make up To be shot using the camera function (sensor such as CCD, CMOS, PMT) of miniature camera etc., being calculated according to liquid level Liquid measure.By the use of the liquid level detection function of sample dispensing mechanism 101 and bar code is used to know as individual as an explanation below The situation of other media.

With the rotation of clockwise and rotate counterclockwise sample disk 102, the subject for the position specified in Fig. 5 is arranged on When sample and empty sample container are by before reading part 125, the individual identification media i.e. bar code of subject's sample can be read.It is right Checked in the corresponding authorized item of subject's sample with being specified according to the bar code read, and will be set The normal plasma ratio and original subject's sample information and the ID of each pooled plasma made in empty sample container is checked. Then, as shown in fig. 6, the sample container 103a that normal plasma is filled with the sample container 103 in sample disk 102 is moved to Sample dispenses the dispensing position of mechanism 101, and confirms normal plasma using sample dispensing probe 101a liquid level detection function Amount.Similarly, the sample container 103b being filled with for examination blood plasma can be moved to dispensing position, and utilize sample dispensing probe 101a liquid level detection function, confirm the amount (Fig. 7) for examination blood plasma.And for empty sample container, probe is dispensed by sample 101a liquid level detection function, when not contacting and being contacted to the bottom of the sample container (abnormal to decline detection) with liquid level, know Not Wei set sample container be empty.

In return Fig. 3, step S104, container sets the result checked for normal plasma amount, is not up to institute for examination plasma volume Requirement or not assigned position set needed for number empty sample container when, stop the preparation of pooled plasma, and display system Alarm (step S105).Therefore, it is possible to avoid measure midway from blood plasma deficiency occur or due to being not provided with sky sample container The sample container that position has dispensed sample (normal plasma, for examination blood plasma or pooled plasma) dispenses and makes sample again The risk that disk 102 pollutes.

In step S104, prepare more than aequum normal plasma amount, for examination plasma volume and confirmed empty sample container There is provided during required number, start to the empty sample container dispensing normal plasma (step S106) of beginning.

Herein, the dispensing action of normal plasma is illustrated.With turning clockwise and the rotation of the sample disk of rotate counterclockwise 102, The sample container 103a for being filled with normal plasma is moved to dispensing position, and sample dispensing mechanism 101 can aspirate normal blood Starch (Fig. 6).In example shown in Fig. 6, it is shown that the situation of the stepped rotation counterclockwise of sample disk 102, the movement of each ladder Spacing of the distance equivalent to 2 sample containers 103 being adjacent to.Therefore, read and pasted as described above using reading part 125 Bar code in sample container 103, after sample ID is identified, along the direction of rotation of sample disk 102, positioning is extremely located at reading part The underface (sample dispensing position) of the sample dispensing mechanism 101 in 125 front.That is, each sample container 103 all the time can After sample ID is identified by reading part 125, positioning to dispensing position.

Then, with the stepped rotation of sample disk 102, positioning to the sample container 103 of dispensing position is to be filled with confession The sample container 103b (Fig. 7) of blood plasma is tried, therefore the sample dispensing probe 101a by forming sample dispensing mechanism 101 will not be discharged The normal plasma pumped out.Then, sample container of the positioning to dispensing position is empty sample container 103c, can discharge and be pumped to sample Normal plasma (Fig. 8) in this dispensing probe 101a.Repeat this action, by normal plasma dispense to empty sample container 103d~ 103i.After the dispensing of normal plasma completes (step S107), the dispensing (step S108) for examination blood plasma is then carried out.

Dispensing action in step S108 for examination blood plasma is that, with the rotation of sample disk 102, will be filled with first for examination blood The sample container 103b of slurry is positioned to dispensing position, and is supplied using the sample dispensing probe 101a suctions of sample dispensing mechanism 101 Try blood plasma (Fig. 7).Then, with the rotation of sample disk 102, positioning to the sample container of dispensing position is to arrange in step s 106 The sample container 103c of normal plasma is gone out.The blood plasma that supplies to try that sample dispenses probe 101a and will aspirated is expelled to sample container 103c (Fig. 8).According to same step, using sample dispense probe 101a will be dispensed for examination blood plasma to sample container 103d~ 103i.All dispensings for examination blood plasma are repeated until completing (step S109).

In addition, in step S106 and step S108, probe 101a is dispensed to each sample from the sample for forming sample dispensing mechanism 101 The normal plasma of container 103c~103i discharges and the discharge rate for trying blood plasma are set with for example passing through the operation screen shown in Fig. 4 Normal plasma ratio it is corresponding.

Return to Fig. 3, in step S110, as shown in figure 9, using rabbling mechanism 126 will dispense in positioning to rabbling mechanism 126 just Normal plasma in the sample container 103c of lower section for the pooled plasma of examination blood plasma with being stirred.Herein, rabbling mechanism 126 Such as by making rod of the front end provided with paddle or doctor-like infiltrate in the pooled plasma in sample container 103c simultaneously as shown in Figure 9 Rotated to implement to stir.In addition, rabbling mechanism 126 is not limited to the mode for rotating paddle or the rod of doctor-like. For example, also may be configured as, ultrasonic wave is irradiated to the pooled plasma in sample container and is stirred.Adopted in addition, also may replace it With the composition for making sample disk 102 positive and negative (turning clockwise, rotate counterclockwise) rotation or using by normal plasma or for examination blood Starch from sample dispensing probe 101a be expelled to sample container 103 when discharge pressure, i.e. the discharge pressure of sample syringe pump 105 To be stirred the composition of blood plasma.

In this way, in the automatic analysing apparatus 100 of the present embodiment, sample can be utilized according to the operation screen shown in above-mentioned Fig. 4 This dispensing mechanism 101 and rabbling mechanism 126 prepare the pooled plasma with set various normal plasma ratios automatically.

In addition, in Fig. 3, using in dispensing normal plasma (step S106), dispensing supplies to try the knot of blood plasma (step S108) afterwards Structure, but this is not limited to, it can also use and the composition that normal plasma prepares pooled plasma is dispensed after dispensing for examination blood plasma.This Outside, it is contemplated herein that to normal plasma and the viewpoint of the pollution for examination blood plasma is prevented, using separated dispensing normal plasma and for examination blood The composition of slurry, but it is not limited to this.For example, sample dispensing mechanism 101 is cleaned fully, also can be one during without concern of contamination Individual making (preparation) pooled plasma.Now, the desired amount of normal plasma is dispensed to empty sample container 103c, dispenses aequum For examination blood plasma.After the pooled plasma for making sample container 103c is made, pooled plasma is made successively according to each empty sample container, To make empty sample container 103d pooled plasma.

In addition, during 2 kinds of samples of dispensing, it can also be shown at display part 118c in figure 3 at the end of step S109 and prepare knot The picture of beam, and clogged by operator and add the sample container of the sample prepared and be stirred, then place again Onto sample disk 102.Now, without possessing sample rabbling mechanism 126, therefore it is capable of the miniaturization of realization device.

After sample preparation terminates, 7 pooled plasmas prepared on 103c~103i are used to implement analysis.In addition, will be as This measure that then sample preparation is implemented immediately is defined as instant type measure.In Fig. 3 step S111, read using reading part 125 Bar code is taken, the bar code, which is pasted, is housing the sample container 103c of 7 kinds of mutually different pooled plasmas of normal plasma ratio On~103i, only normal plasma, only confession examination blood plasma, 5 kinds of mixing ratios i.e. 10,20,50,80,90% normal plasmas are respectively specified that The pooled plasma of ratio, subsequently into step S112.In step S112, using sample dispensing, mechanism 101 will be housed in each sample Sample in container 103c~103i is dispensed to the mutually different reaction vessel 104 being housed in reaction vessel storage unit 111. Then, each reaction vessel 104 is moved to detection unit 113 using reaction vessel transport mechanism 112, as described above held reaction Device 104 is placed to reaction vessel setting unit 114, and detects the light signal for the intensity for representing scattering light and/or transmitted light.This Place, has following functions:Can be to the normal blood checked in the sample container identification step S103 of 7 samples prepared Ratio and subject sample ID are starched, and is checked with measurement result.

But the difference in cross-mixing experiment with common analysis is, is calculated for subject's sample Multiple (being herein 7) APTT setting times, and a chart is made, for diagnosing.Result after instant type measure terminates shows Be shown as, for example, by such as Figure 10 solid line (a) Suo Shi using the normal plasma ratio of each pooled plasma as transverse axis, with APTT setting times The chart formed, which is drawn, for the longitudinal axis is shown in operating portion 118c (step S113).By using indivedual identification media to measurement result Checked and the automatic drafting function of chart, input error of the operator to measurement result can be prevented, and can carry For the result with reliability.Now, preferably by interface 122 from the print result of printer 123.

After instant type measure, then implement during delaying type measure (step S114), the pooled plasma after instant type is determined The container of (residual samples) clogs, and cultivates certain time at 37 DEG C.Culture is implemented in insulating box 124, but the structure of device When not having insulating box 124 in, implement to cultivate outside device.It is described herein as in order to save the space of device and reduce cost, Insulating box 124, and situation about being cultivated outside device are not set in a device.In device, to determine the end time as starting point, Count incubation time.Now, operator is by presetting incubation time, at the end of culture can be known from operation screen Carve.Furthermore it is preferred that the display (step S115) that output notice culture terminates when close to culture finish time.Therefore, operator The situation of the sample in culture can be held, will not occur to forget measure.Operator is opened the pooled plasma for cultivating end Bolt, and place to sample disk 102, press measure start button.Press measure start button after (step S116), with sample disk 102 rotation, read the pooled plasma ID for being arranged on the position specified in the operation screen shown in Fig. 5, and identification step S103 The middle normal plasma ratio checked and subject sample ID, are checked (step S117) with authorized item, implement delaying type Determine (step S118).After measure terminates, the chart (step S119) of the delaying type shown in Figure 10 solid line (b) is made, and with The result of instant type is checked (step S120).At this point it is possible to the chart of instant type and delaying type is synthesized one as shown in Figure 2 Zhang Tu, or can also shown separately mutually different 2 charts.

As it appears from the above, according to the present embodiment, making, the analysis of instant type/delaying type of pooled plasma can be implemented automatically And the result verification of instant type/delaying type, therefore be not in the measurement result caused by the proficiency difference of operator Deviation and artificially take wrong sample etc. slip up, can further improve reliability.

Additionally it is possible to mitigate the burden of operator, result is obtained rapidly.

Furthermore it is possible to realizing can make to realize for examination blood plasma and the pooled plasma that normal plasma mixes with defined mixing ratio Automatically the automatic analysing apparatus and automatic analysis method prepared.

【Embodiment 2】

Figure 11 is the overall schematic configuration of the automatic analysing apparatus of the embodiment 2 involved by the other embodiment of the present invention Figure.In the present embodiment, automatic analysing apparatus possess sample rack 201, sample rack supply unit 202, sample rack incorporating section 203, by sample Transport to the carrying line 204 of analysis portion 210, the line of return 205, sample rack standby unit 206, standby unit operating mechanism for this 201 207th, sample rack gigback 208, the 1st reading part (carrying line) 209 and analysis portion 210.It is used for carrying that is, being used as The mechanism of sample container 103, have and sample container 103 is mounted on sample rack 201 and transports the various conveyances of sample rack 201 This point of mechanism is different from embodiment 1.Other aspects are same as Example 1, therefore for inscape mark same as Example 1 Note same label, explanation omitted below.

As shown in figure 11, multiple analysis portions 210 can be connected along carrying line 204 by consisting of, but in the present embodiment, is configured to Possesses at least one analysis portion for being used for implementing measuring clotting time.For implement the basic composition of the analysis portion 210 of measuring clotting time with And fundamental analysis flow is roughly the same with embodiment 1, but sample is supplied by carrying line 204, therefore does not have sample disk 102。

Described further below and embodiment 1 has the supply method of the sample of larger difference.

In the automatic analysing apparatus of the present embodiment, the conveyer of the analysis portion 210 configured along carrying line 204 possesses:2nd reads Portion's (analysis portion) 211, it is used for the analysis entrusted information for checking corresponding sample;1st sample rack operating mechanism 212, it is from conveyance Line 204 receives sample rack 201;Line 213 is dispensed, it possesses the function that sample rack 201 can be made standby to dispensing start, and by sample In this dispensing to the sample container of sample rack 201;Buffer region 214, it makes sample when preparing cross-mixing pooled plasma Frame 201 is kept out of the way;And the 2nd sample rack operating mechanism 215, its sample rack 201 after sample is dispensed are transported to the line of return 205.

First, illustrate to implement sample when in general analyzes the i.e. analysis of calibration object, management product, general sample etc. using Figure 12 This supply flow is the conveyance order of sample rack.

When accepting analysis commission by operating portion 118, sample rack 201 on sample rack supply unit 202 is arranged in fig. 12, such as After transferring load to carrying line 204 shown in arrow (a), read by the 1st reading part (carrying line) 209 and paste in sample rack 201 and receive Hold to the individual identification media (such as bar code etc.) in the sample container in sample rack 201, and identify sample rack number and sample This bin number (arrow (b) in Figure 12).Then, if having sample rack 201 in dispensing line 213, the 1st reading part is passed through The sample that (carrying line) 209 is read, which can house, to sample rack standby unit 206, to be waited (arrow (c) in Figure 12) to be analyzed.Dispensing The sample rack 201 that the stage of the sample dispensing end of line 213 is standby can be sent to analysis portion 210, pass through (the analysis of the 2nd reading part Portion) 211 identification sample rack numbers and sample container number ((d) in Figure 12).Then, the 1st sample rack operating mechanism is passed through 212, dispensing line 213 ((e) in Figure 12) is drawn into, dispensing mechanism 101 by sample dispenses sample.Now, if dispensing line There is no sample rack 201 on 213, then will not house to sample rack standby unit 206, but directly conveyance extremely dispensing line 213.

House sample dispensing mechanism 101 dispensing terminate after sample sample rack 201 can by the 2nd sample rack operation machine Structure 215 is transported to the line of return 205 ((f) in Figure 12), and is transported by standby unit operating mechanism 207 to sample rack standby unit 206 ((g) in Figure 12).Herein, measurement result is waited, when being judged to not checking again for, passes through standby unit operating mechanism 207 transfer load to the line of return 205 ((h) in Figure 12), and transport to sample rack incorporating section 203 (Figure 12 (i)).

Figure 13 show cross-mixing experiment sample prepare in sample-feed flow, i.e. sample rack conveyance sequentially.

After accepting analysis commission by operating portion 118, the sample rack 201 that is arranged on sample rack supply unit 202 is removed transferring load to After line sending 204 (arrow (a) in Figure 13), read by the 1st reading part (carrying line) 209 and paste in sample rack 201 and house Individual identification media (such as bar code) in sample container in sample rack 201, identify sample rack number and sample container Number ((b) in Figure 13)., can be to housing when confirming the commission that cross-mixing is tested by the 1st reading part (carrying line) 209 Normal plasma, the progress complete check of sample rack 201 for trying blood plasma and empty sample container, and housed to sample rack and treated Machine portion 206 etc. is to be analyzed, until not having the sample (arrow (c) in Figure 13) in analysis on dispensing line 213.Now, house and supply The sample rack of examination blood plasma, normal plasma and empty sample container can be all housed in same sample rack, can also cross over two Individual sample above frame is housed.

There is no the sample in analysis on dispensing line 213 and house analysis object i.e. for examination blood plasma, normal plasma and empty sample When the sample rack 201 of this container is firmly established, according to the sample rack for housing the confession examination blood plasma as analysis object, house The order of the sample rack of empty sample container, transport to analysis portion 210.Then, sample is identified by the 2nd reading part (analysis portion) 211 This number and sample container number ((d) in Figure 13).Then, dispensing line is delivered to by the 2nd sample rack operating mechanism 215 213 ((e) in Figure 13), in the same manner as above-described embodiment 1, using under the liquid level detection function of sample dispensing mechanism 101, exception Drop detection function, confirmation had been filled with it is the desired amount of for examination blood plasma, normal plasma and for prepare pooled plasma institute it is required Sample container 103 for sky.

Herein, when setting incorrect for examination plasma volume, normal plasma amount and empty sample container, machine is operated by the 1st sample rack After structure 212 is returning briefly to carrying line 204, by the 2nd sample rack operating mechanism 215, it can transport to the line of return 205, and return to sample Behind this incorporating section 203, output system is alarmed and stops sample and prepares.

On the other hand, it is confirmed that for examination plasma volume, normal plasma amount and empty sample container, correct sample rack 201 is set By the 1st sample rack operating mechanism 212 returning briefly to carrying line 204, and transported again by the 2nd sample rack operating mechanism 215 To dispensing line 213.The transported sample rack 201 of suction supplies examination blood plasma, then, is expelled to sky sample container.Now, empty sample When container is housed on other sample racks, make sample rack configuration in the buffer region 214 on dispensing line 213, and pass through sample The arc-shaped spinning movement of this dispensing mechanism 101 in the horizontal plane, is expelled to sky sample container (Figure 14).Repeat same move Make, pooled plasma is made for examination blood plasma and normal plasma in dispensing.Now, the 126 preferred structure of rabbling mechanism shown in embodiment 1 Turn into:It is configured to be accessed on dispensing line 213, and the mixing of pooled plasma can be implemented.

The sample rack for housing the sample prepared returns to carrying line 204 by the 1st sample rack operating mechanism 212, and The line of return 205 ((g) in Figure 13) is delivered to by the 2nd sample rack operating mechanism 215.Thereafter, the sample prepared is housed Sample rack 201 by standby unit operating mechanism 207, be drawn into sample rack standby unit 206 ((h) in Figure 13), wait for Analysis.Herein, as shown in figure 11, when analysis portion 210 does not have the construction of rabbling mechanism 126, machine is operated by the 2nd sample rack Structure 215 delivers to the line of return 205 ((g) in Figure 13), and returns to sample rack incorporating section 203 ((g) in Figure 13).Operator is from returning The sample prepared is reclaimed on the sample rack returned, and is placed to again on sample rack supply unit 202 after agitation, is implemented any Project (such as APTT) analysis (measure of instant type).Due to analysis method similarly to Example 1, therefore omit the description.

The sample that analysis terminates delivers to the line of return 205 ((g) in Figure 13) by the 2nd sample rack operating mechanism 215, and returns Return sample rack incorporating section 203 ((g) in Figure 13).Operator reclaims the sample for being back to sample rack incorporating section 203, at 37 DEG C Implement culture certain time, and be placed to again on sample rack supply unit 202, implement the measure of delaying type.

According to the present embodiment, in addition to the effect of embodiment 1, additionally it is possible to by controlling the conveyance direction of sample rack, hold Changing places, measure and delay determine immediately for implementation.

【Embodiment 3】

Figure 15 is the handling process for the automatic analysing apparatus for showing the embodiment 3 involved by the other embodiment of the present invention Flow chart.In the present embodiment, the composition of automatic analysing apparatus itself can be using a certain in above-described embodiment 1 or embodiment 2 Individual composition, it replaces sample (making pooled plasma) that measure object is prepared in sample container, but by normal plasma and It is different from embodiment 1 and embodiment 2 that this point is directly dispensed to the preparation of implementation measure object sample in reaction vessel for examination blood plasma. That is, in the present embodiment, by adding examination again after directly being dispensed by normal plasma and for examination blood plasma to reaction vessel Agent is implemented to determine, therefore with as shown in above-mentioned embodiment 1 and embodiment 2, utilizing other container mixing normal plasmas and for examination Blood plasma, and after pooled plasma is made, in measure dispensing the method into reaction vessel again compares, the loss of sample compared with It is few.

As shown in figure 15, first, automatic analysing apparatus accepts the commission (step of cross-mixing experiment by operating portion 118 S301).Then, in the same manner as above-described embodiment 1 and embodiment 2, (walked identifying that the START button shown in Fig. 5 is pressed Rapid S302) before, it is same as Example 1.

As shown in figure 16, in the cross-mixing measure commission picture of the present embodiment, similarly to Example 1, by operator To specify the selection of analysis project that normal plasma ratio set, inspection project select/carried out by designated area 127 to specify. Herein, as shown in figure 16, in the present embodiment in addition to subject ID, normal blood can be inputted by being provided with also on operation screen Starch the region of ID and the position for examination blood plasma/normal plasma.After pressing START button, pasting for examination blood plasma/normal blood , can be with the rotation of sample disk 102, before by reading part 125 in the case of the individual identification media such as bar code of slurry It is identified for the sample ID for trying blood plasma/normal plasma.When not pasting when sample ID reads failure or individual identification media, energy Enough by supplying in the sample ID of examination blood plasma/normal plasma and the column of position to be manually entered to enter on Figure 16 operation screen Row identification.In addition, when accepting commission by using the network system of master computer, measure project and measure bar can need not be set Analyzed part.

After pressing start button (step S302), sample dispensing mechanism 101 can aspirate be arranged on it is normal on sample disk 102 Blood plasma, and dispense to reaction vessel 104 (step S303).Then suction is for examination blood plasma, and dispenses to (the step of reaction vessel 104 S304).Herein, it is shown that according to normal plasma, for try blood plasma order dispensed the step of, but these steps S303 and S304 order may be reversed.In addition, after suction normal plasma, reaction vessel 104 can not also be expelled to, directly suction supplies Blood plasma is tried, and concentrates and is expelled to reaction vessel 104.

If the measure is instant type measure (step S305), reaction vessel transport mechanism 112 can grip reaction vessel, And it is moved to detection unit 113 (step S306).Then, reagent dispensing (step S307) is implemented using reagent dispensing mechanism 106 And examinations (step S308), it is repeated up to all samples of the normal plasma ratio set in the operation screen shown in Figure 16 Measure (S309) is completed, the chart (step S310) of instant type measurement result is drawn after the completion of measure.

, will when the measure of the reaction vessel is not instant type measure, when being delaying type measure in addition, in step S305 Reaction vessel 104 is moved to insulating box 124 (step S311), and stores heating start-up time.Repeat the operation picture shown in Figure 16 The preparation (step S312) of all pooled plasmas of the normal plasma ratio set in face, cultivates all pooled plasmas.Reaching Heat the stage (step S313) of end time, reaction vessel transport mechanism 112 can grip reaction vessel 104, and reaction is held Device 104 is moved to detection unit 113 (step S314).In the present embodiment, incubation time can be managed in automatic analysing apparatus, Therefore can mitigate because of the deficiency of incubation time or due to exceeding and the risk of output error result.Herein, can be in operation screen The middle display time, to know that cross-mixing experiment is implemented in culture.More preferably with when can flexibly set culture Between function.Then, reagent dispensing (step S315), and examinations (step S316) are implemented using reagent dispensing mechanism 106. All samples completion measure (step S317) until being prepared is repeated, delaying type measurement result is drawn after the completion of measure Chart (step S318).

Then, in step S319, by the chart of the instant measurement result obtained by step S310 and step is passed through The chart for the delaying type measurement result that S318 is obtained is checked, and is set to final result (step S319).

In this way, according to the present embodiment, by directly being dispensed to reaction vessel 104, and entirely by normal plasma and for examination blood plasma The output of the preparation of pooled plasma, culture, measure and measurement result is automatically carried out, can be independent of the proficiency of operator Ground provides the result of high reliability.Additionally it is possible to mitigate the negative of subject by reducing the sample size used in preparation Load.

In addition, in the present embodiment, it is configured to can also to directly input and is filled with normal plasma and the sample container for examination blood plasma Position, therefore be also applied in using without using the facility of sample ID management functions.

【Embodiment 4】

Figure 17 represents the handling process of the automatic analysing apparatus of the embodiment 4 involved by the other embodiment of the present invention. The implementation of cross-mixing experiment is illustrated in 1~embodiment of embodiment 3, but automatic analysing apparatus not is only implemented to intersect to mix Experiment is closed, is generally also used in solidify the ill assurance of fibrinolytic system, DIC (disseminated intravascular coagulation) diagnosis, thrombus In inspection for the purpose of confirmation of therapeutic effect etc..That is, cross-mixing inspection pooled plasma complete culture and it is real When applying delay type analysis, if being mixed with common analysis commission, the mixing blood of cross-mixing can be not necessarily analyzed immediately Slurry.Therefore, in the automatic analysing apparatus of the present embodiment, with can be according to the work(of the priority of the categorizing selection inspection of sample Can aspect and the difference of 1~embodiment of embodiment 3.

In the automatic analysing apparatus illustrated in the present embodiment, for corresponding blood clotting energy preoperative in clinical examination Require swiftness when same day report of inspection result etc. is provided during the inspection of power or to outpatient, having can be prior to logical Normal sample implements the function of analysis.Herein, it is desirable to which these swiftness samples, which are referred to as, is defined as urgent sample, and having can be excellent Prior to the feature that general sample implements analysis.On the other hand, trained due to being managed in the pooled plasma for cross-mixing inspection The foster time, it is expected to be measured immediately when terminating the culture of certain time so requiring.Therefore, energy is illustrated using Figure 17 Enough demands according to operator select priority according to sample classification, and the processing of measure order is determined according to priority.Figure 17 In, in case of by priority level initializing for " urgent sample measures > delaying types cross-mixing tests the general sample measures of > " Illustrate, but the setting of priority is not limited to this mode.

For the pooled plasma made for delaying type cross-mixing culture at the end of (step S401), device is not Can be according to below scheme (step S402) during holding state.Commission (the step of urgent sample is whether there is in the project of decision plan Enter step S406 when S403), without the commission of urgent sample, re-start scheduling so that compared with the commission of general sample, It is preferential to implement delaying type cross-mixing experiment (step S406).But when having entrusted the analysis of urgent sample, it is first according to urgent Sample, cross-mixing experiment, the order of general sample implement analysis, therefore in the analysis of urgent sample, can make cross-mixing Pooled plasma is temporarily standby in insulating box 124 (step S404).When the analysis of urgent sample is completed (step S405), weight Newly it is scheduled so that preferential to implement delaying type cross-mixing experiment (step S406) compared with the commission of general sample.This Place, for the pooled plasma made for delaying type cross-mixing culture at the end of (step S401), if device is in Holding state is discussed further below starting the analysis of cross-mixing experiment then without being scheduled as described above.

First, make to be moved to detection unit 113 from insulating box 124 for the pooled plasma that delaying type cross-mixing makes (step S407).Then, reagent (step S408), and examinations (step S409) are dispensed to the pooled plasma prepared.Weight Multiple implementation steps S407~step S409 processing, until owning for the pooled plasma prepared for delaying type cross-mixing Analysis terminates.After the completion of the analysis of pooled plasma (step S410), the result (step of simultaneously output cross bulk testing is calculated S411)。

Then, it is surplus have the commission of general sample when (step S412), analyze general sample (step S413), general sample Analysis after the completion of (step S414), automatic analysing apparatus can turn into holding state (step S415).

Figure 18 is the timing diagram for the action for showing the automatic analysing apparatus shown in Figure 17.As shown in figure 18, automatically analyzing When device itself is holding state, after accepting the measure commission of general sample, automatic analysing apparatus, which can turn into, to be used for determining typically The running status of sample.Now, it is assumed that, then can be in advance as described above by urgent sample after accepting the measure commission of urgent sample The priority level initializing of measure processing is highest, is determined followed by cross-mixing experiment with the delaying type of sample (pooled plasma), one As sample measure priority level initializing to be minimum.Therefore, urgent sample process can preferentially be implemented.Urgent sample process is completed Afterwards, start again in general sample process, if the incubation time of cross-mixing experiment pooled plasma terminates, can temporarily in The processing of disconnected general sample, and start delaying type measure.Implement in delaying type measure, accepted the measure commission of urgent sample When, even if the priority level initializing of urgent sample process is higher, urgent sample process also can turn into holding state until delaying type Measure terminates, and after cross-mixing off-test, implements the analysis of urgent sample.

In this way, according to the present embodiment, in addition to the effect of embodiment 1 and embodiment 2, by for general sample, tight Insertion process of anxious sample etc., priority is set to each sample by operator in advance, can be based on automatic analysing apparatus set Fixed priority implements analysis, reduces sample and takes the human errors such as mistake, and effectively runs automatic analysing apparatus.

【Embodiment 5】

Figure 19 is the handling process for the automatic analysing apparatus for showing the embodiment 5 involved by the other embodiment of the present invention Flow chart.In the present embodiment, this point of Reagent management and above-described embodiment 1 to embodiment 4 are implemented based on pooled plasma sample number It is different.In addition, the composition and embodiment 1 beyond reagent management method are same to embodiment 4, therefore its explanation omitted below.

In cross-mixing experiment, such as handled 7 measured values as one group of result, therefore for one group of measure, it is necessary to Ensure its reagent for same batch (being preferably same bottle).When especially measure project is APTT, due to not implementing to calibrate, institute During with the different reagent of batch, deviation is easily produced in measurement result.In addition, even same batch, is saved in device The reagent of reagent container (reagent bottle) of the reagent with newly breaking a seal in the reagent container (reagent bottle) of a period of time also can be produced easily Raw deviation.Herein, in the automatic analysing apparatus of the present embodiment, after the analysis commission for confirming cross-mixing experiment, it is necessary to ensure that energy Enough reagents at least implementing one group of measure, this puts critically important.As shown in figure 19, (walked after accepting the analysis commission of instant type Rapid S501), control unit 120 can confirm necessary test number (the pooled plasma number being configured to) and reagent residual.That is, sentence The fixed relation (step S502) for whether meeting " the remaining test number of pooled plasma sample number≤reagent ".Step S502 result of determination When exceeding reagent residue test number for "No", i.e. pooled plasma sample number, into step S504, and show and report in display part 118c It is alert.In addition, when the result of step S502 judgement is tested below number for pooled plasma sample number for reagent is remaining, into step S503, and implement cross-mixing experiment (implementing analysis).

In addition, control unit 120 can be implemented to control when being provided with the reagent bottle of multiple same projects so that at least at one group Will not across bottle implementation analysis in measure.For example, the remaining test number of bottle 1 is " 100 times for the remaining test of " 3 tests ", bottle 2 Test " and cross-mixing experiment for 7 points (7 kinds of conditions) commission when, " reagent of pooled plasma sample number (7) >=bottle 1 is remaining Test number ", therefore cancel the analysis of bottle 1, checked with the remaining test number of bottle 2.During bottle 2, due to " pooled plasma sample number The remaining test number of the reagent of (7)≤bottle 2 ", so cross-mixing experiment (analysis) can be implemented.Moreover, it is assumed that neither one can During the reagent bottle analyzed, meeting output system, which is alarmed and cancels analysis, to be started (step S504).

According to the present embodiment, when implementing one group of cross-mixing experiment, reagent deficiency will not occur in analysis midway, can make Analyzed with the reagent of same bottle.Therefore, it is possible to provide the result of high reliability.

【Embodiment 6】

Figure 20 is the handling process for the automatic analysing apparatus for representing the embodiment 6 involved by the other embodiment of the present invention Flow chart.In the present embodiment, the compound method of pooled plasma is different from 1~embodiment of above-described embodiment 5.Other, automatic point The composition of analysis apparatus, the flow of general measuring clotting time are identical with embodiment 1 or embodiment 2, therefore repeat specification omitted below.This Outside, the compound method on sample, part same as Example 1 can simplify explanation as far as possible.

In above-described embodiment 1 and embodiment 2, consist of, as shown in figure 5, needed for being shown in display part 118c just Normal plasma volume and to operator avoid that the risk of sample deficiency occurs in midway for examination plasma volume, and by notice.But ten thousand One because operator error occur normal plasma amount and/or for examination plasma volume deficiency when, can preparation midway occur sample not Foot, and the sample prepared can be wasted.Therefore, in the automatic analysing apparatus of the present embodiment, even in should prepare as described above Normal plasma and/or for examination blood plasma occur it is insufficient when, will not also waste sample, can effectively implement to determine.

As shown in figure 20, the commission (step S601) of cross-mixing experiment is accepted in automatic analysing apparatus, and sets measure After project and normal plasma ratio, analysis operation control part 120a can implement following handle.That is, analysis operation control part 120a can calculate the normal plasma amount needed for measure and supply examination plasma volume, determine mutually different normal plasma respectively according to condition Measure and for examination plasma volume, and control the action of sample dispensing mechanism 101.

Then, after pressing the START button on the operation screen shown in Fig. 5, analysis operation control part 120a will recognise that this START button has been pressed (step S602).Then, confirm whether there is sky sample container (step S603).Whether there is sky sample container Confirmation method it is identical with Fig. 3 step S103 processing (embodiment 1).

In step S604, whether the empty sample container number for judging to obtain by implementation steps S603 is more than N (N is natural number). In addition, N is set as empty sample container for example corresponding with the normal plasma ratio set on the operation screen shown in Fig. 5 herein Number i.e. 7.The result of judgement is when the empty sample container of required number is not provided with defined position, to stop sample and prepare, and (step S605) is alarmed in display part 118c display systems.On the other hand, the result of judgement is to set to have time on defined position During sample container, into step S606, the liquid level detection function that mechanism 101 is dispensed using sample checks plasma volume.

But the measure number in cross-mixing experiment is recommended to be at least 3.In other words, cross-mixing experiment is more than 3 When, it can implement.Figure 21 represents the display example of the operation screen when commission cross-mixing of the present embodiment is tested.Such as Figure 21 institutes Show that in cross-mixing tests measure commission picture, there is the region for the priority that can select normal plasma ratio.Figure 21 institutes In the example shown, it can divide for 3 stages inputted successively from high to low according to priority.That is, in priority level initializing region The priority of middle setting meets the relation of " the > priority 3s of 1 > priority of priority 2 ".Shown here as by normal plasma ratio 0%th, 50% and 100% is set as priority 1, and normal plasma ratio 10% and 20% are set as into priority 2, will be normal Plasma ratio 80% and 90% state for being set as priority 3.The priority of these settings can be stored in storage part 119.

In addition, Figure 22 to Figure 24 shows normal plasma amount corresponding with each normal plasma ratio and the relation for examination plasma volume.Such as Shown in Figure 22, according to 7 kinds of conditions that normal plasma ratio is 0,10,20,50,80,90,100%, the mixed of 200 μ L is made respectively Close blood plasma, implement cross-mixing experiment when, normal plasma, for examination blood plasma be respectively necessary for more than 700 μ L.Herein, even if illustrating it One or both of when not meeting aequum, the method that can also utilize less plasma volume to obtain effective analysis result.

Herein, Figure 20 is returned, in step S607, judges whether normal plasma amount is XNIt is above and for examination plasma volume No is YNMore than.Herein, XNIt is 700 μ L, Y in the example shown in Figure 22NIt is similarly 700 μ L.The result of judgement is " normal blood Slurry amount >=XN" and " for examination plasma volume >=YN" in some or two do not meet during condition, i.e. plasma volume does not meet aequum When, step S608 can be entered.

In step S608, for change measure points, with reference to be stored in storage part 119 to the excellent of each normal plasma ratio set First level, plasma volume will be calculated again except condition corresponding with priority 3.In step S609, determine whether to meet " normal blood Slurry amount >=(XN-XP3) " and " for examination plasma volume >=(YN-YP3)”.Herein, when being determined under conditions of except by priority 3 just Normal plasma volume (XN-XP3) it is 360 μ L, supply examination plasma volume (Y when being determined under conditions of except by priority 3N-YP3) it is 640 μ L (Figure 23).The result of judgement is " normal plasma amount >=(XN-XP3) " and " for examination plasma volume >=(YN-YP3) " in some or Two when not meeting condition, into step S610.On the other hand, result of determination is when meeting above-mentioned condition, into step S613.

In step S610, except in the only condition of setting priority 1, i.e. by priority 2 and the condition of priority 3 Under the conditions of determine when plasma volume recalculated.Herein, the μ L of normal plasma amount 300 and confession examination plasma volume 300 μ L can conducts Plasma volume after recalculating obtains (Figure 24).

Then, into step S611, determine whether to meet " normal plasma amount >=(XN-XP3-XP2) " and " for examination plasma volume >=(YN- YP3-YP2)”.Result of determination is normal plasma amount >=(XN-XP3-XP2) " and " for examination plasma volume >=(YN-YP3-YP2) " in it is a certain It is individual or when two do not meet condition, into step S612, alarmed to display part 118c output systems, and stop matching somebody with somebody for pooled plasma System.On the other hand, result of determination is when meeting above-mentioned condition, into step S613.

In step S613, analysis operation control part 120a can control sample dispensing mechanism 101 and reagent dispensing mechanism 106, And start normal plasma and the dispensing for examination blood plasma.In addition, normal plasma and dispensing and above-described embodiment 1 for trying blood plasma are same Sample, therefore omit the description herein.After the dispensing of all normal plasmas and confession examination blood plasma completes (step S614), action control is analyzed Portion 120a can control rabbling mechanism 126, implement the stirring (step S615) of pooled plasma.After pooled plasma stirring, it can implement point Analysis.

In Figure 20, when being 3 stages by priority level initializing exemplified by be illustrated, but be not limited to this, priority can Any setting.

According to the present embodiment, in addition to the effect of above-described embodiment 1 and embodiment 2, even if not meeting with setting originally Normal plasma ratio corresponding to measure needed for normal plasma amount and/or for examination plasma volume when, can also obtain effective friendship Pitch mixed determining result.

In addition, the present invention is not limited to above-described embodiment, various modifications example is included.For example, in order in the explanation present invention When be understood that, be described in detail by above-described embodiment, but be not limited to possess illustrated all structures.This Outside, the part-structure of a certain embodiment can be replaced with to the structure of other embodiment, can also be by the structure of a certain embodiment Added in the structure of other embodiment.Further, it is also possible to the part-structure of each embodiment is deleted or adds, replace it The structure of his embodiment.

Label declaration

100 automatic analysing apparatus

101 samples dispense mechanism

101a samples dispense probe

102 sample disks

103 sample containers

104 reaction vessels

105 sample syringe pumps

106 reagents dispense mechanism

106a reagents dispense probe

107 reagent discs

108 reagent containers

108a reagents

109 reagent heating-up mechanisms

110 reagent syringe pumps

111 reaction vessel storage units

112 reaction vessel transport mechanisms

113 detection units

114 reaction vessel setting units

115 light sources

116 test sections (light accepting part)

117 reaction vessel waste parts

118 operating portions

118a mouses

118b keyboards

118c display parts

119 storage parts

120 control units

120a analyzes operation control part

120b operational parts

121 A/D converters

122 interfaces

123 printers

124 insulating boxs

125 reading parts

126 rabbling mechanisms

127 inspection project selections/designated area

201 sample racks

202 sample rack supply units

203 sample rack incorporating sections

204 carrying lines

205 lines of return

206 sample rack standby unit

207 standby unit operating mechanisms

208 sample rack gigbacks

209 the 1st reading parts (carrying line)

210 analysis portions

211 the 2nd reading parts (analysis portion)

212 the 1st sample rack operating mechanisms

213 dispensing lines

214 buffer regions

215 the 2nd sample rack operating mechanisms.

Claims (20)

1. a kind of automatic analysing apparatus, it is characterised in that possess:
Sample dispenses mechanism, and it is by for examination blood plasma and/or to correct the normal blood that the setting time for trying blood plasma is added Slurry is dispensed to multiple sample containers;
Reaction vessel, it prepares only described for examination blood plasma, only described normal plasma and with least one kind of in the sample container The pooled plasma mixed for trying blood plasma and normal plasma is utilized dispensing institute of sample dispensing mechanism by mixing ratio State confession examination blood plasma, the normal plasma and the pooled plasma being formulated;
Reagent dispenses mechanism, and it dispenses reagent to the reaction vessel;And
Determination part, it in the reaction vessel to the addition of the described for examination blood plasma, normal plasma and/or pooled plasma of reagent Irradiate the light from light source, and the scattering light based on acquisition and/or transmission light measurement setting time.
2. automatic analysing apparatus according to claim 1, it is characterised in that
With sample disk, it is annular in shape and houses separated from each other described for examination blood plasma, the normal plasma and the mixing Blood plasma.
3. automatic analysing apparatus according to claim 1, it is characterised in that possess:
Sample rack, it is described for examination blood plasma, the normal plasma and the pooled plasma for housing;
Sample rack supply unit, it is used for housing multiple sample racks;
Carrying line, it is used for from the sample rack supply unit transporting the sample rack to the determination part;And
The line of return, it transports the sample rack to sample rack incorporating section from the determination part.
4. automatic analysing apparatus according to claim 1, it is characterised in that
With display part, it, for examination blood plasma, the normal plasma and the pooled plasma, is shown for described with graph mode The setting time obtained by determination part.
5. automatic analysing apparatus according to claim 4, it is characterised in that
Possesses insulating box, each reaction vessel for housing the pooled plasma is heated the stipulated time by it with set point of temperature.
6. automatic analysing apparatus according to claim 5, it is characterised in that
Reagent is dispensed to being housed in each reaction vessel and after heating stipulated time using reagent dispensing mechanism It is described in examination blood plasma, the normal plasma and the pooled plasma, and utilize determination part measure setting time.
7. automatic analysing apparatus according to claim 4, it is characterised in that
House be configured to by the sample dispensing machine it is only described for examination blood plasma, only described normal plasma and with described In the differential responses container for the pooled plasma that at least one kind of mixing ratio is formulated, reagent dispensing mechanism dispensing is utilized Reagent, and utilize determination part measure setting time.
8. automatic analysing apparatus according to claim 4, it is characterised in that
For described for examination blood plasma, the normal plasma and the pooled plasma, supplied using described in indivedual identification media managements Try the information of blood plasma, the normal plasma and the pooled plasma.
9. automatic analysing apparatus according to claim 4, it is characterised in that
With level detection mechanism, it confirms for examination plasma volume, normal plasma amount and empty sample container in advance.
10. automatic analysing apparatus according to claim 9, it is characterised in that
For only described for trying blood plasma, only described normal plasma and the pooled plasma with different multiple mixing ratios, in advance Set priority,
When predicting that deficiency occurs in the confession examination plasma volume and/or the normal plasma amount, based on the priority of the setting, determine Surely the combination for examination blood plasma, the normal plasma and the pooled plasma of the setting time should be determined.
11. automatic analysing apparatus according to claim 4, it is characterised in that
To the residual of each reagent container management measure object reagent, and the examination used according to the control of the quantity of the pooled plasma Agent container.
12. automatic analysing apparatus according to claim 4, it is characterised in that
The determination part preferentially determines swiftness high sample.
13. automatic analysing apparatus according to claim 12, it is characterised in that
With operating portion, it is in advance to urgent sample, general sample and containing for determining the described mixed of the setting time Close the sample setting priority of blood plasma.
14. the automatic analysing apparatus according to claim 10 or 13, it is characterised in that have:
Storage part, it stores each priority;And
Control unit, it implements control so that, will by sample disk or carrying line based on the priority being stored in the storage part Corresponding sample is transported to the determination part.
15. a kind of automatic analysis method, it is that at least there is sample to dispense the automatic of mechanism, reagent dispensing mechanism and determination part The automatic analysis method of analytical equipment, it is characterised in that
It will be added just for examination blood plasma and/or to correct the setting time for trying blood plasma using sample dispensing mechanism Normal blood plasma is dispensed to multiple sample containers,
Prepared in the sample container it is only described for examination blood plasma, only described normal plasma and with least one kind of mixing ratio by institute The pooled plasma mixed for examination blood plasma and normal plasma is stated,
By it is described make it is described housed for examination blood plasma, the normal plasma and the pooled plasma to reaction vessel, together Reagent dispensing mechanism dispenses reagent to the reaction vessel described in Shi Liyong,
The described of reagent that with the addition of in the reaction vessel is come from for examination blood plasma, normal plasma and/or pooled plasma irradiation The light of light source, and the scattering light based on acquisition and/or transmission light measurement setting time.
16. automatic analysis method according to claim 15, it is characterised in that
By for each setting time drafting pattern obtained for examination blood plasma, the normal plasma and the pooled plasma Table, and show to display part.
17. automatic analysis method according to claim 16, it is characterised in that
Each reaction vessel for examination blood plasma, the normal plasma and the pooled plasma will be housed at an established temperature Heat the stipulated time,
Reagent is dispensed into the reaction vessel after the heating stipulated time, determines setting time.
18. automatic analysis method according to claim 16, it is characterised in that
For only described for trying blood plasma, only described normal plasma and the pooled plasma with different multiple mixing ratios, in advance Set priority,
Based on the priority of the setting, determine to determine the setting time it is described for examination blood plasma, the normal plasma with And the combination of the pooled plasma.
19. automatic analysis method according to claim 16, it is characterised in that
In advance to urgent sample, general sample and containing being set for determining the sample of the pooled plasma of the setting time Determine priority,
The order analyzed according to the priority change of the setting.
20. a kind of automatic analysing apparatus, it is characterised in that possess:
Multiple sample containers, it is housed only for trying blood plasma, only normal plasma and supplying to try by described with least one kind of mixing ratio respectively The pooled plasma that blood plasma and normal plasma mix;
Individual identification media, it is attached in each sample container, and storage is used for identifying the sample housed in the sample container Information;
Reading part, it reads the information being stored in the individual identification media;
Sample dispenses mechanism, and it will be only described for trying blood plasma, only normal plasma and pooled plasma respectively from the multiple sample Container is dispensed into reaction vessel;
Reagent dispenses mechanism, and it dispenses reagent to the reaction vessel;
Determination part, the described of reagent that with the addition of in the reaction vessel is shone for examination blood plasma, normal plasma and pooled plasma Penetrate the light from light source, and the scattering light based on acquisition and/or transmission light measurement setting time;
Operational part, it is based on obtaining by the determination part described for examination blood plasma, just after the pooled plasma is just mixed The measurement result of normal blood plasma and pooled plasma, draws the instant type figure for showing the mixing ratio and the relation of the setting time Table, and in the mixing from the pooled plasma after the stipulated time, it is described for trying based on being obtained by the determination part The measurement result of blood plasma, normal plasma and pooled plasma, draw and show the mixing ratio and the relation of the setting time Delaying type chart;And
Display part, it is overlapping to show the described instant of same sample group based on the information being stored in indivedual identification media The chart of type and the delaying type.
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Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2019028019A (en) * 2017-08-03 2019-02-21 株式会社島津製作所 Pretreatment device and analysis system equipped with pretreatment device
CN107469705A (en) * 2017-08-28 2017-12-15 王保民 A kind of Novel test section is mixed and shakes device automatically with blood

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009008552A (en) * 2007-06-28 2009-01-15 Hitachi High-Technologies Corp Automatic analysis apparatus and dispensing method for automatic analysis apparatus
CN102066947A (en) * 2008-06-18 2011-05-18 积水医疗株式会社 Method for determining factor causing the prolongation of blood coagulation time
CN103688177A (en) * 2011-06-17 2014-03-26 学校法人东日本学园 Method of measuring blood coagulation time to detect lupus anticoagulants
CN104350386A (en) * 2012-06-11 2015-02-11 株式会社日立高新技术 Automatic analysis apparatus

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS635268A (en) * 1986-06-25 1988-01-11 Shimadzu Corp Multi-item automatic analyser
JP2001249137A (en) * 2001-02-19 2001-09-14 Olympus Optical Co Ltd Autoanalyzer
JP2009204446A (en) * 2008-02-28 2009-09-10 Hitachi High-Technologies Corp Automatic analyzer
JP4729594B2 (en) * 2008-04-28 2011-07-20 株式会社日立ハイテクノロジーズ Automatic analyzer
WO2010087137A1 (en) * 2009-01-29 2010-08-05 株式会社 日立ハイテクノロジーズ Autoanalyzer
JP5618489B2 (en) * 2009-02-17 2014-11-05 シスメックス株式会社 Analysis device, analysis method, and computer program
CN103748472B (en) * 2011-09-05 2016-08-17 株式会社日立高新技术 Automatic analysing apparatus
JP2014190954A (en) * 2013-03-28 2014-10-06 Sysmex Corp Reagent for activated partial thromboplastin time measurement and usage thereof
JP2015031586A (en) * 2013-08-02 2015-02-16 株式会社日立ハイテクノロジーズ Analyzer and liquid suction device

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2009008552A (en) * 2007-06-28 2009-01-15 Hitachi High-Technologies Corp Automatic analysis apparatus and dispensing method for automatic analysis apparatus
CN102066947A (en) * 2008-06-18 2011-05-18 积水医疗株式会社 Method for determining factor causing the prolongation of blood coagulation time
CN103688177A (en) * 2011-06-17 2014-03-26 学校法人东日本学园 Method of measuring blood coagulation time to detect lupus anticoagulants
CN104350386A (en) * 2012-06-11 2015-02-11 株式会社日立高新技术 Automatic analysis apparatus

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KAZUHIKO KAGAWA: "Blood coagulation compensation test", 《KENSA TO GIJUTSU》 *

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