CN107430130B - 方法 - Google Patents

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Publication number
CN107430130B
CN107430130B CN201580063963.9A CN201580063963A CN107430130B CN 107430130 B CN107430130 B CN 107430130B CN 201580063963 A CN201580063963 A CN 201580063963A CN 107430130 B CN107430130 B CN 107430130B
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China
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protein
library
binding
target
binding agent
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CN201580063963.9A
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English (en)
Chinese (zh)
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CN107430130A (zh
Inventor
乔鲍·耶奈伊
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Aktone Co ltd
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GENEVILLAGE KFT
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201580063963.9A 2014-11-24 2015-11-24 方法 Active CN107430130B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1420852.4 2014-11-24
GBGB1420852.4A GB201420852D0 (en) 2014-11-24 2014-11-24 Method
PCT/GB2015/053573 WO2016083793A1 (en) 2014-11-24 2015-11-24 Method

Publications (2)

Publication Number Publication Date
CN107430130A CN107430130A (zh) 2017-12-01
CN107430130B true CN107430130B (zh) 2020-07-24

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CN201580063963.9A Active CN107430130B (zh) 2014-11-24 2015-11-24 方法

Country Status (10)

Country Link
US (3) US10605814B2 (https=)
EP (1) EP3224360B1 (https=)
JP (1) JP6871168B2 (https=)
KR (1) KR102422494B1 (https=)
CN (1) CN107430130B (https=)
CA (1) CA2968488C (https=)
ES (1) ES2763563T3 (https=)
GB (1) GB201420852D0 (https=)
SG (2) SG11201703689SA (https=)
WO (1) WO2016083793A1 (https=)

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* Cited by examiner, † Cited by third party
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WO2017214211A1 (en) * 2016-06-09 2017-12-14 Igc Bio, Inc. Methods for identifying a high affinity antibody
CA3051714A1 (en) * 2017-01-31 2018-08-09 The Governors Of The University Of Alberta Display of molecules on silently genetically encoded nanoscale carriers for determining synergistic molecular interactions
US20200355685A1 (en) * 2017-11-06 2020-11-12 Tsinghua University Reagent set for detecting interactions between biomolecules and their regulatory factors, and applications
SG11202100721SA (en) 2018-07-23 2021-02-25 Univ Alberta Genetically-encoded bicyclic peptide libraries
WO2020260277A1 (en) 2019-06-25 2020-12-30 Albert-Ludwig-Universität Freiburg Method and kit for measuring of analytes in bi-component systems and uses thereof
EP4069731A4 (en) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
EP4330678A1 (en) * 2021-04-26 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) High complexity microcompartment-based interaction screening
EP4242656A1 (en) * 2022-03-10 2023-09-13 Actome GmbH Quantitative detection method and corresponding device
CN119698486A (zh) 2022-06-29 2025-03-25 阿克托姆有限责任公司 检测单细胞中的生物分子
GB202218453D0 (en) 2022-12-08 2023-01-25 Actome Gmbh Assay system

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GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
ES2156149T3 (es) 1992-12-04 2001-06-16 Medical Res Council Proteinas de union multivalente y multiespecificas, su fabricacion y su uso.
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
GB0422431D0 (en) * 2004-10-08 2004-11-10 Affitech As Method
ES2448426T3 (es) * 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
GB0903316D0 (en) * 2009-02-26 2009-04-08 Affitech As Method
CN102459591B (zh) 2009-05-20 2015-05-13 诺维莫尼公司 合成性的多肽文库以及用于建立具有天然多样性的多肽变体的方法
CN102549427A (zh) * 2009-07-07 2012-07-04 新加坡科技研究局 鉴定结合配偶体对的方法
WO2012041633A1 (en) * 2010-09-27 2012-04-05 Vipergen A method for making an enriched library
US9209965B2 (en) 2014-01-14 2015-12-08 Microsemi Semiconductor Ulc Network interface with clock recovery module on line card

Non-Patent Citations (9)

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Title
A Novel Helper Phage Enabling Construction of Genome-Scale ORF-Enriched Phage Display Libraries;Amita Gupta et al.;《PLOS》;20130927;第8卷(第9期);全文 *
Autoantigen discovery with a synthetic human peptidome;H Benjamin Larman et al.;《Nature Biotechnology》;20110522;第29卷(第6期);全文 *
By-passing in vitro screening-next generation sequencing technologies applied to antibody display and in silico candidate selection;U. Ravn et al.;《Nucleic Acids Research》;20100915;第38卷(第21期);全文 *
Deep sequencing of phage display libraries to support antibody discovery;Ulla Ravn et al.;《Methods》;20130314;第60卷(第1期);全文 *
From deep sequencing to actual clones;Sara D’Angelo et al.;《Protein Engineering, Design and Selection》;20140901;第27卷(第10期);全文 *
Interaction Analysis through Proteomic Phage Display;Gustav N. Sundell and Ylva Ivarsson;《BioMed Research International》;20140911;第11卷(第5期);全文 *
Large-scale interaction profiling of PDZ domains through proteomic peptide-phage display using human and viral phage peptidomes;Ylva Ivarsson et al.;《PNAS》;20140203;第111卷(第7期);全文 *
ORF phage display to identify cellular proteins with different functions;Wei Li;《Methods》;20120901;第58卷(第1期);全文 *
The antibody mining toolbox;Sara D’Angelo et al.;《MABS》;20131107;第6卷(第1期);全文 *

Also Published As

Publication number Publication date
EP3224360A1 (en) 2017-10-04
JP2017536846A (ja) 2017-12-14
KR20170086627A (ko) 2017-07-26
SG10202008300RA (en) 2020-10-29
US20210003585A1 (en) 2021-01-07
US20230175171A1 (en) 2023-06-08
EP3224360B1 (en) 2019-10-09
CA2968488C (en) 2024-09-10
WO2016083793A1 (en) 2016-06-02
US10605814B2 (en) 2020-03-31
CN107430130A (zh) 2017-12-01
GB201420852D0 (en) 2015-01-07
SG11201703689SA (en) 2017-06-29
KR102422494B1 (ko) 2022-07-19
JP6871168B2 (ja) 2021-05-12
ES2763563T3 (es) 2020-05-29
US20170269098A1 (en) 2017-09-21
CA2968488A1 (en) 2016-06-02
US11421347B2 (en) 2022-08-23

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Patentee before: GENEVILLAGE KFT

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