CA2968488C - METHOD FOR DETECTING CONNECTION INTERSECTIONS - Google Patents

METHOD FOR DETECTING CONNECTION INTERSECTIONS

Info

Publication number
CA2968488C
CA2968488C CA2968488A CA2968488A CA2968488C CA 2968488 C CA2968488 C CA 2968488C CA 2968488 A CA2968488 A CA 2968488A CA 2968488 A CA2968488 A CA 2968488A CA 2968488 C CA2968488 C CA 2968488C
Authority
CA
Canada
Prior art keywords
protein
binding agent
library
target
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2968488A
Other languages
English (en)
French (fr)
Other versions
CA2968488A1 (en
Inventor
Csaba Jeney
Original Assignee
Actome GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Actome GmbH filed Critical Actome GmbH
Publication of CA2968488A1 publication Critical patent/CA2968488A1/en
Application granted granted Critical
Publication of CA2968488C publication Critical patent/CA2968488C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B30/00—Methods of screening libraries
    • C40B30/04—Methods of screening libraries by measuring the ability to specifically bind a target molecule, e.g. antibody-antigen binding, receptor-ligand binding
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1037—Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00—Labels used in chemical analysis of biological material
    • G01N2458/10—Oligonucleotides as tagging agents for labelling antibodies
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2500/00—Screening for compounds of potential therapeutic value
    • G01N2500/04—Screening involving studying the effect of compounds C directly on molecule A (e.g. C are potential ligands for a receptor A, or potential substrates for an enzyme A)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2968488A 2014-11-24 2015-11-24 METHOD FOR DETECTING CONNECTION INTERSECTIONS Active CA2968488C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1420852.4 2014-11-24
GBGB1420852.4A GB201420852D0 (en) 2014-11-24 2014-11-24 Method
PCT/GB2015/053573 WO2016083793A1 (en) 2014-11-24 2015-11-24 Method

Publications (2)

Publication Number Publication Date
CA2968488A1 CA2968488A1 (en) 2016-06-02
CA2968488C true CA2968488C (en) 2024-09-10

Family

ID=52292436

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2968488A Active CA2968488C (en) 2014-11-24 2015-11-24 METHOD FOR DETECTING CONNECTION INTERSECTIONS

Country Status (10)

Country Link
US (3) US10605814B2 (https=)
EP (1) EP3224360B1 (https=)
JP (1) JP6871168B2 (https=)
KR (1) KR102422494B1 (https=)
CN (1) CN107430130B (https=)
CA (1) CA2968488C (https=)
ES (1) ES2763563T3 (https=)
GB (1) GB201420852D0 (https=)
SG (2) SG11201703689SA (https=)
WO (1) WO2016083793A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2017214211A1 (en) * 2016-06-09 2017-12-14 Igc Bio, Inc. Methods for identifying a high affinity antibody
CA3051714A1 (en) * 2017-01-31 2018-08-09 The Governors Of The University Of Alberta Display of molecules on silently genetically encoded nanoscale carriers for determining synergistic molecular interactions
US20200355685A1 (en) * 2017-11-06 2020-11-12 Tsinghua University Reagent set for detecting interactions between biomolecules and their regulatory factors, and applications
SG11202100721SA (en) 2018-07-23 2021-02-25 Univ Alberta Genetically-encoded bicyclic peptide libraries
WO2020260277A1 (en) 2019-06-25 2020-12-30 Albert-Ludwig-Universität Freiburg Method and kit for measuring of analytes in bi-component systems and uses thereof
EP4069731A4 (en) 2019-12-03 2024-05-29 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (nulisa)
EP4330678A1 (en) * 2021-04-26 2024-03-06 Ecole Polytechnique Federale De Lausanne (Epfl) High complexity microcompartment-based interaction screening
EP4242656A1 (en) * 2022-03-10 2023-09-13 Actome GmbH Quantitative detection method and corresponding device
CN119698486A (zh) 2022-06-29 2025-03-25 阿克托姆有限责任公司 检测单细胞中的生物分子
GB202218453D0 (en) 2022-12-08 2023-01-25 Actome Gmbh Assay system

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8308235D0 (en) 1983-03-25 1983-05-05 Celltech Ltd Polypeptides
US4816567A (en) 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
ES2156149T3 (es) 1992-12-04 2001-06-16 Medical Res Council Proteinas de union multivalente y multiespecificas, su fabricacion y su uso.
US7306904B2 (en) 2000-02-18 2007-12-11 Olink Ab Methods and kits for proximity probing
GB0422431D0 (en) * 2004-10-08 2004-11-10 Affitech As Method
ES2448426T3 (es) * 2007-10-12 2014-03-13 Pronota Nv Uso de aptámeros en proteómica
GB0903316D0 (en) * 2009-02-26 2009-04-08 Affitech As Method
CN102459591B (zh) 2009-05-20 2015-05-13 诺维莫尼公司 合成性的多肽文库以及用于建立具有天然多样性的多肽变体的方法
CN102549427A (zh) * 2009-07-07 2012-07-04 新加坡科技研究局 鉴定结合配偶体对的方法
WO2012041633A1 (en) * 2010-09-27 2012-04-05 Vipergen A method for making an enriched library
US9209965B2 (en) 2014-01-14 2015-12-08 Microsemi Semiconductor Ulc Network interface with clock recovery module on line card

Also Published As

Publication number Publication date
EP3224360A1 (en) 2017-10-04
JP2017536846A (ja) 2017-12-14
KR20170086627A (ko) 2017-07-26
SG10202008300RA (en) 2020-10-29
US20210003585A1 (en) 2021-01-07
US20230175171A1 (en) 2023-06-08
EP3224360B1 (en) 2019-10-09
WO2016083793A1 (en) 2016-06-02
US10605814B2 (en) 2020-03-31
CN107430130A (zh) 2017-12-01
GB201420852D0 (en) 2015-01-07
SG11201703689SA (en) 2017-06-29
KR102422494B1 (ko) 2022-07-19
JP6871168B2 (ja) 2021-05-12
ES2763563T3 (es) 2020-05-29
US20170269098A1 (en) 2017-09-21
CA2968488A1 (en) 2016-06-02
US11421347B2 (en) 2022-08-23
CN107430130B (zh) 2020-07-24

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