CN107410784A - A kind of passion fruit diet fiber drink and preparation method thereof - Google Patents

A kind of passion fruit diet fiber drink and preparation method thereof Download PDF

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Publication number
CN107410784A
CN107410784A CN201710600222.0A CN201710600222A CN107410784A CN 107410784 A CN107410784 A CN 107410784A CN 201710600222 A CN201710600222 A CN 201710600222A CN 107410784 A CN107410784 A CN 107410784A
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passion fruit
fruit
shell
diet fiber
liquid
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CN201710600222.0A
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Inventor
辛明
李昌宝
孙健
周主贵
李杰民
唐雅园
零东宁
李丽
刘国明
何雪梅
郑凤锦
盛金凤
李志春
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Institute of Agro Products Processing Science and Technology of Guangxi Academy of Agricultural Sciences
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Institute of Agro Products Processing Science and Technology of Guangxi Academy of Agricultural Sciences
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Priority to CN201710600222.0A priority Critical patent/CN107410784A/en
Publication of CN107410784A publication Critical patent/CN107410784A/en
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/42Preservation of non-alcoholic beverages
    • A23L2/46Preservation of non-alcoholic beverages by heating
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/72Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration
    • A23L2/74Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by filtration using membranes, e.g. osmosis, ultrafiltration
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/80Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/70Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
    • A23L2/84Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105Plant extracts, their artificial duplicates or their derivatives
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/20Reducing nutritive value; Dietetic products with reduced nutritive value
    • A23L33/21Addition of substantially indigestible substances, e.g. dietary fibres
    • A23L33/22Comminuted fibrous parts of plants, e.g. bagasse or pulp
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The present invention provides a kind of passion fruit diet fiber drink and preparation method thereof, belong to food processing field, raw material is used as using the full fruit of passion fruit, by shell segment, acidic protein ferment treatment pulp, ethanol leaching, which is filtered, handles fruit seed, and the passion fruit diet fiber drink that follow-up fermentation obtains with the handling process that homogenizer is combined, it more can completely keep its nutritional ingredient, the passion fruit diet fiber drink instant of gained, mouthfeel is excellent, soluble dietary fibre content therein is high, with abundant nutritive value and good health-care effect, suitable various people eat.

Description

A kind of passion fruit diet fiber drink and preparation method thereof
【Technical field】
The present invention relates to food processing field, especially a kind of passion fruit diet fiber drink and preparation method thereof.
【Technical background】
Passion fruit, also known as passion fruit, it is the herbaceous species plant of Passifloraceae Passiflora, flower garden ornamental plant can be made, 17 kinds of amino acid, multivitamin and carotenoid rich in needed by human body and various trace elements, passion fruit in passion fruit In soluble solid content be 15-16%, total acid content 3.8-4%, the fragrance of passion fruit can releive anxiety and tense, depression All senses of discomfort such as joyless, nervous caused headache, stomachache, frequent micturition, nervous, muscle cramp, twitch, pain.Often feeding Human body is highly profitable with passion fruit and its processed goods, can strengthen body immunity, the superfluorescent fiber in passion fruit can be deep enough The most trickle part of stomach, it is thoroughly discharged by harmful substance in its active gene absorber, and can improved in enteron aisle Flora is formed, and is played the screen-wall effect for protecting stomach not absorb harmful substance, is purified body, avoid harmful substance from sinking in vivo Product, and then improve skin, beautify the effect of appearance, in addition additionally aid digestion, energy resolving sputum promoting blood circulation, sedation-analgesia, Depressurize lipid-loweringing etc..
In summary, passion fruit has higher economic use value, but also exists when processing granadilla food all More problems, such as dietary fiber in passion fruit are difficult to extract, and dietary fiber content is low in the beverage made of the full fruit of passion fruit, Wherein soluble dietary fiber accounting example is low, and nutritional ingredient is low, it is impossible to meets the needs of current consumer groups.
【The content of the invention】
In view of posed problems above, can the invention provides a kind of passion fruit diet fiber drink and preparation method thereof More completely to keep its nutritional ingredient, the passion fruit diet fiber drink instant of gained, mouthfeel is excellent, it is therein can Soluble dietary fiber content is high, and there are abundant nutritive value and good health-care effect, suitable various people to eat.
Technical scheme is as follows:
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2-3 times of quality, in the shell that step (1) is isolated soaks 30- 40min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 15-20min under the conditions of being 40-42 DEG C in temperature, then handled with 300-350MPa instantaneous pressure 10-20s, obtain solidliquid mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed thereto The ethanol solution of 3-4 times of volume, after soaking 1-1.5h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues to impregnate 24- After 25h, whole filtrates are filtered out as continuous liquid of filtering, and will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, are by gained liquid For mixed extract;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) mixed extract is put into superhigh-voltage homogenizing machine, is added active liquid by 12-15g/100g dosage, is in pressure 5-10min is handled under conditions of 160-180MPa;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, then gained filter liquor is used into superhigh temperature wink When sterilize, it is filling, the passion fruit diet fiber drink is formed after natural cooling.
Further, in step (5), described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into In formula slant medium, 6-8h is cultivated at 23-25 DEG C, the spore of cultured bacterial strain is then put into the cone equipped with nutrient solution In shape bottle, 30-35min is vibrated under conditions of temperature is 25-28 DEG C, hunting speed is 120-140r/min, is then diluted to Concentration is 106-9Cfu/mL bacteria suspension, then bacteria suspension is placed in the vibration case at uviol lamp 25-35cm, in The lower reaction 20-25s of 150-180r/min hunting speed, obtains active liquid.
Further, described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, Water is according to 1:0.2-0.3:0.1-0.15:0.2-0.3:2-3:1000 mass ratio is mixed.
Further, in step (3), the usage amount of the acid protease is passion fruit fruit juice pulp mixture 0.03-0.04wt%.
Further, in step (4), the volumetric concentration of the ethanol is 65-70%.
Further, in step (6), the material obtained by step (5) is after Ultra filtration membrane, in addition to decolorization process, Wherein, decolorising agent used in decolorization is cocoanut active charcoal.
Present invention additionally comprises using the passion fruit diet fiber drink obtained by above preparation method.
The invention provides a kind of passion fruit diet fiber drink and preparation method thereof, compared with prior art, have with Lower beneficial effect:
1. the raw material used in the passion fruit diet fiber drink of the present invention includes the full fruit of passion fruit, preparation method of the invention The dietary fiber in passion fruit shell, pulp and fruit seed can be effectively extracted, and then improves the content of dietary fiber in beverage.This Invention first separates the shell, pulp and fruit seed of passion fruit, carries out preliminary pretreatment for the characteristic of various pieces, so Carry out enzymolysis processing using homemade active liquid afterwards, finally by Ultra filtration membrane, decolouring, sterilizing, filling obtain the present invention's Beverage.Wherein, passion fruit fruit seed with pulp separation process is separated by the mode of vacuum filtration, meanwhile, vacuum filtration The flesh cell of passion fruit can be made to bear huge pressure differential in a short time, force cell rupture, intracellular organized enzyme, nothing These absorbable nutriments of machine salt, polysaccharide and lipid can be released, and be dissolved in the solution, in order to follow-up Carrying out extraction has more effective beneficiating ingredients and dietary fiber, and is easier to be absorbed by the body, and these nutritional ingredients In pectin, flavone compound and organized enzyme can promote the digestion and absorption of human body, further enhance consumer groups The digestive function of stomach;Secondly, preliminary pretreatment, the preprocess method of passion fruit pulp are carried out to the various pieces of passion fruit It is that will wherein add acidic protein ferment treatment, the starch in passion fruit pulp can be improved, flavour of food products, improvement can be improved Quality;The preprocess method of passion fruit fruit seed is after passing it through pulverization process, then adds ethanol solution thereto and carry out leaching and filter, The volatile oil, flavonoids, triterpenes isoreactivity thing composition that alcohol is soluble in fruit seed are extracted can to greatest extent, so as to improve The nutritive value of product;Then, enzymolysis processing, wherein enzymolysis processing are carried out to the material after above-mentioned processing and passion fruit shell Carried out by spraying homemade active liquid in superhigh-voltage homogenizing machine, make enzymolysis process compound in turbulent flow, hole, shearing etc. Carried out in the presence of power, products obtained therefrom more homogeneous, emulsification, and then improve the extracted amount of passion fruit dietary fiber can be made;Finally, Gained material is passed through into Ultra filtration membrane, the soluble dietary fiber in passion fruit, soluble dietary fiber can be efficiently separated out With different physiological roles, including prevention of cardiovascular disease, diabetes and antitumor action.Each technology of the present invention Means all cooperate, mutually promoted, and camp step by step, be all linked with one another, and caused total technique effect is much high In technological means caused by single technological means simple plus and.
2. the technique of the active liquid prepared by the present invention can efficiently prepare cellulase, and then be cellulase degradation mistake Cheng Suoxu cellulose and hemicellulose lays the first stone.The present invention is obtained using trichoderma viride as strain using Homemade method Nutrient solution is cultivated, wherein, the nutrient solution is according to spy by ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water Fixed ratio is made, the requirement when disclosure satisfy that trichoderma viride mutagenesis in the presence of nutrient solution to osmotic pressure, to promote Enter trichoderma viride mutagenesis provide energy, the present invention employed in technological means cooperate, mutually promote, from different aspect Promote the preparation of cellulase with angle, the efficiency that raising prepares cellulase is substantially improved.
In a word, passion fruit diet fiber drink provided by the invention and preparation method thereof, more can completely keep it Nutritional ingredient, the passion fruit diet fiber drink instant of gained, mouthfeel is excellent, soluble dietary fibre content therein Height, there are abundant nutritive value and good health-care effect, suitable various people to eat.
【Embodiment】
The following examples can help those skilled in the art that the present invention is more completely understood, but cannot be with any Mode limits the present invention.
Embodiment 1
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2 times of quality, in the shell that step (1) is isolated soaks 30min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 15min under the conditions of being 40 DEG C in temperature, then handle 10s with 300MPa instantaneous pressure, consolidate Liquid mixture;Wherein, the usage amount of the acid protease is the 0.03wt% of passion fruit fruit juice pulp mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed 3 thereto The ethanol solution of times volume, after soaking 1h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues after impregnating 24h, filters out Whole filtrates are continuous liquid of filtering, and will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, gained liquid is as mixed into extraction Thing;Wherein, the volumetric concentration of the ethanol is 65%;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) mixed extract is put into superhigh-voltage homogenizing machine, and active liquid is added by 12g/100g dosage, in pressure be 160MPa Under conditions of handle 5min;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 6h is cultivated at 23 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 25 DEG C, Hunting speed vibrates 30min under conditions of being 120r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 25cm, in the lower reaction 20s of 150r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.2:0.1:0.2:2:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Embodiment 2
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 3 times of quality, in the shell that step (1) is isolated soaks 40min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 20min under the conditions of being 42 DEG C in temperature, then handle 20s with 350MPa instantaneous pressure, consolidate Liquid mixture;Wherein, the usage amount of the acid protease is the 0.04wt% of passion fruit fruit juice pulp mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed 4 thereto The ethanol solution of times volume, after soaking 1.5h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and is continued after impregnating 25h, filtering Go out whole filtrates for continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, gained liquid is as mixed into extraction Thing;Wherein, the volumetric concentration of the ethanol is 70%;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) mixed extract is put into superhigh-voltage homogenizing machine, and active liquid is added by 15g/100g dosage, in pressure be 180MPa Under conditions of handle 10min;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 8h is cultivated at 25 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 28 DEG C, Hunting speed vibrates 35min under conditions of being 140r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 35cm, in the lower reaction 25s of 180r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.3:0.15:0.3:3:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Embodiment 3
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2.5 times of quality, in the shell that step (1) is isolated soaks 35min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 18min under the conditions of being 41 DEG C in temperature, then handle 15s with 320MPa instantaneous pressure, consolidate Liquid mixture;Wherein, the usage amount of the acid protease is the 0.035wt% of passion fruit fruit juice pulp mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed thereto The ethanol solution of 3.5 times of volumes, after soaking 1.2h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues to impregnate 24.5h Afterwards, whole filtrates are filtered out as continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, are by gained liquid Mixed extract;Wherein, the volumetric concentration of the ethanol is 68%;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) mixed extract is put into superhigh-voltage homogenizing machine, and active liquid is added by 13g/100g dosage, in pressure be 175MPa Under conditions of handle 8min;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 7h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 26 DEG C, Hunting speed vibrates 32min under conditions of being 130r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 30cm, in the lower reaction 22s of 160r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.25:0.12:0.25:2.5:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Comparative example 1
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2.5 times of quality, in the shell that step (1) is isolated soaks 35min, acid protease is then added thereto, 18min is handled under the conditions of being 41 DEG C in temperature, then with 320MPa moment HIGH PRESSURE TREATMENT 15s, obtains solidliquid mixture;Wherein, the usage amount of the acid protease is passion fruit fruit juice pulp mixture 0.035wt%;
(4) enzymolysis processing:Passion fruit shell section, step (3) described solidliquid mixture after step (2) is cut are put into super In high pressure homogenizer, active liquid is added by 13g/100g dosage, 8min is handled under conditions of pressure is 175MPa;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 7h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 26 DEG C, Hunting speed vibrates 32min under conditions of being 130r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 30cm, in the lower reaction 22s of 160r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.25:0.12:0.25:2.5:1000 mass ratio is mixed;
(5) finished product:Material obtained by step (4) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Comparative example 2
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2.5 times of quality, in the shell that step (1) is isolated soaks 35min, passion fruit seed therein is taken out by the way of vacuum filtration, it is standby to retain much filtrate;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed thereto The ethanol solution of 3.5 times of volumes, after soaking 1.2h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues to impregnate 24.5h Afterwards, whole filtrates are filtered out as continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, are by gained liquid Mixed extract;Wherein, the volumetric concentration of the ethanol is 68%;
(5) enzymolysis processing:Passion fruit shell section, step (3) much filtrate after step (2) is cut, step (4) institute State mixed extract to be put into superhigh-voltage homogenizing machine, active liquid is added by 13g/100g dosage, in the bar that pressure is 175MPa 8min is handled under part;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 7h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 26 DEG C, Hunting speed vibrates 32min under conditions of being 130r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 30cm, in the lower reaction 22s of 160r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.25:0.12:0.25:2.5:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Comparative example 3
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2.5 times of quality, in the shell that step (1) is isolated soaks 35min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 18min under the conditions of being 41 DEG C in temperature, then handle 15s with 320MPa instantaneous pressure, consolidate Liquid mixture;Wherein, the usage amount of the acid protease is the 0.035wt% of passion fruit fruit juice pulp mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed thereto The ethanol solution of 3.5 times of volumes, after soaking 1.2h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues to impregnate 24.5h Afterwards, whole filtrates are filtered out as continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, are by gained liquid Mixed extract;Wherein, the volumetric concentration of the ethanol is 68%;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) active liquid is added by 13g/100g dosage in the mixed extract, 8min is handled under conditions of pressure is 175MPa;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 7h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 26 DEG C, Hunting speed vibrates 32min under conditions of being 130r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 30cm, in the lower reaction 22s of 160r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1: 0.25:0.12:0.25:2.5:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Comparative example 4
A kind of preparation method of passion fruit diet fiber drink, comprises the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell Separated with material in shell standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2.5 times of quality, in the shell that step (1) is isolated soaks 35min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 18min under the conditions of being 41 DEG C in temperature, then handle 15s with 320MPa instantaneous pressure, consolidate Liquid mixture;Wherein, the usage amount of the acid protease is the 0.035wt% of passion fruit fruit juice pulp mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds fruit seed thereto The ethanol solution of 3.5 times of volumes, after soaking 1.2h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and continues to impregnate 24.5h Afterwards, whole filtrates are filtered out as continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, are by gained liquid Mixed extract;Wherein, the volumetric concentration of the ethanol is 68%;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) mixed extract is put into superhigh-voltage homogenizing machine, and active liquid is added by 13g/100g dosage, in pressure be 175MPa Under conditions of handle 8min;
Wherein described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, 7h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into the conical flask equipped with nutrient solution, temperature be 26 DEG C, Hunting speed vibrates 32min under conditions of being 130r/min, is then diluted to concentration as 106-9Cfu/mL bacteria suspension, then will Bacteria suspension is placed in the vibration case at uviol lamp 30cm, in the lower reaction 22s of 160r/min hunting speed, is lived Property liquid;
Described nutrient solution includes following raw material:Ammonium nitrate, water are according to 1:1000 mass ratio is mixed;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, decolourized, then gained filter liquor is used into superelevation Warm instantaneous sterilizing, it is filling, the passion fruit diet fiber drink is formed after natural cooling;Wherein, decolorising agent used in decolorization For cocoanut active charcoal.
Experimental example
1. sensory evaluation is tested:Using system point system, group is evaluated for the composition of personnel of Food Science by 50 specialties, The passion fruit diet fiber drink prepared by embodiment 1-3 and comparative example 1-4 preparation method, carries out subjective appreciation.Evaluation refers to Color and luster, flavour and smell, tissue morphology are designated as, it is 30 points to take the total score of its each individual event average value as appraisal result, total score, is commented Minute mark standard is shown in Table 1, and appraisal result is shown in Table 2.
The sensory evaluation scores standard of the passion fruit diet fiber drink of table 1
The sensory evaluation scores result of the passion fruit diet fiber drink of table 2
The result of table 2 shows:Total score is higher, illustrates that the quality of the passion fruit diet fiber drink of preparation is better.Comparative example 1 Compared with Example 3, the pulp of passion fruit is not pre-processed respectively with fruit seed, the taste of products obtained therefrom is slightly puckery and mouthfeel It is not fine and smooth enough;Compared with Example 3, passion fruit pulp is inadequate without acidic protein ferment treatment, products obtained therefrom color for comparative example 2 Limpid, taste is not salubrious enough;Compared with Example 3, enzymolysis process is not put in superhigh-voltage homogenizing machine and carried out comparative example 3, gained Products taste is not fine and smooth enough, not enough clearly;Compared with Example 3, nutrient solution is made up comparative example 4 of ammonium nitrate, water, causes it Product colour skewness, it is not fine and smooth enough, illustrate that nutrition liquid energy of the present invention greatly improves the cellulase of product Content, so as to strengthen the hydrolysis result of enzymolysis process, so as to improve quality and mouthfeel.
2. drink effect test
(1)
Experiment is divided into 7 groups, and the volunteer of every group of each selection 20 35-40 year, each group drinks embodiment 1-3 and contrast daily The beverage that example 1-4 makes is drunk 2 times, the moisture of volume glabella skin is determined after drinking 6 months, during this period, volunteer must not take With the product of other improvement moisture of skin, concrete outcome is shown in Table 3.
The health-care effect of the passion fruit diet fiber drink of table 3
Embodiment 1 Embodiment 2 Embodiment 3 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4
Moisture of skin (%) 41.2 41.8 42.6 35.8 36.9 36.5 37.3
The result of table 3 shows:Embodiment 1-3 volunteer's moisture of skin is higher, illustrates that often drink the present invention hundred is fragrant Fruit diet fiber drink, moisture of skin can be improved, the water and oil balance of balanced skin, improves skin, so as to reach beauty The purpose of beauty treatment.
(2)
Cough-relieving is tested:Experiment mice is divided into 7 groups, every group of each raising 10.Embodiment 1-3 and contrast are fed daily by each group Beverage (2ml/100g body weight) prepared by example 1-4, common 20d.
During experiment, the experiment mice is to carry out drawing cough using the concentrated ammonia liquor of excusing from death atomizer spray 25%, establishes mouse cough Cough model, the cough number in cough 2min occurs in record;Beverage of the present invention is fed daily, and last, which feeds rear 1h, makes mouse again Receive above-mentioned spraying, the cough number in cough 2min occurs in record;All it the results are shown in Table 6.
The health-care effect of the passion fruit diet fiber drink of table 4
The result of table 4 shows:1-3 of embodiment of the present invention beverage can substantially reduce the cough number of mouse, illustrate this The product of invention can effectively reach the effect of moistening the lung and relieve the cough.
(3)
Emptying test:Experimental animal adapts to raising 1 week, is divided into 7 groups, and every group is respectively raised 10 experimental animals.Each group Passion fruit diet fiber drink (2ml/100g body weight) prepared by embodiment 1-3 and comparative example 1-4, common 30d are fed daily.
Every rat establishes rat gastric emptying delay model, fed daily by the way that Dopamine hydrochloride is injected intraperitoneally during experiment Passion fruit diet fiber drink, last feeds rear 1h and takes off neck execution, using stomach full weight and the difference of stomach net weight as residue weight in stomach, It is Stomach residue rate that residue, which accounts for and fills the semi-solid percentage for pasting weight, in calculating stomach;All it the results are shown in Table 5.
The health-care effect of the passion fruit diet fiber drink of table 5
Embodiment 1 Embodiment 2 Embodiment 3 Comparative example 1 Comparative example 2 Comparative example 3 Comparative example 4
Residual rate (%) 61.6 62.9 60.2 85.2 86.4 89.5 79.9
The result of table 5 shows:1-3 of embodiment of the present invention passion fruit diet fiber drink can make the residual of food in Mouse Stomach The effect of allowance is reduced, and food digestion can be effectively facilitated by illustrating the product of the present invention.
Although above the present invention is described in detail with a general description of the specific embodiments, On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.

Claims (7)

1. a kind of preparation method of passion fruit diet fiber drink, it is characterised in that comprise the following steps:
(1) raw material:Passion fruit ripe, that shell is full is chosen as raw material, after being cleaned up, by passion fruit shell and fruit The separation of object in the shell matter is standby;
(2) shell pre-processes:The section that the passion fruit shell that step (1) is isolated is cut into 1-2cm is standby;
(3) pulp pre-processes:Material, which is put into the water of its 2-3 times of quality, in the shell that step (1) is isolated soaks 30- 40min, passion fruit seed therein is taken out by the way of vacuum filtration, will added in the passion fruit fruit juice pulp mixture filtered out Enter acid protease, handle 15-20min under the conditions of being 40-42 DEG C in temperature, then handled with 300-350MPa instantaneous pressure 10-20s, obtain solidliquid mixture;
(4) fruit seed pre-processes:The fruit seed that step (3) separates is put into pulverizer again and crushed, then adds 3-4 times of fruit seed thereto The ethanol solution of volume, after soaking 1-1.5h, the filtrate that 10% volume is taken out in filtering is liquid of just filtering, and is continued after impregnating 24-25h, Whole filtrates are filtered out as continuous liquid of filtering, will just filter liquid and continuous liquid of filtering merge, and after recovery removes ethanol, gained liquid is as mixed Extract;
(5) enzymolysis processing:Passion fruit shell section, step (3) solidliquid mixture after step (2) is cut, step (4) institute State mixed extract to be put into superhigh-voltage homogenizing machine, active liquid added by 12-15g/100g dosage, in pressure be 160- 5-10min is handled under conditions of 180MPa;
(6) finished product:Material obtained by step (5) is passed through into Ultra filtration membrane, then gained filter liquor is instantaneously gone out using superhigh temperature Bacterium, it is filling, the passion fruit diet fiber drink is formed after natural cooling.
A kind of 2. preparation method of passion fruit diet fiber drink according to claim 1, it is characterised in that step (5) In, described active liquid is prepared by following methods:Trichoderma viride strain is forwarded to and looked into formula slant medium, at 23-25 DEG C Lower culture 6-8h, is then put into the spore of cultured bacterial strain in the conical flask equipped with nutrient solution, temperature be 25-28 DEG C, Hunting speed vibrates 30-35min under conditions of being 120-140r/min, is then diluted to concentration as 106-9Cfu/mL bacterium is hanged Liquid, then bacteria suspension is placed in the vibration case at uviol lamp 25-35cm, in 150-180r/min hunting speed Lower reaction 20-25s, obtains active liquid.
A kind of 3. preparation method of passion fruit diet fiber drink according to claim 2, it is characterised in that described battalion Nutrient solution includes following raw material:Ammonium nitrate, magnesium chloride, ferric sulfate, sodium chloride, glucose, water are according to 1:0.2-0.3:0.1-0.15: 0.2-0.3:2-3:1000 mass ratio is mixed.
4. a kind of preparation method of passion fruit diet fiber drink according to claim 1, it is characterised in that in step (3) In, the usage amount of the acid protease is the 0.03-0.04wt% of passion fruit fruit juice pulp mixture.
5. a kind of preparation method of passion fruit diet fiber drink according to claim 1, it is characterised in that in step (4) In, the volumetric concentration of the ethanol is 65-70%.
A kind of 6. passion fruit diet fiber drink according to claim 1, it is characterised in that in step (6), step (5) The material of gained is after Ultra filtration membrane, including decolorization process;Wherein, decolorising agent used in decolorization is cocoanut active charcoal.
7. the passion fruit diet fiber drink that the preparation method described in claim any one of 1-6 obtains.
CN201710600222.0A 2017-07-21 2017-07-21 A kind of passion fruit diet fiber drink and preparation method thereof Pending CN107410784A (en)

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