CN107347984A - A kind of passion fruit antistaling agent and preparation method thereof and application - Google Patents
A kind of passion fruit antistaling agent and preparation method thereof and application Download PDFInfo
- Publication number
- CN107347984A CN107347984A CN201710600590.5A CN201710600590A CN107347984A CN 107347984 A CN107347984 A CN 107347984A CN 201710600590 A CN201710600590 A CN 201710600590A CN 107347984 A CN107347984 A CN 107347984A
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- Prior art keywords
- passion fruit
- parts
- antistaling agent
- fruit
- pericarp
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- 235000000370 Passiflora edulis Nutrition 0.000 title claims abstract description 125
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 79
- 239000000463 material Substances 0.000 claims abstract description 69
- 238000000034 method Methods 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 29
- 235000004347 Perilla Nutrition 0.000 claims abstract description 22
- 238000012545 processing Methods 0.000 claims abstract description 21
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- 235000002017 Zea mays subsp mays Nutrition 0.000 claims abstract description 20
- 235000005822 corn Nutrition 0.000 claims abstract description 20
- 235000009051 Ambrosia paniculata var. peruviana Nutrition 0.000 claims abstract description 19
- 241000205585 Aquilegia canadensis Species 0.000 claims abstract description 19
- 235000003097 Artemisia absinthium Nutrition 0.000 claims abstract description 19
- 240000001851 Artemisia dracunculus Species 0.000 claims abstract description 19
- 235000017731 Artemisia dracunculus ssp. dracunculus Nutrition 0.000 claims abstract description 19
- 235000003261 Artemisia vulgaris Nutrition 0.000 claims abstract description 19
- 235000009419 Fagopyrum esculentum Nutrition 0.000 claims abstract description 19
- 235000013717 Houttuynia Nutrition 0.000 claims abstract description 19
- 239000001138 artemisia absinthium Substances 0.000 claims abstract description 19
- 238000002156 mixing Methods 0.000 claims abstract description 19
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims abstract description 19
- 238000000605 extraction Methods 0.000 claims abstract description 12
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 46
- 239000000203 mixture Substances 0.000 claims description 32
- 239000002994 raw material Substances 0.000 claims description 23
- 235000015097 nutrients Nutrition 0.000 claims description 19
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 18
- 229910021538 borax Inorganic materials 0.000 claims description 17
- 238000002425 crystallisation Methods 0.000 claims description 17
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- 230000008569 process Effects 0.000 claims description 15
- 238000003860 storage Methods 0.000 claims description 15
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 10
- 238000001035 drying Methods 0.000 claims description 10
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 9
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 9
- 239000000706 filtrate Substances 0.000 claims description 9
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 9
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- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 9
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- 239000000843 powder Substances 0.000 description 6
- 235000002639 sodium chloride Nutrition 0.000 description 6
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- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
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- 239000011734 sodium Substances 0.000 description 2
- 229910052708 sodium Inorganic materials 0.000 description 2
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- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 description 1
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- IAJILQKETJEXLJ-RSJOWCBRSA-N aldehydo-D-galacturonic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-RSJOWCBRSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000036506 anxiety Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
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- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 150000002216 flavonol derivatives Chemical class 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23B—PRESERVING, e.g. BY CANNING, MEAT, FISH, EGGS, FRUIT, VEGETABLES, EDIBLE SEEDS; CHEMICAL RIPENING OF FRUIT OR VEGETABLES; THE PRESERVED, RIPENED, OR CANNED PRODUCTS
- A23B7/00—Preservation or chemical ripening of fruit or vegetables
- A23B7/14—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10
- A23B7/153—Preserving or ripening with chemicals not covered by groups A23B7/08 or A23B7/10 in the form of liquids or solids
- A23B7/154—Organic compounds; Microorganisms; Enzymes
- A23B7/155—Microorganisms; Enzymes; Antibiotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y402/00—Carbon-oxygen lyases (4.2)
- C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
- C12Y402/0201—Pectin lyase (4.2.2.10)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention provides a kind of passion fruit antistaling agent and preparation method thereof and application, belong to food processing field, first by passion fruit shell, grape fruit pericarp, active liquid is added after orange pericarp segment to be handled to obtain material a in superhigh-voltage homogenizing machine, again by perilla leaf, konjaku, corn stigma crushes, material b is extracted using subcritical extraction method after immersion, then by material a, material b and in Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, the material c mixing extracted in oriental wormwood, which is put into high pressure microjet nano-dispersed machine, to be disperseed to obtain antistaling agent, the present invention also provides the application method of the antistaling agent.The passion fruit antistaling agent that the present invention obtains is safe and non-toxic, phenomena such as can preventing passion fruit fruit from producing weightless, shrinkage, can maintain the outward appearance of passion fruit and its quality of pulp, extend the pot-life of passion fruit.
Description
【Technical field】
The present invention relates to food processing field, especially a kind of passion fruit antistaling agent and preparation method thereof and application.
【Technical background】
Passion fruit, also known as passion fruit, it is the herbaceous species plant of Passifloraceae Passiflora, flower garden ornamental plant can be made,
17 kinds of amino acid, multivitamin and carotenoid rich in needed by human body and various trace elements, passion fruit in passion fruit
In soluble solid content be 15-16%, total acid content 3.8-4%, the fragrance of passion fruit can releive anxiety and tense, depression
All senses of discomfort such as joyless, nervous caused headache, stomachache, frequent micturition, nervous, muscle cramp, twitch, pain.Often feeding
Human body is highly profitable with passion fruit and its processed goods, can strengthen body immunity, the superfluorescent fiber in passion fruit can be deep enough
The most trickle part of stomach, it is thoroughly discharged by harmful substance in its active gene absorber, and can improved in enteron aisle
Flora is formed, and is played the screen-wall effect for protecting stomach not absorb harmful substance, is purified body, avoid harmful substance from sinking in vivo
Product, and then improve skin, beautify the effect of appearance, in addition additionally aid digestion, energy resolving sputum promoting blood circulation, sedation-analgesia,
Depressurize lipid-loweringing etc..
In summary, passion fruit has higher economic use value, but fresh passion fruit can only be store under the conditions of 6.5-7 DEG C
Deposit 4-5 weeks, passion fruit belongs to climacteric type fruit, acetate releasing quantity hourly at normal temperatures after the fruit of physiological maturity is adopted
Up to 160-370ml/kg, the easy atrophy of fruit rind is weightless, passion fruit skin can a large amount of dehydrations, and pulp can ferment liquefaction, acceleration
The shrinkage of shell, and pericarp is easily gone mouldy by infection process;And passion fruit is easily cooled to low-temperature sensitive, conventional cryopreservation
Evil.So passion fruit this not storage endurance the characteristics of greatly inhibit the development in its market at home, particularly northern market
Development so that the north almost lose passion fruit sale.
It is one of mainstream technology for being used for fruit fresh-keeping in recent years using multifunctional coating coating fruit surface at present, it is this
The advantage of method is to cross extension storage phase really, and fruit glossiness is high, is pacified however as consumer for these edible fruit waxes
The worry of full hidden danger, it is impossible to meet the needs of current consumer groups.
【The content of the invention】
In view of posed problems above, the invention provides a kind of passion fruit antistaling agent and preparation method thereof and application, institute
Obtained passion fruit antistaling agent is safe and non-toxic, does not add chemical preservative, can also prevent passion fruit fruit from producing weightlessness, shrinkage etc.
Phenomenon, the outward appearance of passion fruit and its quality of pulp can be maintained, extend the pot-life of passion fruit.
Technical scheme is as follows:
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without the passion fruit shell 35-40 part, grape fruit pericarp 10-15 parts, orange pericarp gone mouldy
25-30 parts are used as raw material, are mixed after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 5-8mm section mixing
Compound a, mixture a is put into superhigh-voltage homogenizing machine, active liquid is added by 10-12g/100g dosage, in pressure be 170-
5-10min is handled under conditions of 190MPa, obtains material a;
(2) perilla leaf 8-10 parts, konjaku 15-20 parts, corn stigma 5-8 parts are chosen according to parts by weight, by perilla leaf, konjaku,
It is 2-3mm that corn stigma, which is crushed to granularity, is put in after mixing in the cold water of its 2-3 times of quality, under 10-15KHz sound wave environment
Immersion 40-45min obtains mixture b, mixture b is put into more stainless steel extractors, using subcritical extraction method, Yu Wen
Spend for 125-135 DEG C, pressure be 2-3MPa under conditions of handle 26-30min, collect filter liquor and obtain material b;
(3) Buckwheat Flower 4-6 parts, honeysuckle 2-3 parts, Herba Andrographitis 1-2 parts, cordate houttuynia 2-3 parts, radix scutellariae are chosen according to parts by weight
1-2 parts, folium isatidis 2-3 parts, oriental wormwood 1-2 parts, Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood are passed through
After washing, 60-70 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 20- under conditions of pH is constant
22min, borax is then added, half an hour filtering is incubated at 95-100 DEG C, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, then
25-28 DEG C is cooled to, stands and separates out crystallization, separating obtained crystallization is material c;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 150-200MPa, flow velocity 190-260ml/min.
Further, in step (1), described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to Ma Ling
In potato glucose agar medium, 20-25h is cultivated at 23-25 DEG C, the spore of cultured bacterial strain is then put into conical flask
In, 8-10min is shaken, adjustment concentration is 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in being 26-28 in temperature
DEG C, hunting speed be 160-200r/min under conditions of vibrate 30-35min, obtain active liquid.
Further, described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, chlorine
Change sodium, glucose, water according to 1-2:0.2-0.3:0.03-0.05:0.02-0.03:2-3:1000 mass ratio is mixed.
Further, in step (3), the mass concentration of the borax is 0.35-0.45%.
Present invention additionally comprises using the passion fruit antistaling agent obtained by above preparation method.
The present invention also provides a kind of application process of passion fruit antistaling agent, and described antistaling agent is used for the fresh-keeping storage of passion fruit
Deposit, the antistaling agent is used after the water of 20-40 times of addition thereto is well mixed;Its application method is:By passion fruit
Dry, taken out after being put in antistaling agent middle standing 35-40min at ambient temperature, then low temperature drying after surface clean is clean
To surface without obvious water stain, you can stored.
The invention provides a kind of passion fruit antistaling agent and preparation method thereof and application, compared with prior art, have with
Lower beneficial effect:
1. the raw material used in the passion fruit antistaling agent of the present invention includes passion fruit shell, grape fruit pericarp, orange pericarp, purple
Perilla leaf, konjaku, corn stigma, Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood, pass through the system of the present invention
Effective ingredient in above-mentioned plant can effectively be extracted by making method, first by passion fruit shell, grape fruit pericarp, orange pericarp
Add homemade active liquid be put in superhigh-voltage homogenizing machine carry out enzymolysis processing obtain material a, make enzymolysis process in turbulent flow, shearing
Etc. carrying out in the presence of composite force, enzymolysis product more homogeneous, emulsification can be made, and then improve ferment effect, resulting material a
Under the collective effect of active liquid box superhigh-voltage homogenizing machine, pectin decomposer is more completely converted into, there is fungistatic effect, and
It is harmless to the human body, wherein, raw materials used is the Fruit Peel rich in pectin composition, and these materials are that fruit process is discarded
Thing, the present invention have re-used this part resource, not only solve the handling problems of residual waste, moreover it is possible to greatly improve pectin point
The yield of thing is solved, lowers production cost, and volatile oil is rich in the mixture of passion fruit shell, grape fruit pericarp, orange pericarp
Passion fruit smell can be kept Deng material, Shelf-life;It is put in after again crushing perilla leaf, konjaku, corn stigma under sound wave environment
Immersion, material b is then obtained using subcritical extraction method, under given conditions extraction, subcritical abstraction compares other separation
Method has many advantages, and the active component that its nontoxic, non-thermal technology can retain extract is not damaged and aoxidized, and passes through Asia
Containing materials such as mannosans in the material b that critical method for extraction is extracted to obtain by perilla leaf, konjaku, corn stigma, have and suppress
Various bacteria and oxidation resistant effect, the pericarp of passion fruit and the rotten fresh journey for becoming speed, maintaining passion fruit of pulp can be alleviated
Degree;Secondly, Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood are mixed according to special ratios and is put in pH
Boil under conditions of=7.5-8, be incubated after adding borax, then filtrate is adjusted to stand after pH=6-7 and separates out crystallization as material
C, the collocation of above-mentioned raw materials and component are combined into one group of raw material in the applicant's achievement in research with preferable effect, the group
Raw material can suppress the breeding of microorganism, bacterium to a certain extent, so as to alleviate the spoilage rate of passion fruit nutritional ingredient, make
Contained slow nutritional ingredient is largely extracted conversion in each raw material, and nutriment can be made to pass through the thing that the above method extracts
Matter c is more completely changed, and contained flavonols glycocide has the elasticity of enhancing passion fruit epidermal cell wall and suppresses thin in material c
The effect of bacterium and its oxidation, the coat color of passion fruit can be kept, slow down fruit senescence speed, so as to prevent passion fruit fruit
Real phenomena such as producing weightlessness, shrinkage, the outward appearance of passion fruit is maintained, further improves its fresh-keeping effect;Finally by material a, material
B, material c is put into progress nano-dispersed processing in high pressure microjet nano-dispersed machine, obtains passion fruit antistaling agent and is covered in hundred perfume
The epidermis of fruit can also maintain appropriate permeability, water preventing ability, can increase penetration resistance to various gas molecules, form one kind
Micro- controlled atmosphere environment, increase the carbon dioxide content in fruits and vegetables tissue, oxygen content reduce, it is suppressed that the respiratory metabolism of fruits and vegetables and
Moisture loss, slow down fruits and vegetables tissue and structure aging, fruits and vegetables are carried out so as to effectively fresh-keeping.Each skill of the present invention
Art means all cooperate, mutually promoted, and camp step by step, be all linked with one another, and caused total technique effect is much
Higher than technological means caused by single technological means simple plus and.
2. the technique of the active liquid prepared by the present invention can efficiently prepare pectase, and then be pectinase enzymatic hydrolysis process institute
The galacturonic acid enzyme and galacturonic acid oligosaccharides enzyme needed lays the first stone.The present invention is using aspergillus niger as strain, using self-control side
Method obtains nutrient solution and cultivated, wherein, the nutrient solution is by ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, chlorination
Sodium, glucose, water are made according to specific ratio, when disclosure satisfy that aspergillus niger mutagenesis in the presence of nutrient solution to infiltration
The requirement of pressure, to promote aspergillus niger mutagenesis to provide energy, technological means mutual cooperation employed in the present invention, mutually promote,
Promote the preparation of pectase from different aspect and angle, the efficiency that raising prepares pectase is substantially improved.
3. the present invention also provides the application process of made antistaling agent, available for the fresh-keeping storage to passion fruit, it makes
With method to be simple and convenient, be by passion fruit surface clean it is clean after dry, be put in the antistaling agent after dilution, and in room temperature bar
Taken out after standing 35-40min under part, then low temperature drying to surface is without obvious water stain, you can stored using conventional method,
The shelf life of passion fruit can be extended.
In a word, passion fruit antistaling agent provided by the invention and preparation method thereof and application, resulting passion fruit antistaling agent
It is safe and non-toxic, chemical preservative is not added, phenomena such as can also preventing passion fruit fruit from producing weightless, shrinkage, passion fruit can be maintained
Outward appearance and its pulp quality, extend pot-life of passion fruit.
【Embodiment】
The following examples can help those skilled in the art that the present invention is more completely understood, but cannot be with any
Mode limits the present invention.
Embodiment 1
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 25 parts of 10 parts of 35 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 5mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 10g/100g dosage, is handled under conditions of pressure is 170MPa
5min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 20h is cultivated at 23 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 8min, adjust concentration
For 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 26 DEG C, hunting speed is 160r/min
Lower vibration 30min, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 1:0.2:0.03:0.02:2:1000 mass ratio is mixed;
(2) 8 parts of perilla leaf, 15 parts of konjaku, 5 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 2mm to be broken to granularity, is put in after mixing in the cold water of its 2 times of quality, and 40min is soaked under 10KHz sound wave environment and is mixed
Compound b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 125 DEG C, pressure be
26min is handled under conditions of 2MPa, filter liquor is collected and obtains material b;
(3) 4 parts of Buckwheat Flower, 2 parts of honeysuckle, 1 part of Herba Andrographitis, 2 parts of cordate houttuynia, 1 part of radix scutellariae, smalt are chosen according to parts by weight
2 parts of leaf, 1 part of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood it is scrubbed after, add 60 DEG C
Water, add milk of lime regulation pH=7.5-8, keep pH it is constant under conditions of boil 20min, borax is then added, at 95 DEG C
Half an hour filtering is incubated, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 25 DEG C, stands and separates out crystallization, separates institute
The crystallization obtained is material c;Wherein, the mass concentration of the borax is 0.35%;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 150MPa, flow velocity 190ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 20 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 35min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Embodiment 2
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 30 parts of 15 parts of 40 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 8mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 12g/100g dosage, is handled under conditions of pressure is 190MPa
10min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 25h is cultivated at 25 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 10min, adjustment is dense
Spend for 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the bar that temperature is 28 DEG C, hunting speed is 200r/min
35min is vibrated under part, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 2:0.3:0.05:0.03:3:1000 mass ratio is mixed;
(2) 10 parts of perilla leaf, 20 parts of konjaku, 8 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 3mm to be broken to granularity, is put in after mixing in the cold water of its 3 times of quality, and 45min is soaked under 15KHz sound wave environment and is mixed
Compound b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 135 DEG C, pressure be
30min is handled under conditions of 3MPa, filter liquor is collected and obtains material b;
(3) 6 parts of Buckwheat Flower, 3 parts of honeysuckle, 2 parts of Herba Andrographitis, 3 parts of cordate houttuynia, 2 parts of radix scutellariae, smalt are chosen according to parts by weight
3 parts of leaf, 2 parts of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood it is scrubbed after, add 70 DEG C
Water, add milk of lime regulation pH=7.5-8, keep pH it is constant under conditions of boil 22min, borax is then added, at 100 DEG C
Half an hour filtering is incubated, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 28 DEG C, stands and separates out crystallization, separates institute
The crystallization obtained is material c;Wherein, the mass concentration of the borax is 0.45%;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 200MPa, flow velocity 260ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 40 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 40min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Embodiment 3
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 28 parts of 12 parts of 38 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 7mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 11g/100g dosage, is handled under conditions of pressure is 180MPa
9min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 24h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 9min, adjust concentration
For 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 27 DEG C, hunting speed is 180r/min
Lower vibration 32min, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 1.2:0.25:0.04:0.025:2.5:1000 mass ratio is mixed;
(2) 9 parts of perilla leaf, 18 parts of konjaku, 6 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 2.5mm to be broken to granularity, is put in after mixing in the cold water of its 2.3 times of quality, and 42min is soaked under 12KHz sound wave environment and is obtained
To mixture b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 130 DEG C, pressure
To handle 28min under conditions of 2.5MPa, collect filter liquor and obtain material b;
(3) 5 parts of Buckwheat Flower, 2.5 parts of honeysuckle, 1.2 parts of Herba Andrographitis, 2.8 parts of cordate houttuynia, radix scutellariae are chosen according to parts by weight
1.5 parts, 2.7 parts of folium isatidis, 1.6 parts of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood pass through
After washing, 65 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 21min under conditions of pH is constant, then
Borax is added, half an hour filtering is incubated at 98 DEG C, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 27 DEG C, is stood
Crystallization is separated out, separating obtained crystallization is material c;Wherein, the mass concentration of the borax is 0.42%;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 160MPa, flow velocity 230ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 30 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 38min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Comparative example 1
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) section for being used as raw material, orange pericarp being cut into 7mm without 28 parts of the orange pericarp to go mouldy is chosen according to parts by weight
Mixture a is obtained after section mixing, mixture a is put into superhigh-voltage homogenizing machine, is handled under conditions of pressure is 180MPa
9min, obtain material a;
(2) 9 parts of perilla leaf, 18 parts of konjaku, 6 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 2.5mm to be broken to granularity, is put in after mixing in the cold water of its 2.3 times of quality, and 42min is soaked under 12KHz sound wave environment and is obtained
To mixture b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 130 DEG C, pressure
To handle 28min under conditions of 2.5MPa, collect filter liquor and obtain material b;
(3) 5 parts of Buckwheat Flower, 2.5 parts of honeysuckle, 1.2 parts of Herba Andrographitis, 2.8 parts of cordate houttuynia, radix scutellariae are chosen according to parts by weight
1.5 parts, 2.7 parts of folium isatidis, 1.6 parts of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood pass through
After washing, 65 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 21min under conditions of pH is constant, then
Borax is added, half an hour filtering is incubated at 98 DEG C, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 27 DEG C, is stood
Crystallization is separated out, separating obtained crystallization is material c;Wherein, the mass concentration of the borax is 0.42%;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 160MPa, flow velocity 230ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 30 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 38min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Comparative example 2
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 28 parts of 12 parts of 38 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 7mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 11g/100g dosage, is handled under conditions of pressure is 180MPa
9min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 24h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 9min, adjust concentration
For 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 27 DEG C, hunting speed is 180r/min
Lower vibration 32min, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 1.2:0.25:0.04:0.025:2.5:1000 mass ratio is mixed;
(2) according to parts by weight choose 9 parts of perilla leaf, it is 2.5mm that perilla leaf is crushed into granularity, be put in after mixing its 2.3
In the cold water of times quality, under 12KHz sound wave environment soak 42min and obtain liquid and obtain material b;
(3) 5 parts of Buckwheat Flower, 2.5 parts of honeysuckle, 1.2 parts of Herba Andrographitis, 2.8 parts of cordate houttuynia, radix scutellariae are chosen according to parts by weight
1.5 parts, 2.7 parts of folium isatidis, 1.6 parts of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood pass through
After washing, 65 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 21min under conditions of pH is constant, then
Borax is added, half an hour filtering is incubated at 98 DEG C, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 27 DEG C, is stood
Crystallization is separated out, separating obtained crystallization is material c;Wherein, the mass concentration of the borax is 0.42%;
(4) step (1) the material a, step (2) the material b, step (3) the material c mix to be put into high pressure micro-
Nano-dispersed processing is carried out in jet nano-dispersed machine, obtains passion fruit antistaling agent;The high pressure microjet nano-dispersed processing
Pressure be 160MPa, flow velocity 230ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 30 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 38min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Comparative example 3
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 28 parts of 12 parts of 38 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 7mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 11g/100g dosage, is handled under conditions of pressure is 180MPa
9min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 24h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 9min, adjust concentration
For 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 27 DEG C, hunting speed is 180r/min
Lower vibration 32min, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 1.2:0.25:0.04:0.025:2.5:1000 mass ratio is mixed;
(2) 9 parts of perilla leaf, 18 parts of konjaku, 6 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 2.5mm to be broken to granularity, is put in after mixing in the cold water of its 2.3 times of quality, and 42min is soaked under 12KHz sound wave environment and is obtained
To mixture b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 130 DEG C, pressure
To handle 28min under conditions of 2.5MPa, collect filter liquor and obtain material b;
(3) step (1) the material a, step (2) the material b are mixed and be put into high pressure microjet nano-dispersed machine
Nano-dispersed processing is carried out, obtains passion fruit antistaling agent;The pressure of the high pressure microjet nano-dispersed processing is 160MPa, stream
Speed is 230ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 30 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 38min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Comparative example 4
A kind of preparation method of passion fruit antistaling agent, comprises the following steps:
(1) chosen according to parts by weight without 28 parts of 12 parts of 38 parts of passion fruit shell, grape fruit pericarp, the orange pericarp to go mouldy
As raw material, mixture a is obtained after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 7mm section mixing, will be mixed
Compound a is put into superhigh-voltage homogenizing machine, and active liquid is added by 11g/100g dosage, is handled under conditions of pressure is 180MPa
9min, obtain material a;
Wherein, described active liquid is prepared by following methods:Aspergillus niger strain is forwarded to potato dextrose agar training
Support in base, 24h is cultivated at 24 DEG C, then the spore of cultured bacterial strain is put into conical flask, shake 9min, adjust concentration
For 1.8 × 107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 27 DEG C, hunting speed is 180r/min
Lower vibration 32min, obtains active liquid;
Described nutrient solution includes following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, grape
Sugar, water are according to 1.2:0.25:0.04:0.025:2.5:1000 mass ratio is mixed;
(2) 9 parts of perilla leaf, 18 parts of konjaku, 6 parts of corn stigma are chosen according to parts by weight, by perilla leaf, konjaku, corn stigma powder
It is 2.5mm to be broken to granularity, is put in after mixing in the cold water of its 2.3 times of quality, and 42min is soaked under 12KHz sound wave environment and is obtained
To mixture b, mixture b is put into more stainless steel extractors, using subcritical extraction method, in temperature be 130 DEG C, pressure
To handle 28min under conditions of 2.5MPa, collect filter liquor and obtain material b;
(3) 5 parts of Buckwheat Flower, 2.5 parts of honeysuckle, 1.2 parts of Herba Andrographitis, 2.8 parts of cordate houttuynia, radix scutellariae are chosen according to parts by weight
1.5 parts, 2.7 parts of folium isatidis, 1.6 parts of oriental wormwood, by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood pass through
After washing, 65 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 21min under conditions of pH is constant, then
Borax is added, half an hour filtering is incubated at 98 DEG C, hydrochloric acid is added into filtrate and is adjusted to pH=6-7, is subsequently cooled to 27 DEG C, is stood
Crystallization is separated out, separating obtained crystallization is material c;Wherein, the mass concentration of the borax is 0.42%;
(4) step (1) the material a, step (2) the material b, step (3) the material c are mixed to get passion fruit
Antistaling agent;The pressure of the high pressure microjet nano-dispersed processing is 160MPa, flow velocity 230ml/min.
The application process of passion fruit antistaling agent, the antistaling agent are used for the fresh-keeping storage of passion fruit, are to the antistaling agent
Used after the middle water for adding 30 times is well mixed;Its application method is:By passion fruit surface clean it is clean after dry, be put in
Taken out at ambient temperature after middle standing 38min in antistaling agent, then low temperature drying to surface is without obvious water stain, you can is stored up
Deposit.
Experimental example
Sensory test
Experiment is divided into 7 groups, and passion fruit antistaling agent is used to protect by each group according to embodiment 1-3 and comparative example 1-4 application processes
Fresh 100 passion fruits, observation, record preserve second month fruit browning, mildew situation, and standards of grading are shown in Table 1, and appraisal result is shown in
Table 2.
The fresh-keeping effect standards of grading of the passion fruit antistaling agent of table 1
Evaluation criterion | Score |
Fresh intact, no foxiness or waterlogging spot, or speck area are less than 1/4, and flesh color, flavor are normal, non-mildew | One-level fruit |
Between 1/4-1/2, flesh color, flavor are normal for foxiness or waterlogging spot area and fruit area ratio, non-mildew | Two level fruit |
Between 1/2-3/4, flesh color, flavor are slightly poor for foxiness or waterlogging spot area and fruit area ratio, slight mildew | Three-level fruit |
Foxiness or waterlogging spot area are more than 3/4 with fruit area ratio, and flesh color, flavor are poor, mildew | Level Four fruit |
Fruit rots completely | Pyatyi fruit |
The fresh-keeping effect of the passion fruit antistaling agent of table 2
Embodiment 1 | Embodiment 2 | Embodiment 3 | Comparative example 1 | Comparative example 2 | Comparative example 3 | Comparative example 4 | |
Score | One-level fruit | One-level fruit | One-level fruit | Level Four fruit | Three-level fruit | Level Four fruit | Two level fruit |
Browning rate (%) | 0 | 0 | 0 | 80 | 60 | 85 | 20 |
Weight-loss ratio (%) | 98.9 | 99.2 | 99.5 | 22.8 | 38.9 | 18.3 | 75.2 |
Wherein, the fruit number that generation brown stain and cortex degenerate after browning rate=storage/total fruit number × 100%
Fruit gross mass × 100% before fruit gross mass/storage after weight-loss ratio=storage
The result of table 2 shows:Embodiment 1-3 passion fruit antistaling agent is interior during the experiment of two months can still to keep fresh
State, illustrate the passion fruit antistaling agent commonly using the present invention, phenomena such as can preventing passion fruit fruit from producing weightless, shrinkage, dimension
The outward appearance of passion fruit and its quality of pulp are held, extends the pot-life of passion fruit.
Although above the present invention is described in detail with a general description of the specific embodiments,
On the basis of the present invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Cause
This, these modifications or improvements, belong to the scope of protection of present invention without departing from theon the basis of the spirit of the present invention.
Claims (6)
1. a kind of preparation method of passion fruit antistaling agent, it is characterised in that comprise the following steps:
(1) chosen according to parts by weight without the passion fruit shell 35-40 part, grape fruit pericarp 10-15 parts, orange pericarp 25- gone mouldy
30 parts of conduct raw material, mixed after passion fruit shell, grape fruit pericarp, orange pericarp to be cut into 5-8mm section mixing
Thing a, mixture a is put into superhigh-voltage homogenizing machine, active liquid is added by 10-12g/100g dosage, in pressure be 170-
5-10min is handled under conditions of 190MPa, obtains material a;
(2) perilla leaf 8-10 parts, konjaku 15-20 parts, corn stigma 5-8 parts are chosen according to parts by weight, by perilla leaf, konjaku, corn
It is 2-3mm that granularity, which must be crushed to, is put in after mixing in the cold water of its 2-3 times of quality, is soaked under 10-15KHz sound wave environment
40-45min obtains mixture b, and mixture b is put into more stainless steel extractors, using subcritical extraction method, is in temperature
125-135 DEG C, pressure handle 26-30min under conditions of being 2-3MPa, collect filter liquor and obtain material b;
(3) Buckwheat Flower 4-6 parts, honeysuckle 2-3 parts, Herba Andrographitis 1-2 parts, cordate houttuynia 2-3 parts, radix scutellariae 1-2 are chosen according to parts by weight
Part, folium isatidis 2-3 parts, oriental wormwood 1-2 parts are scrubbed by Buckwheat Flower, honeysuckle, Herba Andrographitis, cordate houttuynia, radix scutellariae, folium isatidis, oriental wormwood
Afterwards, 60-70 DEG C of water is added, adds milk of lime regulation pH=7.5-8, keeps boiling 20-22min under conditions of pH is constant, so
After add borax, 95-100 DEG C be incubated half an hour filtering, into filtrate add hydrochloric acid be adjusted to pH=6-7, be subsequently cooled to 25-
28 DEG C, stand and separate out crystallization, separating obtained crystallization is material c;
(4) step (1) the material a, step (2) the material b, step (3) the material c are mixed and is put into high pressure microjet
Nano-dispersed processing is carried out in nano-dispersed machine, obtains passion fruit antistaling agent;The pressure of the high pressure microjet nano-dispersed processing
Power is 150-200MPa, flow velocity 190-260ml/min.
2. the preparation method of a kind of passion fruit antistaling agent according to claim 1, it is characterised in that described in step (1)
Active liquid prepared by following methods:Aspergillus niger strain is forwarded in potato dextrose agar, at 23-25 DEG C
Cultivate 20-25h, then the spore of cultured bacterial strain is put into conical flask, shake 8-10min, adjustment concentration be 1.8 ×
107Individual/ml, nutrient solution is added into conical flask, in the condition that temperature is 26-28 DEG C, hunting speed is 160-200r/min
Lower vibration 30-35min, obtains active liquid.
A kind of 3. preparation method of passion fruit antistaling agent according to claim 2, it is characterised in that described nutrient solution bag
Include following raw material:Ammonium sulfate, potassium dihydrogen phosphate, copper sulphate, magnesium sulfate, sodium chloride, glucose, water are according to 1-2:0.2-0.3:
0.03-0.05:0.02-0.03:2-3:1000 mass ratio is mixed.
4. a kind of preparation method of passion fruit antistaling agent according to claim 1, it is characterised in that described in step (3)
The mass concentration of borax is 0.35-0.45%.
5. the passion fruit antistaling agent that the preparation method described in claim any one of 1-4 obtains.
A kind of 6. application process of passion fruit antistaling agent as claimed in claim 5, it is characterised in that:Described antistaling agent is used for
The fresh-keeping storage of passion fruit, the antistaling agent are used after the water of 20-40 times of addition thereto is well mixed;Its user
Method is:By passion fruit surface clean it is clean after dry, taken out after being put in antistaling agent middle standing 35-40min at ambient temperature,
Then low temperature drying can be stored to surface without obvious water stain.
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CN108094515A (en) * | 2017-12-15 | 2018-06-01 | 柳州市柳科科技有限公司 | A kind of mulberries antistaling agent and preparation method thereof |
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CN111869727B (en) * | 2020-07-16 | 2023-05-02 | 广西壮族自治区农业科学院 | Nanometer material formula for passion fruit fresh-keeping and preparation method thereof |
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CN114504022B (en) * | 2022-02-28 | 2024-01-23 | 贵阳学院 | Passion fruit preservative as well as preparation method and application thereof |
CN115005265A (en) * | 2022-07-21 | 2022-09-06 | 青岛新万福食品有限公司 | Fruit and vegetable fermented extract and application thereof in meat product preservation |
CN115005265B (en) * | 2022-07-21 | 2023-08-25 | 青岛新万福食品有限公司 | Fruit and vegetable fermentation extract and application thereof in fresh-keeping of meat products |
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