CN107409534A - A kind of authentication method of cotton alkali resistance - Google Patents
A kind of authentication method of cotton alkali resistance Download PDFInfo
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- CN107409534A CN107409534A CN201710803947.XA CN201710803947A CN107409534A CN 107409534 A CN107409534 A CN 107409534A CN 201710803947 A CN201710803947 A CN 201710803947A CN 107409534 A CN107409534 A CN 107409534A
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- alkaline
- radicle
- cotton
- resisting
- germplasms
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
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- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The present invention provides a kind of method of the alkaline-resisting identification of Cotton Germplasms, including：Cotton seeds lint is handled；Successively with hydrogen peroxide dipping, aseptic water washing；Sterilized water Steeping and budding；(2) alkaline stress processing is carried out to " showing money or valuables one carries unintentionally " seed, clear water compares, and investigates the radicle survival rate under two kinds of processing, carries out alkali resistance overall merit；In 9807, as identification control material calculating radicle relative survival, obtain the alkaline-resisting evaluation criterion of Cotton Germplasms；(3) Cotton Germplasms to be identified are pre-processed, alkaline stress processing, investigates its radicle survival rate, calculate radicle relative survival, according to the alkaline-resisting evaluation criterion of Cotton Germplasms, identify the alkaline-resisting rank of Cotton Germplasms.Alkaline stress processing condition be：25mM Na2CO3Alkalescence condition under handle 5d.The present invention carries out indoor alkali resistance identification to Cotton Germplasms using stress sproutung method, and the cycle is 5 days, and the test period is short, limit by outside climatic condition, is not required to cost lot of experiments expense, establishes a kind of economic, alkaline-resisting authentication method of cotton easily and fast.
Description
Technical field
The invention belongs to the variety evaluation technology in agriculture field, and in particular to a kind of alkaline-resisting identification side of cotton germination period
Method, suitable for the screening and evaluation of the alkaline-resisting germ plasm resource of cotton.
Background technology
The salinization of soil be always influence growth and development of plants, reduce crop yield an important factor for.According to joint state religion
Section's text tissue (UNESCO) and food and agricultural organization (FAO) incomplete statistics, whole world saline alkali land area is about 9.54 hundred million hm2(Zhang
T.L, 2000), with irrational crop rotation and blindness or chemical fertilizer excessively is applied, exacerbates Pedotransfer function.There is research
Show, when the soluble salt content of soil is more than 0.3%, to will result in the salinization of soil.Saline-alkali soil is according to whether have salic
It can be divided into solonchak, alkaline earth with natric horizon.The principal component of lyotropic salt contained by two kinds of great soil groups is different, solonchak with neutral salt (NaCl,
Na2SO4) based on, alkaline earth is with basic salt (NaHCO3、Na2CO3) based on.
Cotton studies more deep, the research to cotton alkali resistance as salt-soda soil pioneer crop, forefathers to salt tolerance of cotton
It is less.The alkaline-resisting kind of cotton is cultivated, it is significant to further improved soil salt-soda soil, expansion cultivable land area.
Cotton has 6300 parts of germ plasm resources, and abundant material is provided for the alkaline-resisting identification of cotton and screening, alkaline-resisting breed of variety.Cotton Germplasms
Aboundresources, therefore establish a kind of fast and easy, be adapted to the method for the extensive alkaline-resisting identification of cotton material most important.
The content of the invention
It is an object of the invention to provide a kind of method and evaluation criterion of the alkaline-resisting identification of Cotton Germplasms easily and fast.
The method of the alkaline-resisting identification of Cotton Germplasms provided by the present invention, comprises the steps：
(1) cotton seeds are subjected to lint processing；Carry out disinfection processing to the cotton seeds after lint；Again with sterile water logging
Bubble carries out vernalization, obtains " showing money or valuables one carries unintentionally " seed；
(2) alkaline stress processing being carried out to " showing money or valuables one carries unintentionally " seed, clear water compares, and investigates the radicle survival rate under two kinds of processing,
Carry out alkali resistance overall merit；Wherein, in 9807 as identification control material, calculates radicle relative survival, obtains cotton
The alkaline-resisting evaluation criterion of germplasm；
(3) use the method in step (1) to pre-process Cotton Germplasms to be identified, pretreated seed is carried out
Alkaline stress processing, clear water compare, and investigate its radicle survival rate, and in 9807, as identification control material, it is relative to calculate radicle
Survival rate, the alkaline-resisting evaluation criterion of Cotton Germplasms in step (2), identify the alkaline-resisting ranks of Cotton Germplasms to be identified.
In above method step (1), the concentrated sulfuric acid can be used to carry out lint processing to cotton seeds.
The operation disinfected can be：Successively with hydrogen peroxide dipping, aseptic water washing.
The hydrogen peroxide is mass concentration 20%-30% hydrogen peroxide.
The each 1min of aseptic water washing, it can carry out repeatedly, concretely 5 times.
The time of the sterilized water immersion is 10-24 hours, concretely 12h.
In above method step (2), the condition of the alkaline stress processing is：25mM Na2CO3Alkalescence condition under handle
5d。
Radicle survival rate=alkaline stress treatment group survival rate/clear water composition motility rate × 100%；
Radicle relative survival=detected materials radicle survival rate × 50%/control material radicle survival rate
The alkaline-resisting evaluation criterion of Cotton Germplasms is：When radicle relative survival>When 90.0%, the Cotton Germplasms are " high
It is anti-" alkaline-resisting rank；When radicle relative survival is between 75.0%-89.9%, the Cotton Germplasms are " anti-" alkaline-resisting rank；When
When radicle relative survival is between 50.0%-74.9%, the Cotton Germplasms are " resistance to " alkaline-resisting rank；When radicle relative survival
In 0.0%-49.9%, the Cotton Germplasms are " intolerant to " alkaline-resisting rank.
The present invention carries out indoor alkali resistance identification to Cotton Germplasms using stress sproutung method, and the test for identification cycle is 5 days, examination
It is short to test the cycle, and is not limited by outside climatic condition, is not required to spend lot of experiments expense, tentatively establishes a kind of relatively inexpensive
, the alkaline-resisting authentication method of cotton easily and fast.
Brief description of the drawings
Fig. 1 is influence of the various concentrations different time alkali process to cotton seed germination.Wherein, a is to sprout feelings on the 3rd day
Condition；B is the 5th day sprouting situation；C is the 7th day sprouting situation；D is 25mM Na2CO3, 5d sprouting phenotypes；A, B, C are respectively
GK50, Henan 2067, Ji 169.
Fig. 2 is whether radicle survives criteria for classifying diagram.The character criteria for classifying：Survive：Radicle is intact, without rotten；It is aggrieved：
Radicle has different degrees of rotten；Aggrieved grade classification is 3 grades：1 grade：The rotten root long degree of radicle accounts for total length<1/3；2 grades：Radicle
Rotten root long degree accounts for total length>1/3,<2/3；3 grades：The rotten root long degree of radicle accounts for total length>2/3.
Embodiment
Below by specific embodiment, the present invention will be described, but the invention is not limited in this.
Experimental method used in following embodiments is conventional method unless otherwise specified；Institute in following embodiments
Reagent, material etc., unless otherwise specified, commercially obtain.
Embodiment
(1) Seeds preprocess：
Cotton seeds are subjected to lint processing with the concentrated sulfuric acid；
The consistent seed of maturity is selected, successively with 20%-30% hydrogen peroxide dipping 3-4 hours, aseptic water washing 5 times
(each 1min) carries out disinfection processing；
Vernalization is carried out with sterilized water immersion seed 12h.
(2) alkaline stress conditional filtering
Material：Conventional cotton variety (GK50, Henan 2067, Ji 169)
Select in the same size, " showing money or valuables one carries unintentionally " 100, seed (3 repetitions)；
Vertical double-layer filter paper method：Filter paper is laid on plastic plate, with (10,25,40mM) Na2CO3Filter paper is soaked, clearly
Water does blank control, seed growth point is placed on filter paper to same direction, lateral separation 6cm between seed, between longitudinal direction
Every 5cm, filter paper is laterally rolled along seed position, by seed volume among filter paper so that filter paper forms straight-tube shape.Straight-tube shape is filtered
Paper is placed in beaker vertically, and beaker bottom adds appropriate aqueous slkali, keeps filter paper to moisten all the time.
Growth conditions：Beaker is put into artificial incubator, 28 DEG C, 24h dark processings 3,5,7d.Observe seed sprouting situation
(such as Fig. 1), find 25mM Na2CO3The aggrieved phenotype of radicle is obvious and not lethal when handling 5d, selects 25mM Na2CO3, 5d conducts
Whether alkaline stress treatment conditions, investigation radicle survive the characteristic index of (criteria for classifying such as Fig. 2) as screening.
(3) 31 parts of germplasm materials alkali resistance evaluations of cotton
31 parts of material screenings：Seeds preprocess (same to step (1)), seed preserve by degeneration-resistant identification problem, germplasm materials
It is mainly derived from the kind that Huanghe valley cotton region is widely applied, and the material of this problem autonomous innovation.
Table 1：Test material title and source
Continued
25mM Na2CO3, 5d, vertical double-layer filter paper method, radicle survival rate is investigated, as a result counts as follows：
Cotton radicle survival rate is investigated under the different disposal of table 2
The two-way analysis of variance of table 3
Variance analysis shows that area's group difference is not notable, i.e. repeatability is preferable between three district's groups；Difference is extremely aobvious between processing
Write, illustrate that alkaline stress processing has obvious inhibiting effect to seed base-root survival rate；Interaction presence is extremely notable between kind × processing
Otherness, illustrate to have differences in alkali resistance between different cultivars.
Alkali resistance sequence is carried out according to radicle survival rate
Radicle survival rate/%=test groups survival rate/control group survival rate × 100
The alkali resistance size of table 4 is evaluated
(4) qualification result is evaluated
According to alkaline-resisting evaluation result, 9807 controls identified as alkali resistance in selecting in 31 parts of materials alkali resistance most strong
Material, calculates the relative radicle survival rate of each material, and material is divided into following level Four：
With respect to the radicle survival rate of the control material of each material radicle survival rates of radicle survival rate/%=× 50/
The alkali resistance grade classification of table 5
According to above-mentioned grading standard, the alkali resistance evaluation result of 31 parts of materials is obtained
Claims (5)
1. a kind of method of the alkaline-resisting identification of Cotton Germplasms, comprises the steps：
(1) cotton seeds are subjected to lint processing；Carry out disinfection processing to the cotton seeds after lint；Again with sterilized water soak into
Row vernalization, obtain " showing money or valuables one carries unintentionally " seed；
(2) alkaline stress processing is carried out to " showing money or valuables one carries unintentionally " seed, clear water compares, and investigates the radicle survival rate under two kinds of processing, carries out
Alkali resistance overall merit；Wherein, in 9807 as identification control material, calculates radicle relative survival, obtains Cotton Germplasms
Alkaline-resisting evaluation criterion；
(3) use the method in step (1) to pre-process Cotton Germplasms to be identified, the alkali side of body is carried out to pretreated seed
Compel processing, clear water compares, and investigates its radicle survival rate, and in 9807, as identification control material, calculate radicle and survive relatively
Rate, the alkaline-resisting evaluation criterion of Cotton Germplasms in step (2), identify the alkaline-resisting ranks of Cotton Germplasms to be identified.
2. according to the method for claim 1, it is characterised in that：In step (1), cotton seeds are taken off using the concentrated sulfuric acid
Suede processing；
The operation disinfected is：Successively with hydrogen peroxide dipping, aseptic water washing；
The hydrogen peroxide is mass concentration 20%-30% hydrogen peroxide；
The each 1min of aseptic water washing, carry out multiple；
The time of the sterilized water immersion is 10-24 hours.
3. method according to claim 1 or 2, it is characterised in that：In step (2), the condition of the alkaline stress processing is：
25mM Na2CO3Alkalescence condition under handle 5d.
4. according to the method any one of claim 1-3, it is characterised in that：In step (2), the radicle survival rate=alkali side of body
Compel treatment group survival rate/clear water composition motility rate × 100%；
Radicle relative survival=detected materials radicle survival rate × 50%/control material radicle survival rate.
5. according to the method any one of claim 1-4, it is characterised in that：In step (2), the Cotton Germplasms are alkaline-resisting
Evaluation criterion is：When radicle relative survival>When 90.0%, the Cotton Germplasms are " height is anti-" alkaline-resisting rank；When radicle relative into
When motility rate is between 75.0%-89.9%, the Cotton Germplasms are " anti-" alkaline-resisting rank；When radicle relative survival is in 50.0%-
When between 74.9%, the Cotton Germplasms are " resistance to " alkaline-resisting rank；When radicle relative survival is in 0.0%-49.9%, the cotton
Germplasm is " intolerant to " alkaline-resisting rank.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710803947.XA CN107409534B (en) | 2017-09-08 | 2017-09-08 | Method for identifying alkali resistance of cotton |
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| CN201710803947.XA CN107409534B (en) | 2017-09-08 | 2017-09-08 | Method for identifying alkali resistance of cotton |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111656905A (en) * | 2020-07-06 | 2020-09-15 | 中国农业科学院棉花研究所 | Na-resistant cotton2SO4Method of (3) identification |
| CN111855920A (en) * | 2020-08-05 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying alkali stress degree of cotton |
| CN115399106A (en) * | 2021-05-28 | 2022-11-29 | 中国农业科学院棉花研究所 | Evaluation of Na resistance of long staple cotton 2 CO 3 Sexual method |
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| CN103843491A (en) * | 2014-03-25 | 2014-06-11 | 山西省农业科学院棉花研究所 | Method for quickly detecting salt tolerance of cotton variety |
| CN105230179A (en) * | 2015-09-28 | 2016-01-13 | 河北省农林科学院旱作农业研究所 | Multi-stress synchronous stress breeding method for cotton breeding |
| CN105874967A (en) * | 2016-04-20 | 2016-08-24 | 运城学院 | Method for identifying salt-stressed resistance in cotton seeds |
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| CN105874967A (en) * | 2016-04-20 | 2016-08-24 | 运城学院 | Method for identifying salt-stressed resistance in cotton seeds |
| CN106069243A (en) * | 2016-06-06 | 2016-11-09 | 中国农业科学院棉花研究所 | Semi-wild cotton germination period is combined saline and alkaline Resistance Identification method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111656905A (en) * | 2020-07-06 | 2020-09-15 | 中国农业科学院棉花研究所 | Na-resistant cotton2SO4Method of (3) identification |
| CN111855920A (en) * | 2020-08-05 | 2020-10-30 | 中国农业科学院棉花研究所 | Method for identifying alkali stress degree of cotton |
| CN115399106A (en) * | 2021-05-28 | 2022-11-29 | 中国农业科学院棉花研究所 | Evaluation of Na resistance of long staple cotton 2 CO 3 Sexual method |
| CN115399106B (en) * | 2021-05-28 | 2023-08-11 | 中国农业科学院棉花研究所 | Evaluation of Na resistance of Long stapled cotton 2 CO 3 Sex method |
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| CN107409534B (en) | 2020-04-28 |
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