CN107406883B - 多重检测直接来自含有血液的样品的核酸靶标 - Google Patents

多重检测直接来自含有血液的样品的核酸靶标 Download PDF

Info

Publication number
CN107406883B
CN107406883B CN201680013510.XA CN201680013510A CN107406883B CN 107406883 B CN107406883 B CN 107406883B CN 201680013510 A CN201680013510 A CN 201680013510A CN 107406883 B CN107406883 B CN 107406883B
Authority
CN
China
Prior art keywords
blood
reaction
nucleic acid
excitation
sample
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201680013510.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN107406883A (zh
Inventor
N·纳萨雷特
D·埃奇
A·泰勒
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BG Research Ltd
Original Assignee
BG Research Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BG Research Ltd filed Critical BG Research Ltd
Publication of CN107406883A publication Critical patent/CN107406883A/zh
Application granted granted Critical
Publication of CN107406883B publication Critical patent/CN107406883B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CN201680013510.XA 2015-03-05 2016-03-04 多重检测直接来自含有血液的样品的核酸靶标 Active CN107406883B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB201503775A GB201503775D0 (en) 2015-03-05 2015-03-05 Multiplexed detection of nucleic acid targets directly from samples containing blood
GB1503775.7 2015-03-05
PCT/GB2016/000046 WO2016139443A1 (en) 2015-03-05 2016-03-04 Multiplexed detection of nucleic acid targets directly from samples containing blood

Publications (2)

Publication Number Publication Date
CN107406883A CN107406883A (zh) 2017-11-28
CN107406883B true CN107406883B (zh) 2021-06-22

Family

ID=52998469

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201680013510.XA Active CN107406883B (zh) 2015-03-05 2016-03-04 多重检测直接来自含有血液的样品的核酸靶标

Country Status (5)

Country Link
EP (1) EP3334533B1 (enExample)
JP (2) JP2018507694A (enExample)
CN (1) CN107406883B (enExample)
GB (1) GB201503775D0 (enExample)
WO (1) WO2016139443A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201806762D0 (en) 2018-04-25 2018-06-06 Bg Res Ltd Improved processes for performing direct detection
GB201812428D0 (en) 2018-07-31 2018-09-12 Bg Res Ltd Improved method for pathogen detection
CN112782395B (zh) * 2020-12-30 2021-11-09 北京中科生仪科技有限公司 一种pcr检测仪及其方法
JP7393070B1 (ja) * 2023-08-04 2023-12-06 株式会社ゴーフォトン Ｐｃｒ方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101624629A (zh) * 2009-07-24 2010-01-13 上海浩源生物科技有限公司 单管中多重靶核酸的pcr检测方法及其试剂盒

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007521016A (ja) * 2003-10-16 2007-08-02 サード・ウェーブ・テクノロジーズ・インク 体液における直接的な核酸検出
WO2006050499A2 (en) * 2004-11-03 2006-05-11 Third Wave Technologies, Inc. Single step detection assay
US20070098594A1 (en) * 2005-11-03 2007-05-03 Roche Molecular Systems, Inc. Analytical multi-spectral optical detection system
GB0718250D0 (en) 2007-08-29 2007-10-31 B G Res Ltd Improvements in and relating to reaction apparatus
US20120183965A1 (en) * 2009-06-15 2012-07-19 David Ward Nucleic acid detection
GB201009998D0 (en) 2010-06-15 2010-07-21 Bg Res Cell disruption
WO2014059281A1 (en) * 2012-10-11 2014-04-17 The Regents Of The University Of Michigan Fluorescence polarization assay for detecting high molecular weight molecules in biological fluids
GB201401584D0 (en) 2014-01-29 2014-03-19 Bg Res Ltd Intelligent detection of biological entities

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101624629A (zh) * 2009-07-24 2010-01-13 上海浩源生物科技有限公司 单管中多重靶核酸的pcr检测方法及其试剂盒

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Multiphoton excitation of fluorescent DNA base analogs;Katilius Evaldas;《JOURNAL OF BIOMEDICAL OPTICS》;20060101;第11卷(第4期);第044004-4页 *
リMx3000P/Mx3005Pアルタイム定量PCRシステム;安捷伦;《Mx3000P/Mx3005Pアルタイム定量PCRシステム》;20091215;第1-14页 *
人血液荧光的光谱特性及其物质来源;李不洪;《光谱学与光谱分析》;20060731;第26卷(第7期);第l310—1313页 *
可见光诱导血液荧光光谱研究;高淑梅;《中国优秀博硕士学位论文全文数据库 (博士)基础科学辑》;20030315(第 01 期);第45页 *
安捷伦.リMx3000P/Mx3005Pアルタイム定量PCRシステム.《Mx3000P/Mx3005Pアルタイム定量PCRシステム》.2009,第1-14页. *

Also Published As

Publication number Publication date
JP6877605B2 (ja) 2021-05-26
JP2018507694A (ja) 2018-03-22
JP2020110153A (ja) 2020-07-27
EP3334533A1 (en) 2018-06-20
CN107406883A (zh) 2017-11-28
EP3334533B1 (en) 2021-04-21
WO2016139443A1 (en) 2016-09-09
GB201503775D0 (en) 2015-04-22
HK1244514A1 (zh) 2018-08-10

Similar Documents

Publication Publication Date Title
Chan Recent advances in laser tweezers Raman spectroscopy (LTRS) for label‐free analysis of single cells
Huser et al. Raman spectroscopy for physiological investigations of tissues and cells
JP6877605B2 (ja) 血液を含むサンプルからの直接の核酸標的の多重検出
JP2021192044A (ja) 時間分解発光を使用して核酸の配列を決定するための方法
CN103443290B (zh) 用于评估生物分子特性的系统和方法
US20170247745A1 (en) Multiplex optical detection
US20230129517A1 (en) System and method for tissue viability screening
CN115053118B (zh) 使用特殊化细胞识别进行循环流式细胞术的装置和方法
US10081830B2 (en) Method of detecting nucleic acids, method of optically observing sample and fluorescent substance
WO2013192106A1 (en) Filter architecture for analytical devices
US20240017256A1 (en) An apparatus & method for processing and analysing one or more samples
CN103782157B (zh) 包含胰液的生物试样中的目标粒子的检测方法
US9464329B2 (en) Portable systems and methods for amplifying nucleotides and detecting nucleotide sequences
CN101903761B (zh) 检测系统和方法
HK1244514B (zh) 多重检测直接来自含有血液的样品的核酸靶标
US12391982B2 (en) Optical discrimination apparatus and methods adapted to monitor reactions
RU118064U1 (ru) Устройство для одновременного контроля в реальном масштабе времени множества амплификаций нуклеиновой кислоты
Kim et al. Optical system for multi-channel real-time PCR detection
Chang et al. Fiber-optic multiphoton in vivo flow cytometry
Chang et al. Two-photon in vivo flow cytometry using a fiber probe
KR20230135970A (ko) 형광 집속 렌즈가 포함된 pcr 칩 및 이를 이용한 pcr 진단 기기 및 진단 방법
EP4126368A1 (fr) Lecteur de terrain d'echantillons biologiques traites par amplification isotherme de l'adn mediee par les boucles
KR20220018848A (ko) 미생물 현장 검사를 위한 자가형광 분석 장치 및 그 방법
HK1230667B (zh) 用於评估生物分子特性的系统和方法
De Luca et al. Fluorescence-free biochemical characterization of cells using modulated Raman spectroscopy

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 1244514

Country of ref document: HK

GR01 Patent grant
GR01 Patent grant