CN107384771A - A kind of genetic polymorphism detection kit - Google Patents
A kind of genetic polymorphism detection kit Download PDFInfo
- Publication number
- CN107384771A CN107384771A CN201710753835.8A CN201710753835A CN107384771A CN 107384771 A CN107384771 A CN 107384771A CN 201710753835 A CN201710753835 A CN 201710753835A CN 107384771 A CN107384771 A CN 107384771A
- Authority
- CN
- China
- Prior art keywords
- test bottle
- box body
- lid
- neck
- layer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of genetic polymorphism detection kit, mainly include box body, lid and test bottle, the box body bottom is provided with backing plate, it is connected at the top of the box body by hinge with lid, the lid leading flank is provided with lockset, the tray interior is provided with sponge cut-off, and it is provided with neck on sponge cut-off, and neck is multiple, the test bottle is arranged on inside neck, the test bottle outer wall is brought into close contact with neck inwall, set at the top of the backing plate fluted, the test bottle bottom and engagement, buckle is provided with the lid medial surface, and Reagent Tube is provided with buckle, the present invention is simple in construction, it is safe to use convenient, the security of test bottle can be effectively improved, and compared to available reagent box small volume, it is simple to operate, it is readily transported and uses.
Description
Technical field
The present invention relates to kit technical field, specially a kind of genetic polymorphism detection kit.
Background technology
Polymorphism refers in a biocenose, while and is frequently present of two or more discontinuous anomalies or base
Because of type or allele, also known as genetic polymorphism or gene pleiomorphism.Intrinsically, the generation of polymorphism is gene water
Variation on flat, typically occur in gene order the not region of encoding proteins and the region without important regulatory function.For
For one individual, gene pleiomorphism base sequence is constant throughout one's life, and is passed on from generation to generation by Mendel's rule.
Biocenose gene pleiomorphism phenomenon is very universal, wherein, structure, expression and the function of human gene, study ratio
It is more deep.Human gene polymorphism had both derived from the difference of repetitive sequence copy number in genome, also from single-copy sequence
Variation, and the conversion or replacement of diallele.By the priority for causing concern and research, 3 major classes are generally divided into：DNA pieces
Segment length polymorphism, repetitive dna sequence polymorphism, SNP.
Mthfr gene polymorphism (C677T) detection method has following several at present：It is the gene as " goldstandard " first
Sequencing, has higher specificity, and can detect greatly flux, but because sequencing instrument is costly, has no idea general
And if detected by third party's service, need higher time cost, preservation and transport and one kind of sample
Challenge.Based on molecular level, report is mostly RECP to the detection method of mthfr gene both at home and abroad so far, has been limited since the method
Property endonuclease digestion processed, electrophoretic analysis, digestion are not exclusively often mistaken for heterozygote, because PCR primer will carry out open pipe behaviour
Make, easily form aerosol, cause the generation of false positive.Electrophoretic analysis needs to prepare polyacrylamide gel, and EB dyeing is time-consuming
Arduously, and there is a chemical hazard.There is the method using MGB probes a few days ago, the method can avoid pollution of uncapping, and have stronger
Specificity, but the synthesis of MGB probes requires higher, and only number companies of the country can synthesize, and expensive discomfort
Conventional inspection is closed, still, the security of existing kit is not high, easily causes the test bottle of tray interior to damage.
The content of the invention
It is an object of the invention to provide a kind of genetic polymorphism detection kit, to solve to propose in above-mentioned background technology
The problem of.
To achieve the above object, the present invention provides following technical scheme：A kind of genetic polymorphism detection kit, including box
Body, lid and test bottle, the box body bottom are provided with backing plate, are connected at the top of the box body by hinge with lid, the box
Lid leading flank is provided with lockset, and the tray interior is provided with sponge cut-off, and is provided with neck on sponge cut-off, and neck is
Multiple, the test bottle is arranged on inside neck, and the test bottle outer wall is brought into close contact with neck inwall, is set at the top of the backing plate
Groove, the test bottle bottom and engagement are equipped with, is provided with buckle on the lid medial surface, and examination is provided with buckle
Agent pipe.
Preferably, layer protective layer is provided with the box body outer wall, and protective layer includes net body, reinforcement and antibacterial
Layer, reinforcement is equipped with the grid cell of the net body, the reinforcement passes through the centre position of grid cell, Duo Gejia
Strengthening tendons be arranged in parallel along the distribution arrangement of grid cell, and the antibiotic layer is coated on net body.
Preferably, the antibiotic layer includes polyurethane foam, alkali-resistant glass fibre screen cloth, carbon fiber layer and phenolic resin
Coating, alkali-resistant glass fibre screen cloth is provided with the polyurethane foam, carbon is provided with the alkali-resistant glass fibre screen cloth
Fibrous layer, phenolic coating is coated with the carbon fiber layer.
Preferably, the test bottle is internally provided with reaction system.
Compared with prior art, the beneficial effects of the invention are as follows：
(1) present invention is simple in construction, safe to use convenient, can effectively improve the security of test bottle, and compared to existing
There is kit small volume, it is simple to operate, it is readily transported and uses.
(2) present invention in, employ and one layer of antimicrobial coating be coated with box body outer wall, have antibacterial, it is wear-resisting, fire-retardant,
Resistance to compression, distortion-free advantage, it is suitable for using under rugged environment, it is ensured that the security and antibacterial of tray interior test bottle
Property.
(3) in the present invention, employ and be equipped with reinforcement on net body, ensure box using reinforcement and net body fits
The security of body.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention.
Fig. 2 is protective layer structure schematic cross-sectional view of the present invention.
Fig. 3 is antibacterial Rotating fields schematic cross-sectional view of the present invention.
In figure：1st, box body；2nd, lid；3rd, test bottle；4th, backing plate；5th, lockset；6th, sponge is separated；7th, Reagent Tube；8th, protect
Layer；9th, net body；10th, reinforcement；11st, antibiotic layer；12nd, polyurethane foam；13rd, alkali-resistant glass fibre screen cloth；14th, carbon fiber
Layer；15th, phenolic coating.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation describes, it is clear that described embodiment is only part of the embodiment of the present invention, rather than whole embodiments.It is based on
Embodiment in the present invention, those of ordinary skill in the art are obtained every other under the premise of creative work is not made
Embodiment, belong to the scope of protection of the invention.
Fig. 1-3 are referred to, the present invention provides a kind of technical scheme：A kind of genetic polymorphism detection kit, including box body
1st, lid 2 and test bottle 3, the bottom of box body 1 are provided with backing plate 4, and the top of box body 1 is connected by hinge with lid 2, the front side of lid 2
Face is provided with lockset 5, and box body 1 is internally provided with sponge cut-off 6, and neck is provided with sponge cut-off 6, and neck be it is multiple,
Test bottle 3 is arranged on inside neck, and the outer wall of test bottle 3 is brought into close contact with neck inwall, and the top of backing plate 4 sets fluted, test
Bottle 3 bottoms and engagement, buckle is provided with the medial surface of lid 2, and Reagent Tube 7 is provided with buckle.
In the present invention, layer protective layer 8 is provided with the outer wall of box body 1, and protective layer 8 includes net body 9, the and of reinforcement 10
Antibiotic layer 11, reinforcement 10 is equipped with the grid cell of net body 9, reinforcement 10 passes through the centre position of grid cell, more
Individual reinforcement 10 be arranged in parallel along the distribution arrangement of grid cell, and antibiotic layer 11 is coated on net body 9；Employ in net originally
Reinforcement is equipped with body, the security of box body is ensured using reinforcement and net body fits.Antibiotic layer 11 includes polyurethane foam
Foam layer 12, alkali-resistant glass fibre screen cloth 13, carbon fiber layer 14 and phenolic coating 15, it is provided with polyurethane foam 12 resistance to
Alkali containing glass fibre screen cloth 13, carbon fiber layer 14 is provided with alkali-resistant glass fibre screen cloth 13, phenolic aldehyde is coated with carbon fiber layer 14
Resinous coat 15；Employ and one layer of antimicrobial coating is coated with box body outer wall, there is antibacterial, wear-resisting, fire-retardant, resistance to compression, preapre for an unfavorable turn of events
The advantages of shape, it is suitable for using under rugged environment, it is ensured that the security and antibiotic property of tray interior test bottle.
In addition, in the present invention, test bottle 3 is internally provided with reaction system.
In summary, the present invention is simple in construction, safe to use convenient, can effectively improve the security of test bottle, and phase
It is simple to operate compared with available reagent box small volume, it is readily transported and uses.
Although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of changes, modification can be carried out to these embodiments, replace without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (4)
1. a kind of genetic polymorphism detection kit, including box body (1), lid (2) and test bottle (3), it is characterised in that：It is described
Box body (1) bottom is provided with backing plate (4), is connected at the top of the box body (1) by hinge with lid (2), on front side of the lid (2)
Face is provided with lockset (5), and the box body (1) is internally provided with sponge cut-off (6), and is provided with neck on sponge cut-off (6), and
Neck is multiple, and the test bottle (3) is arranged on inside neck, and test bottle (3) outer wall is brought into close contact with neck inwall, institute
State and fluted, test bottle (3) bottom and engagement are set at the top of backing plate (4), be provided with lid (2) medial surface
Buckle, and Reagent Tube (7) is provided with buckle.
A kind of 2. genetic polymorphism detection kit according to claim 1, it is characterised in that：Box body (1) outer wall
On be provided with layer protective layer (8), and protective layer (8) includes net body (9), reinforcement (10) and antibiotic layer (11), the net
Reinforcement (10) is equipped with the grid cell of body (9), the reinforcement (10) passes through the centre position of grid cell, multiple
Reinforcement (10) be arranged in parallel along the distribution arrangement of grid cell, and the antibiotic layer (11) is coated on net body (9).
A kind of 3. genetic polymorphism detection kit according to claim 1, it is characterised in that：Antibiotic layer (11) bag
Polyurethane foam (12), alkali-resistant glass fibre screen cloth (13), carbon fiber layer (14) and phenolic coating (15) are included, it is described poly-
Alkali-resistant glass fibre screen cloth (13) is provided with urethane froth bed (12), carbon is provided with the alkali-resistant glass fibre screen cloth (13)
Fibrous layer (14), phenolic coating (15) is coated with the carbon fiber layer (14).
A kind of 4. genetic polymorphism detection kit according to claim 1, it is characterised in that：In the test bottle (3)
Portion is provided with reaction system.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710753835.8A CN107384771A (en) | 2017-08-29 | 2017-08-29 | A kind of genetic polymorphism detection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710753835.8A CN107384771A (en) | 2017-08-29 | 2017-08-29 | A kind of genetic polymorphism detection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107384771A true CN107384771A (en) | 2017-11-24 |
Family
ID=60345974
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710753835.8A Pending CN107384771A (en) | 2017-08-29 | 2017-08-29 | A kind of genetic polymorphism detection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107384771A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120197218A1 (en) * | 2006-07-12 | 2012-08-02 | Mobius Therapeutics, Inc. | Apparatus and method for reconstituting a pharmaceutical and peparing the reconstituted pharmaceutical for transient application |
| CN104611995A (en) * | 2015-01-27 | 2015-05-13 | 昆明纳太能源科技有限公司 | Air filter paper and preparation method thereof |
| CN204506048U (en) * | 2014-11-12 | 2015-07-29 | 鹰柏伦科洋涂层科技（上海）有限公司 | A kind of antimicrobial coating structure |
| CN205441255U (en) * | 2016-03-11 | 2016-08-10 | 广州市康润生物科技有限公司 | Kit |
-
2017
- 2017-08-29 CN CN201710753835.8A patent/CN107384771A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120197218A1 (en) * | 2006-07-12 | 2012-08-02 | Mobius Therapeutics, Inc. | Apparatus and method for reconstituting a pharmaceutical and peparing the reconstituted pharmaceutical for transient application |
| CN204506048U (en) * | 2014-11-12 | 2015-07-29 | 鹰柏伦科洋涂层科技（上海）有限公司 | A kind of antimicrobial coating structure |
| CN104611995A (en) * | 2015-01-27 | 2015-05-13 | 昆明纳太能源科技有限公司 | Air filter paper and preparation method thereof |
| CN205441255U (en) * | 2016-03-11 | 2016-08-10 | 广州市康润生物科技有限公司 | Kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Kodama et al. | Isolation and characterization of a sulfur-oxidizing chemolithotroph growing on crude oil under anaerobic conditions | |
| Hoshino et al. | Atribacteria from the subseafloor sedimentary biosphere disperse to the hydrosphere through submarine mud volcanoes | |
| CN202486030U (en) | Simulative corrosion tester for upper deck on oil cargo tank | |
| Orcutt et al. | On the relationship between methane production and oxidation by anaerobic methanotrophic communities from cold seeps of the Gulf of Mexico | |
| CN104597240A (en) | Biosensing method for detecting leukemia by graphene/mimetic peroxidase double-signal amplification | |
| Metcalf et al. | An anaerobic, intrachamber incubator for growth of Methanosarcina spp. on methanol-containing solid media | |
| CN107384771A (en) | A kind of genetic polymorphism detection kit | |
| CN204625604U (en) | Built-up type detects wafer | |
| Pasquet et al. | Natural hydrogen potential and basaltic alteration in the Asal–Ghoubbet rift, Republic of Djibouti. | |
| Webster et al. | Diversity and composition of methanotroph communities in caves | |
| CN103805706B (en) | A kind of quadruple fluorescence quantitative kit of rapid detection SNP site | |
| Hammer et al. | Dialysis separation of sewage sludge digestion | |
| Guo et al. | Soil fungal community characteristics vary with bamboo varieties and soil compartments | |
| CN208915848U (en) | A kind of gene detecting kit | |
| CN107299049A (en) | The oral cavity cell DNA extracts kit of baby | |
| CN102634581A (en) | Fluorescent quantitative PCR (Polymerase Chain Reaction) qualitative detection method for yak-derived fiber composition | |
| CN206359528U (en) | Blood platelet RNA real-time fluorescence PCR cancer diagnosing kits | |
| CN208980740U (en) | A kind of extraction of micro source of people DNA sample, purification kit external packing box | |
| CN205127979U (en) | Reagent cabinet | |
| CN207451729U (en) | A kind of anaerobic reaction device | |
| CN202265557U (en) | Composting reactor biogas desulphurization device | |
| CN205205134U (en) | Culture dish device for it is biological experiments | |
| Nishimura et al. | Fe (III)-dependent anaerobic methane-oxidizing activity in a deep underground borehole demonstrated by in-situ pressure groundwater incubation | |
| CN208181675U (en) | A kind of novel agent box | |
| CN206127280U (en) | Centrifugation column type genome DNA extracts kit in a small amount |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171124 |
|
| RJ01 | Rejection of invention patent application after publication |