CN107376130A - Application of the LED blue light sources with 450 490nm wavelength in liver cancer treatment - Google Patents
Application of the LED blue light sources with 450 490nm wavelength in liver cancer treatment Download PDFInfo
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- CN107376130A CN107376130A CN201710530716.6A CN201710530716A CN107376130A CN 107376130 A CN107376130 A CN 107376130A CN 201710530716 A CN201710530716 A CN 201710530716A CN 107376130 A CN107376130 A CN 107376130A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N2005/0658—Radiation therapy using light characterised by the wavelength of light used
- A61N2005/0662—Visible light
- A61N2005/0663—Coloured light
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Abstract
The invention discloses application of the LED blue light sources with 450 490nm wavelength in liver cancer treatment, belong to photomedicine field.The present invention has been experimentally confirmed the LED blue lights with 450 490nm wavelength can be by suppressing the propagation of liver cancer cells, cell migration and cell invasion and then alleviating and the purpose for the treatment of liver cancer.Clinical practice of the present invention is simple and easy, and medical treatment cost is low, while alleviates patient and treat pain, and proposition of the invention provides new technological means for the treatment of liver cancer.
Description
Technical field
The present invention relates to application of the 450-490nm wavelength LED- blue light sources in liver cancer treatment.The invention belongs to light doctor
Learn technical field.
Background technology
Liver cancer is one of common malignant tumour in China, and stomach cancer and lung cancer name are only second in the dead ranking of malignant tumour
Arrange the 3rd.The number dead because suffering from liver cancer accounts for the 40% of whole world PLC mortality number every year, is a kind of serious threat people
The disease of people's life health.Under the national and effort of numerous medical personnels, treatment level and the prognosis of hepatocellular carcinoma obtain
Greatly improve, be mainly shown as the raising of early diagnostic rate, resection rate, 5 years survival rates.Liver cancer treatment pattern also tends to simultaneously
In standardization.But because liver cancer lacks special clinical manifestation and develops very rapid, evening in having belonged to the patient of clinical discovery more
Phase, now Resection Rate is relatively low, and postoperative complications are more and serious, and quickly, this causes the overall therapeutic of liver cancer to Preventive
Effect is undesirable.Therefore further strengthen liver cancer basic and clinic studies to be necessary.
Phototherapy is a kind of new treatment method occurred in recent years, has had been reported that phototherapy can trigger a variety of physiology at present
Function and effect, inventor filter out the LED- blue lights that wavelength is 450-490nm, it is found that it has to liver cancer cell growth
Obvious inhibitory action.Further probe into and find that 450-490nm LED- blue lights have and suppress cell propagation, cell migration and thin
The characteristic of born of the same parents' invasion and attack.
Clinical practice of the present invention is convenient and easy, reduces treatment cost, can mitigate patient and treat pain, is the treatment of liver cancer
New approaches are provided.
The content of the invention
The purpose of the present invention be probe into 450-490nm wavelength LED- blue light illumination liver cancer cells cell proliferation, migration and
The influence of invasion and attack, new approaches are provided for the treatment of liver cancer.
To reach above-mentioned purpose, present invention employs following technological means：
The present invention is by cultured in vitro liver cancer cell lines HepG2 and Hep3B, using the LED- blue lights of 450-490nm wavelength
Light source irradiating cell, illumination metering are respectively 0J/cm2, 72J/cm2, 144J/cm2, 216J/cm2And 288J/cm2.In illumination 24h
Afterwards, the influence bred using Trypan Blue, cell count detection 450-490nm LED- blue lights to liver cancer cell growth；Using
Scratch experiment detects the transfer ability of cell；And the invasive ability of application Transwell Cell modeling experiment detection cells.Knot
Fruit finds that 450-490nm wavelength LED- blue lights of the invention can effectively suppress hepatoma cell proliferation, migration and invasion and attack.
Therefore, the LED- blue light sources with 450-490nm wavelength are proposed and are being made based on the studies above basis, the present invention
Application in the standby apparatus for suppressing hepatoma cell proliferation.And
Application of the LED- blue light sources with 450-490nm wavelength in the apparatus for suppressing fucosylation is prepared.
And
Application of the LED- blue light sources with 450-490nm wavelength in the apparatus for suppressing liver cancer cells invasion and attack is prepared.
Relative to prior art, the beneficial effects of the invention are as follows：
By the LED- blue light illuminations with 450-490nm wavelength just can reach suppress hepatoma cell proliferation, migration with
And the purpose of invasion and attack, and then effectively alleviate and treat the purpose of liver cancer.Compared to traditional therapy, have simple easy
OK, medical treatment cost is low, mitigates the advantages that patient's treatment is painful.
Brief description of the drawings
Fig. 1 is influence of the 450-490nm LED- blue lights illumination to HepG2 liver cancer cell growths；
In HepG2 liver cancer cells, illumination 0J/cm is distinguished using LED- blue lights2, 72J/cm2, 144J/cm2, 216J/cm2
And 288J/cm2, the 24h after illumination, carry out Trypan Blue, cell count；(A) taken pictures under microscope；(B) total cell number；
(C) dead cell number；(D) percentage of dead cells；
Fig. 2 is influence of the 450-490nm LED- blue lights illumination to Hep3B liver cancer cell growths；
In Hep3B liver cancer cells, illumination 0J/cm is distinguished using LED- blue lights2, 72J/cm2, 144J/cm2, 216J/cm2
And 288J/cm2, the 24h after illumination, carry out Trypan Blue, cell count；(A) taken pictures under microscope；(B) total cell number；
(C) dead cell number；(D) percentage of dead cells；
Fig. 3 is influence of the 450-490nm LED- blue lights illumination to HepG2 fucosylations；
Using scratch experiment, LED- blue light illumination HepG2 liver cancer cells are divided into two groups：Control group illumination 0J/cm2And LED
Blue light illumination 144J/cm2The transfer ability of group, the respectively 24h after illumination, 48h and 72h Microscopic observations and detection cell of taking pictures,
(A) taken pictures under microscope；(B) statistical chart of cell migration distance；
Fig. 4 is influence of the 450-490nm LED- blue lights illumination to Hep3B fucosylations；
Using scratch experiment, LED- blue light illumination Hep3B liver cancer cells are divided into two groups：Control group illumination 0J/cm2And LED
Blue light illumination 144J/cm2The transfer ability of group, the respectively 24h after illumination, 48h and 72h Microscopic observations and detection cell of taking pictures,
(A) taken pictures under microscope；(B) statistical chart of cell migration distance；
Fig. 5 is the influence that 450-490nm LED- blue lights illumination is attacked to HepG2 and Hep3B liver cancer cells.
Tested using Transwell Cell modelings, LED- blue light illumination HepG2 and Hep3B liver cancer cells are divided into two groups：It is right
According to a group illumination 0J/cm2With LED blue light illumination 144J/cm2Group, the invasion and attack of 24h Microscopic observations and detection cell of taking pictures after illumination
Ability.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art
Member to the details and form of technical solution of the present invention it should be understood that can enter without departing from the spirit and scope of the invention
Row modifications or substitutions, but these modifications and replacement are each fallen within protection scope of the present invention.
Material and its source involved by the embodiment of the present invention：
1. main agents：Trypan blue reagent (biosharp companies)
2. key instrument：Count Star cell counters；Inverted microscope
Embodiment 1：The illumination of 450-490nm LED- blue lights suppresses liver cancer cell growth
1 experimental method
1.1 experiment packets design：
Experiment is divided into following five groups according to 450-490nm LED- blue light intensities of illumination:0J/cm2, 72J/cm2, 144J/
cm2, 216J/cm2And 288J/cm2.The HepG2 liver cancer cells and Hep3B liver cancer cells of equivalent cell numbers amount are irradiated respectively.
1.2 Trypan Blues detect liver cancer cells survival rate
The 24h after illumination, carry out Trypan Blue, cell count.
1.3 data processing
The result of the present invention is represented using standard deviation ± standard error.Mapped with Graphpad prism 5.0, respectively
Correlation between group is weighed with T inspections.
2 observation results
2.1 450-490nm LED- blue lights illumination suppress HepG2 cell growths
Trypan Blue, cell counts are as shown in figure 1, and 0J/cm2Light group ratio, cell under light group cell mirror
Form is substantially rounded and brightened, and dead cell number and dead cell rate dramatically increase, and viable count significantly reduces.
2.2 450-490nm LED- blue lights illumination suppress Hep3B liver cancer cell growths
Trypan Blue, cell counts are as shown in Fig. 2 and 0J/cm2Light group ratio, light group viable count are obvious
Reduce, dead cell number and cell rate are significantly raised, and act on enhancing with time lengthening.
The above results illustrate as the enhancing of 450-490nm wavelength LED- blue light intensities of illumination, liver cancer viable count are obvious
Reduce, dead cell number and dead cell rate are significantly raised.Illustrate that 450-490nm wavelength LED- blue lights illumination can suppress liver cancer cells
Propagation.
Embodiment 2：The illumination of 450-490nm LED- blue lights suppresses fucosylation
1 experimental method
1.1 experiment packets design：
Experiment is divided into 2 groups：450-490nm LED- blue light illumination 0J/cm2Group and 450-490nm LED- blue light illumination
144J/cm2The HepG2 liver cancer cells and Hep3B liver cancer cells of group, respectively irradiation equivalent cell numbers amount, the 24h after illumination,
48h and 72h Microscopic observations and the migration situation for photographing to record cell.
1.2 scratch experiments detect fucosylation ability
Cut is carried out in HepG2 liver cancer cells and Hep3B liver cancer cells surface using 200 μ l pipette tips, makes scratch width
It is consistent as far as possible, the micro- Microscopic observation of the 24h after illumination, 48h and 72h and photographs to record cell migration situation respectively.
1.3 data processing
The result of the present invention is represented using standard deviation ± standard error.Mapped with Graphpad prism 5.0, respectively
Correlation between group is weighed with T inspections.
2 observation results
2.1 450-490nm LED- blue lights illumination suppress HepG2 cell migrations
Scratch experiment result as shown in figure 3, with control group ratio, LED- blue light illumination 144J/cm2Cell moves group after 24h
It is obvious suppressed to move distance, elapses over time, the inhibitory action enhancing of cell migration ability after illumination 48h and 72h.
2.2 450-490nm LED- blue lights illumination suppress Hep3B cell migrations
Scratch experiment result as shown in figure 4, with control group ratio, LED- blue light illumination 144J/cm2Cell moves group after 24h
It is obvious suppressed to move distance, elapses over time, the inhibitory action enhancing of cell migration ability after illumination 48h and 72h.
It these results suggest that 450-490nm wavelength LED- blue lights illumination can suppress fucosylation.
Embodiment 3：The illumination of 450-490nm LED- blue lights suppresses liver cancer cells invasion and attack
1 experimental method
(1.1) Transwell Cell modelings experiment detection Invasive Ability of Hepatocellular Carcinoma
1.2 experiment packets design：
Experiment is divided into 2 groups：450-490nm LED- blue light illumination 0J/cm2Control group and illumination 144J/cm2Light group,
24h Microscopic observations and the invasion and attack situation of cell is photographed to record after illumination.
1.3 experiment detection projects：Transwell Cell modelings are tested
Tested using Transwell Cell modelings, in two kinds of liver cancer cells of HepG2 and Hep3B, be inoculated with cell upper strata
Liver cancer cells, the cell number of observation invasion and attack to cell lower floor, experiment are divided into two groups of 0J/cm2Light control group and LED- blue light light
According to 144J/cm2Light group.The 24h after illumination respectively, micro- Microscopic observation simultaneously photograph to record cell invasion situation.
1.4 data processing
The result of the present invention is represented using standard deviation ± standard error.Mapped with Graphpad prism 5.0, respectively
Correlation between group is weighed with T inspections.
2 observation results
Transwell Cell modelings experimental result as shown in figure 5, and contrast ratio, light group cell lower floor after illumination 24h
Cell number significantly reduces, and illustrates that the invasive ability of liver cancer cells is substantially suppressed.Illustrate 450-490nm wavelength LED- blue light light
According to the invasion and attack for suppressing liver cancer cells.
Claims (3)
1. application of the LED- blue light sources with 450-490nm wavelength in the apparatus for suppressing hepatoma cell proliferation is prepared.
2. application of the LED- blue light sources with 450-490nm wavelength in the apparatus for suppressing fucosylation is prepared.
3. application of the LED- blue light sources with 450-490nm wavelength in the apparatus for suppressing liver cancer cells invasion and attack is prepared.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710530716.6A CN107376130A (en) | 2017-07-03 | 2017-07-03 | Application of the LED blue light sources with 450 490nm wavelength in liver cancer treatment |
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| CN201710530716.6A CN107376130A (en) | 2017-07-03 | 2017-07-03 | Application of the LED blue light sources with 450 490nm wavelength in liver cancer treatment |
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| CN107376130A true CN107376130A (en) | 2017-11-24 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4359103B2 (en) * | 2003-08-22 | 2009-11-04 | 合同会社希少糖生産技術研究所 | Cancer cell growth suppression apparatus provided with LED irradiation means in the presence of rare sugar |
| CN105870304A (en) * | 2016-04-22 | 2016-08-17 | 江苏脉锐光电科技有限公司 | LED light source with adjustable wavelength |
-
2017
- 2017-07-03 CN CN201710530716.6A patent/CN107376130A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4359103B2 (en) * | 2003-08-22 | 2009-11-04 | 合同会社希少糖生産技術研究所 | Cancer cell growth suppression apparatus provided with LED irradiation means in the presence of rare sugar |
| CN105870304A (en) * | 2016-04-22 | 2016-08-17 | 江苏脉锐光电科技有限公司 | LED light source with adjustable wavelength |
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Application publication date: 20171124 |