CN107362458A - Application of the LED blue light sources with 450 490 nm wavelength in treatment of colon cancer - Google Patents
Application of the LED blue light sources with 450 490 nm wavelength in treatment of colon cancer Download PDFInfo
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- CN107362458A CN107362458A CN201710530761.1A CN201710530761A CN107362458A CN 107362458 A CN107362458 A CN 107362458A CN 201710530761 A CN201710530761 A CN 201710530761A CN 107362458 A CN107362458 A CN 107362458A
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- colon cancer
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- blue light
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N2005/065—Light sources therefor
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
- A61N5/00—Radiation therapy
- A61N5/06—Radiation therapy using light
- A61N2005/0658—Radiation therapy using light characterised by the wavelength of light used
- A61N2005/0662—Visible light
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- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Radiology & Medical Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
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- Veterinary Medicine (AREA)
- Radiation-Therapy Devices (AREA)
Abstract
The invention discloses application of the LED blue light sources with 450 490nm wavelength in treatment of colon cancer, belong to photomedicine field.The present invention has been experimentally confirmed the LED blue lights with 450 490nm wavelength can be by suppressing Colon Cancer Cells and migration and then the purpose for alleviating and treating colon cancer.Clinical practice of the present invention is simple and easy, and medical treatment cost is low, while alleviates patient and treat pain, and proposition of the invention provides new technological means for the treatment of colon cancer.
Description
Technical field
The present invention relates to application of the 450-490nm wavelength LED- blue light sources in treatment of colon cancer.The invention belongs to light
Medicine technology field.
Background technology
Colon cancer belongs to malignant tumor of digestive tract, is one of common cancer for seriously endangering human health, and fall ill
Rate is in the trend risen year by year, and most of patients is found to have belonged to middle and advanced stage.Colorectal cancer clinical treatment at present is mainly with surgery excision
Based on tumour, while purposefully it is aided with chemicotherapy, targeted therapy, gene therapy etc..With the raising of medical level, colon cancer
The development of therapy field make rapid progress.But existing treatment means still suffer from the defects of certain, as operation cause it is irreversible
Wound.Therefore, it is badly in need of a kind of safely and effectively treatment means, is protecting patient from treating colon cancer while wound.
Light therapy is a kind of new treatment method occurred in recent years, and currently reported phototherapy can adjust a variety of physiology
Function and effect, we filter out the LED- blue lights that wavelength is 450-490nm, it is found that it has significantly to Growth of Colon Cancer Cells
Inhibitory action.Further probe into and find that 450-490nm LED- blue lights have the characteristic for suppressing Colon Cancer Cells and migration.
Clinical practice of the present invention is simple and easy, reduces medical treatment cost, mitigates patient and treats pain, is carried for the treatment of colon cancer
New approaches are supplied.
The content of the invention
The purpose of the present invention is to probe into the LED- blue lights illumination of 450-490nm wavelength to Colon Cancer Cells and the shadow of migration
Ring, new approaches are provided for the treatment of colon cancer.
To reach above-mentioned purpose, present invention employs following technological means：
The present invention is by cultured in vitro colon carcinoma cell line SW620 and HT29, using the LED- blue lights of 450-490nm wavelength
Light source irradiating cell, intensity of illumination are respectively 0J/cm2, 72J/cm2, 144J/cm2, 216J/cm2And 288J/cm2.In illumination 24h
Afterwards, the influence bred using Trypan Blue, cell count detection 450-490nm LED- blue lights to Growth of Colon Cancer Cells；Should
With the transfer ability of scratch experiment detection cell.As a result prove, 450-490nm wavelength LED- blue lights of the invention can effectively press down
Colon Cancer Cells processed and migration.
Therefore, the LED- blue light sources with 450-490nm wavelength are proposed and are being made based on the studies above basis, the present invention
Application in standby treatment colon cancer apparatus.
Wherein, the blue light for the 450-490nm wavelength that described LED- blue light sources are sent is by suppressing colon cancer cell
Propagation and migration, reach the purpose for effectively alleviating and treating colon cancer.
Relative to prior art, the beneficial effects of the invention are as follows：
It can just be reached by the LED- blue light illuminations with 450-490nm wavelength and suppress Colon Cancer Cells and move
Move, and then effectively alleviate and treat the purpose of colon cancer.Compared to traditional therapy, have it is simple and easy, medical treatment into
This is low, mitigates the advantages that patient's treatment is painful.
Brief description of the drawings
Fig. 1 is influence of the 450-490nm LED- blue lights illumination to SW620 Growth of Colon Cancer Cells；
It is 0J/cm using intensity of illumination2, 72J/cm2, 144J/cm2, 216J/cm2And 288J/cm2LED- blue lights difference
Illumination SW620 colon cancer cells；The 24h after illumination, carry out Trypan Blue, cell count；(A) taken pictures under microscope；(B) it is living
TCS；(C) dead cell sum；(D) percentage of dead cells；
Fig. 2 is influence of the 450-490nm LED- blue lights illumination to HT29 Growth of Colon Cancer Cells；
It is 0J/cm using intensity of illumination2, 72J/cm2, 144J/cm2, 216J/cm2And 288J/cm2LED- blue lights difference
Illumination HT29 colon cancer cells；24h after illumination, carry out Trypan Blue and cell count；(A) taken pictures under microscope；(B) it is living thin
Born of the same parents' sum；(C) dead cell sum；(D) percentage of dead cells；
Fig. 3 is the influence that 450-490nm LED- blue lights illumination migrates to SW620 colon cancer cells；
Using scratch experiment, LED- blue light illumination SW620 colon cancer cells are divided into two groups of illumination 0J/cm2Control group and LED
Blue light illumination 144J/cm2Light group；The migration energy of the 24h after illumination respectively, 48h and 72h Microscopic observation and detection cell of taking pictures
Power；(A) taken pictures under microscope；(B) statistical chart of cell migration distance；
Fig. 4 is the influence that 450-490nm LED- blue lights illumination migrates to HT29 colon cancer cells；
Using scratch experiment, LED- blue light illumination HT29 colon cancer cells are divided into two groups：Control group illumination 0J/cm2And LED
Blue light illumination 144J/cm2Group；The transfer ability of the 24h after illumination respectively, 48h and 72h Microscopic observation and detection cell of taking pictures；
(A) taken pictures under microscope；(B) statistical chart of cell migration distance.
Embodiment
The invention will now be further described with reference to specific embodiments, advantages of the present invention and feature will be with description and
It is apparent.But these embodiments are only exemplary, do not form any restrictions to the scope of the present invention.People in the art
Member to the details and form of technical solution of the present invention it should be understood that can enter without departing from the spirit and scope of the invention
Row modifications or substitutions, but these modifications and replacement are each fallen within protection scope of the present invention.
Material and its source involved by the embodiment of the present invention：
1. main agents：Trypan blue reagent (biosharp companies)
2. key instrument：Count Star cell counters；Fluorescence inverted microscope；Inverted microscope
Embodiment 1：The illumination of 450-490nm LED- blue lights suppresses Growth of Colon Cancer Cells
1 experimental method
1.1 experiment packets design：
Experiment is divided into following five groups according to 450-490nm LED- blue light intensities of illumination:0J/cm2, 72J/cm2, 144J/
cm2, 216J/cm2And 288J/cm2.The SW620 colon cancer cells and HT29 colon cancer cells of identical quantity are irradiated respectively.
1.2 Trypan Blues detect colon cancer cell survival rate
The 24h after illumination, carry out Trypan Blue, cell count.
1.3. data processing
The result of the present invention is represented using standard deviation ± standard error.Mapped with Graphpad prism 5.0, respectively
Correlation between group is weighed with T inspections.
2 observation results
2.1 450-490nm LED- blue lights illumination suppress SW620 cell growths
Trypan Blue, cell counts are as shown in figure 1, and 0J/cm2Light group ratio, illumination 72J/cm2Group is without obvious
Change, illumination 144J/cm2, 216J/cm2And 288J/cm2Cellular morphology is substantially rounded and brightened under group mirror, and dead cell number and
Dead cell rate dramatically increases, and viable count significantly reduces.
2.2 450-490nm LED- blue lights illumination suppress HT29 cell growths
Trypan Blue, cell counts are as shown in Fig. 2 and 0J/cm2Light group ratio, HT29 colon cancer cell illumination
72J/cm2Group is without significant change, illumination 144J/cm2Group viable count substantially reduces, illumination 216J/cm2And 288J/cm2Group is dead
Cell number and dead cell rate rise rapidly, are 288J/cm in light energy2Group, dead cell rate reach more than 60%.
The above results illustrate as the enhancing of 450-490nm wavelength LED- blue light intensities of illumination, colon cancer viable count are bright
Aobvious to reduce, dead cell number and dead cell rate are significantly raised.Illustrate that 450-490nm wavelength LED- blue lights illumination can suppress colon cancer
Cell is bred.
Embodiment 2：The illumination of 450-490nm LED- blue lights suppresses colon cancer cell migration
1 experimental method
1.1 experiment packets design：
Experiment is divided into 2 groups：450-490nm LED- blue light illumination 0J/cm2Group and 450-490nm LED- blue light illumination
144J/cm2Group, irradiates the SW620 colon cancer cells and HT29 colon cancer cells of identical quantity respectively, the 24h after illumination,
48h and 72h Microscopic observations and the migration situation for photographing to record cell.
1.2 scratch experiments detect colon cancer cell transfer ability
Cut is carried out in SW620 colon cancer cells and HT29 colon cancer cells surface using 200 μ l pipette tips, makes cut wide
Degree is consistent as far as possible, the micro- Microscopic observation of the 24h after illumination, 48h and 72h and photographs to record cell migration situation respectively.
1.3. data processing
The result of the present invention is represented using standard deviation ± standard error.Mapped with Graphpad prism 5.0, respectively
Correlation between group is weighed with T inspections.
2 observation results
2.1 450-490nm LED- blue lights illumination suppress SW620 cell migrations
Scratch experiment result as shown in figure 3, with control group ratio, LED- blue light illumination 144J/cm2Cell moves group after 24h
Distance is moved without significant change, is elapsed over time, cell migration ability is substantially suppressed after illumination 48h and 72h.
2.2 450-490nm LED- blue lights illumination suppress HT29 cell migrations
Scratch experiment result as shown in figure 4, with control group ratio, colon cancer cell LED- blue light illumination 144J/cm2Group cell
Transfer ability is substantially suppressed, and the inhibitory action enhancing migrated as time went on.
It these results suggest that 450-490nm wavelength LED- blue lights illumination can suppress colon cancer cell migration.
Claims (2)
1. the LED- blue light sources with 450-490nm wavelength are preparing the application in treating colon cancer apparatus.
2. application as claimed in claim 1, it is characterised in that the 450-490nm ripples that described LED- blue light sources are sent
Long blue light reaches the purpose for effectively alleviating and treating colon cancer by suppressing Colon Cancer Cells and migration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710530761.1A CN107362458A (en) | 2017-07-03 | 2017-07-03 | Application of the LED blue light sources with 450 490 nm wavelength in treatment of colon cancer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710530761.1A CN107362458A (en) | 2017-07-03 | 2017-07-03 | Application of the LED blue light sources with 450 490 nm wavelength in treatment of colon cancer |
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| Publication Number | Publication Date |
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| CN107362458A true CN107362458A (en) | 2017-11-21 |
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| CN201710530761.1A Pending CN107362458A (en) | 2017-07-03 | 2017-07-03 | Application of the LED blue light sources with 450 490 nm wavelength in treatment of colon cancer |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005065977A (en) * | 2003-08-22 | 2005-03-17 | Kagawa Univ | Method for suppressing cancer cell with led and rare sugar |
| CN106237533A (en) * | 2016-08-24 | 2016-12-21 | 中国医学科学院生物医学工程研究所 | The miniature human body inner chamber photodynamic therapy system that a kind of noinvasive is controlled |
-
2017
- 2017-07-03 CN CN201710530761.1A patent/CN107362458A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005065977A (en) * | 2003-08-22 | 2005-03-17 | Kagawa Univ | Method for suppressing cancer cell with led and rare sugar |
| CN106237533A (en) * | 2016-08-24 | 2016-12-21 | 中国医学科学院生物医学工程研究所 | The miniature human body inner chamber photodynamic therapy system that a kind of noinvasive is controlled |
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Application publication date: 20171121 |