CN107372889A - A kind of Ramulus et Folium Mussaendae Pubescentis extract manufacture craft with high inhibition MMPs vigor - Google Patents
A kind of Ramulus et Folium Mussaendae Pubescentis extract manufacture craft with high inhibition MMPs vigor Download PDFInfo
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- CN107372889A CN107372889A CN201710738474.XA CN201710738474A CN107372889A CN 107372889 A CN107372889 A CN 107372889A CN 201710738474 A CN201710738474 A CN 201710738474A CN 107372889 A CN107372889 A CN 107372889A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/06—Treating tea before extraction; Preparations produced thereby
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/166—Addition of, or treatment with, enzymes or microorganisms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/22—Drying or concentrating tea extract
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
- A23F3/22—Drying or concentrating tea extract
- A23F3/26—Drying or concentrating tea extract by lyophilisation
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
- A23F3/00—Tea; Tea substitutes; Preparations thereof
- A23F3/40—Tea flavour; Tea oil; Flavouring of tea or tea extract
- A23F3/405—Flavouring with flavours other than natural tea flavour or tea oil
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
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- Medicines Containing Plant Substances (AREA)
- Cosmetics (AREA)
Abstract
White tea is one of traditional six big teas, and its preparation method is unique, does not fry and does not rub, and full pekoe is draped over one's shoulders into tea appearance, white is presented, therefore claim " white tea ".White tea originate in the Fuding in Fujian, have stable political situation, Jianyang, the ground such as Songxi, be one of distinctive teas in Fujian.Modern study shows that Ramulus et Folium Mussaendae Pubescentis extract can suppress MMPs activity, compare green tea, though it integrally suppresses MMPs activity by force, but gap and unobvious, the possible factor for causing this result is that catechin content is low compared with green tea in white tea.The present invention generates gene by UV-activated catechin, and can reach 180~260mg/g through corresponding white tea manufacture craft and extraction process, the content of extract obtained middle catechin, high more than 6 times compared with white tea obtained by traditional handicraft, and lifting effect is fairly obvious.
Description
Technical field
The present invention relates to a kind of Ramulus et Folium Mussaendae Pubescentis extract manufacture craft with high inhibition MMPs vigor.
Background technology
White tea is one of traditional six big teas, and its preparation method is unique, does not fry and does not rub, drapes over one's shoulders full pekoe into tea appearance, white is presented,
Therefore claim " white tea ".White tea originate in the Fuding in Fujian, have stable political situation, Jianyang, the ground such as Songxi, be one of distinctive teas in Fujian.
Matrix metalloproteinase MMPs is one group of Zn2+ dependence endopeptidase, has now been found that 26 kinds, is distributed widely in plant
In thing, vertebrate, invertebrate, all the components for the ECM that can almost be degraded in extracellular matrix (ECM) degradation process.
MMPs is divided into five major classes according to the difference of substrate specificity and structure：Clostridiopetidase A, gelatinase, stromlysin, membranous type MMP, other
Type MMP.Found in current skin aging research, collagen and elastin laminin in the degradable corium of MMPs, are light
Outside aging and free radical aging, cause one of most important factor of skin senescence.
Modern study shows that tea extract has the suppression of the obvious ability, wherein Ramulus et Folium Mussaendae Pubescentis extract for suppressing MMPs activity
Ability processed is better than green-tea extract, this effect be white tea by inference because processing link is less remain more have it is anti-oxidant
The result of active material.But in these materials, the catechin content of white tea will be significantly lower than green tea, and its catechin content is usually
30mg/g or so, and the catechin content of green tea can reach more than 100mg/g.
Recently, Tea Inst., Chinese Academy of Agricultural Sciences's Tea Germplasm innovation team researcher is based on different heredity
Colony's (natural population and hybridization segregating population) of background, integrated use hybrid separation transcript profile sequencing (BSR-Seq), Hou Xuanji
Because of a variety of research meanses such as association analysis, a critical function gene for influenceing catechin synthesis is identified in tea tree ---
The-Kongization enzyme of flavonoids 3 ', 5 ' (H of F3 ' 5 ') gene.Research finds there is 10 function SNP and catechin in the H gene sites of F3 ' 5 '
The characters such as index CI (i.e. dihydroxy catechin/trihydroxy catechin), catechin composition content and catechin total amount are significantly correlated.
The content of the invention
The purpose of the application patent, it is that the inducing action of gene is lifted in the manufacturing process of white tea using ultraviolet
The content of its catechin, and by the leaching efficiency of active principle in biotechnology raising tealeaves, obtain with high inhibition MMPs
The Ramulus et Folium Mussaendae Pubescentis extract of vigor.
Specific method：
1. harvesting
Under certain temperature condition, pluck fresh and tender leaf, stay handle short, and gently adopt and put down gently, be placed in container made of bamboo contain and
Storing.Due to being finally to produce extract, therefore the size to fresh leaf, the uniformity, damaged it is not strict with；
2. ultraviolet irradiation and air-dry wither
Blade after harvesting is uniformly dissipated bottom is not with holes and in bamboo cylindrical storage container that surrounding is with holes, circle
The blade diameter length ratio of cylindricality is 10~20: 1.In storage container, blade is unsuitable overlapping, the top placement uviol lamp of container, storage container
Outer placement fan.During withering, fan is opened, the blast velocity control above blade is in 1~5m/min, and temperature control is 20~30
DEG C, relative humidity is controlled 65~80%.Wither beginning when, open uviol lamp, there is provided 300~600nm ultraviolet irradiation, with
Open 1~2h of frequency prolonged exposure that 10min closes 10min.To 6 seventy percent it is dry when, blade does not paste sieve, bud-leaf milli color turns white, leaf color by
It is light green switch to dark green or grayish green, bud it is sharp stick up with tender stalk tail, leaf margin slightly hang down volume, blade face have corrugated, smell without green gas when, you can
Carry out and sieve, 2~4cm of heap thickness in sieve；To seventy percent it is dry when, continue and sieve, 4~7cm of heap thickness in sieve；To eighty per cant it is dry when again simultaneously
Sieve, 5~10cm of heap thickness in sieve；Be placed on the frame that withers and continue to wither 10 hours, reach ninety percent it is dry when, it is lower sieve drying.
3. dry simultaneously lysed cells structure
1~5% folium cortex eucommiae is incorporated in ninety percent dry tealeaves, is dried using air flow dryer, mixing folium cortex eucommiae can carry
For the fragrance of uniqueness.First using 80~90 DEG C of drying 10min, then spread placement out, when temperature is cooled to room temperature, into leaf uniformly
The cellulase solution configured in advance is sprayed, its cellulase amount is the 1~2% of leaf weight, and water is the 2~4% of leaf weight, is then made
4~5h is dried again with 40~50 DEG C.After the completion of drying, the catechin content in white tea can reach 80~120mg/g.
It is 4. broken
Using machinery is pulverized, the tealeaves after drying is pulverized into 20~40 mesh.
5. extraction
By cellulase and mechanical crushing to cyto-architectural destruction, low temperature extraction can be achieved.10~15 are weighed using leaf
Water again, under vacuum conditions, normal temperature circulation extract 2~5h.Centrifuged leaching liquor after the completion of extraction.
6. concentration
Leaching liquor is concentrated using NF membrane of the molecular cut off in 100~500Dal, is concentrated into containing for solid content
Amount reaches 15~25% cut-offs.
7. dry
The vitamin C of solid content 1~1.5% is accounted in the concentrate addition of NF membrane, freeze-drying is used after being well mixed
The final Ramulus et Folium Mussaendae Pubescentis extract with high inhibition MMPs vigor is obtained, the content of wherein catechin can reach 180~260mg/g.
Embodiment
Case 1：
1) is plucked
Under certain temperature condition, pluck fresh and tender leaf, stay handle short, and gently adopt and put down gently, be placed in container made of bamboo contain and
Storing.Due to being finally to produce extract, therefore the size to fresh leaf, the uniformity, damaged it is not strict with；
2) ultraviolet irradiations and air-dry wither
Take the blade 5kg after harvesting, uniformly dissipate bottom is not with holes and bamboo cylindrical storage container that surrounding is with holes
In, cylindrical blade diameter length ratio is 10: 1.In storage container, blade is unsuitable overlapping.The top placement uviol lamp of container, storage container
Outer placement fan.During withering, fan is opened, the blast velocity control above blade is in 1.5m/min, and temperature control is at 20 DEG C, phase
To humid control 80%.Wither beginning when, open uviol lamp, there is provided 380nm ultraviolet irradiation, with open 10min close 10min
Frequency prolonged exposure 1h.To 6 seventy percent it is dry when, blade does not paste sieve, bud-leaf milli color turns white, leaf color by it is light green switch to it is dark green or grey
Green, bud is sharp stick up with tender stalk tail, leaf margin slightly hang down volume, blade face have corrugated, smell without green gas when, you can carry out and sieve, in sieve
2~4cm of heap thickness；To seventy percent it is dry when, continue and sieve, 4~7cm of heap thickness in sieve；To eighty per cant it is dry when again and sieve, the heap thickness 5 in sieve
~10cm；Be placed on the frame that withers and continue to wither 10 hours, reach ninety percent it is dry when, it is lower sieve drying.
3) drying and lysed cells structure
1% folium cortex eucommiae is incorporated in ninety percent dry tealeaves, is dried using air flow dryer, mixing folium cortex eucommiae can provide solely
Special fragrance.First using 90 DEG C of drying 10min, then spread placement out, when temperature is cooled to room temperature, uniformly sprinkling shifts to an earlier date into leaf
The cellulase solution of configuration, its cellulase amount are the 1% of leaf weight, and water is the 2% of leaf weight, then dries 5h again using 45 DEG C.
After the completion of drying, the catechin content in white tea can reach 83.3mg/g.
4) is crushed
Using machinery is pulverized, the tealeaves after drying is pulverized into 20 mesh.
5) is extracted
By cellulase and mechanical crushing to cyto-architectural destruction, low temperature extraction can be achieved.10 times are weighed using leaf
Water, under vacuum conditions, normal temperature circulation extraction 3h.Centrifuged leaching liquor after the completion of extraction.
6) is concentrated
Leaching liquor is concentrated using NF membrane of the molecular cut off in 180Dal, the content for being concentrated into solid content reaches
17% cut-off.
7) is dried
The vitamin C of solid content 1% is accounted in the concentrate addition of NF membrane, is obtained most using freeze-drying after well mixed
The whole Ramulus et Folium Mussaendae Pubescentis extract with high inhibition MMPs vigor.The content of wherein catechin can reach 194mg/g.
Case 2：
1) is plucked
Under certain temperature condition, pluck fresh and tender leaf, stay handle short, and gently adopt and put down gently, be placed in container made of bamboo contain and
Storing.Due to being finally to produce extract, therefore the size to fresh leaf, the uniformity, damaged it is not strict with；
2) ultraviolet irradiations and air-dry wither
Take the blade 10kg after harvesting, uniformly dissipate bottom is not with holes and bamboo cylindrical storage container that surrounding is with holes
In, cylindrical blade diameter length ratio is 18: 1.In storage container, blade is unsuitable overlapping.The top placement uviol lamp of container, storage container
Outer placement fan.During withering, fan is opened, the blast velocity control above blade is in 3.5m/min, and temperature control is at 25 DEG C, phase
To humid control 65%.Wither beginning when, open uviol lamp, there is provided 380nm ultraviolet irradiation, with open 10min close 10min
Frequency prolonged exposure 1.5h.To 6 seventy percent it is dry when, blade does not paste sieve, bud-leaf milli color turns white, leaf color by it is light green switch to it is dark green or
Grayish green, bud is sharp stick up with tender stalk tail, leaf margin slightly hang down volume, blade face have corrugated, smell without green gas when, you can carry out and sieve, sieving
2~4cm of middle heap thickness；To seventy percent it is dry when, continue and sieve, 4~7cm of heap thickness in sieve；To eighty per cant it is dry when again and sieve, heap is thick in sieve
5~10cm；Be placed on the frame that withers and continue to wither 10 hours, reach ninety percent it is dry when, it is lower sieve drying.
3) drying and lysed cells structure
1.5% folium cortex eucommiae is incorporated in ninety percent dry tealeaves, is dried using air flow dryer, mixing folium cortex eucommiae can provide
Unique fragrance.First using 80 DEG C of drying 10min, then spread placement out, when temperature is cooled to room temperature, uniformly sprinkling carries into leaf
The cellulase solution of preceding configuration, its cellulase amount are the 2% of leaf weight, and water is the 4% of leaf weight, then using 40 DEG C of multiple bakings
4h.After the completion of drying, the catechin content in white tea can reach 117.4mg/g.
4) is crushed
Using machinery is pulverized, the tealeaves after drying is pulverized into 40 mesh.
5) is extracted
By cellulase and mechanical crushing to cyto-architectural destruction, low temperature extraction can be achieved.15 times are weighed using leaf
Water, under vacuum conditions, normal temperature circulation extraction 5h.Centrifuged leaching liquor after the completion of extraction.
6) is concentrated
Leaching liquor is concentrated using NF membrane of the molecular cut off in 300Dal, the content for being concentrated into solid content reaches
20% cut-off.
7) is dried
The vitamin C of solid content 1.5% is accounted in the concentrate addition of NF membrane, is obtained after well mixed using freeze-drying
The final Ramulus et Folium Mussaendae Pubescentis extract with high inhibition MMPs vigor.The content of wherein catechin can reach 251mg/g.
Embodiments of the invention are the foregoing is only, are not intended to limit the scope of the invention, it is every to utilize this hair
The equivalent transformation that bright description is made, or other related technical areas are directly or indirectly used in, similarly it is included in
In the scope of patent protection of the present invention.
Claims (3)
1. a kind of Ramulus et Folium Mussaendae Pubescentis extract manufacture craft with high inhibition MMPs vigor, comprises the following steps：
1) pluck
Under certain temperature condition, fresh and tender leaf is plucked, stays handle short, and gently adopts and puts down gently, container made of bamboo is placed in and contains and store and transport.
Due to being finally to produce extract, therefore the size to fresh leaf, the uniformity, damaged it is not strict with；
2) ultraviolet irradiation and air-dry wither
Blade after harvesting is uniformly dissipated bottom is not with holes and in bamboo cylindrical storage container that surrounding is with holes, cylinder
Blade diameter length ratio be 10~20: 1.In storage container, blade is unsuitable overlapping.The top placement uviol lamp of container, storage container are pacified outside
Put fan.During withering, open fan, the blast velocity control above blade in 1~5m/min, temperature control at 20~30 DEG C,
Relative humidity is controlled 65~80%.Wither beginning when, open uviol lamp, there is provided 300~600nm ultraviolet irradiation, to open
10min closes 10min 1~2h of frequency prolonged exposure.To 6 seventy percent it is dry when, blade does not paste sieve, bud-leaf milli color turns white, leaf color is by shallow
It is green switch to dark green or grayish green, bud it is sharp stick up with tender stalk tail, leaf margin slightly hang down volume, blade face have corrugated, smell without green gas when, you can enter
Go and sieve, 2~4cm of heap thickness in sieve；To seventy percent it is dry when, continue and sieve, 4~7cm of heap thickness in sieve；To eighty per cant it is dry when again simultaneously
Sieve, 5~10cm of heap thickness in sieve；Be placed on the frame that withers and continue to wither 10 hours, reach ninety percent it is dry when, it is lower sieve drying.
3) drying and lysed cells structure
1~5% folium cortex eucommiae is incorporated in ninety percent dry tealeaves, is dried using air flow dryer, mixing folium cortex eucommiae can provide solely
Special fragrance.First using 80~90 DEG C of drying 10min, then spread placement out, when temperature is cooled to room temperature, uniformly sprayed into leaf
The cellulase solution configured in advance, its cellulase amount are the 1~2% of leaf weight, and water is the 2~4% of leaf weight, then using 40
~50 DEG C are dried 4~5h again.After the completion of drying, the catechin content in white tea can reach 80~120mg/g.
4) crush
Using machinery is pulverized, the tealeaves after drying is pulverized into 20~40 mesh.
5) extract
By cellulase and mechanical crushing to cyto-architectural destruction, low temperature extraction can be achieved.10~15 times are weighed using leaf
Water, under vacuum conditions, normal temperature circulation extract 2~5h.Centrifuged leaching liquor after the completion of extraction.
6) concentrate
Leaching liquor is concentrated using NF membrane of the molecular cut off in 100~500Da1, the content for being concentrated into solid content reaches
To 15~25% cut-offs.
7) dry
The vitamin C of solid content 1~1.5% is accounted in the concentrate addition of NF membrane, is obtained after well mixed using freeze-drying
The final Ramulus et Folium Mussaendae Pubescentis extract with high inhibition MMPs vigor, the content of wherein catechin can reach 180~260mg/g.
2. the Ramulus et Folium Mussaendae Pubescentis extract preparation method according to claim 1 with high inhibition MMPs vigor, it is characterised in that adopt
Blade after plucking is withered using ultraviolet irradiation and air-drying：Blade after harvesting is uniformly dissipated not with holes in bottom and surrounding is with holes
Bamboo cylindrical storage container in, cylindrical blade diameter length ratio be 10~20: 1.In storage container, blade is unsuitable overlapping.Container
Top placement uviol lamp, dispose fan outside storage container.During withering, fan is opened, the blast velocity control above blade is 1
~5m/min, at 20~30 DEG C, relative humidity controls 65~80% temperature control.Wither beginning when, open uviol lamp, there is provided
300~600nm ultraviolet irradiation, to open 1~2h of frequency prolonged exposure that 10min closes 10min.To 6 seventy percent it is dry when, blade
Sieve is not pasted, bud-leaf milli color turns white, leaf color is slightly hung down volume, blade face by light green sharp tail, the leaf margin of sticking up with tender stalk of dark green or grayish green, bud that switch to
Have corrugated, smell without green gas when, you can carry out and sieve, 2~4cm of heap thickness in sieve；To seventy percent it is dry when, continue and sieve, sieving
4~7cm of middle heap thickness；To eighty per cant it is dry when again and sieve, 5~10cm of heap thickness in sieve；It is placed on the frame that withers and continues to wither 10 hours, reaches
To ninety percent it is dry when, it is lower sieve drying.
3. the Ramulus et Folium Mussaendae Pubescentis extract preparation method according to claim 1 with high inhibition MMPs vigor, it is characterised in that right
Ninety percent dry blade dry and lysed cells structure：In drying course, 1~5% Du is incorporated in ninety percent dry tealeaves
Zhong Ye, dried using air flow dryer, mixing folium cortex eucommiae can provide the fragrance of uniqueness.First using 80~90 DEG C of drying 10min, then
Spread placement out, when temperature is cooled to room temperature, the cellulase solution configured in advance, its cellulase amount are uniformly sprayed into leaf
For the 1~2% of leaf weight, water is the 2~4% of leaf weight, then dries 4~5h again using 40~50 DEG C.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109007101A (en) * | 2018-09-30 | 2018-12-18 | 福建二五区茶业有限公司 | It is a kind of to wither the processing method of white tea using ultraviolet and infrared ray |
| CN109480017A (en) * | 2018-12-17 | 2019-03-19 | 邵阳学院 | A kind of Ramulus et Folium Mussaendae Pubescentis extract and the preparation method and application thereof |
| CN110353062A (en) * | 2019-08-09 | 2019-10-22 | 河北华朗生物科技有限公司 | A kind of formula and its preparation process of cold extraction Ramulus et Folium Mussaendae Pubescentis extract solid beverage |
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| CN110353062A (en) * | 2019-08-09 | 2019-10-22 | 河北华朗生物科技有限公司 | A kind of formula and its preparation process of cold extraction Ramulus et Folium Mussaendae Pubescentis extract solid beverage |
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