CN107372634A - Artemisia annua tobacco vinegar liquid - Google Patents
Artemisia annua tobacco vinegar liquid Download PDFInfo
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- CN107372634A CN107372634A CN201710689181.7A CN201710689181A CN107372634A CN 107372634 A CN107372634 A CN 107372634A CN 201710689181 A CN201710689181 A CN 201710689181A CN 107372634 A CN107372634 A CN 107372634A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/38—Solanaceae [Potato family], e.g. nightshade, tomato, tobacco or chilli pepper
- A01N65/385—Tobacco
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Abstract
The present invention discloses a kind of artemisia annua tobacco vinegar liquid.With 25 times of liquid of artemisia annua tobacco vinegar liquid to 100 times of liquid, 79.17%~100% is reached to the inhibiting rate of Fusarium oxysporum.The soil that root knot nematode disease occurs throughout the year is poured, root-knot nematode inhibiting rate reaches 90%~100%.
Description
Technical field
The invention belongs to botanical material prevention and control crops and Chinese herbal medicine root-knot nematode disease, root rot technical field.It is related to
A kind of vinegar liquid being mixed with artemisia annua stalk and tabacco straw, the vinegar liquid can suppress root-knot nematode and Chinese herbal medicine root rot
It is former.
Background technology
Artemisia annua (Artemisia annua Linn.) is called yellow wormwood artemisia, is the annual herb plant of composite family artemisia, extensively
Distribution each province at home, is Chinese tradition Chinese herbal medicine, its active ingredient qinghaosu in terms of antimalarial with traditional quinine class antimalarial
Medicine has the different mechanism of action.But while artemisia annua serves the contribution performed meritorious deeds never to be obliterated to the medical treatment ＆ health of the mankind, by
Especially rapid in its growth and breeding, environmental adaptability is strong, east China, southern provinces and regions growth by the road, wasteland, hillside, border
Etc., other provinces and regions are also grown in grassland, sylvosteppe, koris, half-desert and gravel matter hillside fields etc., also see saliferous soil
On earth, often largely sprouted in the end of spring and the beginning of summer, and then develop into dominant weed, in order to prevention and control its as weeds to farmland, grassland
Or the harm of forest, also need to research and develop its potential other utilization value.
After tobacco leaf harvest, the cigarette strain residuums such as substantial amounts of cigarette stalk, cigarette root are remained, if removing not in time, it is easy to pollute tobacco
Growing area environment.Tobacco (Nicotiana tabacum) stalk degree of lignification is high, very difficult decomposition, its recycling one
It is directly a problem.There is more serious crop straw burning pollution in tobacco main producing region in recent years.After every tobacco harvest, in order to
Tabacco straw is handled, some areas reach the situation that Dark smoke billows, and this processing mode not only wastes the natural resources of preciousness,
Environmental pollution is caused, also result in Frequent Accidents, to highway, the traffic safety of railway and the landing of commercial air flights safety
Etc. constituting great threat, and serious harm is caused to human health and safety, as a great society problem.In order to solve
Relations problems between human resource, environment and development, effectively and rationally using tabacco straw, its potential value is entered
Row research and development, and reduce the important measures of its negative effect.
Chinese medicine is the medicinal material of Chinese tradition, is the important component of Chinese excellent culture.Chinese herbal medicine belongs to Chinese medicine
Most important part, because the economic position of most Chinese herbal medicines and medicinal part are the roots of Chinese herbal medicine, with medicinal plant kind
Plant area increase sharply and the wild resource of big multi items is domesticated for the large-scale planting of cultivar, butt rot develops
As directly affecting the most serious disease of root yield, quality and commodity value, the value of strong influence Chinese herbal medicine.In furthermore
Herbal medicine disease problem becomes increasingly conspicuous, and because the microorganism group in soil is into extremely complex, therefore to be related to species many for cause of disease, fungi,
Bacterium, nematode can cause Chinese herbal medicine butt rot, and wherein fungal attack is the most serious, and a variety of Chinese herbal medicine roots can be caused rotten
Rotten, such as Fusarium oxysporum (Fusarium oxysporum) can cause matrimony vine, panax japonicus, radix polygonati officinalis, the red sage root, pseudo-ginseng Chinese herbal medicine root
Portion rots, and is one of maximum cause of disease of harmfulness.The long-term incidence of disease of notoginseng root rot is 5%~20%, then up to 70% when serious,
The root of Dahurain angelica root rot incidence of disease is 30% or so, sometimes also up to 80%.In order to control its disease to increase excessively making for chemical pesticide
With, and then the qualified risk of Chinese herbal medicine quality and the friendly to environment are exacerbated, this is as the main of Producing medicinal herbs
Obstacle.
Root-knot nematode (Meloidogyne spp.) is a kind of omnivorousness plant pathogeny line insect of height specialized form, is occurred
The under ground portion of plant.Root knot nematode disease is one of Major Diseases in crops, industrial crops and Chinese herbal medicine cultivation, every year all
There is different degrees of generation.Because the economic position of most Chinese herbal medicines and medicinal part are the roots of Chinese herbal medicine, with medicinal plant
Thing cultivated area increase sharply and the wild resource of most of kinds is domesticated for the large-scale planting of cultivar, medicinal plant
The root-knot nematode disease of thing is continuously increased.In order to control its disease, the excessive use of chemical pesticide, Jin Erjia are increased
The acute qualified risk of Chinese herbal medicine quality and the unfriendly property to environment, this turned into Chinese medicinal herbs production major obstacle it
One.
By literature search, have no and disclose report with identical of the present invention.
The content of the invention
It is an object of the present invention to provide it is a kind of can effectively suppress root-knot nematode, Chinese herbal medicine root-rot cause of disease artemisia annua tobacco vinegar
Liquid product, tabacco straw and artemisia annua stalk is set to be more used.
To reach above-mentioned purpose, technical scheme is as follows：
1. artemisia annua tobacco vinegar liquid.
2. the artemisia annua tobacco vinegar liquid according to technical scheme 1, described artemisia annua tobacco vinegar liquid is by by artemisia annua
The vinegar liquid being prepared after stalk and tabacco straw mixing according to the customary preparation methods of plant vinegar liquid.
3. the artemisia annua tobacco vinegar liquid according to technical scheme 2, described to be mixed into artemisia annua stalk and tabacco straw
By artemisia annua stalk：The mass ratio of tabacco straw is 1~2:1~2 ratio mixing.
4. the artemisia annua tobacco vinegar liquid according to technical scheme 3, described to be mixed into artemisia annua stalk and tabacco straw
By artemisia annua stalk：The mass ratio of tabacco straw is 1:1 ratio mixing.
5. the artemisia annua tobacco vinegar liquid according to technical scheme 3, described to be mixed into artemisia annua stalk and tabacco straw
By artemisia annua stalk：The mass ratio of tabacco straw is 2:1 ratio mixing.
The present invention suppresses notoginseng root rot fungal pathogenses Fusarium oxysporum (Fusarium using artemisia annua tobacco vinegar liquid first
) and root-knot nematode oxysporum.
The soil that root knot nematode disease occurs throughout the year is poured using artemisia annua tobacco vinegar liquid of the present invention, can effectively be pressed down
Root-knot nematode processed, the artemisia annua tobacco vinegar liquid dilute 25 times of liquid to 100 times of liquid, and root-knot nematode inhibiting rate is 90%~100%,
And the soil that root knot nematode disease occurs throughout the year is poured respectively with artemisia annua stalk single dose vinegar liquid or tabacco straw single dose vinegar liquid
Fill, dilute 25 times of liquid to 100 times of liquid in its vinegar liquid, root-knot nematode inhibiting rate is respectively 45%~65%, 25%~49%, this hair
Inhibition ratio artemisia annua stalk single dose vinegar liquid or tabacco straw single dose vinegar liquid of the bright artemisia annua tobacco vinegar liquid to root-knot nematode
It has been respectively increased 53.85%~100%, 104.08%~260%.
Artemisia annua stalk of the present invention：Tabacco straw mass ratio is 2：Suppression of the 1 artemisia annua tobacco vinegar liquid to root-knot nematode
Effect is best, and it dilutes 25 times of liquid to 100 times of liquid, and root-knot nematode inhibiting rate is 100%, and artemisia annua stalk single dose vinegar liquid is dilute
When releasing 100 times or tabacco straw single dose vinegar liquid and diluting 100 times, root-knot nematode inhibiting rate is respectively 45%, 25%, chrysanthemum of the present invention
100 times of liquid of wormwood artemisia tobacco vinegar liquid suppress the inhibition of root-knot nematode than 100 times of liquid of artemisia annua stalk single dose vinegar liquid or tabacco straw
100 times of liquid of single dose vinegar liquid have been respectively increased 122%, 300%.
During artemisia annua tobacco vinegar liquid dilution 25 times of liquid to 100 times of liquid of the present invention, to notoginseng root rot fungal pathogenses point spore reaping hook
Bacterium inhibiting rate reaches 79.17%~100%, dilutes 25 times of liquid to 100 times of liquid to sharp spore reaping hook than artemisia annua stalk single dose vinegar liquid
Bacterium inhibiting rate improves 78.15%~111.77%.25 times of liquid are diluted to 100 times of liquid to sharp spore reaping hook than tabacco straw single dose vinegar liquid
Bacterium inhibiting rate improves 111.12%~139.98%.
With artemisia annua stalk of the present invention：Tabacco straw mass ratio is 2：1 artemisia annua tobacco vinegar liquid suppresses notoginseng root rot
Fungal pathogenses, when diluting 100 times, the inhibiting rate for suppressing notoginseng root rot fungal pathogenses Fusarium oxysporum is 98.61%, and uses chrysanthemum
Wormwood artemisia stalk single dose vinegar liquid and tabacco straw single dose vinegar liquid suppress the inhibiting rate point of notoginseng root rot fungal pathogenses Fusarium oxysporum respectively
Not Wei 44.44%, 37.5%, artemisia annua stalk of the present invention：Tabacco straw mass ratio is 2：1 100 times of artemisia annua tobacco vinegar liquid
Liquid is to the inhibition of notoginseng root rot fungal pathogenses Fusarium oxysporum than 100 times of liquid of artemisia annua stalk single dose vinegar liquid or tabacco straw
100 times of liquid of single dose vinegar liquid have been respectively increased 121.89%, 162.96%.
Compared with artemisia annua stalk single dose vinegar liquid or tabacco straw single dose vinegar liquid, artemisia annua tobacco vinegar liquid of the present invention is to root knot
The suppression of nematode and suppression to Chinese herbal medicine root rot fungal pathogenses generate unexpected technique effect.
Brief description of the drawings
Fig. 1：Prepare the equipment schematic diagram of artemisia annua tobacco vinegar liquid.
Each mark represents successively in figure：1-retort；2-heater；3-drain tap；4-air guide tube valve；5—
Condenser pipe air induction conduit；6-condenser pipe；7-condenser pipe delivery port；The exhaust manifolds of 8-flask with three necks,round bottom；9-round bottom three
Mouth flask；10-round-bottomed flask；11- condensate draining delivery ports；12-refrigeration penstock；13-condenser pipe water inlet pipe；14-cold
Condensate groove；The liquid inlet of 15-flask with three necks,round bottom；16-thermometer.
Embodiment
Following examples are conventional method without specified otherwise.
The preparation of the artemisia annua tobacco vinegar liquid of embodiment 1
First, artemisia annua tobacco vinegar liquid is prepared using the conventional equipment and the customary preparation methods of plant vinegar liquid that prepare vinegar liquid,
Artemisia annua stalk：The mass ratio of tabacco straw is respectively 1:1 or 2:1 or 1:The stalk of 2 ratio mixing prepares artemisia annua tobacco
Vinegar liquid, carry out as follows：
A. artemisia annua stalk and tabacco straw are cut into segment (segment that can be cut into 5cm-8cm) respectively, by artemisia annua straw
Stalk：The mass ratio of tabacco straw is 1:1 or 2:1 or 1:2 ratio, after artemisia annua stalk segment and tabacco straw segment are mixed
It is fitted into retort 1；
B. plus the sealing ring on good retort 1, the lid of retort 1 is installed, tightens its bolt；
C. the drain tap 3 on retort 1 is opened；
D. heater 2 is opened, retort 1 is heated；
E. when thermometer 16 reaches 90 DEG C -100 DEG C, drain tap 3 is closed；
F. refrigeration penstock 12 is opened, opens the air guide tube valve 4 on retort 1；
G. the temperature of heater 2 is controlled, under normal pressure, the temperature of retort 1 is measured using thermometer 16, condensation collects 90 DEG C
~300 DEG C of all cuts；
H. the air guide tube valve 4 closed on retort 1, drain tap 3 is opened, closes heater 2；
I. refrigeration penstock 12 is closed, removes flask with three necks,round bottom 9, the liquid (i.e. vinegar liquid) that flask with three necks,round bottom 9 is collected
For artemisia annua tobacco vinegar liquid of the present invention, it is placed in brown bottle and retains.Artemisia annua stalk：The mass ratio of tabacco straw
Respectively 1:1 or 2:1 or 1:2 ratio mixing respectively obtains three kinds of artemisia annua tobacco vinegar liquids：Artemisia annua stalk：Tabacco straw matter
Amount is than being 1:1 artemisia annua tobacco vinegar liquid, artemisia annua stalk：Tabacco straw mass ratio is 2：1 artemisia annua tobacco vinegar liquid, chrysanthemum
Wormwood artemisia stalk：Tabacco straw mass ratio is 1：2 artemisia annua tobacco vinegar liquids.
The artemisia annua tobacco vinegar liquid of embodiment 2 suppresses the application experiment of root-knot nematode
Root knot nematode disease and the uniform plot of morbidity are occurring throughout the year, is taking the soil of top soil, is well mixed decile
For 6 parts, 6 parts of soil are respectively to handle 1, processing 2, processing 3, processing 4, processing 5, processing 6.3 repetitions are made in each processing.
Processing 1, processing 2 and processing 3 are to suppress root-knot nematode with artemisia annua tobacco vinegar liquid of the present invention to test, processing 1, place
Reason 2 and processing 3 used in artemisia annua tobacco vinegar liquid except that：
Processing 1：Artemisia annua tobacco vinegar liquid used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 1:Load retort 1 after 1 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
Artemisia annua tobacco vinegar liquid is prepared in the preparation method of vinegar liquid.
Processing 2：Artemisia annua tobacco smoke used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 2:Load retort 1 after 1 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
Artemisia annua tobacco vinegar liquid is prepared in the preparation method of vinegar liquid.
Processing 3：Artemisia annua tobacco vinegar liquid used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 1:Load retort 1 after 2 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
Artemisia annua tobacco vinegar liquid is prepared in the preparation method of vinegar liquid.
Processing 4：Only tested with artemisia annua stalk single dose vinegar liquid.
Processing 5：Only tested with tabacco straw single dose vinegar liquid.
The preparation method of artemisia annua stalk single dose vinegar liquid removes the stalk that step a. in embodiment 1 loads retort 1 in processing 4
Simply outside artemisia annua stalk, remaining operation is identical with the preparation method of the artemisia annua tobacco vinegar liquid described in embodiment 1.
The preparation method of tabacco straw single dose vinegar liquid removes the stalk that step a. in embodiment 1 loads retort 1 in processing 5
It is outside tabacco straw, remaining operation is identical with the preparation method of the artemisia annua tobacco vinegar liquid described in embodiment 1.
Processing 1, processing 2, processing 3, processing 4 and processing 5 except vinegar liquid used as described above Bu Tong in addition to, as follows
Carry out experimental implementation：
The vinegar liquid 200ml prepared using above-mentioned preparation method, by 6 dilution gradients of setting, running water is used respectively
It is configured to 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, the solution 5000ml of 150 times of liquid and 200 times of liquid is standby.It will be ready to
Long-term generation root knot nematode disease and the uniform plot of morbidity soil, be sub-packed in flowerpot, plant susceptible root-knot nematode kind with
The tomato of color row 208, (the vinegar liquid amount that each processing is poured is equal) is poured with the vinegar liquid prepared, according to general planting side
Method management.After 8 weeks, the ratio i.e. root knot percentage that the root knot in Tomato Root System accounts for whole root system is investigated.
Processing 6：1 part of soil of decile is loaded on flowerpot, plants the susceptible tomato of root-knot nematode kind Israel 208, according to
General planting method management.After 8 weeks, the ratio i.e. root knot percentage that the root knot in Tomato Root System accounts for whole plant root is investigated
Rate.
Result of the test：The vinegar liquid of different diluted concentrations has inhibition to root-knot nematode, but with the increasing of extension rate
Add, inhibition reduces.Under same extension rate, artemisia annua stalk：Tabacco straw mass ratio is 2：Inhibition when 1 is most
It is good.
Root-knot nematode inhibiting rate %=1- root knot percentage %.
The experimental result of embodiment 2 refers to table 1.
The artemisia annua tobacco vinegar liquid of table 1 suppresses root-knot nematode effect and compared (root knot percentage %)
The artemisia annua tobacco vinegar liquid of embodiment 3 suppresses the application experiment of notoginseng root rot fungal pathogenses
Notoginseng root rot fungal pathogenses used are Fusarium oxysporum (Fusarium oxysporum) in embodiment 3, its point
From identification cultural method with reference to [Fang Zhongda chief editors plants sick research method (third edition) .2004, Beijing：Chinese agriculture publishing house；C
Booth.The genus Fusarium.Commonwealth Agricultura,1971；On the super Fungal identifications handbook of Wei Jing
Sea:Shanghai science tech publishing house, 1979].
Processing 1, processing 2 and processing 3 are tested with described artemisia annua tobacco vinegar liquid, processing 1, processing 2 and place
Reason 3 except that：
Processing 1：Artemisia annua tobacco vinegar liquid used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 1:Load retort 1 after 1 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
The artemisia annua tobacco vinegar liquid that the preparation method of vinegar liquid is prepared.
Processing 2：Artemisia annua tobacco smoke used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 2:Load retort 1 after 1 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
The artemisia annua tobacco vinegar liquid that the preparation method of vinegar liquid is prepared.
Processing 3：Artemisia annua tobacco vinegar liquid used is by artemisia annua straw by artemisia annua stalk segment and tabacco straw segment
Stalk：The mass ratio of tabacco straw is 1:Load retort 1 after 2 ratio mixing, then the artemisia annua tobacco as described in embodiment 1
The artemisia annua tobacco vinegar liquid that the preparation method of vinegar liquid is prepared.
Processing 4：Only tested with artemisia annua stalk single dose vinegar liquid.
Processing 5：Only tested with tabacco straw single dose vinegar liquid.
The preparation method of artemisia annua stalk single dose vinegar liquid removes the stalk that step a. in embodiment 1 loads retort 1 in processing 4
Simply outside artemisia annua stalk, remaining operation is identical with the preparation method of the artemisia annua tobacco vinegar liquid described in embodiment 1.
The preparation method of tabacco straw single dose vinegar liquid removes the stalk that step a. in embodiment 1 loads retort 1 in processing 5
It is outside tabacco straw, remaining operation is identical with the preparation method of the artemisia annua tobacco vinegar liquid described in embodiment 1.
Processing 1, processing 2, processing 3, processing 4 and processing 5 except vinegar liquid used as described above Bu Tong in addition to, as follows
Carry out experimental implementation：
The vinegar liquid prepared using the above method, according to the mass ratio of following vinegar liquid and PDA culture medium, vinegar is prepared respectively
Liquid extension rate is 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, the PDA culture medium containing vinegar liquid of 150 times of liquid and 200 times of liquid,
That is vinegar liquid：Mass ratio=1 of PDA culture medium：24, vinegar liquid：Mass ratio=1 of PDA culture medium：49, vinegar liquid：PDA culture medium
Mass ratio=1：74, vinegar liquid：Mass ratio=1 of PDA culture medium：99, vinegar liquid：Mass ratio=1 of PDA culture medium：149, vinegar liquid：
Mass ratio=1 of PDA culture medium：199.Each vinegar liquid prepares above-mentioned 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times
The PDA culture medium containing vinegar liquid of 6 different vinegar liquid extension rates of liquid and 200 times of liquid, it is each respectively to load a culture dish, each
Culture dish loads 10 milliliters of the PDA culture medium containing vinegar liquid.
Aseptically, the sharp spore sickle identified with the card punch (0.5cm, sterilization treatment) of same model in culture purified
The colony edge punching of knife bacterium, the bacterium colony punched with scalpel difference picking card punch is in the same size, the consistent sharp spore of activity
Sickle-like bacteria bacterium colony, it is transferred in the above-mentioned culture dish equipped with the PDA culture medium containing vinegar liquid, 1 sharp spore sickle of each culture dish switching
Knife bacterium bacterium colony, covers culture dish lid, is put in incubator culture 5 days, measurement record colony growth diameter, calculates inhibition.
Processing 6 is blank control experiment.Processing 6：Aseptically, as stated above transfer 1 Fusarium oxysporum bacterium
Fall in the culture dish for only filling 10 milliliters of PDA culture mediums, cover culture dish lid, be directly put in incubator culture 5 days, measurement note
Record colony growth diameter.
3 repetitions are made in each processing.
Result of the test：The vinegar liquid of different diluted concentrations has inhibition to Fusarium oxysporum, but with extension rate
Increase, inhibition reduce.Under same extension rate, artemisia annua stalk：Tabacco straw mass ratio is 2：Artemisia annua cigarette when 1
Careless vinegar liquid inhibition is best.
The experimental result of embodiment 3 refers to table 2.
The artemisia annua tobacco vinegar liquid of table 2 suppresses notoginseng root rot fungal pathogenses effect and compared (colony diameter cm)
Fusarium oxysporum inhibiting rate calculation formula：
Fusarium oxysporum inhibiting rate=[colony diameter-untreated colony diameter ︱/untreated bacterium colony of ︱ processing is straight
Footpath] X100%.
Table 2 shows：
Processing 1：Artemisia annua stalk：Tabacco straw mass ratio is 1：1 artemisia annua tobacco vinegar liquid 25 times of liquid of dilution, 50 times
Liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200 times of liquid are respectively 88.89% to Fusarium oxysporum inhibiting rate, 88.89%,
87.5%th, 83.33%, 83.33%, 81.94%.
Processing 2：Artemisia annua stalk：Tabacco straw mass ratio is 2：1 artemisia annua tobacco vinegar liquid 25 times of liquid of dilution, 50 times
Liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200 times of liquid are respectively 100% to Fusarium oxysporum inhibiting rate, 100%,
98.61%th, 98.61%, 97.22%, 97.22%.
Processing 3：Artemisia annua stalk：Tabacco straw mass ratio is 1：2 artemisia annua tobacco vinegar liquid 25 times of liquid of dilution, 50 times
Liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200 times of liquid are respectively 86.11% to Fusarium oxysporum inhibiting rate, 86.11%,
86.11%th, 79.17%, 75.00%, 72.22%.
Processing 4：Artemisia annua stalk single dose vinegar liquid dilutes 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200
Times liquid is respectively 47.22%, 47.22%, 47.22%, 44.44%, 44.44%, 37.50% to Fusarium oxysporum inhibiting rate.
Processing 5：Tabacco straw single dose vinegar liquid dilutes 25 times of liquid, 50 times of liquid, 75 times of liquid, 100 times of liquid, 150 times of liquid and 200 times
Liquid is respectively 41.67%, 41.67%, 41.67%, 37.50%, 26.39%, 23.61% to Fusarium oxysporum inhibiting rate.
When artemisia annua tobacco vinegar liquid dilutes 25 times of liquid to 100 times of liquid, notoginseng root rot fungal pathogenses Fusarium oxysporum is suppressed
Rate reaches 79.17%~100%, and artemisia annua stalk single dose vinegar liquid dilutes 25 times of liquid and Fusarium oxysporum is suppressed to 100 times of liquid
Rate is 44.44%~47.22%, when artemisia annua tobacco vinegar liquid dilutes 25 times of liquid to 100 times of liquid, than artemisia annua stalk single dose vinegar liquid
Dilute 25 times of liquid and 78.15%~111.77% is improved to Fusarium oxysporum inhibiting rate to 100 times of liquid.And tabacco straw single dose vinegar liquid
It is 37.50%~41.67% to Fusarium oxysporum inhibiting rate to dilute 25 times of liquid to 100 times of liquid, artemisia annua tobacco vinegar liquid dilution 25
During times liquid to 100 times of liquid, dilute 25 times of liquid than tabacco straw single dose vinegar liquid and Fusarium oxysporum inhibiting rate is improved to 100 times of liquid
111.12%~139.98%.
Artemisia annua stalk：Tabacco straw mass ratio is 2：1 artemisia annua tobacco vinegar liquid is to notoginseng root rot fungal pathogenses point spore
Sickle-like bacteria inhibiting rate effect is best, reach 98.61% to notoginseng root rot fungal pathogenses Fusarium oxysporum inhibiting rate effect~
100%, when it dilutes 100 times of liquid, 98.61% is reached to notoginseng root rot fungal pathogenses Fusarium oxysporum inhibiting rate effect, and use
100 times of liquid of 100 times of liquid of artemisia annua stalk single dose vinegar liquid or tabacco straw single dose vinegar liquid are respectively to notoginseng root rot fungal pathogenses point spore
Sickle-like bacteria inhibiting rate is 44.44%, 37.5%, and Fusarium oxysporum inhibiting rate is carried than artemisia annua stalk single dose 100 times of liquid of vinegar liquid
It is high by 121.89%；162.96% is improved to Fusarium oxysporum inhibiting rate than 100 times of liquid of tabacco straw single dose vinegar liquid.
Claims (5)
1. artemisia annua tobacco vinegar liquid.
2. artemisia annua tobacco vinegar liquid according to claim 1, it is characterised in that：The artemisia annua tobacco vinegar liquid be pass through by
The vinegar liquid being prepared after artemisia annua stalk and tabacco straw mixing according to the customary preparation methods of plant vinegar liquid.
3. artemisia annua tobacco vinegar liquid according to claim 2, it is characterised in that：It is described by artemisia annua stalk and tabacco straw
It is mixed into by artemisia annua stalk：The mass ratio of tabacco straw is 1~2:1~2 ratio mixing.
4. artemisia annua tobacco vinegar liquid according to claim 3, it is characterised in that：It is described by artemisia annua stalk and tabacco straw
It is mixed into by artemisia annua stalk：The mass ratio of tabacco straw is 1:1 ratio mixing.
5. artemisia annua tobacco vinegar liquid according to claim 3, it is characterised in that：It is described by artemisia annua stalk and tabacco straw
It is mixed into by artemisia annua stalk：The mass ratio of tabacco straw is 2:1 ratio mixing.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN111838195A (en) * | 2020-07-23 | 2020-10-30 | 王婉君 | Novel environment-friendly plant source artemisia annua tobacco vinegar liquid for agriculture |
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