CN107372104A - A kind of detoxicated ginger takes off the rapid propagation method of bacterium - Google Patents
A kind of detoxicated ginger takes off the rapid propagation method of bacterium Download PDFInfo
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- CN107372104A CN107372104A CN201710454638.6A CN201710454638A CN107372104A CN 107372104 A CN107372104 A CN 107372104A CN 201710454638 A CN201710454638 A CN 201710454638A CN 107372104 A CN107372104 A CN 107372104A
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- ginger
- bacterium
- hardening
- propagation method
- rapid propagation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention discloses the rapid propagation method that a kind of detoxicated ginger takes off bacterium, concretely comprises the following steps：A, carry out disinfection processing after cutting sprouting from ginger block to sprouting, uses aseptic water washing material 3 times after having sterilized, the aseptic explant of 0.5~1.0mm or so sizes, as sterile nontoxic bud are then asked for by sterile working；B, on superclean bench, the sterile nontoxic bud of acquirement is seeded in inducing culture, PH5.8~6.0, through one month, forms small ginger；C, the small ginger of acquirement is transferred to proliferated culture medium, PH5.8~6.0, treats that small ginger directly forms seedling；D, treat that seedling grows to 5 cms, remove proliferated culture medium and carry out hardening, require more than 80% humidity in the week of hardening first, then gradually reduced in one week up to nature, hardening terminates after one month, then transplants crop field again, forms kind of a ginger.It is of the invention simple nuisanceless, greatly shorten the production cycle, reduce production cost, significant effect.
Description
Technical field
The present invention relates to technical field of plant propagation, more particularly to a kind of detoxicated ginger to take off the rapid propagation method of bacterium.
Background technology
Ginger has very high medicinal, edible and economic value, is one of important industrial crops in China.In recent years, ginger
Consumption figure is increasing, and ginger productivity effect increasingly increases, and cultivated area expands year by year, throughout all parts of the country.Due to ginger not
Bloom or seldom bloom, it is impossible to carry out breeding using generative propagation, the breeding and preservation of its germ plasm resource are relied primarily on kind of a ginger
Make propagating materials and carry out field planting every year.Not only breeding coefficient is low, costly for this, due also to natural calamity and pest and disease damage
Harm is likely to result in the loss of resource material.Nourished and generated plus long-term, oneself causes the kind sexual involution of ginger and susceptible tight
Weight, causes quality and yield to decline, quality deterioration, and resistance declines, and ginger production is constituted a serious threat, meanwhile, ginger is easy
The withered rot of disease, especially Jiang Qing, also known as ginger plague are propagated by band disease ginger, have generation in each ginger producing region in the world, sternly
The production and development of ginger are constrained again.Preferable bactericidal agent and antibacterial kind are there is no at present, can only be with cultural control measure such as
From anti-(resistance to) property kind, carry out based on kind of ginger pharmacy disinfection and soil disinfection, crop rotation etc., being aided with chemical control, but
Effect is undesirable.
Vitro Quick Reproduction technology has the advantages that not by season and environmental restrictions, and breeding coefficient is high, and speed is fast.Utilize
Tissue culture takes off bacterium technology, obtains de- bacterium ginger seedling, can overcome kind of a sexual involution, improve degeneration-resistant resistance, effective controlling plant diseases.It is de-
Bacterium ginger kind is divided into three generations i.e. original silkworm egg, original seed and production kind, and production kind can apply in production, and the term of validity is to be needed after 3 years, 3 years
Change 1 time.By that by de- bacterium the amount of increase in production of ginger can be made to reach more than 30%, and seedling growth is fast, growing way is prosperous, resistance；
The ginger appearance luster produced is good.So yield can not only be improved by de- bacterium, while the quality and price of ginger can be improved.
It is existing also to have some ginger rapid propagation methods, such as the step of ginger one that China Patent No. is 201310301668.5
Into detoxic seedling rapid propagation method, which employs the MS culture mediums of routine, the formula of the MS culture mediums is (unit：mg/L)：
NH4NO3 1650
KNO3 1900
CaCl2·2H2O 440
MgSO4·7H2O 370
KH2PO4 170
KI 0.83
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA·2H2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2.0.
But the propagation method is still relatively complicated, has much room for improvement.
The content of the invention
It is an object of the invention to overcome above-mentioned the deficiencies in the prior art, there is provided one kind using ginger bud progress detoxicated ginger,
The tissue-culturing rapid propagation production method of de- bacterium.
To achieve these goals, the present invention adopts the following technical scheme that：
A kind of detoxicated ginger takes off the rapid propagation method of bacterium, concretely comprises the following steps：
A, carry out disinfection processing after cutting sprouting from ginger block to sprouting, uses aseptic water washing material 3 times after having sterilized, then
The aseptic explant of 0.5~1.0mm or so sizes is asked for by sterile working, as sterile nontoxic bud；
B, on superclean bench, the sterile nontoxic bud of acquirement is seeded in inducing culture, inducing culture is：Improvement
MS+6-BA2mg/L+NAA0.5mg/L+Sugar30g/L+ agar 7.5g/L, PH5.8~6.0, through one month, form small ginger；
C, the small ginger of acquirement is transferred to proliferated culture medium, proliferated culture medium is：Improve MS+6-BA1mg/L+
NAA1mg/L+Sugar30g/L, PH5.8~6.0, treat that small ginger directly forms seedling；The step can both breed, while again may be used
With long root, long root this step can be saved, forming seedling through one step culture directly forms seedling；
D, treat that seedling grows to 5 cms, remove proliferated culture medium and carry out hardening, require more than 80% in the week of hardening first
Humidity, then gradually reduced in one week up to nature, hardening terminates after one month, then transplants crop field again, forms kind
Ginger.
The formula of improvement MS in the step B and C is (unit：mg/L)：
KNO3 3800
CaCl2·2H2O 880
MgSO4·7H2O 370
KH2PO4 170
KI 0.83
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA·2H2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2.0.
The disinfectant that disinfecting in the step A uses is 75% alcohol or 30% bleaching powder, is disinfected
Process is carried out in the bottle for have lid, is shaken bottle and is carried out disinfection.
The sterilized water is the distilled water by autoclave sterilization.
The beneficial effects of the invention are as follows：It is nuisanceless using simple, the production cycle is highly shortened, reduces and is produced into
This, significant effect.
Embodiment
Below by embodiment, the invention will be further described：
A kind of detoxicated ginger takes off the rapid propagation method of bacterium, concretely comprises the following steps：
During the annual ginger beginning of spring in spring germination (ginger germination phase), operated preferably when combining and plant to ginger, bar
When part does not allow, as long as ginger has sprouting.
A, carry out disinfection processing after cutting sprouting from ginger block to sprouting, disinfects the alcohol that the disinfectant used is 75%
Or 30% bleaching powder, disinfect process and carried out in the bottle for have lid, shake bottle and carry out disinfection, because surface attachment
Bubble often makes sprouting not reach disinfectant and then as the pollution sources in the culture medium in later stage, and bubble can be reduced by shaking bottle
Attachment.
Aseptic water washing material is used after having sterilized 3 times, the sterilized water is the distilled water by autoclave sterilization, then
The aseptic explant of 0.5~1.0mm or so sizes is asked for by sterile working, as sterile nontoxic bud；
B, on superclean bench, the sterile nontoxic bud of acquirement is seeded in inducing culture, inducing culture is：Improvement
MS+6-BA2mg/L+NAA0.5mg/L+Sugar30g/L+ agar 7.5g/L, PH5.8~6.0, through one month, form small ginger；
C, the small ginger of acquirement is transferred to proliferated culture medium, proliferated culture medium is：Improve MS+6-BA1mg/L+
NAA1mg/L+Sugar30g/L, PH5.8~6.0, treat that small ginger directly forms seedling；The step can both breed, while again may be used
With long root, long root this step can be saved, forming seedling through one step culture directly forms seedling, and the proliferated culture medium not only eliminates agar, uses
Fluid nutrient medium, reduce by 20% production cost, considerable benefit；And appreciation rate is increased by 1 times, specially each bud monthly may be used
To produce 5 sproutings, by this calculating, 12 months 1 year, a bud can produce 2 hundred million.
D, treat that seedling grows to 5 cms, remove proliferated culture medium and carry out hardening, require more than 80% in the week of hardening first
Humidity, then gradually reduced in one week up to nature, hardening terminates after one month, then transplants crop field again, forms kind
Ginger.
The formula of improvement MS in the step B and C is (unit：mg/L)：
KNO3 3800
CaCl2·2H2O 880
MgSO4·7H2O 370
KH2PO4 170
KI 0.83
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA·2H2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2.0.
Incubation coordinates Corticosteroids using improvement MS, not only overcomes the browning problem in ginger incubation,
Make ginger can be with forming seedling through one step culture again.KNO in the improvement MS of the present invention3And CaCl2·2H2O amount doubles, and eliminates NH4NO3,
Other are as the MS culture mediums of routine.
The foregoing is only presently preferred embodiments of the present invention, be not intended to limit the invention, it is all the present invention spirit and
Within principle, any modification, equivalent substitution and improvements made etc., it should be included in the scope of the protection.
Claims (4)
1. a kind of detoxicated ginger takes off the rapid propagation method of bacterium, it is characterised in that concretely comprises the following steps：
A, carry out disinfection processing after cutting sprouting from ginger block to sprouting, aseptic water washing material is used 3 times after having sterilized, then by nothing
Bacterium operates the aseptic explant that ask for 0.5~1.0mm or so sizes, as sterile nontoxic bud；
B, on superclean bench, the sterile nontoxic bud of acquirement is seeded in inducing culture, inducing culture is：Improve MS+
6-BA2mg/L+NAA0.5mg/L+Sugar30g/L+ agar 7.5g/L, PH5.8~6.0, through one month, form small ginger；
C, the small ginger of acquirement is transferred to proliferated culture medium, proliferated culture medium is：Improve MS+6-BA1mg/L+NAA1mg/L+
Sugar30g/L, PH5.8~6.0, treat that small ginger directly forms seedling；
D, treat that seedling grows to 5 cms, remove proliferated culture medium and carry out hardening, require that more than 80% is wet the week of hardening first
Degree, then gradually reduced in one week up to nature, hardening terminates after one month, then transplants crop field again, forms kind of a ginger.
2. a kind of detoxicated ginger as claimed in claim 1 takes off the rapid propagation method of bacterium, it is characterised in that in the step B and C
Improvement MS formula be (unit：mg/L)：
KNO3 3800
CaCl2·2H2O 880
MgSO4·7H2O 370
KH2PO4 170
KI 0.83
H3BO3 6.2
MnSO4·4H2O 22.3
ZnSO4·7H2O 8.6
Na2MoO4·2H2O 0.25
CuSO4·5H2O 0.025
CoCl2·6H2O 0.025
FeSO4·7H2O 27.8
Na2-EDTA·2H2O 37.3
Inositol 100
Nicotinic acid 0.5
Puridoxine hydrochloride 0.5
Thiamine hydrochloride 0.1
Glycine 2.0.
3. a kind of detoxicated ginger as claimed in claim 1 or 2 takes off the rapid propagation method of bacterium, it is characterised in that in the step A
Disinfect the alcohol or 30% bleaching powder that the disinfectant that uses is 75%, disinfect process and enter in the bottle for have lid
OK, bottle is shaken to carry out disinfection.
4. a kind of detoxicated ginger as claimed in claim 3 takes off the rapid propagation method of bacterium, it is characterised in that the sterilized water is warp
Cross the autoclaved distilled water of high temperature.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771725A (en) * | 2020-07-31 | 2020-10-16 | 潍坊兴旺生物种业有限公司 | Optimized ginger virus-free seedling regeneration propagation method and development of industrialization process thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144565A (en) * | 2011-03-03 | 2011-08-10 | 中国药科大学 | Method for cultivating ginger-free viruses and cultivating virus-free seedlings by means of propagating and rooting synchronously |
| CN102939903A (en) * | 2012-11-23 | 2013-02-27 | 绍兴文理学院 | Ginger detoxification, sterilization and rapid propagation method |
| CN103348917A (en) * | 2013-07-18 | 2013-10-16 | 湖北蔬谷农业科技有限公司 | Rapid propagation method of ginger virus-free seedlings by one step |
-
2017
- 2017-06-15 CN CN201710454638.6A patent/CN107372104A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102144565A (en) * | 2011-03-03 | 2011-08-10 | 中国药科大学 | Method for cultivating ginger-free viruses and cultivating virus-free seedlings by means of propagating and rooting synchronously |
| CN102939903A (en) * | 2012-11-23 | 2013-02-27 | 绍兴文理学院 | Ginger detoxification, sterilization and rapid propagation method |
| CN103348917A (en) * | 2013-07-18 | 2013-10-16 | 湖北蔬谷农业科技有限公司 | Rapid propagation method of ginger virus-free seedlings by one step |
Non-Patent Citations (1)
| Title |
|---|
| 刘振伟等: "姜组织培养研究进展", 《中国蔬菜》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111771725A (en) * | 2020-07-31 | 2020-10-16 | 潍坊兴旺生物种业有限公司 | Optimized ginger virus-free seedling regeneration propagation method and development of industrialization process thereof |
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Application publication date: 20171124 |
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