CN107365655A - A kind of method that apple mixes juice fermented soy dry type fermented wine - Google Patents
A kind of method that apple mixes juice fermented soy dry type fermented wine Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/02—Preparation of other alcoholic beverages by fermentation
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Abstract
本发明公开了一种苹果混汁发酵酿造干型发酵酒的方法;该方法使用国光、澳洲青苹和嘎啦三种苹果分别破碎后,按一定比例混合后压榨取汁,并与压榨果渣按比例混合后进行发酵,使发酵后的酒体具有适宜的香气和酸度。在原料处理步骤中用1:1的果汁和果渣混合,使苹果果皮中的香味能够很好地溶入苹果汁中。同时添加低温浓缩的压榨果汁和破碎后的未成熟青果与苹果皮肉混合进行低温浸渍发酵,显著提高了酒体厚重感。并在浸渍期添加复合酶显著提高了果皮中风味前体物质溶出率和分解,结合后期增香酵母的低温慢速浸渍发酵,显著增加了苹果酒中游离香气成分物质的含量。最后对部分成品酒进行蒸馏,将蒸馏酒再次加入成品酒中,解决了酒体风味差的问题。
The invention discloses a method for fermenting dry-type fermented wine by mixing apple juice. In the method, three kinds of apples, Guoguang, Granny Smith and Gala, are respectively crushed, mixed according to a certain proportion, squeezed to obtain juice, and mixed with squeezed pomace. Fermentation is carried out after mixing in proportion, so that the body of the fermented wine has suitable aroma and acidity. In the raw material processing step, a 1:1 mixture of fruit juice and pomace is used, so that the aroma in the apple peel can be well dissolved in the apple juice. At the same time, low-temperature concentrated squeezed juice and crushed immature green fruit are mixed with apple skin for low-temperature maceration and fermentation, which significantly improves the body of the wine. The addition of complex enzymes during the maceration period significantly improved the dissolution rate and decomposition of flavor precursors in the peel, combined with the low-temperature and slow maceration fermentation of aromatizing yeast in the later stage, significantly increased the content of free aroma components in cider. Finally, part of the finished wine is distilled, and the distilled wine is added to the finished wine again, which solves the problem of poor flavor of the wine.
Description
技术领域technical field
本发明属于苹果酒风味增强技术与酿造工艺领域,具体涉及一种苹果混汁发酵酿造干型发酵酒的方法。The invention belongs to the field of cider flavor enhancement technology and brewing technology, and in particular relates to a method for brewing dry fermented wine by fermenting mixed apple juice.
背景技术Background technique
苹果是我国的第一大水果,是我国最具优势的农产品,种植分布非常广泛。从1993年开始,我国苹果在栽培面积和产量上就已经位居世界首位。2016年全国苹果种植面积为246.69万公顷(3700万亩),全国苹果总产量为3405万吨左右,占到世界苹果产量一半多。由于苹果产量巨大,已经远远超出了国内的最大消费量,而苹果的对外出口又仅限于少数的一些产地,大多数果农只能依赖国内市场,并不能根本上解决滞销问题。苹果采摘销售后85%供人鲜食,我国苹果虽然品质尚佳,但由于供大于求,苹果价格一路走低,增加了果农的负担,也没能创造出足够的经济效益。因此,对其进行深加工以提高苹果附加值就显得极为重要。Apple is the largest fruit in my country and the most advantageous agricultural product in China, with a wide distribution of planting. Since 1993, my country's apple cultivation area and output have ranked first in the world. In 2016, the national apple planting area was 2.4669 million hectares (37 million mu), and the total national apple production was about 34.05 million tons, accounting for more than half of the world's apple production. Due to the huge output of apples, which has far exceeded the maximum domestic consumption, and the export of apples is limited to a few production areas, most fruit farmers can only rely on the domestic market, which cannot fundamentally solve the problem of slow sales. 85% of the apples are picked and sold for fresh consumption. Although the quality of apples in my country is still good, due to the oversupply, the price of apples has been falling all the way, which has increased the burden on fruit farmers and failed to create sufficient economic benefits. Therefore, it is extremely important to further process it to increase the added value of apples.
苹果酒是苹果深加工的主要产品之一,是仅次于葡萄酒的世界第二大果酒,又称西打酒(Cider),起源于法国西北部寒冷的诺曼底地区,流行于欧、美、澳等国家,是国际饮料酒市场中经久不衰的热点。与葡萄酒相比,苹果酒的生产无季节性限制,周期短,价位低于葡萄酒,比葡萄酒的营养成分更加丰富,口感又较为醇和。它富含多种维生素、氨基酸、钙、铁、钾等营养成分,以及其它酒类所没有的苹果酸、丙酮酸等有机酸,可以调整人体的新陈代谢,促进血液循环。Apple cider is one of the main products of deep processing of apples. It is the second largest fruit wine in the world next to wine. It is also called Cider. It originated in the cold Normandy region in northwest France and is popular in Europe, America, Australia, etc. The country is an enduring hotspot in the international beverage wine market. Compared with wine, the production of cider has no seasonal restrictions, the production cycle is short, the price is lower than that of wine, and it is more nutritious than wine, and the taste is more mellow. It is rich in vitamins, amino acids, calcium, iron, potassium and other nutrients, as well as organic acids such as malic acid and pyruvic acid that are not found in other wines, which can adjust the body's metabolism and promote blood circulation.
目前,苹果酒在国外的苹果深加工中占有很大比例,国外苹果酒产量在2011年左右已达50万吨,而且以每年10%的速率增长。而我国在这一领域中尚处于起步阶段,我国果酒生产企业虽然早已超过100家,但生产能力大都在1000吨以下,万吨以上生产规模的企业极少,导致我国苹果酒在全球市场上占有份额较低。其根本原因在于我国苹果酒生产工艺仍较为落后,所产苹果酒品质欠佳。目前国内苹果酒的生产厂家大多采用陈旧工艺和常规葡萄酒发酵酵母进行生产。苹果酒酵母菌种选育、果汁原料褐变、风味流失严重、成品酒口感寡淡等工艺技术难题在很大程度上影响了苹果酒的品质,而苹果酒所含成分又十分复杂,其中澄清处理、发酵等因素也直接关系到苹果酒的稳定性和贮存时间。缺乏成熟优质的苹果酒酵母菌种,部分科研成果仅停留在试验研究阶段,生产工艺中的关键技术仍不成熟是我国苹果酒产业的现状。At present, cider occupies a large proportion in the deep processing of apples in foreign countries. The output of foreign cider has reached 500,000 tons around 2011, and it is growing at a rate of 10% per year. However, my country is still in its infancy in this field. Although there are more than 100 fruit wine production enterprises in my country, most of them have a production capacity of less than 1,000 tons, and there are very few enterprises with a production scale of more than 10,000 tons. Lower share. The fundamental reason is that the production technology of cider in my country is still relatively backward, and the quality of cider produced is not good. At present, domestic cider manufacturers mostly use old technology and conventional wine fermentation yeast for production. Breeding of cider yeast strains, browning of fruit juice raw materials, serious flavor loss, and weak taste of finished wine have largely affected the quality of cider, and the ingredients contained in cider are very complicated. Factors such as handling and fermentation are also directly related to the stability and storage time of cider. The lack of mature and high-quality cider yeast strains, some scientific research results only stay in the experimental research stage, and the key technologies in the production process are still immature are the current status of my country's cider industry.
我国市场上已有的苹果酒风味和口感较差,不能充分体现其果酒特色,酒体普遍氧化褐变严重、香气淡、口感单薄缺乏回味感与厚重感,直接降低了我国消费者对苹果酒的认可度。本发明针对现有的苹果酒酿造与酒体品质问题进行了技术创新,使用了不同品种的苹果按比例混合,并将其青汁与果皮渣按一定比例混合,同时添加低温浓缩汁和青果果肉进行低温浸渍,而后采用增香酵母进行低温长时浸渍发酵;在浸渍期并添加复合酶(包括纤维素酶、果胶酶及β-葡萄糖苷酶等);从而创立了一种苹果酒风味增强技术与酿造新工艺。The existing cider flavor and taste in the Chinese market are relatively poor, and cannot fully reflect the characteristics of its fruit wine. The wine body is generally severely oxidized and browned, the aroma is light, the taste is thin, and the lack of aftertaste and heavy feeling directly reduces the consumer's perception of cider in our country. degree of recognition. The present invention has carried out technological innovation aiming at the problems of existing cider brewing and wine body quality, using different varieties of apples to mix in proportion, and mixing their green juice and fruit pomace in a certain proportion, and adding low-temperature concentrated juice and green fruit pulp at the same time Carry out low-temperature maceration, and then use aroma-enhancing yeast for low-temperature and long-term maceration fermentation; add complex enzymes (including cellulase, pectinase and β-glucosidase, etc.) during the maceration period; thus creating a cider flavor enhancement Technology and brewing new technology.
发明内容Contents of the invention
针对现有技术中存在的问题,本发明的目的在于提供一种苹果混汁发酵酿造干型发酵酒的方法,其有效解决了现有技术中存在的问题。Aiming at the problems existing in the prior art, the object of the present invention is to provide a method for brewing dry fermented wine by fermenting mixed apple juice, which effectively solves the problems existing in the prior art.
为实现上述目的,本发明采用以下技术方案:To achieve the above object, the present invention adopts the following technical solutions:
一种苹果混汁发酵酿造干型发酵酒的方法,所述方法包括如下步骤:A method for brewing dry fermented wine by fermenting apple mixed juice, said method comprising the steps of:
1)原料处理:将成熟的国光、澳洲青苹和嘎啦三种苹果,清洗干净后,用冷风吹干表面水份,使用栏式压榨机进行破碎,三种苹果分别破碎后按照设定比例的重量比混合压榨得苹果清汁,之后将此清汁和果渣按1:1的重量比进行混合得混汁,在破碎的过程中按50-70mg/L的比例加入用苹果汁溶解的偏重亚硫酸钾以及20-40mg/L的Vc,同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护;1) Raw material processing: After cleaning the mature Guoguang, Granny Smith and Gala apples, dry the surface moisture with cold air, and crush them with a column press. The three kinds of apples are crushed separately and according to the set ratio The weight ratio is mixed and pressed to obtain clear apple juice, and then the clear juice and pomace are mixed at a weight ratio of 1:1 to obtain a mixed juice. During the crushing process, 50-70 mg/L of apple juice is added. Potassium sulfate and 20-40mg/L Vc, and at the same time add 0.5kg per thousand liters of mixed juice to fill with carbon dioxide gas for protection;
2)低温浓缩果汁:使用低温真空浓缩装置,按浓缩比例5:1,转速30rpm,在65℃条件下,对三种苹果混汁进行浓缩处理;2) Low-temperature concentrated fruit juice: use a low-temperature vacuum concentration device to concentrate the three kinds of apple mixed juices at a concentration ratio of 5:1 and a rotation speed of 30 rpm at 65°C;
3)带皮浸渍:在混汁中加入低温浓缩果汁和经破碎的未成熟青果;之后加入活化的β-葡萄糖苷酶、纤维素酶和增香果胶酶,加入量为50mg/L混汁;在二氧化碳保护的条件下,在3-8℃下低温浸渍36-48h;3) Dipping with skin: Add low-temperature concentrated fruit juice and crushed immature green fruit to the mixed juice; then add activated β-glucosidase, cellulase and aroma-enhancing pectinase, the addition amount is 50mg/L mixed juice ;Under the condition of carbon dioxide protection, low temperature dipping at 3-8°C for 36-48h;
4)成分调整:测定苹果混汁糖度,按照酿造12°苹果干酒的标准,加糖调整混汁的糖度至23%;4) Ingredient adjustment: measure the sugar content of the mixed apple juice, according to the standard for brewing 12° apple dry wine, add sugar to adjust the sugar content of the mixed juice to 23%;
5)增香酵母种子罐的建立:步骤3)中带皮浸渍24h后,取70-100L的混汁于小罐中备用,将活化干增香酵母进行活化;5) Establishment of aroma-enhancing yeast seed tank: after soaking with skin for 24 hours in step 3), take 70-100L of mixed juice in a small tank for later use, and activate the dried aroma-enhancing yeast;
6)接种增香酵母:按比例将种子罐中的混汁加入至发酵罐,之后立即进行一次流量为4t/h的开放式循环;6) Inoculation of aroma-enhancing yeast: add the mixed juice in the seed tank to the fermenter in proportion, and then immediately perform an open cycle with a flow rate of 4t/h;
7)发酵控制:发酵期间采用冷却装置保持发酵液温度为12-15℃,记录发酵液温度和比重;并根据比重进行封闭式循环;比重降至为0.992时视为发酵终止;主发酵结束后,18-20℃浸渍24-48h;7) Fermentation control: During the fermentation period, a cooling device is used to keep the temperature of the fermentation broth at 12-15°C, and the temperature and specific gravity of the fermentation broth are recorded; and a closed cycle is carried out according to the specific gravity; when the specific gravity drops to 0.992, the fermentation is considered to be terminated; after the main fermentation , 18-20°C impregnation for 24-48h;
8)酒渣分离:浸渍结束后,反复进行皮渣分离,至分离到的原酒中无大块悬浮物时即可停止离心;8) Separation of wine residues: after the immersion, the separation of the skins and residues is repeated, and the centrifugation can be stopped when there are no large suspended solids in the separated original wine;
9)蒸馏酒的处理与添加:使用低温真空浓缩装置对部分苹果原酒进行蒸馏,取蒸馏酒酒身添加至苹果原酒中,蒸馏酒的添加量根据苹果酒最终酒度要求添加;9) Treatment and addition of distilled wine: Use a low-temperature vacuum concentration device to distill part of the original apple wine, take the body of the distilled wine and add it to the original apple wine, and the amount of distilled wine added is based on the final alcohol content of the apple wine;
10)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合;在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变;下胶处理后8d-10d形成沉渣,并去除;10) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body; during this period, add 20g/t potassium metabisulfite and Vc respectively to inhibit bacteria Pollution and prevention of browning; 8d-10d after gluing treatment, sediment is formed and removed;
11)稳定性处理:对发酵罐进行降温处理,保持发酵罐内酒体温度为-4.5℃,维持10-14d;11) Stability treatment: cooling down the fermenter to keep the wine body temperature at -4.5°C for 10-14 days;
12)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和菌类。12) Filtration: use cross-flow filtration to filter the wine body to remove sediment and fungi.
进一步,步骤1)中所述设定比例为国光70%、澳洲青苹15%、嘎啦苹果15%。Further, the set proportions in step 1) are 70% for Guoguang, 15% for Granny Smith apples and 15% for Gala apples.
进一步,步骤3)中混汁与低温浓缩汁的添加比例为6:1-5:1;未成熟青果的果龄确定在8月份,落果收集后冷冻,加入量为100g/L混汁;步骤3)中加入活化的β-葡萄糖苷酶、纤维素酶和增香果胶酶的重量比为1:2:2。Further, in step 3), the ratio of mixed juice to low-temperature concentrated juice is 6:1-5:1; the fruit age of immature green fruit is determined in August, and the fallen fruit is collected and frozen, and the addition amount is 100g/L mixed juice; step 3) The weight ratio of activated β-glucosidase, cellulase and aroma-enhancing pectinase is 1:2:2.
进一步,步骤4)中加入的糖为蔗糖和果糖,所述蔗糖和果糖的加入重量比为2:1。Further, the sugar added in step 4) is sucrose and fructose, and the weight ratio of sucrose and fructose is 2:1.
进一步,步骤5)中的活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16-18h。Further, the activation method in step 5) is: add 600-800mg/L mixed juice; first dissolve the dry aroma-enhancing yeast in pure water at 36-38°C in a ratio of 1:10, stir well, and activate for 15 minutes; take Add some mixed apple juice to the dissolved aroma-enhancing yeast several times, measure the temperature of the activation solution while adding, take the temperature of the mixed juice to drop 5°C as the breakpoint, and continue to activate for 15 minutes. When the temperature difference between the activation solution and the mixed juice is Stop adding the mixed juice at 3°C; mix the activation solution evenly into the 70-100L mixed juice prepared earlier, immediately perform an open cycle with a flow rate of 2t/h, then close the tank and incubate at 28°C for 16-18h.
进一步,步骤6)中种子罐中的混汁与发酵罐中的混汁混合的比例是1:10。Further, the mixing ratio of the mixed juice in the seed tank and the mixed juice in the fermenter in step 6) is 1:10.
进一步,步骤7)中根据比重进行封闭式循环具体为:比重大于1.001时,每2-3h进行一次流量为4-5t/h的封闭式循环,比重小于1.001时每8h进行一次流量为4-5t/h封闭式循环。Further, in step 7), the closed circulation according to the specific gravity is specifically: when the specific gravity is greater than 1.001, a closed circulation with a flow rate of 4-5t/h is carried out every 2-3h, and a flow rate of 4-5t/h is carried out every 8h when the specific gravity is less than 1.001. 5t/h closed cycle.
进一步,步骤8)中皮渣分离采用的方式为离心分离。Further, in step 8), the skin dregs is separated by centrifugation.
进一步,步骤9)中所述低温真空浓缩装置的转速为30rpm;温度条件为65℃。Further, the rotation speed of the low-temperature vacuum concentration device described in step 9) is 30 rpm; the temperature condition is 65°C.
本发明具有以下有益技术效果:The present invention has the following beneficial technical effects:
本发明使用国光、澳洲青苹和嘎啦三种苹果分别破碎后,按一定比例混合后压榨取汁,并与压榨果渣按比例混合后进行发酵,使发酵后的酒体具有适宜的香气和酸度。在原料处理步骤中用1:1的果汁和果渣混合,使苹果果皮中的香味能够很好地溶入苹果汁中。同时添加低温浓缩的压榨果汁和破碎后的未成熟青果与苹果皮肉混合进行低温浸渍发酵,提高了酒体单宁含量,显著提高了酒体厚重感,增强了酒体回味感。并在浸渍期添加复合酶显著提高了果皮中风味前体物质溶出率和分解,结合后期增香酵母的低温慢速浸渍发酵,显著增加了苹果酒中游离香气成分物质的含量,大大增加了苹果酒的果香。最后对部分成品酒进行蒸馏,将蒸馏酒再次加入成品酒中,提高了苹果果香气,解决了酒体风味差的问题。另因在工艺中加入抗氧化剂偏重亚硫酸钾和维生素C,并以二氧化碳保护,抑制了榨汁和发酵过程中的氧化褐变,保证了酒体澄清透明。离心沉淀方式防止酒液与氧气过多地接触,保证了酒体的稳定性。The invention uses three kinds of apples, Guoguang, Granny Smith and Gala, to be crushed respectively, mixed according to a certain ratio, squeezed to extract juice, and mixed with the squeezed pomace according to a ratio, and then fermented, so that the fermented wine body has a suitable aroma and acidity. In the raw material processing step, a 1:1 mixture of fruit juice and pomace is used, so that the aroma in the apple peel can be well dissolved in the apple juice. At the same time, low-temperature concentrated squeezed juice and crushed immature green fruit are mixed with apple skin for low-temperature maceration and fermentation, which increases the tannin content of the wine, significantly improves the thickness of the wine, and enhances the aftertaste of the wine. The addition of complex enzymes during the maceration period significantly improved the dissolution rate and decomposition of flavor precursors in the peel. Combined with the low-temperature and slow-speed maceration and fermentation of aroma-enhancing yeast in the later stage, the content of free aroma components in cider was significantly increased, and the flavor of apples was greatly increased. The fruity aroma of the wine. Finally, part of the finished wine is distilled, and the distilled wine is added to the finished wine again, which improves the apple fruit aroma and solves the problem of poor flavor of the wine. In addition, due to the addition of antioxidants potassium metabisulfite and vitamin C in the process, and the protection of carbon dioxide, the oxidative browning during the juicing and fermentation process is inhibited, and the wine body is clear and transparent. The centrifugal precipitation method prevents the wine from being in contact with oxygen too much and ensures the stability of the wine body.
附图说明Description of drawings
图1为本发明实施例的工艺流程图。Fig. 1 is the process flow diagram of the embodiment of the present invention.
具体实施方式detailed description
下面,参考附图,对本发明进行更全面的说明,附图中示出了本发明的示例性实施例。然而,本发明可以体现为多种不同形式,并不应理解为局限于这里叙述的示例性实施例。而是,提供这些实施例,从而使本发明全面和完整,并将本发明的范围完全地传达给本领域的普通技术人员。The present invention will now be described more fully with reference to the accompanying drawings, in which exemplary embodiments of the invention are shown. This invention may, however, be embodied in many different forms and should not be construed as limited to the exemplary embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art.
如图1所示,本发明提供了一种苹果混汁发酵酿造干型发酵酒的方法,该方法包括如下步骤:As shown in Figure 1, the present invention provides a kind of method for fermenting dry-type fermented wine with apple mixed juice, and the method comprises the steps:
(1)原料处理:将成熟的国光、澳洲青苹和嘎啦三种苹果,清洗干净后,用冷风吹干表面水份,使用栏式压榨机进行破碎,三种苹果分别破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例混合压榨得苹果清汁,之后将此混汁和果渣按1:1比例进行混合得混汁,在破碎的过程中按50-70mg/L的比例加入用苹果汁溶解的偏重亚硫酸钾以及20-40mg/L的Vc,同时用二氧化碳(每千升混汁加入0.5公斤)进行保护。(1) Raw material processing: After cleaning the mature Guoguang, Granny Smith and Gala apples, dry the surface moisture with cold wind, and crush them with a column press. The three kinds of apples are crushed according to Guoguang 70 %, Granny Smith apple 15%, and Gala apple 15% are mixed and squeezed to obtain apple juice, and then the mixed juice and pomace are mixed at a ratio of 1:1 to obtain a mixed juice. During the crushing process, 50-70mg/ Add potassium metabisulfite dissolved in apple juice and Vc of 20-40 mg/L in proportion to L, and protect with carbon dioxide (0.5 kg per thousand liters of mixed juice).
Vc与偏重亚硫酸钾同时加入,在防止混汁氧化的同时防止其受到杂菌的污染,且Vc的加入减少了偏重亚硫酸钾的用量,以此来保护苹果酒的特殊风味;二氧化碳的充入大大抑制了酒体的氧化褐变,起到最佳的保护作用。混汁和果渣按1:1比例进行混合,确保了酒体香气与口感的平衡。Vc and potassium metabisulfite are added at the same time to prevent the mixed juice from being polluted by bacteria while preventing oxidation, and the addition of Vc reduces the amount of potassium metabisulfite to protect the special flavor of cider; The addition greatly inhibits the oxidative browning of the wine body and plays the best protective role. The mixed juice and pomace are mixed at a ratio of 1:1 to ensure the balance between the aroma and taste of the wine.
(2)低温浓缩果汁:使用低温真空浓缩装置,按浓缩比例5:1,转速30rpm,在65℃条件下,对三种苹果混汁进行浓缩处理。也可采取低温冷冻捞冰的方式进行浓缩。(2) Low-temperature concentrated fruit juice: Use a low-temperature vacuum concentration device to concentrate the three kinds of apple mixed juices at a concentration ratio of 5:1 and a rotation speed of 30 rpm at 65°C. Concentration can also be carried out by means of low-temperature freezing and ice-fishing.
使用较低温度进行果汁浓缩,可以防止果汁变质,最大程度的保留果汁原有风味。Concentrating the juice at a lower temperature can prevent the juice from deteriorating and retain the original flavor of the juice to the greatest extent.
(3)带皮浸渍:在混汁中加入低温浓缩果汁(混汁与浓缩汁的添加比例为6:1-5:1)和经破碎的未成熟青果(果龄确定在8月份,落果收集后冷冻,加入量为100g/L混汁)。之后加入活化的β-葡萄糖苷酶、纤维素酶和增香果胶酶(1:2:2)(加入量为50mg/L混汁),在二氧化碳保护的条件下(加入量为0.5kg/千升混汁),在3-8℃下低温浸渍36-48h。(3) Dipping with skin: add low-temperature concentrated juice to the mixed juice (the ratio of mixed juice to concentrated juice is 6:1-5:1) and crushed immature green fruit (the fruit age is determined in August, and the fallen fruit is collected After freezing, the addition amount is 100g/L mixed juice). Then add activated β-glucosidase, cellulase and pectinase (1:2:2) (addition amount is 50mg/L mixed juice), under the condition of carbon dioxide protection (addition amount is 0.5kg/ 1,000 liters of mixed juice), dipped at 3-8°C for 36-48h at low temperature.
加入低温浓缩汁进行发酵能够提高酒体果香气,增强酒体风味特色。未成熟的青果果龄确定在8月份落果收集,这时果体中含有大量单宁,加入后进行发酵,能够提高酒体单宁含量,增强酒体厚重感与回味程度,解决了苹果酒口感寡淡的问题。纤维素酶和增香果胶酶的加入有效的提高了出汁率和果皮中风味前体物质的溶出率,β-葡萄糖苷酶的加入,大大增加了风味前体物质的分解,增加了苹果酒的果香。浸渍过程中保持低温浸渍,有效的防止了混汁中香味物质的流失。Adding low-temperature concentrated juice for fermentation can improve the fruity aroma of the wine body and enhance the flavor characteristics of the wine body. The age of immature green fruit is determined to be collected in August when the fruit falls. At this time, the fruit body contains a lot of tannin. After adding it for fermentation, it can increase the tannin content of the wine body, enhance the thickness and aftertaste of the wine body, and solve the problem of cider taste. Insipid question. The addition of cellulase and aroma-enhancing pectinase effectively improved the juice yield and the dissolution rate of flavor precursors in the peel, and the addition of β-glucosidase greatly increased the decomposition of flavor precursors and increased the yield of cider. fruity. Keeping the dipping at low temperature during the dipping process effectively prevents the loss of aroma substances in the mixed juice.
(4)成分调整:测定苹果混汁糖度,按照酿造12°苹果干酒的标准,加糖调整混汁的糖度至23%(蔗糖和果糖添加比例为2:1)。(4) Component adjustment: measure the sugar content of the apple juice mixture, according to the standard for brewing 12° apple dry wine, add sugar to adjust the sugar content of the mixture juice to 23% (the addition ratio of sucrose and fructose is 2:1).
在调整糖度的时候加入的是果糖和蔗糖,这样要大大增加了酒体的利口性,也避免了在发酵过程中杂醇的产生,以达到最佳的口感。When adjusting the sugar content, fructose and sucrose are added, which greatly increases the palatability of the wine and avoids the production of fusel alcohols during the fermentation process to achieve the best taste.
(5)增香酵母种子罐的建立:带皮浸渍24h后,取70-100L的混汁与小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16-18h。(5) Establishment of aroma-enhancing yeast seed tanks: After soaking the skins for 24 hours, take 70-100L of mixed juice and put it in a small tank for later use, and activate the dried aroma-enhancing yeast. Add juice; first, dissolve the dry aroma-enhancing yeast in pure water at 36-38°C at a ratio of 1:10, stir thoroughly, and activate for 15 minutes; take some apple juice and add it to the dissolved aroma-enhancing yeast in several times, while Measure the temperature of the activation solution while adding, take the temperature drop of the mixed juice at 5°C as the breaking point, continue activation for 15 minutes, stop adding the mixed juice when the temperature difference between the activation solution and the mixed juice is 3°C; mix the activation solution evenly into the 70- In the 100L mixed juice, an open circulation with a flow rate of 2t/h was performed immediately, and then the tank was closed and cultured at 28°C for 16-18h.
选用种子罐里的增香酵母,确保了增香酵母菌的快速繁殖和接种后的发酵均匀,缩短了发酵时间的同时维持了发酵罐内的稳定环境,同时降低了罐内局部温度积累,发酵后增强了酒体香气。The aroma-enhancing yeast in the seed tank is selected to ensure the rapid propagation of the aroma-enhancing yeast and the uniform fermentation after inoculation, shorten the fermentation time and maintain a stable environment in the fermenter, and reduce the local temperature accumulation in the tank. Afterwards, the aroma of the wine body is enhanced.
(6)接种增香酵母:按1:10的比例将种子罐中的混汁加入至发酵罐,之后立即进行一次流量为4t/h的开放式循环。(6) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h.
(7)发酵控制:发酵期间采用冷却装置保持发酵液温度为12-15℃,记录发酵液温度和比重。比重大于1.001时,每2-3h进行一次流量为4-5t/h的封闭式循环;比重小于1.001时,每8h进行一次流量为4-5t/h封闭式循环,直至终止发酵。比重降至为0.992时视为发酵终止。主发酵结束后,18-20℃浸渍24-48h。(7) Fermentation control: During the fermentation, a cooling device is used to keep the temperature of the fermentation broth at 12-15° C., and the temperature and specific gravity of the fermentation broth are recorded. When the specific gravity is greater than 1.001, a closed cycle with a flow rate of 4-5t/h is performed every 2-3 hours; when the specific gravity is less than 1.001, a closed cycle with a flow rate of 4-5t/h is performed every 8 hours until the fermentation is terminated. When the specific gravity drops to 0.992, the fermentation is considered to be terminated. After the main fermentation, soak for 24-48h at 18-20°C.
保持低温发酵会更好的浸提和保护苹果的芳香物质。封闭式循环的目的:苹果醪极其容易氧化,且氧化后其风味物质会发生改变,封闭式循环有效的减少了梨醪与氧气接触的时间,从而保护了苹果酒风味。Keeping the fermentation at low temperature will better extract and protect the aromatic substances of apples. The purpose of the closed cycle: the apple mash is extremely easy to oxidize, and its flavor substances will change after oxidation. The closed cycle effectively reduces the contact time of the pear mash with oxygen, thereby protecting the flavor of the cider.
(8)酒渣分离:浸渍结束后,反复进行皮渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(8) Separation of wine residues: After the immersion, the centrifugation of skin residues (5000rpm) is repeated until there are no large pieces of suspended matter in the separated original wine, and the centrifugation can be stopped.
离心机分离方式防止了酒液与氧气过多接触,也更好的保持了酒体风味物质。The centrifuge separation method prevents the wine from excessive contact with oxygen, and also better maintains the flavor substances of the wine body.
(9)蒸馏酒的处理与添加:使用低温真空浓缩装置,转速为30rpm、65℃条件下对部分苹果原酒进行蒸馏,取蒸馏酒酒身添加至苹果原酒中,蒸馏酒的添加量根据苹果酒最终酒度要求添加。(9) Treatment and addition of distilled wine: Use a low-temperature vacuum concentration device to distill part of the original apple wine with a rotation speed of 30 rpm and 65°C. Take the body of the distilled wine and add it to the original apple wine. The amount of distilled wine added is based on the The final alcohol level calls for addition.
低温蒸馏最大程度的保留了苹果酒中的风味物质成分。将蒸馏出的苹果酒再加入到发酵的苹果酒中,能够显著提高酒体风味成分,提高酒体香气,增强风味的同时还能够提高酒体回味程度。Low-temperature distillation preserves the flavor components in cider to the greatest extent. Adding the distilled cider to the fermented cider can significantly increase the flavor components of the wine body, improve the aroma of the wine body, enhance the flavor and improve the aftertaste of the wine body.
(10)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8d-10d形成沉渣,并去除。(10) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. 8d-10d after gluing treatment, sediment is formed and removed.
选用蛋清粉和硅藻土进行复合下胶处理,更好的保证了将来酒体的澄清性和稳定性。下胶处理过程中加入偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。Egg white powder and diatomaceous earth are selected for compound gumming treatment, which can better ensure the clarity and stability of the wine body in the future. Potassium metabisulfite and Vc are added during the gumming process to inhibit bacterial contamination and prevent browning.
(11)稳定性处理:对发酵罐进行降温处理,保持发酵罐内酒体温度为-4.5℃,维持10-14d。(11) Stability treatment: cooling the fermenter to keep the wine body temperature at -4.5°C for 10-14 days.
选择接近酒体冰点温度-4.5℃,并延长冷稳定处理的时间,加速了酒体中沉淀的下沉,防止风味物质的挥发与破坏,所以我们采用冷处理(长时间处理)的办法来除去酒中色素,蛋白质等沉淀,以达到稳定,降低酸涩味,圆润梨酒口感的目的。Choose a temperature close to the freezing point of the wine body -4.5°C, and prolong the time of cold stabilization treatment, which accelerates the sinking of sediment in the wine body and prevents the volatilization and destruction of flavor substances. Therefore, we use cold treatment (long time treatment) to remove alcohol. Pigment, protein, etc. are precipitated to achieve stability, reduce sourness and astringency, and round the mouthfeel of pear wine.
(12)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和菌类。之后经换桶、陈酿、罐装、包装成品等步骤完成苹果酒的制备。(12) Filtration: use cross-flow filtration to filter the wine body to remove sediment and fungi. Afterwards, the preparation of cider is completed through steps such as changing barrels, aging, canning, and packaging finished products.
采用错流过滤方式,在符合酒体出厂质量的同时,大大减缓了过滤的时间。The cross-flow filtration method is adopted, which greatly reduces the filtration time while meeting the factory quality of the wine body.
为进一步证明本发明的技术效果,申请人做了如下实验:In order to further prove the technical effect of the present invention, the applicant has done following experiments:
实验1,采用三种苹果混合压榨后的清汁发酵:In experiment 1, three kinds of apples were mixed and squeezed for clear juice fermentation:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨得苹果清汁;破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the ratio of 70% of Guoguang, 15% of Granny Smith, and 15% of Gala apples, they are mixed and squeezed to obtain clear apple juice; Add 0.5kg to 1,000 liters of mixed juice and fill it with carbon dioxide gas for protection.
(2)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,使其达到酿造12°苹果干酒的标准。(2) Sugar content adjustment: measure the sugar content of the apple juice mixture, wherein the ratio of sucrose to fructose is 2:1, adjust the sugar content of the mixed juice to 23%, so that it reaches the standard for brewing 12° apple dry wine.
(3)种子罐的建立:取70L的果汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L果汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取果汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以果汁温度下降5℃为断点,继续活化15min,当活化液与果汁的温度差为3℃时停止添加果汁;将活化液均匀混入前期准备的70-100L果汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,28℃条件下培养16-18h。(3) Establishment of the seed tank: take 70L of fruit juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800mg/L fruit juice; Dissolve in pure water at 36-38°C, fully stir, and activate for 15 minutes; take some fruit juice, add it to the dissolved aroma-enhancing yeast several times, measure the temperature of the activation solution while adding, and take the juice temperature to drop by 5°C as the cut-off point, continue to activate for 15 minutes, and stop adding juice when the temperature difference between the activation solution and the juice is 3°C; mix the activation solution evenly into the 70-100L juice prepared earlier, and immediately perform an open cycle with a flow rate of 2t/h, and then Close the tank and culture at 28°C for 16-18h.
(4)接种增香酵母:将种子罐中的果汁和发酵罐中的果汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(4) Inoculation of aroma-enhancing yeast: add the juice in the seed tank and the juice in the fermenter to the fermenter at a ratio of 1:10, and immediately carry out an open cycle with a flow rate of 4t/h
(5)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发酵液的比重大于1.030时,采用每2-3h进行一次流量为4-5t/h的封闭式循环;直至终止发酵。比重降至为0.992时视为发酵终止。主发酵结束后,18-20℃浸渍24-48h。(5) Fermentation control: During the fermentation, a cooling device is used to keep the temperature of the fermentation broth at about 13-15°C, and the temperature and specific gravity of the fermentation broth are recorded. When the specific gravity of the fermentation broth is greater than 1.030, the flow rate is 4-5t every 2-3h /h closed cycle; until the fermentation is terminated. When the specific gravity drops to 0.992, the fermentation is considered to be terminated. After the main fermentation, soak for 24-48h at 18-20°C.
(6)酒渣分离:浸渍结束后,反复进行酒渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(6) Separation of wine residues: after the immersion, centrifugation of wine residues (5000rpm) is carried out repeatedly, and the centrifugation can be stopped when there is no large suspended matter in the separated original wine.
(7)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8-10d形成沉渣,并去除。(7) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. After 8-10 days of gluing treatment, sediment is formed and removed.
(8)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(8) Stability treatment: after the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(9)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(9) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,申请人发现,使用国光、澳洲青苹和嘎啦三种苹果分别破碎后,按比例混合并压榨取汁后进行发酵,使发酵后的酒体具有适宜的香气和酸度。During the production process, the applicant found that three kinds of apples, namely Guoguang, Granny Smith and Gala, were used to be crushed, mixed in proportion, squeezed to obtain juice and then fermented, so that the fermented wine body had suitable aroma and acidity.
实验2,采用三种苹果混合压榨后的清汁+果渣发酵的方式:In experiment 2, three kinds of apples were mixed and squeezed with clear juice + pomace fermentation:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨,收集果汁和果渣,并按1:1(kg/L)混合得混汁,破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the proportion of Guoguang 70%, Granny Smith 15%, and Gala 15%, mix and squeeze, collect the juice and pomace, and mix it at 1:1 (kg/L) to get a mixed juice. Potassium sulfate (50mg/L) and VC (20mg/L), while adding 0.5kg per thousand liters of mixed juice to fill with carbon dioxide gas for protection.
(2)带皮浸渍:在混汁中按50mg/L的比例加入活化好的复合酶(β-葡萄糖苷酶、纤维素酶和增香果胶酶,比例为1:2:2),在二氧化碳保护的条件下(每千升混汁充入0.5kgCO2气体)3-8℃浸渍42h。(2) Dipping with skin: Add activated compound enzyme (β-glucosidase, cellulase and flavoring pectinase in a ratio of 1:2:2) to the mixed juice at a ratio of 50 mg/L. Under the condition of carbon dioxide protection (0.5kg CO2 gas per thousand liters of mixed juice) immerse at 3-8°C for 42h.
(3)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,使其达到酿造12°苹果干酒的标准。(3) Sugar content adjustment: measure the sugar content of apple mixed juice, wherein the ratio of sucrose and fructose is 2:1, adjust the sugar content of mixed juice to 23%, so that it reaches the standard for brewing 12° apple dry wine.
(4)种子罐的建立:在带皮浸渍24h后,取70L的混汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,28℃条件下培养16-18h。(4) Establishment of the seed tank: After soaking the skin for 24 hours, take 70L of the mixed juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800 mg/L of the mixed juice; first Dissolve dry aromatizing yeast in pure water at 36-38°C at a ratio of 1:10, stir thoroughly, and activate for 15 minutes; take some apple juice and add it to the dissolved aromatizing yeast several times, and measure the activation while adding Solution temperature, with the temperature of the mixed juice dropping 5°C as the breaking point, continue to activate for 15 minutes, stop adding the mixed juice when the temperature difference between the activated solution and the mixed juice is 3°C; mix the activated solution evenly into the 70-100L mixed juice prepared earlier , Immediately perform an open cycle with a flow rate of 2t/h, then close the tank, and cultivate at 28°C for 16-18h.
(5)接种增香酵母:将种子罐中的混汁和发酵罐中的混汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(5) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank and the mixed juice in the fermenter to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h
(6)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发酵液的比重大于1.030时,采用每2-3h进行一次流量为4-5t/h的封闭式循环;直至终止发酵。比重降至为0.992时视为发酵终止。主发酵结束后,18℃-20℃浸渍24-48h。(6) Fermentation control: During fermentation, use a cooling device to keep the temperature of the fermentation broth at about 13-15°C, record the temperature and specific gravity of the fermentation broth, and when the specific gravity of the fermentation broth is greater than 1.030, use a flow rate of 4-5t every 2-3h /h closed cycle; until the fermentation is terminated. When the specific gravity drops to 0.992, the fermentation is considered to be terminated. After the main fermentation, soak for 24-48h at 18°C-20°C.
(7)酒渣分离:浸渍结束后,反复进行皮渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(7) Separation of wine residues: After the immersion, the centrifugation of skin residues (5000rpm) is repeated until there is no large suspended matter in the separated original wine, and the centrifugation can be stopped.
(8)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8-10d形成沉渣,并去除。(8) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. After 8-10 days of gluing treatment, sediment is formed and removed.
(9)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(9) Stability treatment: after the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(10)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(10) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,申请人发现,使用国光、澳洲青苹和嘎啦三种苹果进行混合发酵,使发酵后的酒体具有适宜的香气和酸度。通过在混汁中添加复合酶进行浸渍发酵,显著提高了果皮中风味前体物质的溶出率和分解,大大提高了苹果酒的果香。另外混汁发酵时果汁与果渣的比例对于保持后续工序过程中苹果酒的香味也起到至关重要的作用。因为果渣含量过高会增加酒体的苦涩感,且不利于后期的澄清处理,而果渣含量较低则会使酒体风味寡淡香气不突出。During the production process, the applicant found that three kinds of apples, Guoguang, Granny Smith and Gala, were used for mixed fermentation, so that the fermented wine body had suitable aroma and acidity. Adding complex enzymes to the mixed juice for immersion fermentation significantly improved the dissolution rate and decomposition of flavor precursors in the peel, and greatly improved the fruity aroma of the cider. In addition, the ratio of juice to pomace during mixed juice fermentation also plays a vital role in maintaining the aroma of cider in the subsequent process. Because too much pomace content will increase the bitterness of the wine body, and it is not conducive to the later clarification treatment, while the low content of pomace will make the wine body less flavorful and less prominent.
实验3,采用三种苹果混合压榨后的清汁+果渣得混汁,并添加青果果肉和低温浓缩汁发酵的方式,即本发明所采用的方法:In experiment 3, three kinds of apples were mixed and squeezed to obtain mixed juice with clear juice+pomace, and the method of adding green fruit pulp and low-temperature concentrated juice to ferment was the method adopted in the present invention:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨,收集果汁和果渣,并按1:1(kg/L)混合得混汁,破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the proportion of Guoguang 70%, Granny Smith 15%, and Gala 15%, mix and squeeze, collect the juice and pomace, and mix it at 1:1 (kg/L) to get a mixed juice. Potassium sulfate (50mg/L) and VC (20mg/L), while adding 0.5kg per thousand liters of mixed juice to fill with carbon dioxide gas for protection.
(2)低温浓缩果汁:使用低温真空浓缩装置,按浓缩比例5:1,转速30rpm,在65℃条件下,对三种苹果混汁进行浓缩处理。也可采取低温冷冻捞冰的方式进行浓缩。(2) Low-temperature concentrated fruit juice: Use a low-temperature vacuum concentration device to concentrate the three kinds of apple mixed juices at a concentration ratio of 5:1 and a rotation speed of 30 rpm at 65°C. Concentration can also be carried out by means of low-temperature freezing and ice-fishing.
(3)带皮浸渍:在混汁中加入低温浓缩果汁(混汁与浓缩汁比例为6:1-5:1)和经破碎的未成熟青果(果龄确定在8月份,落果收集后冷冻,加入量为100g/L混汁)。之后加入活化的β-葡萄糖苷酶、纤维素酶和增香果胶酶(1:2:2)(加入量为50mg/L混汁),在二氧化碳保护的条件下(加入量为0.5kg/千升混汁),在3-8℃下低温浸渍36-48h。(3) Dipping with skin: add low-temperature concentrated fruit juice (the ratio of mixed juice to concentrated juice is 6:1-5:1) and crushed immature green fruit (the fruit age is determined in August, and freeze after the fruit drop is collected) , the addition amount is 100g/L mixed juice). Then add activated β-glucosidase, cellulase and pectinase (1:2:2) (addition amount is 50mg/L mixed juice), under the condition of carbon dioxide protection (addition amount is 0.5kg/ 1,000 liters of mixed juice), dipped at 3-8°C for 36-48h at low temperature.
(4)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,以使其达到酿造12°苹果干酒的标准。(4) Adjustment of sugar content: measure the sugar content of the apple mixed juice, wherein the ratio of sucrose to fructose is 2:1, adjust the sugar content of the mixed juice to 23%, so that it can reach the standard for brewing 12° apple dry wine.
(5)种子罐的建立:在带皮浸渍24h后,取70L的混汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16-18h。(5) Establishment of the seed tank: after immersing the skins for 24 hours, take 70L of the mixed juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800 mg/L of the mixed juice; first Dissolve dry aromatizing yeast in pure water at 36-38°C at a ratio of 1:10, stir thoroughly, and activate for 15 minutes; take some apple juice and add it to the dissolved aromatizing yeast several times, and measure the activation while adding Solution temperature, with the temperature of the mixed juice dropping 5°C as the breaking point, continue to activate for 15 minutes, stop adding the mixed juice when the temperature difference between the activated solution and the mixed juice is 3°C; mix the activated solution evenly into the 70-100L mixed juice prepared earlier , Immediately perform an open cycle with a flow rate of 2t/h, then close the tank, and cultivate at 28°C for 16-18h.
(6)接种增香酵母:将种子罐中的混汁和发酵罐中的混汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(6) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank and the mixed juice in the fermenter to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h
(7)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发现发酵液的比重为N,大于1.030,采用每2-3h进行一次流量为4-5t/h的封闭式循环;(7) Fermentation control: During the fermentation, a cooling device is used to keep the temperature of the fermented liquid at about 13-15°C, and the temperature and specific gravity of the fermented liquid are recorded. It is found that the specific gravity of the fermented liquid is N, greater than 1.030, and the flow rate is carried out every 2-3h. 4-5t/h closed cycle;
(8)酒渣分离:浸渍结束后,反复进行皮渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(8) Separation of wine residues: After the immersion, the centrifugation of skin residues (5000rpm) is repeated until there are no large pieces of suspended matter in the separated original wine, and the centrifugation can be stopped.
(9)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8d-10d形成沉渣,并去除。(9) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. 8d-10d after gluing treatment, sediment is formed and removed.
(10)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(10) Stability treatment: After the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(11)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(11) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,发明人发现,使用国光、澳洲青苹和嘎啦三种苹果进行混合发酵,使发酵后的酒体具有适宜的香气和酸度。通过在混汁中添加复合酶进行浸渍发酵,显著提高了果皮中风味前体物质的溶出率和分解,大大提高了苹果酒的果香。对苹果汁进行浓缩时,也可采取低温冷冻后捞冰的方式进行浓缩。将破碎后的未成熟青果与苹果皮肉混合进行发酵,提高了酒体单宁含量,显著增强了酒体厚重感和回味感。另外混汁发酵时果汁与果渣的比例对于保持后续工序过程中苹果酒的香味也起到至关重要的作用。因为果渣含量过高会增加酒体的苦涩感,且不利于后期的澄清处理,而果渣含量较低则会使酒体风味寡淡香气不突出。During the production process, the inventor found that three kinds of apples, Guoguang, Granny Smith and Gala, were used for mixed fermentation, so that the fermented wine body had suitable aroma and acidity. Adding complex enzymes to the mixed juice for immersion fermentation significantly improved the dissolution rate and decomposition of flavor precursors in the peel, and greatly improved the fruity aroma of the cider. When the apple juice is concentrated, it can also be concentrated by freezing it at a low temperature and then scooping up ice. The crushed immature green fruit is mixed with apple skin for fermentation, which increases the tannin content of the wine body, and significantly enhances the body and aftertaste of the wine. In addition, the ratio of juice to pomace during mixed juice fermentation also plays a vital role in maintaining the aroma of cider in the subsequent process. Because too much pomace content will increase the bitterness of the wine body, and it is not conducive to the later clarification treatment, while the low content of pomace will make the wine body less flavorful and less prominent.
实验4,采用三种苹果混合压榨后的清汁发酵原酒+蒸馏酒的方式:In experiment 4, three kinds of apples were mixed and squeezed to ferment the original wine + distilled wine:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨并收集果汁用于发酵,破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the ratio of 70% of Guoguang, 15% of Granny Smith and 15% of Gala apple, mix and squeeze and collect the juice for fermentation. Add potassium metabisulfite (50mg/L) and VC (20mg/L) during the crushing process, At the same time, add 0.5kg per thousand liters of mixed juice and fill it with carbon dioxide gas for protection.
(2)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,使其达到酿造12°苹果干酒的标准。(2) Sugar content adjustment: measure the sugar content of the apple juice mixture, wherein the ratio of sucrose to fructose is 2:1, adjust the sugar content of the mixed juice to 23%, so that it reaches the standard for brewing 12° apple dry wine.
(3)种子罐的建立:取70L的果汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L果汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取果汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以果汁温度下降5℃为断点,继续活化15min,当活化液与果汁的温度差为3℃时停止添加果汁;将活化液均匀混入前期准备的70-100L果汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16~18h。(3) Establishment of the seed tank: take 70L of fruit juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800mg/L fruit juice; Dissolve in pure water at 36-38°C, fully stir, and activate for 15 minutes; take some fruit juice, add it to the dissolved aroma-enhancing yeast several times, measure the temperature of the activation solution while adding, and take the juice temperature to drop by 5°C as the cut-off point, continue to activate for 15 minutes, and stop adding juice when the temperature difference between the activation solution and the juice is 3°C; mix the activation solution evenly into the 70-100L juice prepared earlier, and immediately perform an open cycle with a flow rate of 2t/h, and then Close the tank and incubate at 28°C for 16-18 hours.
(4)接种增香酵母:将种子罐中的混汁和发酵罐中的混汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(4) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank and the mixed juice in the fermenter to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h
(5)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发现发酵液的比重为N,大于1.030,采用每2-3h进行一次流量为4-5t/h的封闭式循环;(5) Fermentation control: During the fermentation period, a cooling device is used to keep the temperature of the fermented liquid at about 13-15°C, and the temperature and specific gravity of the fermented liquid are recorded. It is found that the specific gravity of the fermented liquid is N, which is greater than 1.030. 4-5t/h closed cycle;
(6)酒渣分离:浸渍结束后,反复进行酒渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(6) Separation of wine residues: after the immersion, centrifugation of wine residues (5000rpm) is carried out repeatedly, and the centrifugation can be stopped when there is no large suspended matter in the separated original wine.
(7)蒸馏酒的处理与添加:使用生产设备-低温真空浓缩装置,65℃转速30rpm,对已得苹果发酵酒进行蒸馏,之后将所得蒸馏酒添加到发酵所得苹果原酒中,蒸馏酒的添加量根据苹果酒最终酒度要求添加。(7) Treatment and addition of distilled wine: Use the production equipment - low temperature vacuum concentration device, 65 ° C and 30 rpm to distill the obtained apple fermented wine, and then add the obtained distilled wine to the fermented apple original wine, the addition of distilled wine The amount is added according to the final alcohol content of the cider.
(8)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8-10d形成沉渣,并去除。(8) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. After 8-10 days of gluing treatment, sediment is formed and removed.
(9)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(9) Stability treatment: after the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(10)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(10) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,发明人发现,使用国光、澳洲青苹和嘎啦三种苹果分别破碎,按比例压榨后的苹果汁进行发酵,使发酵后的酒体具有适宜的香气和酸度。之后在所得原酒中添加原酒的蒸馏酒,显著提高了苹果酒的果香。During the production process, the inventor found that three kinds of apples, namely Guoguang, Granny Smith and Gala, were used to be crushed respectively, and the apple juice squeezed in proportion was used for fermentation, so that the fermented wine body had suitable aroma and acidity. Afterwards, the distilled spirit of the original wine is added to the obtained original wine, which significantly improves the fruity aroma of the cider.
实验5,采用三种苹果混合压榨后的清汁+果渣发酵原酒+蒸馏酒的方式:In experiment 5, three kinds of apples were mixed with squeezed clear juice + pomace fermented original wine + distilled wine:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨,收集果汁和果渣,并按1:1(kg/L)混合得混汁,破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the proportion of Guoguang 70%, Granny Smith 15%, and Gala 15%, mix and squeeze, collect the juice and pomace, and mix it at 1:1 (kg/L) to get a mixed juice. Potassium sulfate (50mg/L) and VC (20mg/L), while adding 0.5kg per thousand liters of mixed juice to fill with carbon dioxide gas for protection.
(2)带皮浸渍:在混汁中按50mg/L的比例加入活化好的复合酶(β-葡萄糖苷酶、纤维素酶和增香果胶酶,比例为1:2:2),在二氧化碳保护的条件下(每千升混汁充入0.5kgCO2气体)3-8℃浸渍42h。(2) Dipping with skin: Add activated compound enzyme (β-glucosidase, cellulase and flavoring pectinase in a ratio of 1:2:2) to the mixed juice at a ratio of 50 mg/L. Under the condition of carbon dioxide protection (0.5kg CO2 gas per thousand liters of mixed juice) immerse at 3-8°C for 42h.
(3)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,使其达到酿造12°苹果干酒的标准。(3) Sugar content adjustment: measure the sugar content of apple mixed juice, wherein the ratio of sucrose and fructose is 2:1, adjust the sugar content of mixed juice to 23%, so that it reaches the standard for brewing 12° apple dry wine.
(4)种子罐的建立:在带皮浸渍24h后,取70L的混汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16-18h。(4) Establishment of the seed tank: After soaking the skin for 24 hours, take 70L of the mixed juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800 mg/L of the mixed juice; first Dissolve dry aromatizing yeast in pure water at 36-38°C at a ratio of 1:10, stir thoroughly, and activate for 15 minutes; take some apple juice and add it to the dissolved aromatizing yeast several times, and measure the activation while adding Solution temperature, with the temperature of the mixed juice dropping 5°C as the breaking point, continue to activate for 15 minutes, stop adding the mixed juice when the temperature difference between the activated solution and the mixed juice is 3°C; mix the activated solution evenly into the 70-100L mixed juice prepared earlier , Immediately perform an open cycle with a flow rate of 2t/h, then close the tank, and cultivate at 28°C for 16-18h.
(5)接种增香酵母:将种子罐中的混汁和发酵罐中的混汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(5) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank and the mixed juice in the fermenter to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h
(6)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发酵液的比重大于1.030时,采用每2-3h进行一次流量为4-5t/h的封闭式循环;直至终止发酵。比重降至为0.992时视为发酵终止。主发酵结束后,18-20℃浸渍24-48h。(6) Fermentation control: During fermentation, use a cooling device to keep the temperature of the fermentation broth at about 13-15°C, record the temperature and specific gravity of the fermentation broth, and when the specific gravity of the fermentation broth is greater than 1.030, use a flow rate of 4-5t every 2-3h /h closed cycle; until the fermentation is terminated. When the specific gravity drops to 0.992, the fermentation is considered to be terminated. After the main fermentation, soak for 24-48h at 18-20°C.
(7)酒渣分离:浸渍结束后,反复进行皮渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(7) Separation of wine residues: After the immersion, the centrifugation of skin residues (5000rpm) is repeated until there is no large suspended matter in the separated original wine, and the centrifugation can be stopped.
(8)蒸馏酒的处理与添加:使用生产设备-低温真空浓缩装置,65℃转速30rpm,对已得苹果发酵酒进行蒸馏,之后将所得蒸馏酒添加到发酵所得苹果原酒中,蒸馏酒的添加量根据苹果酒最终酒度要求添加。(8) Treatment and addition of distilled wine: use production equipment-low temperature vacuum concentration device, 65°C and 30rpm to distill the obtained apple fermented wine, and then add the obtained distilled wine to the fermented apple original wine, the addition of distilled wine The amount is added according to the final alcohol content of the cider.
(9)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8-10d形成沉渣,并去除。(9) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. After 8-10 days of gluing treatment, sediment is formed and removed.
(10)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(10) Stability treatment: After the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(11)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(11) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,发明人发现,使用国光、澳洲青苹和嘎啦三种苹果进行混合发酵,使发酵后的酒体具有适宜的香气和酸度。通过在混汁中添加复合酶进行浸渍发酵,显著提高了果皮中风味前体物质的溶出率和分解,大大提高了苹果酒的果香。之后在所得原酒中添加原酒的蒸馏酒,也显著提高了苹果酒的果香。另外混汁发酵时果汁与果渣的比例对于保持后续工序过程中苹果酒的香味也起到至关重要的作用。因为果渣含量过高会增加酒体的苦涩感,且不利于后期的澄清处理,而果渣含量较低则会使酒体风味寡淡香气不突出。During the production process, the inventor found that three kinds of apples, Guoguang, Granny Smith and Gala, were used for mixed fermentation, so that the fermented wine body had suitable aroma and acidity. Adding complex enzymes to the mixed juice for immersion fermentation significantly improved the dissolution rate and decomposition of flavor precursor substances in the peel, and greatly improved the fruity aroma of cider. Afterwards, the distilled liquor of the original wine is added to the obtained original wine, which also significantly improves the fruity aroma of the cider. In addition, the ratio of juice to pomace during mixed juice fermentation also plays a vital role in maintaining the aroma of cider in the subsequent process. Because too much pomace content will increase the bitterness of the wine body, and it is not conducive to the later clarification treatment, while the low content of pomace will make the wine body less flavorful and less prominent.
实验6,采用三种苹果混合压榨后的清汁+果渣+浓缩汁和青果发酵原酒+蒸馏酒的方式:In experiment 6, three kinds of apples were mixed and squeezed clear juice + pomace + concentrated juice and green fruit fermented original wine + distilled wine:
(1)选取成熟且存放一段时间后的国光、澳洲青苹、嘎啦苹果共(1000)斤,清洗干净,用冷风吹干表面水分,三种苹果分别用栏式压榨机进行破碎,破碎后按照国光70%、澳洲青苹15%、嘎啦苹果15%的比例进行混合并压榨,收集果汁和果渣,并按1:1(kg/L)混合得混汁,破碎过程中加入偏重亚硫酸钾(50mg/L)和VC(20mg/L),同时按每千升混汁加入0.5kg的量充入二氧化碳气体进行保护。(1) Choose Guoguang, Granny Smith, and Gala apples (1000 catties) that are mature and stored for a period of time, clean them, and dry the surface moisture with cold air. According to the proportion of Guoguang 70%, Granny Smith 15%, and Gala 15%, mix and squeeze, collect the juice and pomace, and mix it at 1:1 (kg/L) to get a mixed juice. Potassium sulfate (50mg/L) and VC (20mg/L), while adding 0.5kg per thousand liters of mixed juice to fill with carbon dioxide gas for protection.
(2)低温浓缩果汁:使用低温真空浓缩装置,按浓缩比例5:1,转速30rpm,在65℃条件下,对三种苹果混汁进行浓缩处理。也可采取低温冷冻捞冰的方式进行浓缩。(2) Low-temperature concentrated fruit juice: Use a low-temperature vacuum concentration device to concentrate the three kinds of apple mixed juices at a concentration ratio of 5:1 and a rotation speed of 30 rpm at 65°C. Concentration can also be carried out by means of low-temperature freezing and ice-fishing.
(3)带皮浸渍:在混汁中加入低温浓缩果汁(混汁与浓缩汁比例为6:1-5:1)和经破碎的未成熟青果(果龄确定在8月份,落果收集后冷冻,加入量为100g/L混汁)。之后加入活化的β-葡萄糖苷酶、纤维素酶和增香果胶酶(1:2:2)(加入量为50mg/L混汁),在二氧化碳保护的条件下(加入量为0.5kg/千升混汁),在3-8℃下低温浸渍36-48h。(3) Dipping with skin: add low-temperature concentrated fruit juice (the ratio of mixed juice to concentrated juice is 6:1-5:1) and crushed immature green fruit (the fruit age is determined in August, and freeze after the fruit drop is collected) , the addition amount is 100g/L mixed juice). Then add activated β-glucosidase, cellulase and pectinase (1:2:2) (addition amount is 50mg/L mixed juice), under the condition of carbon dioxide protection (addition amount is 0.5kg/ 1,000 liters of mixed juice), dipped at 3-8°C for 36-48h at low temperature.
(4)糖度调整:测出苹果混汁的糖度,其中蔗糖和果糖的比例为2:1,调整混汁的糖度到23%,以使其达到酿造12°苹果干酒的标准。(4) Adjustment of sugar content: measure the sugar content of the apple mixed juice, wherein the ratio of sucrose to fructose is 2:1, adjust the sugar content of the mixed juice to 23%, so that it can reach the standard for brewing 12° apple dry wine.
(5)种子罐的建立:在带皮浸渍24h后,取70L的混汁于小罐中备用,将活化干增香酵母进行活化,活化方法为:按600-800mg/L混汁添加;首先将干增香酵母按1:10的比例溶解于36-38℃纯水中,充分搅拌,活化15min;取苹果混汁若干,分多次添加至溶解的增香酵母中,边添加边测量活化液温度,以混汁温度下降5℃为断点,继续活化15min,当活化液与混汁的温度差为3℃时停止添加混汁;将活化液均匀混入前期准备的70-100L混汁中,立即进行一次流量为2t/h的开放式循环,然后闭罐,在28℃条件下培养16-18h。(5) Establishment of the seed tank: after immersing the skins for 24 hours, take 70L of the mixed juice in a small tank for later use, and activate the dried aroma-enhancing yeast. The activation method is: add 600-800 mg/L of the mixed juice; first Dissolve dry aromatizing yeast in pure water at 36-38°C at a ratio of 1:10, stir thoroughly, and activate for 15 minutes; take some apple juice and add it to the dissolved aromatizing yeast several times, and measure the activation while adding Solution temperature, with the temperature of the mixed juice dropping 5°C as the breaking point, continue to activate for 15 minutes, stop adding the mixed juice when the temperature difference between the activated solution and the mixed juice is 3°C; mix the activated solution evenly into the 70-100L mixed juice prepared earlier , Immediately perform an open cycle with a flow rate of 2t/h, then close the tank, and cultivate at 28°C for 16-18h.
(6)接种增香酵母:将种子罐中的混汁和发酵罐中的混汁按1:10的比例加入到发酵罐中,加完立即进行一次流量为4t/h的开放式循环(6) Inoculation of aroma-enhancing yeast: Add the mixed juice in the seed tank and the mixed juice in the fermenter to the fermenter at a ratio of 1:10, and immediately perform an open cycle with a flow rate of 4t/h
(7)发酵控制:发酵期间采用冷却装置保持发酵液温度在13-15℃左右,记录发酵液的温度和比重,发现发酵液的比重为N,大于1.030,采用每2-3h进行一次流量为4-5t/h的封闭式循环;(7) Fermentation control: During the fermentation, a cooling device is used to keep the temperature of the fermented liquid at about 13-15°C, and the temperature and specific gravity of the fermented liquid are recorded. It is found that the specific gravity of the fermented liquid is N, greater than 1.030, and the flow rate is carried out every 2-3h. 4-5t/h closed cycle;
(8)酒渣分离:浸渍结束后,反复进行皮渣离心分离(5000rpm),至分离到的原酒中无大块悬浮物时即可停止离心。(8) Separation of wine residues: After the immersion, the centrifugation of skin residues (5000rpm) is repeated until there are no large pieces of suspended matter in the separated original wine, and the centrifugation can be stopped.
(9)蒸馏酒的处理与添加:使用生产设备-低温真空浓缩装置,65℃转速30rpm,对已得苹果发酵酒进行蒸馏,之后将所得蒸馏酒添加到发酵所得苹果原酒中,蒸馏酒的添加量根据苹果酒最终酒度要求添加。(9) Treatment and addition of distilled wine: use the production equipment - low temperature vacuum concentration device, 65 ° C and 30 rpm to distill the obtained apple fermented wine, and then add the obtained distilled wine to the fermented apple original wine, the addition of distilled wine The amount is added according to the final alcohol content of the cider.
(10)下胶处理:按0.3-0.5g/L的用量加入皂土和0.05g/L的蛋清粉,与酒体充分混合。在此期间分别加入20g/t偏重亚硫酸钾和Vc来抑制细菌污染和防止褐变。下胶处理后8-10d形成沉渣,并去除。(10) Gluing treatment: add bentonite and 0.05g/L egg white powder in an amount of 0.3-0.5g/L, and fully mix with the wine body. During this period, 20g/t potassium metabisulfite and Vc were added respectively to inhibit bacterial contamination and prevent browning. After 8-10 days of gluing treatment, sediment is formed and removed.
(11)稳定性处理:下胶去除沉渣后对发酵罐进行降温处理,保持罐内酒体温度为-4.5℃,维持10-14d。(11) Stability treatment: After the glue is removed to remove the sediment, the temperature of the fermenter is lowered, and the temperature of the wine body in the tank is kept at -4.5°C for 10-14 days.
(12)过滤:采用错流过滤对酒体进行过滤,除去沉淀物质和微生物。(12) Filtration: use cross-flow filtration to filter the wine body to remove sediment and microorganisms.
在生产过程中,申请人发现,使用国光、澳洲青苹和嘎啦三种苹果进行混合发酵,使发酵后的酒体具有适宜的香气和酸度。通过在混汁中添加复合酶进行浸渍发酵,显著提高了果皮中风味前体物质的溶出率和分解,大大提高了苹果酒的果香。对苹果汁进行浓缩时,也可采取低温冷冻后捞冰的方式进行浓缩。将破碎后的未成熟青果与苹果皮肉混合进行发酵,提高了酒体单宁含量,显著增强了酒体厚重感和回味感。之后在所得原酒中添加原酒的蒸馏酒,也显著提高了苹果酒的果香。另外混汁发酵时果汁与果渣的比例对于保持后续工序过程中苹果酒的香味也起到至关重要的作用。因为果渣含量过高会增加酒体的苦涩感,且不利于后期的澄清处理,而果渣含量较低则会使酒体风味寡淡香气不突出。During the production process, the applicant found that three kinds of apples, Guoguang, Granny Smith and Gala, were used for mixed fermentation, so that the fermented wine body had suitable aroma and acidity. Adding complex enzymes to the mixed juice for immersion fermentation significantly improved the dissolution rate and decomposition of flavor precursor substances in the peel, and greatly improved the fruity aroma of cider. When the apple juice is concentrated, it can also be concentrated by freezing it at a low temperature and then scooping up ice. The crushed immature green fruit is mixed with apple skin for fermentation, which increases the tannin content of the wine body, and significantly enhances the body and aftertaste of the wine. Afterwards, the distilled liquor of the original wine is added to the obtained original wine, which also significantly improves the fruity aroma of the cider. In addition, the ratio of juice to pomace during mixed juice fermentation also plays a vital role in maintaining the aroma of cider in the subsequent process. Because too much pomace content will increase the bitterness of the wine body, and it is not conducive to the later clarification treatment, while the low content of pomace will make the wine body less flavorful and less prominent.
申请人注意到,在工艺中使用二氧化碳保护对防止酒体的褐变起着非常重要的作用。另外,离心分离可以防止酒液与氧气过多接触,既保持了酒体的风味,也防止了酒体褐变。另外,在此强调的是,发明人选择接近酒体冰点温度-4.5℃,并延长冷稳定处理的时间,加速了酒体中沉淀的下沉,防止风味物质的挥发与破坏,所以我们采用冷处理(长时间处理)的办法来除去酒中色素,蛋白质等沉淀,以达到稳定,降低酸涩味,圆润苹果酒口感的目的。The applicant noticed that the use of carbon dioxide protection in the process plays a very important role in preventing the browning of the wine body. In addition, centrifugation can prevent the wine from excessive contact with oxygen, which not only maintains the flavor of the wine, but also prevents the wine from browning. In addition, what is emphasized here is that the inventor chose a temperature close to the freezing point of the wine body -4.5°C, and extended the time of cold stabilization treatment, which accelerated the sinking of the sediment in the wine body and prevented the volatilization and destruction of flavor substances, so we adopted cold treatment (Long time treatment) method to remove the pigment, protein and other precipitation in the wine, to achieve stability, reduce sour taste, and round the purpose of cider mouthfeel.
实验7,本实验是实验1的区别仅在于该实验采用单一品种苹果汁发酵苹果酒的方法:Experiment 7, this experiment is the difference from Experiment 1 only in that this experiment uses the method of fermenting cider with a single variety of apple juice:
本实验的步骤(1)中采用常用于苹果酒酿造的苹果(例如:红富士)榨汁;同时破碎过程中加入偏重亚硫酸钾和VC后不采用二氧化碳保护。步骤(5)中未根据发酵液的比重选择循环的方式;步骤(6)中采用的酒渣分离方式是膜过滤,步骤(7)下胶处理操作中未加入蛋清粉。In step (1) of this experiment, apples commonly used in cider brewing (for example: Red Fuji) were used to extract juice; at the same time, potassium metabisulfite and VC were added during the crushing process without carbon dioxide protection. In the step (5), the mode of circulation is not selected according to the specific gravity of the fermented liquid; in the step (6), the distiller's residue separation mode adopted is membrane filtration, and egg white powder is not added in the step (7) gumming treatment operation.
实验8,本实验是实验2的区别仅在于该实验采用单一品种苹果汁与果渣浸渍发酵苹果酒(比例不是1:1)的方法:Experiment 8, this experiment is the difference from Experiment 2 only in that this experiment uses the method of macerating and fermenting cider with a single variety of apple juice and pomace (ratio is not 1:1):
该实施例过程与上面的实施例2步骤基本相同,但是步骤(1)中采用常用于苹果酒酿造的苹果(例如:红富士)榨汁;并且果汁和果渣的比例为苹果酒酿造常用比例2:1(kg/L),且破碎过程中加入偏重亚硫酸钾和VC后不采用二氧化碳保护;步骤(5)中未根据发酵液的比重选择循环的方式;步骤(6)中采用的酒渣分离方式是膜过滤,步骤(7)下胶处理操作中未加入蛋清粉。The process of this embodiment is basically the same as the steps of the above embodiment 2, but in step (1), apples (for example: Red Fuji) commonly used in cider brewing are used to squeeze the juice; and the ratio of juice and pomace is the usual ratio for cider brewing 2:1 (kg/L), and carbon dioxide protection is not adopted after adding potassium metabisulfite and VC in the crushing process; the mode of circulation is not selected according to the specific gravity of the fermented liquid in the step (5); the wine used in the step (6) The slag separation method is membrane filtration, and no egg white powder is added in the gumming treatment operation in step (7).
实验1-8所得苹果酒的感官评定比较如下:The sensory evaluations of the cider obtained in experiments 1-8 are compared as follows:
香味感官层次和饮食口感的评定方式为5人闻、品尝后,按1-5分评分后的得分的平均值,以5分为满分。The evaluation method of aroma sensory level and food taste is the average value of the scores of 1-5 points scored by 5 people after smelling and tasting, with 5 being the full mark.
上面所述只是为了说明本发明,应该理解为本发明并不局限于以上实施例,符合本发明思想的各种变通形式均在本发明的保护范围之内。The above is just to illustrate the present invention, and it should be understood that the present invention is not limited to the above embodiments, and various modifications conforming to the idea of the present invention are within the protection scope of the present invention.
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