CN107354175A - A kind of silver nano material and its biological preparation method and application - Google Patents
A kind of silver nano material and its biological preparation method and application Download PDFInfo
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- CN107354175A CN107354175A CN201710512718.2A CN201710512718A CN107354175A CN 107354175 A CN107354175 A CN 107354175A CN 201710512718 A CN201710512718 A CN 201710512718A CN 107354175 A CN107354175 A CN 107354175A
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Abstract
The invention discloses a kind of silver nano material and its biological preparation method and application.This method is using the endogenetic fungus for being isolated from dendrobium candidum rootFusarium solaniThe extracellular synthesis silver nano material of D07 (preserving number CCTCC NO.M2017145) biologies, reaction condition is gentle, easily controllable, avoids the poisonous or radioreagent in chemistry, physical method, has the advantages of reliable, green.The silver nano material of the present invention is circular or ellipse nano-particle material, and good dispersion, narrow particle size distribution, grain diameter is 16 ~ 46nm, and has good fungistatic effect.The good antibacterial activity having using the silver nano material, silver nano material is applied in the preparation of antibacterial material, had a good application prospect.
Description
Technical field
The present invention relates to the preparation and application field of silver nano material, and in particular to the biological preparation method of silver nano material
With application.
Background technology
Metal nano material is potentially applied in fields such as photoelectron, catalysis, sensor and biologies due to it, it has also become near
One of important directions of more than ten years people research.Wherein, Nano Silver is as a kind of emerging functional material, because with unique thing
Physicochemical property, it is standby in the research field of nano photoelectric device, biomarker, surface-enhanced Raman and biological medicine composite
It is concerned.
Antibacterial material can be divided into natural, organic and inorganic antibacterial material three major types type.Natural biological antibacterial material derives from
Natural extract, it is nontoxic or less toxic, but persistence is poor, and the limitation of condition is subject to processing, the first choice of bacteriostatic agent can't be turned into.
Organic antibacterial material is broadly divided into organic acid, phenols, quaternary ammonium salt etc., by act on bacterium cell membrane and cell membrane,
Biochemical reaction enzyme, inhereditary material etc., suppress the growth and breeding of harmful bacteria, mould.The shortcomings that organic antibacterial material is stable
Poor, the facile hydrolysis of property, can produce secondary pollution, toxic, it is difficult to control slow release.The shortcomings that in order to avoid organic antibacterial material,
Researcher has gradually turned one's attention to inorganic antibacterial material.
Inorganic antibacterial material is mainly the bacteriostasis property possessed in itself by metal, chooses the porous materials such as zeolite, silica gel
The methods of material is used as matrix, above-mentioned metal is passed through into physical absorption or ion exchange is carried in the surface or duct of matrix, then
It is introduced into antibacterial product.Inorganic antibacterial material advantages, most notably toxicity is low, heat-resist, the duration
It is long, has a broad antifungal spectrum, it is the good antiseptic kind of commercial promise.Inorganic bacteriostatic agent can be divided into catalysis material, metal ion
Material, rare-earth-type material, natural crystal, shell.Currently widely used Ag+、Cu2+And Zn2+As the inorganic antibacterial material of metal system
The metallic component of material.Micro Ag+、Cu2+And Zn2+It is beneficial to human body, but there is destruction, silver ion to microorganism
Bacteriostasis property it is most strong, zinc ion is most weak, so silver-based inorganic bacteriostatic agent is most widely used.Silver is a kind of time-honored
Bacteriostatic agent, people begin to preserve food using silverware tool in ancient times, and to early 20th century, silver nitrate solution is just by surgery founder
One of Halstead be used for handle wound and scald.
The preparation of inorganic antibacterial material has physics, chemistry, biological method at present, because chemically and physically method preparation is related to
To poisonous or radioreagent, and biological method turns into silver nano technology development in recent years because it has reliable, Green Features
Trend.
The content of the invention
It is an object of the invention to provide a kind of silver nano material, the silver nano material is circular or ellipse nanometer
Grain material, good dispersion, narrow particle size distribution, grain diameter is 16~46nm, and has good fungistatic effect.
The present invention also aims to provide a kind of biological preparation method of described silver nano material, this method, which uses, divides
From endogenetic fungus Fusarium solani D07 (preserving number CCTCC NO.M2017145) biological born of the same parents from dendrobium candidum root
The outer synthesis silver nano material, reaction condition are gentle, easily controllable.
The present invention also aims to provide a kind of application of the described silver nano material in antibacterial material is prepared, due to
The silver nano material has good antibacterial activity, therefore the silver nano material is applied in the preparation of antibacterial material, tool
There is good application prospect.
The purpose of the present invention is achieved through the following technical solutions.
A kind of biological preparation method of silver nano material, including following flow：
Fusarium solani D07 bacterial strains activation → seed culture fluid → fermented and cultured → suction filtration obtains mycelia → mycelia
Culture → suction filtration obtains filtrate → addition AgNO in aseptic deionized water3Reaction → material obtains.
A kind of biological preparation method of silver nano material, specifically comprises the following steps：
(1) after dendrobium candidum endogenetic fungal bacterial strain is activated, seed liquor culture is carried out, obtained seed liquor is sent out again
Ferment culture；
(2) nutrient solution that step (1) finally gives is filtered, aseptic deionized water rinses, and the mycelia of acquisition is in nothing
After being cultivated in bacterium deionized water, filter again, AgNO is added into obtained filtrate3Reacted；After reaction terminates, centrifugation,
Precipitation is collected, obtains the silver nano material.
Further, in step (1), the dendrobium candidum endogenetic fungal bacterial strain is isolated from dendrobium candidum root, latin name
For Fusarium solani D07, China typical culture collection center (Wuhan City, Hubei Province is preserved on March 27th, 2017
City Wuchang road Luo Jia Shan Wuhan University, postcode 430072), preserving number is CCTCC NO.M2017145.
Further, in step (1), the activation is aseptically, using test tube slant cultural method in 28 ± 1
DEG C activation culture 48~72 hours, culture medium is potato dextrose agar (PDA).
Further, in step (1), the seed liquor culture is aseptically, using potato glucose water planting
Base (PDB) is supported, in 28 ± 1 DEG C, rotating speed 120rpm shaking table cultures 48~72 hours.
Further, in step (1), the fermented and cultured is aseptically, by obtained seed liquor according to 10v%
Inoculum concentration access fluid nutrient medium in, cultivated 3~4 days under the conditions of 28 ± 1 DEG C of temperature, rotating speed 120rpm.
Further, in step (2), the aseptic deionized water uses Milli-Q deionized waters.
Further, in step (2), the culture in aseptic deionized water is aseptically temperature 28 ± 1
DEG C, 24~48h is cultivated under the conditions of rotating speed 120rpm.
Further, in step (2), the addition AgNO3Carry out in course of reaction, keep the AgNO added3Concentration exist
1mmol/L。
Further, in step (2), the reaction is 24~48h of reaction under conditions of room temperature, rotating speed 120rpm.
Further, in step (2), the centrifugation is in 4 DEG C of temperature, 12000g centrifugations 20min.
The process cultivated in the activation, seed liquor culture, fermented and cultured, suction filtration and aseptic deionized water, be
Carried out under aseptic condition.
The preparation principle of silver nano material of the present invention：During the grown cultures of Fusarium solani D07 bacterial strains,
Fusarium solani D07 bacterial strains can produce reductase, and the reductase promotes Ag+Ion is reduced to Ag, generates described
Silver nano material.
A kind of silver nano material made from the preparation method as described in any of the above-described, for circular or oval nano particle material
Material, the particle diameter of particle is 16~46nm.
A kind of described silver nano material has good antibacterial activity, for including candida tropicalis, waxy brood cell
Test bacterium including bacillus, bacillus subtilis, staphylococcus aureus, Escherichia coli, Bacillus alcaligenes and salmonella is equal
There is good inhibiting effect；The silver nano material is applied to the preparation of antibacterial material, includes the exploitation system of antibacterial coating
It is standby.
Compared with prior art, the invention has the advantages that and beneficial effect：
(1) the invention provides the biological preparation method of silver nano material, technical process is simple and easy, reaction condition temperature
With, it is easily controllable, and silver nano material is prepared using biological method, avoids the poisonous or radioactivity in chemistry, physical method
Reagent, there is the advantages of reliable, green；
(2) silver nano material of the invention is circular or ellipse nano-particle material, good dispersion, size distribution
It is narrow, and there is good fungistatic effect, silver nano material is applied in the preparation of antibacterial material, before there is good application
Scape.
Brief description of the drawings
Fig. 1 is to add AgNO in embodiment 13440nm feature of the cell filtrate under ultraviolet-visible spectrophotometer inhale
Receive curve map；
Fig. 2 is the transmission electron microscope figure of the silver nano material prepared in embodiment 1.
Embodiment
Technical solution of the present invention is further elaborated below in conjunction with specific embodiments and the drawings, but the invention is not restricted to
This.
The experimental method of unreceipted actual conditions in the following example, generally according to institute in normal condition, laboratory manual
The condition stated or according to the condition proposed by manufacturer.
The dendrobium candidum endogenetic fungal bacterial strain Fusarium solani D07 used in the specific embodiment of the invention are in 2017
On March 27, in is preserved in China typical culture collection center (Wuhan City, Hubei Province Wuchang road Luo Jia Shan Wuhan University, postcode
430072), preserving number is CCTCC NO.M2017145；The ribosomes the Internal Transcribed Spacer (ITS) of the bacterial strain and 5.8S ribosomes
RNA encoding genes (5.8SrDNA) are as shown in SEQ ID No.1.
The bacteriostatic activity of silver nano material in the specific embodiment of the invention carries out test observation with the following method, specifically
Flow is as follows：
Cause of disease bacteria strain activation → seed liquor culture → flat board coating inoculation → filter paper containing silver nano material inoculation → training
Support → Bacteriostatic Effect.
Embodiment 1
The biology preparation of silver nano material, preparation flow are as follows：
Fusarium solani D07 bacterial strains activation → seed culture fluid → fermented and cultured → suction filtration obtains mycelia → mycelia
Culture → suction filtration obtains filtrate → addition AgNO in aseptic deionized water3Reaction → material obtains.
Specific preparation process comprises the following steps：
(1) dendrobium candidum endogenetic fungal bacterial strain Fusarium solani D07 strains are taken, aseptically, with inoculation
The a small amount of mycelia of pin picking, sterilized solid PDA medium test tube is accessed, in 28 ± 1 DEG C of activation cultures 72 hours；
(2) strain after activation culture is taken, aseptically, in sterilized liquid PDB seed culture mediums of transferring,
In 28 ± 1 DEG C, 120rpm shaking table cultures 72 hours, seed liquor is obtained；
(3) aseptically, accessed by 10v% (percent by volume) inoculum concentration in 500ml fluid nutrient mediums, in 28 ±
1 DEG C, 120rpm shaker fermentations culture 3 days；
(4) by zymotic fluid in vacuum under sterile conditions filter, mycelium aseptic water washing, remove remaining medium into
Point；
(5) mycelium (weight in wet base 20g) of harvest is re-seeded into the 500ml containing 200ml Milli-Q deionized waters
In conical flask, 28 ± 1 DEG C, 120rpm cultivate 24 hours；
(6) mycelium is filtered to remove, is collecting the addition AgNO of nutrient solution3, and ensure AgNO3Ultimate density is 1mmol/
L, reaction solution is in 25 DEG C, 120rpm reactions 24h；Reaction solution in ultraviolet-uisible spectrophotometer 200-800nm length scannings, it is determined that
There is silver nano material generation；
Add AgNO3440nm characteristic absorptions curve map such as Fig. 1 under ultraviolet-visible spectrophotometer of cell filtrate
It is shown, as shown in Figure 1, AgNO is added in cell filtrate3With adding AgNO in cell thalline3Sample all has at 440nm afterwards
One absworption peak, this is just the same with the characteristic resonances absworption peak of Nano Silver, it was demonstrated that has the two samples to have silver nano material shape
Into；And control is used as, single cell filtrate and AgNO3Do not absorbed at 440nm, show the generation of no Nano Silver.
(7) after reaction terminates, reaction solution collects precipitation in 20 minutes in 12000g, 4 DEG C of centrifugations, transmission electron microscopy after drying
Mirror characterizes structure.
The transmission electron microscope figure of the silver nano material of preparation is as shown in Fig. 2 as shown in Figure 2, the silver nanoparticle material of preparation
Expect that for circular or oval granular materials, grain diameter is in 16~46nm.
Comparative example 1
The step of comparative example blank control group preparation nano material, is same as Example 1, and difference is, blank control group is not
Add AgNO3Reacted.
Embodiment 2
The test that the nano material that embodiment 1 and comparative example 1 are obtained carries out bacteriostatic activity is observed, and bacteriostatic activity evaluation is adopted
It is as follows with filter paper enzyme, flow：
Cause of disease bacteria strain activation → seed liquor culture → flat board coating inoculation → filter paper containing silver nanoparticle inoculation → culture →
Bacteriostatic Effect.
Bacteriostatic activity is observed, and is specifically comprised the following steps：
(1) 7 kinds of common pathogens of selection are taken：Escherichia coli, staphylococcus aureus, bacillus subtilis, Salmonella
Bacterium, Bacillus cereus, candida tropicalis and Bacillus alcaligenes strain, aseptically, with a small amount of bacterium colony of transfer needle picking,
Sterilized beef-protein medium (solid-state) test tube is accessed, is activated 24 hours in 37 ± 1 DEG C；
(2) strain after activation culture is taken, aseptically, is transferred into sterilized liquid beef extract-peptone liquid
In culture medium (liquid), in 37 ± 1 DEG C, 160rpm shaking table cultures 24 hours, seed liquor is obtained；
(3) aseptically, access in 10ml liquid LB plating mediums, shake by 2v% (percent by volume) inoculum concentration
Dynamic flat board, is well mixed seed liquor and LB fluid nutrient mediums, its solidification is treated in superclean bench；
(4) by by the silver nano material colloidal solution of the extracellular synthesis of dendrobium candidum endogenetic fungus D07 in embodiment 1
The filter paper (Φ=0.6cm, having sterilized) of immersion, filter paper (Φ=0.6cm, having sterilized) is soaked in pair using same method
In the colloidal solution of ratio 1；It is sterile air-dry after, be seeded in coating pathogenic bacteria beef extract-peptone plating medium, 37 DEG C fall
Put culture 48 hours；
(5) the test bacterium culture dish after making is placed in 37 DEG C of incubator to be observed after incubated 24h, and it is antibacterial to survey its
The size of circle.
The observed result of inhibition zone is as shown in table 1.
The nano material antibacterial activity result of the embodiment 1 of table 1 and comparative example 1
As shown in Table 1, the silver nano material that embodiment 1 synthesizes is long-range to the inhibition zone of 7 kinds of selected common pathogens
In the inhibition zone of the nano material of comparative example 1, it is bright to illustrate that silver nano material that embodiment 1 synthesizes has to common pathogen in selected 7
Aobvious resistance, have the function that significantly to suppress common pathogen.
It should be appreciated that embodiments above is only that the elaboration further understood to technical solution of the present invention, and do not have to
In limitation the scope of the present invention.In addition, after the content that the present invention is told about has been read, those skilled in the art implement to the present invention
Various changes that example is made, the equivalence of modifications or substitutions, the model that will equally fall within the application appended claims and limited
Enclose.
SEQUENCE LISTING
<110>South China Science ＆ Engineering University
<120>A kind of silver nano material and its biological preparation method and application
<130>
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 572
<212> DNA
<213> Fusarium solani D07
<400> 1
tttcctccgg cctttgatat gcttaagttc agcgggtatt cctacctgat ccgaggtcaa 60
cattcagaag ttggggttta acggcgtggc cgcgacgatt accagtaacg agggttttac 120
tactacgcta tggaagctcg acgtgaccgc caatcaattt ggggaacgcg aattaacgcg 180
agtcccaaca ccaagctgtg cttgagggtt gaaatgacgc tcgaacaggc atgcccgcca 240
gaatactggc gggcgcaatg tgcgttcaaa gattcgatga ttcactgaat tctgcaattc 300
acattactta tcgcattttg ctgcgttctt catcgatgcc agaaccaaga gatccgttgt 360
tgaaagtttt gatttattta tggttttact cagaagttac atatagaaac agagtttagg 420
ggtcctctgg cgggccgtcc cgttttaccg ggagcgggct gatccgccga ggcaacaagt 480
ggtatgttca caggggtttg ggagttgtaa actcggtaat gatcctccgc tggttcacca 540
acggagacct tgttacgatt tttttacttc ca 572
Claims (10)
1. a kind of biological preparation method of silver nano material, it is characterised in that comprise the following steps：
（1）After dendrobium candidum endogenetic fungal bacterial strain is activated, seed liquor culture is carried out, obtained seed liquor is subjected to fermentation training again
Support；
（2）By step（1）The nutrient solution finally given is filtered, and aseptic deionized water rinses, and the mycelia of acquisition goes in sterile
After being cultivated in ionized water, filter again, AgNO is added into obtained filtrate3Reacted；After reaction terminates, centrifugation, collect
Precipitation, obtains the silver nano material.
A kind of 2. biological preparation method of silver nano material according to claim 1, it is characterised in that step（1）In, institute
State dendrobium candidum endogenetic fungal bacterial strain and be isolated from dendrobium candidum root, Latin is entitledFusarium solaniD07, preserving number are
CCTCC NO.M2017145。
A kind of 3. biological preparation method of silver nano material according to claim 1, it is characterised in that step（1）In, institute
It is aseptically, to be in 28 ± 1 DEG C of activation cultures 48 ~ 72 hours, culture medium using test tube slant cultural method to state activation
Potato dextrose agar.
A kind of 4. biological preparation method of silver nano material according to claim 1, it is characterised in that step（1）In, institute
It is aseptically, using potato glucose water culture medium, in 28 ± 1 DEG C, rotating speed 120rpm shaking tables to state seed liquor culture
Culture 48 ~ 72 hours.
A kind of 5. biological preparation method of silver nano material according to claim 1, it is characterised in that step（1）In, institute
It is aseptically, obtained seed liquor to be accessed in fluid nutrient medium according to 10v% inoculum concentration, Yu Wen to state fermented and cultured
Cultivated 3-4 days under the conditions of 28 ± 1 DEG C of degree, rotating speed 120rpm.
A kind of 6. biological preparation method of silver nano material according to claim 1, it is characterised in that step（2）In, institute
State aseptic deionized water and use Milli-Q deionized waters；The culture in aseptic deionized water is aseptically temperature
28 ± 1 DEG C, 24 ~ 48h is cultivated under the conditions of rotating speed 120rpm.
A kind of 7. biological preparation method of silver nano material according to claim 1, it is characterised in that step（2）In, institute
State and add AgNO3Carry out in course of reaction, keep the AgNO added3Concentration in 1mmol/L；It is described reaction be temperature room temperature,
24 ~ 48h is reacted under conditions of rotating speed 120rpm.
A kind of 8. biological preparation method of silver nano material according to claim 1, it is characterised in that step（2）In, institute
It is in 4 DEG C of temperature, 12000g centrifugations 20min to state centrifugation.
9. a kind of silver nano material as made from the preparation method described in any one of claim 1 ~ 8, it is characterised in that for circle
Or oval nano-particle material, the particle diameter of particle is 16 ~ 46nm.
10. a kind of silver nano material described in claim 9 is applied to the preparation of antibacterial material.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112159765A (en) * | 2020-10-22 | 2021-01-01 | 华侨大学 | Ageratum endophytic fungus Letenadraea sp.WZ07 and application thereof in nano silver synthesis |
| CN114806887A (en) * | 2022-03-28 | 2022-07-29 | 云南大学 | Fungus strain for promoting germination of dendrobium chrysosma and application |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888428A (en) * | 2011-07-21 | 2013-01-23 | 中国科学院过程工程研究所 | Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62 |
| CN104762329A (en) * | 2015-02-06 | 2015-07-08 | 东南大学 | Method using fusarium venenatum 1281-2 to synthesize nano-silver |
| CN105935781A (en) * | 2016-06-06 | 2016-09-14 | 江苏省农业科学院 | Biological method for preparing nano-silver |
-
2017
- 2017-06-29 CN CN201710512718.2A patent/CN107354175B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102888428A (en) * | 2011-07-21 | 2013-01-23 | 中国科学院过程工程研究所 | Method for synthesizing nano silver by utilizing Bacillus amyloliquefaciensBacillus amyloliquefaciens LSSE-62 |
| CN104762329A (en) * | 2015-02-06 | 2015-07-08 | 东南大学 | Method using fusarium venenatum 1281-2 to synthesize nano-silver |
| CN105935781A (en) * | 2016-06-06 | 2016-09-14 | 江苏省农业科学院 | Biological method for preparing nano-silver |
Non-Patent Citations (1)
| Title |
|---|
| SWAPNIL C. GAIKWAD等: "Screening of Different Fusarium Species to Select Potential Species for the Synthesis of Silver Nanoparticles", 《J. BRAZ. CHEM. SOC.》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112159765A (en) * | 2020-10-22 | 2021-01-01 | 华侨大学 | Ageratum endophytic fungus Letenadraea sp.WZ07 and application thereof in nano silver synthesis |
| CN112159765B (en) * | 2020-10-22 | 2022-06-07 | 华侨大学 | Ageratum endophytic fungus Letenadraea sp.WZ07 and application thereof in nano silver synthesis |
| CN114806887A (en) * | 2022-03-28 | 2022-07-29 | 云南大学 | Fungus strain for promoting germination of dendrobium chrysosma and application |
| CN114806887B (en) * | 2022-03-28 | 2023-09-05 | 云南大学 | Fungus strain for promoting germination of Jinsha Jiang Danhu and application |
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| CN107354175B (en) | 2021-05-18 |
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