CN107349251A - A kind of method that contraceptive is made using potyvirus stalk - Google Patents
A kind of method that contraceptive is made using potyvirus stalk Download PDFInfo
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- CN107349251A CN107349251A CN201710683614.8A CN201710683614A CN107349251A CN 107349251 A CN107349251 A CN 107349251A CN 201710683614 A CN201710683614 A CN 201710683614A CN 107349251 A CN107349251 A CN 107349251A
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- Prior art keywords
- potyvirus
- stalk
- contraceptive
- ethanol
- pyrolyzed
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/42—Cucurbitaceae (Cucumber family)
- A61K36/428—Trichosanthes
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
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- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to pharmaceutical technology field, and in particular to a kind of method that contraceptive is made using potyvirus stalk.Comprise the following steps：By the cleaning of potyvirus stalk, sterilizing, drying, lactobacillus acidophilus bacterium solution is atomized, uniformly it is sprayed on potyvirus stalk, potyvirus stalk that fermentation process obtains is pyrolyzed being passed through in retort, backflow extraction is mixed with ethanol, extract solution is concentrated, the present invention can all be killed sperm in 5 seconds using the obtained contraception medicament of potyvirus stalk, sperm is lost mobility, and nontoxic, harmless, non-stimulated to human vagina's mucous membrane, long-term use does not produce any negative effect to human body.
Description
Technical field
The present invention relates to pharmaceutical technology field, and in particular to a kind of method that contraceptive is made using potyvirus stalk.
Background technology
Potyvirus, also known as snakegourd, because be after its maturation inside yellow be thin pulp thus alias dung wax gourd, be Curcurbitaceae perennial grass
Matter liana, it is grown in height above sea level 100-1800 rice weathers gentle mountain valley thick forest and hillside fields shrubbery, widely collecting scenery with hills and waters, it is " clever
Gas ".It is a kind of rare Chinese medicine, according to《Compendium of Materia Medica》、《Dictionary of medicinal plant》、《Chinese medicine is complete works of》The traditional Chinese medicines such as Chinese medicinal herbal
Document is recorded:Skin, seed, root can be used as medicine, have moistening lung for removing phlegm, decreasing internal heat and relieving cough, relieving stuffiness of the chest by dispersing aggregation of pathogens, detumescence toxic removing, relax bowel
Effect.Potyvirus is various in style, now can be divided into two major classes, i.e. edible melon and medicinal potyvirus with purposes value.Edible melon, mainly
Its ripe seed is taken, its full seed, meat fertilizer, is in edible melon seeds containing abundant fat and protein based on frying food
Good merchantable brand, and with health role, medicinal potyvirus, take its ripe seed, can equally make frying food, but quality compares and wants poor one
A bit, its fresh fruit after growing up mainly is taken, Chinese medicine material is made after drying processing.It is usually used in treating the hot cough of phlegm, heart pain uncomfortable in chest, the heart twists
The diseases such as pain, take its root that Chinese medicine i.e. " root of Chinese trichosanthes " is made, are TCM Gynecology Labor-inducing medicines, mainly with smallpox powder-injection intramuscular injection, can make
Chorionic villi of placenta trophocyte degeneration necrosis and cause miscarriage, yet there are no the report in terms of useful potyvirus stalk makes contraceptive
Road.
The content of the invention
It is an object of the invention to provide a kind of method that contraceptive is made using potyvirus stalk.
The technical solution adopted by the present invention is a kind of method that contraceptive is made using potyvirus stalk, including following step
Suddenly：
（1）Potyvirus stalk is cleaned up, and thoroughly sterilizing at high temperature, dry to water content and exist under 35-38 DEG C of hot blast
8-10%；
（2）Lactobacillus acidophilus bacterium solution is atomized, is uniformly sprayed on potyvirus stalk, by potyvirus stalk water Weight control in 5.5-
5.8%, fermentation process 26-30 hours at being then 28-30 DEG C in temperature；
（3）The potyvirus stalk that fermentation process obtains is being passed through in retort, under anaerobic, is being pyrolyzed at 150-180 DEG C
Handle 2-2.5 hours；
（4）Obtained potyvirus stalk will be pyrolyzed with 50-60% ethanol by 1:8 volume ratio is well mixed, and is placed in microwave reflux
In, 30-35 minutes are extracted under 120-130W microwave power, take filtrate, ethanol is reclaimed, extract solution is concentrated into solid content and contained
Measure in 25-28%, obtain concentrate and suppository is made for practising contraception.
Viable count is in 3-5 × 10 in the lactobacillus acidophilus bacterium solution9CFU/mL。
Beneficial aspects of the present invention are that the present invention can be complete by sperm in 5 seconds using the obtained contraception medicament of potyvirus stalk
Portion kills, and sperm is lost mobility, and nontoxic, harmless, non-stimulated to human vagina's mucous membrane, long-term use to human body not
Produce any negative effect.
Embodiment
Embodiment 1：
A kind of method that contraceptive is made using potyvirus stalk, is comprised the following steps：
（1）Potyvirus stalk is cleaned up, and thoroughly sterilizing at high temperature, dried under 35 DEG C of hot blast to water content 8%；
（2）Lactobacillus acidophilus bacterium solution is atomized, is uniformly sprayed on potyvirus stalk, by potyvirus stalk water Weight control 5.5%,
Then fermentation process 26 hours at being 28 DEG C in temperature；
（3）The potyvirus stalk that fermentation process obtains is being passed through in retort, under anaerobic, the pyrolysis processing 2 at 150 DEG C
Hour；
（4）Obtained potyvirus stalk and 50% ethanol will be pyrolyzed by 1:8 volume ratio is well mixed, and is placed in microwave reflux,
Extracted 30 minutes under 120W microwave power, take filtrate, reclaim ethanol, extract solution is concentrated into solid content 25%, obtained
Suppository is made to concentrate to be used to practise contraception.
Viable count is 3 × 10 in the lactobacillus acidophilus bacterium solution9CFU/mL。
Embodiment 2：
A kind of method that contraceptive is made using potyvirus stalk, is comprised the following steps：
（1）Potyvirus stalk is cleaned up, and thoroughly sterilizing at high temperature, dried under 38 DEG C of hot blast to water content 10%；
（2）Lactobacillus acidophilus bacterium solution is atomized, is uniformly sprayed on potyvirus stalk, by potyvirus stalk water Weight control 5.8%,
Then fermentation process 30 hours at being 30 DEG C in temperature；
（3）The potyvirus stalk that fermentation process obtains is being passed through in retort, under anaerobic, the pyrolysis processing at 180 DEG C
2.5 hour；
（4）Obtained potyvirus stalk and 60% ethanol will be pyrolyzed by 1:8 volume ratio is well mixed, and is placed in microwave reflux,
Extracted 35 minutes under 130W microwave power, take filtrate, reclaim ethanol, extract solution is concentrated into solid content 28%, obtained
Suppository is made to concentrate to be used to practise contraception.
Viable count is 5 × 10 in the lactobacillus acidophilus bacterium solution9CFU/mL。
Innovative point of the present invention is further illustrated below in conjunction with specific contrast test：
Experiment is divided into experimental group, control group 1, control group 2,3 four groups of control group, takes the same place of production, same plot to produce potyvirus straw
Stalk amounts to 5kg, is randomly divided into 5 groups, i.e. experimental group, control group 1, control group 2, control group 3, control group 4, every group of 1kg；
After each group potyvirus stalk is fully sterilized, dry to water content in 8-10%；
Experimental group potyvirus stalk is handled according to the mode of embodiment 1；
The potyvirus stalk of control group 1 cancellation step on the basis of embodiment 1（2）Processing；
The potyvirus stalk of control group 2 cancellation step on the basis of embodiment 1（3）Processing；
The potyvirus stalk of control group 3 and 50% ethanol press 1:8 volume ratio is well mixed, and is placed in microwave reflux, 120W's
Extracted 30 minutes under microwave power, take filtrate, reclaimed ethanol, extract solution is concentrated into solid content 25%；
The potyvirus stalk concentrate that each group obtains is mixed with seminal fluid by 0.2-0.5: 1 volume ratio, count each group eradicative rate and
The time is killed, as a result see the table below 1：
| Group | Eradicative rate（%） | Kill the time（s） |
| Experimental group | 100 | 3 |
| Control group 1 | 32 | 12 |
| Control group 2 | 66 | 15 |
| Control group 3 | 5 | 20 |
As shown in Table 1, the concentrate obtained by the PROCESS FOR TREATMENT potyvirus stalk of embodiment 1 has to sperm preferably kills effect, and does not have
What extremely short time.
Claims (2)
- A kind of 1. method that contraceptive is made using potyvirus stalk, it is characterised in that comprise the following steps：（1）Potyvirus stalk is cleaned up, and thoroughly sterilizing at high temperature, dry to water content and exist under 35-38 DEG C of hot blast 8-10%；（2）Lactobacillus acidophilus bacterium solution is atomized, is uniformly sprayed on potyvirus stalk, by potyvirus stalk water Weight control in 5.5- 5.8%, fermentation process 26-30 hours at being then 28-30 DEG C in temperature；（3）The potyvirus stalk that fermentation process obtains is being passed through in retort, under anaerobic, is being pyrolyzed at 150-180 DEG C Handle 2-2.5 hours；（4）Obtained potyvirus stalk will be pyrolyzed with 50-60% ethanol by 1:8 volume ratio is well mixed, and is placed in microwave reflux In, 30-35 minutes are extracted under 120-130W microwave power, take filtrate, ethanol is reclaimed, extract solution is concentrated into solid content and contained Measure in 25-28%, obtain concentrate and suppository is made for practising contraception.
- A kind of 2. method that contraceptive is made using potyvirus stalk according to claim 1, it is characterised in thatViable count is in 3-5 × 10 in the lactobacillus acidophilus bacterium solution9CFU/mL。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710683614.8A CN107349251A (en) | 2017-08-11 | 2017-08-11 | A kind of method that contraceptive is made using potyvirus stalk |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710683614.8A CN107349251A (en) | 2017-08-11 | 2017-08-11 | A kind of method that contraceptive is made using potyvirus stalk |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107349251A true CN107349251A (en) | 2017-11-17 |
Family
ID=60287139
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710683614.8A Pending CN107349251A (en) | 2017-08-11 | 2017-08-11 | A kind of method that contraceptive is made using potyvirus stalk |
Country Status (1)
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6066338A (en) * | 1993-10-01 | 2000-05-23 | Legere Pharmaceuticals, Ltd. | Method of using lectins for contraception |
-
2017
- 2017-08-11 CN CN201710683614.8A patent/CN107349251A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6066338A (en) * | 1993-10-01 | 2000-05-23 | Legere Pharmaceuticals, Ltd. | Method of using lectins for contraception |
Non-Patent Citations (4)
| Title |
|---|
| 刘金娜: "瓜蒌的化学成分和药理作用研究进展", 《中药材》 * |
| 刘飞: "栝楼雄株茎叶的化学成分研究", 《中国优秀硕士学位论文全文数据库医药卫生科技辑》 * |
| 李卫民: "《中草药抗生育研究》", 30 June 1999, 中国医药科技出版社 * |
| 贾天柱: "《中药炮制化学》", 31 October 2015, 上海科学技术出版社 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20171117 |