CN107334137A - The supervising device of fermented food fermented quality - Google Patents
The supervising device of fermented food fermented quality Download PDFInfo
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- CN107334137A CN107334137A CN201710511376.2A CN201710511376A CN107334137A CN 107334137 A CN107334137 A CN 107334137A CN 201710511376 A CN201710511376 A CN 201710511376A CN 107334137 A CN107334137 A CN 107334137A
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- illustrative plates
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
- A23L27/50—Soya sauce
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention provides a kind of supervising device of fermented food fermented quality.The supervising device includes strain DNA collection of illustrative plates module, culture propagation collection of illustrative plates module, the first comparing module and the second comparing module, and strain DNA collection of illustrative plates module is used for the DNA collection of illustrative plates for providing dominant bacteria used in fermentation；Culture propagation collection of illustrative plates module is used for the growth and breeding dynamic collection of illustrative plates for providing dominant bacteria；First comparing module is used to the DNA collection of illustrative plates of bacteria to be tested being compared, so as to judge whether bacteria to be tested is dominant bacteria；Second comparing module is used for when bacteria to be tested is dominant bacteria, the growth and breeding dynamic collection of illustrative plates of bacteria to be tested during the fermentation is compared with the growth and breeding dynamic collection of illustrative plates of the dominant bacteria in culture propagation collection of illustrative plates module, so as to judge whether the growth and breeding of bacteria to be tested is abnormal.The supervising device is the supervising device of automation, promptness and real-time that fermented food monitors process is improved, to realize that scale fermenting and producing is laid a good foundation.
Description
Technical field
The present invention relates to quality monitoring field, in particular to a kind of supervising device of fermented food fermented quality.
Background technology
In life, having numerous food product is prepared by fermentation, if soy sauce is using pure grain as raw material, is sent out through microorganism
Ferment forms.Fermentation technique forges after more than one thousand years, very ripe, there is independent significance on Chinese food culture.Can be certainly
Dynamicization large-scale development in the market, because being limited in terms of science and technology, it is impossible to play real industry glamour in the world.In actual production
In, also Chang Yin empirically gropes Instructing manufacture, governs the accurate, special of fermentation technique and horizontal, quality management and control and safety guarantee
The fast development of industry.Most microorganism monitoring, nutrition, flavor etc. are assessed and detection, still excessively depends on traditional analysis means,
Govern the high-end development of product quality, also as in production process automation, large-scale development restraining factors.
The content of the invention
It is a primary object of the present invention to provide a kind of supervising device of fermented food fermented quality, to solve prior art
In monitoring means fall behind, restrict fermented food automation, large-scale development the problem of.
To achieve these goals, according to an aspect of the invention, there is provided a kind of prison of fermented food fermented quality
Device is controlled, the supervising device includes：Strain DNA collection of illustrative plates module, culture propagation collection of illustrative plates module, the first comparing module and the second ratio
To module, strain DNA collection of illustrative plates module is used for the DNA collection of illustrative plates for providing dominant bacteria used in fermented food fermentation；Culture propagation
Collection of illustrative plates module is used to provide the growth and breeding dynamic collection of illustrative plates of dominant bacteria during the fermentation；First comparing module is used for will be to be measured
The DNA collection of illustrative plates of strain is compared with the DNA collection of illustrative plates of the dominant bacteria in culture propagation collection of illustrative plates module, so as to judge bacteria to be tested
Whether it is dominant bacteria；Second comparing module is used for when bacteria to be tested is dominant bacteria, by bacteria to be tested during the fermentation
Growth and breeding dynamic collection of illustrative plates be compared with the growth and breeding dynamic collection of illustrative plates of the dominant bacteria in culture propagation collection of illustrative plates module, from
And judge whether the growth and breeding of bacteria to be tested is abnormal.
Further, supervising device also includes：Nutriment collection of illustrative plates module and the 3rd comparing module, nutriment collection of illustrative plates
Module is used for the species and content for providing the nutriment in fermented food, and the 3rd comparing module is used for the growth when bacteria to be tested
When breeding is not present abnormal, bacteria to be tested is fermented to the species and content and nutriment of the nutriment of obtained product to be checked
The species and content of the nutriment in fermented food in collection of illustrative plates module are compared.
Further, dominant bacteria includes any one or more in aspergillus oryzae, saccharomycete and bacillus.
Further, the DNA collection of illustrative plates of dominant bacteria is the DNA spectrum libraries of the specific molecular marker of dominant bacteria；It is preferred that
Any one or more of specific molecular marker in 16S, 26S and ITS.
Further, the DNA collection of illustrative plates of dominant bacteria is DNA denaturing gradient gel electrophoresis collection of illustrative plates.
Further, culture propagation collection of illustrative plates module is what the inoculum density of dominant bacteria during the fermentation changed over time
Dynamic collection of illustrative plates.
Further, fermented food is soy sauce, and preferred nutrient matter includes：Amino acid, protein, amine, vitamin and
Any one or more in isoflavones.
Further, nutriment collection of illustrative plates module obtains nutriment by GC, GC-MS, HPLC and/or HPLC-MS
Species and content.
Further, supervising device also includes：First acquisition module, the first acquisition module are used to obtain bacteria to be tested
DNA collection of illustrative plates.
Further, supervising device also includes：Second acquisition module, the second acquisition module are being sent out for obtaining bacteria to be tested
Growth and breeding dynamic collection of illustrative plates during ferment.
Apply the technical scheme of the present invention, by setting strain DNA collection of illustrative plates module, culture propagation collection of illustrative plates module, the first ratio
To module and the second comparing module, the dominant bacteria related to fermented food fermentation process provided using strain DNA collection of illustrative plates module
The control of the DNA collection of illustrative plates of kind as a comparison, using the first comparing module to used in the fermentation process in real attenuation production
The DNA collection of illustrative plates of bacteria to be tested is compared, so as to detect whether the strain used is the fermented food normal fermentation process
In normal dominant bacteria, if DNA collection of illustrative plates is consistent with the DNA collection of illustrative plates of conventional dominant bacteria, showing the real attenuation process just
Often.If on the contrary, inconsistent, show there is living contaminants during real attenuation, not only influence fermentation efficiency, and may also shadow
Fermented quality is rung, and then the adjustment or termination of fermentation process can be instructed.And when real attenuation process is normal, it can also pass through
Second comparing module and culture propagation collection of illustrative plates module provide the advantage that growth and breeding dynamic of the strain in normal fermentation process
The growth and breeding process of strain during collection of illustrative plates produces to real attenuation is monitored, so as to judge that the growth and breeding of bacteria to be tested is
No exception, it is monitored for the whether abnormal of fermentation process.The supervising device is the supervising device of automation, improves fermentation food
Product monitor the promptness and real-time of process, to realize that scale fermenting and producing is laid a good foundation.
Brief description of the drawings
The Figure of description for forming the part of the application is used for providing a further understanding of the present invention, and of the invention shows
Meaning property embodiment and its illustrate be used for explain the present invention, do not form inappropriate limitation of the present invention.In the accompanying drawings：
Fig. 1 shows the supervising device of the fermented quality of fermented food in a kind of preferred embodiment according to the present invention
Structural representation；
Fig. 2 shows the structural representation of supervising device in second of preferred embodiment according to the present invention；And
Fig. 3 shows the structural representation of supervising device in the third preferred embodiment according to the present invention.
Embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the application can phase
Mutually combination.The present invention is described in detail below in conjunction with embodiment.
As background technology is previously mentioned, the monitoring means of fermented food fermented quality are fallen behind in the prior art, govern
Fermented food automation, large-scale development.In order to improve this present situation, in a kind of typical embodiment of the application, there is provided
A kind of supervising device of fermented food fermented quality, as shown in figure 1, the supervising device includes：Strain DNA collection of illustrative plates module, bacterium
Kind breeding collection of illustrative plates module, the first comparing module and the second comparing module, wherein, strain DNA collection of illustrative plates module, which is used to provide to ferment, eats
The DNA collection of illustrative plates of dominant bacteria used in product fermentation；Culture propagation collection of illustrative plates module is used to provide dominant bacteria during the fermentation
Growth and breeding dynamic collection of illustrative plates；First comparing module is used in the DNA collection of illustrative plates of bacteria to be tested and culture propagation collection of illustrative plates module
The DNA collection of illustrative plates of dominant bacteria is compared, so as to judge whether bacteria to be tested is dominant bacteria；Second comparing module, which is used to work as, to be treated
When survey strain is dominant bacteria, by the growth and breeding dynamic collection of illustrative plates of bacteria to be tested during the fermentation and culture propagation collection of illustrative plates module
In the growth and breeding dynamic collection of illustrative plates of dominant bacteria be compared, so as to judge whether the growth and breeding of bacteria to be tested abnormal.
The above-mentioned supervising device of the application, by setting strain DNA collection of illustrative plates module, culture propagation collection of illustrative plates module, the first ratio
To module and the second comparing module, the dominant bacteria related to fermented food fermentation process provided using strain DNA collection of illustrative plates module
The control of the DNA collection of illustrative plates of kind as a comparison, using the first comparing module to used in the fermentation process in real attenuation production
The DNA collection of illustrative plates of bacteria to be tested is compared, so as to detect whether the strain used is the fermented food normal fermentation process
In normal dominant bacteria, if DNA collection of illustrative plates is consistent with the DNA collection of illustrative plates of conventional dominant bacteria, showing the real attenuation process just
Often.If on the contrary, inconsistent, show there is living contaminants during real attenuation, not only influence fermentation efficiency, and may also shadow
Fermented quality is rung, and then the adjustment or termination of fermentation process can be instructed.And when real attenuation process is normal, it can also pass through
Second comparing module and culture propagation collection of illustrative plates module provide the advantage that growth and breeding dynamic of the strain in normal fermentation process
The growth and breeding process of strain during collection of illustrative plates produces to real attenuation is monitored, so as to judge that the growth and breeding of bacteria to be tested is
No exception, it is monitored for the whether abnormal of fermentation process.The supervising device is the supervising device of automation, improves fermentation food
Product monitor the promptness and real-time of process, to realize that scale fermenting and producing is laid a good foundation.
In order to further improve supervising device monitoring accuracy, in a kind of preferred embodiment of the application, such as Fig. 2 institutes
Show, above-mentioned supervising device also includes nutriment collection of illustrative plates module and the 3rd comparing module, and nutriment collection of illustrative plates module is used to provide
The species and content of nutriment in fermented food, the 3rd comparing module are used for the growth and breeding when bacteria to be tested in the absence of different
Chang Shi, the species and content of the nutriment for the product to be checked that bacteria to be tested is fermented to obtain with nutriment collection of illustrative plates module
The species and content of nutriment in fermented food are compared.
In above preferred embodiment, the figure of the content by further setting the nutriment that can be provided in fermented product
Compose module, with facilitate real attenuation is produced using the 3rd comparing module in nutriment in obtained product to be checked species
And content is monitored, the quality and performance of final fermented product are monitored so as to improve supervising device, improve hair
The automatically-monitored degree of ferment production.
The dominant bacteria of different fermented food fermentation process is different, such as, the dominant bacteria during wine brewing is
S. cervisiae.In a kind of preferred embodiment of the application, during above-mentioned dominant bacteria is primarily directed to making soy sauce
Any one or more in dominant bacteria, including aspergillus oryzae, saccharomycete and bacillus.
Specifically used DNA fragmentation can be according to the difference of strain in the DNA collection of illustrative plates of above-mentioned dominant bacteria, and it is suitable to select
It is different from the DNA target fragments of other strains.In a kind of preferred embodiment of the application, the DNA collection of illustrative plates of above-mentioned dominant bacteria
For the DNA spectrum libraries of the specific molecular marker of dominant bacteria；It is preferred that specific molecular marker is in 16S, 26S and ITS
Any one or more.There is different variable sequences in 16S, 26S and ITS, thus be commonly used to distinguish bacterium in different strains
The molecular labeling of kind.
The concrete form of the DNA collection of illustrative plates of dominant bacteria can be with sequencing result or electrophoresis pattern.From simply intuitively angle
Consider, preferably DNA denaturing gradient gel electrophoresises collection of illustrative plates.
Above-mentioned culture propagation collection of illustrative plates module is the dynamic that the inoculum density of dominant bacteria during the fermentation changes over time
Collection of illustrative plates.
The fermented food that the supervising device of the application is applicable includes but are not limited to soy sauce, wine and vinegar.It is excellent in the application
Elect soy sauce as.When above-mentioned fermented food is soy sauce, the nutriment provided in above-mentioned nutriment collection of illustrative plates module includes：Amino
Any one or more in acid, protein, amine, vitamin and isoflavones.The presence or absence of these nutriments and contain
The height of amount has a major impact to the quality and taste of soy sauce.Thus, by being monitored to these nutriments, can help
Judge the performance quality of fermenting and producing final products.
Specifically, the species and content of the various nutriments included in above-mentioned nutriment collection of illustrative plates module can use
The acquisition of existing method.In a kind of preferred embodiment of the application, sought by GC, GC-MS, HPLC and/or HPLC-MS
Support the species and content of material.The species and content that these methods obtain accurately and reliably, and can be by by these detecting instruments
It is connected with the supervising device of the application, the automatically-monitored of testing result can be realized.
In order to further improve the automaticity of the detection means, in a kind of preferred embodiment of the application, such as Fig. 3
Shown, above-mentioned supervising device also includes the first acquisition module, and the first acquisition module is used for the DNA collection of illustrative plates for obtaining bacteria to be tested.It is logical
The DNA collection of illustrative plates that the first acquisition module obtains the bacteria to be tested determined in real attenuation production is crossed, in order to the first comparing module
The DNA collection of illustrative plates of obtained bacteria to be tested is utilized to be compared with the DNA collection of illustrative plates of dominant bacteria.
Similarly, in order to further improve the automaticity of the detection means, in a kind of preferable implementation of the application
In example, as shown in figure 3, above-mentioned supervising device also includes the second acquisition module, the second acquisition module exists for obtaining bacteria to be tested
Growth and breeding dynamic collection of illustrative plates in fermentation process.The bacterium to be measured determined in real attenuation production is obtained by the second acquisition module
The growth and breeding dynamic collection of illustrative plates of kind during the fermentation, in order to which the second comparing module utilizes obtained bacteria to be tested fermenting
During growth and breeding dynamic collection of illustrative plates of the growth and breeding dynamic collection of illustrative plates with dominant bacteria during the fermentation be compared, so as to
Judge whether the growth and breeding of bacteria to be tested is abnormal.
As can be seen from the above description, the above embodiments of the present invention realize following technique effect：Pass through setting
Strain DNA collection of illustrative plates module, culture propagation collection of illustrative plates module, the first comparing module and the second comparing module, utilize strain DNA collection of illustrative plates moulds
The control of the DNA collection of illustrative plates for the dominant bacteria related to fermented food fermentation process that block provides as a comparison, is compared using first
The DNA collection of illustrative plates of bacteria to be tested used in fermentation process in real attenuation production is compared module, described so as to detect
Whether the strain used is normal dominant bacteria in the fermented food normal fermentation process, if DNA collection of illustrative plates and conventional dominant bacteria
The DNA collection of illustrative plates of kind is consistent, then shows that the real attenuation process is normal.If on the contrary, inconsistent, show have during real attenuation
Living contaminants, not only influence fermentation efficiency, and fermented quality may also be influenceed, so can instruct fermentation process adjustment or
Terminate.And when real attenuation process is normal, it can also be provided by the second comparing module and culture propagation collection of illustrative plates module
Growth and breeding mistake of growth and breeding dynamic collection of illustrative plates of the dominant bacteria in normal fermentation process to the strain in real attenuation production
Journey is monitored, so as to judge whether the growth and breeding of bacteria to be tested is abnormal, is monitored for the whether abnormal of fermentation process.Should
Supervising device is the supervising device of automation, promptness and real-time that fermented food monitors process is improved, to realize scale
Change fermenting and producing to lay a good foundation.
The supervising device of the fermented quality of the fermented food of the application, break through traditional fermented food and be based on microorganism flat board etc.
The monitoring strategies of conventional method, Modern Molecular Biotechnology is incorporated, break through traditional fermented food and referred to based on basic physics and chemistry in national standard
Target analysis strategy, incorporate metabonomic technology.Modern molecular biology technique is integrated by automation, modularization and is applied to
In the industrialized production of traditional fermented food, and the accurately Fast Monitoring device of three big microorganism fungus kind of soy sauce is established, to carry
The large-scale production of the fermented foods such as high soy sauce, there is provided the quality monitoring of automation ensures.
A kind of preferred embodiment of the application by aspergillus oryzae, saccharomycete, the big fungus strain 26S, ITS of bacillus three,
16S, 16-23S rDNA-PCR are detected, and combine metabolism group, GC/GC-MS, HPLC/HPLC-MS technology to soy sauce hair
Microbial metabolism during ferment, quality carry out integrated Dynamic Monitor Technology system and built, to meet automation, extensive raw
The fermentation technique and quality guarantee of production, so for consumer provide more Ensure Liquid, delicious food, safety food.
The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.Within the spirit and principles of the invention, that is made any repaiies
Change, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (10)
1. a kind of supervising device of fermented food fermented quality, it is characterised in that the supervising device includes：
Strain DNA collection of illustrative plates modules, for providing the DNA collection of illustrative plates of dominant bacteria used in fermented food fermentation；
Culture propagation collection of illustrative plates module, for providing the growth and breeding dynamic collection of illustrative plates of the dominant bacteria during the fermentation；
First comparing module, for by the dominant bacteria in the DNA collection of illustrative plates of bacteria to be tested and the culture propagation collection of illustrative plates module
The DNA collection of illustrative plates of kind is compared, so as to judge whether the bacteria to be tested is the dominant bacteria；And
Second comparing module, for when the bacteria to be tested is the dominant bacteria, by the bacteria to be tested in fermentation process
In growth and breeding dynamic collection of illustrative plates and the culture propagation collection of illustrative plates module in the dominant bacteria growth and breeding dynamic collection of illustrative plates
It is compared, so as to judge whether the growth and breeding of the bacteria to be tested is abnormal.
2. supervising device according to claim 1, it is characterised in that the supervising device also includes：
Nutriment collection of illustrative plates module, for providing the species and content of the nutriment in fermented food；And
3rd comparing module, for when the growth and breeding of the bacteria to be tested is not present abnormal, the bacteria to be tested to be fermented
The species and content of the nutriment of obtained product to be checked and the battalion in the fermented food in the nutriment collection of illustrative plates module
The species and content for supporting material are compared.
3. supervising device according to claim 1, it is characterised in that the dominant bacteria includes aspergillus oryzae, saccharomycete
And any one or more in bacillus.
4. supervising device according to any one of claim 1 to 3, it is characterised in that the DNA collection of illustrative plates of the dominant bacteria
For the DNA spectrum libraries of the specific molecular marker of the dominant bacteria；It is preferred that the specific molecular marker be selected from 16S, 26S and
Any one or more in ITS.
5. supervising device according to any one of claim 1 to 3, it is characterised in that the DNA collection of illustrative plates of the dominant bacteria
For DNA denaturing gradient gel electrophoresis collection of illustrative plates.
6. supervising device according to any one of claim 1 to 3, it is characterised in that the culture propagation collection of illustrative plates module
The dynamic collection of illustrative plates changed over time for the inoculum density of the dominant bacteria during the fermentation.
7. the supervising device according to Claims 2 or 3, it is characterised in that the fermented food is soy sauce, preferably described battalion
Foster material includes：Any one or more in amino acid, protein, amine, vitamin and isoflavones.
8. the supervising device according to Claims 2 or 3, it is characterised in that the nutriment collection of illustrative plates module by GC,
At least one of GC-MS, HPLC, HPLC-MS obtain the species and content of the nutriment.
9. supervising device according to any one of claim 1 to 3, it is characterised in that the supervising device also includes：The
One acquisition module, first acquisition module are used for the DNA collection of illustrative plates for obtaining the bacteria to be tested.
10. supervising device according to any one of claim 1 to 3, it is characterised in that the supervising device also includes：
Second acquisition module, second acquisition module are used to obtain the growth and breeding Dynamic Graph of the bacteria to be tested during the fermentation
Spectrum.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101118425A (en) * | 2006-08-01 | 2008-02-06 | 上海中策工贸有限公司 | Nutrition processing system |
| CN103374603A (en) * | 2012-04-12 | 2013-10-30 | 云南天士力帝泊洱生物茶集团有限公司 | Quality control method for fermentation production of Pu'er tea |
| KR20140084598A (en) * | 2012-12-27 | 2014-07-07 | 동아대학교 산학협력단 | Functional Soybean Paste comprising Grain Syrup with Tomato Puree and Method Thereof |
| CN105349690A (en) * | 2015-12-16 | 2016-02-24 | 江南大学 | Solid-state fermented vinegar fermentation process monitoring method based on microbial community acid production index |
-
2017
- 2017-06-27 CN CN201710511376.2A patent/CN107334137A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101118425A (en) * | 2006-08-01 | 2008-02-06 | 上海中策工贸有限公司 | Nutrition processing system |
| CN103374603A (en) * | 2012-04-12 | 2013-10-30 | 云南天士力帝泊洱生物茶集团有限公司 | Quality control method for fermentation production of Pu'er tea |
| KR20140084598A (en) * | 2012-12-27 | 2014-07-07 | 동아대학교 산학협력단 | Functional Soybean Paste comprising Grain Syrup with Tomato Puree and Method Thereof |
| CN105349690A (en) * | 2015-12-16 | 2016-02-24 | 江南大学 | Solid-state fermented vinegar fermentation process monitoring method based on microbial community acid production index |
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