CN107333894A - Yoghourt containing phosphatidyl choline and preparation method thereof - Google Patents
Yoghourt containing phosphatidyl choline and preparation method thereof Download PDFInfo
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- CN107333894A CN107333894A CN201710436586.XA CN201710436586A CN107333894A CN 107333894 A CN107333894 A CN 107333894A CN 201710436586 A CN201710436586 A CN 201710436586A CN 107333894 A CN107333894 A CN 107333894A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/12—Fermented milk preparations; Treatment using microorganisms or enzymes
- A23C9/13—Fermented milk preparations; Treatment using microorganisms or enzymes using additives
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Abstract
The invention discloses a kind of Yoghourt containing phosphatidyl choline and preparation method thereof, wherein, the preparation method includes：1) extracted after ox pancreas is mixed with ethanol, filter off filter residue, extract M1 is made；2) vibrate after adding acetone in obtained extract M1 into step 1), then stand, remove acetone layer, mixture M 2 is made；3) the head product M3 containing phosphatidylserine is made sequentially through ion exchange resin and silicagel column elution in obtained mixture M 2 in step 2)；4) through fermentation after mixing head product M3 and cow's milk, the Yoghourt containing phosphatidyl choline is made.By above-mentioned design, realize with preferable mouthfeel, be suitable for most people daily consumption, be especially suitable for the effect of child's daily consumption.
Description
Technical field
The present invention relates to the production preparation field of the food containing phosphatidyl choline, in particular it relates to containing phosphatidyl choline
Yoghourt and preparation method thereof.
Background technology
Phosphatidyl choline has unique physicochemical property and physiological property because of it so that it is in food using extremely wide
It is general.But in daily life, it often occurs as health products, so that its audient's scope is narrower.Meanwhile phosphatidyl
Choline has good health-care effect to human brain, especially plays the role of to the brain development of child important.
Therefore it provides a kind of have preferable mouthfeel, it is suitable for most people daily consumption, is especially suitable for often eating child's day
The problem of Yoghourt containing phosphatidyl choline and preparation method thereof is urgent need to resolve of the present invention.
The content of the invention
For above-mentioned prior art, it is an object of the invention to overcome, phosphatidyl choline often protect by conduct in the prior art
Strong product occur, so that its audient's scope is narrower, and cause extracting method because higher to its purity requirement when often it is extracted
The problem of cumbersome, there is preferable mouthfeel so as to provide one kind, be suitable for most people daily consumption, it is daily to be especially suitable for child
Edible Yoghourt containing phosphatidyl choline and preparation method thereof.
To achieve these goals, the invention provides a kind of preparation method of the Yoghourt containing phosphatidyl choline, wherein, institute
Stating preparation method includes：
1) extracted after ox pancreas is mixed with ethanol, filter off filter residue, extract M1 is made；
2) vibrate after adding acetone in obtained extract M1 into step 1), then stand, remove acetone layer, be made mixed
Compound M2；
3) obtained mixture M 2 in step 2) is made sequentially through ion exchange resin and silicagel column elution and contains phosphorus
The head product M3 of acyl serine；
4) through fermentation after mixing head product M3 and cow's milk, the Yoghourt containing phosphatidyl choline is made.
Pass through above-mentioned technical proposal, the present invention extract after ox pancreas is mixed with ethanol, take extract, and backward extract
Middle addition acetone vibrates and stood, and acetone layer is removed, further by ion exchange resin and silicagel column elution, then by it
With cow's milk mixing after fermentation, Yoghourt is made, so that by containing a certain amount of phosphatidyl in Yoghourt made from the above method
Choline, and it is suitable for daily consumption, and Yoghourt is good in taste, is suitable for child and eats, and because need not deliberately pay attention to purity,
The cumbersome degree of production is greatly reduced, improves production efficiency.
Other features and advantages of the present invention will be described in detail in subsequent specific embodiment part.
Embodiment
The embodiment of the present invention is described in detail below.It is it should be appreciated that described herein specific
Embodiment is merely to illustrate and explain the present invention, and is not intended to limit the invention.
The end points of disclosed scope and any value are not limited to the accurate scope or value herein, these scopes or
Value should be understood to comprising the value close to these scopes or value.For number range, between the endpoint value of each scope, respectively
It can be combined with each other between the endpoint value of individual scope and single point value, and individually between point value and obtain one or more
New number range, these number ranges should be considered as specific open herein.
The invention provides a kind of preparation method of the Yoghourt containing phosphatidyl choline, wherein, the preparation method includes：
1) extracted after ox pancreas is mixed with ethanol, filter off filter residue, extract M1 is made；
2) vibrate after adding acetone in obtained extract M1 into step 1), then stand, remove acetone layer, be made mixed
Compound M2；
3) obtained mixture M 2 in step 2) is made sequentially through ion exchange resin and silicagel column elution and contains phosphorus
The head product M3 of acyl serine；
4) through fermentation after mixing head product M3 and cow's milk, the Yoghourt containing phosphatidyl choline is made.
The present invention takes extract by being extracted after ox pancreas mix with ethanol, and addition acetone shakes in extract backward
Swing and stand, remove acetone layer, further by ion exchange resin and silicagel column elution, after then it is mixed with cow's milk
Fermentation, Yoghourt is made, so that by containing a certain amount of phosphatidyl choline in Yoghourt made from the above method, and be suitable for
Daily consumption, and Yoghourt is good in taste, is suitable for child and eats, and because without deliberately paying attention to purity, greatly reducing life
Cumbersome degree is produced, improves production efficiency.
In a kind of preferred embodiment of the present invention, relative to the ox pancreas of 10 parts by weight, the ethanol
Dosage is 100-300 parts by weight, and the dosage of the acetone is 200-400 parts by weight, and the dosage of the cow's milk is 80-150 weight
Part.
In further preferred embodiment, in order to further improve the mouthfeel of obtained Yoghourt, and further raising pair
The utilization rate of raw material, relative to the ox pancreas of 10 parts by weight, the dosage of the ethanol is 150-250 parts by weight, described third
The dosage of ketone is 250-350 parts by weight, and the dosage of the cow's milk is 100-120 parts by weight.
To be operated according to this area usual manner, extracting condition can be not construed as limiting for extraction in step 1), certainly,
In a kind of preferred embodiment of the present invention, in order that extracting effect is more preferable, extraction time is 5-10h in step 1), leaching
Temperature raising degree is 30-40 DEG C.
In further preferred embodiment, vibration is sonic oscillation in step 2), and duration of oscillation is 5-15min, quiet
It is 20-60min to put the time.
In order to further improve the content of phosphatidyl choline in obtained Chinese medicine preparation, through ion exchange resin in step 3)
Elution time be 1-5min, the elution time through silicagel column is 3-10min.
Fermentation can be operated according to this area usual manner in step 4), for example, one kind in the present invention is preferable
In embodiment, fermentation temperature can be selected as 35-40 DEG C in step 4), and fermentation time can be selected as 8-15h.
In order to further improve mouthfeel, in a kind of embodiment being more highly preferred to of the present invention, fermented in step 4)
After can also include add jam be mixed.
Present invention also offers a kind of Yoghourt containing phosphatidyl choline according to made from preparation method described above.
The present invention will be described in detail by way of examples below.In following examples, the ox pancreas, the second
Alcohol, the acetone and the cow's milk are conventional commercial products.
Embodiment 1
1) it is placed under conditions of temperature is 30 DEG C and extracts after the ox pancreas of 10 parts by weight is mixed with the ethanol of 150 parts by weight
5h, filter residue is filtered off, extract M1 is made；
2) sonic oscillation 5min after the acetone of 250 parts by weight is added in obtained extract M1 into step 1), it is then quiet
20min is put, removes acetone layer, mixture M 2 is made；
3) by obtained mixture M 2 in step 2) sequentially through exchange resin elution 1min and silicagel column elution
3min, the head product M3 containing phosphatidylserine is made；
4) through 35 DEG C of fermentation 8h after mixing head product M3 and the cow's milk of 100 parts by weight, the acid containing phosphatidyl choline is made
Milk A1.(phosphatidylcholine content 3.12%)
Embodiment 2
1) it is placed under conditions of temperature is 40 DEG C and extracts after the ox pancreas of 10 parts by weight is mixed with the ethanol of 250 parts by weight
10h, filter residue is filtered off, extract M1 is made；
2) sonic oscillation 15min after the acetone of 350 parts by weight is added in obtained extract M1 into step 1), it is then quiet
60min is put, removes acetone layer, mixture M 2 is made；
3) by obtained mixture M 2 in step 2) sequentially through exchange resin elution 5min and silicagel column elution
10min, the head product M3 containing phosphatidylserine is made；
4) through 40 DEG C of fermentation 15h after mixing head product M3 and the cow's milk of 120 parts by weight, the acid containing phosphatidyl choline is made
Milk A2.(phosphatidylcholine content 2.56%)
Embodiment 3
1) it is placed under conditions of temperature is 35 DEG C and extracts after the ox pancreas of 10 parts by weight is mixed with the ethanol of 200 parts by weight
8h, filter residue is filtered off, extract M1 is made；
2) sonic oscillation 10min after the acetone of 300 parts by weight is added in obtained extract M1 into step 1), it is then quiet
40min is put, removes acetone layer, mixture M 2 is made；
3) by obtained mixture M 2 in step 2) sequentially through exchange resin elution 3min and silicagel column elution
7min, the head product M3 containing phosphatidylserine is made；
4) through 38 DEG C of fermentation 12h after mixing head product M3 and the cow's milk of 110 parts by weight, the acid containing phosphatidyl choline is made
Milk A3.(phosphatidylcholine content 3.25%)
Embodiment 4
It is prepared by the preparation method according to embodiment 1, unlike, the dosage of the ethanol is 100 parts by weight, described
The dosage of acetone is 200 parts by weight, and the dosage of the cow's milk is 80 parts by weight, and the Yoghourt A4 containing phosphatidyl choline is made.(phosphatide
1.86%) phatidylcholine content is
Embodiment 5
It is prepared by the preparation method according to embodiment 2, unlike, the dosage of the ethanol is 300 parts by weight, described
The dosage of acetone is 400 parts by weight, and the dosage of the cow's milk is 150 parts by weight, and the Yoghourt A5 containing phosphatidyl choline is made.(phosphorus
1.65%) phosphatidylcholine content is
Comparative example 1
It is prepared by the preparation method according to embodiment 3, unlike, ethanol is added without, Yoghourt D1 is made.(phosphatidyl
0.23%) content of choline is
Comparative example 2
It is prepared by the preparation method according to embodiment 3, unlike, acetone is added without, Yoghourt D2 is made.(phosphatidyl
0.52%) content of choline is
Test case
A1-A5, D1 and D2 obtained above are detected into its mouthfeel by 1#, 2#, 3#, 4#, 5# respectively, obtained result such as table
Shown in 1.
Table 1
| Numbering | 1# | 2# | 3# | 4# | 5# |
| A1 | It is good in taste | It is good in taste | It is good in taste | It is good in taste | It is good in taste |
| A2 | It is good in taste | It is good in taste | Mouthfeel is general | It is good in taste | It is good in taste |
| A3 | It is good in taste | It is good in taste | It is good in taste | It is good in taste | It is good in taste |
| A4 | It is good in taste | It is good in taste | Mouthfeel is general | Mouthfeel is general | Mouthfeel is general |
| A5 | Mouthfeel is general | It is good in taste | Mouthfeel is general | Mouthfeel is general | Mouthfeel is general |
| D1 | It is good in taste | It is good in taste | It is good in taste | It is good in taste | It is good in taste |
| D2 | It is good in taste | It is good in taste | It is good in taste | It is good in taste | It is good in taste |
The preferred embodiment of the present invention described in detail above, still, the present invention are not limited in above-mentioned embodiment
Detail, in the range of the technology design of the present invention, a variety of simple variants can be carried out to technical scheme, this
A little simple variants belong to protection scope of the present invention.
It is further to note that each particular technique feature described in above-mentioned embodiment, in not lance
In the case of shield, can be combined by any suitable means, in order to avoid unnecessary repetition, the present invention to it is various can
The combination of energy no longer separately illustrates.
In addition, various embodiments of the present invention can be combined randomly, as long as it is without prejudice to originally
The thought of invention, it should equally be considered as content disclosed in this invention.
Claims (9)
1. a kind of preparation method of the Yoghourt containing phosphatidyl choline, it is characterised in that the preparation method includes：
1) extracted after ox pancreas is mixed with ethanol, filter off filter residue, extract M1 is made；
2) vibrate after adding acetone in obtained extract M1 into step 1), then stand, remove acetone layer, mixture is made
M2；
3) obtained mixture M 2 in step 2) is made sequentially through ion exchange resin and silicagel column elution and contains phosphatidyl
The head product M3 of serine；
4) through fermentation after mixing head product M3 and cow's milk, the Yoghourt containing phosphatidyl choline is made.
2. preparation method according to claim 1, wherein, relative to the ox pancreas of 10 parts by weight, the ethanol
Dosage is 100-300 parts by weight, and the dosage of the acetone is 200-400 parts by weight, and the dosage of the cow's milk is 80-150 weight
Part.
3. preparation method according to claim 2, wherein, relative to the ox pancreas of 10 parts by weight, the ethanol
Dosage is 150-250 parts by weight, and the dosage of the acetone is 250-350 parts by weight, and the dosage of the cow's milk is 100-120 weight
Part.
4. according to the preparation method described in any one in claim 1-3, wherein, extraction time is 5-10h in step 1), leaching
Temperature raising degree is 30-40 DEG C.
5. according to the preparation method described in any one in claim 1-3, wherein, vibration is sonic oscillation in step 2), and
Duration of oscillation is 5-15min, time of repose 20-60min.
6. according to the preparation method described in any one in claim 1-3, wherein, washing through ion exchange resin in step 3)
The de- time is 1-5min, and the elution time through silicagel column is 3-10min.
7. according to the preparation method described in any one in claim 1-3, wherein, fermentation temperature is 35-40 DEG C in step 4),
Fermentation time is 8-15h.
8. according to the preparation method described in any one in claim 1-3, wherein, also include adding after fermentation in step 4)
Jam is mixed.
9. the Yoghourt containing phosphatidyl choline made from the preparation method in a kind of 1-8 according to claim described in any one.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710436586.XA CN107333894A (en) | 2017-06-12 | 2017-06-12 | Yoghourt containing phosphatidyl choline and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710436586.XA CN107333894A (en) | 2017-06-12 | 2017-06-12 | Yoghourt containing phosphatidyl choline and preparation method thereof |
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| Publication Number | Publication Date |
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| CN107333894A true CN107333894A (en) | 2017-11-10 |
Family
ID=60220508
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710436586.XA Pending CN107333894A (en) | 2017-06-12 | 2017-06-12 | Yoghourt containing phosphatidyl choline and preparation method thereof |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595915A (en) * | 2009-07-04 | 2009-12-09 | 内蒙古蒙牛乳业（集团）股份有限公司 | A kind of sour milk that adds phosphatid ylcholine and preparation method thereof |
| CN102633832A (en) * | 2011-02-09 | 2012-08-15 | 北京绿色金可生物技术股份有限公司 | Method for preparing high-purity phosphatidylcholine |
| CN102924506A (en) * | 2012-11-20 | 2013-02-13 | 江南大学 | Preparation method of high-purity lecithin |
-
2017
- 2017-06-12 CN CN201710436586.XA patent/CN107333894A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101595915A (en) * | 2009-07-04 | 2009-12-09 | 内蒙古蒙牛乳业（集团）股份有限公司 | A kind of sour milk that adds phosphatid ylcholine and preparation method thereof |
| CN102633832A (en) * | 2011-02-09 | 2012-08-15 | 北京绿色金可生物技术股份有限公司 | Method for preparing high-purity phosphatidylcholine |
| CN102924506A (en) * | 2012-11-20 | 2013-02-13 | 江南大学 | Preparation method of high-purity lecithin |
Non-Patent Citations (1)
| Title |
|---|
| 刘元法等: "离子交换树脂分离磷脂酰胆碱的研究", 《中国油脂》 * |
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Application publication date: 20171110 |