CN107308492B - A kind of preparation method of antibacterial paradenlal tissue regeneration film - Google Patents
A kind of preparation method of antibacterial paradenlal tissue regeneration film Download PDFInfo
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- CN107308492B CN107308492B CN201710505771.XA CN201710505771A CN107308492B CN 107308492 B CN107308492 B CN 107308492B CN 201710505771 A CN201710505771 A CN 201710505771A CN 107308492 B CN107308492 B CN 107308492B
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/02—Inorganic materials
- A61L27/12—Phosphorus-containing materials, e.g. apatite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
- A61L27/54—Biologically active materials, e.g. therapeutic substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/106—Halogens or compounds thereof, e.g. iodine, chlorite
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/112—Phosphorus-containing compounds, e.g. phosphates, phosphonates
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/412—Tissue-regenerating or healing or proliferative agents
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
- A61L2300/602—Type of release, e.g. controlled, sustained, slow
- A61L2300/604—Biodegradation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2430/00—Materials or treatment for tissue regeneration
- A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses
Abstract
The invention discloses a kind of preparation methods of antibacterial paradenlal tissue regeneration film, prepare polycaprolactone nano fiber scaffold using electrical spinning method, utilize HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in nano fiber scaffold surface；The simple and practicable regeneration membrane simulation periodontal tissue epimatrix structure prepared of preparation method of the present invention, growing space is provided for cell；The differentiation of stem cell mineralising is induced under conditions of any growth factor is not added, so as to evade immunogenicity caused by conventional organization engineering technology must add inducible factor and be sustained the problem of difficult, fluor-apatite sustained release release fluorine ion can be with antibacterial.
Description
Technical field
The present invention relates to a kind of preparation methods of regeneration membrane, and in particular to a kind of preparation side of antibacterial paradenlal tissue regeneration film
Method available for the reparation of defective tissue, belongs to biomedical materials field.
Background technology
Periodontal disease is that the mankind are most common, one of most multiple disease, and one of the most common is exactly periodontitis, periodontal disease
Significant pathological change is the destruction of Periodontal Supporting Tissue, absorption of alveolar bone, the connective tissue destruction being attached on sclerotin and companion
There is different degrees of cementum to absorb, come off if cannot effectively treat and may result in tooth mobility；Usually, it is clinical
The upper key for obtaining paradenlal tissue regeneration is the use of screened film, and the effect of this screened film is tissue gum and epithelial cell
How the definite value in root face, and leave certain space to the regeneration of periodontal membrane tissue, the theory of this inductive bone regeneration has
Beneficial to the reconstruction of tissue junctional epithelium；
Guided tissue regeneration (guidedtissueregeneration, GTR) is widely used in periodontal surgery, is recognized
To be to cause the regenerated effective means of periodontium physiological；The membrane material for being presently used for guided tissue regeneration is divided into and can drop
Two class of membrane material and non-degradable membrane material is solved, although non-degradable membrane material has good biocompatibility and mechanical property
Can, and the effect of certain is obtained, but cannot be absorbed by tissue, it is necessary to second operation takes out, the pain of patient is added, so
It is not accepted by patients, biodegradable membrane material optionally guides defective tissue regeneration since it has, and completely may be used
The advantages that degradation, is not required second operation, facilitating operation, is attracted attention；But the biodegradable membrane material of the prior art is in power
The problems such as learning performance, degradation property, bioactivity, can not meet the requirement of bone tissue and periodontal tissue defect reparation.
The content of the invention
The shortcomings that technical problems to be solved by the invention are, overcome the prior art provides a kind of antibacterial periodontium again
The preparation method of filming, the regeneration membrane simulation periodontal tissue epimatrix structure, growing space is provided for cell；It is not adding
The differentiation of stem cell mineralising is induced under conditions of any growth factor, induction must be added so as to evade conventional organization engineering technology
The problem of immunogenicity caused by the factor and difficult sustained release, fluor-apatite sustained release release fluorine ion can be with antibacterial.
In order to solve the above technical problems, the present invention provides a kind of preparation method of antibacterial paradenlal tissue regeneration film, application
Electrical spinning method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction,
Making fluor-apatite crystallization, concrete operations are in nano fiber scaffold surface：
（（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.3-0.5M of 0.5-0.7M3)2And the KOH of 0.65-0.7M is carried out
Stirring is sufficiently mixed spare；
（2）Prepare B liquid：Select the KH of 0.3-0.5M2PO4And the KF of 0.1-0.3M be stirred be sufficiently mixed it is spare, then
By step（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 37-40 DEG C, is vibrated 10-20 minutes when small per 3-5, when 20-24 is small after harvest stent, so
Stent PBS is rinsed 1-3 times afterwards, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, use scanning electron
Micro- sem observation determines.
The technical solution that further limits of the present invention as：
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method
Stent utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in nano fiber scaffold table
Face, concrete operations are：
（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.5M of 0.5M3)2And the KOH of 0.65M is stirred and is sufficiently mixed
It is spare；
（2）Prepare B liquid：Select the KH of 0.3M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 37 DEG C, it is every 3 it is small when vibrate 10 minutes, 24 it is small when after harvest stent, then stent is used
PBS is rinsed 3 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed.
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method
Stent utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in nano fiber scaffold table
Face, concrete operations are：
1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.3M of 0.7M3)2And the KOH of 0.7M be stirred be sufficiently mixed it is standby
With；
（2）Prepare B liquid：Select the KH of 0.5M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 40 DEG C, it is every 5 it is small when vibrate 20 minutes, 20 it is small when after harvest stent, then stent is used
PBS is rinsed 1 time, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed.
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method
Stent utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in nano fiber scaffold table
Face, concrete operations are：
1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.4M of 0.6M3)2And the KOH of 0.68M be stirred be sufficiently mixed it is standby
With；
（2）Prepare B liquid：Select the KH of 0.4M2PO4And the KF of 0.2M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 39 DEG C, it is every 4 it is small when vibrate 15 minutes, 22 it is small when after harvest stent, then stent is used
PBS is rinsed 2 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed.
The beneficial effects of the invention are as follows：
The PCL nanofibers being prepared using Electrospinning are the nucleating agents in the reaction, and using Electrospun
The PCL nanofibers that technology is prepared are the prior arts, are made by prior art operation, because fiber surface potential energy is minimum,
Therefore fluor-apatite crystallization Ca5F(PO4)3Fiber nucleation is depended on, HEDTA is calcium ion chelator, controls the calcium ion in solution dense
Degree is stablized, and interruption vibration makes crystal nucleation growth evenly, controls the size of crystallization.
The antibacterial paradenlal tissue regeneration film of the present invention can simulate periodontal tissue's epimatrix structure, and life is provided for cell
Long spacing；The differentiation of stem cell mineralising can be induced under conditions of any growth factor is not added, so as to evade conventional organization
The problem of immunogenicity caused by engineering technology must add inducible factor and sustained release difficult, fluor-apatite sustained release release fluorine from
Son, can be with antibacterial.
Description of the drawings
Fig. 1 is the schematic diagram that final products scanning electron microscope is observed in the embodiment of the present invention.
Specific embodiment
HEDTA refers to N- beta-hydroxy ethyl-3-acetic acid ethylenediamines in the present invention, and PBS flushings refer to phosphate buffer；
Embodiment 1
A kind of preparation method of antibacterial paradenlal tissue regeneration film provided in this embodiment prepares using electrical spinning method and gathers oneself
Lactone nano fiber scaffold utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in receiving
Rice fibrous framework surface, concrete operations are：
（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.5M of 0.5M3)2And the KOH of 0.65M is stirred and is sufficiently mixed
It is spare；
（2）Prepare B liquid：Select the KH of 0.3M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 37 DEG C, it is every 3 it is small when vibrate 10 minutes, 24 it is small when after harvest stent, then stent is used
PBS is rinsed 3 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed as shown in Figure 1.
Embodiment 2
A kind of preparation method of antibacterial paradenlal tissue regeneration film provided in this embodiment prepares using electrical spinning method and gathers oneself
Lactone nano fiber scaffold utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in receiving
Rice fibrous framework surface, concrete operations are：
1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.3M of 0.7M3)2And the KOH of 0.7M be stirred be sufficiently mixed it is standby
With；
（2）Prepare B liquid：Select the KH of 0.5M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 40 DEG C, it is every 5 it is small when vibrate 20 minutes, 20 it is small when after harvest stent, then stent is used
PBS is rinsed 1 time, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed.
Embodiment 3
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method
Stent utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in nano fiber scaffold table
Face, concrete operations are：
1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.4M of 0.6M3)2And the KOH of 0.68M be stirred be sufficiently mixed it is standby
With；
（2）Prepare B liquid：Select the KH of 0.4M2PO4And the KF of 0.2M be stirred be sufficiently mixed it is spare, then by step
（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）The mixed liquor prepared
In, and carry out water-bath, bath temperature is 39 DEG C, it is every 4 it is small when vibrate 15 minutes, 22 it is small when after harvest stent, then stent is used
PBS is rinsed 2 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, is observed with scanning electron microscope true
It is fixed.
In addition to the implementation, the present invention can also have other embodiment.It is all to use equivalent substitution or equivalent transformation shape
Into technical solution, all fall within the present invention claims protection domain.
Claims (4)
1. a kind of preparation method of antibacterial paradenlal tissue regeneration film, it is characterised in that：Polycaprolactone is prepared using electrical spinning method
Nano fiber scaffold utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluor-apatite crystallization in Nanowire
Dimensional scaffold surface, concrete operations are：
（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.3-0.5M of 0.5-0.7M3)2And the KOH of 0.65-0.7M is stirred
It is sufficiently mixed spare；
（2）Prepare B liquid：Select the KH of 0.3-0.5M2PO4And the KF of 0.1-0.3M be stirred be sufficiently mixed it is spare, then will step
Suddenly（1）In A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared,
And carry out water-bath, bath temperature be 37-40 DEG C, per 3-5 it is small when vibrate 10-20 minutes, when 20-24 is small after harvest stent, then
Stent PBS is rinsed 1-3 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, shown with scanning electron
Micro mirror observation determines.
2. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side
Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluorine phosphorus grey
Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.5M of 0.5M3)2And the KOH of 0.65M be stirred be sufficiently mixed it is spare；
（2）Prepare B liquid：Select the KH of 0.3M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step（1）In
A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared,
And carry out water-bath, bath temperature be 37 DEG C, it is every 3 it is small when vibrate 10 minutes, 24 it is small when after harvest stent, then by stent PBS
It rinses 3 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with scanning electron microscope.
3. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side
Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluorine phosphorus grey
Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.3M of 0.7M3)2And the KOH of 0.7M be stirred be sufficiently mixed it is spare；
（2）Prepare B liquid：Select the KH of 0.5M2PO4And the KF of 0.1M be stirred be sufficiently mixed it is spare, then by step（1）In
A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared,
And carry out water-bath, bath temperature be 40 DEG C, it is every 5 it is small when vibrate 20 minutes, 20 it is small when after harvest stent, then by stent PBS
It rinses 1 time, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with scanning electron microscope.
4. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side
Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction makes fluorine phosphorus grey
Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
（1）Prepare A liquid：Select the Ca (NO of HEDTA, 0.4M of 0.6M3)2And the KOH of 0.68M be stirred be sufficiently mixed it is spare；
（2）Prepare B liquid：Select the KH of 0.4M2PO4And the KF of 0.2M be stirred be sufficiently mixed it is spare, then by step（1）In
A liquid carry out mixing abundant reaction with B liquid, it is spare；
（3）The PCL nano fiber scaffolds being prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared,
And carry out water-bath, bath temperature be 39 DEG C, it is every 4 it is small when vibrate 15 minutes, 22 it is small when after harvest stent, then by stent PBS
It rinses 2 times, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with scanning electron microscope.
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| US20180161478A1 (en) * | 2015-05-20 | 2018-06-14 | Gumusderelioglu Menemse | Barrier membrane used in periodontitis treatment and a production method thereof |
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|---|---|---|---|---|
| CN101406711A (en) * | 2008-11-04 | 2009-04-15 | 东华大学 | Method for preparing galvanic deposit calcium phosphorus mineralized layer superfine fibre bone material |
| CN101879332A (en) * | 2010-07-13 | 2010-11-10 | 北京大学 | Polyether-ether-ketone composite material containing fluorapatite and titanium dioxide and preparation method thereof |
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