CN107308492A - A kind of preparation method of antibacterial paradenlal tissue regeneration film - Google Patents

A kind of preparation method of antibacterial paradenlal tissue regeneration film Download PDF

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CN107308492A
CN107308492A CN201710505771.XA CN201710505771A CN107308492A CN 107308492 A CN107308492 A CN 107308492A CN 201710505771 A CN201710505771 A CN 201710505771A CN 107308492 A CN107308492 A CN 107308492A
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liquid
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nano fiber
antibacterial
tissue regeneration
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CN107308492B (en
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郭婷
曹罡
毛钊
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/14—Macromolecular materials
    • A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/02—Inorganic materials
    • A61L27/12—Phosphorus-containing materials, e.g. apatite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/50—Materials characterised by their function or physical properties, e.g. injectable or lubricating compositions, shape-memory materials, surface modified materials
    • A61L27/54—Biologically active materials, e.g. therapeutic substances
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
    • A61L2300/106—Halogens or compounds thereof, e.g. iodine, chlorite
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
    • A61L2300/112—Phosphorus-containing compounds, e.g. phosphates, phosphonates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
    • A61L2300/412—Tissue-regenerating or healing or proliferative agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
    • A61L2300/60—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a special physical form
    • A61L2300/602—Type of release, e.g. controlled, sustained, slow
    • A61L2300/604—Biodegradation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2400/00—Materials characterised by their function or physical properties
    • A61L2400/12—Nanosized materials, e.g. nanofibres, nanoparticles, nanowires, nanotubes; Nanostructured surfaces
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2430/00—Materials or treatment for tissue regeneration
    • A61L2430/12—Materials or treatment for tissue regeneration for dental implants or prostheses

Abstract

The invention discloses a kind of preparation method of antibacterial paradenlal tissue regeneration film, polycaprolactone nano fiber scaffold is prepared using electrical spinning method, HEDTA, Ca (NO is utilized3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in nano fiber scaffold surface；The preparation method regeneration membrane simulation periodontal tissue epimatrix structure simple and easy to apply prepared of the present invention, growing space is provided for cell；The induction stem cell mineralising differentiation under conditions of any growth factor is not added, so as to evade the problem of conventional organization engineering technology must add immunogenicity and difficult sustained release caused by inducible factor, fluor-apatite sustained release release fluorine ion can be with antibacterial.

Description

A kind of preparation method of antibacterial paradenlal tissue regeneration film
Technical field
The present invention relates to a kind of preparation method of regeneration membrane, and in particular to a kind of preparation side of antibacterial paradenlal tissue regeneration film Method, available for the reparation of defective tissue, belongs to biomedical materials field.
Background technology
Periodontal disease is one of most common, most multiple disease of the mankind, and most common of which is exactly periodontitis, periodontal disease Significant pathological change is the destruction of Periodontal Supporting Tissue, absorption of alveolar bone, the connective tissue destruction being attached on sclerotin and companion There is different degrees of cementum to absorb, come off if cannot effectively treat and may result in tooth mobility；As a rule, it is clinical The upper key for obtaining paradenlal tissue regeneration is the use of screened film, and the effect of this screened film is tissue gum and epithelial cell How the definite value in root face, and leave certain space to the regeneration of periodontal membrane tissue, the theory of this inductive bone regeneration has Beneficial to the reconstruction of tissue junctional epithelium；
Guided tissue regeneration (guidedtissueregeneration, GTR) is widely used in periodontal surgery, it is considered to be The effective means for causing periodontium physiological to regenerate；The membrane material for being presently used for guided tissue regeneration is divided into degradable membrane Material and the class of non-degradable membrane material two, although non-degradable membrane material is with good biocompatibility and mechanical property, and Obtain certain curative effect, but can not be absorbed by tissue, it is necessary to second operation takes out, add the pain of patient, thus not by Patient receives, and biodegradable membrane material has optionally guiding defective tissue regeneration, and Wholly-degradable due to it, The advantages of not needing second operation, facilitating operation, is attracted attention；But the biodegradable membrane material of prior art is in mechanical property The problems such as energy, degradation property, bioactivity, it can not meet the requirement that bone tissue and periodontal tissue defect are repaired.
The content of the invention
The technical problems to be solved by the invention be overcome the shortcoming of prior art there is provided a kind of antibacterial periodontium again The preparation method of filming, the regeneration membrane simulation periodontal tissue epimatrix structure, growing space is provided for cell；Do not adding The differentiation of stem cell mineralising is induced under conditions of any growth factor, so that induction must be added by having evaded conventional organization engineering technology Immunogenicity and the problem of sustained release difficult caused by the factor, fluor-apatite sustained release release fluorine ion can be with antibacterial.
In order to solve the above technical problems, the present invention provides a kind of preparation method of antibacterial paradenlal tissue regeneration film, application Electrical spinning method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, Fluor-apatite is set to crystallize in nano fiber scaffold surface, concrete operations are：
（（1）Prepare A liquid：From 0.5-0.7M HEDTA, 0.3-0.5M Ca (NO3)2And 0.65-0.7M KOH is stirred Mix be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.3-0.5M KH2PO4And 0.1-0.3M KF be stirred be sufficiently mixed it is standby, then will Step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 37-40 DEG C, and per 3-5 hours, vibration harvested support after 10-20 minutes, 20-24 hours, then Support is rinsed 1-3 times with PBS, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, it is aobvious with scanning electron Micro mirror observation is determined.
The technical scheme that further limits of the present invention as：
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber branch is prepared using electrical spinning method Frame, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in nano fiber scaffold surface, Concrete operations are：
（1）Prepare A liquid：From 0.5M HEDTA, 0.5M Ca (NO3)2And 0.65M KOH be stirred be sufficiently mixed it is standby With；
（2）Prepare B liquid：From 0.3M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 37 DEG C, and vibration in every 3 hours harvests support after 10 minutes, 24 hours, then by support PBS Rinse 3 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method Support, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in nano fiber scaffold table Face, concrete operations are：
1）Prepare A liquid：From 0.7M HEDTA, 0.3M Ca (NO3)2And 0.7M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.5M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 40 DEG C, and vibration in every 5 hours harvests support after 20 minutes, 20 hours, then by support PBS Rinse 1 time, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber is prepared using electrical spinning method Support, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in nano fiber scaffold table Face, concrete operations are：
1）Prepare A liquid：From 0.6M HEDTA, 0.4M Ca (NO3)2And 0.68M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.4M KH2PO4And 0.2M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 39 DEG C, and vibration in every 4 hours harvests support after 15 minutes, 22 hours, then by support PBS Rinse 2 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
The beneficial effects of the invention are as follows：
The PCL nanofibers prepared using Electrospinning are the nucleators in the reaction, and apply Electrospinning The PCL nanofibers prepared are prior arts, are made by prior art operation, because fiber surface potential energy is minimum, therefore fluorine Apatite crystal Ca5F(PO4)3Fiber nucleation is depended on, HEDTA is calcium ion chelator, the calcium ion concentration in control solution is steady Fixed, interruption vibration makes crystal nucleation growth evenly, controls the size of crystallization.
The antibacterial paradenlal tissue regeneration film of the present invention can simulate periodontal tissue's epimatrix structure, and life is provided for cell Long spacing；Induction stem cell mineralising it can break up under conditions of any growth factor is not added, so as to evade conventional organization The problem of engineering technology must add immunogenicity and difficult sustained release caused by inducible factor, fluor-apatite sustained release release fluorine from Son, can be with antibacterial.
Brief description of the drawings
The schematic diagram that Fig. 1 is observed for final products in the embodiment of the present invention with SEM.
Embodiment
HEDTA refers to N- beta-hydroxy ethyl-3-acetic acid ethylenediamines in the present invention, and PBS, which is rinsed, refers to phosphate buffer；
Embodiment 1
A kind of preparation method for antibacterial paradenlal tissue regeneration film that the present embodiment is provided, polycaprolactone is prepared using electrical spinning method Nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in Nanowire Dimensional scaffold surface, concrete operations are：
（1）Prepare A liquid：From 0.5M HEDTA, 0.5M Ca (NO3)2And 0.65M KOH be stirred be sufficiently mixed it is standby With；
（2）Prepare B liquid：From 0.3M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 37 DEG C, and vibration in every 3 hours harvests support after 10 minutes, 24 hours, then by support PBS Rinse 3 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, determined such as with SEM observation Shown in Fig. 1.
Embodiment 2
A kind of preparation method for antibacterial paradenlal tissue regeneration film that the present embodiment is provided, polycaprolactone is prepared using electrical spinning method Nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in Nanowire Dimensional scaffold surface, concrete operations are：
1）Prepare A liquid：From 0.7M HEDTA, 0.3M Ca (NO3)2And 0.7M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.5M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 40 DEG C, and vibration in every 5 hours harvests support after 20 minutes, 20 hours, then by support PBS Rinse 1 time, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
Embodiment 3
In the preparation method of foregoing antibacterial paradenlal tissue regeneration film, polycaprolactone nanofiber branch is prepared using electrical spinning method Frame, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in nano fiber scaffold surface, Concrete operations are：
1）Prepare A liquid：From 0.6M HEDTA, 0.4M Ca (NO3)2And 0.68M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.4M KH2PO4And 0.2M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 39 DEG C, and vibration in every 4 hours harvests support after 15 minutes, 22 hours, then by support PBS Rinse 2 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
In addition to the implementation, the present invention can also have other embodiment.All use equivalent substitution or equivalent transformation shape Into technical scheme, all fall within the protection domain of application claims.

Claims (4)

1. a kind of preparation method of antibacterial paradenlal tissue regeneration film, it is characterised in that：Polycaprolactone is prepared using electrical spinning method Nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluor-apatite crystallize in Nanowire Dimensional scaffold surface, concrete operations are：
（1）Prepare A liquid：From 0.5-0.7M HEDTA, 0.3-0.5M Ca (NO3)2And 0.65-0.7M KOH is stirred It is sufficiently mixed standby；
（2）Prepare B liquid：From 0.3-0.5M KH2PO4And 0.1-0.3M KF be stirred be sufficiently mixed it is standby, then will step Suddenly（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 37-40 DEG C, and per 3-5 hours, vibration harvested support after 10-20 minutes, 20-24 hours, then Support is rinsed 1-3 times with PBS, is dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, it is aobvious with scanning electron Micro mirror observation is determined.
2. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluorine phosphorus ash Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
（1）Prepare A liquid：From 0.5M HEDTA, 0.5M Ca (NO3)2And 0.65M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.3M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 37 DEG C, and vibration in every 3 hours harvests support after 10 minutes, 24 hours, then by support PBS Rinse 3 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
3. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluorine phosphorus ash Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
1）Prepare A liquid：From 0.7M HEDTA, 0.3M Ca (NO3)2And 0.7M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.5M KH2PO4And 0.1M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 40 DEG C, and vibration in every 5 hours harvests support after 20 minutes, 20 hours, then by support PBS Rinse 1 time, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
4. the preparation method of antibacterial paradenlal tissue regeneration film according to claim 1, it is characterised in that：Using Electrospun side Method prepares polycaprolactone nano fiber scaffold, utilizes HEDTA, Ca (NO3)2, KF, KOH and KH2PO4Chemical reaction, makes fluorine phosphorus ash Stone is crystallized in nano fiber scaffold surface, and concrete operations are：
（1）Prepare A liquid：From 0.6M HEDTA, 0.4M Ca (NO3)2And 0.68M KOH be stirred be sufficiently mixed it is standby；
（2）Prepare B liquid：From 0.4M KH2PO4And 0.2M KF be stirred be sufficiently mixed it is standby, then by step（1）In A liquid and B liquid carry out mixing abundant reaction, it is standby；
（3）The PCL nano fiber scaffolds prepared using electrical spinning method are immersed into step（2）In the mixed liquor prepared, Water-filling of going forward side by side is bathed, and bath temperature is 39 DEG C, and vibration in every 4 hours harvests support after 15 minutes, 22 hours, then by support PBS Rinse 2 times, be dried in vacuo can obtain antibacterial paradenlal tissue regeneration film recently, observed and determined with SEM.
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Publication number Priority date Publication date Assignee Title
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