CN107308281A - It is a kind of to treat medicine of orthopaedic disease and its preparation method and application - Google Patents
It is a kind of to treat medicine of orthopaedic disease and its preparation method and application Download PDFInfo
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- CN107308281A CN107308281A CN201710534327.0A CN201710534327A CN107308281A CN 107308281 A CN107308281 A CN 107308281A CN 201710534327 A CN201710534327 A CN 201710534327A CN 107308281 A CN107308281 A CN 107308281A
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/09—Lichens
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/41—Crassulaceae (Stonecrop family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/57—Magnoliaceae (Magnolia family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/67—Piperaceae (Pepper family), e.g. Jamaican pepper or kava
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/79—Schisandraceae (Schisandra family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/899—Poaceae or Gramineae (Grass family), e.g. bamboo, corn or sugar cane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Abstract
The invention discloses a kind of medicine for treating orthopaedic disease and its preparation method and application, the medicine is made up of the raw material below according to parts by weight：15 25 parts of blackspiked lovegrass herb with root, 11 19 parts of rhodiola root, fragrant 15 parts of blood, 8 16 parts of the Bi roots of grass, 37 parts of green alectoria filament.Rhodiola root and ground blood face powder is broken, using ethanol as entrainer, use CO2Means of supercritical extraction, is made supercritical extract；Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, the dissolving of 45% ethanol is added, microwave treatment 3 times is concentrated under reduced pressure into medicinal extract, obtains mixture B；After mixture A is mixed with mixture B, deionized water heating stirring, ultrasonically treated, centrifugation are added, takes filtrate, stirring to be produced to dry.It is fast that the present invention is used to treating the orthopaedic disease clinical symptom disappearances such as waist dish outstanding, sciatica, rheumatism class ostalgia, has no toxic side effect, cure rate height, non-relapse after healing, treats both principal and secondary aspect of disease, instant is safe, and production cost is low, suitable for industrialized production.
Description
Technical field
It is specifically a kind of to treat medicine of orthopaedic disease and preparation method thereof and answer the present invention relates to pharmaceutical technology field
With.
Background technology
At present, rheumatoid arthritis, rheumatism, rheumatic arthritis, Sjogren syndrome, cervical spondylopathy, osteoarthritis, strong
A series of rheumatism such as straightforward rachitis, rheumatic fever, orthopaedic disease are China's common disease and frequently-occurring disease, and serious influence people are just
Often live and healthy.Treatment orthopaedics difficult and complicated cases always are the problem of medical profession, and the operation risk of doctor trained in Western medicine is big, expense
Height, Chinese medicine poor taste, program is cumbersome, slowly effect.Allergy is easily sent out in traditional plaster external application, along with leaded composition is long-term with to people
Skin body all be harmful to, oral western medicine to stomach and intestine, liver kidney have stimulation and damage.Rheumatism pure using drug therapy or
Massage, acupuncture, massage can only respite pain symptom, do not reach therapeutic purposes, medicine has one with many to internal organs, organ
Fixed influence, it is impossible to tackle the problem at its root.Treatment by Chinese herbs also has that prescription is numerous and diverse, cost is high in various degree, course for the treatment of length,
Side effect is big, more after recurrent exerbation the shortcomings of.And operative treatment, high cost is risky, and patient suffering is big, is not easily accepted by.
The content of the invention
It is an object of the invention to provide a kind of medicine for treating orthopaedic disease and its preparation method and application, with solution
State the problem of being proposed in background technology.
To achieve the above object, the present invention provides following technical scheme：
A kind of medicine for treating orthopaedic disease, is made up of the raw material below according to parts by weight：15-25 parts of blackspiked lovegrass herb with root, red scape
Its 11-19 parts, 1-5 parts of blood perfume, 8-16 parts of the Bi roots of grass, 3-7 parts of green alectoria filament.
It is used as further scheme of the invention：The medicine of the treatment orthopaedic disease, by below according to the raw material of parts by weight
Composition：18-22 parts of blackspiked lovegrass herb with root, 13-17 parts of rhodiola root, 2-4 parts of blood perfume, 10-14 parts of the Bi roots of grass, 4-6 parts of green alectoria filament.
It is used as further scheme of the invention：The medicine of the treatment orthopaedic disease, by below according to the raw material of parts by weight
Composition：20 parts of blackspiked lovegrass herb with root, 15 parts of rhodiola root, fragrant 3 parts of blood, 12 parts of the Bi roots of grass, 5 parts of green alectoria filament.
A kind of preparation method for the medicine for treating orthopaedic disease, comprises the following steps：
1) rhodiola root and ground blood face powder is broken, the ethanol using 4.3 times of the two quality uses CO as entrainer2Means of supercritical extraction,
Supercritical extract is made；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h, extraction time
4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure 3MPa；Face super
Boundary's extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the eluent low temperature drying of collection
Produce mixture A；
2) blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, added at the ethanol of volumetric concentration 45% dissolving of 12 times of quality, microwave
Reason 3 times, each 12min merges extract solution, is concentrated under reduced pressure into medicinal extract, obtains mixture B；
3) after mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, is stirred
Afterwards, 70-75 DEG C and at such a temperature heating stirring 1-1.2h are warming up to, then ultrasonically treated 10-15min, centrifuge, take filtrate,
Stirred again at a temperature of 70-75 DEG C to dry and produce medicine.
It is used as further scheme of the invention：Step 1) in, by rhodiola root with ground blood face powder it is broken, cross 100-150 mesh sieves.
It is used as further scheme of the invention：Step 2) in, blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 100-150 mesh is crossed
Sieve.
It is used as further scheme of the invention：Step 2) in, microwave power is 1000W.
It is used as further scheme of the invention：Step 3) in, ultrasonic power is 700W.
It is used as further scheme of the invention：Step 3) in, centrifugal process is to centrifuge 5- under 1000r/min rotating speed
10min。
Application of the medicine in treatment orthopaedic disease medicine is prepared.
Blackspiked lovegrass herb with root is clearing and activating the channels and collaterals, damp dispelling and pain relieving, cures mainly pain in rheumatoid joints.
Rhodiola root has functions that tonifying Qi clearing lung-heat, intelligence development nourishing heart, controlling of bleeding with astringents, eliminating stasis to subdue swelling.Cure mainly deficiency of vital energy and physically weak, fear after being ill
Cold, shortness of breath and fatigue, cough with lung heat, spitting of blood, leukorrhea diarrhoea, traumatic injury etc..
Ground blood perfume has dispelling wind and eliminating dampness, promoting qi circulation and relieving pain, the effect of stimulating the circulation of the blood and cause the muscles and joints to relax.It is usually used in arthralgia pain due to rheumatism, has a stomachache, stomachache,
Dysmenorrhoea, postpartum abdominal pain, traumatic injury, chronic lumbocrural pain.
Bi roots of grass warming spleen and stomach for dispelling cold, lower gas analgesic.For coldness and pain in the epigastrium, vomiting, diarrhea, cold obstruction causing qi stagnation, chest impediment and cardialgia is had a headache,
Toothache.
Green alectoria filament curing rheumatism, hemostasis and pain-relieving, regulating menstruation and activating blood, tranquillizing the mind by relieving convulsion, strengthening the spleen and stomach.Dispelling, collateral-activating, tonifying kidney and strengthening yang.Cure mainly
Pain in low back and legs, traumatism and bleeding, irregular menstruation, dizziness, hemiplegia, lumbago due to the kidney deficiency, impotence, epilepsy.
Compared with prior art, the beneficial effects of the invention are as follows：
The present invention is fragrant fast through medicine effect made from above-mentioned technique using blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament, rhodiola root and ground blood
Speed, nontoxic secondary work fast for treating the orthopaedic disease clinical symptom disappearances such as waist dish outstanding, sciatica, rheumatism class ostalgia
With；Short treating period, pain is small, and stability is good, and cost is low, cure rate height, non-relapse after healing, treats both principal and secondary aspect of disease, fast treating loins
The orthopaedic diseases such as disk protrusion, sciatica, rheumatism class ostalgia.Preparation technology of the present invention is simple, type of feed is few, instant
Safety, production cost is low, suitable for industrialized production.
Embodiment
Below in conjunction with the embodiment of the present invention, the technical scheme in the embodiment of the present invention is clearly and completely described,
Obviously, described embodiment is only a part of embodiment of the invention, rather than whole embodiments.Based in the present invention
Embodiment, the every other embodiment that those of ordinary skill in the art are obtained under the premise of creative work is not made, all
Belong to the scope of protection of the invention.
Embodiment 1
In the embodiment of the present invention, a kind of medicine for treating orthopaedic disease is made up of following raw material：Blackspiked lovegrass herb with root 15kg, red scape
Its 11kg, blood perfume 1kg, Bi roots of grass 8kg, green alectoria filament 3kg.
By rhodiola root and ground blood face powder it is broken, cross 100 mesh sieves, the ethanol using 4.3 times of the two quality uses CO as entrainer2It is super
Critical extraction, is made supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h,
Extraction time 4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure
3MPa；Supercritical extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the elution of collection
Liquid low temperature drying produces mixture A.Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 100 mesh sieves are crossed, the volume for adding 12 times of quality is dense
Spend 45% ethanol to dissolve, microwave treatment 3 times, microwave power is 1000W, each 12min, merge extract solution, be concentrated under reduced pressure into leaching
Cream, obtains mixture B.After mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, stirring is equal
After even, 70 DEG C and at such a temperature heating stirring 1h are warming up to, then ultrasonically treated 10min, ultrasonic power is 700W,
5min is centrifuged under 1000r/min rotating speed, filtrate is taken, then is stirred at a temperature of 70 DEG C to dry and produces medicine.
Embodiment 2
In the embodiment of the present invention, a kind of medicine for treating orthopaedic disease is made up of following raw material：Blackspiked lovegrass herb with root 25kg, red scape
Its 19kg, blood perfume 5kg, Bi roots of grass 16kg, green alectoria filament 7kg.
By rhodiola root and ground blood face powder it is broken, cross 150 mesh sieves, the ethanol using 4.3 times of the two quality uses CO as entrainer2It is super
Critical extraction, is made supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h,
Extraction time 4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure
3MPa；Supercritical extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the elution of collection
Liquid low temperature drying produces mixture A.Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 150 mesh sieves are crossed, the volume for adding 12 times of quality is dense
Spend 45% ethanol to dissolve, microwave treatment 3 times, microwave power is 1000W, each 12min, merge extract solution, be concentrated under reduced pressure into leaching
Cream, obtains mixture B.After mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, stirring is equal
After even, 75 DEG C and at such a temperature heating stirring 1.2h are warming up to, then ultrasonically treated 15min, ultrasonic power is 700W,
10min is centrifuged under 1000r/min rotating speed, filtrate is taken, then is stirred at a temperature of 75 DEG C to dry and produces medicine.
Embodiment 3
In the embodiment of the present invention, a kind of medicine for treating orthopaedic disease is made up of following raw material：Blackspiked lovegrass herb with root 18kg, red scape
Its 13kg, blood perfume 2kg, Bi roots of grass 10kg, green alectoria filament 4kg.
By rhodiola root and ground blood face powder it is broken, cross 120 mesh sieves, the ethanol using 4.3 times of the two quality uses CO as entrainer2It is super
Critical extraction, is made supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h,
Extraction time 4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure
3MPa；Supercritical extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the elution of collection
Liquid low temperature drying produces mixture A.Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 120 mesh sieves are crossed, the volume for adding 12 times of quality is dense
Spend 45% ethanol to dissolve, microwave treatment 3 times, microwave power is 1000W, each 12min, merge extract solution, be concentrated under reduced pressure into leaching
Cream, obtains mixture B.After mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, stirring is equal
After even, 72 DEG C and at such a temperature heating stirring 1.1h are warming up to, then ultrasonically treated 12min, ultrasonic power is 700W,
8min is centrifuged under 1000r/min rotating speed, filtrate is taken, then is stirred at a temperature of 72 DEG C to dry and produces medicine.
Embodiment 4
In the embodiment of the present invention, a kind of medicine for treating orthopaedic disease is made up of following raw material：Blackspiked lovegrass herb with root 22kg, red scape
Its 17kg, blood perfume 4kg, Bi roots of grass 14kg, green alectoria filament 6kg.
By rhodiola root and ground blood face powder it is broken, cross 120 mesh sieves, the ethanol using 4.3 times of the two quality uses CO as entrainer2It is super
Critical extraction, is made supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h,
Extraction time 4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure
3MPa；Supercritical extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the elution of collection
Liquid low temperature drying produces mixture A.Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 120 mesh sieves are crossed, the volume for adding 12 times of quality is dense
Spend 45% ethanol to dissolve, microwave treatment 3 times, microwave power is 1000W, each 12min, merge extract solution, be concentrated under reduced pressure into leaching
Cream, obtains mixture B.After mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, stirring is equal
After even, 72 DEG C and at such a temperature heating stirring 1.1h are warming up to, then ultrasonically treated 12min, ultrasonic power is 700W,
8min is centrifuged under 1000r/min rotating speed, filtrate is taken, then is stirred at a temperature of 72 DEG C to dry and produces medicine.
Embodiment 5
In the embodiment of the present invention, a kind of medicine for treating orthopaedic disease is made up of following raw material：Blackspiked lovegrass herb with root 20kg, red scape
Its 15kg, blood perfume 3kg, Bi roots of grass 12kg, green alectoria filament 5kg.
By rhodiola root and ground blood face powder it is broken, cross 120 mesh sieves, the ethanol using 4.3 times of the two quality uses CO as entrainer2It is super
Critical extraction, is made supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h,
Extraction time 4.8h；First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure
3MPa；Supercritical extract is adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the elution of collection
Liquid low temperature drying produces mixture A.Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, 120 mesh sieves are crossed, the volume for adding 12 times of quality is dense
Spend 45% ethanol to dissolve, microwave treatment 3 times, microwave power is 1000W, each 12min, merge extract solution, be concentrated under reduced pressure into leaching
Cream, obtains mixture B.After mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, stirring is equal
After even, 72 DEG C and at such a temperature heating stirring 1.1h are warming up to, then ultrasonically treated 12min, ultrasonic power is 700W,
8min is centrifuged under 1000r/min rotating speed, filtrate is taken, then is stirred at a temperature of 72 DEG C to dry and produces medicine.
Comparative example 1
In addition to blackspiked lovegrass herb with root and ground blood perfume is not contained, it is formulated and preparation process is consistent with embodiment 5.
Comparative example 2
Only fragrant containing blackspiked lovegrass herb with root and ground blood, its preparation process is consistent with embodiment 5.
Comparative example 3
Formula is consistent with embodiment 5, the difference is that being added water to cook after each raw material is mixed when preparing, filtering to take filtrate, baking
It is dry to produce medicine.
In order to prove the therapeutic effect of medicine of the present invention, following experiments are carried out：
First, toxicity test
The medicine of the present invention is that one kind can treat caput femoris necrosis, osteoproliferation, various traumatic injuries and arthritic
Specific medicament, now by taking medicine made from embodiment 5 as an example, carries out studies on acute toxicity.
1st, experiment material
1.1 medicine：The made medicine of embodiment 5.
1.2 animal：Mouse, Kunming kind, 18-22 grams of body weight, male and female are all used, real purchased from Shandong Pharmaceutical Group Lu Yuan branch companies
Test animal center.
2nd, method and result
18-22 grams of kunming mice of body weight 10 is taken, male and female half and half, 33mg/10 grams of gavage this product is normal to raise, and sees
Examine seven days.
As a result：Animal is fed, drinking-water, fur, and without exception, the increased weight such as four limbs activity and behavior, dissection naked eyes are seen
Wipe, main organs no abnormality seen.
3rd, brief summary and discussion
33mg/10 grams of a gavage of the invention, see to wipe has any toxic reaction less than animal to this, due to by preparation and filling
Note approach consumption is limited, and gavage can not obtain median lethal dose of the mouse to raw bone ball, can only substantially calculate gavage of mouse
The maximal tolerance dose (MTO) of raw bone ball is more than 33mg/10g.This dose is calculated by kg body weight, equivalent to people's dose
More than 100 times, the medicine of embodiment 5 safe without toxic side effect in the case of oral.
Obtained medicine progress toxicity test is also without exception in other scope of the invention.
2nd, clinical trial
To show the therapeutic effect of medicine of the present invention, it is selected in 800 waist dish outstandings, sciatica, rheumatism class ostalgia
Etc. the patient of orthopaedic disease, be randomly divided into 8 groups, every group 100, respectively treat 1 group, 2 groups for the treatment of, 3 groups for the treatment of, 4 groups for the treatment of,
5 groups and 1 group of control, 2 groups of control, 3 groups of control are treated, each group state of an illness is basically identical.Treatment 1-5 groups take embodiment 1- respectively
5 medicine, control 1-3 groups take comparative example 1-3 medicine, usage and dosage respectively：Three times a day, daily 1.5g.Face through system
Bed observation carries out statistical analysis and is summarized as follows：
First, clinical manifestation
1st, CT, X photograph film making hospital diagnosis.
2nd, related assays are reported.
3rd, pain and tenderness is ached：Pain and there is direct or indirect tenderness at joint, cervical vertebra.
2nd, curative effect determinate standard
Clinical cure：Various symptoms disappear.
It is effective：Finger possesses with the next item down person, 1, sensory disturbance largely recover normal；2nd, flesh holds, muscular strength part is recovered just
Often；3rd, vegetative nerve functional disturbance largely recovers normal；4th, pathologic reflex disappears.
Effectively：Finger possesses with the next item down person, 1, subjective symptoms part take a turn for the better or feel that suffering limb is strong, flexibly；2nd, barrier is felt
Part is hindered to take a turn for the better；3rd, vegetative nerve functional disturbance part takes a turn for the better.
It is invalid：Various symptoms and sign are without change.
3rd, the treatment results of medication one month are as shown in table 1.
Table 1
Clinical therapeutic efficacy shows that 1-5 of the embodiment of the present invention is better than control 1-3 groups, the medicine that 1-5 of the embodiment of the present invention is provided
The cure rate of thing treatment orthopaedic disease is more than 83%, and total effective rate is more than 99%；Wherein embodiment 3-5 is preferable, cure rate
More than 94%, embodiment 5 is optimal, and cure rate is up to 100%.Embodiment 5 is compared with comparative example 1-3, it is known that, the present invention be
There is above-mentioned effect using the preparation technology of the present invention under the synergy of each raw material.
Model case：
Case 1：Mr. Wang, male, 58 years old, suffer from rheumatic arthritis, osteoproliferation for many years, medication was not felt any better for many years.Take
4 drug therapy of the embodiment of the present invention 1 month, has fully recovered to examination in hospital, has not violated so far.
Case 2：Song, female, 47 years old, the patient suffered from lumbar spine disc herniation protrusion, takes action constant, Multiple Hospitalization treatment, it is impossible to cure,
Take 3 drug therapy of the embodiment of the present invention 1 month, can freedom of movement, hospital check waist fully recovered.
Case 3：Lee, man, 61 years old, suffers from sciatica, takes 5 drug therapy of the embodiment of the present invention 1 month, to hospital
Inspection has been fully recovered, and is not violated so far.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be realized in other specific forms.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit is required rather than described above is limited, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art
It may be appreciated other embodiment.
Claims (10)
1. a kind of medicine for treating orthopaedic disease, it is characterised in that be made up of the raw material below according to parts by weight：Blackspiked lovegrass herb with root 15-
25 parts, 11-19 parts of rhodiola root, 1-5 parts of blood perfume, 8-16 parts of the Bi roots of grass, 3-7 parts of green alectoria filament.
2. the medicine for the treatment of orthopaedic disease according to claim 1, it is characterised in that by below according to the raw material of parts by weight
Composition：18-22 parts of blackspiked lovegrass herb with root, 13-17 parts of rhodiola root, 2-4 parts of blood perfume, 10-14 parts of the Bi roots of grass, 4-6 parts of green alectoria filament.
3. the medicine for the treatment of orthopaedic disease according to claim 1, it is characterised in that by below according to the raw material of parts by weight
Composition：20 parts of blackspiked lovegrass herb with root, 15 parts of rhodiola root, fragrant 3 parts of blood, 12 parts of the Bi roots of grass, 5 parts of green alectoria filament.
4. a kind of preparation method of the medicine for the treatment of orthopaedic disease as described in claim 1-3 is any, it is characterised in that including
Following steps：
1) rhodiola root and ground blood face powder is broken, the ethanol using 4.3 times of the two quality uses CO as entrainer2Means of supercritical extraction, is made
Supercritical extract；Design parameter is：72 DEG C of extraction kettle temperature, extracting pressure 12MPa, flow velocity 7.5L/h, extraction time 4.8h；
First separating still：55 DEG C of temperature, separating pressure 6MPa；Second separating still：48 DEG C of temperature, separating pressure 3MPa；By overcritical extraction
Thing is taken to be adsorbed by macroporous absorbent resin, with the ethanol elution that volumetric concentration is 25%, the eluent low temperature drying of collection is produced
Mixture A；
2) blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, adds the ethanol of volumetric concentration 45% dissolving of 12 times of quality, microwave treatment 3
Secondary, each 12min merges extract solution, is concentrated under reduced pressure into medicinal extract, obtains mixture B；
3) after mixture A is mixed with mixture B, the deionized water of the two 9.8 times of quality is then added, after stirring, is risen
Temperature to 70-75 DEG C and at such a temperature heating stirring 1-1.2h, then ultrasonically treated 10-15min, is centrifuged, and takes filtrate, then
Stirred at a temperature of 70-75 DEG C to dry and produce medicine.
5. the preparation method of the medicine for the treatment of orthopaedic disease according to claim 4, it is characterised in that step 1) in, will
Rhodiola root and ground blood face powder are broken, cross 100-150 mesh sieves.
6. the preparation method of the medicine for the treatment of orthopaedic disease according to claim 4, it is characterised in that step 2) in, will
Blackspiked lovegrass herb with root, the Bi roots of grass, green alectoria filament are crushed, cross 100-150 mesh sieves.
7. the preparation method of the medicine for the treatment of orthopaedic disease according to claim 4, it is characterised in that step 2) in, it is micro-
Wave power is 1000W.
8. the preparation method of the medicine for the treatment of orthopaedic disease according to claim 4, it is characterised in that step 3) in, surpass
Acoustical power is 700W.
9. the preparation method of the medicine for the treatment of orthopaedic disease according to claim 4, it is characterised in that step 3) in, from
Heart process is to centrifuge 5-10min under 1000r/min rotating speed.
10. application of the medicine in treatment orthopaedic disease medicine is prepared as described in claim 1-3 is any.
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Cited By (1)
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Application publication date: 20171103 |