CN107307429B - Film coating premix of probiotic preparation and preparation method and application thereof - Google Patents
Film coating premix of probiotic preparation and preparation method and application thereof Download PDFInfo
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- CN107307429B CN107307429B CN201710538104.1A CN201710538104A CN107307429B CN 107307429 B CN107307429 B CN 107307429B CN 201710538104 A CN201710538104 A CN 201710538104A CN 107307429 B CN107307429 B CN 107307429B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
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Abstract
The invention relates to the technical field of film coating premixes of probiotic preparations, in particular to a film coating premix of probiotic preparations and a preparation method and application thereof. A film coating premix of a probiotic preparation comprises the following components in percentage by mass: 50-70% of methacrylic acid and ethyl acrylate copolymer; 20-30% of talcum powder; 6-8% of a plasticizer; 2-12% of an anti-sticking agent; 0.05-0.1% of dimethyl silicone oil; the invention has the beneficial effects that: the enteric-coated film coating technology is introduced to the production and processing of the probiotic preparation, so that the probiotics are isolated from the external environment and are free from the influence of oxygen, moisture, carbon dioxide, light, temperature change and the like in the air, the stability of the probiotic preparation is ensured, and the problems in the prior art are economically and effectively solved.
Description
Technical Field
The invention relates to the technical field of film coating premixes of probiotic preparations, in particular to a film coating premix of probiotic preparations and a preparation method and application thereof.
Background
Probiotics is a kind of active microorganisms beneficial to a host, and is a general term for active beneficial microorganisms which are planted in intestinal tracts and reproductive systems of human bodies and animal bodies and can generate definite health efficacy so as to improve the microbial ecological balance of the host and play beneficial roles. The beneficial bacteria or fungi in human bodies and animal bodies are mainly as follows: clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes, saccharomycetes and the like. With the growing demand of the animal feed market for high quality animal products, probiotic feed additives play an important role in providing balanced nutrition for livestock and poultry and improving digestibility.
"by bacterial type (lactobacilli, streptococcus suis, thermophiles and bifidobacteria) issued by the analytical agencies market and market; livestock and poultry (cattle, poultry, swine and aquatic) species; animal feed market reports of the form (dry, wet), function (yield, immunity, and productivity) and regional classifications "show that in 2016-.
The products with the strongest research functions in the world at present mainly comprise the composite active probiotics consisting of various microorganisms, and are widely applied to the fields of bioengineering, industry and agriculture, food safety and life health. Can improve animal gastrointestinal environment, inhibit harmful bacteria growth, effectively decompose anti-nutritional factor in feed, release nutrient substances, promote digestion and absorption, improve feed utilization rate, and improve animal production performance. However, probiotic preparations are based on the fact that the arrival of the probiotic itself in the intestine and stomach is always a viable organism. And the amount of probiotics reaching the intestinal tract is greatly reduced due to the extremely acidic and cholate environment in the stomach of the animal body. Therefore, the activity of the probiotics in the probiotic product is taken as an important index for inspecting the quality of the product. Besides the need of gastrointestinal tract examination for in vivo colonization, the probiotic products must be considered to maintain activity during storage, transportation and processing.
To achieve the above purpose, the current technologies mainly include spray drying, freeze drying and embedding technologies. However, these techniques have some significant disadvantages, such as the effect of the spray drying technique on the microbial activity due to high temperature and dehydration during the processing of the probiotic preparation. The embedding technology using sodium alginate as the main wall material greatly reduces the survival rate of probiotics after 2 hours in gastric juice due to the limited acid resistance of the microcapsule prepared by the sodium alginate.
Disclosure of Invention
The invention aims to solve the problems and provides a film coating premix of a probiotic preparation as well as a preparation method and application thereof.
The specific technical scheme of the invention is as follows:
a film coating premix of a probiotic preparation comprises the following components in percentage by mass:
50-70% of methacrylic acid and ethyl acrylate copolymer;
20-30% of talcum powder;
6-8% of a plasticizer;
2-12% of an anti-sticking agent;
0.05-0.1% of dimethyl silicone oil.
Further, the film coating premix of the probiotic preparation comprises the following components in percentage by mass:
69.9% of methacrylic acid and ethyl acrylate copolymer;
20% of talcum powder;
8% of a plasticizer;
2% of an anti-sticking agent;
0.1 percent of dimethyl silicone oil.
Further, the ratio of the methacrylic acid and the ethyl acrylate copolymer is as follows in percentage by mass: A1-A2.
Further, the ratio of the methacrylic acid and the ethyl acrylate copolymer is 1: 1.
further, the plasticizer is one or a mixture of any several of propylene glycol, polyethylene glycol, triethyl citrate and triacetin.
Further, the plasticizer is triethyl citrate.
Further, the anti-sticking agent is one or a mixture of more of stearic acid, silicon dioxide and micropowder silica gel in any proportion.
Further, the preparation method of the film coating premix of the probiotic preparation comprises the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to the mass percentage, and uniformly stirring to obtain a mixed solid material;
and weighing triethyl citrate according to the mass percentage, adding the dimethyl silicone oil into the mixed solid material, and uniformly stirring to obtain the film coating premix of the probiotic preparation.
Further, the application of the film coating premix of the probiotic preparation is used for coating the probiotic preparation.
Compared with the prior art, the invention has the following beneficial effects:
(1) the film coating premix of the probiotic preparation disclosed by the invention is used for coating the probiotic preparation, so that the probiotic preparation is isolated from the external environment and is free from the influence of oxygen, moisture, carbon dioxide, light, temperature change and the like in the air, and the stability of the probiotic preparation is ensured.
(2) The film coating premix of the probiotic preparation mainly takes water-soluble enteric raw material methacrylic acid and ethyl acrylate copolymer as a film forming agent, ethanol or other organic solvents are not needed in the whole processing link, and in the whole coating process, the surface temperature of thalli can be normally coated only by maintaining the temperature at 35-36 ℃, so that the influence of the processing link of the probiotic preparation on the activity of the thalli is greatly avoided; meanwhile, the coat film formed by using the methacrylic acid and ethyl acrylate copolymer as a main film forming material has strong acid resistance, and can prevent the probiotic preparation from being corroded by gastric acid in a gastric juice environment for 2 hours.
(3) Compared with the uncoated probiotic preparation in the prior art, the film coating premix of the probiotic preparation completely loses activity when an experiment is carried out for 10 days, and the probiotic preparation coated by the film coating technology has the strongest capability of being free from the influence of temperature and humidity; meanwhile, the activity of the probiotic preparation after film coating after artificial gastric juice test on day 15 is far greater than that of the probiotic preparation by microcapsule technology.
(4) The film coating premix of the probiotic preparation is simpler in operation process and easier to popularize when being manufactured and used. Can be widely applied to the processing and production of probiotic preparations such as food, health products and the like.
Detailed Description
The present invention will now be described more fully hereinafter with reference to the accompanying examples, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The specific meanings of the above terms in the present invention can be understood in specific cases by those skilled in the art.
A film coating premix of a probiotic preparation comprises the following components in percentage by mass:
50-70% of methacrylic acid and ethyl acrylate copolymer;
20-30% of talcum powder;
6-8% of a plasticizer;
2-12% of an anti-sticking agent;
0.05-0.1% of dimethyl silicone oil.
The film coating technology is characterized in that the differences of physicochemical properties of various inert high polymer materials with strong chemical stability and molecular arrangement and structural characteristics thereof are utilized, and the film coating premix is formed by reasonably proportioning and adding other auxiliary materials under mild processing conditions without changing the chemical properties of the materials, activating specific genes of the high polymer materials, activating surface activity and changing the arrangement mode of original substance molecules.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, as follows: A1-A2.
The water-soluble enteric raw material methacrylic acid and ethyl acrylate copolymer is used as a film forming agent, ethanol or other organic solvents are not needed in the whole processing link, in addition, the surface temperature of the thalli can be normally coated only by maintaining the temperature at 35-36 ℃ in the whole coating process, the influence of the processing link of the probiotic preparation on the activity of the thalli is greatly avoided, meanwhile, the coating film formed by using the methacrylic acid and ethyl acrylate copolymer as a main film forming material has strong acid resistance, and the probiotic preparation can be prevented from being corroded by gastric acid in a gastric juice environment for 2 hours.
The film forming material selected by the product is methacrylic acid and ethyl acrylate copolymer, and the proportion is 1: 1, screening the dosage of the compound, and designing an experiment as follows:
adding a certain amount of purified water into a beaker, slowly and uniformly adding the materials in the formula, and fully stirring (stirring speed of 1000-1500 rpm) for 45 minutes to prepare a suspension for film coating. The solids content is about 10%. And respectively pouring a proper amount of the suspension onto a smooth glass plate, pushing the suspension into a layer of solution with uniform thickness by using a wet film preparation device, and drying or naturally drying the solution in an oven at the temperature of 40-50 ℃ to obtain the film.
The results of the experiment are shown in the following table:
it can be seen that too low a proportion of methacrylic acid and ethyl acrylate copolymer results in a coating film with significantly insufficient film forming properties, but too high a proportion results in a coating film with insufficient elasticity and breaking tendency. Therefore, the using amount of the methacrylic acid and ethyl acrylate copolymer is determined to be 50-70%.
In this embodiment, the plasticizer is one or a mixture of any several of propylene glycol, polyethylene glycol, triethyl citrate, and triacetin.
After the temperature of some film coating powders is reduced, the physical properties are changed, the mobility of macromolecules of the film coating powders is reduced, so that the film coating layers are hard and brittle and lack necessary flexibility, and the film coating powders are easy to break;
the plasticizer can increase the plasticity of the coating material so as to improve the film coating operability, the flexibility of the coating film and the barrier effect of the film. But it is preferable that triethyl citrate is used as the plasticizer in view of affecting the taste of the probiotic preparation.
In this example, the dosage of triethyl citrate is taken as an example of a plasticizer, and the following design is made:
weighing the methacrylic acid-ethyl acrylate copolymer and the triethyl citrate of the above formulas, placing the mixture in a small mixer, starting equipment to shear, stirring and mixing for 10 minutes, and enabling the mixture to be uniform.
Adding a certain amount of purified water into a beaker, slowly and uniformly adding the materials in the formula, and fully stirring (stirring speed of 1000-1500 rpm) for 45 minutes to prepare a suspension for film coating.
The results are shown in the following table:
and (4) conclusion: from the above results, it is known that free film formation is not easy to occur with the increase of the amount of triethyl citrate, the film forming properties of the samples of B-1, B-2, B-3 and B-4 batches are the best, and the elasticity of the coating film of the sample of B-1 batch is not improved significantly. Thus, the amount of triethyl citrate added in the batches B-2, B-3 and B-4 was determined to be the amount of plasticizer used in the present invention.
In this embodiment, the anti-sticking agent is one or a mixture of several of stearic acid, silica and silica micropowder in any proportion.
The anti-sticking agent is mainly used for coating a sticky reagent, so that the phenomenon of adhesion in the coating process is avoided, and the anti-sticking agent can improve the phenomenon.
In the present embodiment, the proportions of talc powder, anti-sticking agent and simethicone as the filling agent in the formulation are the conventional proportions of the film coating premix.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to the mass percentage, and uniformly stirring to obtain a mixed solid material;
and weighing triethyl citrate according to the mass percentage, adding the dimethyl silicone oil into the mixed solid material, and uniformly stirring to obtain the film coating premix of the probiotic preparation.
This embodiment, earlier with solid material mixing, add liquid material and mix, make the mixture more even, when avoiding disposable mixing, the material that liquid and wherein certain material part mixed earlier caused distributes phenomenons such as inhomogeneous.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
When the probiotics are coated, the probiotics preparation is placed in a fluidized bed for test, the fluidized bed is started, the frequency of an air exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the temperature of the material to be 35-36 ℃ to coat the probiotic particle preparation.
The weight increment of the coating of the probiotic particles is controlled to be 20-30%, so that the probiotic particle preparation has no cracking and discoloration phenomenon in gastric juice with the pH of 1.0 for 2 hours.
The probiotic preparation can improve the gastrointestinal environment of animals, inhibit the growth of harmful bacteria, effectively decompose anti-nutritional factors in the feed, release nutrients, ensure that the anti-nutritional factors can be fully digested and absorbed, improve the utilization rate of the feed and improve the production performance of the animals; after the coating is coated by the film, the film can be prevented from being influenced by oxygen, moisture, carbon dioxide, light, temperature change and the like in the air, so that the stability of the probiotic preparation is ensured, and the probiotic preparation is prevented from being corroded by gastric acid in a gastric acid environment for 2 hours.
Example 1
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 52%;
30% of talcum powder;
6% of a plasticizer;
11.95 percent of anti-sticking agent;
0.05 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this embodiment, the plasticizer is triethyl citrate.
In this example, the anti-blocking agent is stearic acid.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. The material temperature is controlled to be 36 ℃, the probiotic particle preparation is coated, and the weight increase of the coating is controlled to be 30% of the weight of the probiotic particle preparation.
Example 2
This embodiment is an improvement on the basis of embodiment 1, and the content described in embodiment 1 is also what this embodiment has, and is not described here in detail.
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 56%;
28 percent of talcum powder;
7% of a plasticizer;
8.9 percent of antisticking agent;
0.1 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this embodiment, the plasticizer is triethyl citrate.
In this example, the anti-blocking agent is stearic acid.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the material temperature to be 36 ℃ to coat the probiotic particle preparation, wherein the weight increase of the coating is controlled to be 25% of the weight of the probiotic particle preparation.
Example 3
This embodiment is an improvement on the basis of embodiment 1, and the content described in embodiment 1 is also what this embodiment has, and is not described here in detail.
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
69.9% of methacrylic acid and ethyl acrylate copolymer;
20% of talcum powder;
8% of a plasticizer;
2% of an anti-sticking agent;
0.1 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this embodiment, the plasticizer is triethyl citrate.
In this example, the anti-blocking agent is stearic acid.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the material temperature to be 35 ℃ to coat the probiotic particle preparation, wherein the weight increase of the coating is controlled to be 20% of the weight of the probiotic particle preparation.
Example 4
This embodiment is an improvement on the basis of embodiment 1, and the content described in embodiment 1 is also what this embodiment has, and is not described here in detail.
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 65.02%;
22.5 percent of talcum powder;
6.4% of a plasticizer;
6% of an anti-sticking agent;
0.08 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this example, the plasticizer is propylene glycol.
In this embodiment, the anti-sticking agent is silicon dioxide.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the material temperature to be 35.5 ℃ to coat the probiotic particle preparation, wherein the weight increase of the coating is controlled to be 23% of the weight of the probiotic particle preparation.
Example 5
This embodiment is an improvement on the basis of embodiment 1, and the content described in embodiment 1 is also what this embodiment has, and is not described here in detail.
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
55.74% methacrylic acid and ethyl acrylate copolymer;
25% of talcum powder;
7.2% of a plasticizer;
12% of an anti-sticking agent;
0.06 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this example, the plasticizer is triacetin.
In this embodiment, the anti-sticking agent is silica gel micropowder.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the material temperature to be 35 ℃ to coat the probiotic particle preparation, wherein the weight increase of the coating is controlled to be 26% of the weight of the probiotic particle preparation.
Example 6
This embodiment is an improvement on the basis of embodiment 1, and the content described in embodiment 1 is also what this embodiment has, and is not described here in detail.
The film coating premix of the probiotic preparation comprises the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 70%;
20.95 percent of talcum powder;
6% of a plasticizer;
3% of an anti-sticking agent;
0.05 percent of dimethyl silicone oil.
In this embodiment, the ratio of the methacrylic acid-ethyl acrylate copolymer is, in mass percent, 1: 1.
in this embodiment, the plasticizer is polyethylene glycol and triethyl citrate at a mass ratio of 1: 1.
In this embodiment, the anti-sticking agent is stearic acid and silica in a mass ratio of 1: 1.
The preparation method of the film coating premix of the probiotic preparation in this embodiment includes the following steps:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and stearic acid according to mass percent, placing the copolymer, the talcum powder and the stearic acid in a high-efficiency granulator, starting stirring equipment, and stirring and mixing for 10-15 minutes to ensure that the mixture is uniform. Adding triethyl citrate and dimethyl silicone oil into a high-efficiency granulator according to the formula amount, and mixing for 10-15 minutes. And preparing the film coating premix into coating liquid with the solid content of 16% by using purified water, and stirring the coating liquid for 45 minutes by using stirring slurry with the rotating speed of 1000-1500 rpm.
The application of the film coating premix of the probiotic preparation, which is described in this embodiment, is to coat the probiotic preparation.
The probiotic preparation is placed in a fluidized bed for testing, the fluidized bed is started, the frequency of an exhaust fan of the fluidized bed and the atomization of a spray gun are controlled, and the material in a central flow guide pipe of the fluidized bed flows uniformly. And controlling the material temperature to be 35.8 ℃, coating the probiotic particle preparation, wherein the weight increment of the coating is controlled to be 28% of the weight of the probiotic particle preparation.
The stability test is performed on the preferable example 1, the uncoated probiotic particles (25-30 meshes) are used as a comparative example 1, the coating prepared by the microcapsule technology with sodium alginate as a wall material is used as a comparative example 2, the experiment design is that the probiotic survival rate is measured, the probiotic particles are placed in a stabilization box, the temperature is 40 +/-2 ℃, the humidity is 75% +/-5%, the data are examined for 15 days, and the experiment results are as follows:
the stability test is performed on the above preferred example 2, the uncoated probiotic particles (25-30 mesh) are used as a comparative example 3, the coating prepared by the microcapsule technology using sodium alginate as a wall material is used as a comparative example 4, the experimental design is that the probiotic survival rate is measured, the probiotic particles are all placed in a stabilization box at the temperature of 40 +/-2 ℃ and the humidity of 75% +/-5%, the data of 15 days are examined, and the experimental results are as follows:
the stability test is performed on the above preferred example 3, the uncoated probiotic particles (25-30 mesh) are used as a comparative example 5, the coating prepared by the microcapsule technology using sodium alginate as a wall material is used as a comparative example 6, the test is designed to be carried out by placing the probiotic particles in a stabilizing box at the temperature of 40 +/-2 ℃ and the humidity of 75% +/-5% in terms of the survival rate of the probiotic bacteria, and the data of 15 days are examined, and the test results are as follows:
from the data, it can be concluded that the long-term storage of the probiotic preparation in the uncoated state causes the phenomenon of decreased survival rate due to factors such as oxygen, temperature and humidity in the air, and also causes the inactivation due to the erosion of gastric acid when reaching the stomach of the animal. The phenomenon is thoroughly improved after the probiotic preparation film coating premix is coated.
The uncoated probiotic preparation completely loses activity when the experiment is carried out for 10 days, and the probiotic preparation coated by the film coating technology has the strongest capability of preventing the probiotic preparation from being influenced by temperature and humidity; meanwhile, the activity of the probiotic preparation after film coating after artificial gastric juice test on day 15 is far greater than that of the probiotic preparation by microcapsule technology.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.
Claims (5)
1. A film coating premix of a probiotic preparation is characterized by comprising the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 52%;
30% of talcum powder;
6% of triethyl citrate;
11.95 percent of stearic acid;
0.05 percent of dimethyl silicone oil;
the proportion of the methacrylic acid and ethyl acrylate copolymer is 1: 1.
2. a film coating premix of a probiotic preparation is characterized by comprising the following components in percentage by mass:
methacrylic acid and ethyl acrylate copolymer 56%;
28 percent of talcum powder;
7% of triethyl citrate;
8.9 percent of stearic acid;
0.1 percent of dimethyl silicone oil;
the proportion of the methacrylic acid and ethyl acrylate copolymer is 1: 1.
3. a film coating premix of a probiotic preparation is characterized by comprising the following components in percentage by mass:
69.9% of methacrylic acid and ethyl acrylate copolymer;
20% of talcum powder;
8% of triethyl citrate;
2% of stearic acid;
0.1 percent of dimethyl silicone oil;
the proportion of the methacrylic acid and ethyl acrylate copolymer is 1: 1.
4. a method for preparing a film coated premix of a probiotic preparation according to any of claims 1 to 3, comprising the steps of:
weighing methacrylic acid and ethyl acrylate copolymer, talcum powder and anti-sticking agent according to the mass percentage, and uniformly stirring to obtain a mixed solid material;
and weighing a plasticizer according to the mass percentage, adding the dimethyl silicone oil into the mixed solid material, and uniformly stirring to obtain the film coating premix of the probiotic preparation.
5. Use of a film coated premix of a probiotic preparation according to any of claims 1 to 3 for coating a probiotic preparation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710538104.1A CN107307429B (en) | 2017-07-04 | 2017-07-04 | Film coating premix of probiotic preparation and preparation method and application thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710538104.1A CN107307429B (en) | 2017-07-04 | 2017-07-04 | Film coating premix of probiotic preparation and preparation method and application thereof |
Publications (2)
| Publication Number | Publication Date |
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| CN107307429A CN107307429A (en) | 2017-11-03 |
| CN107307429B true CN107307429B (en) | 2020-09-22 |
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| CN109464425B (en) * | 2018-12-29 | 2021-02-05 | 上海交大昂立股份有限公司 | Probiotic embedded particle and preparation method thereof |
| CN112121067A (en) * | 2020-11-16 | 2020-12-25 | 深圳科兴药业有限公司 | Bifidobacterium infantis microcapsule and preparation method thereof |
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| CA2456864A1 (en) * | 2001-06-19 | 2002-12-27 | Advanced Bioadjuvants, Llc | Adjuvant composition for mucosal and injection delivered vaccines |
| WO2004022031A2 (en) * | 2002-09-06 | 2004-03-18 | Canacure Corporation | Stable probiotic microsphere compositions and their methods of preparation |
| CN104042586A (en) * | 2014-06-08 | 2014-09-17 | 浙江华海药业股份有限公司 | Paroxetine enteric-coated and sustained-release tablet and preparation method thereof |
| CN104546946A (en) * | 2014-12-17 | 2015-04-29 | 内蒙古双奇药业股份有限公司 | Preparation method of room-temperature preserved bifidobacteria enteric microcapsule |
| CN105120847A (en) * | 2013-03-14 | 2015-12-02 | 塞拉拜姆有限责任公司 | Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2456864A1 (en) * | 2001-06-19 | 2002-12-27 | Advanced Bioadjuvants, Llc | Adjuvant composition for mucosal and injection delivered vaccines |
| WO2004022031A2 (en) * | 2002-09-06 | 2004-03-18 | Canacure Corporation | Stable probiotic microsphere compositions and their methods of preparation |
| CN105120847A (en) * | 2013-03-14 | 2015-12-02 | 塞拉拜姆有限责任公司 | Targeted gastrointestinal tract delivery of probiotic organisms and/or therapeutic agents |
| CN104042586A (en) * | 2014-06-08 | 2014-09-17 | 浙江华海药业股份有限公司 | Paroxetine enteric-coated and sustained-release tablet and preparation method thereof |
| CN104546946A (en) * | 2014-12-17 | 2015-04-29 | 内蒙古双奇药业股份有限公司 | Preparation method of room-temperature preserved bifidobacteria enteric microcapsule |
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