CN107300787B - The regulation method and apparatus of 3 d light fields between the double-deck biological scattering tissue - Google Patents
The regulation method and apparatus of 3 d light fields between the double-deck biological scattering tissue Download PDFInfo
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Abstract
A kind of device and method for realizing 3 d light fields regulation between the double-deck biological scattering tissue, include: (1) laser beam focus is formed to a point light source on first layer biology scattering tissue, calculates wavefront distribution of the spherical wave of point light source transmitting on second layer biology scattering tissue；(2) the interference pattern of the scattering light and a branch of planar light that are emitted with cameras record from second layer biology scattering tissue；(3) computer extracts the wavefront distributed intelligence of scattering light from interference pattern, obtains the phase-conjugate wave front distribution of scattering light；(4) the wavefront for calculating it in second layer biological tissue surface is distributed；(5) the wavefront that wavefront distribution stacks up to obtain width synthesis is distributed, by the wavefront distributed load to spatial light modulator of synthesis, spatial light modulator modulation step (2) in planar light and reflexed to second layer biological tissue.The present invention can realize light field regulation between bilayer biology scattering tissue, with the characteristics of optical diffraction limit precision, non-intrusion type regulates and controls with three-dimensional.
Description
Technical field
This method belongs to Photobiology field, the regulation method of especially a kind of 3 d light fields between the double-deck biological scattering tissue
And device.
Background technique
Time reversal ultrasonic wave added light focus method can converge to coherent light in biological scatterer, form one and highlight
The optical focus of degree can carry out laser surgey, scanning imagery, photo-thermal treatment and light genetics research using the focus.This method
Frequency displacement afterwards occurs principle by two acousto-optic modulators (AOM1 and AOM2) for sample light S as shown in Figure 1:, scatters after irradiating sample,
The ultrasonic wave that a branch of focusing is squeezed into from sample side, the scattering light frequency in ultrasound focus is by ultrasonic modulation, the modulation light
With the reference light R of a branch of same frequency in nonlinear crystal (such as BSO crystal) internal interference after outgoing sample, interference pattern is written into crystalline substance
Body；Later with a branch of inverting light R with reference light R conjugation*It irradiates nonlinear crystal and reads interference information, form frequency modulation(PFM) and dissipate
The phase-conjugation light for penetrating light converges to ultrasound focus position in scattering sample.
This method can surmount scattering the limit depth on converged light, imaging or light are scanned in deep tissues
Thermotherapy.The disadvantage is that being zero dimension to the regulation of light field, i.e., can only focus light on a point；Optical focus limited size is in ultrasound
The probe limit of resolution.
Transmission matrix measurement method is realized by the input/output relation of calibration scattering layer two sides to some face after scattering layer
On light field regulation.This method principle is as shown in Figure 2: placing a camera (CCD) and a sky respectively in scattering sample two sides
Between optical modulator (SLM), the light field of laser incident sample after being reflected by spatial light modulator, transmission recorded by camera (CCD)；
One group of orthogonal basis is selected to be input on SLM one by one, each inputs corresponding output field under base with camera measurement, by input and output
Relationship constitutes the transmission matrix of scattering sample as matrix element；It can be released from the output field of desired realization is counter according to transmission matrix
Input field on corresponding SLM.
Light can be converged to multiple points after scattering layer on some face simultaneously by this method, realize two-dimension light field regulation,
Resolution ratio is up to optical diffraction limit.The disadvantage is that needing a camera being implanted to scattering sample in collision matrix calibration phase
It afterwards, therefore is intrusive.
[bibliography: 1.Xu, X., Liu, H.&Wang, L.V.Time-reversed ultrasonically
encoded optical focusing into scattering media.Nat.Photonics 5,154–157(2011)
.2.Popoff,S.M.et al.Measuring the transmission matrix in optics:an approach
to the study and control of light propagation in disordered
media.Phys.Rev.Lett.104,100601(2010).】
Summary of the invention
The technical problem to be solved in the present invention is that overcoming the defect of above-mentioned first technology, provide a kind of scattered in the double-deck biology
Penetrate the method and apparatus that 3 d light fields regulate and control between tissue.This method has three-dimensional regulation, optical diffraction limit precision and non-intruding
The characteristics of formula.
Technical solution of the invention is as follows:
A method of 3 d light fields regulation is realized between the double-deck biological scattering tissue, it is characterized in that this method is specifically wrapped
Include the following steps:
(1) laser beam focus is formed into a point light source on first layer biology scattering tissue with a lens, according to two layers
Wavefront distribution of the spherical wave of the distance computation of tissue point light source transmitting on second layer biology scattering tissue；
(2) the interference pattern of the scattering light and a branch of planar light that are emitted with cameras record from second layer biology scattering tissue；
(3) computer extracts the wavefront distributed intelligence of scattering light from interference pattern and does the processing of phase conjugation to it, obtains
To the phase-conjugate wave front distribution of scattering light；
(4) the 3 d light fields are calculated according to the 3 d light fields distribution for wanting to realize and be distributed in second layer biological tissue surface
Wavefront distribution；
(5) the wavefront of step (1) is distributed by computer, (4) the distribution of the phase-conjugate wave front of the scattering light of step (3) and step are counted
The wavefront distribution linear superposition that the 3 d light fields of calculation are distributed in second layer biological tissue surface obtains the wavefront distribution of width synthesis
And by the control software of spatial light modulator by the wavefront distributed load to spatial light modulator of the synthesis, the spatial light tune
Device processed according to load wavefront distribution to step (2) in the wavefront distribution of planar light be modulated, while being reflexed to second
Layer biological tissue layer, spheric wave front and conjugate wavefront ingredient in the light wave can offset the scattering effect of second layer biological tissue layer
Should to make the wavefront of 3 d light fields it is lossless by the organized layer, the wavefront of 3 d light fields is saturating two layers biological tissue's interlayer
The 3 d light fields distribution for wanting to realize is formed after prescribed space Free propagation.
For the device of the above-mentioned method for realizing 3 d light fields regulation between the double-deck biological scattering tissue, which includes swashing
Light device, the output light direction along laser are successively spatial filter, the first reflecting mirror and the adjustable beam splitter of splitting ratio, this point
Incident light is divided into sample arm and reference path by beam device, and the sample arm is successively half-wave plate, the second reflecting mirror, phase
Delayer, collector lens, biological sample, the first imaging len and bundling device, the reference path are successively beam expander, third
Reflecting mirror and bundling device, the output light direction along the bundling device are digital phase conjugate lens, digital phase conjugation
Mirror includes beam splitter, spatial light modulator, the second imaging len and camera, and the laser of the laser transmitting is by the sky
Between filter filter at the light beam of single transverse mode, which is divided into sample arm and reference path by the adjustable beam splitter of splitting ratio, institute
The sample arm and reference path stated merge into all the way through bundling device, co-incident digital phase conjugate lens；The biology
Sample is made of the first biological tissue layer and the second biological tissue layer, and the outer surface of the second biological tissue layer passes through described
Imaging len be imaged on the surface of spatial light modulator, the reflected light through the spatial light modulator surface is through the beam splitting
By the way that on the lens imaging to camera, the reference path is by the light and sky after the bundling device reflection after device reflection
Between light modulator surface it is vertical；The camera, spatial light modulator are connect and are carried out by cable with the computer
Data transmission.
The wavelength of the laser can be visible light or near-infrared.
The biological sample is made of two layers of biological scattering tissue that transparent spacer layer is opened, and the hyaline layer is transparent
Gas blanket, transparency liquid layer or transparent colloid layer, described two layers biological scattering tissue is gastral cavity, enteric cavity, animal ovum or crust
Class insect.
Technical effect of the invention:
The present invention mainly has the advantages of following several respects compared with first technology:
It 1, can three-dimensional regulation light field.The method and dress of 3 d light fields regulation are realized between the double-deck biological scattering tissue of the invention
It sets, memory effect and optical phase conjugation technology of the principle based on scattering medium, utilizes the point light source of biological tissue surface
Phase conjugate wave can will think the lossless 3D region being transferred near point light source of optical field distribution to be prepared as carrier wave
It is interior.And formerly technology can only prepare zero dimension light field (point) or two-dimension light field (face) after scattering medium.
2, non-intrusion type.Realize that the method and apparatus of 3 d light fields regulation do not need between the double-deck biological scattering tissue of the invention
It is inserted into any substance or instrument inside biological scattering tissue, therefore biological tissue will not be damaged.And the first skill in part
Art needs to be implanted into fluorescent molecule, dyestuff or insertion camera in biological tissue.
3, there is optical diffraction limit precision.Formerly technology needs ultrasound to be used as auxiliary, therefore its light field control essence for part
Degree is limited to the resolution ratio of ultrasonic probe.The method and apparatus of 3 d light fields regulation are realized between the double-deck biological scattering tissue of the invention
Without ultrasound as auxiliary, control precision is not limited by the ultrasonic limit of resolution, and is limited only in optical resolution limit.
4, device is simple, low in cost.First non-intrusion type light field regulation method needs ultrasound as auxiliary, ultrasonic
Introducing needs to increase many instruments, such as ultrasonic probe, in addition source of ultrasound signal and signal amplifier etc. also need to consider ultrasonic arteries and veins
Stationary problem between punching, laser pulse, camera and spatial light modulator.It is realized between the double-deck biological scattering tissue of the invention three-dimensional
The method and apparatus of light field regulation do pre-calibration to system using the point light source of biological tissue surface, without introducing ultrasound.
5, time-consuming short.Realize that the method and apparatus of 3 d light fields regulation only need to be to life between the double-deck biological scattering tissue of the invention
One point of object tissue surface, which does pre-calibration, light field tune is realized in the 3D region near the point using memory effect
Control, it is time-consuming short.And formerly technology needs to do all the points in some 2 dimensional region after biological scattering tissue pre-calibration just for part
Two-dimension light field regulation may be implemented, therefore time-consuming.
Detailed description of the invention
Fig. 1 is existing time reversal ultrasonic wave added light focus method index path.
Fig. 2 is existing transmission matrix measurement method schematic diagram.
Fig. 3 is the index path of 3 d light fields regulation device between the double-deck biological scattering tissue of the invention.
In figure: 1: laser；2: spatial filter, 201: the first condenser lenses, 202: single-mode polarization maintaining fiber, 203: collimation
Lens；3: the first reflecting mirrors；4: splitting ratio is adjustable beam splitter, 401: the first half-wave plates, 402: polarization beam apparatus；5: sample light
Road, 501: the second half-wave plates, 502: the second reflecting mirrors, 503: adjustable phase retarder, 504: the second condenser lenses, 505: the first
Imaging len；6: reference path, 601: beam expander, 602: third reflecting mirror；7: the double-deck biology scattering sample, 701: the first biologies
Scattering tissue layer, 702: the second biological scattering tissue layers；8: bundling device；9: digital phase conjugate lens, 901: beam splitter, 902:
Spatial light modulator (SLM), 903: the second imaging lens, 904:CCD camera；10: computer.
Fig. 4 is 3 d light fields regulation method flow diagram between the double-deck biological scattering tissue.
Specific embodiment
Below by embodiment, the present invention will be further described, but should not be limited the scope of the invention with this.
First referring to Fig. 3, Fig. 3 is the index path of 3 d light fields regulation device between the double-deck biological scattering tissue of the invention.By
Figure is as it can be seen that the present invention realizes the regulation device of 3 d light fields, including laser 1 between the double-deck biological scattering tissue, along laser 1
Output light direction be successively that spatial filter 2, the first reflecting mirror 3 and the adjustable beam splitter 4 of splitting ratio, the beam splitter 4 will enter
It penetrates light and is divided into sample arm 5 and reference path 6, the sample arm 5 is successively half-wave plate 501, the second reflecting mirror 502, phase
Position delayer 503, collector lens 504, biological sample 7) the first imaging len 505 and bundling device 8, the reference path 6 according to
Secondary is beam expander 601, third reflecting mirror 602 and bundling device 8, and the output light direction along the bundling device 8 is digital phase
Conjugate lens 9, the digital phase conjugate lens 9 include beam splitter 901, spatial light modulator 902, the second imaging len 903 and phase
Machine 904, the laser that the laser 1 emits is by the filter of spatial filter 2 at the light beam of single transverse mode, and the light beam is by beam splitting
It is divided into sample arm 5 and reference path 6 than adjustable beam splitter 4, the sample arm 5 and reference path 6 are converged through bundling device 8
It synthesizes all the way, co-incident digital phase conjugate lens 9；The biological sample 7 is raw by the first biological tissue layer 701 and second
Object organized layer 702 is constituted, and the outer surface of the second biological tissue layer 702 is imaged by first imaging len 505
Onto the surface of spatial light modulator 902, the reflected light through 902 surface of spatial light modulator is anti-through the beam splitter 901
It is imaged on camera 904 after penetrating by second imaging len 903, the reference path is anti-by the bundling device 8
Light after penetrating is vertical with 902 surface of spatial light modulator；Camera 904, spatial light modulator 902 and the computer
10 are connected and are carried out data transmission by cable.
In the present embodiment
Laser 1 emits the laser of 532nm, coherence length 50m, output power 100mW.Spatial filter 2 by
Non-spherical lens 201, single-mode polarization maintaining fiber 202 and collimation lens 203 form.Splitting ratio is adjustable beam splitter 4 is by 401 He of half-wave plate
Polarization beam apparatus 402 forms, and rotatable halfwave plate 401 can change the polarization state of incident light to change the beam splitting of polarization beam apparatus
Than.
Sample arm 5 is by half-wave plate 501, reflecting mirror 502, adjustable all-wave liquid crystal retarder 503,504 and of non-spherical lens
Commercial micro-lens 505 forms；The polarization state that half-wave plate 501 adjusts sample light keeps it identical as reference polarization state (horizontal inclined
Vibration), so as to be interfered with reference light；Adjustable all-wave liquid crystal retarder 503 controls between sample arm and reference path
Phase difference；Sample light is focused on inner surface (the right table of the first biological scattering tissue layer 701 of sample by non-spherical lens 504
Face)；Second biological 702 outer surface (right surface) of scattering tissue layer is imaged onto the spatial light tune by commercial micro-lens 505
On 902 surface of device processed.Reference path 6 is made of beam expander 601 and third reflecting mirror 602, and laser passes through diameter after beam expander 601
For 34mm.
The digital phase conjugate lens 9 are by polarization-independent beam splitting device 901, pure phase type spatial light modulator 902, quotient
It is constituted with micro-lens 903 and CCD camera 904.Reference path by bundling device 8 reflect after with pure phase type spatial light modulator
902 surfaces are vertical.The reflected light on pure 902 surface of phase type spatial light modulator passes through after being reflected by polarization-independent beam splitting device 901
Commercial micro-lens 903 is imaged onto CCD camera 904, and the pixel of CCD camera 904 and pure phase type spatial light modulator
It is aligned one by one between 902 pixel.
The biological scattering tissue is egg membrane, single layer egg membrane is fitted on transparent glass slide as life
The first biological scattering tissue layer 701 or the second biological scattering tissue layer 702 in object sample 7, two layers of biological scattering tissue interval
20 millimeters.
The computer 10 is association's double-core desktop computer, CPU model Intel Core i3-3240, dominant frequency
For 3.40GHz, 8G is inside saved as.
3 d light fields regulate and control method between the double-deck biological scattering tissue of the present embodiment, comprising the following steps:
(1) laser beam focus is formed a point light source, root on the first biological scattering tissue layer 701 by non-spherical lens 504
The spherical wave of point light source transmitting is calculated in the second biology scattering according to the matlab software on the distance computation machine 10 of two layers of tissue
Wavefront distribution in organized layer 702；
(2) the input voltage for adjusting adjustable all-wave liquid crystal retarder 503 makes phase difference between sample arm and reference path
Respectively 0,π,It is dissipated with the record of CCD camera 904 from what the second biological scattering tissue layer 702 was emitted under 4 kinds of phase differences
It penetrates the interference pattern of light and reference light and inputs the computer 10；
(3) the matlab software on computer 10 extracts the wavefront distributed intelligence of scattering light from interference pattern and to it
The processing of phase conjugation is done, the phase-conjugate wave front distribution of scattering light is obtained, wavefront extraction process is as follows:
The intensity distribution of note scattering light is Is, phase distribution isThe intensity distribution of reference light is Ir, phase distribution isRemember that two-way light phase difference is respectively 0,π,When, the interference pattern that CCD camera 904 photographed is respectively I0, I1, I2, I3,
Then its expression formula are as follows:
Do following calculating:
Obtain phase distribution of the scattering light relative to reference light:
Reference light can be approximately planar light, therefore its phase distributionIt can be taken as 0, so having:
(4), according to the 3 d light fields distribution for wanting to realize, iteration Fourier is passed through using the matlab software on computer 10
Algorithm calculates it in the wavefront distribution on the second biological 702 surface of scattering tissue layer, and specific algorithm process is as follows:
One, iteration Fourier calculation is executed respectively using the light distribution on each fault plane of 3 d light fields as constraint condition
Method；
Two, the constraint item in iterative Fourier transform algorithm, using the two-dimensional space on fault plane as real function domain, on the domain
Part is the light distribution of net amplitude type, should using the two-dimensional space on the second biological 702 surface of scattering tissue layer as Fourier
Constraint condition on domain is the constraint of pure phase bit-type；
Three, after iterative algorithm convergence, the corresponding wavefront distribution of all fault planes linearly is stacked up to obtain a two dimension
Subdivision cloth takes the phase distribution of the subdivision cloth as the corresponding wavefront distribution of 3 d light fields；
Computer 10 by matlab software by step (1), (3) (4) wavefront that step is obtained with step is distributed linear superposition
The wavefront for obtaining a width synthesis be distributed and passes through the control software of spatial light modulator for the wavefront distributed load of the synthesis to empty
Between on optical modulator, the spatial light modulator according to load wavefront distribution to step (2) in the wavefront of planar light be distributed and carry out
Modulation, while it being reverse back to second layer biological tissue layer, the spheric wave front and conjugate wavefront ingredient in the light wave can be offset
The scattering effect of second layer biological tissue layer, so that the wavefront of 3 d light fields is made nondestructively to pass through the organized layer, 3 d light fields
Wavefront forms the 3 d light fields distribution for wanting realization after the Transparent space Free propagation of two layers of biological tissue's interlayer.
3 d light fields regulation method and apparatus can be in the double-deck biology between experiment shows the double-deck biological scattering tissue of the invention
3 d light fields regulation is realized between scattering tissue, have optical diffraction limit precision, can three-dimensional regulation, non-intrusion type, it is at low cost and
Time-consuming short feature.
Claims (4)
1. a kind of method for realizing 3 d light fields regulation between the double-deck biological scattering tissue, it is characterised in that this method specifically includes
The following steps:
(1) laser beam focus is formed into a point light source on first layer biology scattering tissue with a lens, according to two layers of tissue
The distance computation point light source transmitting spherical wave on second layer biology scattering tissue wavefront distribution；
(2) the interference pattern of the scattering light and a branch of planar light that are emitted with cameras record from second layer biology scattering tissue；
(3) computer extracts the wavefront distributed intelligence of scattering light from interference pattern and does the processing of phase conjugation to it, is dissipated
Penetrate the phase-conjugate wave front distribution of light；
(4) the wave that the 3 d light fields are distributed in second layer biological tissue surface is calculated according to the 3 d light fields distribution for wanting to realize
Preceding distribution；
(5) the phase-conjugate wave front of step wavefront distribution (1), the scattering light of step (3) is distributed by computer and (4) step calculates
The wavefront that the wavefront distribution linear superposition that 3 d light fields are distributed in second layer biological tissue surface obtains width synthesis is distributed and leads to
The control software of spatial light modulator is crossed by the wavefront distributed load to spatial light modulator of the synthesis, the spatial light modulator
According to load wavefront distribution to step (2) in the wavefront distribution of planar light be modulated, while it is raw to be reflexed to the second layer
Object organized layer, the wavefront of 3 d light fields is formed after the Transparent space Free propagation of two layers of biological tissue's interlayer wants the three of realization
Tie up optical field distribution.
2. the device of the method for realizing 3 d light fields regulation described in claim 1 between the double-deck biological scattering tissue, special
Sign is that the device includes laser (1), and the output light direction along laser (1) is successively spatial filter (2), the first reflection
Incident light is divided into sample arm (5) and reference path (6) by mirror (3) and the adjustable beam splitter of splitting ratio (4), the beam splitter (4),
The sample arm (5) is successively half-wave plate (501), the second reflecting mirror (502), phase retarders (503), collector lens
(504), biological sample (7), the first imaging len (505) and bundling device (8), the reference path (6) is successively beam expander
(601), third reflecting mirror (602) and bundling device (8), the output light direction along the bundling device (8) are that digital phase is total
Yoke mirror (9), the digital phase conjugate lens (9) include beam splitter (901), spatial light modulator (902), the second imaging len
(903) and camera (904), the laser of the laser (1) transmitting are filtered the light at single transverse mode by the spatial filter (2)
Beam, the light beam are divided into sample arm (5) and reference path (6), the sample arm by the adjustable beam splitter of splitting ratio (4)
(5) it is merged into all the way with reference path (6) through bundling device (8), co-incident digital phase conjugate lens (9)；The biology
Sample (7) is made of the first biological tissue layer (701) and the second biological tissue layer (702), the second biological tissue layer
(702) outer surface is imaged on the surface of spatial light modulator (902) by first imaging len (505), through this
The reflected light on spatial light modulator (902) surface passes through second imaging len after beam splitter (901) reflection
(903) be imaged on camera (904), the reference path reflected by the bundling device (8) after light and space light modulation
Device (902) surface is vertical；Camera (904), spatial light modulator (902) and the computer (10) passes through cable
It connects and carries out data transmission.
3. according to right want 2 described in the regulation devices of 3 d light fields is realized between the biological scattering tissue of bilayer, it is characterised in that swash
The wavelength of light device (1) is visible light or near-infrared.
4. according to right want 2 described in the regulation devices of 3 d light fields is realized between the biological scattering tissue of bilayer, it is characterised in that institute
The biological sample (7) stated is made of two layers of biological scattering tissue that transparent spacer layer is opened, and the hyaline layer is transparent gas
Layer, transparency liquid layer or transparent colloid layer, described two layers biological scattering tissue is gastral cavity, enteric cavity, animal ovum or shell-fish elder brother
Worm.
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