CN107300760A - A kind of polarized light microscopy imaging device and method - Google Patents
A kind of polarized light microscopy imaging device and method Download PDFInfo
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- CN107300760A CN107300760A CN201610237356.6A CN201610237356A CN107300760A CN 107300760 A CN107300760 A CN 107300760A CN 201610237356 A CN201610237356 A CN 201610237356A CN 107300760 A CN107300760 A CN 107300760A
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- lcd
- sample
- msubsup
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- microcobjective
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/0004—Microscopes specially adapted for specific applications
- G02B21/0092—Polarisation microscopes
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/368—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements details of associated display arrangements, e.g. mounting of LCD monitor
Abstract
The invention discloses a kind of polarized light microscopy imaging device and method.The device includes the LCD set gradually from bottom to up, sample stage, microcobjective, tube lens, camera, computer；Wherein LCD is centrally located on the central axis of microcobjective, tube lens；LCD and camera are connected with computer.Method is：Sample to be imaged is placed in sample stage, computer sends control signal to LCD, LCD is set to produce the lighting pattern of needs, collected through sample stage by microcobjective, and carry out being incident to lens after zooming into picture, computer drives camera is sampled to the sample image through lens, and the sample image of collection is shown that observer is watched the sample image of computer display by camera by data wire input computer.Simple in construction, cost of the invention is low, imaging effect is good, can realize that polarized light microscopy is watched to sample.
Description
Technical field
The invention belongs to optical microphotograph technical field of imaging, particularly a kind of polarized light microscopy imaging device and method.
Background introduction
Microscope already penetrates into all trades and professions of ours at one's side as a kind of general observation, metrical instrument, its application
In, it is today's society " materialization judge ", is one " key for exploring microcosmos " again.As the mankind observe the material world
Strided forward towards micron, nanoscale, to small items three-dimensional observation the need for promote the development of stereo micrography imaging technology.Partially
Photoimaging, LED array imaging are two kinds of relatively common micro imaging methods, and its corresponding microscope is referred to as polarized light microscopy
Mirror and LED array microscope.Petrographic microscope is so-called transparent a kind of micro- with opaque anisotropic material for studying
Mirror.Mainly for detection of the material with birefringence, such as filament, spindle, collagen, chromosome (Yin Sici, Ruan's tin
Root, Sun Chengzhi waits fibril angle [J] forest-sciences of application determination of polarized light microscopy lumber fibre cell walls, 1986,22 (2):
209-212.).Traditional petrographic microscope need to add polarizer (polarizer) in input path, make the microscopical illuminating ray be
Analyzer (polarization direction polarizer vertical with the polarizer) is added in linearly polarized light, imaging optical path, unirefringence is observed
Material when, because two polarizers are vertical, light is can't see in microscope, and when being put into birefringence material, due to
Deflected when light is by this kind of material, therefore just can detect this object.
Although traditional petrographic microscope possesses the function not available for traditional light field microscope, this needs micro- light path
In add some optical elements, such as：Annular diaphragm etc., this just virtually adds the complexity of optical path adjusting.Particularly differ
Annular diaphragm size in microscope needs individually to match with each object lens, if there is the object lens of 4 different multiplyings in system,
Need to be equipped with four kinds of various sizes of annular diaphragms to match therewith.Obviously, this causes the structure of condenser to become increasingly multiple
Miscellaneous, component number is more and more, cost also more and more higher therewith.This complicated optical collector structure generally requires skilled micro-
Mirror worker is operated, and needs put into practice, corrected for the difference of sample and the difference of object lens, there is complex operation,
The problem of tedious process.
The content of the invention
It is an object of the invention to provide a kind of polarized light microscopy imaging device that simple in construction, cost is low, imaging effect is good
And method, realize that polarized light microscopy is watched to sample.
The technical solution for realizing the object of the invention is：A kind of polarized light microscopy imaging device, including LCD,
Sample stage, microcobjective, tube lens, camera and computer；Wherein LCD, sample stage, micro- thing
Mirror, tube lens, camera are set gradually from bottom to up, and LCD is centrally located in microcobjective, tube lens
On heart axis；LCD and camera are connected with computer；
Sample to be imaged is placed in sample stage, computer sends control signal to LCD, makes LCD liquid
Crystal panel produces lighting pattern, and the illumination light of the lighting pattern is collected through sample stage by microcobjective, and microcobjective will
The illumination light of collection is incident to tube lens after being amplified, computer drives camera enters to the sample image through tube lens
The sample image of collection is shown that observer is to Computer display by row sampling, camera by data wire input computer
Sample image carries out polarized light microscopy viewing.
Preferably, the size of the LCD is that there is provided monochromatic patterned illumination or multicolor patterns by 70mm × 110mm
Illuminate simultaneously.
Preferably, vertical range of the LCD apart from sample stage upper surface is 30~35mm.
Preferably, the CCD camera lenses of the camera are located on the back focal plane of tube lens.
A kind of micro imaging method based on polarized light microscopy imaging device, step is as follows：
Step 1, a linear polarizer is inserted between microcobjective and sample stage, the linear polarizer is kept with optical axis
Vertically；
Step 2, the position of LCD is marked, makes the pixel area in LCD central, circular region
Domain printing opacity, the area of the border circular areas is 2 times of the condenser aperture diaphragm of microcobjective；
Step 3, any sample is not placed in sample stage, linear polarizer, the sample in microcobjective visual field is rotated
Product are changed into dark completely；
Step 4, sample to be imaged is placed in sample stage, computer sends control signal to LCD, made
LCD produces lighting pattern, and the illumination light of the lighting pattern is collected through sample stage by microcobjective；
Step 5, microcobjective is incident to tube lens, computer drives camera pair after the illumination light of collection is amplified
Sample image through tube lens is sampled, and camera is shown the sample image of collection by data wire input computer
Show, observer carries out polarized light microscopy viewing to the sample image of Computer display and can obtain phase contrast micro-imaging result.
Further, the position of LCD is marked described in step 2, it is specific as follows：
(1) coordinate system xoy is initially set up, origin of coordinates o is located at the center of LCD；
(2) for any one pixel P, its position coordinates is (Px,Py), define the illumination numerical corresponding to pixel P
Aperture NAiFor：
Wherein, f represents the condenser focal length of microcobjective, θiBy the angle of the pixel P light irradiation samples sent；
(3) each pixel P illumination numerical aperture NAiDetermine the angle, θ for the light irradiation sample that P is senti：
Compared with prior art, its remarkable advantage is the present invention：(1) attached extra optical element such as ring light is not needed
Door screen and microlens array, simple in construction, cost are low；(2) imaging effect is good, can realize that polarized light microscopy is watched to sample.
Brief description of the drawings
The structural representation of Fig. 1 polarized light microscopy imaging devices of the present invention.
Fig. 2 is the coordinate system schematic diagram of each pixel in LCD of the invention.
Fig. 3 is the display figure of LCD in polarized light microscopy imaging device of the invention.
Embodiment
Below in conjunction with the accompanying drawings and specific embodiment is described in further detail to the present invention.
With reference to Fig. 1, stereo micrography imaging device of the present invention, including LCD 1, sample stage 2, microcobjective
3rd, tube lens 4, camera 5 and computer 6；Wherein LCD 1, sample stage 2, microcobjective 3, tube lens 4,
Camera 5 is set gradually from bottom to up, and LCD 1 is centrally located at microcobjective 3, the central axis of tube lens 4
On；LCD 1 and camera 5 are connected with computer 6；
Sample to be imaged is placed in sample stage 2, computer 6 sends control signal to LCD 1, makes LCD
Liquid crystal panel 1 produces lighting pattern, and the illumination light of the lighting pattern is collected through sample stage 2 by microcobjective 3, micro- thing
Mirror 3 is incident to tube lens 4 after the illumination light of collection is amplified, 5 pairs of the driving of computer 6 camera passes through tube lens 4
Sample image is sampled, and camera 5 is shown the sample image of collection by data wire input computer 6, observer couple
The sample image that computer 6 is shown carries out polarized light microscopy viewing.
Preferably, the size of the LCD 1 is that there is provided monochromatic patterned illumination or multicolor patterns by 70mm × 110mm
Illuminate simultaneously.
Preferably, vertical range of the LCD 1 apart from the upper surface of sample stage 2 is 30~35mm.
Preferably, the CCD camera lenses of the camera 5 are located on the back focal plane of tube lens 5.
A kind of micro imaging method based on polarized light microscopy imaging device described in claim 1, step is as follows：
Step 1, a linear polarizer is inserted between microcobjective 3 and sample stage 2, the linear polarizer is protected with optical axis
Hold vertical；
Step 2, the position of LCD 1 is marked, makes the pixel in the central, circular region of LCD 1
Region printing opacity, the area of the border circular areas is 2 times of the condenser aperture diaphragm of microcobjective 3；
Step 3, any sample is not placed in sample stage 2, linear polarizer is rotated, until in the visual field of microcobjective 3
Sample is changed into dark completely；
Step 4, sample to be imaged is placed in sample stage 2, computer 6 sends control letter to LCD 1
Number, LCD 1 is produced lighting pattern, the illumination light of the lighting pattern is received through sample stage 2 by microcobjective 3
Collection；
Step 5, microcobjective 3 is incident to tube lens 4, the driving phase of computer 6 after the illumination light of collection is amplified
5 pairs of sample images through tube lens 4 of machine are sampled, and the sample image of collection is inputted and calculated by camera 5 by data wire
Machine 6 is shown that the sample image that observer shows to computer 6 carries out polarized light microscopy viewing and can obtain phase contrast micro-imaging
As a result.
Further, the position of LCD 1 is marked described in step 2, it is specific as follows：
(1) coordinate system xoy is initially set up, origin of coordinates o is located at the center of LCD 1；
(2) for any one pixel P, its position coordinates is (Px,Py), define the illumination numerical corresponding to pixel P
Aperture NAiFor：
Wherein, f represents the condenser focal length of microcobjective 3, θiBy the angle of the pixel P light irradiation samples sent；
(3) each pixel P illumination numerical aperture NAiDetermine the angle, θ for the light irradiation sample that P is senti：
Embodiment 1
The invention device is discussed in detail below in conjunction with the accompanying drawings and the step of watching sample polarized light microscopy is realized.
(1) invention device is discussed in detail with reference to accompanying drawing：
With reference to Fig. 1, stereo micrography imaging device of the present invention, including LCD 1, sample stage 2, microcobjective
3rd, tube lens 4, camera 5 and computer 6；Wherein LCD 1, sample stage 2, microcobjective 3, tube lens 4,
Camera 5 is set gradually from bottom to up, and LCD 1 is centrally located at microcobjective 3, the central axis of tube lens 4
On；LCD 1 and camera 5 are connected with computer 6；
Sample to be imaged is placed in sample stage 2, computer 6 sends control signal to LCD 1, makes LCD
The plate of liquid crystal surface 1 produces required lighting pattern, and the illumination light is collected through sample stage by microcobjective 3, microcobjective 3
The illumination light of collection is amplified and is incident to tube lens 4,5 pairs of the driving of computer 6 camera passes through the sample drawing of tube lens 4
As being sampled, the sample image of collection is shown that observer shows to computer 6 by camera 5 by data wire input computer 6
Sample image carry out polarized light microscopy viewing.Before be specifically imaged, it is necessary to which the position of LCD 1 is marked,
Coordinate system is initially set up, the origin of coordinates is located at the center of LCD 1.For any one pixel P, its position coordinates is
(Px,Py), defining the illumination numerical aperture corresponding to the point is
For the programmable microscope optically focused lens device based on LCD 1, f represents the focal length of condenser.Each pixel
P illumination numerical aperture determines the angle of its optical illumination sample sent
The size of LCD 1 is 70mm × 110mm, it is possible to provide monochromatic patterned illumination can also provide multicolor patterns and illuminate simultaneously.LCD
Distance of the liquid crystal panel 1 apart from the upper surface of sample stage 2 is about 32mm.The CCD camera lenses of camera 6 are located at after tube lens 4
On focal plane.Fig. 2 is the coordinate system schematic diagram of each pixel in LCD of the invention.The polarisation that Fig. 3 is the present invention shows
The given pattern of display needed for LCD in micro- imaging device, the center of circle location of pixels coordinate of border circular areas is (Px,Py),
Radius is R.That wherein red dotted portion is represented is the numerical aperture region NA of microcobjectiveobj。
(2) realizing the specific steps of the object presented to the device viewing includes：
Step one：A linear polarizer is inserted in imaging optical path microcobjective 3, and it is vertical with optical axis holding.
Step 2：For the programmable microscope optically focused lens device based on LCD 1, make its central border circular areas
Pixel region printing opacity can (remainder be light tight).The size of the border circular areas is proportional to the big of condenser aperture diaphragm
It is small, it is however generally that outline is less than the numerical aperture of microcobjective 3.
Step 3：Any sample is not placed in sample stage 2, linear polarizer is rotated, until visual field is changed into dark completely.
Step 4：Under this arrangement, sample to be seen is put into, is shot using camera 5, the display of computer 6 is watched
Obtain phase contrast micro-imaging result.
Simple in construction, cost of the invention is low, do not need attached extra optical element such as annular diaphragm and microlens array, into
As effect is good, it can realize that polarized light microscopy is watched to sample.
Claims (6)
1. a kind of polarized light microscopy imaging device, it is characterised in that including LCD (1), sample stage (2), micro- thing
Mirror (3), tube lens (4), camera (5) and computer (6)；Wherein LCD (1), sample stage (2), micro- thing
Mirror (3), tube lens (4), camera (5) are set gradually from bottom to up, and LCD (1) is centrally located at microcobjective
(3), on the central axis of tube lens (4)；LCD (1) and camera (5) are connected with computer (6)；
Sample to be imaged is placed in sample stage (2), computer (6) sends control signal to LCD (1), made
LCD (1) produces lighting pattern, and the illumination light of the lighting pattern is received through sample stage (2) by microcobjective (3)
Collection, microcobjective (3) is incident to tube lens (4), computer (6) driving camera (5) after the illumination light of collection is amplified
Sample image through tube lens (4) is sampled, the sample image of collection is inputted and calculated by camera (5) by data wire
Machine (6) is shown that the sample image that observer shows to computer (6) carries out polarized light microscopy viewing.
2. polarized light microscopy imaging device according to claim 1, it is characterised in that the size of the LCD (1)
Illuminated simultaneously there is provided monochromatic patterned illumination or multicolor patterns for 70mm × 110mm.
3. polarized light microscopy imaging device according to claim 1, it is characterised in that the LCD (1) is apart from sample
The vertical range of product objective table (2) upper surface is 30~35mm.
4. polarized light microscopy imaging device according to claim 1, it is characterised in that the CCD camera lenses of the camera (5) are located at
On the back focal plane of tube lens (5).
5. a kind of micro imaging method based on polarized light microscopy imaging device described in claim 1, it is characterised in that step is such as
Under：
Step 1, a linear polarizer is inserted between microcobjective (3) and sample stage (2), the linear polarizer is protected with optical axis
Hold vertical；
Step 2, the position of LCD (1) is marked, makes the pixel in LCD (1) central, circular region
Region printing opacity, the area of the border circular areas is 2 times of the condenser aperture diaphragm of microcobjective (3)；
Step 3, any sample is not placed in sample stage (2), linear polarizer is rotated, until in microcobjective (3) visual field
Sample is changed into dark completely；
Step 4, sample to be imaged is placed in sample stage (2), computer (6) sends control letter to LCD (1)
Number, LCD (1) is produced lighting pattern, the illumination light of the lighting pattern passes through sample stage (2) by microcobjective
(3) collect；
Step 5, microcobjective (3) is incident to tube lens (4), computer (6) driving after the illumination light of collection is amplified
Camera (5) is sampled to the sample image through tube lens (4), and the sample image of collection is passed through data wire by camera (5)
Input computer (6) is shown that it is i.e. available that the sample image that observer shows to computer (6) carries out polarized light microscopy viewing
Phase contrast micro-imaging result.
6. the polarized light microscopy imaging method according to claim 5, it is characterised in that to LCD described in step 2
(1) position is marked, specific as follows：
(1) coordinate system xoy is initially set up, origin of coordinates o is located at the center of LCD (1)；
(2) for any one pixel P, its position coordinates is (Px,Py), define the illumination numerical aperture corresponding to pixel P
NAiFor：
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Wherein, f represents the condenser focal length of microcobjective (3), θiBy the angle of the pixel P light irradiation samples sent；
(3) each pixel P illumination numerical aperture NAiDetermine the angle, θ for the light irradiation sample that P is senti：
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2
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| CN201610237356.6A CN107300760A (en) | 2016-04-15 | 2016-04-15 | A kind of polarized light microscopy imaging device and method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112596222A (en) * | 2020-12-03 | 2021-04-02 | 崔亚君 | Microscopic shooting method for single and double refraction plant tissue co-imaging |
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| CN203849498U (en) * | 2014-05-26 | 2014-09-24 | 华南农业大学 | Multifunctional digital liquid crystal fluorescent microscope characterized by synchronous displaying of ocular lens and display screen |
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Application publication date: 20171027 |