CN107300542A - The detection means and method of dissolved organic matter concentration in a kind of aquaculture system - Google Patents
The detection means and method of dissolved organic matter concentration in a kind of aquaculture system Download PDFInfo
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- CN107300542A CN107300542A CN201710400044.7A CN201710400044A CN107300542A CN 107300542 A CN107300542 A CN 107300542A CN 201710400044 A CN201710400044 A CN 201710400044A CN 107300542 A CN107300542 A CN 107300542A
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6491—Measuring fluorescence and transmission; Correcting inner filter effect
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Abstract
The invention provides the detection means and method of dissolved organic matter concentration in a kind of aquaculture system, including：Light source cell, light path adjustment unit, fluorescence detection unit and data processing unit.The two beam intensity identical ultraviolet lights that light source cell is sent are injected in water sample room, water sample room after light path adjustment unit is provided with water body to be measured.The fluorescence intensity of two diverse locations is detected by fluorescence detection unit, and transmit fluorescence intensity and corresponding test position to data processing unit, data processing unit is corrected to fluorescence intensity, and the concentration of dissolved organic matter in water body to be measured is calculated by the fluorescence intensity of correction.Reduce the asynchronous error brought of detection.By the correction to fluorescence intensity, the concentration of dissolved organic matter in water body to be measured can accurately be obtained by being measured without the absorbance to water body to be measured, reduce influence of the fluorescence inner filtering effect to result of calculation, it is ensured that the accuracy of result.
Description
Technical field
The present invention relates to the water body detection field in aquaculture, more particularly, to can in a kind of aquaculture system
The detection means and method of soluble organism concentration.
Background technology
Chromophoric dissolved organic matter (Chromophoric Dissolved Organic Matter, CDOM), also known as Huang
Matter, is present in all water bodys.CDOM is a kind of important component in dissolved organic matter storehouse, by detritus acid, fulvic acid
With a series of material compositions such as aromatic polymer.Meanwhile, CDOM is the important light absorbs material of a class, its concentration and group in water body
Into underwater light field can be significantly changed.CDOM is most strong due to the absorbability to ultraviolet light, thus limits harmful to aquatile
UV-B wave band (Ultraviolet B, UV-B) radiation penetration depths, general UV-B wave-length coverage for 280nm~
320nm.CDOM presence, protects aquatile in water body, and the ecosystem of water body is had a significant effect.In addition, CDOM is not
Only there is very strong absorbability to ultraviolet light, the blue light components in the visible ray closed on ultraviolet band are also can absorb, with swimming
The absorption of chlorophyll a and abiotic suspended particulate substance in plant is overlapping, influences water body primary productivity, also disturbs utilization
Ocean Color Remote Sensing technology calculates the biomass of phytoplankton.
The product of the rotten degraded of water plant is the CDOM main sources in breeding water body (being often referred to the water body in pond),
CODM excessive concentrations can cause in pond phytoplankton growth slow, absorb that light is overlapping, influence the photosynthesis of chlorophyll, so that
The dissolved oxygen in water body is caused to reduce, it is very big to the aerobic harm in breeding water body.When CDOM concentration is too low, to purple
The absorption of outer light is reduced, and growth of the ultraviolet radiation to aquatile is adversely affected.
The research method to CDOM mainly has chemical method and physical method at present.Chemical method typically uses absorption method,
Process is sufficiently complex, and can only obtain CDOM main component, limits research of the people to it.Therefore, to CDOM shape
Gradually physical field is cross over into, the research of composition and evolution mechanism from biochemistry.Because CDOM contents are very low in water body, composition
Complexity, physical method produces fluorescence to UV Absorption according to CDOM, utilizes photoelectricity generally based on the optical property of water body
Detector is detected to the intensity of fluorescence, and then obtains the concentration of CDOM in water body.
Prior art is generally detected using sepectrophotofluorometer to the concentration of CDOM in water body, with highly sensitive
Property, operate also fairly simple.But not consider fluorescence inner filtering effect, can cause it is inaccurate to fluorescence intensity measurement, and then
Influence calculates the accuracy of CDOM concentration in obtained water body.
The content of the invention
To overcome above mentioned problem or solving the above problems at least in part, the invention provides a kind of aquaculture system
The detection means of middle dissolved organic matter concentration, including：At light source cell, light path adjustment unit, fluorescence detection unit and numeral
Manage unit；Wherein, the light source cell is used to produce two beam intensity identical ultraviolet lights；The light path adjustment unit is used to adjust
The direction of propagation of the two beam intensities identical ultraviolet light, makes the ultraviolet light become directional light and vertically injects water sample room, with
The water body to be measured in water sample room is set to produce fluorescence through the ultraviolet excitation；Fluorescence detection unit is used to detect the fluorescence received
Intensity, and the fluorescence intensity detected and corresponding test position are transmitted to the digital processing element；At the numeral
Managing unit is used to correct the fluorescence intensity according to the fluorescence intensity and corresponding test position, and according to the fluorescence after correction
The concentration of dissolved organic matter in water body to be measured described in Strength co-mputation.
It is preferred that, the light source cell includes：Ultraviolet source and spectrophotometric unit；The spectrophotometric unit is vertically arranged in described
In detection means, the ultraviolet light that the ultraviolet source is produced irradiates the spectrophotometric unit with the first predetermined angle, passes through described point
The ultraviolet light is divided into two beam intensity identical ultraviolet lights by light unit.
It is preferred that, the light path adjustment unit includes：First level crossing, the second level crossing, the first collimation lens and second
Collimation lens；Wherein, first level crossing is oppositely arranged in the spectrophotometric unit both sides respectively with second level crossing；Institute
The first side for stating water sample room is vertical with the spectrophotometric unit, first collimation lens and second collimation lens with it is described
First side be arranged in parallel.
It is preferred that, the fluorescence detection unit includes：First condenser lens, the second condenser lens, the first photodetector
With the second photodetector；First condenser lens and second condenser lens are parallel with the second side of the water sample room
Set；First photodetector is used to receive assembles obtained fluorescence through first condenser lens；Second photoelectricity
Detector is used to receive assembles obtained fluorescence through second condenser lens；First photodetector and second light
Electric explorer is electrically connected with the data processing unit respectively.
It is preferred that, the detection means also includes：Water sample room water inlet and enter unit for treating water；Water sample room water inlet
It is arranged on a side of the water sample room；It is described enter unit for treating water include：Current limiting plate, filter screen and miillpore filter；It is described
Current limiting plate is used to limit the water body to be measured into the flow velocity during water sample room；The filter screen is used to filter the water body to be measured
In impurity particle；The aperture of the miillpore filter is 0.22um or 0.45um, for filtering the impurity in the water body to be measured
Particulate；The filter screen, current limiting plate and miillpore filter are successively set on the outside of water sample room water inlet, and pass through inlet channel
It is fixed.
It is preferred that, the digital processing element specifically for：According to the fluorescence intensity, corresponding test position and as follows
Fluorescence intensity described in formula correction：
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2Respectively obtained in two different test position detections
Fluorescence intensity, I1And I2Respectively and F1And F2Corresponding test position.
It is preferred that, the digital processing element is additionally operable to：According to the fluorescence intensity after correction and concentration strengths formula, calculate
The concentration of dissolved organic matter in the water body to be measured；
Wherein, the concentration strengths formula is：
F0=5.689C+15.9
Wherein, C is the concentration of dissolved organic matter in the water body to be measured, and unit is mg/L, F0For what is obtained after correction
Fluorescence intensity.
On the other hand, the invention provides a kind of detection method of dissolved organic matter concentration in aquaculture system, bag
Include：Two beam intensity identical ultraviolet lights are respectively become into directional light, and the directional light vertical water specimen chamber one side is entered institute
State water sample room；Water body to be measured is provided with the water sample room；Detected in the another side of the water sample room in the water sample room
The fluorescence intensity that two diverse locations are produced；According to the fluorescence intensity and corresponding test position correction of fluorescence intensity, and root
The concentration of dissolved organic matter in the water body to be measured is calculated according to concentration strengths formula.
It is preferred that, it is described to be specifically included according to the fluorescence intensity and corresponding test position correction of fluorescence intensity：According to
The fluorescence intensity and corresponding test position, the fluorescence intensity after being corrected using equation below：
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2Respectively obtained in two different test position detections
Fluorescence intensity, I1And I2Respectively and F1And F2Corresponding test position.
The detection means and method of dissolved organic matter concentration, light source list in a kind of aquaculture system that the present invention is provided
The two beam intensity identical ultraviolet lights that member is sent inject water sample room after light path adjustment unit, and detect two by fluorescence detection unit
The fluorescence intensity of individual diverse location, and fluorescence intensity and corresponding test position are transmitted to data processing unit, data processing
Unit is corrected to fluorescence intensity, and the concentration of dissolved organic matter in water body to be measured is calculated by the fluorescence intensity of correction.Subtract
The asynchronous error brought of detection is lacked.By the correction to fluorescence intensity, measured without the absorbance to water body to be measured
The concentration of dissolved organic matter in water body to be measured can be accurately obtained, influence of the fluorescence inner filtering effect to result of calculation is reduced,
It ensure that the accuracy of result.The detection means and method that the present invention is provided can be additionally used in the Site Detections such as river, ocean.
Brief description of the drawings
Fig. 1 is the structure of the detecting device figure of CDOM concentration in a kind of aquaculture system that the embodiment of the present invention 1 is provided；
Fig. 2 is the structure of the detecting device figure of CDOM concentration in a kind of aquaculture system that the embodiment of the present invention 2 is provided；
Fig. 3 is the detection method flow chart of CDOM concentration in a kind of aquaculture system that the embodiment of the present invention 4 is provided；
Fig. 4 be the embodiment of the present invention 4 in CDOM concentration and correction after fluorescence intensity fitted figure；
Wherein, 1：Ultraviolet source, 2：Spectrophotometric unit, 3：First level crossing, 4：Second level crossing, 5：First collimation lens,
6：Second collimation lens, 7：Filter screen, 8：Current limiting plate, 9：Miillpore filter, 10：Water sample room water inlet, 11：Water sample room, 12：First gathers
Focus lens, 13：Second condenser lens, 14：First photodetector, 15：Second photodetector, 16：Data processing unit.
Embodiment
With reference to the accompanying drawings and examples, the embodiment to the present invention is described in further detail.Implement below
Example is used to illustrate the present invention, but is not limited to the scope of the present invention.
At present, using sepectrophotofluorometer to aquaculture system (aquaculture system is referred to as into water body below)
Middle chromophoric dissolved organic matter (Chromophoric Dissolved Organic Matter, English abbreviation is CDOM) it is dense
Degree is detected, with high sensitivity, is operated also fairly simple.But the fluorescence inner filtering effect in water body, meeting are not considered
Cause inaccurate to fluorescence intensity measurement, and then influence to calculate the accuracy of CDOM concentration in obtained water body.Wherein, in fluorescence
Filter effect refer to when dissolved organic matter concentration is larger in water body or water body in dissolved organic matter coexisted with other extinction materials when,
Due to dissolved organic matter in water body or other extinction materials for generation fluorescence or injection ultraviolet light absorption and cause
The phenomenon of fluorescent weakening.Following examples are to detect in water body exemplified by CDOM concentration, but the detection means that the present invention is provided
It is not limited to detect the concentration of CDOM in water body with detection method, other that also can detect in water body through ultraviolet light and can swash
Hair produces the concentration of the fluorescent material of fluorescence.
Embodiments of the invention 1 there is provided a kind of detection means of CDOM concentration in water body, including：Light source cell, light path
Adjustment unit, fluorescence detection unit and digital processing element.
Wherein, the light source cell is used to produce two beam intensity identical ultraviolet lights；The light path adjustment unit is used to adjust
The direction of propagation of the whole two beam intensities identical ultraviolet light, makes the ultraviolet light become directional light and vertically injects water sample room,
The CDOM being provided with water sample room in water body to be measured, water body to be measured can produce fluorescence through ultraviolet excitation.Here, water body to be measured is
The measurement sample obtained from water body.
Fluorescence detection unit is used for the intensity for receiving the fluorescence that the fluorescence produced and detection are received, and glimmering by what is detected
Luminous intensity and corresponding test position are transmitted to the digital processing element；The digital processing element is used for according to the fluorescence
Intensity and corresponding test position correct the fluorescence intensity, and are calculated according to the fluorescence intensity after correction in the water body to be measured
CDOM concentration.Here, fluorescence detection unit is electrically connected with digital processing element.
The two beam intensity identical ultraviolet lights that light source cell is sent inject water sample room after light path adjustment unit, and by fluorescence
Detection unit detects the fluorescence intensity of two diverse locations, and fluorescence intensity and corresponding test position are transmitted to data processing
Unit, data processing unit is corrected to fluorescence intensity, and calculating solubility in water body to be measured by the fluorescence intensity of correction has
The concentration of machine thing.Reduce the asynchronous error brought of detection.By the correction to fluorescence intensity, without the suction to water body to be measured
Luminosity, which is measured, can accurately obtain the concentration of dissolved organic matter in water body to be measured, reduce fluorescence inner filtering effect to calculating
As a result influence, it is ensured that the accuracy of result.The detection means that the present invention is provided can be additionally used in the inspection of the scene such as river, ocean
Survey.
Specifically, as shown in figure 1, light source cell includes：Ultraviolet source 1 and spectrophotometric unit 2, light path adjustment unit include：
First level crossing 3, the second level crossing 4, the first collimation lens 5 and the second collimation lens 6.
Wherein, spectrophotometric unit 2 is vertically arranged in detection means, and ultraviolet source 1 irradiates light splitting list with the first predetermined angle
Member 2, the first level crossing 3 and the second level crossing 4 are located at the both sides of spectrophotometric unit 2 respectively, and default into second with spectrophotometric unit 2
Angle, the half of the second predetermined angle and the first predetermined angle adds up to 45 degree, and the first level crossing 3 is relative with the second level crossing 4
Set.It is preferred that, choose the ultraviolet source that wavelength is 320nm.
Here, the water sample room 11 of square structure is chosen, equipped with the water body to be measured obtained from water body in water sample room 11.This
In figure, preferably, collimation lens 5 and collimation lens 6 and the first side of water sample room 11 is set to be arranged in parallel.Collimation lens
5 and collimation lens 6 be used for two beam ultraviolet lights are converged to directional light respectively.
Fluorescence detection unit includes：First condenser lens 12, the second condenser lens 13, the first photodetector 14 and second
Photodetector 15.First condenser lens 12 and the second condenser lens 13 and the second side of water sample room 11 be arranged in parallel.Here
First side refer to that ultraviolet light injects the side that water sample room 11 is passed through.Here second side refers to the fluorescence in water sample room 11
Project in the side that water sample room 11 is passed through, figure and refer to the side adjacent with first side.
First condenser lens 12 and the second condenser lens 13 are used to the fluorescence produced in water sample room 11 converging to the respectively
On one photodetector 14 and the second photodetector 15.First photodetector 14 and the second photodetector 15 are arranged at
First condenser lens 12 and the second side of the condenser lens 13 away from the second side of water sample room 11.
It is preferred that, water sample room is quartz colorimetric utensil, and the transmissivity to light is 190nm~2500nm.Spectrophotometric unit 2 is ultraviolet
The ratio of beam splitter, reflectivity and transmitance is 1:1.
Specifically, such as the first predetermined angle is equal to 45 degree, two beam intensity identicals are divided into after spectrophotometric unit 2 ultraviolet
Light, a branch of ultraviolet light by the first level crossing 3 reflect after through the first collimation lens 5 and first side water sample room injected with directional light
11, another beam ultraviolet light by the second level crossing 4 reflect after through the second collimation lens 6 and second side water sample room injected with directional light
11.To ensure that the ultraviolet luminous energy of two beams injects water sample room 11 with directional light, by the first level crossing 3 and the second level crossing 4 and light splitting list
The angle of member 2 is disposed as 22.5 degree.
Two beam ultraviolet lights are injected behind water sample room 11, due to including CDOM in the water body to be measured in water sample room 11, through ultraviolet light
Irradiation, which is excited, can produce fluorescence.The fluorescence of generation is by the first condenser lens 12 being be arranged in parallel with the second side of water sample room 11 and
Two condenser lenses 13 are converged on the first photodetector 14 and the second photodetector 15 respectively.By the first photodetector 14
It is the fluorescence intensity at 410nm to detect two diverse location wavelength respectively with the second photodetector 15.Here first focus on thoroughly
Mirror 12, the second condenser lens 13 are not especially limited relative to the position of second side.
First photodetector 14 and the second photodetector 15 are electrically connected with data processing unit 16 respectively, will be detected
To fluorescence intensity and corresponding test position transmit to data processing unit 16.Data processing unit 16 is usually at computer
Manage terminal.Here electrical connection can use the connection with RS485 interfaces to be attached.Data processing unit 16 is received respectively
The intensity and corresponding test position for the fluorescence that first photodetector 14 and the detection of the second photodetector 15 are obtained, according to glimmering
Luminous intensity and corresponding test position, are corrected using equation below, the fluorescence intensity after being corrected：
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2Respectively the first photodetector 14 and the second photoelectricity are visited
Survey device 15 and detect obtained fluorescence intensity, I1And I2Respectively described two photodetector fluorescence intensities are F1And F2It is right
The test position answered.
Data processing unit 16 is according to the fluorescence intensity F after correction0And calculate water to be measured using following concentration strengths formula
CDOM concentration in body：
F0=5.689C+15.9
Wherein, the concentration that C is CDOM in water body to be measured, unit is mg/L.
Ultraviolet light is divided into two beam intensity identical ultraviolet lights by the present embodiment by spectrophotometric unit, by irradiating in water sample room
Water body to be measured, excite CDOM in water body to be measured to produce fluorescence, fluorescence intensity detected by two photodetectors,
And fluorescence intensity is corrected, the concentration of CDOM in water body to be measured is calculated according to concentration strengths formula.Ultraviolet source is through undue
Light unit is divided into two beam intensity identical light, the water body to be measured that can be irradiated simultaneously in water sample room, and passes through two photodetections
Device detects the fluorescence for obtaining two diverse locations, reduces the error that asynchronous detection band is come.By the correction to fluorescence intensity,
The concentration of CDOM in water body to be measured can accurately be obtained by being measured without the absorbance to water body to be measured, reduce filter in fluorescence
Influence of the effect to result of calculation, it is ensured that the accuracy of result.The detection means that the present embodiment is provided can be additionally used in river, sea
The Site Detections such as ocean.
As shown in Fig. 2 embodiments of the invention 2 provide a kind of detection means of CDOM concentration in water body, with above-mentioned reality
Differing only in for example is applied, detection means also includes：Water sample room water inlet 10 and enter unit for treating water.Wherein, water sample room intakes
Mouth 10 is arranged on a side of water sample room 11；Entering unit for treating water includes：Filter screen 7, current limiting plate 8 and miillpore filter 9；Filter screen
7 are used to filter the impurity particle in water body to be measured；Current limiting plate 8 is used to limit water sample into flow velocity during water sample room 11；Micropore is filtered
The aperture of film 9 is 0.22um or 0.45um, for filtering the contaminant particles in water body to be measured；Filter screen 7, current limiting plate 8 and micropore filter
Film 9 is successively set on the outside of water sample room water inlet 10, and is fixed by inlet channel.
Water body to be measured flows into water sample room 11 through entering unit for treating water and water sample room water inlet 10.For example, being filtered using filter screen 7
Fall the impurity particle of a diameter of 1mm in water body to be measured, such as algae.The water inlet flow control of water body to be measured is existed by current limiting plate 8
1-2cm/s, the contaminant particles filtered by miillpore filter 9 in water body to be measured enter in water sample room 11.In the present embodiment, by setting
Putting into unit for treating water and water sample room water inlet makes detection means continuously to be detected.
Embodiments of the invention 3 provide a kind of detection means of CDOM concentration in water body, the difference with above-described embodiment
It is only that, detection means also includes：Camera bellows；Detection means is placed in camera bellows in addition to inlet channel, and the surface of camera bellows is provided with
Switch, power interface, external interface, display unit and the opening for fixing the inlet channel；Display unit and data
Processing unit 16 is electrically connected, the concentration obtained for display data processing unit；Power interface is used to connect with external power source, is
Detection means is powered；Switch for controlling the ultraviolet source in detection means luminous or not lighting；The model of external interface
RS485, for connecting external equipment.Detection means directly can will detect obtained CDOM concentration datas after being connected with external equipment
Transmit to external equipment, so that external equipment is used.
In the present embodiment, camera bellows can provide unglazed environment for detection means, make after opening is opened the light in detection means
The fluorescence that only ultraviolet light and CDOM are produced through ultraviolet excitation.Testing result is not disturbed by ambient light, improve detection knot
The accuracy of fruit.
As shown in figure 3, embodiments of the invention 4 provide a kind of detection method of CDOM concentration in water body, including：S31,
Two beam intensity identical ultraviolet lights are respectively become into directional light, and the directional light vertical water specimen chamber one side is entered the water
Specimen chamber；Water body to be measured is provided with the water sample room；S32, is detected in the water sample room in the another side of the water sample room
The fluorescence intensity that two diverse locations are produced；S33, according to the fluorescence intensity and corresponding test position correction of fluorescence intensity,
And the concentration of CDOM in the water body to be measured is calculated according to concentration strengths formula.
Specifically, two beam identical ultraviolet lights refer to intensity identical ultraviolet light, wavelength is 320nm, respectively through two standards
Straight lens become directional light and vertically injected in water sample room, and water sample room is square quartz colorimetric utensil, and transmissivity is 190nm-2500nm.
Water body to be measured in water sample room refers to the measurement sample obtained from water body.
After being excited through ultraviolet light, the CDOM in water body to be measured produces fluorescence, is assembled respectively by two condenser lenses
To two photodetectors, the fluorescence intensity of two diverse locations is detected respectively by two photodetectors, according to detecting
It is the fluorescence intensity and corresponding test position at 410nm to wavelength, is corrected using equation below, it is glimmering after being corrected
Luminous intensity：
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2The fluorescence that respectively two photodetector detections are obtained
Intensity, I1And I2Respectively described two photodetector fluorescence intensities are F1And F2Corresponding test position.
According to the fluorescence intensity F after correction0And the concentration of CDOM in water body to be measured is calculated using following concentration strengths formula：
F0=5.689C+15.9
Wherein, the concentration that C is CDOM in water body to be measured, unit is mg/L.
It is sharp by two beam intensity identical ultraviolet lights vertically into the water sample room equipped with water body to be measured in the present embodiment
The fluorescence intensity and corresponding test position produced in water sample room is detected with two photodetectors；The fluorescence obtained to detection is strong
Degree is corrected, and passes through the concentration of CDOM in concentration strengths formula calculating water body to be measured.It is ultraviolet using two beam intensity identicals
Light, the water body to be measured that can be irradiated simultaneously in water sample room, and two diverse locations are obtained by the detection of two photodetectors
Fluorescence, reduces the error that asynchronous detection band is come.By the correction to fluorescence intensity, enter without the absorbance to water body to be measured
Row measurement can accurately obtain the concentration of CDOM in water body to be measured, reduce influence of the fluorescence inner filtering effect to result of calculation, protect
The accuracy of result is demonstrate,proved.
In above-described embodiment, concentration strengths formula is obtained by the following method：Treating for CDOM concentration is preset by least two groups
Water body is surveyed by entering unit for treating water and the input detection device of water sample room water inlet 10, is respectively obtained by digital processing element 16
Fluorescence intensity after corresponding correction；By fitting obtain the fluorescence intensity after the CDOM concentration and the corresponding correction it
Between relation, obtain concentration strengths formula.
The water body to be measured that 18 groups of difference CDOM concentration are chosen in the present embodiment is tested, after obtained corresponding correction
Fluorescence intensity data is as shown in table 1.
The data obtained using table 1, are fitted to the fluorescence intensity after concentration and correction, from linear fit function,
The standard deviation of fitting is R2=0.9973.As shown in figure 4, wherein, ordinate is the fluorescence intensity after correction, and abscissa is CDOM
Concentration.Obtaining concentration strengths formula is：
F0=5.689C+15.9
Wherein, the concentration that C is CDOM in water body to be measured, unit is mg/L；F0For the fluorescence intensity obtained after correction.
The concentration of table 1 and the fluorescence intensity corresponding table after correction
| Concentration C (mg/L) | Fluorescence intensity F after correction0 |
| 1 | 18 |
| 3 | 30 |
| 5 | 43 |
| 10 | 79 |
| 15 | 106 |
| 20 | 136 |
| 25 | 149 |
| 30 | 189 |
| 35 | 218 |
| 40 | 245 |
| 45 | 282 |
| 50 | 285 |
| 55 | 329 |
| 60 | 362 |
| 65 | 369 |
| 70 | 414 |
| 75 | 442 |
| 80 | 483 |
Finally, method of the invention is only preferably embodiment, is not intended to limit the scope of the present invention.It is all
Within the spirit and principles in the present invention, any modification, equivalent substitution and improvements made etc. should be included in the protection of the present invention
Within the scope of.
Claims (10)
1. the detection means of dissolved organic matter concentration in a kind of aquaculture system, it is characterised in that including：Light source cell,
Light path adjustment unit, fluorescence detection unit and digital processing element；
Wherein, the light source cell is used to produce two beam intensity identical ultraviolet lights；The light path adjustment unit is used to adjust institute
The direction of propagation of two beam intensity identical ultraviolet lights is stated, the ultraviolet light is become directional light and vertically injects water sample room, so that
Water body to be measured in water sample room produces fluorescence through the ultraviolet excitation；
Fluorescence detection unit is used to detecting the intensity of fluorescence received, and by the fluorescence intensity detected and corresponding detecting position
Put and transmit to the digital processing element；The digital processing element is used for according to the fluorescence intensity and corresponding test position
The fluorescence intensity is corrected, and according to the concentration of dissolved organic matter in the fluorescence intensity calculating water body to be measured after correction.
2. detection means according to claim 1, it is characterised in that the light source cell includes：Ultraviolet source and light splitting
Unit；
The spectrophotometric unit is vertically arranged in the detection means, and the ultraviolet light that the ultraviolet source is produced is with the first preset angle
The degree irradiation spectrophotometric unit, is divided into two beam intensity identical ultraviolet lights by the spectrophotometric unit by the ultraviolet light.
3. detection means according to claim 2, it is characterised in that the light path adjustment unit includes：First level crossing,
Second level crossing, the first collimation lens and the second collimation lens；
Wherein, first level crossing is oppositely arranged in the spectrophotometric unit both sides respectively with second level crossing；The water
The first side of specimen chamber is vertical with the spectrophotometric unit, first collimation lens and second collimation lens and described first
Side be arranged in parallel.
4. detection means according to claim 3, it is characterised in that the fluorescence detection unit includes：First focuses on thoroughly
Mirror, the second condenser lens, the first photodetector and the second photodetector；
First condenser lens and second condenser lens and the second side of the water sample room be arranged in parallel；Described first
Photodetector is used to receive assembles obtained fluorescence through first condenser lens；Second photodetector is used to receive
Obtained fluorescence is assembled through second condenser lens；First photodetector and second photodetector respectively with
The data processing unit electrical connection.
5. detection means according to claim 4, it is characterised in that the digital processing element specifically for：
The fluorescence intensity is corrected according to the fluorescence intensity, corresponding test position and equation below：
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<mfrac>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
<mrow>
<msub>
<mi>I</mi>
<mn>2</mn>
</msub>
<mo>-</mo>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
</mrow>
</mfrac>
<mo>|</mo>
</mrow>
</msup>
</mrow>
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2The fluorescence respectively obtained in two different test position detections
Intensity, I1And I2Respectively and F1And F2Corresponding test position.
6. detection means according to claim 5, it is characterised in that the digital processing element is additionally operable to：
According to the fluorescence intensity after correction and concentration strengths formula, the concentration of dissolved organic matter in the water body to be measured is calculated；
Wherein, the concentration strengths formula is：
F0=5.689C+15.9
Wherein, C is the concentration of dissolved organic matter in the water body to be measured, and unit is mg/L.
7. the detection means according to any one of claim 1-6, it is characterised in that also include：Water sample room water inlet and
Enter unit for treating water；
Water sample room water inlet is arranged on a side of the water sample room；
It is described enter unit for treating water include：Current limiting plate, filter screen and miillpore filter；
The current limiting plate is used to limit the water body to be measured into the flow velocity during water sample room；The filter screen is used to filter described
Impurity particle in water body to be measured；The aperture of the miillpore filter is 0.22um or 0.45um, for filtering the water body to be measured
In contaminant particles；
The filter screen, current limiting plate and miillpore filter are successively set on the outside of water sample room water inlet, and pass through inlet channel
It is fixed.
8. the detection method of dissolved organic matter concentration in a kind of aquaculture system, it is characterised in that including：
Two beam intensity identical ultraviolet lights are respectively become into directional light, and the directional light vertical water specimen chamber one side is entered institute
State water sample room；Water body to be measured is provided with the water sample room；
The fluorescence intensity that two diverse locations are produced in the water sample room is detected in the another side of the water sample room；
Treated according to the fluorescence intensity and corresponding test position correction of fluorescence intensity, and according to being calculated concentration strengths formula
Survey the concentration of dissolved organic matter in water body.
9. detection method according to claim 8, it is characterised in that the concentration strengths formula is obtained by the following method
Take：
By the detection means described in the water body to be measured input claim 8 of at least two groups default dissolved organic matter concentration, pass through
Digital processing element respectively obtains the fluorescence intensity after corresponding correction；
Relation between fluorescence intensity after the dissolved organic matter concentration and the corresponding correction is obtained by fitting, obtained
To concentration strengths formula.
10. detection method according to claim 8 or claim 9, it is characterised in that described according to the fluorescence intensity and corresponding
Test position correction of fluorescence intensity is specifically included：
According to the fluorescence intensity and corresponding test position, the fluorescence intensity after being corrected using equation below：
<mrow>
<msub>
<mi>F</mi>
<mn>0</mn>
</msub>
<mo>=</mo>
<msub>
<mi>F</mi>
<mn>1</mn>
</msub>
<mo>&times;</mo>
<msup>
<mrow>
<mo>(</mo>
<msub>
<mi>F</mi>
<mn>1</mn>
</msub>
<mo>/</mo>
<msub>
<mi>F</mi>
<mn>2</mn>
</msub>
<mo>)</mo>
</mrow>
<mrow>
<mo>|</mo>
<mfrac>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
<mrow>
<msub>
<mi>I</mi>
<mn>2</mn>
</msub>
<mo>-</mo>
<msub>
<mi>I</mi>
<mn>1</mn>
</msub>
</mrow>
</mfrac>
<mo>|</mo>
</mrow>
</msup>
</mrow>
Wherein, F0For the fluorescence intensity obtained after correction, F1And F2The fluorescence respectively obtained in two different test position detections
Intensity, I1And I2Respectively and F1And F2Corresponding test position.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064095A (en) * | 2017-06-02 | 2017-08-18 | 安徽大学 | Dual-wavelength quantum dot fluorescent probe furanone detection device and detection method thereof |
| WO2022148252A1 (en) * | 2021-01-11 | 2022-07-14 | 江西师范大学 | Method and apparatus for measuring concentration of dissolved organic carbon in water |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201984034U (en) * | 2010-12-21 | 2011-09-21 | 无锡荣兴科技有限公司 | Water quality detection sensor |
| US8269192B2 (en) * | 2010-02-02 | 2012-09-18 | Mote Marine Laboratory, Inc. | Method and apparatus for determining the presence of optical brighteners in water samples |
| CN102928390A (en) * | 2012-09-27 | 2013-02-13 | 中国农业大学 | On-line detection method and device for chlorophyll concentration in water body based on two detectors |
| CN103278482A (en) * | 2013-04-28 | 2013-09-04 | 燕山大学 | Dual-probe type optical detection device for detecting chlorophyll-a in water |
| CN105300947A (en) * | 2015-11-14 | 2016-02-03 | 常州大学 | Fluorescence spectrum technology-based dissolved organic matter (DOM) water quality monitoring and early warning method |
-
2017
- 2017-05-31 CN CN201710400044.7A patent/CN107300542A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8269192B2 (en) * | 2010-02-02 | 2012-09-18 | Mote Marine Laboratory, Inc. | Method and apparatus for determining the presence of optical brighteners in water samples |
| CN201984034U (en) * | 2010-12-21 | 2011-09-21 | 无锡荣兴科技有限公司 | Water quality detection sensor |
| CN102928390A (en) * | 2012-09-27 | 2013-02-13 | 中国农业大学 | On-line detection method and device for chlorophyll concentration in water body based on two detectors |
| CN103278482A (en) * | 2013-04-28 | 2013-09-04 | 燕山大学 | Dual-probe type optical detection device for detecting chlorophyll-a in water |
| CN105300947A (en) * | 2015-11-14 | 2016-02-03 | 常州大学 | Fluorescence spectrum technology-based dissolved organic matter (DOM) water quality monitoring and early warning method |
Non-Patent Citations (2)
| Title |
|---|
| M.M.PUCHALSKI 等: "Assessment of inner filter effect corrections in fluorimetry", 《FRESENIUS" JOURNAL OF ANALYTICAL CHEMSITRY》 * |
| 柳先平 等: "不同水体中有色可溶性有机物质荧光光谱定量分析研究", 《光谱学与光谱分析》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107064095A (en) * | 2017-06-02 | 2017-08-18 | 安徽大学 | Dual-wavelength quantum dot fluorescent probe furanone detection device and detection method thereof |
| WO2022148252A1 (en) * | 2021-01-11 | 2022-07-14 | 江西师范大学 | Method and apparatus for measuring concentration of dissolved organic carbon in water |
| GB2614769A (en) * | 2021-01-11 | 2023-07-19 | Univ Jiangxi Normal | Method and apparatus for measuring concentration of dissolved organic carbon in water |
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