CN107290286A - A kind of high-velocity scanning confocal imaging system available for spectrum analysis - Google Patents
A kind of high-velocity scanning confocal imaging system available for spectrum analysis Download PDFInfo
- Publication number
- CN107290286A CN107290286A CN201610221778.4A CN201610221778A CN107290286A CN 107290286 A CN107290286 A CN 107290286A CN 201610221778 A CN201610221778 A CN 201610221778A CN 107290286 A CN107290286 A CN 107290286A
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- slit
- copolymerization
- burnt
- dispersion
- light
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- 238000010226 confocal imaging Methods 0.000 title claims abstract description 19
- 238000010183 spectrum analysis Methods 0.000 title claims abstract description 15
- 239000006185 dispersion Substances 0.000 claims abstract description 32
- 238000003384 imaging method Methods 0.000 claims abstract description 29
- 238000007334 copolymerization reaction Methods 0.000 claims abstract description 25
- 230000003595 spectral effect Effects 0.000 claims abstract description 11
- 230000003287 optical effect Effects 0.000 claims description 8
- 238000001914 filtration Methods 0.000 claims description 6
- 238000011835 investigation Methods 0.000 abstract description 2
- 239000000463 material Substances 0.000 abstract description 2
- 238000010586 diagram Methods 0.000 description 11
- 238000001228 spectrum Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 239000004973 liquid crystal related substance Substances 0.000 description 1
- 230000003534 oscillatory effect Effects 0.000 description 1
- 230000008521 reorganization Effects 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01J—MEASUREMENT OF INTENSITY, VELOCITY, SPECTRAL CONTENT, POLARISATION, PHASE OR PULSE CHARACTERISTICS OF INFRARED, VISIBLE OR ULTRAVIOLET LIGHT; COLORIMETRY; RADIATION PYROMETRY
- G01J3/00—Spectrometry; Spectrophotometry; Monochromators; Measuring colours
- G01J3/28—Investigating the spectrum
- G01J3/2823—Imaging spectrometer
Abstract
A kind of high-velocity scanning confocal imaging system available for spectrum analysis, is mainly used in biomedical micro-imaging field and investigation of materials, and sample is scanned by column by the burnt slit of copolymerization and single shaft scanning galvanometer, and with the face gust each row Confocal Images of detector acquisition；Meanwhile, by dispersion element and focus-compensating element by each column image, wavelength dispersion light beam is pressed on the vertical direction of slit is focused on together, face battle array detector is obtained the spectral information of each row image.Finally, by computer reconstructed picture, Confocal Images complete, with spectral information are obtained.
Description
Technical field
The present invention relates to a kind of high-velocity scanning confocal imaging system available for spectrum analysis, the more particularly to spectrum analysis for the three-dimensional co-focusing imaging of sample.Present invention is mainly applied to biomedical micro-imaging field, it can also be used to investigation of materials.
Background technology
With the development that spectrum analysis is applied in RESEARCH ON CELL-BIOLOGY, scientist is in the urgent need to when obtaining high-resolution Confocal Images, while obtaining the spectral information in each site in image.But, only the simple scan confocal imaging system of point by point scanning can once obtain the spectrum picture in a site by dispersion element at present, and then point by point scanning sample is to obtain Confocal Images complete, with spectral information, and imaging takes very much.Therefore, simple scan confocal imaging system is dfficult to apply to obtain imaging at a high speed.
And for the rotating disk confocal imaging system of high-velocity scanning, due to the array that the pin hole of enormous amount is planar alignment, system can only gradually pass through the light of different wave length by filter elements such as liquid crystal filter mirrors, then recombinated by computer, to obtain Confocal Images complete, with spectral information.Therefore, the rotating disk confocal imaging system for spectrum analysis can lose substantial amounts of optical signal, and spectral resolution is higher, and loss is bigger.
The content of the invention
The purpose of the present invention, is to scan by column sample using the burnt slit of copolymerization and single shaft scanning galvanometer, to realize three-dimensional co-focusing imaging；Light beam is pressed by wavelength dispersion on the vertical direction of slit is focused on together by dispersion element simultaneously, obtain all spectrum pictures of each column Confocal Images simultaneously with face battle array detector, Confocal Images complete, available for spectrum analysis are then reassembled as by computer.
The purpose of the present invention is realized by following technical scheme：
As shown in figure 1, a kind of high-velocity scanning confocal imaging system available for spectrum analysis, including：Light source 1, linear lighting device excites optical filtering 4, dichroic beam splitter 5, copolymerization Jiao's slit 6, the first telecentric imaging lens group 7, single shaft scanning galvanometer 8, microscope 9, second telecentric imaging lens group 10, dispersion element, focus-compensating element 12, face battle array detector 13, the (not shown) of controller 14 and the (not shown) of computer 15.It is characterized in that：
The light that light source 1 is launched is after linear lighting device (including first convex cylindrical mirror 3 of convex cylindrical mirror 2 and second), it is shaped as focusing on the burnt slit 6 of copolymerization on the vertical direction of slit 6 is focused on together, is parallel linear collimated light on the parallel direction of slit 6 is focused on together；
Optical filtering 4 is excited through the linear collimated light of specific band, by the light of other wave bands；
Dichroic beam splitter 5 reflects the light of other wave bands through the linear collimated light of the specific band；
The direction of copolymerization Jiao's slit 6 is overlapped with the direction of the linear collimated light；
First 7 groups of telecentric imaging lens are 1 times of multiplying power, and thing focal plane is located at the burnt slit 6 of copolymerization, as focal plane is overlapped with the imaging focal plane of microscope 9；
Single shaft scanning galvanometer 8 is located at the center of the first telecentric imaging lens group 7, and the direction that rotary shaft focuses on slit 6 together is parallel；
Second telecentric imaging lens group 10 is 1 times of multiplying power, and thing focal plane is located at the burnt slit 6 of copolymerization, as focal plane is located at face battle array detector 13；
The light that dispersion element 11 will transmit through the second telecentric imaging lens group 10 spatially turns into a plurality of light beam by wavelength dispersion, and the direction that its dispersion direction focuses on slit 6 together is vertical, and it is parallel that rotary shaft focuses on slit 6 together；
The cross section of focus-compensating element 12 is uniform thickness circular arc, and circular arc direction is consistent with the dispersion direction of the dispersion element, and center of arc is located at dispersion starting point；The refractive index of focus-compensating element 12 is constant, or along the dispersion direction gradual change of dispersion element 11.
The pixel arrangement of face battle array detector 13 focuses on the direction of slit 6 together respectively and the dispersion direction of dispersion element 11 is overlapped；
Switch, the swing of single shaft scanning galvanometer 6, the switch of face battle array detector 13 of the control light source 1 of controller 14；
The face signal that detects of battle array 13 devices of detection and is reassembled as including the Confocal Images of spectral information by calculator 15 as the host computer of controller 14.
Wherein, dispersion element is grating 11 or prism 22 (shown in Fig. 2).
The linear lighting device is made up of the convex cylindrical mirror 2 of focus direction orthogonal first, the second convex cylindrical mirror 3；Or as shown in Figure 3 and Figure 4 by collimating mirror 17, the first cylindrical mirror 18, the second convex cylindrical mirror 3 that focus and focus direction are overlapped, and focus direction focuses on the 3rd vertical projection face microscope group of slit 6 in groups into the first cylindrical mirror 18 is recessed cylindrical mirror or convex cylindrical mirror together；Or as shown in Figure 5 by collimating mirror 17, the first right-angle prism 19, the second right-angle prism 20, and focus direction focus on together the 3rd vertical projection face microscope group of slit 6 in groups into.
Advantages of the present invention is as follows：
1st, the present invention is by the burnt slit of copolymerization and the oscillatory scanning sample of single shaft scanning galvanometer, and co-focusing imaging speed is fast.
2nd, the present invention is being focused on the vertical direction of slit to light beam dispersion together by dispersion element, and the spectral information of single-row Confocal Images can be obtained simultaneously.
3rd, the present invention realizes the spectral scan function of multi-point scanning (slit scan).
Brief description of the drawings
Fig. 1：The schematic diagram of the first embodiment of the present invention
Fig. 2：The schematic diagram of second of embodiment of the present invention
Fig. 3：The schematic diagram of the third embodiment of the present invention
Fig. 4：The schematic diagram of the 4th kind of embodiment of the present invention
Fig. 5：The schematic diagram of the 5th kind of embodiment of the present invention
Fig. 6：The scanning imagery mode of system
Fig. 7：The imaging schematic diagram of system
Drawing explanation：
1 --- light source；2 --- the first convex cylindrical mirror；3 --- the second convex cylindrical mirror；4 --- excite optical filtering；5 --- dichroic beam splitter；6 --- copolymerization Jiao's slit；7 --- the first telecentric imaging lens group；8 --- single shaft scanning galvanometer；9 --- microscope；10 --- the second telecentric imaging lens group；11 --- grating；12 --- focus-compensating element；13 --- face battle array detector；16 --- sample；17 --- collimating mirror；18 --- the first cylindrical mirror；19 --- the first right-angle prism；20 --- the second right-angle prism；21 --- the 3rd convex cylindrical mirror；22 --- prism.
Embodiment
The present invention is further described with reference to the accompanying drawings and examples.
Embodiment 1
Fig. 1 is the schematic diagram of the first confocal optical scanner related to the present invention, and mode of operation is as follows：
The (not shown) of computer 15 sends a signal to the (not shown) of controller 14, and control light source 1 sends diverging light, while control detector 13 starts exposure.
Diverging light is focused on and focuses on slit 6 together by the first convex cylindrical mirror 2 on the vertical direction of slit 6 is focused on together, then diverging light is focused to directional light by the second convex cylindrical mirror 2 on the parallel direction of slit 6 is focused on together, and the parallel linear collimated light of slit 6 is focused on together.Linear collimated light is located at the sample 16 on the focal plane of microscope 9 through exciting after the burnt slit 6 of optical filtering 4, dichroic beam splitter 5 and copolymerization by the first telecentric imaging lens group 7, single shaft scanning galvanometer 8 and the projection illumination of microscope 9.Sample 16 returns to the light come and reflected after microscope 9, first yuan of telecentric imaging lens group 7, single shaft scanning galvanometer 8 and the burnt slit 6 of copolymerization by dichroic beam splitter 5, then it is imaged and face battle array detector 13 by the second telecentric imaging lens group 10 again, and the grating 11 by being placed on behind the second telecentric imaging lens group 10, the image of the burnt slit 6 of copolymerization is pressed into wavelength dispersion on the vertical direction of slit 6 is focused on together, the light beam of different wave length is then focused on into face battle array detector 13 (as shown in Figure 6) by focus-compensating element 12.
Detector 13 terminates exposure, and rank image transmitting by first and give the (not shown) of computer 15, the (not shown) of controller 14 control light source 1 is closed, then control the single shaft scanning galvanometer 8 to be rocked to the next position, carry out second and rank imaging ... until completion is all to rank imaging.Finally, all image reorganizations of ranking are the Confocal Images with spectral information by the (not shown) of computer 15, as shown in Figure 7.
Embodiment 2
Fig. 2 is the partial schematic diagram of second of high-velocity scanning confocal imaging system related to the present invention, and the main distinction of itself and embodiment 1 is as follows：The light splitting of grating 11 is substituted using prism 22.
Embodiment 3
Fig. 3 is the partial schematic diagram of second of high-velocity scanning confocal imaging system related to the present invention, and the main distinction of itself and embodiment 1 is as follows：The light that light source 1 is launched is collimated by collimating mirror 17, then expanded by the first convex cylindrical mirror 18 of short focus with the second convex cylindrical mirror 3 of long-focus on the parallel direction of focusing slit 6 together, then diverging light is focused on into focusing slit 6 together on the vertical direction of slit 6 is focused on together by the 3rd convex cylindrical mirror 21.
Embodiment 4
Fig. 4 is the partial schematic diagram of second of high-velocity scanning confocal imaging system related to the present invention, and the main distinction of itself and embodiment 1 is as follows：The light that light source 1 is launched has collimating mirror 17 to collimate, then expanded by the first recessed cylindrical mirror 18 of short focus with the second convex cylindrical mirror 3 of long-focus on the parallel direction of focusing slit 6 together, then diverging light is focused on into focusing slit 6 together on the vertical direction of slit 6 is focused on together by the 3rd convex cylindrical mirror 21.
Embodiment 5
Fig. 5 is the partial schematic diagram of second of high-velocity scanning confocal imaging system related to the present invention, and the main distinction of itself and embodiment 1 is as follows：The light that light source 1 is launched has collimating mirror 17 to collimate, then successively reflected by the first right-angle prism 19 and the second right-angle prism 20, expanded on the parallel direction of slit 6 is focused on together, then diverging light is focused on into focusing slit 6 together on the vertical direction of slit 6 is focused on together by the 3rd convex cylindrical mirror 21.
Claims (6)
1. a kind of high-velocity scanning confocal imaging system available for spectrum analysis, including：Light source, linear lighting device excites optical filtering
Mirror, dichroic beam splitter, copolymerization Jiao slit, the first telecentric imaging lens group, single shaft scanning galvanometer, microscope, the second telecentricity into
As lens group, dispersion element, focus-compensating element, face battle array detector, controller and computer.It is characterized in that：
The light of the light source transmitting is shaped as on the direction vertical with the burnt slit of the copolymerization after the linear lighting device
The burnt slit of the copolymerization is focused on, is parallel linear collimated light on the direction parallel with the burnt slit of the copolymerization；
The linear collimated light for exciting optical filtering to pass through specific band, by the light of other wave bands；
The dichroic beam splitter passes through the linear collimated light of the specific band, reflects the light of other wave bands；
The direction of the burnt slit of the copolymerization is overlapped with the direction of the linear collimated light；
The first telecentric imaging lens group is 1 times of multiplying power, and thing focal plane is located at the burnt slit of the copolymerization, as focal plane with it is described micro-
The imaging focal plane of mirror is overlapped；
The single shaft scanning galvanometer is located at the center of the first telecentric imaging lens group, rotary shaft and the side of the burnt slit of the copolymerization
To parallel；
The second telecentric imaging lens group is 1 times of multiplying power, and thing focal plane is located at the burnt slit of the copolymerization, as focal plane is located at the face
Battle array detector；
The light that the dispersion element will transmit through the second telecentric imaging lens group spatially turns into a plurality of light beam by wavelength dispersion,
Its dispersion direction is vertical with the direction of the burnt slit of the copolymerization, and rotary shaft is parallel with the burnt slit of the copolymerization；
The cross section of the focus-compensating element is uniform thickness circular arc, and circular arc direction is consistent with the dispersion direction of the dispersion element, circle
Arc is centrally located at dispersion starting point；
The pixel arrangement of face battle array detector direction respectively with the burnt slit of the copolymerization and the dispersion direction weight of the dispersion element
Close；
The controller controls the switching of the light source, the swing of the single shaft scanning galvanometer, the switch of face battle array detector；
The face signal that detects of battle array detector and is reassembled as including by the calculator as the host computer of the controller
The Confocal Images of spectral information.
2. a kind of high-velocity scanning confocal imaging system available for spectrum analysis according to claim 1, it is characterised in that：
Described dispersion element is prism or grating.
3. a kind of high-velocity scanning confocal imaging system available for spectrum analysis according to claim 1 or 2, its feature exists
In：The refractive index of the focus-compensating element is constant, or along the dispersion direction gradual change of the dispersion element.
4. a kind of high-velocity scanning confocal imaging system available for spectrum analysis according to claims 1 to 3 any one,
It is characterized in that：The linear lighting device by the convex cylindrical mirror of focus direction orthogonal first, the second projection face microscope group into.
5. a kind of high-velocity scanning confocal imaging system available for spectrum analysis according to claims 1 to 3 any one,
It is characterized in that：The first cylindrical mirror, the second convex cylindrical mirror that the linear lighting device is overlapped by collimating mirror, focus and focus direction,
And vertical the 3rd projection face microscope group of the burnt slit of focus direction and the copolymerization into；First cylindrical mirror is recessed cylindrical mirror or
Two convex cylindrical mirrors.
6. a kind of high-velocity scanning confocal imaging system available for spectrum analysis according to claims 1 to 3 any one,
It is characterized in that：The linear lighting device is by collimating mirror, the first right-angle prism, the second right-angle prism, and focus direction and institute
State the 3rd vertical projection face microscope group of the burnt slit of copolymerization into.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN201610221778.4A CN107290286A (en) | 2016-04-12 | 2016-04-12 | A kind of high-velocity scanning confocal imaging system available for spectrum analysis |
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| CN201610221778.4A CN107290286A (en) | 2016-04-12 | 2016-04-12 | A kind of high-velocity scanning confocal imaging system available for spectrum analysis |
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| CN110596059A (en) * | 2019-09-05 | 2019-12-20 | 北京世纪桑尼科技有限公司 | Optical super-resolution microscopic imaging system |
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