CN107281540A - Treat the Nano Silver temperature-sensitive hydrogel and preparation method of burn and scald - Google Patents
Treat the Nano Silver temperature-sensitive hydrogel and preparation method of burn and scald Download PDFInfo
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- CN107281540A CN107281540A CN201610220023.2A CN201610220023A CN107281540A CN 107281540 A CN107281540 A CN 107281540A CN 201610220023 A CN201610220023 A CN 201610220023A CN 107281540 A CN107281540 A CN 107281540A
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Abstract
The invention discloses a kind of Nano Silver temperature-sensitive hydrogel for treating burn and scald, the raw material for removing row parts by weight is prepared from：Chitosan 120 160；Silver nitrate 0.06 0.09；Sodium glycero-phosphate 8 12；Flamazine 19 25；Propolis 14 18；Dibutyl phthalate 22 26；Sodium alginate 18 24；Viola japonica 15 23；Scorpio 9 17；Centella 16 20；Marasmius equicrinis Muell 14 19；Glutinous rehmannia 6 11；And oneself 5 10.The excellent results of the present invention are also manifested by：Chitosan nano silver composite is made with solid phase method in the present invention, and load kinds of traditional Chinese medicines raw material, prepare the Nano Silver temperature-sensitive hydrogel for the treatment of burn and scald, the characteristics of there is Nano Silver and chitosan simultaneously and advantage, not only possesses nanometer silver antimicrobial, it is also equipped with chitosan and promotes cell growth, reduces the effect of scar generation.
Description
Technical field
The present invention relates to medical material field, more particularly to a kind of Nano Silver temperature-sensitive hydrogel and system for treating burn and scald
Preparation Method.
Background technology
Chitosan is the alkaline polysaccharide that nature is uniquely largely present, and is one kind sun that chitin is formed after deacetylated
Nonionic polysaccharide.Chitosan is widely present in the cell membrane of the shell of arthropod, fungi and yeast, and stock number is enriched very much,
There is the characteristic such as biodegradable, nontoxic, antibacterial simultaneously.There is substantial amounts of hydroxyl and amino, Yi Beihua in chitosan molecule
Modification is learned, complex can be combined to form with metal ion, a series of new chitosan derivatives is formed, is provided with more biologies
Function is learned, therefore has obtained in-depth study in medical science, field of biology and has been widely applied.
Silver ion in early stage just by its excellent antibiotic and sterilizing performance is by people institute extensive concern, its medical material with
The fields such as anti-biotic material have broad application prospects.There is no obvious cell to gather with the cell after low concentration silver ion treatment
The change of collection, cytomorphosis, cell dissolving etc..A small amount of silver ion does not have obvious injury effect to human body as can be seen here, together
Shi Yin has good bactericidal action.Nano Silver is the novel antibacterial product developed based on nanometer technology, compares Ag +Tool
There is more stable physicochemical characteristics, Nano Silver is not likely to produce drug resistance due to quantum effect, small-size effect, accelerates wound
Healing, had broad application prospects in the field such as medical material and anti-biotic material.
Temperature sensitive type water gel refers to the intellectual material that stimulating responsive is produced with the change of ambient temperature.Temperature
There is a certain proportion of hydrophilic and hydrophobic grouping, the change of temperature can influence dredging for these groups in sensitive hydrogel structure
Water acts on the hydrogen bond action between macromolecular chain, so as to influence its metamorphosis.Most common temperature-sensitive hydrogel is poly- N-
Existing hydrophobic grouping has hydrophilic radical again in isopropylacrylamide hydrogel, the system of this hydrogel, and these groups can be with
Act on, also had an effect outside molecule with hydrone in intramolecular and hydrone.Compared with synthesizing macromolecule, natural polymer coagulates
Glue has the advantages that hypotoxicity, biocompatibility and degradability.Thermo-sensitive aquagel can be according to temperature change
Different physical aspects.Under normal temperature be liquid, into after tissue, body temperature effect under gelation and be fixed on local organization
In.Chitosan only keeps solution state under sour environment, and when pH value is increased to 6.2, chitosan solution starts to sink
Form sediment, if adding sodium glycero-phosphate, the system can keep solution state in neutral conditions, and the system has temperature sensitivity,
Solution state is kept below body temperature, gel can be formed when temperature is increased to body temperature.When it has good biocompatibility, degraded in vivo
Between and setting time the advantages of be adjustably controlled, lead bioengineering, field of medicaments to obtain extensive concern in engineering etc..
The content of the invention
It is an object of the invention to provide a kind of Nano Silver temperature-sensitive hydrogel and preparation method for treating burn and scald, the water-setting
Glue has a broad antifungal spectrum, no cytotoxicity, high-efficiency low-toxicity；Can be in wound surface formation layer of gel film, antibacterial work after uniform sprinkling
It is good with lasting, gas permeability and hygroscopicity, and the effect sterilized with wound healing, hemostasis, analgesic, anti-inflammatory.
The technical scheme is that：
The Nano Silver temperature-sensitive hydrogel of burn and scald is treated, the raw material for removing row parts by weight is prepared from：Chitosan 120-160；Nitre
Sour silver 0.06-0.09；Sodium glycero-phosphate 8-12；Flamazine 19-25；Propolis 14-18；Dibutyl phthalate 22-26；
Sodium alginate 18-24；Viola japonica 15-23；Scorpio 9-17；Centella 16-20；Marasmius equicrinis Muell 14-19；Glutinous rehmannia 6-11；And own 5-10.
It is preferred that scheme be：Chitosan 130-150；Silver nitrate 0.07-0.08；Sodium glycero-phosphate 9-11；Flamazine
21-23；Propolis 15-17；Dibutyl phthalate 23-25；Sodium alginate 20-22；Viola japonica 17-21；Scorpio 11-15；Product
Avenge grass 17-19；Marasmius equicrinis Muell 15-18；Glutinous rehmannia 7.5-9.5；And own 6-9.
More preferred：Chitosan 140；Silver nitrate 0.075；Sodium glycero-phosphate 10；Flamazine 22；Propolis 16；It is adjacent
Dibatyl phithalate 24；Sodium alginate 21；Viola japonica 19；Scorpio 13；Centella 18；Marasmius equicrinis Muell 17；Glutinous rehmannia 8.5；And oneself
7.5。
The preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is treated, step is as follows：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 7-11 minutes（It is preferred that slow fire heating is turned over
Fry 8-10 minutes）, then drench into the 0.25-0.45 times of sesame oil measured（It is preferred that drenching into the 0.3-0.4 times of sesame oil measured）, continue to stir-fry to
Medicinal material surface slightly has blackspot, take out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in Chinese medicament extracting device, with 4.5-7.5
The ethanol of the raw material dose is solvent, refluxing extraction 1-3 hours again（It is preferred that refluxing extraction 1.5-2.5 hours）, collect alcohol extracting
Liquid, filtering reclaims ethanol, obtains the concentrated liquid of tradition Chinese medicine, standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 2-5 hours is placed in grinder
（Quick grinding 3-4 hours is preferably disposed in grinder）, through ultraviolet light 10-18 hours, chitosan nano silver composite is obtained,
It is standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano that mass concentration is 1%-2% with diluted acid
Silver composite solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 30- is warming up to
48 DEG C are stirred 18-30 minutes（Stirred 22-26 minutes it is preferred that being warming up to 36-42 DEG C）, then pH adjusted dose adjusts pH to 6.0-
7.0；
（5）Will（4）The solution of gained is put into 35 DEG C of -38 DEG C of thermostat water baths（It is preferably placed into 36 DEG C of -37 DEG C of thermostat water baths
In）, phase in version occurs after 2-6 minutes, immobilising hydrogel is changed into by flowable colloidal solution.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（1）The amount of ethanol is the raw material
5.5-6.5 times of dose（The amount of preferred alcohol is 6 times of the raw material dose）.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（2）Ultraviolet light 12-16 is small
When（It is preferred that ultraviolet light 14 hours）.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（3）The diluted acid is 0.08-
0.16mol/L aqueous citric acid solution（It is preferred that the diluted acid be 0.1-0.14mol/L aqueous citric acid solution, more preferably
The diluted acid be 0.12mol/L aqueous citric acid solution）.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（3）Being dissolved into mass concentration is
1.3%-1.7% chitosan nano silver complex solution（It is combined it is preferred that being dissolved into the chitosan nano silver that mass concentration is 1.5%
Thing solution）.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（4）PH adjusting agent is phosphoric acid hydrogen two
Sodium.
The preparation method of above-mentioned Nano Silver temperature-sensitive hydrogel, scheme preferably is, step（4）Adjust pH to 6.3-6.7（It is excellent
Recruit section pH to 6.5）.
The pharmacological effect or physicochemical property of each bulk drug be respectively：
Chitosan：Chitosan is the product that chitin takes off N- acetyl group, it is however generally that, N- acetyl group slough more than 55% can
Referred to as chitosan, in other words, can dissolve 1% chitosan, this chitosan in 1% acetic acid or 1% hydrochloric acid
It is referred to as chitosan.Physiologically active：1st, cholesterol is controlled；2nd, bacterial activity is suppressed：Chitosan is easy to molten in weak acid solvent
Solution, especially it is worthy of note that dissolving after solution in contain amino（NH2+）, these amino with reference to negatron by suppressing thin
Bacterium.The suppression bacterial activity of chitosan, makes it be had a wide range of applications in fields such as medicine, weaving and food.3rd, prevent and control
Hypertension processed；4th, adsorb and drain heavy metal；5th, immune effect：Chitosan has higher protein adsorption ability；In digestive enzyme
（Lysozyme）In the presence of, chitosan tool degradability；Chitosan is easy to be processed into line, is adapted to the doctor for making wire or sheet
Use material；Chitosan has affinity and dissolubility, it is adaptable to produce each analog derivative；Chitosan has higher chemistry living
Property；The retentiveness of chitosan is high；In serum, chitosan is degradable to be absorbed；Chitosan has higher biological degradability；Shell gathers
Sugar shows the effect that selectively height suppresses Streptococcus oralis growth, while having no effect on the life of other beneficial bacterias
It is long.
Silver nitrate：Water white transparency orthorhombic system flat crystal, soluble in water and ammoniacal liquor is dissolved in ether and glycerine, is slightly soluble in
Absolute ethyl alcohol, is practically insoluble in concentrated nitric acid.Its aqueous solution is in faintly acid.Silver nitrate solution is due to containing a large amount of silver ions, therefore oxygen
The property changed is stronger, and has certain corrosivity, medically for the granulation tissue for corroding hyperplasia, and weak solution is used for the sterilization of ocular infection
Agent.It is light yellow liquid after fusing, is still white after solidification.Purposes：Analytical chemistry is used to precipitate chlorion, the nitre of working standard
Sour silver is used to demarcate sodium chloride solution.Inorganic industrial is used to manufacture other silver salt.Electronics industry is used to manufacturing electroconductive binder, new
Type gas purifying agent, A8x molecular sieves, silver-plated gloves forced to submit with livewire work etc..It is photosensitive industry be used for manufacture cinefilm,
The photosensitive material of x light photographic negative and photographic film etc..Electroplating industry is used for the silver-plated of electronic component and other handicrafts, also greatly
Silver coating material of the amount as mirror and glass liner of thermos.Battery industry is used to produce smee cell.Pharmaceutically it is used as bactericide, corrosion
Agent.Daily chemical industry is used for dyeing hair etc..It is used to determine chlorine, bromine, iodocyanide and rhodanate in analytical chemistry.
Phosphoglycerol is received：For colourless crystallization or white crystals sprills；Odorless, taste is salty.This product is readily soluble in water, in ethanol
Or it is insoluble in acetone.Simple chitosan only keeps solution state under sour environment, when pH value is increased to 6.2, chitosan
Solution starts to precipitate, but in the presence of weak base sodium glycero-phosphate, the system can keep stable solution in neutral conditions
State, this is that the protein-based medicine of the system loads even cell living is provided convenience condition.It is finally that the system has
Temperature-sensing property, is in solution state below room temperature, temperature is increased to curable formation gel during body temperature, so as to be sustained loading
The effect of medicine.
Flamazine：It is a kind of sulfamido/silver salt antibacterium medicine, is the crystalline powder of white or off-white color.For
Empyrosis wound surface infection is treated, in addition to infection control, can also promote the surface of a wound to dry, form a scab and promote healing.With sulphadiazine
Antibacterial action and silver salt astriction, there is powerful inhibitory action to Pseudomonas aeruginosa, it is stronger than Homosulfamine.Has a broad antifungal spectrum,
There is good antibacterial activity to most gram positive bacterias and negative bacterium, antibacterial action is not by PABA in fester（Aminobenzoic acid）'s
Influence；Resisting pseudomonas aeruginosa effect is significantly stronger than mafenide.For prevent and treat II degree, III degree burn or scald after
The trauma surface infestation of hair.
Propolis：It is the resin that honeybee gathers from plant gemma or trunk, is mixed into the secretion of palatine gland, wax gland thereon
A kind of colloidal solid thing with aromatic odor processed.It is secreted by the repairing honeycomb such as Apidae animal apis cerana
The stickum of yellowish-brown or dark brown, can be used as medicine.Its is mild-natured, bitter, pungent, micro-sweet, there is moisturizing myogenic, the work(of anti-inflammatory analgetic
Effect, can treat gastric ulcer, canker sore, burn and scald, skin Tearing Pain, the illness such as radiation proof.Primary efficacy：It is anti-that propolis has
Bacterium, beautifying face and moistering lotion, promote cytothesis effect, it can not only eliminate fungal infection, additionally it is possible to decompose pigment, calm down wrinkle,
Delaying senility, accelerates histiocytic regeneration and the healing of wound, also has good curative effect to the left acne scar acne print of small pox.
Sodium alginate：It is a kind of natural polysaccharide, with the stability needed for pharmaceutical preparation auxiliary material, dissolubility, viscosity and peace
Quan Xing.Film is can be made into, for candy release packaging.Available for increase blood volume and maintenance blood pressure, exclude produced by burning
Histamine toxoid and wound are lost blood, the stabilization of the perioperatively circulatory system, bleeding profusely property shock, burn shock, high fever
With the dehydration of the whole body such as acute dysentery, therapeutic effect is good.Also having excretes cholesterol, suppresses Pb, Cd, Sr and is inhaled by human body
Receive and protection intestines and stomach, whole intestines, fat-reducing, hypoglycemic effect.Be mainly used as on medicament suspending agent, emulsifying agent, sticky dose,
Capsule material of micro-capsule etc..Mosanom is a kind of high-molecular compound of high viscosity.The difference of it and starch, cellulose etc., be
It has carboxyl, is the high glycan aldehydic acid that the aldehyde radical of beta-D-mannuronic acid is formed with glycosidic bond.Its functional character is as follows：（1）Parent
It is aqueous strong, it can be dissolved in cold water and warm water, form very sticky uniform solution.（2）The true solution of formation has it
His analog is difficult to flexibility, homogeneity and other good characteristics obtained.（3）Effect with very strong protecting colloid, it is right
The emulsifying ability of grease is strong.（4）The metal salts such as aluminium, barium, calcium, copper, iron, lead, zinc, nickel are added in the solution, will be generated insoluble
Alginates.These metal salts are the buffers of sodium, the phosphate of potassium, acetate etc., can be suppressed, retarded coagulation.（5）Generally
PH value is 7, in neutrality, can be adjusted between 4-10.（6）It is wire polyelectrolyte, can be made into tough fiber（Alginic acid
Fiber）Or film, acted on cracking is prevented.（7）Adherence is small, it is adaptable to warp size, demoulding etc..
Viola japonica：Ji Yuan：For the herb or root of Violaceae plant viola japonica.Nature and flavor：Bitter；Micro-pungent；It is cold in nature.Channel tropism：Liver；
The spleen channel.Major function：It is clearing heat and detoxicating；Stagnation resolvation apocenosis；Cool blood clears liver.Main swollen ulcer drug；Acute mastitis；Blood under acute appendicitis；Suppurative marrow
It is scorching；Jaundice；Red eye, swell pain；Scrofula；Traumatism and bleeding；Snakebite.
Scorpio：Ji Yuan：This product is the hirudo leech of Buthidae animal buthus martensii Karscs.Nature and flavor：It is pungent, put down；It is poisonous.Channel tropism：Return liver
Through.Effect：Ceases wind antispastic, dispersing pathogen accumulation is removed obstruction in channels to relieve pain.Cure mainly：For child convulsion, tic spasm, hemiplegia breaks wound
Wind, rheumatoid arthritis stubborn, migraine and general headache, sore, scrofula.
Centella：Ji Yuan：For the drying herb or whole herb with root of dicotyledon Umbelliferae centella.Composition：Containing a variety of
The Triterpenoid of α-botany bar gum alcohol type, wherein have asiaticosid, thankuniside, isothankuniside, madecassoside, glass hot-die
Glycosides, broken hot rice glycosides and Brahmic acid etc., and madasiatic acid.The property of medicine：Bitter, acrid, cold.Return liver, spleen, kidney channel.Function is cured mainly：
It is clearing heat and detoxicating, it is inducing diuresis and reducing edema.The long-lived medicine of Asians, can beneficial brain refresh oneself.Research show have nourishing, anti-inflammatory, healing of wound,
Diuresis defaecation and calm effect.It is also effective in cure to leprosy, ulcer, there is activation to blood purification and immunity, because it can
Stimulate the replacement of deep skin cell.It is neural nourishing agent, can improve memory, mitigates mental fatigue；Can also hypotensive, control
Treat hepatopathy etc..
Marasmius equicrinis Muell：Mycophyta Basidiomycetes Agaricales Agaricaceae Marasmius equicrinis Muell, is used as medicine with shoestring.For Bai Moke fungies, CNNS is small
Skin agaricin.Chemical analysis：Containing mannitol in marasmius androsaceus silk, shoestring, cholesteryl acetate, glycine, aspartic acid,
Threonine, valine etc. also isolates cupreol from mycelium, palmitic acid, 28 carbonic acid, hydroxycinnamic acid and small
Wheat flavine, separately containing humic acid.Nature and flavor：Slight bitter；It is warm-natured.Channel tropism：Return liver warp.Major function：Promoting blood circulation analgesic therapy.Main traumatic injury；Bone
Roll over pain；Antimigraine, various neuralgias, lumbocrural pain, arthralgia pain due to rheumatism.
Glutinous rehmannia：Ji Yuan：This product is the fresh or dried root of scrophulariaceae rehmannia glutinosa plant.Radix rehmanniae recen：It is in irregular group more
Block or Long Circle, centre is expanded, and two ends are slightly thin, what is had tiny, strip, slightly flat and distort.Nature and flavor：Fresh rehmannia root is sweet, hardship,
It is cold.Radix rehmanniae recen：Nature and flavor：It is sweet, tremble with fear.Channel tropism：The thoughts of returning home, liver and kidney channel.Effect：Fresh rehmannia root clearing heat and promoting fluid, cool blood, hemostasis.Cure mainly：With
In consumption of YIN caused by febrile disease, the deep red polydipsia of tongue.Spot dermexanthesis, spits blood, bleeding from five sense organs or subcutaneous tissue.Abscess of throat.Radix rehmanniae recen：Effect：Clearing heat and cooling blood, yin-nourishing is raw
Tianjin.For the deep red polydipsia of pyreticosis tongue, yin asthenia generating intrinsic heat, hectic fever due to yin labor heat, Heat Diabetes is spitted blood, rugged blood, spot dermexanthesis.
And oneself：Ji Yuan：For Chloranthaceae plant and oneself root.Chemical analysis：Herb is containing pyrocurcuzerenone, the burnt cowherb of left-handed dihydro
Art ketone.Nature and flavor：" bitter, puts down, poisonous." channel tropism：Liver Channel.Major function：Promoting blood circulation to remove blood stasis.Control traumatic injury, sore scabies, furuncle, the moon
Through apolipsis.Desinsection.For traumatic injury, nameless sores or boils, favus of the scalp, white bald, pruitus, amenorrhoea；Kill maggot and wriggler.
The Nano Silver temperature-sensitive hydrogel scientific formula for the treatment burn and scald that the present invention is provided, preparation method is simple,.Except this it
Outside, excellent results of the invention are also manifested by：
1、Chitosan nano silver composite is made with solid phase method in the present invention, and loads kinds of traditional Chinese medicines raw material, prepares treatment burn and scald
Nano Silver temperature-sensitive hydrogel, while the characteristics of having Nano Silver and chitosan and advantage, not only possess nanometer silver antimicrobial, also
Possess chitosan and promote cell growth, reduce the effect of scar generation；
2、Gained Nano Silver temperature-sensitive hydrogel of the invention has antibacterial, promotion wound healing, reduces scar generation, the work of hemostasis
With, and with good moisturizing, gas permeability, no cytotoxicity, good antimicrobial effect, persistent；
3、Gained Nano Silver temperature-sensitive hydrogel of the invention is high to the treatment rate of burn and scald, can preferably prevent trauma surface infestation, may be used also
To reduce the use of antibiotic, and preparation technology is simple, has broad application prospects.
Embodiment
Technical scheme is described in detail with reference to embodiment and experimental example, but protection domain is not limited by this.
Embodiment 1 treats the Nano Silver temperature-sensitive hydrogel of burn and scald, and the raw material for removing row parts by weight is prepared from（50g/
Part）：120 parts of chitosan；0.06 part of silver nitrate；8 parts of sodium glycero-phosphate；19 parts of flamazine；14 parts of propolis；Phthalic acid
22 parts of dibutyl ester；18 parts of sodium alginate；15 parts of viola japonica；9 parts of scorpio；16 parts of centella；14 parts of Marasmius equicrinis Muell；6 parts of glutinous rehmannia；And oneself
5 parts.
Treating the preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 7 minutes, then drench what is measured into 0.25 times
Sesame oil, continues to stir-fry to medicinal material surface slightly blackspot, takes out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in traditional Chinese medicine extraction
In device, the ethanol using 4.5 times of raw material doses is solvent, refluxing extraction 1 hour, collects alcohol extract, and ethanol is reclaimed in filtering,
The concentrated liquid of tradition Chinese medicine is obtained, it is standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 2 hours, warp is placed in grinder
Ultraviolet light 10 hours, obtains chitosan nano silver composite, standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano silver that mass concentration is 1% with diluted acid
Complex solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；The diluted acid is
0.08mol/L aqueous citric acid solution；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 30 are warming up to
DEG C stirring 18 minutes, then pH adjusted dose of disodium hydrogen phosphate regulation pH to 6.0；
（5）Will（4）The solution of gained is put into 35 DEG C of thermostat water baths, phase in version occurs after 6 minutes, by flowable colloid
Solution is changed into immobilising hydrogel.
Embodiment 2 treats the Nano Silver temperature-sensitive hydrogel of burn and scald, and the raw material for removing row parts by weight is prepared from（30g/
Part）：160 parts of chitosan；0.09 part of silver nitrate；12 parts of sodium glycero-phosphate；25 parts of flamazine；18 parts of propolis；O-phthalic
26 parts of dibutyl phthalate；24 parts of sodium alginate；23 parts of viola japonica；17 parts of scorpio；20 parts of centella；19 parts of Marasmius equicrinis Muell；11 parts of glutinous rehmannia；
And oneself 10 parts.
Treating the preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 11 minutes, then drench into 0.45 times of amount
Sesame oil, continue to stir-fry to medicinal material surface slightly blackspot, take out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in Chinese medicine and carry
Take in device, the ethanol using 7.5 times of raw material doses is solvent, refluxing extraction 3 hours, collect alcohol extract, second is reclaimed in filtering
Alcohol, obtains the concentrated liquid of tradition Chinese medicine, standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 5 hours, warp is placed in grinder
Ultraviolet light 18 hours, obtains chitosan nano silver composite, standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano silver that mass concentration is 2% with diluted acid
Complex solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；The diluted acid is
0.16mol/L aqueous citric acid solution；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 48 are warming up to
DEG C stirring 30 minutes, then pH adjusted dose of disodium hydrogen phosphate regulation pH to 7.0；
（5）Will（4）The solution of gained is put into 38 DEG C of thermostat water baths, phase in version occurs after 2 minutes, by flowable glue
Liquid solution is changed into immobilising hydrogel.
Embodiment 3 treats the Nano Silver temperature-sensitive hydrogel of burn and scald, and the raw material for removing row parts by weight is prepared from（45g/
Part）：130 parts of chitosan；0.07 part of silver nitrate；9 parts of sodium glycero-phosphate；21 parts of flamazine；15 parts of propolis；Phthalic acid
23 parts of dibutyl ester；20 parts of sodium alginate；17 parts of viola japonica；11 parts of scorpio；17 parts of centella；15 parts of Marasmius equicrinis Muell；7.5 parts of glutinous rehmannia；
And oneself 6 parts.
Treating the preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 8 minutes, then drench what is measured into 0.3 times
Sesame oil, continues to stir-fry to medicinal material surface slightly blackspot, takes out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in traditional Chinese medicine extraction
In device, the ethanol using 5.5 times of raw material doses is solvent, refluxing extraction 1.5 hours, collects alcohol extract, and second is reclaimed in filtering
Alcohol, obtains the concentrated liquid of tradition Chinese medicine, standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 3 hours, warp is placed in grinder
Ultraviolet light 12 hours, obtains chitosan nano silver composite, standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano that mass concentration is 1.3% with diluted acid
Silver composite solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；The diluted acid
For 0.1mol/L aqueous citric acid solution；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 36 are warming up to
DEG C stirring 22 minutes, then pH adjusted dose of disodium hydrogen phosphate regulation pH to 6.3；
（5）Will（4）The solution of gained is put into 36 DEG C of thermostat water baths, phase in version occurs after 5 minutes, by flowable colloid
Solution is changed into immobilising hydrogel.
Embodiment 4 treats the Nano Silver temperature-sensitive hydrogel of burn and scald, and the raw material for removing row parts by weight is prepared from（35g/
Part）：150 parts of chitosan；0.08 part of silver nitrate；11 parts of sodium glycero-phosphate；23 parts of flamazine；17 parts of propolis；O-phthalic
25 parts of dibutyl phthalate；22 parts of sodium alginate；21 parts of viola japonica；15 parts of scorpio；19 parts of centella；18 parts of Marasmius equicrinis Muell；Glutinous rehmannia 9.5
Part；And oneself 9 parts.
Treating the preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 10 minutes, then drench what is measured into 0.4 times
Sesame oil, continues to stir-fry to medicinal material surface slightly blackspot, takes out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in traditional Chinese medicine extraction
In device, the ethanol using 6.5 times of raw material doses is solvent, refluxing extraction 2.5 hours, collects alcohol extract, and second is reclaimed in filtering
Alcohol, obtains the concentrated liquid of tradition Chinese medicine, standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 4 hours, warp is placed in grinder
Ultraviolet light 16 hours, obtains chitosan nano silver composite, standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano that mass concentration is 1.7% with diluted acid
Silver composite solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；The diluted acid
For 0.14mol/L aqueous citric acid solution；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 42 are warming up to
DEG C stirring 26 minutes, then pH adjusted dose of disodium hydrogen phosphate regulation pH to 6.7；
（5）Will（4）The solution of gained is put into 37 DEG C of thermostat water baths, phase in version occurs after 3 minutes, by flowable colloid
Solution is changed into immobilising hydrogel.
Embodiment 5 treats the Nano Silver temperature-sensitive hydrogel of burn and scald, and the raw material for removing row parts by weight is prepared from（40g/
Part）：140 parts of chitosan；0.075 part of silver nitrate；10 parts of sodium glycero-phosphate；22 parts of flamazine；16 parts of propolis；O-phthalic
24 parts of dibutyl phthalate；21 parts of sodium alginate；19 parts of viola japonica；13 parts of scorpio；18 parts of centella；17 parts of Marasmius equicrinis Muell；Glutinous rehmannia 8.5
Part；And oneself 7.5 parts.
Treating the preparation method of the Nano Silver temperature-sensitive hydrogel of burn and scald is：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 6 minutes, then drench what is measured into 0.35 times
Sesame oil, continues to stir-fry to medicinal material surface slightly blackspot, takes out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in traditional Chinese medicine extraction
In device, the ethanol using 6 times of raw material doses is solvent, refluxing extraction 2 hours, collects alcohol extract, and filtering is reclaimed ethanol, obtained
The concentrated liquid of tradition Chinese medicine, it is standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 3.5 hours is placed in grinder,
Through ultraviolet light 14 hours, chitosan nano silver composite is obtained, it is standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano that mass concentration is 1.5% with diluted acid
Silver composite solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 39 are warming up to
DEG C stirring 24 minutes, then pH adjusted dose of disodium hydrogen phosphate regulation pH to 6.5；
（5）Will（4）The solution of gained is put into 36.5 DEG C of thermostat water baths, phase in version occurs after 4 minutes, by flowable glue
Liquid solution is changed into immobilising hydrogel.
The bacteriostasis experiment of the Nano Silver temperature-sensitive hydrogel of the present invention treatment burn and scald of test example 1
1 experiment material
Test sample：A samples：0.05% temperature-sensitive hydrogel containing Nano Silver；B samples：The 0.025% temperature sensitive water-setting containing Nano Silver
Glue；C sample：0.01% temperature-sensitive hydrogel containing Nano Silver；D samples：Chitosan temperature-sensitive hydrogel.
Culture medium：Beef-protein medium, sabouraud culture medium, blood serum medium.
Strains tested：（1）Bacterium：Pseudomonas aeruginosa ATCC27853, staphylococcus aureus ATCC25923, large intestine bar
Bacterium ATCC25922（There is provided by Taishan Hospital）, enterobacter cloacae（Purchase in the base biochemical technology Co., Ltd of Nanjing one）With
Streptococcus pneumonia（Purchase in Shanghai Bei Nuo bio tech ltd）.
（2）Fungi：Candida albicans ATCC10231（Purchase in Shanghai element that bio tech ltd）, smooth beads
Bacterium（Purchase in Nanjing Bian Zhen bio tech ltd）, acrothesium floccosum（Purchase in the upper limited public affairs of Haifeng county longevity biotechnology
Department）.
2. experimental method
Bacteriostasis of 2.1 each sample to 5 kinds of bacteriums
Each strain of preservation is subjected to activation culture respectively, grown after single bacterium colony, with a little thalline of oese picking in equipped with 9ml
In the test tube of sterile saline, shaken well prepares 10 respectively7 Cfu/ml suspension.Aseptically, 90mm's
Add appropriate culture medium in culture dish, 100 microlitres of bacterium solutions are added after solidification, coating is uniform, stands 20-30min.By the temperature sensitive of preparation
Hydrogel sample makes a diameter of 0.4cm circle respectively, is placed on solid medium, and shell is sequentially placed on same culture dish
Glycan is temperature sensitive, 0.01% temperature-sensitive hydrogel containing Nano Silver, 0.025% temperature-sensitive hydrogel containing Nano Silver, 0.05% containing Nano Silver
Temperature-sensitive hydrogel, is repeated 6 times, and 24h is cultivated in 37 DEG C of constant incubator；The contact surface of observation sample and culture medium whether there is carefully
Bacteria growing, and the size of inhibition zone is measured, analyzed using SPSS v11.5 software statistics, data are with mean ± standard deviation（x±S）
Represent.
Bacteriostasis of 2.2 each sample to 3 kinds of fungies
Each strain of preservation is subjected to activation culture respectively, it is 1 × 10 that concentration is prepared respectively6Cfu/ml bacterium solution, other operations
Ibid, 48h is cultivated in 37 DEG C of constant incubator, the contact surface for observing sample and culture medium whether there is bacterial growth, measures antibacterial
The size of circle.Analyzed using SPSS software statistics, measurement data is with mean ± standard deviation（x±S）Represent.
3 results and analysis
3.1 temperature-sensitive hydrogels containing Nano Silver are to 5 kinds of common bacterias：Pseudomonas aeruginosa ATCC27853, staphylococcus aureus
ATCC25923, Escherichia coli ATCC25922, enterobacter cloacae and the antibacterial circle diameter of streptococcus pneumonia are shown in Table 1.
The antibacterial antibacterial circle diameter of contact of each sample of table 1 to 5 kinds of bacteriums（Unit：cm；Sample diameter 0.4cm）
From the data in table 1, it can be seen that temperature-sensitive hydrogel and chitosan temperature-sensitive hydrogel containing Nano Silver are used to experiment
5 kinds of bacteriums are respectively provided with and preferably contact bacteriostasis, and the contact surface of sample and culture medium is without bacterial growth.With nanometer
The increase of silver content, fungistatic effect enhancing, illustrates that Nano Silver enhances the bacteriostasis of chitosan temperature-sensitive hydrogel.
3.2 each sample are to three kinds of fungies：Candida albicans ATCC10231, Candida glabrata, acrothesium floccosum connect
Touch antibacterial antibacterial circle diameter and be shown in Table 2.
The antibacterial antibacterial circle diameter of contact of each sample of table 2 to 3 kinds of fungies（Unit：cm；Sample diameter 0.4cm）
Temperature-sensitive hydrogel and chitosan temperature-sensitive hydrogel containing Nano Silver are respectively provided with preferable contact to experiment three kinds of fungies used
The contact surface of bacteriostasis, gel sample and culture medium grows without fungi, and with the increase of content of nanometer silver, bacteriostasis increases
By force.Temperature-sensitive hydrogel containing Nano Silver is most obvious to the bacteriostasis of Epidermophvton tinea, next to that Candida glabrata, to white thought
The bacteriostasis of pearl bacterium is markedly less than first two fungi.
4. result is with discussing
Experimental result is shown：Temperature-sensitive hydrogel containing Nano Silver is respectively provided with preferably to above-mentioned 5 kinds of bacterium bacterial strains and 3 kinds of fungal bacterial strains
Contact bacteriostasis.The bacteriostasis of the temperature-sensitive hydrogel containing Nano Silver is better than the bacteriostasis of chitosan temperature-sensitive hydrogel；It is right
The bacteriostasis of bacterium is better than the bacteriostasis to fungi；With the increase of content of nanometer silver, bacteriostasis enhancing, Nano Silver increases
The strong bacteriostasis of chitosan temperature-sensitive hydrogel.
The clinical practice of the Nano Silver temperature-sensitive hydrogel of the present invention treatment burn and scald of test example 2：
The burned patient totally 400 that in November, -2014 in July, 2012, our hospital accepted for medical treatment, wherein male 235 are chosen, women 165
Example, the age is in 7-59 Sui, depth of burn：Once burn and scald 200, two degree of burn and scalds 149, three degree of burn and scalds 51.It will suffer from
Person is randomly divided into experimental group and control group, and two groups of patients are statistically analyzed difference without aobvious in sex, the state of an illness, in terms of the age
Work property meaning（P ＞ 0.05）, with comparativity.Experimental group treats the temperature sensitive water-setting of Nano Silver of burn and scald using present invention gained
Glue, application method is：According to wound size, take and be uniformly applied to affected part, one day 2-3 times, 4 days courses for the treatment of in right amount.Observation altogether
Treat three courses for the treatment of.Control group uses other drugs（Such as ointment for treating burn and scald, jingwanhong soft plaster ointment for scald and pale reddish brown MEBO medicine）.Treat
Imitate criterion：Cure：Symptom disappearance, wound healing, without red and swollen, pigmentation；It is effective：Symptom disappear, wound healing 50% with
On, colour of skin mild hyperaemia；Effectively：Symptom substantially mitigates, wound healing more than 25%, and the colour of skin is congested substantially, heavy with slight pigment
；It is invalid：Symptom has no improvement, trauma surface infestation.Total effective rate=（Cure number of cases+effective number of cases+effective number of cases）/ total inspection disease
Number of cases × 100%.As a result it is as shown in table 3.
Table 3：Experimental group and the treatment results of control group compare
From the data in table 3, it can be seen that curing 163 in experimental group（67.92%）, effective 48（20%）, effective 26（10.83%）, nothing
Effect 3（1.25%）, total effective rate is 98.75%；And control group total effective rate is 81.25%, experimental group therapeutic effect is substantially better than
Control group.
Finally it should be noted that embodiment is the optimal embodiment of the present invention, this is not limited to
Invention, although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, its according to
The technical scheme described in foregoing embodiments can so be modified, or which part technical characteristic is equally replaced
Change.Within the spirit and principles of the invention, any modification, equivalent substitution and improvements made etc., should be included in the present invention
Protection domain within.
Claims (10)
1. treat the Nano Silver temperature-sensitive hydrogel of burn and scald, it is characterised in that the raw material for removing row parts by weight is prepared from：Shell gathers
Sugared 120-160；Silver nitrate 0.06-0.09；Sodium glycero-phosphate 8-12；Flamazine 19-25；Propolis 14-18；Phthalic acid
Dibutyl ester 22-26；Sodium alginate 18-24；Viola japonica 15-23；Scorpio 9-17；Centella 16-20；Marasmius equicrinis Muell 14-19；Glutinous rehmannia 6-
11；And own 5-10.
2. the Nano Silver temperature-sensitive hydrogel of burn and scald is treated as claimed in claim 1, it is characterised in that remove row parts by weight
Raw material is prepared from：Chitosan 130-150；Silver nitrate 0.07-0.08；Sodium glycero-phosphate 9-11；Flamazine 21-23；Honeybee
Glue 15-17；Dibutyl phthalate 23-25；Sodium alginate 20-22；Viola japonica 17-21；Scorpio 11-15；Centella 17-
19；Marasmius equicrinis Muell 15-18；Glutinous rehmannia 7.5-9.5；And own 6-9.
3. the Nano Silver temperature-sensitive hydrogel of burn and scald is treated as claimed in claim 1, it is characterised in that remove row parts by weight
Raw material is prepared from：Chitosan 140；Silver nitrate 0.075；Sodium glycero-phosphate 10；Flamazine 22；Propolis 16；O-phthalic
Dibutyl phthalate 24；Sodium alginate 21；Viola japonica 19；Scorpio 13；Centella 18；Marasmius equicrinis Muell 17；Glutinous rehmannia 8.5；And oneself 7.5.
4. the preparation method of the Nano Silver temperature-sensitive hydrogel of the treatment burn and scald as described in claim 1-3 is any, its feature exists
In step is as follows：
（1）Take viola japonica and Marasmius equicrinis Muell to mix, be placed in medicine cookeding pot, slow fire heating stir-fries 7-11 minutes（It is preferred that slow fire heating is turned over
Fry 8-10 minutes）, then drench into the 0.25-0.45 times of sesame oil measured（It is preferred that drenching into the 0.3-0.4 times of sesame oil measured）, continue to stir-fry to
Medicinal material surface slightly has blackspot, take out, with scorpio, centella, glutinous rehmannia and and oneself be together placed in Chinese medicament extracting device, with 4.5-7.5
The ethanol of the raw material dose is solvent, refluxing extraction 1-3 hours again（It is preferred that refluxing extraction 1.5-2.5 hours）, collect alcohol extracting
Liquid, filtering reclaims ethanol, obtains the concentrated liquid of tradition Chinese medicine, standby；
（2）Chitosan, silver nitrate, flamazine and sodium alginate are mixed, quick grinding 2-5 hours is placed in grinder
（Quick grinding 3-4 hours is preferably disposed in grinder）, through ultraviolet light 10-18 hours, chitosan nano silver composite is obtained,
It is standby；
（3）By step（2）Gained chitosan nano silver composite is dissolved into the chitosan nano that mass concentration is 1%-2% with diluted acid
Silver composite solution, step is added into solution（1）Gained the concentrated liquid of tradition Chinese medicine, mixes, obtains mixed solution, standby；
（4）To step（3）Sodium glycero-phosphate, propolis and dibutyl phthalate are added in gained mixed solution, 30- is warming up to
48 DEG C are stirred 18-30 minutes（Stirred 22-26 minutes it is preferred that being warming up to 36-42 DEG C）, then pH adjusted dose adjusts pH to 6.0-
7.0；
（5）Will（4）The solution of gained is put into 35 DEG C of -38 DEG C of thermostat water baths（It is preferably placed into 36 DEG C of -37 DEG C of thermostat water baths
In）, phase in version occurs after 2-6 minutes, immobilising hydrogel is changed into by flowable colloidal solution.
5. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（1）Ethanol
Measure as 5.5-6.5 times of the raw material dose（The amount of preferred alcohol is 6 times of the raw material dose）.
6. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（2）Ultraviolet light
Irradiation 12-16 hours（It is preferred that ultraviolet light 14 hours）.
7. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（3）It is described dilute
Acid is 0.08-0.16mol/L aqueous citric acid solution（It is preferred that the diluted acid be 0.1-0.14mol/L aqueous citric acid solution,
The more preferred diluted acid is 0.12mol/L aqueous citric acid solution）.
8. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（3）It is dissolved into
Mass concentration is 1.3%-1.7% chitosan nano silver complex solution（It is preferred that being dissolved into the chitosan that mass concentration is 1.5%
Nano-ag composite solution）.
9. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（4）PH is adjusted
Agent is disodium hydrogen phosphate.
10. the preparation method of Nano Silver temperature-sensitive hydrogel according to claim 4, it is characterised in that step（4）Adjust pH
To 6.3-6.7（It is preferred that regulation pH to 6.5）.
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| CN102920652A (en) * | 2012-11-26 | 2013-02-13 | 昆明医科大学 | Propolis and chitosan periodontal slow-release thermo-sensitive in-situ gel and preparation method thereof |
| CN105878394A (en) * | 2016-04-11 | 2016-08-24 | 聊城大学 | Nano-silver-containing anti-inflammatory, bactericidal and temperature-sensitive hydrogel and preparation method thereof |
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| CN108126237A (en) * | 2018-01-10 | 2018-06-08 | 中国人民解放军军事科学院军事医学研究院 | A kind of flamazine thermo-sensitive gel and its preparation method and application |
| CN108126237B (en) * | 2018-01-10 | 2022-03-29 | 中国人民解放军军事科学院军事医学研究院 | Silver sulfadiazine temperature-sensitive gel and preparation method and application thereof |
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Application publication date: 20171024 |