CN107281408A - A kind of hypoglycemic traditional Chinese medicine composition and preparation method thereof - Google Patents
A kind of hypoglycemic traditional Chinese medicine composition and preparation method thereof Download PDFInfo
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Abstract
The present invention provides a kind of hypoglycemic traditional Chinese medicine composition and preparation method thereof, is prepared from by radix polygonati officinalis, the root of kudzu vine, agate card, the fruit of Chinese magnoliavine, the stem of noble dendrobium, mulberries and corn stigma.Above-mentioned prescription plays the cooperative effect of each taste bulk drug based on " Yin-nourishing and Qi-supplementing, heat-clearing poison ".All medicine collaborations have polytropism, stage construction, clinical assistant hypoglycemic effect of Mutiple Targets, and have good prevention effect to multiple complications caused by diabetes.Present composition Small side effects, cost be low and preparation process in quality controllability it is strong, suitable for medical large-scale production.
Description
Technical field
The present invention relates to a kind of hypoglycemic traditional Chinese medicine composition and preparation method thereof, belong to pharmaceutical technology field.
Background technology
Diabetes are a kind of chronic lifelong diseases of high illness rate.Counted according to WHO, global diabetic in 2000 is
More than 1.7 hundred million, it is contemplated that the year two thousand thirty will be more than 3.6 hundred million.The people of the existing diabetic of China about more than 4 000 ten thousand, accounts for global glycosuria
The 20% of patient's sum.
Diabetes are due to metabolic syndrome caused by islet function missing or decline, insulin resistance etc., long-term to suffer from
Diabetes can cause the chronic lesion of the multiple systems such as nervous system, cardiovascular system, urinary system, exist in crowd and disable
Rate is high, the death rate is high and the low phenomenon of control rate.Diabetes can be divided into 1 type and 2 types from pathology angle, and type 1 diabetes are pancreas islet
Function is lacked completely, need to be treated with insulin；Diabetes B is supported mainly due to insulin secretion relative deficiency or insulin
Resist and cause impaired glucose tolerance, need to be treated with the medicine for promoting insulin secretion or with insulin sensitizer.Diabetic
Long-term taking medicine control is needed, but at present also there is certain defect in clinical widely used some chemicalses, such as face sulfonylureas, biguanides
Class, alpha-glucosidase inhibitor, insulin sensitizer etc., although blood sugar decreasing effect is fast, but easily causes tolerance, and adverse reaction
Greatly, lesions of liver and kidney is easily caused.
It is generally that a variety of syndromes are mutually mingled with that the diabetes traditional Chinese medical science is called in diabetes, pathogenic process again, clinically multipair sugar
Urine disease carries out TCM Differentiation of Clinical Stages：(1) morbidity early stage is more based on deficiency of both qi and yin, with the passing of time delays, body vital essence increasingly void declines, and can involve
Multi viscera causes the appearance of a variety of ancillary pathologies, the multi-purpose help class Chinese medicine for the treatment of, wherein the effects such as with invigorating the spleen, QI invigorating, enriching yin
Medicine can tonifying five zang organs, strengthening the essence gas, yin-nourishing liquid, adjust negative and positive, function weakness is recovered, while can effectively improve again
Diabetes and its complication；(2) morbidity mid-term based on type of YIN-deficiency and interior-heat, " heat toxin " during diabetes development importantly
Position is paid attention to by doctor, and the application of heat-clearing and detoxifying drug can alleviate diabetes and its complication, and modern pharmacological research and clinic are real
Trample also demonstrate antipyretic and antidotal type Chinese medicine in treating diabetes have good curative effect.
A large amount of practices not only confirm Chinese medicine to diabetes and the obvious curative effects of complication, and are led by its each active component
The action target spot diversity of cause also enjoys the favor of domestic and international pharmaceutical researchers.Therefore, blood glucose can be reduced, reduce concurrently by developing
The Chinese prescription product of disease and edible safety has important clinical meaning and researching value.It is existing to be used to reduce glycemic therapeutic
The Chinese medicine composition of diabetes, although all with a certain degree of blood sugar decreasing effect, but Chinese prescription used is complicated, dosage compared with
Greatly, numerous and diverse, long-term taking liver kidney heavy load is processed, is unfavorable for long-term taking.
The content of the invention
Present invention aims to overcome that there is provided a kind of hypoglycemic traditional Chinese medicine composition and its preparation for above-mentioned the deficiencies in the prior art
Method.The present invention is only made up of 7 herbal medicines, and medicament composing prescription is succinct, consumption is reasonable, with significant blood sugar decreasing effect.Using existing
For extraction process, preparation process is simple, it is easy to industrialized production.
The present invention realized using following technical scheme, a kind of hypoglycemic traditional Chinese medicine composition, in parts by weight, by with
Lower raw material is made：5~50 parts of radix polygonati officinalis, 5~50 parts of the root of kudzu vine, 5~35 parts of agate card, 1~20 part of the fruit of Chinese magnoliavine, 1~10 part of the stem of noble dendrobium, mulberries
1~40 part, 1~40 part of corn stigma.
Preferably, a kind of hypoglycemic traditional Chinese medicine composition of the invention, in parts by weight, is made up of following raw material：Radix polygonati officinalis 15
~35 parts, 15~35 parts of the root of kudzu vine, 10~20 parts of agate card, 5~15 parts of the fruit of Chinese magnoliavine, 3~8 parts of the stem of noble dendrobium, 10~25 parts of mulberries, corn stigma
10~25 parts.
It is highly preferred that a kind of hypoglycemic traditional Chinese medicine composition of the invention, in parts by weight, is made up of following raw material：Radix polygonati officinalis
25 parts, 25 parts of the root of kudzu vine, 15 parts of agate card, 10 parts of the fruit of Chinese magnoliavine, 5 parts of the stem of noble dendrobium, 20 parts of mulberries, 20 parts of corn stigma.
Prescription rule of the present invention follows the organic conception and diagnosis and treatment feature of the traditional Chinese medical science, is with " Yin-nourishing and Qi-supplementing, heat-clearing poison "
It is main, play cooperative effect of each taste bulk drug at hypoglycemic aspect.Radix polygonati officinalis belongs to the product fostered the spirit of nobility and enriched blood, flat mend and moistened in prescription, has
Help the five internal organs, the work(for nourishing qi and blood；The root of kudzu vine tool it is clearing heat and detoxicating, promote the production of body fluid to quench thirst, kidney and spleen invigorating the effects such as, the two effect is distinguished
For monarch drug in a prescription.Agate card and fruit of Chinese magnoliavine enriching yin and nourishing kidney QI invigorating, stem of noble dendrobium replenishing the vital essence and removing heat are moisturized, with enhancing monarch drug in a prescription nourishing yin and supplementing blood, heat-clearing poison
The effect of, therefore be ministerial drug.Mulberries and corn stigma both can be used to strengthen above-mentioned all medicines the effect of, can eliminate or restrict again its it is high it is strong it
Property, it is allowed to medicine for assistant.Above-mentioned all medicine collaborations have polytropism, stage construction, clinical assistant hypoglycemic effect of Mutiple Targets.
Rationally, consumption science is evident in efficacy and stably for inventive formulation, both can be used for directly alleviating insulin function scarce
Hyperglycemic symptoms caused by losing, adjust body oxidativestress damage, mitigate the various complication caused by carbohydrate metabolism disturbance, again may be used
With the effect for the improvement diabetic nephropathy early symptom for playing uniqueness, comprehensively overall treatment is realized.
The preparation method of hypoglycemic traditional Chinese medicine composition of the present invention is, after bulk drug radix polygonati officinalis, mulberries and corn stigma are crushed, plus
Suitable quantity of water immersion is extracted, and obtains the first extract solution；The bulk drug root of kudzu vine, Ma Ka and the fruit of Chinese magnoliavine are crushed after enzymolysis, 80% second
Alcohol immersion is extracted, and obtains the second extract solution；First extract solution is concentrated respectively with second extract solution, returned
Powder is made after receiving solvent, is well mixed with the active compound Dendrobium.
Comprise the concrete steps that：
(1) radix polygonati officinalis, mulberries and corn stigma are dried, the temperature of drying is 40~60 DEG C；Crush, mix；Then plus 5~10
Amount water soaks again, is extracted under the conditions of 80 DEG C~100 DEG C 1~3 time, 30~120min, merges and obtain the first extract solution every time.
(2) root of kudzu vine, Ma Ka and the fruit of Chinese magnoliavine are dried, drying temperature is 40 DEG C~60 DEG C；After crushing, mixing, 3 times of amounts are added
Water and 0.5%~2% cellulase, under the conditions of 35 DEG C~60 DEG C, pH value 4~6, digest 15~60min.Then 10 are added
80% ethanol of~15 times of amounts soaks, and is extracted under the conditions of 50 DEG C~80 DEG C 1~3 time, 30~120min, merges and obtain second every time
Extract solution.
(3) first extract solutions and the second extract solution, because contained active component polarity is different, by the first extract solution and second
Extract solution by being concentrated under reduced pressure, is dried in vacuo into medicinal extract respectively, is ground into powder, is well mixed with Dendrobium (crushing in advance)
.
Preferably, the first extract solution that the step (1) obtains, further with 0.5%~3% chitin (bulking value
Than) flocculated and clarified, 4000rmp centrifugation 10min take supernatant.The flocculation process can effectively remove the tan in decoction
Matter and proteinaceous components.
Preferably, the second extract solution that the step (2) obtains, is further purified with macroporous absorbent resin, discarded
After 20% ethanol eluate, 60%~80% alcohol elution is collected.
Preferably, the step (3) medicinal extract is crushed to 80~120 mesh powders；The Dendrobium granularity is 80
~120 mesh.
Above-mentioned the step of the first extract solution is with the second extract solution order that obtains is not limited.
Chinese medicine composition of the present invention can directly be taken.Further, the present composition can be prepared into various mouths
Oral dosage form, such as agent capsules agent, tablet, pill, powder.Formulation of the present invention is regular dosage form, easily production and storage；To user
Speech, can at any time, quantitatively take, and overcome the condition limitation needed for traditional Chinese medicine is decocted.It should be noted that the agent of said composition
Type is not limited only to above-mentioned formulation, and other formulations that can implement all may be used.
The present invention has the advantages that：
1st, said composition is from Traditional Chinese medical theory, and prescription rule follows the overall and dialectical idea of the traditional Chinese medical science, with " enriching yin
Based on tonifying Qi, heat-clearing poison ", regulation organism physiology balance reaches hypoglycemic effect；In addition, modern pharmacological research is confirmed, the group
Active component in compound can not only correct carbohydrate metabolism disturbance, eliminate symptom, promote the recovery of islet function, improve insulin
Resistance, can also prevent and treat various complication to maintain preferably health and muscle power.
2nd, said composition is derived from pure natural medical composition, and formula is reasonable, consumption science, clinically with collaboration hypoglycemic work(
Effect is especially notable to slight hyperglycaemia hypoglycemic effect；Compared with Western medicine, have no toxic side effect, can long-term taking.
3rd, the present invention is provided preparation method or fully extracted in each herbal medicine with drop blood with water extraction or with enzymolysis alcohol extracting
The component of sugar effect, extracting method is simple and easy to apply, and workable, parameters are controllable, on the premise of drug effect is kept, it is easy to
Industrialized production.
4th, the modern Chinese herbal medicine extraction processes such as " flocculation ", " enzymolysis ", " resin adsorption " are additionally used in preparation process, not only very
The good activity for remaining functional component in natural drug, while higher extraction efficiency is obtained, and to the medication of traditional Chinese medicine
Feature does not have change.
5th, the composition for auxiliary hyperglycemic that the preparation method provided by the present invention is prepared, its biological utilisation
Degree is high, complies with pharmacokinetics rule, and convenient to take, can be very good to meet preparation needs.
Brief description of the drawings
Fig. 1 is the measurement result of the mouse heavy burden swimming time of test example 6.
Note：* compared with blank group, significant difference (P ＜ 0.05)；* is compared with blank group, and difference is extremely significantly (P ＜ 0.01)
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical scheme be clearly and completely described.Unreceipted actual conditions person, builds according to normal condition or manufacturer in embodiment
The condition of view is carried out.Agents useful for same or the unreceipted production firm person of instrument, are the conventional production that can be obtained by commercially available purchase
Product.
A kind of hypoglycemic traditional Chinese medicine composition of embodiment 1., in parts by weight, is made up of following raw material：25 parts of radix polygonati officinalis, Pueraria lobota
25 parts of root, 15 parts of agate card, 10 parts of the fruit of Chinese magnoliavine, 5 parts of the stem of noble dendrobium, 20 parts of mulberries, 20 parts of corn stigma.
Preparation method is：Radix polygonati officinalis, 40 DEG C of dries pulverizings of mulberries and corn stigma, plus 10 times of 80 DEG C of amount water are extracted 3 times, merged
Extract solution, adds the flocculation of 0.5% (w/v) chitin, obtains the first extract solution.By 40 DEG C of the root of kudzu vine, Ma Ka and the fruit of Chinese magnoliavine
Dries pulverizing, adds 3 times of amount water and 1.5% cellulase (w/v), under the conditions of 35 DEG C, pH value 5, enzymolysis
30min.After enzymolysis, 15 times of 80% ethanol of amount are added, 50 DEG C are extracted 3 times, each 60min merges extract solution.Extract solution is through D101
Purification with macroreticular resin, collects 60%~80% ethanol eluate and obtains the second extract solution；First extract solution is carried with second
Liquid is taken to be concentrated under reduced pressure respectively, crushed after being dried is mixed into 80 mesh powders with active compound Dendrobium (being ground into 80 mesh in advance)
It is even, obtain composition.
A kind of hypoglycemic traditional Chinese medicine composition of embodiment 2., in parts by weight, is made up of following raw material：15 parts of radix polygonati officinalis, Pueraria lobota
15 parts of root, 10 parts of agate card, 5 parts of the fruit of Chinese magnoliavine, 5 parts of the stem of noble dendrobium, 10 parts of mulberries, and 10 parts of corn stigma.
Preparation method is：Radix polygonati officinalis, 50 DEG C of dries pulverizings of mulberries and corn stigma, plus 5 times of 90 DEG C of amount water are extracted 2 times, merged
Extract solution, adds the flocculation of 1% (w/v) chitin, obtains the first extract solution.By the root of kudzu vine, 50 DEG C of bakings of Ma Ka and the fruit of Chinese magnoliavine
Dry grinding, adds 3 times of amount water and 1% cellulase (w/v), under the conditions of 40 DEG C, pH value 5.5, digests 40min.
After enzymolysis, add 10 times of 60 DEG C of 80% ethanol of amount and extract 3 times, each 70min merges extract solution.Extract solution is inhaled through D101 macropores
Attached purifying resin, collects 60%~80% ethanol eluate and obtains the second extract solution；By the first extract solution and the second extract solution point
It is not concentrated under reduced pressure, crushed after being dried is well mixed with active compound Dendrobium (being ground into 90 mesh in advance), obtained into 100 mesh powders
To composition.
A kind of hypoglycemic traditional Chinese medicine composition of embodiment 3., in parts by weight, is made up of following raw material：35 parts of radix polygonati officinalis, Pueraria lobota
35 parts of root, 20 parts of agate card, 15 parts of the fruit of Chinese magnoliavine, 8 parts of the stem of noble dendrobium, 25 parts of mulberries, 25 parts of corn stigma.
Preparation method is：Radix polygonati officinalis, 60 DEG C of dries pulverizings of mulberries and corn stigma, plus 8 times of 100 DEG C of amount water are extracted 2 times, merged
Extract solution, adds the flocculation of 3% (w/v) chitin, obtains the first extract solution.By the root of kudzu vine, 50 DEG C of bakings of Ma Ka and the fruit of Chinese magnoliavine
Dry grinding, adds 3 times of amount water and 2% cellulase, under the conditions of 50 DEG C, pH value 6, digests 60min.After enzymolysis, 13 are added
80 DEG C of 80% ethanol of amount is extracted 2 times again, and each 90min merges extract solution.Extract solution is received through D101 purification with macroreticular resin
60%~80% ethanol eluate of collection obtains the second extract solution；First extract solution is concentrated under reduced pressure respectively with the second extract solution, done
100 mesh powders are ground into after dry, is well mixed with active compound Dendrobium (being ground into 100 mesh in advance), obtains composition.
A kind of hypoglycemic traditional Chinese medicine composition of embodiment 4., in parts by weight, is made up of following raw material：50 parts of radix polygonati officinalis, Pueraria lobota
50 parts of root, 35 parts of agate card, 20 parts of the fruit of Chinese magnoliavine, 10 parts of the stem of noble dendrobium, 40 parts of mulberries, 40 parts of corn stigma.
Preparation method be the same as Example 1.
A kind of hypoglycemic traditional Chinese medicine composition of embodiment 5., in parts by weight, is made up of following raw material：5 parts of radix polygonati officinalis, the root of kudzu vine
5 parts, 5 parts of agate card, 1 part of the fruit of Chinese magnoliavine, 1 part of the stem of noble dendrobium, 1 part of mulberries, 1 part of corn stigma.
Preparation method be the same as Example 1.
A kind of hypoglycemic capsule of embodiment 6., in parts by weight, is matched as follows：
Conventionally, capsule is made in component.
A kind of hypoglycemic tablet of embodiment 7., in parts by weight, is matched as follows：
Conventionally, tablet is made in said components.
A kind of hypoglycemic pill (water-bindered pill) of embodiment 8., in parts by weight, matches as follows：
Conventionally, pill is made in said components.
A kind of hypoglycemic powder of embodiment 9., in parts by weight, is matched as follows：
100 parts of composition made from embodiment 4
15 parts of calcium lactate
Conventionally, powder is made in said components.
Influence of the present composition of test example 1. to blood glucose in diabetic mice content
1st, operating method and step
After normal KM mouse fasting 12h, Streptozotocin 150mg/kg modelings are injected.Choose the successful mouse 50 of modeling
Only, 5 groups are randomly divided into：The basic, normal, high dosage of composition prepared by model group, melbine group (40mg/kg), and embodiment 1
Group, dosage is respectively 100,200,400mg/kg, separately set Normal group, be administered once daily, ad lib, drinking-water.Experiment week
4 weeks phases, 1,2,3,4 weekend tail vein bloods are being tested, fasting blood sugar is surveyed.
2nd, result and analysis
Influence (n=10, X ± SD) of the composition of table 1 to blood glucose in diabetic mice content
Note：* compared with model group, significant difference (P ＜ 0.05)；* is compared with model group, and difference is extremely significantly (P ＜ 0.01)
As shown in Table 1, the blood-sugar content of diabetic mice was raised apparently higher than the blood-sugar content of normal group mouse by 4 weeks
Support, the blood-sugar content of model group diabetic mice is nearly 5 times of normal mouse；By the treatment of various dose Chinese medicine composition
Afterwards, there is reduction trend, especially high dose group diabetic mice and given in the middle and high dosage group blood glucose in diabetic mice content of composition
Blood-sugar content of the medicine after 3 weeks is reduced significantly (P ＜ 0.05) compared with the diabetic mice of untreated, as a result illustrates said composition
With certain blood sugar decreasing effect, and it is presented as dose dependent.
Influence of the present composition of test example 2. to diabetic mice serum insulin and hepatic glycogen content
1st, operating procedure
After being administered 4 weeks according to the step in test example 1, mouse femoral artery is taken a blood sample, serum insulin level is determined.Dislocation
Put to death, take liver to be placed in ice bath at once, and cleaned with physiological saline, filter paper wipes dry, weighs appropriate, and matter is made with ice physiological saline
Amount concentration is 100mg/0.5mL liver homogenates, is determined for hepatic glycogen content.
2nd, result and analysis
Influence (n=10, X ± SD) of the composition of table 2 to diabetic mice serum insulin and hepatic glycogen content
Note：* compared with model group, significant difference (P ＜ 0.05)；* is compared with model group, and difference is extremely significantly (P ＜ 0.01)
Compared with diabetic mice serum insulin content, composition administration group mice serum insulin content rises
Trend, the content of 400mg/kg dosage group mice serum insulin is 1.8 times of diabetic mice serum insulin content, is presented
Significant difference.With the increase of insulin content, also there is increase trend in mouse hepatic glycogen content, and particularly composition administration is high
Dosage group, the content of hepatic glycogen is 3.9 times of model group, illustrates that it can promote the synthesis of hepatic glycogen.
Influence of the present composition of test example 3. to diabetes rat sugar tolerance
1st, operating method and step
Normal SD rats fasting be can't help after water 12h, with 0.1M Streptozotocins：0.1M citrate buffers (pH4.5) are pressed
Diabetes animal model is set up in the intraperitoneal injection of 50mg/Kg dosage.Rat fasting blood-glucose is determined with blood glucose meter after 3 days, blood glucose value is big
Tested in 10mmol/L rat for sugar tolerance.
Diabetes rat fasting be can't help after water 12h, be randomly divided into 5 groups, every group 6：Model group, melbine group
The basic, normal, high dosage group of composition (100mg/Kg, 200mg/Kg, 400mg/Kg) prepared by (100mg/kg), embodiment 1, separately sets
Disposable gavage glucose solution (2g/kg) after Normal group, perfusion 30min, gives glucose 30,60 and 120min is determined
Blood glucose value, investigates influence of the various dose component to blood glucose rise after diabetes rat glucose load, the results are shown in Table 3.
2nd, result and analysis
Influence (n=6, X ± SD) of the composition of table 3 to diabetes rat sugar tolerance
Note：* compared with model group, significant difference (P ＜ 0.05)；* is compared with model group, and difference is extremely significantly (P ＜ 0.01)
As shown in Table 3, the middle and high dosage group rat blood sugar TG-AUC (AUC) of composition is below model group, and high agent
Amount group shows significant difference (P ＜ 0.05).Show that said composition has reduction blood glucose in diabetic rats TG-AUC
Effect, can improve the impaired glucose tolerance condition of diabetes rat.
To sum up, diabetes are due to metabolic syndrome caused by islet function missing or decline, insulin resistance etc..Pancreas
Island element can stimulate the synthesis of hepatic glycogen and suppress liver glycogen phosphorylase, so that promote the deposition of hepatic glycogen, therefore hepatic glycogen
Content very close relationship is also have with pancreas islet.Test example 1-3 shows, the hypoglycemic mechanism of action of said composition may be with
It is relevant that it can repair impaired pancreas beta cell, by a large amount of excreting insulins of stimulating pancreas beta cell, so as to play reduction
Blood glucose, the effect for improving sugar tolerance and promoting hepatic glycogen to synthesize.
The hypoglycemic traditional Chinese medicine composition that the present invention is provided is evident in efficacy, before its related assistant hypoglycemia healthcare food exploitation
Scape is good.
Influence of the present composition of test example 4. to SOD and MDA in diabetes rat serum
1st, operating method and step
According to the method modeling in test example 3, diabetes rat is randomly divided into 5 groups, every group 10, including：Model group,
The basic, normal, high dosage group of composition prepared by melbine group (100mg/kg) and embodiment 1 (100mg/Kg, 200mg/Kg,
400mg/Kg), Normal group is separately set, continuous medicine-filling is after 60 days, and eye socket takes blood, determines the SOD vigor in every rat blood serum
With MDA content.
2nd, result and analysis
Influence (n=10, X ± SD) of the composition of table 4 to SOD and MDA in diabetes rat serum
Note：* compared with model group, significant difference (P ＜ 0.05)；* is compared with model group, and difference is extremely significantly (P ＜ 0.01)
From table 4, it can be seen that the activity of superoxide dismutase (SOD) is decreased obviously in diabetic controls group serum, and
The content of metabolism of lipid peroxide product malonic acid (MDA) substantially then increases.Said composition administration group is to above-mentioned SOD and MDA
Variation tendency has improvement result；Especially middle and high dosage group effect is more notable.
Research shows that oxygen radical plays very important effect in the generation, development of chronic complicating diseases of diabetes.Fat
Matter peroxidation also plays a role in the pathogenesis of chronic complicating diseases of diabetes.Diabetic's interior free yl is big
Amount increases, and itself removes the ability wretched insufficiency of free radical, therefore the free radical duration of high concentration is longer, diabetes
Complication rate is just higher more serious.Provided by the present invention for assistant hypoglycemic composition to Serum of Diabetes Rats
In SOD vigor and MDA contents tool improve significantly, illustrate that it has functions that oxidation and removing free radicals in vivo,
Available for the treatment for alleviating diabetic's chronic complicating diseases.
The agate card of test example 5. is cooperateed with other drugs to blood glucose and the contrast of kidney regulatory function
1st, operating method and step
Chain arteries and veins assistant rhzomorph is dissolved in 0.1M citric acids acid buffer (pH4.5), is built by 45mg/Kg dosage intraperitoneal injection SD rats
Vertical diabetes animal model, and add hello high glucose and high fat feed.After A group modelings the embodiment of the present invention 1 is gavaged by 300mg/kg dosage
The composition of preparation；Gavaged after B group modelings and dosage is waited made from same procedure without agate card composition；Physiology is gavaged after C group modelings
Salt solution；Separately set normal control D groups；It is administered once a day, empty stomach 12h after testing 4 weeks, tail vein blood detection fasting blood-glucose and sugar
Base hemoglobin changes, and collects urine detection urine micro protein level.
2nd, result and analysis
Influence (n=10, X ± SD) of the table 5 to rat blood sugar, glycosylated hemoglobin and microdose urine protein
Note：* compared with model group, significant difference (P ＜ 0.05)；* is compared with model group, and difference is extremely significantly (P ＜ 0.01)
As can be seen from Table 5, the composition of card containing agate is glycosylated with having significant hypoglycemic without agate card composition and improving
Effect (the P of hemoglobin<0.05).The group of card containing agate compares without agate card group effect in terms of rat microdose urine protein excretion rate is reduced
Fruit is more notable.Two groups can reduce the blood sugar level of early diabetes patient, and agate card group improves early diabetes patient's kidney
The effect of disease symptoms is more excellent.
As can be seen from the above results, add with other 6 taste Chinese medicines to have after agate card and act synergistically, strengthen its hypoglycemic
Effect, and with significantly improving the unexpected effect of nephrosis function.Speculate due to the active component maca in maca
Alkene, macamide etc. can directly act on pituitary gland, and pituitary gland improves the physiology machine of renal metabolism by secreting hormone
Renal function, can be improved, and then adjust the operating of the major organs of human body.
The present composition of test example 6. improves fatigue function
1st, operating method and step
60 body weight are randomly divided into 6 groups, respectively blank control group, reality for (20 ± 5) g ICR pure lines male mices
Apply the basic, normal, high dosage group of composition (100mg/Kg, 200mg/Kg, 400mg/Kg) of the preparation of example 1, every group of daily gastric infusion
Once, American Ginseng is as positive drug, and dosage is 1.2g/Kg, and blank control group is to distilled water, successive administration 15d.Last
Fasting 2h after administration, every negative sheet lead from body quality 5% of mouse tail, is placed in swimming groove and swims, depth of water 40cm, water temperature
(25 ± 1) DEG C, record exhausts the time to power from swim.Mouse power exhausts criterion：All enter water with mouse head to continue
8s can not swim out of the water surface to judge terminal.
2nd, result and analysis
Experimental result is as shown in Figure 1.The raising of exercise tolerance is that anti-fatigue ability strengthens most strong macro manifestations, swimming
The length of time can reflect the degree of animal movement fatigue.As shown in Figure 1, the basic, normal, high dosage group swimming time of composition is equal
It is longer than blank control group, and high dose group is suitable with American Ginseng effect, with statistical significance.Illustrate that said composition has obvious
Anti-fatigue effect, have broad application prospects in related health food development.
Clinical practice finds that tiredness is also common a kind of main complaint in diabetic.Diabetes are sick in itself
Journey length, complication are more, can not cure, and its pathological characteristic and caused physiological reaction and psychological problems etc. can all cause patient to go out
Existing fatigue symptom.Long-term fatigue state can influence physical and mental health, work and study and the daily life of individual, cause quality of life
Decline, control not only bad for the state of an illness and the self-management behavior of patient can be hindered.
In summary, provided by the present invention for assistant hypoglycemic composition and preparation method thereof and utilization, it is reasonable that it is formulated,
Consumption science, it is evident in efficacy and stably, it both can be used for directly alleviating hyperglycemic symptoms caused by insulin function missing, regulation
Body oxidativestress damage, mitigates the various complication caused by carbohydrate metabolism disturbance, and the improvement diabetes of uniqueness can be played again
The effect of nephrosis early symptom, realizes comprehensively overall treatment.The preparation method is simple to operate, and parameters are controllable, beneficial to big
Large-scale production, with great social effect and economic value.
Embodiments described above is a part of embodiment of the invention, rather than whole embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiment in the present invention, what those of ordinary skill in the art were obtained under the premise of creative work is not made
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of hypoglycemic traditional Chinese medicine composition, it is characterized in that, in parts by weight, it is made up of following raw material：5~50 parts of radix polygonati officinalis,
5~50 parts of the root of kudzu vine, 5~35 parts of agate card, 1~20 part of the fruit of Chinese magnoliavine, 1~10 part of the stem of noble dendrobium, 1~40 part of mulberries, 1~40 part of corn stigma.
2. hypoglycemic traditional Chinese medicine composition as claimed in claim 1, it is characterized in that, in parts by weight, it is made up of following raw material：
15~35 parts of radix polygonati officinalis, 15~35 parts of the root of kudzu vine, 10~20 parts of agate card, 5~15 parts of the fruit of Chinese magnoliavine, 3~8 parts of the stem of noble dendrobium, 10~25 parts of mulberries,
10~25 parts of corn stigma.
3. hypoglycemic traditional Chinese medicine composition as claimed in claim 1 or 2, it is characterized in that, in parts by weight, by following raw material system
Into：25 parts of radix polygonati officinalis, 25 parts of the root of kudzu vine, 15 parts of agate card, 10 parts of the fruit of Chinese magnoliavine, 5 parts of the stem of noble dendrobium, 20 parts of mulberries, 20 parts of corn stigma.
4. hypoglycemic traditional Chinese medicine composition method described in claim 1 is prepared, it is characterized in that, radix polygonati officinalis, mulberries and corn stigma are crushed
Afterwards, plus suitable quantity of water immersion extracted, obtain the first extract solution；The root of kudzu vine, Ma Ka and the fruit of Chinese magnoliavine are crushed after enzymolysis, 80% ethanol
Immersion is extracted, and obtains the second extract solution；First extract solution is concentrated respectively with the second extract solution, made after recycling design
Into powder, it is well mixed with Dendrobium.
5. preparation method as claimed in claim 4, it is characterized in that, comprise the following steps that：
(1) radix polygonati officinalis, mulberries and corn stigma are dried, the temperature of drying is 40~60 DEG C；Crush, mix；Then plus 5~10 times of amounts
Water soaks, and is extracted under the conditions of 80 DEG C~100 DEG C 1~3 time, 30~120min, merges and obtain the first extract solution every time；
(2) root of kudzu vine, Ma Ka and the fruit of Chinese magnoliavine are dried, drying temperature is 40 DEG C~60 DEG C；Crush, mixing after, add 3 times amount water and
0.5%~2% cellulase, under the conditions of 35 DEG C~60 DEG C, pH value 4~6, digests 15~60min；Then 10~15 are added
80% ethanol of amount soaks again, is extracted under the conditions of 50 DEG C~80 DEG C 1~3 time, 30~120min, merges and obtain the second extraction every time
Liquid；
(3) by the first extract solution with the second extract solution respectively by being concentrated under reduced pressure, be dried in vacuo into medicinal extract, be ground into powder, with
The Dendrobium crushed in advance is well mixed.
6. preparation method as claimed in claim 5, it is characterized in that, the first extract solution that the step (1) obtains further is used
0.5%~3% chitin is flocculated and clarified, 4000rmp centrifugation 10min, takes supernatant.
7. preparation method as claimed in claim 5, it is characterized in that, the second extract solution that the step (2) obtains further is used
Macroporous absorbent resin is purified, and is discarded after 20% ethanol eluate, collects 60%~80% alcohol elution.
8. preparation method as claimed in claim 5, it is characterized in that, medicinal extract is crushed to 80~120 mesh described in the step (3)
Powder；The Dendrobium granularity is 80~120 mesh.
9. preparation method as claimed in claim 5, it is characterized in that, the preparation order of first extract solution and the second extract solution
Do not limit.
10. the medicine containing hypoglycemic traditional Chinese medicine composition described in claim 1, it is characterized in that, the medicine be capsule, tablet,
Pill, powder.
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Application publication date: 20171024 |