CN107280771B - Life body experimental device for simulating ocean long-distance navigation state - Google Patents
Life body experimental device for simulating ocean long-distance navigation state Download PDFInfo
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- CN107280771B CN107280771B CN201610196887.5A CN201610196887A CN107280771B CN 107280771 B CN107280771 B CN 107280771B CN 201610196887 A CN201610196887 A CN 201610196887A CN 107280771 B CN107280771 B CN 107280771B
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B2503/00—Evaluating a particular growth phase or type of persons or animals
- A61B2503/40—Animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a life body experimental device for simulating a marine long-voyage state, which is provided with a test box, wherein a plurality of floating platforms are arranged in the test box, a wave making pump is arranged in the test box, water is filled in the test box, the floating platforms are in a floating state, and small animals are placed on the floating platforms in advance; and starting the wave making pump to make waves, wherein the floating platform presents the bumpy state of the floating objects on the water, and the marine long-distance simulation test is carried out on the floating platform by small animals. The project provides a device foundation for modeling of long-range fatigue, long-range motion sickness and other cognition and emotion change related diseases by unique collocation of simple factors, and can flexibly control modeling time and intensity, thereby completing modeling of different diseases and different syndromes.
Description
Technical Field
The invention relates to a life body experimental device for simulating a marine long-voyage state, in particular to a pontoon platform used in experiments.
Background
The ocean occupies two thirds of the earth, and along with the deepening of ocean exploration and the development of navigation military, navigation related health problems gradually become research hotspots. IMO (international maritime organization) surveys show that over 80% of marine accidents are caused by human factors. Solves the long-distance aviation health problem, not only is the foundation for the development of ocean industry, but also is the health guarantee for improving the navy combat force of China.
Due to the special environment, work and rest, society and other factors, the long-term navigation can cause a series of bad changes of physical and mental states of the human body, wherein the occurrence rate of long-term navigation fatigue is highest. The animal model is the basis for carrying out the biological mechanism of the diseases and the research of medicines, but at present, no animal model aiming at long-range fatigue exists at home and abroad, and the difficulty of operation is greatly improved by taking the animal on a long-range ship. The establishment of a rat model highly simulating a long-range environment has important significance for the mechanism research and drug research and development of long-range related diseases such as long-range fatigue, motion sickness and the like.
In the long-term research of the long-term endurance, the biological mechanism of the long-term endurance is still in an exploration stage due to the complex influencing factors and multidimensional psychological and physiological manifestations, and great difficulty is brought to the mechanism research and clinical control of the long-term endurance.
Disclosure of Invention
Aiming at the problems existing in the prior art, the invention aims to provide a life body experimental device for simulating a long-voyage state of ocean, which is a floating platform device for simulating a long-voyage environment to model rats, and is characterized in that long-voyage multidimensional interference factors such as a single water surface environment, floating sense, repeated operation and the like are systematically constructed and matched to simulate the long-voyage environment, and all the factors are comprehensively combined and designed together through the system of the device to form a unique long-voyage fatigue rat model innovation device.
The device provides a convenient model guarantee for the research of long-range fatigue, long-range motion sickness, and even long-range related diseases in other aspects such as cognitive function, emotion and the like.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the life body experimental device for simulating the ocean long-voyage state is provided with a test box, wherein a plurality of floating platforms are arranged in the test box, a wave making pump is arranged in the test box, water is filled in the test box, the floating platforms are in a floating state, and small animals are placed on the floating platforms in advance; and starting the wave making pump to make waves, wherein the floating platform presents the bumpy state of the floating objects on the water, and the marine long-distance simulation test is carried out on the floating platform by small animals.
Further, the floating platform is hollow and cylindrical or truncated cone-shaped, and the floating state in the test box is adjusted by filling water into the inner cavity in advance.
Further, a plurality of fixing frames are arranged in the test box, the floating platforms are all placed in the fixing frames, and the fixing frames prevent the floating platforms from tipping due to jolting; the bottom end of the floating platform is provided with a pull rope which is tied on the bottom of the test box.
Further, the fixing frame is in a frame shape, a plurality of horizontal annular frames and supporting legs are arranged, and the bottom ends of the supporting legs are fixed on the bottom of the test box.
Further, the upper end surface of the floating platform is a rough surface or is provided with anti-skid lines; the floating platform is provided with 12, make unrestrained pump and be provided with 1, the floating platform is around make unrestrained pump setting.
Further, the test box is provided with a water inlet valve and a water outlet valve, and the outlet end of the water inlet valve is provided with a filter screen.
Further, the test box is provided with a box cover which is net-shaped, and a food and drinking water bottle is hung on the box cover for small animals to eat food and water; and the case cover is covered with shading cloth.
Further, the test box is provided with a box cover which is made of transparent materials, and a plurality of food grooves are formed in the box cover, and food and drinking water bottles are inserted into the food grooves to enable small animals to eat food and water; and the case cover is covered with shading cloth.
Furthermore, the test box and the fixing frame are both made of stainless steel, and the fixing frame is fixed with the bottom of the test box by adopting a welding or screw connection structure.
Further, the wave making pump power is adjustable; the wave making pump is provided with a plurality of wave making pumps.
The life body experimental device set by adopting the structure under the simulated ocean long-voyage state has the following advantages:
The project provides a device foundation for modeling of long-range fatigue, long-range motion sickness and other cognition and emotion change related diseases by unique collocation of simple factors, and can flexibly control modeling time and intensity, thereby completing modeling of different diseases and different syndromes.
The establishment of animal models has great significance for mechanism research and drug effect observation. The development and popularization of the project provide a model foundation for the mechanism research of the long-range fatigue, are more beneficial to developing the research of treating the long-range fatigue by using the traditional Chinese medicine, fully exert the medical advantages of the traditional Chinese medicine, and finally provide health guarantee for the improvement of the navy fight force of China and the development of ocean industry. The device provides a model foundation for the subject research. In addition, the floating small platform can flexibly control conditions and modeling intensity, can be widely applied to the mechanism and medicine development research of other diseases such as long-voyage related diseases, cognitive function decline, mood disorder and the like, and has profound significance.
Drawings
FIG. 1 is a front view of the present invention;
FIG. 2 is a top view of an experimental box used in the present invention;
FIG. 3 is a top plan view of a cover for use with the present invention;
FIG. 4 is a schematic diagram of a trough structure provided on a cover;
FIG. 5 is a front view of a floating platform for use with the present invention;
FIG. 6 is a front view of a floating platform for use with the present invention;
FIG. 7 is a combined state diagram of a mount and floating platform used in the present invention;
FIG. 8 is a perspective view of a mount for use with the present invention;
FIG. 9 is a top plan view of a cover for use with the present invention;
fig. 10 is a use state diagram of the present invention.
In the figure: 1. a case cover; 1-1, a trough; 2. an experiment box; 3. a water outlet valve; 4. a wave making pump; 5. a floating platform; 5-1, a cylinder; 5-2, a water filling port; 5-3, pulling ring; 6. a water inlet valve; 7. a pull rope; 8. and a fixing frame.
Detailed Description
In order to further describe the technical means and effects adopted by the present invention for achieving the predetermined technical purpose, the following detailed description of the structure, features and effects of the present invention is given with reference to the accompanying drawings and preferred embodiments.
Example 1
Referring to fig. 1,2 and 3, an embodiment of the present invention is shown, in which a living body experimental apparatus for simulating a marine long-voyage state is provided, and a test box 2 is provided, a plurality of floating platforms 5 are provided in the test box 2, a wave-making pump 4 is provided to fill water into the test box 2, the floating platforms 5 are in a floating state, and small animals are placed on the floating platforms 5 in advance; the wave making pump 4 is started to make waves, the floating platform 5 presents the bumpy state of the floating objects (mainly water ships), and the marine long-distance simulation test is carried out on the floating platform 5 by small animals.
The test chamber 2 is a circular water tank structure with an opening at the upper end. Therefore, the water wave around each floating platform and the rebound water wave after the water wave hits the wall are equal, and the floating platform cannot generate experimental errors due to different waves.
The animals to be tested are mainly mice, such as rats and mice, which can stay or move on top of the floating platform 5.
The floating platform 5 is hollow cylindrical or frustoconical and adjusts the floating conditions in the test chamber by pre-filling the cavity with water, for example at different depths in the water. The floating platform 5 shown in fig. 5 is cylindrical, the floating platform 5 shown in fig. 6 is in a truncated cone shape, the top end of the floating platform is provided with a water injection port 5-2, and a sealing cover is arranged at the water injection port 5-2, so that the floating platform can adopt a threaded sealing structure and cannot be opened by small animals.
A plurality of fixing frames 8 are arranged in the test box 2, the floating platforms 5 are all placed in the fixing frames 8, and the fixing frames 8 prevent the floating platforms 5 from tipping due to jolting; the bottom end of the floating platform 5 is provided with a pull rope which is tied on the bottom of the test box 2.
As shown in fig. 5 and 6, the bottom end of the cylinder 5-1 is provided with a pull ring 5-3, the bottom of the test box 2 is also provided with a pull ring, the pull ring and the bottom are tied by a pull rope 7, the floating platform 5 is limited by the fixed frame 8, the floating platform 5 is also limited by the pull rope 7, and the pull rope 7 is mainly used for preventing the floating platform 5 from being interfered by small animals to have an abnormal state, for example, the floating platform is interfered by the small animals to be blocked by the fixed frame 8 so as not to jolt any more.
As shown in fig. 7 and 8, the fixing frame 8 is frame-shaped, and is provided with 2 horizontal annular frames and 3 supporting legs, and the bottom ends of the supporting legs are fixed on the bottom of the test box 2.
The upper end surface of the floating platform 5 is a rough surface or is provided with anti-skid lines, so that small animals can conveniently stay or move at the top end of the floating platform 5.
The number of floating platforms 5 is 12, the number of wave-making pumps 4 is 1, and the floating platforms 5 are arranged around the wave-making pumps 4. The experimental device is arranged for increasing experimental objects, so that more experimental data are obtained, and experimental accuracy is improved.
The test chamber 2 is provided with a water inlet valve 6 and a water outlet valve 4, and the outlet end of the water inlet valve 6 is provided with a filter screen. The filter screen is used to prevent debris from entering the test chamber 2.
The animal manure can be discharged out of the test chamber 2 along with water through the water outlet valve 3.
As shown in fig. 3, the test box 2 is provided with a box cover 1, the box cover 1 is made of transparent material, the box cover 1 is provided with a plurality of food grooves 1-1, and food and drinking water bottles are inserted into the food grooves 1-1 for small animals to eat food and water; the cover 1 is covered with a light shielding cloth (not shown). The trough 1-1 may be located in a position between two floating platforms 5.
The case cover 1 may be made of organic glass (acryl). The light shielding cloth was covered to create a closed and boring environment for rats. When the condition in the box is required to be observed, the shading cloth is lifted for observation.
As shown in fig. 4, the bottom end of the trough 1-1 is designed to be inclined, the side wall is provided with an outlet, and the food strips for feeding rats extend out of the outlet for the rats to eat. The water bottle can be inverted to slowly drip water or be provided with a conduit to allow the rat to absorb water from the conduit.
The test box 2 and the fixing frame 8 are made of stainless steel, and the fixing frame 8 is fixed with the bottom of the test box 2 by adopting a welding or screw connection structure.
The power of the wave making pump 4 can be adjusted. This is arranged to obtain different levels of waves.
A. The reference dimensions of the test chamber are:
the diameter of the bottom surface of the circular water tank is 100cm, and the height is about 35 cm. Water is injected into the water tank, the water surface height is 12.5cm, and the water temperature is maintained at 22.0+/-2.0 ℃.
The requirements are: 1. the height of the water tank should be such that the rat stands on the platform, and the rat just touches the tank cover in the standing state, so that on one hand, the rat can be ensured to eat food and water smoothly, and on the other hand, a single narrow space state of the rat is provided
2. Each water tank is provided with a cover made of organic glass material, so that mice are prevented from jumping out of the water tank. The cover is required to be firm and durable, and mice can not bite, and the cover is matched with the water tank.
3. Each water tank is provided with a water valve, so that water can be conveniently discharged.
B. the reference dimensions of the floating platform are:
The platform is cylindrical, the diameter of the upper bottom and the lower bottom is 8.5cm, and the height is 11cm.
The requirements are: 1. the material is firm and not bitten by mice but not thick, and the mass is 80 g-100 g
2. The sealing performance is good, but the screw cover which can be opened is arranged, so that water can be conveniently added into the screw cover.
3. The upper surface of the cylindrical pontoon is rough and has shallow grooves, the lower surface is provided with small circular rings, and the corresponding box bottom is also provided with circular rings, so that the water tank and the pontoon are conveniently connected by ropes.
C. The reference dimensions of the fixing frame are as follows:
Is a triangle frame, two circular iron rings are fixed on the triangle frame, the caliber of the upper ring is 9.5cm, and the caliber of the lower ring is 10cm.
The requirements are: 1. the material is even, firm, and does not rust, firmly connects on the water tank bottom surface.
2. The distance between the upper and lower iron rings is about 3.5-4 cm, and cannot be too large or too small.
3. The height of the iron frame is determined according to the depth of water required by the wave making device, and the height is 3cm higher than the depth of water required by the wave making device.
4. The interval between the fixing frames is 12cm.
The application method of the invention is introduced as follows:
As shown in fig. 10, during experimental operation, rats are placed on a floating platform, and due to the nature of water fear, the rats can be prevented from entering water and keeping the muscles stressed to stand on the platform, when a water wave generator makes the water surface shake, the rats on the floating platform can float along with the water surface and shake involuntarily, and due to the fact that each platform is provided with a bracket for controlling the floating range and amplitude, the floating degree cannot be too large, so that the rats fall into the water surface due to rollover. The experimenter can control the time length and the nodes of the floating small platform, so that long-endurance fatigue molding is performed. The control tap is arranged at the lower end of the water tank, so that water can be changed conveniently every day. The water tank is provided with a net cover with uniform size, a food frame and a water bottle are hung, and rats can eat and feed water freely on the platform.
The diameter of the bottom surface of the pontoon platform is 8.5cm, and by adding shaking factors and combining literature reports, a rat can not get a rest and sleep when standing on the pontoon. According to the buoyancy principle, g=ρ×g×v (G is the buoyancy force exerted by the object, G is the gravitational acceleration, ρ is the water density, and V is the volume of the buoy immersed in the water), the buoyancy force exerted by the buoy in the water is about 620G, the gravity of the self is subtracted, and the maximum bearable weight of the buoy in the water is 450G, so that the buoy can shake freely. Because the pontoon platform is hollow structure, it can be inside water injection and guarantee that every time the rat stands, its distance from the surface of water is certain, can reduce the experimental error like this, guarantees its accuracy. The surrounding brackets are built, so that the shaking of the pontoon can be controlled within a certain range, and the rats can not be overturned into the water due to the fact that the pontoon can not be overturned. In a plurality of simulation experiments, relevant exact proper bracket dimensions (as described above) are obtained, so that the normal and effective operation of the pontoon platform can be ensured.
In addition, when the water wave generator works, noise such as water wave sound can be generated to a certain extent, the characteristics of shaking, noise and single environment of the long-distance ship are more attached, and the time node and the length of the floating platform are flexibly controlled by experimenters to regulate and control modeling influencing factors of work and rest disorder.
The model is formed for rats in an omnibearing and multilevel mode, the superposition of multiple influencing factors is completed from the construction efficiency of the model, and the model has the advantages of convenience in operation and flexibility in control.
Example 2
Unlike in embodiment 1, in this embodiment, a plurality of wave-making pumps (not shown) are provided, and the plurality of wave-making pumps are provided at the center, the periphery, and the like of the test chamber.
Providing a plurality of wave-making pumps enables more complex waves to be made, closer to the actual sea conditions.
The case cover 1 can also be net-shaped, and the case cover 1 is hung with a food and drinking water bottle for the small animals to eat food and water. As shown in fig. 9, the case cover 1 may be configured by attaching a frame of a profile to a steel wire mesh, and the case cover 1 is also covered with a light shielding cloth (not shown).
The above description is only for the purpose of illustrating the invention, and it should be understood that the invention is not limited to the above embodiments, but various modifications consistent with the idea of the invention are within the scope of the invention.
Claims (7)
1. The life body experimental device for simulating the ocean long-voyage state is characterized by comprising a test box, wherein a plurality of floating platforms are arranged in the test box, a wave making pump is arranged in the test box, water is filled in the test box, the floating platforms are in a floating state, and small animals are placed on the floating platforms in advance; the wave making pump is started to make waves, the floating platform is in a bumpy state of floating objects on the water, and marine long-range simulation tests are carried out on the floating platform by small animals, wherein a plurality of fixing frames are arranged in the test box, the floating platform is placed in the fixing frames, and the fixing frames prevent the floating platform from tipping due to bumping; a pull rope is arranged at the bottom end of the floating platform and is tied on the bottom of the test box; the floating platform is hollow and cylindrical or in a truncated cone shape, and the floating state in the test box is adjusted by filling water into the inner cavity in advance; the test box is provided with a box cover, a plurality of tubular food grooves are formed in the box cover, food and drinking water bottles are used for being inserted into the food grooves to feed food and water for small animals, the bottom ends of the food grooves are designed to be inclined, and outlets for extending out of the food or flowing out of the water are formed in the side walls of the food grooves.
2. The experimental device according to claim 1, wherein the fixing frame is in a frame shape, a plurality of horizontal annular frames and supporting legs are arranged, and the bottom ends of the supporting legs are fixed on the bottom of the experimental box.
3. The experimental device according to claim 1, wherein the upper end surface of the floating platform is a rough surface or is provided with anti-skid grains; the floating platform is provided with 12, make unrestrained pump and be provided with 1, the floating platform is around make unrestrained pump setting.
4. The experimental device according to claim 1, wherein the test chamber is provided with a water inlet valve and a water outlet valve, and an outlet end of the water inlet valve is provided with a filter screen.
5. The assay device of claim 1 wherein said cover is covered with a shade cloth.
6. The experimental device according to claim 1, wherein the test chamber and the fixing frame are both made of stainless steel, and the fixing frame is fixed with the bottom of the test chamber by adopting a welding or screw connection structure.
7. The experimental set-up of claim 1, wherein the wave making pump power is adjustable; the wave making pump is provided with a plurality of wave making pumps.
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| CN201610196887.5A CN107280771B (en) | 2016-03-31 | 2016-03-31 | Life body experimental device for simulating ocean long-distance navigation state |
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| CN109528341B (en) * | 2018-11-15 | 2021-06-04 | 南方医科大学第三附属医院（广东省骨科研究院） | Molding equipment, molding method and application of mouse intervertebral disc degeneration model |
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