CN107271487B - Realize the device and method of live body nerve high-frequency electromagnetic conduction active probe - Google Patents
Realize the device and method of live body nerve high-frequency electromagnetic conduction active probe Download PDFInfo
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- CN107271487B CN107271487B CN201710400162.8A CN201710400162A CN107271487B CN 107271487 B CN107271487 B CN 107271487B CN 201710400162 A CN201710400162 A CN 201710400162A CN 107271487 B CN107271487 B CN 107271487B
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Abstract
The invention belongs to frequency electromagnetic waves in the conduction studies field that nervous system collects, the defects of for conventional direct probe method, there is provided a kind of device and method of live body nerve high-frequency electromagnetic conduction active probe, device includes high-frequency electromagnetic signal unit, optical fiber, detector, seal cavity, the curved road type neural channel in seal cavity, is arranged on and is used for the multiple supporting plates for supporting nerve samples in curved road type neural channel；It will be placed on the nerve samples handled well in curved road type neural channel, incident optical gently penetrated to one end of nerve so that incident frequency electromagnetic waves full illumination is in nerve, you can carry out the quick detection of high-frequency electromagnetic transport properties.The present invention can effectively solve the problems such as big incoming electromagnetic wave interference in the conduction detection experiment of neural high-frequency electromagnetic, neural easy in inactivation, realize the quick detection of live body nerve high-frequency electromagnetic conduction.
Description
Technical field
The invention belongs to detect neural high-frequency electromagnetic conduction studies field, more particularly to one kind realizes live body nerve high-frequency electrical
Magnetic conducts the device and method of active probe.
Background technology
Nervous system is the most complicated system of organism inner structure and function, risen in the vital movement of organism it is leading and
Adjustment effect, it is to receive outer signals, sensigenous, form consciousness, carry out logical thinking, sending instruction and produce in behavior
Pivot.
It is an important basic work to study propagation law of the frequency electromagnetic waves in nervous system.Detection nerve is high
The conventional method of frequency electromagnetic conductive is：By nerve samples directly through hollow sheet metal, high-frequency signal source and detector difference
Positioned at sample both ends, the high-frequency electromagnetic signal to be come using detector measurement through nerve conduction.This method is relatively simple, but deposits
In many problems, such as because detector and signal source are located on the same line, detector is easily done by incident frequency electromagnetic waves
Disturb, ambient noise it is strong；In addition, nerve samples are directly exposed in air, in the short time will dehydration it is dead, give experimental study band
Carry out very big uncertainty.
The content of the invention
In order to solve the problems such as big incoming electromagnetic wave interference in the conduction detection of neural high-frequency electromagnetic, neural easy in inactivation, this hair
It is bright to provide a kind of device and method of new live body nerve high-frequency electromagnetic conduction active probe, non-nerve conduction can be avoided to believe
Number interference brought into detector, effectively reduces noise；Simultaneously in experimentation, nerve samples can keep living for a long time
Property, the consumption of nerve samples is greatly reduced, effectively prevent the problem of nerve inactivation is brought.
The present invention technical solution be：
A kind of device for realizing live body nerve high-frequency electromagnetic conduction active probe, including high-frequency electromagnetic signal unit, optical fiber
1st, detector 2, the output end of above-mentioned high-frequency electromagnetic signal unit are connected with the incidence end of optical fiber 1,
It is characterized in that：
Also include seal cavity 3, the curved road type neural channel 31 in seal cavity 3, to be arranged on curved road type nerve logical
It is used for the multiple supporting plates 32 for supporting nerve samples in road 31；
Physiological fluid is full of in above-mentioned seal cavity 3 and is communicated with physiological fluid EGR；By physiological fluid recycle stream
Neural channel is crossed, keeps the activity of nerve samples；
Be provided with above-mentioned seal cavity 3 frequency electromagnetic waves input window 33 corresponding with the both ends of curved road type neural channel 31 and
Frequency electromagnetic waves output window 34；
Above-mentioned frequency electromagnetic waves input window 33 is provided with aperture, and the exit end of above-mentioned optical fiber 1 inputs through frequency electromagnetic waves
Enter curved road type neural channel 31 after the aperture of window 33；
The above-mentioned face detector input window of frequency electromagnetic waves output window 34.
Preferably, in order to reduce ambient noise, detection device of the present invention also includes perforated metal baffle plate 4；Above-mentioned gold with holes
Belong to baffle plate 4 between frequency electromagnetic waves output window 34 and detector input window.
Preferably, in order to further reduce ambient noise, above-mentioned supporting plate 32 includes substrate 321, positioned at substrate 321
The substrate 322 of upper surface, the nano column array for being arranged on the surface of substrate 322.
Preferably, the nano-pillar 323 in above-mentioned nano column array is cone, and the above-mentioned pillar height of nano-pillar 323 is micron order, cone
The top end diameter of body is 7-13nm.
Preferably, above-mentioned nano-pillar 323 uses multiphase carbon film material, and substrate 322 uses crosslinked polystyrene or poly- carbonic acid
Ester；Or nano-pillar 323 uses multiphase silicon fiml material, substrate 322 uses silica and silicon.
Preferably, the thickness of above-mentioned frequency electromagnetic waves input window 33 and frequency electromagnetic waves output window 34 is 0.9-1.1mm；When
When frequency electromagnetic waves are 0.8um-300um all bands, frequency electromagnetic waves input window 33 and frequency electromagnetic waves output window 34 are using gold
Hard rock material；When frequency electromagnetic waves are 0.8um-14um wave bands, frequency electromagnetic waves input window 33 and frequency electromagnetic waves output window
34 use barium fluoride material；When frequency electromagnetic waves are 14um-300um wave bands, frequency electromagnetic waves input window 33 and high-frequency electromagnetic
Ripple output window 34 uses high density polyethylene (HDPE) or TPX materials (4- methylpentene polymers).
Preferably, in order to enter nerve samples for direct high-frequency electromagnetic wave, the coupling efficiency of frequency electromagnetic waves is improved, on
It is a diameter of millimetre-sized broadband optical fiber to state optical fiber 1, using unorganic glass optical fiber or micro-structural organic polymer optical fiber.
Preferably, in order to reduce the ambient noise that incident electromagnetic wave is brought, the turning angle of above-mentioned curved road type neural channel 31
Spend for 90 degree；Above-mentioned detector 2 is low-temp radiating heat meter；90 degree of bends make it that incident frequency electromagnetic waves and detection direction are orthogonal,
So as to reduce the ambient noise that incident electromagnetic wave is brought.
The present invention also provides the above-mentioned method for realizing live body nerve high-frequency electromagnetic conduction active probe, comprises the following steps：
Step 1：Pre-process nerve samples；
Step 2：The nerve samples handled well are placed in curved road type neural channel 31 and in physiological cycle liquid；
Step 3：The exit end of optical fiber 1 penetrates one end of nerve samples；
Step 4：Frequency electromagnetic waves are coupled into nerve samples through optical fiber 1；
Step 5：Detector 2 receives high-frequency electromagnetic signal from the nerve samples other end.
Preferably, in above-mentioned steps 1, the specific method for pre-processing nerve samples is successively in low temperature and normal temperature mechanical brains ridge
Nerve samples are carried out continuing oxygen supply until its recovery in liquid；
Preferably, in above-mentioned steps 2, above-mentioned nerve samples are propped up by multiple supporting plates 32 in curved road type neural channel 31
Support.
The beneficial effects of the invention are as follows：
1st, live body nerve high-frequency electromagnetic conduction active probe device provided by the present invention is logical by 90 degree of curved road type nerves
Road make it that incident frequency electromagnetic waves are orthogonal with detector direction, it is possible to reduce ambient noise, efficiently solves neural high-frequency electromagnetic
The problems such as incoming electromagnetic wave interference is big in conduction detection.
2nd, the present invention uses multiple supporting plates in nerve samples bottom so that nerve is immersed in oxygen-enriched physiological fluid, branch
Fagging surface carries the nanoforest structure that nano column array is formed, and for absorbing ambient noise, can equally reduce background and make an uproar
Sound.
3rd, the present invention is by setting the oxygen-enriched physiological fluid of the physiological fluid circulatory system to cycle through nerve samples, effectively
Solves the problems such as neural easy in inactivation in neural high-frequency electromagnetic conduction detection.
Brief description of the drawings
Fig. 1 is that live body nerve high-frequency electromagnetic conducts active probe device；
Fig. 2 a are that live body nerve high-frequency electromagnetic conduction active probe device removes structural representation after top cover；
Fig. 2 b are that live body nerve high-frequency electromagnetic conduction active probe device removes structural representation after bottom plate；
Fig. 2 c are that Fig. 1 live body nerves high-frequency electromagnetic conducts active probe device left view；
Fig. 3 is supporting plate structure schematic diagram；
Reference is：1- optical fiber, 2- detectors, 3- seal cavities, 31- curved road type neural channels, 32- supporting plates,
321- substrates, 322- substrates, 323- nano-pillars, 33- frequency electromagnetic waves input windows, 34- frequency electromagnetic waves output windows, 35- physiology
Liquid circulating apparatus, 4- perforated metal baffle plates.
Embodiment
With reference to the accompanying drawings and examples, the embodiment of the present invention is described further.
The live body nerve high-frequency electromagnetic conduction active probe device that the present embodiment provides as can be seen from Figure 1, including high frequency
Electromagnetic signal unit (not shown), optical fiber 1, seal cavity 3, perforated metal baffle plate 4, detector 2, high-frequency electromagnetic signal list
The output end of member is connected with optical fiber 1, and curved road type neural channel 31 is provided with seal cavity 3, is set in curved road type neural channel 31
Multiple supporting plates 32 for supporting nerve samples are equipped with, physiological fluid and itself are full of in seal cavity 3 and physiological fluid circulates
Device 35 is communicated, and the circulation of physiological fluid is realized using peristaltic pump.
Curved road type neural channel 31 can be set in the following way：Dividing plate is set in seal cavity 3, by seal cavity 3
It is divided into two layers up and down, lower floor is communicated full of physiological fluid and with physiological fluid EGR 35, and sinking is offered on dividing plate
Formula curved road type neural channel 31, curved road type neural channel 31 is communicated with lower floor, and its bottom, which is provided with, to be used to support the more of nerve samples
Individual supporting plate 32, lower floor's physiological fluid can not have supporting plate 32 to make nerve samples positioned at being immersed in oxygen-enriched physiological fluid.
Supporting plate 32 includes substrate 321, the substrate 322 positioned at the upper surface of substrate 321, and substrate surface is provided with nano-pillar
The 323 nanoforest structures formed, as shown in figure 3, for absorbing ambient noise；Nano-pillar 323 is cone, and pillar height is micron
Grade, it is 2 μm in the present embodiment, a diameter of 10nm of spherical or plane capital or so；When the selection of nano-pillar 323 multiphase carbon film
During material, the selection crosslinked polystyrene of substrate 322 or makrolon.When the selection of nano-pillar 323 is with multiphase silicon fiml material, lining
The selection silica of bottom 322 and silicon.
1 a diameter of grade of broadband input optical fibre, when frequency electromagnetic waves are in 0.8um-14um Frequency Band Selection unorganic glasses
Optical fiber, when frequency electromagnetic waves 14um-300um frequency ranges may be selected use micro-structural organic polymer optical fiber.Highly-sensitive detector,
Selection low-temp radiating heat meter, peak detectivity D* is up to 1013cmHz1/2W-1。
The frequency electromagnetic waves input window 33 and high frequency with the both ends face of curved road type neural channel 31 are provided with seal cavity 3
Electromagnetic wave output window 34；Frequency electromagnetic waves input window 33 and frequency electromagnetic waves output window 34 use frequency electromagnetic waves high transmittance
Material, for sealing physiological fluid up for safekeeping, while ensure that frequency electromagnetic waves have high penetrance；Frequency electromagnetic waves input window 33 is provided with
Aperture, size are slightly larger than the diameter of optical fiber 1 so that incident optical 1 is passed through and is in turn coupled on nerve samples；Frequency electromagnetic waves are defeated
The rear end of exit window 34 is perforated metal baffle plate 4, frequency electromagnetic waves detector 21 is terminated after perforated metal baffle plate 4, for detecting through god
The high-frequency electromagnetic signal conducted through sample, perforated metal baffle plate 4 are used to stop that other interference enter detector；So as to ensure to detect
The low noise and high sensitivity of device.
The fresh and alive bullfrog sciatic nerve that will have been dissected in experiment, is successively persistently supplied oxygen in low temperature and normal temperature artificial cerebrospinal fluid
Recovery, is then placed within 90 degree of curved road type neural channels 31, and incident optical is gently penetrated to one end of nerve so that incident high
Frequency electromagnetic waves full illumination is in nerve, you can carries out the quick detection of high-frequency electromagnetic transport properties.
Claims (10)
1. a kind of device for realizing live body nerve high-frequency electromagnetic conduction active probe, including high-frequency electromagnetic signal unit, optical fiber
(1), detector (2), the output end of the high-frequency electromagnetic signal unit are connected with optical fiber (1) incidence end,
It is characterized in that：
Also include seal cavity (3), the curved road type neural channel (31) in seal cavity (3), be arranged on curved road type nerve
It is used for the multiple supporting plates (32) for supporting nerve samples in passage (31)；
Physiological fluid is full of in the seal cavity (3) and is communicated with physiological fluid EGR (35)；
Seal cavity (3) surface is provided with the frequency electromagnetic waves input window with curved road type neural channel (31) both ends face
And frequency electromagnetic waves output window (34) (33)；
The frequency electromagnetic waves input window (33) is provided with aperture, and the exit end of the optical fiber (1) inputs through frequency electromagnetic waves
Enter curved road type neural channel (31) after the aperture of window (33)；
Frequency electromagnetic waves output window (34) the face detector input window.
2. the device according to claim 1 for realizing live body nerve high-frequency electromagnetic conduction active probe, it is characterised in that：Also
Including perforated metal baffle plate (4)；The perforated metal baffle plate (4) is located at frequency electromagnetic waves output window (34) and detector input window
Between.
3. the device according to claim 1 or 2 for realizing live body nerve high-frequency electromagnetic conduction active probe, its feature exist
In：The supporting plate (32) includes substrate (321), the substrate (322) positioned at substrate (321) upper surface, is arranged on substrate (322)
The nano column array of upper surface.
4. the device according to claim 3 for realizing live body nerve high-frequency electromagnetic conduction active probe, it is characterised in that：Institute
It is cone to state the nano-pillar (323) in nano column array, and nano-pillar (323) pillar height is micron order, the top end diameter of cone
For 7-13nm.
5. the device according to claim 3 for realizing live body nerve high-frequency electromagnetic conduction active probe, it is characterised in that：Institute
State nano-pillar (323) and use multiphase carbon film material, substrate (322) uses crosslinked polystyrene or makrolon；Or nano-pillar
(323) multiphase silicon fiml material is used, substrate (322) uses silica and silicon.
6. the device according to claim 1 or 2 for realizing live body nerve high-frequency electromagnetic conduction active probe, its feature exist
In：
The thickness of the frequency electromagnetic waves input window (33) and frequency electromagnetic waves output window (34) is 0.9-1.1mm；
When frequency electromagnetic waves are 0.8um-300um all bands, frequency electromagnetic waves input window (33) and frequency electromagnetic waves output window
(34) diamond is used；
When frequency electromagnetic waves are 0.8um-14um wave bands, frequency electromagnetic waves input window (33) and frequency electromagnetic waves output window (34)
Using barium fluoride material；
When frequency electromagnetic waves are 14um-300um wave bands, frequency electromagnetic waves input window (33) and frequency electromagnetic waves output window (34)
Using high density polyethylene (HDPE) or TPX materials.
7. the device according to claim 1 or 2 for realizing live body nerve high-frequency electromagnetic conduction active probe, its feature exist
In：The optical fiber (1) is a diameter of millimetre-sized broadband optical fiber, using unorganic glass optical fiber or micro-structural organic polymer object light
It is fine.
8. the device according to claim 1 or 2 for realizing live body nerve high-frequency electromagnetic conduction active probe, its feature exist
In：
The angle of turn of the curved road type neural channel (31) is 90 degree；The detector (2) is low-temp radiating heat meter.
9. realizing the method for live body nerve high-frequency electromagnetic conduction active probe based on any described device of claim 1 to 8, it is special
Sign is：Comprise the following steps,
Step 1：Pre-process nerve samples；
Step 2：The nerve samples handled well are placed in curved road type neural channel (31) and in physiological cycle liquid；
Step 3：The exit end of optical fiber (1) penetrates one end of nerve samples；
Step 4：Frequency electromagnetic waves are coupled into nerve samples through optical fiber (1)；
Step 5：Detector (2) receives high-frequency electromagnetic signal from the nerve samples other end.
10. the method for live body nerve high-frequency electromagnetic conduction active probe is realized according to claim 9, it is characterised in that：
In the step 1, the specific method for pre-processing nerve samples is successively in low temperature and normal temperature artificial cerebrospinal fluid to nerve
Sample carries out continuing oxygen supply until its recovery；
In the step 2, the nerve samples are supported by multiple supporting plates (32) in curved road type neural channel (31).
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| CN109557092B (en) * | 2018-11-22 | 2019-12-31 | 中国人民解放军军事科学院国防科技创新研究院 | Cranial nerve stimulation device for enhancing cognitive function of brain |
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|---|---|---|---|---|
| CN2824837Y (en) * | 2005-08-12 | 2006-10-11 | 成都仪器厂 | Experimental apparatus for electrophysiological examination |
| CN102279208A (en) * | 2011-06-24 | 2011-12-14 | 中国人民解放军军事医学科学院基础医学研究所 | Analysis system for electromagnetic radiation effect based on microelectrode array neural chip |
| CN103454314A (en) * | 2013-09-02 | 2013-12-18 | 中国人民解放军第三军医大学 | Electrophysiological comprehensive experiment shielding box |
| CN106596643A (en) * | 2016-12-06 | 2017-04-26 | 成都泰盟软件有限公司 | Device for maintaining neural activity in nerve electronic shielding testing process |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| TWI498101B (en) * | 2012-08-30 | 2015-09-01 | Univ Nat Chiao Tung | Method of analyzing nerve fiber distribution and measuring standardized induced compound motion electric potential |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2824837Y (en) * | 2005-08-12 | 2006-10-11 | 成都仪器厂 | Experimental apparatus for electrophysiological examination |
| CN102279208A (en) * | 2011-06-24 | 2011-12-14 | 中国人民解放军军事医学科学院基础医学研究所 | Analysis system for electromagnetic radiation effect based on microelectrode array neural chip |
| CN103454314A (en) * | 2013-09-02 | 2013-12-18 | 中国人民解放军第三军医大学 | Electrophysiological comprehensive experiment shielding box |
| CN106596643A (en) * | 2016-12-06 | 2017-04-26 | 成都泰盟软件有限公司 | Device for maintaining neural activity in nerve electronic shielding testing process |
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