CN205286322U - Biological tissue optoacoustic circulation tumor cells detection diagnosis device - Google Patents
Biological tissue optoacoustic circulation tumor cells detection diagnosis device Download PDFInfo
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- CN205286322U CN205286322U CN201521015853.9U CN201521015853U CN205286322U CN 205286322 U CN205286322 U CN 205286322U CN 201521015853 U CN201521015853 U CN 201521015853U CN 205286322 U CN205286322 U CN 205286322U
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Abstract
The utility model discloses a biological tissue optoacoustic circulation tumor cells detection diagnosis device, the device are equipped with high frequency pulse laser, microscope and ultrasonic probe, will be detected the thing and arrange in between microscope and the ultrasonic probe, make laser focusing that the microscope camera lens produced high frequency pulse laser on being detected the thing. Adopt above -mentioned technical scheme, overcome the fluorescent label thing and produce the shortcoming of disturbing, producing cytotoxicity to normal blood cell, lymphocyte to the biological tissue physiological environment, accomplish non -invasive detection, need not draw blood, without the mark, avoided the data difference between the animal individuality, required animal number among the animal experiment has significantly reduced, the usage is extensive, but wide application in clinical medicine and foundation medicine research fields such as immunology, hematology, oncology, cell biology, cytogenetics, biochemistry, simple, the convenient operation of equipment structure, security height.
Description
Technical field
The utility model belongs to medical research and the technical field of diagnostic instrments equipment. More specifically, the utility model relates to biological tissue's light sound circulating tumor cell checkout and diagnosis device.
Background technology
Cancer is the disease of serious threat human health and life, cancer patient if in early days find and treat in time, be possible not only to improve survival rate, the life quality of patient can also be improved simultaneously, thus for cancer want early discovery, early diagnosis and treat in time.
In recent years, the development of the optics early diagnosis technology of cancer, comprises photoacoustic imaging(PAI), light sound fault imaging, optoacoustic spectroscopy microscope imaging etc. Such as, flow cytomery technology mainly comprises external flow cytometer, living body fluorescent mark flow cytometer, live body light sound/photo-thermal flow cytometer etc. Living body fluorescent mark flow cytometer overcomes the shortcoming of the accurate quantitative analysis to cell number required for confocal/two-photon microscope can not provide many studies and clinical applications further, it is widely used in the Real-Time Monitoring of the high resolving power/low background of single living cell, and the biomolecules in research live body changes and bioprocess, but the circulating cells being through fluorescent mark of detection. Fluorescent marker has cytotoxicity, it is injected into again in body after needing intravenous injection or sampling external dyeing, cause tissue injury, likely affect cell physiological environment in vivo, also pollution is easily introduced after dyeing during injection, therefore, monitoring frequency, the monitoring periods of same biont are had certain restriction by this technology.
How to overcome fluorescent mark to shortcomings such as the interference of physiological environment and cytotoxicities, safer, non-invasively meet the Real-Time Monitoring to live body inner cell number and quantitative analysis is a problem demanding prompt solution.
Practical novel content
The utility model provides a kind of biological tissue light sound circulating tumor cell checkout and diagnosis device, its objective is to realize Non-invasive detection, makes the early diagnosis of the multiple diseases such as cancer safer.
In order to realize above-mentioned purpose, the technical scheme that the utility model is taked is:
Biological tissue of the present utility model light sound circulating tumor cell checkout and diagnosis device, it is provided with high-frequency impulse laser apparatus, microscope and ultrasound probe, detected material is placed between microscope and ultrasound probe, makes laser focusing that high-frequency impulse laser apparatus is produced by described microscope camera lens on detected material.
Described detected material is animal blood vessels.
Between described high-frequency impulse laser apparatus and microscope, convex lens, concavees lens and slit are set; The laser that described high-frequency impulse laser apparatus produces, successively through convex lens, concavees lens and slit shaping, enters described microscope.
Described ultrasound probe is connected with signal handling equipment by ultrasonic signal circuit.
Described signal handling equipment is connected with indicating system by display circuit.
The optical maser wavelength of described high-frequency impulse laser apparatus is 1064nm; Or it is 808nm.
The type of described high-frequency impulse laser apparatus is the one in solid statelaser, semiconductor laser, gas laser, optical fiber laser and dye laser; The pattern of its Laser output is pulse laser.
Described ultrasound probe is the one in monocrystalline compressional wave straight beam probe, twin crystal compressional wave straight beam probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface-duided wave probe.
The range of frequency of described ultrasound probe is 1-100MHz.
In order to realize the goal of the invention identical with technique scheme, the utility model additionally provides the checkout and diagnosis method being applied to above-described biological tissue light sound circulating tumor cell checkout and diagnosis device, and its technical scheme is:
The laser that described high-frequency impulse laser apparatus sends is by the blood vessel on microscope lens focus such as mouselet ear inner skin top layer on the suitable blood vessel of living small animal;
When the target cell in the recycle system is by focusing point (form) of laser at this blood vessel, being subject to the irradiation of the high-frequency impulse laser of different wave length, produce photoacoustic waves, the ultrasound probe that its ultrasonic signal sent is placed on tissue surface detects;
Then the ultrasonic signal of the circulation target cell detected is processed and at the cell number of focusing point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit time by signal handling equipment, and process data show on the display system in real time.
The useful effect of the utility model employing technique scheme is:
1, on the basis of living body fluorescent detection method, in conjunction with the photoacoustic effect of biological tissue, utilize the light sound specificity of biological tissue and the deep penetration of ultrasonic signal, overcome fluorescent marker to biological tissue's physiological environment generation interference, to the shortcoming of normal plasma cell, lymphocyte generation cytotoxicity;
2, Non-invasive detection can be accomplished, need not draw blood, need not mark;
3, in the advantage of (work) body Real-Time Monitoring, for a long time same animal individual can be carried out performance analysis, avoid the data difference between animal individual, thus greatly reduce animal number required in experimentation on animals;
4, no cytotoxicity big without the need to mark, penetration depth;
5, this device purposes is extensive: can be widely used in clinical medicine and the basic medical research fields such as immunology, hematology, oncology, cytobiology, cytogenetics, biological chemistry;
6, this device structure is simple, handled easily, Wicresoft, security height;
7, adopt liquid-crystal display, have display quality height, digital interface, volume is little, weight is light and the advantage such as low in energy consumption.
Accompanying drawing explanation
Accompanying drawing content and figure mark brief description as follows:
Fig. 1 is the schematic diagram of smooth sound circulating tumor cell checkout and diagnosis device of the present utility model.
Figure is labeled as:
1, high-frequency impulse laser apparatus, 2, microscope, 3, ultrasound probe, 4, signal handling equipment, 5, indicating system, 6, convex lens, 7, concavees lens, 8, slit, 9, animal blood vessels.
Embodiment
Compareing accompanying drawing below, by the description to embodiment, embodiment of the present utility model be described in further detail, to help, invention of the present utility model is conceived by the technician of this area, technical scheme has more complete, accurate and deep understanding.
The utility model structure as shown in Figure 1 is a kind of biological tissue light sound circulating tumor cell checkout and diagnosis device; The utility model also relates to this device tumour cell checkout and diagnosis method, especially in vivo cell unmarked catch, the checkout and diagnosis method of Real-Time Monitoring and quantitative analysis and a device, utilize the metastatic cancer cell in the light sound specificity of biological tissue and the deep penetration detection circulation of blood of ultrasonic signal.
In order to solve prior art Problems existing and overcome its defect, it is achieved Non-invasive detection, the goal of the invention making the early diagnosis of the multiple diseases such as cancer safer, the technical scheme that the utility model is taked is:
As shown in Figure 1, biological tissue of the present utility model light sound circulating tumor cell checkout and diagnosis device, it is provided with high-frequency impulse laser apparatus 1, microscope 2 and ultrasound probe 3, detected material is placed between microscope 2 and ultrasound probe 3, makes laser focusing that high-frequency impulse laser apparatus 1 is produced by described microscope 2 camera lens on detected material.
The utility model is for prior art Problems existing, it is proposed in the technical scheme of (work) body light sound circulating tumor cell checkout and diagnosis device. (work) body light sound tumour cell detect device be the high-frequency impulse laser that a branch of high-frequency impulse laser apparatus 1 is produced by microscope 2 lens focus on the suitable blood vessel (such as the blood vessel on mouselet ear inner skin top layer) of living small animal;
When the target cell (in such as circulating tumor cell, hemocyte, lymphocyte and blood other cell) in the recycle system is by focusing point (form) of laser at this blood vessel, it is subject to the irradiation of the high-frequency impulse laser of different wave length, producing photoacoustic waves, the ultrasound probe 3 that its ultrasonic signal sent can be placed on tissue surface detects;
Then the ultrasonic signal of the circulation target cell detected is processed and at the cell number of focusing point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit time (such as every minute) by signal handling equipment 4, and process data are displayed in indicating system 5 in real time.
Can be applicable to the cell contrast medium in detection circulation and pathogenic agent etc. at (work) body light sound tumour cell detector. For numerous disease as the early diagnosis of cancer, diabetes and heart trouble etc. brings hope, also for multiple factor such as medicine, smoking, radiation etc., the influence research of cell is provided new method.
Described detected material is animal blood vessels 9.
Spot shaping system:
Between described high-frequency impulse laser apparatus 1 and microscope 2, convex lens 6, concavees lens 7 and slit 8 are set; The laser that described high-frequency impulse laser apparatus 1 produces, successively through convex lens 6, concavees lens 7 and slit 8 shaping, enters described microscope 2.
Laser facula, by carrying out shaping after convex lens 6, concavees lens 7, slit 8, is shaped to strip and other shape by the exciting light of described high-frequency impulse laser apparatus 1, in order to cover animal blood vessels 9.
This spot shaping system, it is possible to be set of lenses, it is also possible to be single lens. The lens focus of microscope 2 is (such as the blood vessel on mouselet ear inner skin top layer) on the suitable blood vessel of living small animal;
When the target cell (in such as circulating tumor cell, hemocyte, lymphocyte and blood other cell) in the recycle system is by focusing point (form) of laser at this blood vessel, the irradiation being subject to the high-frequency impulse laser of different wave length, produces photoacoustic waves. This microscope is the optical lens that light is had focusing function by microcobjective, inversion microcobjective etc.
Described ultrasound probe 3 is connected with signal handling equipment 4 by ultrasonic signal circuit.
Described signal handling equipment 4 is connected with indicating system 5 by display circuit.
The ultrasonic signal that described ultrasound probe 3 detects, is processed by signal handling equipment 4.
The ultrasonic signal of the circulation target cell detected is processed and at the cell number of focusing point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit time (such as every minute) by signal handling equipment 4.
Its result shows on the display system, and this indicating system can be all kinds of indicating system such as LD indicating meter, LED indicating meter.
The optical maser wavelength of described high-frequency impulse laser apparatus 1 can be: the wavelength that 1064nm, 808nm etc. are different.
The type of described high-frequency impulse laser apparatus 1 is the one in solid statelaser, semiconductor laser, gas laser, optical fiber laser and dye laser; The pattern of its Laser output is pulse laser.
Described ultrasound probe 3 is the one during the model such as monocrystalline compressional wave straight beam probe, twin crystal compressional wave straight beam probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface-duided wave probe is popped one's head in.
The range of frequency of ultrasound probe 3 is 1-100MHz, or other frequency range.
In order to realize the goal of the invention identical with technique scheme, the utility model additionally provides the checkout and diagnosis method of above-described biological tissue light sound circulating tumor cell checkout and diagnosis device, and its technical scheme is:
The laser that described high-frequency impulse laser apparatus 1 sends by microscope 2 lens focus on the suitable blood vessel of living small animal (such as the blood vessel on mouselet ear inner skin top layer);
When the target cell (in such as circulating tumor cell, hemocyte, lymphocyte and blood other cell) in the recycle system is by focusing point (form) of laser at this blood vessel, it is subject to the irradiation of the high-frequency impulse laser of different wave length, producing photoacoustic waves, the ultrasound probe 3 that its ultrasonic signal sent is placed on tissue surface detects;
Then the ultrasonic signal of the circulation target cell detected is processed and at the cell number of focusing point (form) of this blood vessel, is carried out quantitative analysis (counting) by laser the unit time (such as every minute) by signal handling equipment 4, and process data are displayed in indicating system 5 in real time.
Application example:
What the high-frequency impulse laser apparatus 1 of checkout and diagnosis device adopted is 1064nm pulsed laser, its repetition rate > 50kHz, maximum impulse energy: 50uJ;
Microscope 2 is inverted telescope, it is achieved the collimation of Gaussian beam;
Ultrasound probe 3 is 10MHz, the non-focus supersonic the being probe of employing;
The maximum sampling rate of signal handling equipment 4 can reach the data collecting card of 200MS/s, the common computer LED display that indicating system 5 adopts.
Utilizing said system to be detected by the skin carcinoma circulating tumor cell in mouse body, experiment obtains good effect, reaches re-set target.
Above in conjunction with accompanying drawing, the utility model is exemplarily described; obvious the utility model specific implementation is not subject to the restrictions described above; as long as have employed the improvement of the various unsubstantialities that method of the present utility model design carries out with technical scheme; or without improving, design of the present utility model and technical scheme directly applied to other occasion, all within protection domain of the present utility model.
Claims (8)
1. biological tissue's light sound circulating tumor cell checkout and diagnosis device, it is characterized in that: described checkout and diagnosis device is provided with high-frequency impulse laser apparatus (1), microscope (2) and ultrasound probe (3), detected material is placed between microscope (2) and ultrasound probe (3), makes laser focusing that high-frequency impulse laser apparatus (1) is produced by described microscope (2) camera lens on detected material.
2. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: described detected material is animal blood vessels (9).
3. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterized in that: between described high-frequency impulse laser apparatus (1) and microscope (2), convex lens (6), concavees lens (7) and slit (8) are set; The laser that described high-frequency impulse laser apparatus (1) produces, successively through convex lens (6), concavees lens (7) and slit (8) shaping, enters described microscope (2).
4. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: described ultrasound probe (3) is connected with signal handling equipment (4) by ultrasonic signal circuit.
5. according to biological tissue according to claim 4 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: described signal handling equipment (4) is connected with indicating system (5) by display circuit.
6. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: the optical maser wavelength of described high-frequency impulse laser apparatus (1) is 1064nm; Or it is 808nm.
7. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: the type of described high-frequency impulse laser apparatus (1) is the one in solid statelaser, semiconductor laser, gas laser, optical fiber laser and dye laser; The pattern of its Laser output is pulse laser.
8. according to biological tissue according to claim 1 light sound circulating tumor cell checkout and diagnosis device, it is characterised in that: described ultrasound probe (3) is monocrystalline compressional wave straight beam probe, twin crystal compressional wave straight beam probe, monocrystalline transverse wave double-bevel detector, twin crystal transverse wave double-bevel detector, circumferential curvature probe, radial buckling probe, point focusing probe, line focus surface-duided wave pop one's head in one.
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| CN201521015853.9U Active CN205286322U (en) | 2015-09-28 | 2015-12-07 | Biological tissue optoacoustic circulation tumor cells detection diagnosis device |
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| WO2023028835A1 (en) * | 2021-08-31 | 2023-03-09 | 深圳迈瑞动物医疗科技股份有限公司 | Specimen analysis apparatus and specimen analysis method |
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